CSPI03G02020 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G02020
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp
Location	Chr3: 1486097 .. 1487875 (+)
RNA-Seq Expression	CSPI03G02020
Synteny	CSPI03G02020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGGAAAGATCGGAGCAAAGGGCTCTAATTTAAGTGTAAGTAGTTGTAAGTAAACATATGTTGATATTAATTTGCAGATTGGTTTCAATGGTGGGATTCCAATTGACGAAGAGTTTTACATTAAGAATATTGCTGGCAAGAACAATGACGGTATTATCCGAACCCTATTCCCGAATGATGTCGAACAAGGTGCAGAATATATGAATGAGAAGGAGGCTATGTTTAGGAGGTAATAAAATATTTATCTTTATATACTTTCGATAATATATCATTATAAGTTTTGATTGAGACAACATATAACTAATGAATTTAACGTAGCCATCTAATTTTTTCCAAACTTACCTGAGATCATTACTGATCTATCTTAACTTTCCAAAAAAATCATAAAATAAAATAGTTCTCATATGGTCAGTCACAGATATCATTGATCTTCTTATTGTTCACCAGCTTGACTTTCCACTTACTTTTTTTTTTTCTTCACGATTCATGCATTCGAATGTCAATTATAAACTCAAATAATAATAATAATAAGGAAAATTTTCAAATGTAGTAAATTTTACAAAATATTTATACTGTGAATATAACAAAATCACTAAAAAGATCAAATTTATTACACTTGATTATGTTTGTATGAAGGGTAAATATTTTGTGAAATGTTTTATTTTTTATAATTTTTATAATATTTATCATTATTATTTCTTTTTCTTTCCCGATATATATAATACAAATACACATATATTTTTATTAGCATTCTCTTTGCTCAATTCCTTTATCTCTTCTCCAATCAACGTTATTCATCTCTCACTCGTTAACTCATCTTATTTTTCAATAGTTACAACTATATTGTAGGGACATTTTTAAAAATAGAATAATAAATTAAAATATTTACAAGCTATAACAAAATTTTGGATTCTATCACAAATAGTGTTTTATCACTATAGCATATCAATAACTATTACAGATAGAATTTGCTATAGGTGTTGTAAATATCTTGTAAGATCTACTATTTTTAAAAATTCTCCTATATTATATTCTCTATAATTAATTTTCTTTTCCATATTAACTATTTATACAAAACCAAATAATTAATATCATATTCCACCTAAACAAACTTTTCCTCTCAAAATCCCAAATCAACTCAAACTCTCTAACTCTTTTAATTAACCAAATCTTCTACTTTTAATATTTGATAAATAATTTAAGATACAAATTTGGTAAAAATTTGAGATGTTTCACTTAATGTAACAGATTAGTTGTTGAACAATTAAAGCCGGTGAGTGGCTTGTATAAAGTGAAGAAATGGGTGGAAGATCACAGATTGAAATGTGCTGCAGTTACGAACTCCTCTCGAATTAATGCCGAGCTCATGATCTCGATGCTCGGTCTTACGGATTTTTTCGAGACTATTATTATAGGTGATGAATGTGAGCACCCCAAACCACATCCAGAACCATATTTAAAGGCTTTGGAAGCACTCCAAGTATCAAAGGATCACACTTTCATTTTTGAGGTCAGTCATATGAAATTTTCTTTTCTCCCCATTGTTTTGTTCGAAATTTGAAAACTTGAATGCTTTGCTTTGCTAGGATTCTCTAACAGGGATCAAAGCTGGAGTTGAAGCCGATATGCCTGTTGTCGGTATATCAATGAGAAATTCTGAGCAGTTGTTGATGCAAGCAAAGCCAGCCCTCCTCGTAAAAGACTACAACGATTTGAAATTGTGGTTAGCTTTGGACGAACTAGTTGTGAGAGGAGATACCATTGCAAATGCATGA

mRNA sequence

ATGAAAGGAAAGATCGGAGCAAAGGGCTCTAATTTAAGTATTGGTTTCAATGGTGGGATTCCAATTGACGAAGAGTTTTACATTAAGAATATTGCTGGCAAGAACAATGACGGTATTATCCGAACCCTATTCCCGAATGATGTCGAACAAGGTGCAGAATATATGAATGAGAAGGAGGCTATGTTTAGGAGATTAGTTGTTGAACAATTAAAGCCGGTGAGTGGCTTGTATAAAGTGAAGAAATGGGTGGAAGATCACAGATTGAAATGTGCTGCAGTTACGAACTCCTCTCGAATTAATGCCGAGCTCATGATCTCGATGCTCGGTCTTACGGATTTTTTCGAGACTATTATTATAGGTGATGAATGTGAGCACCCCAAACCACATCCAGAACCATATTTAAAGGCTTTGGAAGCACTCCAAGTATCAAAGGATCACACTTTCATTTTTGAGGATTCTCTAACAGGGATCAAAGCTGGAGTTGAAGCCGATATGCCTGTTGTCGGTATATCAATGAGAAATTCTGAGCAGTTGTTGATGCAAGCAAAGCCAGCCCTCCTCGTAAAAGACTACAACGATTTGAAATTGTGGTTAGCTTTGGACGAACTAGTTGTGAGAGGAGATACCATTGCAAATGCATGA

Coding sequence (CDS)

ATGAAAGGAAAGATCGGAGCAAAGGGCTCTAATTTAAGTATTGGTTTCAATGGTGGGATTCCAATTGACGAAGAGTTTTACATTAAGAATATTGCTGGCAAGAACAATGACGGTATTATCCGAACCCTATTCCCGAATGATGTCGAACAAGGTGCAGAATATATGAATGAGAAGGAGGCTATGTTTAGGAGATTAGTTGTTGAACAATTAAAGCCGGTGAGTGGCTTGTATAAAGTGAAGAAATGGGTGGAAGATCACAGATTGAAATGTGCTGCAGTTACGAACTCCTCTCGAATTAATGCCGAGCTCATGATCTCGATGCTCGGTCTTACGGATTTTTTCGAGACTATTATTATAGGTGATGAATGTGAGCACCCCAAACCACATCCAGAACCATATTTAAAGGCTTTGGAAGCACTCCAAGTATCAAAGGATCACACTTTCATTTTTGAGGATTCTCTAACAGGGATCAAAGCTGGAGTTGAAGCCGATATGCCTGTTGTCGGTATATCAATGAGAAATTCTGAGCAGTTGTTGATGCAAGCAAAGCCAGCCCTCCTCGTAAAAGACTACAACGATTTGAAATTGTGGTTAGCTTTGGACGAACTAGTTGTGAGAGGAGATACCATTGCAAATGCATGA

Protein sequence

MKGKIGAKGSNLSIGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPVSGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPYLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYNDLKLWLALDELVVRGDTIANA*

Homology

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BLAST of CSPI03G02020 vs. ExPASy Swiss-Prot
Match: Q9ZVJ5 (Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Arabidopsis thaliana OX=3702 GN=SGPP PE=1 SV=2)

HSP 1 Score: 253.4 bits (646), Expect = 2.2e-66
Identity = 116/190 (61.05%), Postives = 152/190 (80.00%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFN G+PIDE+F+++NIAGK+N  I   LFP+DV +G ++ +EKEA++R++V E++KP+
Sbjct: 51  IGFNNGVPIDEKFFVENIAGKHNSEIALLLFPDDVSRGLKFCDEKEALYRKIVAEKIKPL 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
            GL K+ KW+ED  LK AAVTN+ + NAELMIS LGLTDFF+ +I+G ECE PKPHP PY
Sbjct: 111 DGLIKLTKWIEDRGLKRAAVTNAPKENAELMISKLGLTDFFQAVILGSECEFPKPHPGPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L VSK+HT +FEDS++GIKAGV A MPV+G++  N   LLMQAKPA L+++Y D
Sbjct: 171 LKALEVLNVSKEHTLVFEDSISGIKAGVAAGMPVIGLTTGNPASLLMQAKPAFLIENYAD 230

Query: 194 LKLWLALDEL 204
            KLW  L+EL
Sbjct: 231 PKLWAVLEEL 240

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BLAST of CSPI03G02020 vs. ExPASy Swiss-Prot
Match: Q9K6Y7 (Pyrophosphatase PpaX OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=ppaX PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-11
Identity = 52/159 (32.70%), Postives = 79/159 (49.69%), Query Frame = 0


Query: 41  RTLFPNDVEQ-GAEYMNEKEAMFRRLVVEQLKPVSGLYKVKKWVEDHRLKCAAVTNSSRI 100
           + L P  VE+  A Y     A   +LV    +P  G+Y+  K + +   K A VT   R 
Sbjct: 55  KRLDPERVEEMVATYRKHNHAHHDKLV----EPYEGVYETVKTLHEQGFKLAIVTTKIRE 114

Query: 101 NAELMISMLGLTDFFETIIIGDECEHPKPHPEPYLKALEALQVSKDHTFIFEDS----LT 160
            A   + + GL +FF+ I+  D+ E+ KP+PEP  KA+ AL   K+ T +  D+    L 
Sbjct: 115 TAMKGLKLFGLDEFFDVIVALDDVENVKPNPEPLEKAMNALGAKKEETIMVGDNSHDILG 174

Query: 161 GIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYNDL 195
           G  AGV+    VVG ++R  E  + Q  P  +++   DL
Sbjct: 175 GKNAGVKT--AVVGYAIR-GEDYVRQFDPDYVLRSMPDL 206

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BLAST of CSPI03G02020 vs. ExPASy Swiss-Prot
Match: Q9X0Y1 (Phosphorylated carbohydrates phosphatase TM_1254 OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_1254 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-10
Identity = 44/174 (25.29%), Postives = 86/174 (49.43%), Query Frame = 0


Query: 19  GIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEK-EAMFRRLVVEQLKPVSGLY 78
           G P  E+ + + +     +G+   +   +++   E   ++     +R+  E LK   G+ 
Sbjct: 31  GKPYTEDLHRRIMGVPEREGLPILMEALEIKDSLENFKKRVHEEKKRVFSELLKENPGVR 90

Query: 79  KVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPYLKAL 138
           +  ++V+  R+K A  T++ +  A   +  L L  +F+ ++ GD+ ++ KP PE YL  L
Sbjct: 91  EALEFVKSKRIKLALATSTPQREALERLRRLDLEKYFDVMVFGDQVKNGKPDPEIYLLVL 150

Query: 139 EALQVSKDHTFIFEDSLTGIKAGVEADMPVV--GISMRNSEQLLMQAKPALLVK 190
           E L V  +   +FEDS +G++A   A +  +   +   N  + L++A    LVK
Sbjct: 151 ERLNVVPEKVVVFEDSKSGVEAAKSAGIERIYGVVHSLNDGKALLEAGAVALVK 204

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BLAST of CSPI03G02020 vs. ExPASy Swiss-Prot
Match: Q3J8A0 (Phosphoglycolate phosphatase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107) OX=323261 GN=Noc_2493 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 39/159 (24.53%), Postives = 73/159 (45.91%), Query Frame = 0


Query: 19  GIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAE--YMNEKEAMFRRLVVEQLKPVSGL 78
           G+P+  E  ++   G   + +I+    +++E   E     + E +F  L  +     S L
Sbjct: 35  GLPLRGETKVRQWVGNGVERLIKRALVDNMEGEPEEDLYQKAETIFLALYADNTSKRSHL 94

Query: 79  Y----KVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEP 138
           Y    +   W++    +   VTN +      +++ LG+ D+FE +I GD     KPHP P
Sbjct: 95  YPGVNEGLAWLKSQGYRVGCVTNKAAQFTYPLLTELGIIDYFEIVISGDTLPEKKPHPAP 154

Query: 139 YLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGIS 172
            L A     ++ +   +  DS++ +KA   A+  +V +S
Sbjct: 155 LLHAASHFGIAPEKALMIGDSISDVKAARAANFQIVCLS 193

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BLAST of CSPI03G02020 vs. ExPASy Swiss-Prot
Match: A9VQ75 (Pyrophosphatase PpaX OS=Bacillus mycoides (strain KBAB4) OX=315730 GN=ppaX PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 36/128 (28.12%), Postives = 61/128 (47.66%), Query Frame = 0


Query: 68  EQLKPVSGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPK 127
           E ++    +Y+  + ++    K   VT  +R   E+ + +  L  FF+ ++  D+ EH K
Sbjct: 79  ELVEEYETVYETVQELKKQGYKIGIVTTKARQTVEMGLKLSKLDQFFDVVVTIDDVEHVK 138

Query: 128 PHPEPYLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQA-KPAL 187
           PHPEP  KALE L    + T +  D+   I  G  A    V +S     +  ++A KP  
Sbjct: 139 PHPEPLQKALELLDAKPEETLMVGDNHHDIVGGQNAGTKTVAVSWTLKGRAYLEAYKPDY 198

Query: 188 LVKDYNDL 195
           ++   +DL
Sbjct: 199 VLDKMSDL 206

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BLAST of CSPI03G02020 vs. ExPASy TrEMBL
Match: A0A0A0L202 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016920 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 2.9e-109
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV
Sbjct: 51  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY
Sbjct: 111 SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND
Sbjct: 171 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 230

Query: 194 LKLWLALDELVVRGDTIANA 214
           LKLWLALDELVVRGDTIANA
Sbjct: 231 LKLWLALDELVVRGDTIANA 250

[Close]

BLAST of CSPI03G02020 vs. ExPASy TrEMBL
Match: A0A6J1KXL2 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Cucurbita maxima OX=3661 GN=LOC111498474 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.5e-83
Identity = 155/200 (77.50%), Postives = 175/200 (87.50%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKN+AGK+ND I R LFP+D E+G ++ ++KEAMFRRLV EQLKPV
Sbjct: 51  IGFNGGVPIDEEFFIKNVAGKHNDDIARALFPDDYERGLKFCDDKEAMFRRLVTEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +GLYKVKKW+ED  LK AAVTN+ R NAELMISMLGLTDFFE +IIG ECEH KPHPEPY
Sbjct: 111 NGLYKVKKWIEDCGLKRAAVTNAPRPNAELMISMLGLTDFFEAVIIGGECEHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALEAL+VSKDHTFIFEDS++GIKAGV A+MPVVGI+ RN E LLMQAKPALLVKDY+D
Sbjct: 171 LKALEALKVSKDHTFIFEDSVSGIKAGVAAEMPVVGIATRNPEHLLMQAKPALLVKDYDD 230

Query: 194 LKLWLALDELVVRGDTIANA 214
            KLW ALDEL  RGDTI  A
Sbjct: 231 PKLWAALDELDKRGDTINKA 250

[Close]

BLAST of CSPI03G02020 vs. ExPASy TrEMBL
Match: A0A6J1FED3 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Cucurbita moschata OX=3662 GN=LOC111443283 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 3.0e-82
Identity = 152/200 (76.00%), Postives = 173/200 (86.50%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKN+AGK+ND I R LFP+D E+G ++ ++KEAMFRRLV EQLKPV
Sbjct: 51  IGFNGGVPIDEEFFIKNVAGKHNDDIARALFPDDYERGLKFCDDKEAMFRRLVTEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +GLYKVKKW+EDH LK AAVTN+ R NAELMISMLGL+DFFE +IIG ECEH KPHPEPY
Sbjct: 111 NGLYKVKKWIEDHGLKRAAVTNAPRPNAELMISMLGLSDFFEAVIIGGECEHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L+VSKDHTFIFEDS++GIKAGV A+MPVVGI+ RN E LLMQAKPALLVKDY+D
Sbjct: 171 LKALEVLKVSKDHTFIFEDSISGIKAGVAAEMPVVGIATRNPEHLLMQAKPALLVKDYDD 230

Query: 194 LKLWLALDELVVRGDTIANA 214
            KLW ALDEL  RG  I  A
Sbjct: 231 PKLWAALDELDKRGGNINKA 250

[Close]

BLAST of CSPI03G02020 vs. ExPASy TrEMBL
Match: A0A6J1HKX9 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465097 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.1e-82
Identity = 149/200 (74.50%), Postives = 173/200 (86.50%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKN+AGK+ND + R +FP+D  +G ++ NEKEAMFRRLV EQLKPV
Sbjct: 34  IGFNGGVPIDEEFFIKNVAGKHNDDVARAIFPDDFGRGLKFCNEKEAMFRRLVTEQLKPV 93

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +GLYKVKKW+EDH +K AAVTN+ R NAELMISMLGLTDFFE +IIG ECEH KPHPEPY
Sbjct: 94  NGLYKVKKWIEDHGVKRAAVTNAPRPNAELMISMLGLTDFFEAVIIGGECEHAKPHPEPY 153

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L+V+KDHTFIFEDS +GIKAGV A+MPVVGI+ RN E LLMQAKPAL+VKDY+D
Sbjct: 154 LKALEVLKVTKDHTFIFEDSASGIKAGVAAEMPVVGIATRNPEHLLMQAKPALVVKDYDD 213

Query: 194 LKLWLALDELVVRGDTIANA 214
            +LW ALDEL  RG T+ NA
Sbjct: 214 PRLWAALDELDKRGGTVKNA 233

[Close]

BLAST of CSPI03G02020 vs. ExPASy TrEMBL
Match: A0A6J1HMY1 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465097 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.1e-82
Identity = 149/200 (74.50%), Postives = 173/200 (86.50%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKN+AGK+ND + R +FP+D  +G ++ NEKEAMFRRLV EQLKPV
Sbjct: 51  IGFNGGVPIDEEFFIKNVAGKHNDDVARAIFPDDFGRGLKFCNEKEAMFRRLVTEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +GLYKVKKW+EDH +K AAVTN+ R NAELMISMLGLTDFFE +IIG ECEH KPHPEPY
Sbjct: 111 NGLYKVKKWIEDHGVKRAAVTNAPRPNAELMISMLGLTDFFEAVIIGGECEHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L+V+KDHTFIFEDS +GIKAGV A+MPVVGI+ RN E LLMQAKPAL+VKDY+D
Sbjct: 171 LKALEVLKVTKDHTFIFEDSASGIKAGVAAEMPVVGIATRNPEHLLMQAKPALVVKDYDD 230

Query: 194 LKLWLALDELVVRGDTIANA 214
            +LW ALDEL  RG T+ NA
Sbjct: 231 PRLWAALDELDKRGGTVKNA 250

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BLAST of CSPI03G02020 vs. NCBI nr
Match: XP_004150177.1 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp isoform X1 [Cucumis sativus] >KGN55798.1 hypothetical protein Csa_010342 [Cucumis sativus])

HSP 1 Score: 404.4 bits (1038), Expect = 5.9e-109
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV
Sbjct: 51  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY
Sbjct: 111 SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND
Sbjct: 171 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 230

Query: 194 LKLWLALDELVVRGDTIANA 214
           LKLWLALDELVVRGDTIANA
Sbjct: 231 LKLWLALDELVVRGDTIANA 250

[Close]

BLAST of CSPI03G02020 vs. NCBI nr
Match: XP_031737783.1 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp isoform X2 [Cucumis sativus])

HSP 1 Score: 404.4 bits (1038), Expect = 5.9e-109
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV
Sbjct: 48  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 107

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY
Sbjct: 108 SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 167

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND
Sbjct: 168 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 227

Query: 194 LKLWLALDELVVRGDTIANA 214
           LKLWLALDELVVRGDTIANA
Sbjct: 228 LKLWLALDELVVRGDTIANA 247

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BLAST of CSPI03G02020 vs. NCBI nr
Match: XP_023005510.1 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Cucurbita maxima])

HSP 1 Score: 317.8 bits (813), Expect = 7.3e-83
Identity = 155/200 (77.50%), Postives = 175/200 (87.50%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKN+AGK+ND I R LFP+D E+G ++ ++KEAMFRRLV EQLKPV
Sbjct: 51  IGFNGGVPIDEEFFIKNVAGKHNDDIARALFPDDYERGLKFCDDKEAMFRRLVTEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +GLYKVKKW+ED  LK AAVTN+ R NAELMISMLGLTDFFE +IIG ECEH KPHPEPY
Sbjct: 111 NGLYKVKKWIEDCGLKRAAVTNAPRPNAELMISMLGLTDFFEAVIIGGECEHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALEAL+VSKDHTFIFEDS++GIKAGV A+MPVVGI+ RN E LLMQAKPALLVKDY+D
Sbjct: 171 LKALEALKVSKDHTFIFEDSVSGIKAGVAAEMPVVGIATRNPEHLLMQAKPALLVKDYDD 230

Query: 194 LKLWLALDELVVRGDTIANA 214
            KLW ALDEL  RGDTI  A
Sbjct: 231 PKLWAALDELDKRGDTINKA 250

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BLAST of CSPI03G02020 vs. NCBI nr
Match: XP_023521077.1 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.8 bits (808), Expect = 2.8e-82
Identity = 153/200 (76.50%), Postives = 174/200 (87.00%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKN+AGK+ND I + LFP+D E+G ++ ++KEAMFRRLV EQLKPV
Sbjct: 51  IGFNGGVPIDEEFFIKNVAGKHNDDIAQALFPDDYERGLKFCDDKEAMFRRLVTEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +GLYKVKKW+ED  LK AAVTN+ R NAELMISMLGLTDFFE +IIG ECEH KPHPEPY
Sbjct: 111 NGLYKVKKWIEDRGLKRAAVTNAPRPNAELMISMLGLTDFFEAVIIGGECEHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L+VSKDHTFIFEDS++GIKAGV A+MPVVGI+ RN E LLMQAKPALLVKDY+D
Sbjct: 171 LKALEVLKVSKDHTFIFEDSVSGIKAGVAAEMPVVGIATRNPEHLLMQAKPALLVKDYDD 230

Query: 194 LKLWLALDELVVRGDTIANA 214
           LKLW ALDEL  RG TI  A
Sbjct: 231 LKLWAALDELDKRGGTINKA 250

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BLAST of CSPI03G02020 vs. NCBI nr
Match: XP_038903357.1 (haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Benincasa hispida])

HSP 1 Score: 315.1 bits (806), Expect = 4.7e-82
Identity = 156/200 (78.00%), Postives = 172/200 (86.00%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKNIAGK+ND I R LFP++ E+G ++ +EKEAMFRRLV EQLKPV
Sbjct: 112 IGFNGGVPIDEEFFIKNIAGKHNDDIARALFPDEPERGLKFCDEKEAMFRRLVTEQLKPV 171

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +GLYKVKKW+ED  LK AAVTN+ R NAELMISMLGLTDFFE IIIG ECEH KPHPEPY
Sbjct: 172 NGLYKVKKWIEDRGLKRAAVTNAPRPNAELMISMLGLTDFFEAIIIGGECEHAKPHPEPY 231

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L+VSKDHTFIFEDS++GIKAGV A MPVVGI+ RN E LLMQAKPALLVKDY D
Sbjct: 232 LKALEVLKVSKDHTFIFEDSVSGIKAGVAAGMPVVGITTRNPEHLLMQAKPALLVKDYAD 291

Query: 194 LKLWLALDELVVRGDTIANA 214
            KLW ALDEL  RG TI NA
Sbjct: 292 PKLWAALDELDKRGGTIKNA 311

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BLAST of CSPI03G02020 vs. TAIR 10
Match: AT2G38740.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.6e-67
Identity = 116/190 (61.05%), Postives = 152/190 (80.00%), Query Frame = 0


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFN G+PIDE+F+++NIAGK+N  I   LFP+DV +G ++ +EKEA++R++V E++KP+
Sbjct: 51  IGFNNGVPIDEKFFVENIAGKHNSEIALLLFPDDVSRGLKFCDEKEALYRKIVAEKIKPL 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
            GL K+ KW+ED  LK AAVTN+ + NAELMIS LGLTDFF+ +I+G ECE PKPHP PY
Sbjct: 111 DGLIKLTKWIEDRGLKRAAVTNAPKENAELMISKLGLTDFFQAVILGSECEFPKPHPGPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L VSK+HT +FEDS++GIKAGV A MPV+G++  N   LLMQAKPA L+++Y D
Sbjct: 171 LKALEVLNVSKEHTLVFEDSISGIKAGVAAGMPVIGLTTGNPASLLMQAKPAFLIENYAD 230

Query: 194 LKLWLALDEL 204
            KLW  L+EL
Sbjct: 231 PKLWAVLEEL 240

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BLAST of CSPI03G02020 vs. TAIR 10
Match: AT4G21470.1 (riboflavin kinase/FMN hydrolase )

HSP 1 Score: 54.7 bits (130), Expect = 1.1e-07
Identity = 31/105 (29.52%), Postives = 55/105 (52.38%), Query Frame = 0


Query: 67  VEQLKPVSGLYKVKKWVEDHRLKCAAVTNSSRINAELMISM-LGLTDFFETIIIGDECEH 126
           ++++K + G  ++ + ++ H +  A  +NSSR N E  IS   G  + F  I+  DE   
Sbjct: 89  MDKIKSLPGANRLIRHLKCHGVPVALASNSSRANIESKISYHEGWKECFSVIVGSDEVSK 148

Query: 127 PKPHPEPYLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGI 171
            KP P+ +L+A + L+       + EDS+ G+ AG  A   V+ +
Sbjct: 149 GKPSPDIFLEAAKRLKKDPADCLVIEDSVPGVMAGKAAGTKVIAV 193

The following BLAST results are available for this feature:

BLAST of CSPI03G02020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZVJ5	2.2e-66	61.05	Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Arabidops...	[more]
Q9K6Y7	2.0e-11	32.70	Pyrophosphatase PpaX OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / F...	[more]
Q9X0Y1	1.7e-10	25.29	Phosphorylated carbohydrates phosphatase TM_1254 OS=Thermotoga maritima (strain ...	[more]
Q3J8A0	1.1e-09	24.53	Phosphoglycolate phosphatase OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 1...	[more]
A9VQ75	1.2e-08	28.13	Pyrophosphatase PpaX OS=Bacillus mycoides (strain KBAB4) OX=315730 GN=ppaX PE=3 ...	[more]

BLAST of CSPI03G02020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L202	2.9e-109	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016920 PE=4 SV=1	[more]
A0A6J1KXL2	3.5e-83	77.50	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Cucurbita...	[more]
A0A6J1FED3	3.0e-82	76.00	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Cucurbita...	[more]
A0A6J1HKX9	5.1e-82	74.50	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp isoform X2 O...	[more]
A0A6J1HMY1	5.1e-82	74.50	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp isoform X1 O...	[more]

BLAST of CSPI03G02020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150177.1	5.9e-109	100.00	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp isoform X1 [...	[more]
XP_031737783.1	5.9e-109	100.00	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp isoform X2 [...	[more]
XP_023005510.1	7.3e-83	77.50	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Cucurbita m...	[more]
XP_023521077.1	2.8e-82	76.50	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Cucurbita p...	[more]
XP_038903357.1	4.7e-82	78.00	haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Benincasa h...	[more]

BLAST of CSPI03G02020 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38740.1	1.6e-67	61.05	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT4G21470.1	1.1e-07	29.52	riboflavin kinase/FMN hydrolase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023198	Phosphoglycolate phosphatase-like, domain 2	GENE3D	1.10.150.240	Putative phosphatase; domain 2	coord: 21..68 e-value: 7.6E-42 score: 145.2
IPR041492	Haloacid dehalogenase-like hydrolase	PFAM	PF13419	HAD_2	coord: 27..169 e-value: 8.6E-24 score: 84.6
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 69..194 e-value: 7.6E-42 score: 145.2
IPR006439	HAD hydrolase, subfamily IA	TIGRFAM	TIGR01509	TIGR01509	coord: 90..170 e-value: 2.1E-11 score: 42.2
None	No IPR available	PANTHER	PTHR46193	6-PHOSPHOGLUCONATE PHOSPHATASE	coord: 12..204
None	No IPR available	PANTHER	PTHR46193:SF13	HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN SGPP	coord: 12..204
None	No IPR available	CDD	cd07505	HAD_BPGM-like	coord: 54..170 e-value: 1.21437E-33 score: 115.791
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 19..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G02020.1	CSPI03G02020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity