CSPI03G00050 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G00050
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPectinesterase
LocationChr3: 63935 .. 66859 (+)
RNA-Seq ExpressionCSPI03G00050
SyntenyCSPI03G00050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTTTATTTTATTTAAAACATTTAAAATTGTAGTGTTTACAATGTTGGTTTTGTCCAAATACAATAGAAATGAAAATAAAGGAATAGAATATATTAATGAGGGGGAAGGGATGGATGAAGAGGAAGCGAAAGTCAGGGTGGGAATGGTAGTCAACTTCTATCATTTAATAGCATTTGTAGTTAGGAGAGGAGAGAGTGATATGGGCATTTGTTTTTGGTTCAAAAGAAGAGTAATTAGGAGAGACGAGTATGGGTGTGGATGATGACACTGACATCCAAAAAGATCAAAGGGAGATGTTCGAATCTACTAAAGTTGTCGTAAATGCTTTCCAGCGACTGTGAGTAAGATGCCCCGAGATCCGCGTAAGAAGAGAAGCTTTTTATGAGCGCCGACACATGGATTTTCACACAAACCCTTCCCCACAAAACGTGAATGTAGCATTCAAATCAACGCGCTATCTCCCCAACCCCCTGCCTTTAAAGTTCCTTTCCCTCACCTTCCAACTGGACTCTTCATTTACTTCACCGATTTTTGTTTTAATTTTGCTTCATCATCCCATACATACTGGCCAGGCCAAAGGCAGCTCAATAATTCCTATCTTAATGGCCAAATTAACTACACAAACCCTTCAGTTTTGCTGCCTCTTGATCCTTTTGTTTCCCATGCTGCTTCCGGCCAGCTCTCCACCGGATCTTGTGCAGACAGAGTGCTTGAGCGTACCCTCCTCCCAGTTCTCCAACTCACTCCTCTCCACCATCGATGTTGTACGACAGGTTATGGCCATTTTCTCTCCGTTCAGTAAATTACTGGGAGACTTCCGGCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTTGACTCCTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAGAATCCCAAGGGTACACCATTATTTACAATACCCCACCCCCACCGCATTCTTATAAATTTGCATATTAACTCTCTACCCCCTAAGCCTATTCATTTATATGTTGCGCTTTCAAACTTTGCTTTAATATATGTTGTTGGTTAGTTCACGGGGATATTGTCGGTTGTGCTGCTGCTGGACCTACTAGTTACTTTTATAAATTGCGCATGCAAATGCAACTACACTAGAAATTCAACATATTTACAAATTAAACATCCTCCTGCTTACATTATTTCAGCTAAAAACCATAGCACGGGAGATCTGAGTTCTGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCGGAAACATGCATGGATGGGTTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAGGCGTTAACCAGTTAACTTCACAGCTCTATGACCTTCTTTCCATGGTGAAATCAATCCCCAATCAACCGAGCGAGTTTCCTTCATGGCTGAAATCTGAGGACCAGAACTTGCTGCAAATAAATGACTTGGCCGCCGATGCCACAGTGGCCGCCGACGGGTCTGGGGATTTCACCAATGTGATGGATGCGGTACTAGCAGCCCCTGACAATAGCATACGACGTTATGTCATATACATAAAGAAGGGTGTGTACTTGGAGAACGTGGAGATCAAGAAGAAGAAGTGGAATCTCATGATGATTGGGGATGGAATAGATGCCACCATCATATCGGGTAACCGCAGTTTTATTGACGGTTGGACAACATTCCGATCAGCGACCTTCGGTAATTTTCCCTTTTATTTTAATAATTTCTGAGGACAAAACAAAACGGAGAAATGAAGAAGTGGTTATTAATTAATTTGCAAATGATTTGCAGCTGTAAGTGGAAGAGGATTCATAGCGCGAGACATAACATTCGAGAATACTGCAGGAGCAGAGAAACATCAAGCAGTGGCACTTAGATCGGACTCCGATCTGTCGGTGTTTTTTAGATGTCGAATTAGAGGCTACCAAGACACGCTGTATACGCACACCATGCGCCAATTTTACCGCGAGTGCCAAATCAGTGGGACCGTTGATTTTCTTTTCGGCGATGCCACCGTCGTTTTTCAAAACTGCAGCATCCTGGCGAAGAAGGGTCTTCCCAACCAGAAAAACACCATCACTGCTCAGGGCCGGAAAGACCCAAACCAACCCACCGGTTTCTCCATTCAATTCTGCAACATATCTGCGGATTCAGACCTTAAACCCTCGGTTAACACTACTGCTACGTACCTTGGAAGACCATGGAAGGAATATTCCAGAACCATTATCATGCAATCATACATCAGCGACGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCAATTTTGCATTGAATACACTATTCTACGCTGAGTTTATGAATTACGGACCGGGGGCGGGATTGGCCAAGCGTGTCAATTGGCCCGGTTACCACCGACTGAATCAAACCAGTGAAGCTACTAACTTCACCGTGGCTCAATTCATTGAAGGAAACCTGTGGCTGCCGTCCACGGGTGTCAAATACACTGCAGGACTGGGAGCTAATTAGTAAATCAACAATATGTGGTGCATTGATGCTCCCTCCTATATATCTCACTTTCAAAATATACATAAAAATGAATTAAAACATTAAAAGAAAAAAATGCCTTGCCGTAGTTTAGATGATACAATTCGGTGGACCCAGAAAGTTAAGTGCCTCTAGATATTTTTGGTGTTGTAATTAAAGCAAACCCCCTAAATTCCCCTCGAGTCTTCAGTTCTGTGTGTGCCACCGTGGTGCACTTGCCATATGCTTTTGTGTGACCATTTATCTCTTCACTTTGACCTTCATTTTGTAACATGTTGTTCTAGTGCTTTTGGTCGCAATTGTCCATTGTTTTCTGCCCCTCTTTTCATTACCATCGTATTATTGTTTGATGTTTGTTTAATTGTCATATTGCTCTTCGTCTCTGTCTTTCATCTATTATATATATGTTGTGGTTTTTCTTGC

mRNA sequence

CGTTTATTTTATTTAAAACATTTAAAATTGTAGTGTTTACAATGTTGGTTTTGTCCAAATACAATAGAAATGAAAATAAAGGAATAGAATATATTAATGAGGGGGAAGGGATGGATGAAGAGGAAGCGAAAGTCAGGGTGGGAATGGTAGTCAACTTCTATCATTTAATAGCATTTGTAGTTAGGAGAGGAGAGAGTGATATGGGCATTTGTTTTTGGTTCAAAAGAAGAGTAATTAGGAGAGACGAGTATGGGTGTGGATGATGACACTGACATCCAAAAAGATCAAAGGGAGATGTTCGAATCTACTAAAGTTGTCGTAAATGCTTTCCAGCGACTGTGAGTAAGATGCCCCGAGATCCGCGTAAGAAGAGAAGCTTTTTATGAGCGCCGACACATGGATTTTCACACAAACCCTTCCCCACAAAACGTGAATGTAGCATTCAAATCAACGCGCTATCTCCCCAACCCCCTGCCTTTAAAGTTCCTTTCCCTCACCTTCCAACTGGACTCTTCATTTACTTCACCGATTTTTGTTTTAATTTTGCTTCATCATCCCATACATACTGGCCAGGCCAAAGGCAGCTCAATAATTCCTATCTTAATGGCCAAATTAACTACACAAACCCTTCAGTTTTGCTGCCTCTTGATCCTTTTGTTTCCCATGCTGCTTCCGGCCAGCTCTCCACCGGATCTTGTGCAGACAGAGTGCTTGAGCGTACCCTCCTCCCAGTTCTCCAACTCACTCCTCTCCACCATCGATGTTGTACGACAGGTTATGGCCATTTTCTCTCCGTTCAGTAAATTACTGGGAGACTTCCGGCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTTGACTCCTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAGAATCCCAAGGCTAAAAACCATAGCACGGGAGATCTGAGTTCTGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCGGAAACATGCATGGATGGGTTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAGGCGTTAACCAGTTAACTTCACAGCTCTATGACCTTCTTTCCATGGTGAAATCAATCCCCAATCAACCGAGCGAGTTTCCTTCATGGCTGAAATCTGAGGACCAGAACTTGCTGCAAATAAATGACTTGGCCGCCGATGCCACAGTGGCCGCCGACGGGTCTGGGGATTTCACCAATGTGATGGATGCGGTACTAGCAGCCCCTGACAATAGCATACGACGTTATGTCATATACATAAAGAAGGGTGTGTACTTGGAGAACGTGGAGATCAAGAAGAAGAAGTGGAATCTCATGATGATTGGGGATGGAATAGATGCCACCATCATATCGGGTAACCGCAGTTTTATTGACGGTTGGACAACATTCCGATCAGCGACCTTCGCTGTAAGTGGAAGAGGATTCATAGCGCGAGACATAACATTCGAGAATACTGCAGGAGCAGAGAAACATCAAGCAGTGGCACTTAGATCGGACTCCGATCTGTCGGTGTTTTTTAGATGTCGAATTAGAGGCTACCAAGACACGCTGTATACGCACACCATGCGCCAATTTTACCGCGAGTGCCAAATCAGTGGGACCGTTGATTTTCTTTTCGGCGATGCCACCGTCGTTTTTCAAAACTGCAGCATCCTGGCGAAGAAGGGTCTTCCCAACCAGAAAAACACCATCACTGCTCAGGGCCGGAAAGACCCAAACCAACCCACCGGTTTCTCCATTCAATTCTGCAACATATCTGCGGATTCAGACCTTAAACCCTCGGTTAACACTACTGCTACGTACCTTGGAAGACCATGGAAGGAATATTCCAGAACCATTATCATGCAATCATACATCAGCGACGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCAATTTTGCATTGAATACACTATTCTACGCTGAGTTTATGAATTACGGACCGGGGGCGGGATTGGCCAAGCGTGTCAATTGGCCCGGTTACCACCGACTGAATCAAACCAGTGAAGCTACTAACTTCACCGTGGCTCAATTCATTGAAGGAAACCTGTGGCTGCCGTCCACGGGTGTCAAATACACTGCAGGACTGGGAGCTAATTAGTAAATCAACAATATGTGGTGCATTGATGCTCCCTCCTATATATCTCACTTTCAAAATATACATAAAAATGAATTAAAACATTAAAAGAAAAAAATGCCTTGCCGTAGTTTAGATGATACAATTCGGTGGACCCAGAAAGTTAAGTGCCTCTAGATATTTTTGGTGTTGTAATTAAAGCAAACCCCCTAAATTCCCCTCGAGTCTTCAGTTCTGTGTGTGCCACCGTGGTGCACTTGCCATATGCTTTTGTGTGACCATTTATCTCTTCACTTTGACCTTCATTTTGTAACATGTTGTTCTAGTGCTTTTGGTCGCAATTGTCCATTGTTTTCTGCCCCTCTTTTCATTACCATCGTATTATTGTTTGATGTTTGTTTAATTGTCATATTGCTCTTCGTCTCTGTCTTTCATCTATTATATATATGTTGTGGTTTTTCTTGC

Coding sequence (CDS)

ATGGATTTTCACACAAACCCTTCCCCACAAAACGTGAATGTAGCATTCAAATCAACGCGCTATCTCCCCAACCCCCTGCCTTTAAAGTTCCTTTCCCTCACCTTCCAACTGGACTCTTCATTTACTTCACCGATTTTTGTTTTAATTTTGCTTCATCATCCCATACATACTGGCCAGGCCAAAGGCAGCTCAATAATTCCTATCTTAATGGCCAAATTAACTACACAAACCCTTCAGTTTTGCTGCCTCTTGATCCTTTTGTTTCCCATGCTGCTTCCGGCCAGCTCTCCACCGGATCTTGTGCAGACAGAGTGCTTGAGCGTACCCTCCTCCCAGTTCTCCAACTCACTCCTCTCCACCATCGATGTTGTACGACAGGTTATGGCCATTTTCTCTCCGTTCAGTAAATTACTGGGAGACTTCCGGCTTTCCACCGCCATTTCCGACTGTCTCGATTTGCTTGACTCCTCTGCTGACCAGTTGAGCTGGTCCCTCTCCGCCACCCAGAATCCCAAGGCTAAAAACCATAGCACGGGAGATCTGAGTTCTGATCTGAAGACATGGCTGAGCGCTGCAGTGGTTAATCCGGAAACATGCATGGATGGGTTTGAGGGCACCAATAGCATTATCAAAGGTCTAGTATCTGGAGGCGTTAACCAGTTAACTTCACAGCTCTATGACCTTCTTTCCATGGTGAAATCAATCCCCAATCAACCGAGCGAGTTTCCTTCATGGCTGAAATCTGAGGACCAGAACTTGCTGCAAATAAATGACTTGGCCGCCGATGCCACAGTGGCCGCCGACGGGTCTGGGGATTTCACCAATGTGATGGATGCGGTACTAGCAGCCCCTGACAATAGCATACGACGTTATGTCATATACATAAAGAAGGGTGTGTACTTGGAGAACGTGGAGATCAAGAAGAAGAAGTGGAATCTCATGATGATTGGGGATGGAATAGATGCCACCATCATATCGGGTAACCGCAGTTTTATTGACGGTTGGACAACATTCCGATCAGCGACCTTCGCTGTAAGTGGAAGAGGATTCATAGCGCGAGACATAACATTCGAGAATACTGCAGGAGCAGAGAAACATCAAGCAGTGGCACTTAGATCGGACTCCGATCTGTCGGTGTTTTTTAGATGTCGAATTAGAGGCTACCAAGACACGCTGTATACGCACACCATGCGCCAATTTTACCGCGAGTGCCAAATCAGTGGGACCGTTGATTTTCTTTTCGGCGATGCCACCGTCGTTTTTCAAAACTGCAGCATCCTGGCGAAGAAGGGTCTTCCCAACCAGAAAAACACCATCACTGCTCAGGGCCGGAAAGACCCAAACCAACCCACCGGTTTCTCCATTCAATTCTGCAACATATCTGCGGATTCAGACCTTAAACCCTCGGTTAACACTACTGCTACGTACCTTGGAAGACCATGGAAGGAATATTCCAGAACCATTATCATGCAATCATACATCAGCGACGCCATTAGGCCAGAAGGTTGGCTTGAGTGGAACGCCAATTTTGCATTGAATACACTATTCTACGCTGAGTTTATGAATTACGGACCGGGGGCGGGATTGGCCAAGCGTGTCAATTGGCCCGGTTACCACCGACTGAATCAAACCAGTGAAGCTACTAACTTCACCGTGGCTCAATTCATTGAAGGAAACCTGTGGCTGCCGTCCACGGGTGTCAAATACACTGCAGGACTGGGAGCTAATTAG

Protein sequence

MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPSEFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN*
Homology
[Close]
BLAST of CSPI03G00050 vs. ExPASy Swiss-Prot
Match: Q43062 (Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 1.2e-210
Identity = 363/533 (68.11%), Postives = 436/533 (81.80%), Query Frame = 0 

Query: 46  FVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTEC 105
           ++L+  H+P+  G  K   ++ +L A  ++  + F             + S  D +QT+C
Sbjct: 3   YLLMASHNPLPAG--KQLLLLVLLCAFFSSSFIPFA------------SCSITDDLQTQC 62

Query: 106 LSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSL 165
           L V +++F+ SL  TID V+QV +I S F+   GDFRL+ AISDCLDLLD SAD+L+WSL
Sbjct: 63  LKVSATEFAGSLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADELNWSL 122

Query: 166 SATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQL 225
           SA+QN K KN+STG LSSDL+TWLSAA+VN +TC +GFEGTNSI++GL+S G+ Q+TS +
Sbjct: 123 SASQNQKGKNNSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLV 182

Query: 226 YDLLSMVKSIPNQP---SEFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLA 285
            +LL+ V    NQ     + PSW+K++D+ LLQ + ++ DA VA DG+G+FTNV DAVLA
Sbjct: 183 QELLTQVHPNSNQQGPNGQIPSWVKTKDRKLLQADGVSVDAIVAQDGTGNFTNVTDAVLA 242

Query: 286 APDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSAT 345
           APD S+RRYVIYIK+G Y ENVEIKKKKWNLMMIGDG+DATIISGNRSF+DGWTTFRSAT
Sbjct: 243 APDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSAT 302

Query: 346 FAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYR 405
           FAVSGRGFIARDITFENTAG EKHQAVALRSDSDLSVF+RC IRGYQDTLYTHTMRQFYR
Sbjct: 303 FAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYTHTMRQFYR 362

Query: 406 ECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISA 465
           +C+ISGTVDF+FGDATVVFQNC ILAKKGLPNQKN+ITAQGRKDPN+PTG SIQFCNI+A
Sbjct: 363 DCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGISIQFCNITA 422

Query: 466 DSDLK-PSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFM 525
           DSDL+  SVN+T TYLGRPWK YSRT+IMQS++S+ IRPEGWLEWN +FALN+LFY E+M
Sbjct: 423 DSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALNSLFYGEYM 482

Query: 526 NYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLG 575
           NYGPGAGL  RV WPGY   N++++A N+TVAQFIEGNLWLPSTGVKYTA  G
Sbjct: 483 NYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTAEFG 521

[Close]
BLAST of CSPI03G00050 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 1.3e-193
Identity = 322/498 (64.66%), Postives = 407/498 (81.73%), Query Frame = 0 

Query: 94  ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
           A S  DLVQ ECL VP  +F+ +  + +D + + +AI S F K  G  R+S AI DC+DL
Sbjct: 28  AFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDL 87

Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
           LDS+A++LSW +SA+Q+P  K++STGD+ SDL+TW+SAA+ N +TC+DGFEGTN IIK +
Sbjct: 88  LDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKI 147

Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQP-----------------SEFPSWLKSEDQNLLQI 273
           V+GG++++ + + +LL+MV S P++P                 S+FPSW+K  D+ LLQ 
Sbjct: 148 VAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQT 207

Query: 274 NDL-AADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMM 333
           +++  ADA VAADG+G+FT + DAVLAAPD S +RYVI++K+GVY+ENVEIKKKKWN+MM
Sbjct: 208 DNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMM 267

Query: 334 IGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDS 393
           +GDGIDAT+I+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAG EKHQAVA+RSD+
Sbjct: 268 VGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDT 327

Query: 394 DLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQ 453
           DL VF+RC +RGYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQ
Sbjct: 328 DLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQ 387

Query: 454 KNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYIS 513
           KN+ITAQGRKDPN+PTGF+IQF NI+AD+DL  ++NTTATYLGRPWK YSRT+ MQ+Y+S
Sbjct: 388 KNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMS 447

Query: 514 DAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQF 573
           DAI P GWLEWN NFAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTV+Q 
Sbjct: 448 DAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQL 507

[Close]
BLAST of CSPI03G00050 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 664.5 bits (1713), Expect = 1.1e-189
Identity = 324/521 (62.19%), Postives = 402/521 (77.16%), Query Frame = 0 

Query: 81  CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGD 140
           C    L+  ML    S  +  Q + L VP+S+F +S+ + + V+RQV +I S F+   GD
Sbjct: 3   CLKYFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 62

Query: 141 FRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCM 200
            RL  A+SDCLDLLD S+++L+WS SA++NPK K + TGD+ SD +TWLSAA+ N  TCM
Sbjct: 63  RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 122

Query: 201 DGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMV-------------------KSIP----- 260
           +GF+GT+ ++K LV+G ++QL S L +LL +V                   K+ P     
Sbjct: 123 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 182

Query: 261 ----NQPSEFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYV 320
               ++  +FP W++ +D+ LL+ N    D +VA DG+G+FT +MDA+  APD S  R+V
Sbjct: 183 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 242

Query: 321 IYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIA 380
           IYIKKG+YLENVEIKKKKWN++M+GDGID T+ISGNRSFIDGWTTFRSATFAVSGRGF+A
Sbjct: 243 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLA 302

Query: 381 RDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDF 440
           RDITF+NTAG EKHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF
Sbjct: 303 RDITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDF 362

Query: 441 LFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNT 500
           +FGD TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +NT
Sbjct: 363 IFGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNT 422

Query: 501 TATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKR 560
           T TYLGRPWK YSRT+ +++ +SD +RPEGWLEWNA+FAL+TLFY EFMNYGPG+GL+ R
Sbjct: 423 TRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSR 482

Query: 561 VNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           V WPGYH  N + +A NFTV+QFI+GNLWLPSTGV ++ GL
Sbjct: 483 VKWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523

[Close]
BLAST of CSPI03G00050 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.9e-125
Identity = 231/489 (47.24%), Postives = 318/489 (65.03%), Query Frame = 0 

Query: 99  DLVQTECLSVPSSQFSNSLLSTIDVVRQV---MAIFSPFSKLLGDFRLSTAISDCLDLLD 158
           DL+  E ++  S   S  +   ++ V Q+   + + S     + D R   A++DCL+LLD
Sbjct: 69  DLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQEGALTDCLELLD 128

Query: 159 SSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVS 218
            S D +S S++A        H      ++ ++WLS  + N  TC+D  +   S  K +++
Sbjct: 129 QSVDLVSDSIAAIDKRTHSEH------ANAQSWLSGVLTNHVTCLDELD---SFTKAMIN 188

Query: 219 G-GVNQLTSQLYDLLSMVKSI--PNQP------SEFPSWLKSEDQNLLQIN--DLAADAT 278
           G  +++L S+    L+M+ S+  PN         + PSW+ S D+ L++ +  D+ A+A 
Sbjct: 189 GTNLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGANAV 248

Query: 279 VAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATI 338
           VA DG+G +  + +AV AAPD S  RYVIY+K+G+Y ENVE+  +K  LM++GDG+ ATI
Sbjct: 249 VAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMHATI 308

Query: 339 ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCR 398
           I+GN + +DG TTF SAT A  G+GFI +DI  +NTAG  KHQAVALR  +D SV  RCR
Sbjct: 309 ITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVINRCR 368

Query: 399 IRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGR 458
           I  YQDTLY H+ RQFYR+  ++GT+DF+FG+A VVFQ C ++A+K    Q+N +TAQGR
Sbjct: 369 IDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTAQGR 428

Query: 459 KDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWL 518
            DPNQ TG SIQFCNI A SDL+P +    TYLGRPWK+YSRT++M+SY+   I P GW 
Sbjct: 429 TDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPAGWA 488

Query: 519 EWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPS 574
           EW+ +FAL TL+Y EFMN GPGAG +KRV WPGYH +   +EA  FTVA+ I+G  WL S
Sbjct: 489 EWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSWLRS 548

[Close]
BLAST of CSPI03G00050 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 445.7 bits (1145), Expect = 8.2e-124
Identity = 243/548 (44.34%), Postives = 332/548 (60.58%), Query Frame = 0 

Query: 40  SFTSPIFVLILLHHPIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPD 99
           SF   +FV + L  P    + K S++        T Q  Q C             S   D
Sbjct: 25  SFVITLFVALFLVAPYQV-EIKHSNLCK------TAQDSQLC------------LSYVSD 84

Query: 100 LVQTECLSVPSSQFSNSLLSTIDVVRQV---MAIFSPFSKLLGDFRLSTAISDCLDLLDS 159
           L+  E ++  S   S  +   ++ V Q+   + +       + D R   A++DCL+LLD 
Sbjct: 85  LISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHGALTDCLELLDQ 144

Query: 160 SADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSG 219
           S D  S S++A        H      ++ ++WLS  + N  TC+D  +   S  K +++G
Sbjct: 145 SVDFASDSIAAIDKRSRSEH------ANAQSWLSGVLTNHVTCLDELD---SFTKAMING 204

Query: 220 -GVNQLTSQLYDLLSMVKSIPNQPSE--------FPSWLKSEDQNLLQIN--DLAADATV 279
             + +L S+    L+M+ S+  Q  +         PSW+ S D+ L++ +  D+ A+A V
Sbjct: 205 TNLEELISRAKVALAMLASLTTQDEDVFMTVLGKMPSWVSSMDRKLMESSGKDIIANAVV 264

Query: 280 AADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATII 339
           A DG+GD+  + +AV AAPD S  RYVIY+K+G Y ENVE+   K NLM++GDG+ AT I
Sbjct: 265 AQDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIVGDGMYATTI 324

Query: 340 SGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRI 399
           +G+ + +DG TTFRSAT A  G+GFI +DI  +NTAG  K QAVALR  +D+SV  RCRI
Sbjct: 325 TGSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGADMSVINRCRI 384

Query: 400 RGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRK 459
             YQDTLY H+ RQFYR+  ++GTVDF+FG+A VVFQ C ++A+K    Q+N +TAQGR 
Sbjct: 385 DAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGRT 444

Query: 460 DPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLE 519
           DPNQ TG SIQFCNI A SDL+P +    TYLGRPWKEYSRT++M+SY+   I P GW E
Sbjct: 445 DPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWAE 504

Query: 520 WNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPST 574
           W+ +FAL TL+Y EFMN GPGAG +KRV WPGYH +   ++A  FTVA+ I+G  WL ST
Sbjct: 505 WDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRST 544

[Close]
BLAST of CSPI03G00050 vs. ExPASy TrEMBL
Match: A0A0A0L4S0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1)

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 576/576 (100.00%), Query Frame = 0 

Query: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60
           MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA
Sbjct: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60

Query: 61  KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120
           KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST
Sbjct: 61  KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120

Query: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180
           IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD
Sbjct: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180

Query: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240
           LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS
Sbjct: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240

Query: 241 EFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300
           EFPSWLKSEDQNLLQINDLAADATVAADG+GDFTNVMDAVLAAPDNSIRRYVIYIKKGVY
Sbjct: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300

Query: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360
           LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT
Sbjct: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360

Query: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420
           AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV
Sbjct: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420

Query: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480
           FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP
Sbjct: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480

Query: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540
           WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR
Sbjct: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540

Query: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN
Sbjct: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576

[Close]
BLAST of CSPI03G00050 vs. ExPASy TrEMBL
Match: A0A1S3BKA0 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490630 PE=3 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 6.6e-302
Identity = 530/578 (91.70%), Postives = 539/578 (93.25%), Query Frame = 0 

Query: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLK--FLSLTFQLDSSFTSPIFVLILLHHPIHTG 60
           MDF   P P     + +     P P   K  F  L   L   FT   FVLILLHHPIHTG
Sbjct: 1   MDFSDKPFPTKRECSIQINALSPQPPAFKVPFPHLPTGLFIYFTD--FVLILLHHPIHTG 60

Query: 61  QAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLL 120
           Q KGSSIIPILMAKLTTQ LQFCC+ ILLFPMLLPASSP DLVQTECLSVPSSQFSNSLL
Sbjct: 61  QGKGSSIIPILMAKLTTQILQFCCIFILLFPMLLPASSPADLVQTECLSVPSSQFSNSLL 120

Query: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHST 180
           STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAK+HST
Sbjct: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHST 180

Query: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQ 240
           GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG+NQLTSQLYDLLSMVKSIPNQ
Sbjct: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQ 240

Query: 241 PSEFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKG 300
           PSEFPSWLKSED+NLLQ+NDLAADATVAADG+GDFTNVMDAVLAAPD S RRYVIYIKKG
Sbjct: 241 PSEFPSWLKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKG 300

Query: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360
           VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE
Sbjct: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360

Query: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420
           NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT
Sbjct: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420

Query: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480
           VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG
Sbjct: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480

Query: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540
           RPWKEYSRTIIMQSYISDAIRPEGWLEWN NFALNTLFYAEFMNYGPGAGLAKRVNWPGY
Sbjct: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540

Query: 541 HRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           HRLNQTSEATNFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 541 HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 576

[Close]
BLAST of CSPI03G00050 vs. ExPASy TrEMBL
Match: A0A5D3CHH9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00030 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.5e-277
Identity = 477/487 (97.95%), Postives = 483/487 (99.18%), Query Frame = 0 

Query: 90  MLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISD 149
           MLLPASSP DLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISD
Sbjct: 1   MLLPASSPADLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISD 60

Query: 150 CLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSI 209
           CLDLLDSSADQLSWSLSATQNPKAK+HSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSI
Sbjct: 61  CLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSI 120

Query: 210 IKGLVSGGVNQLTSQLYDLLSMVKSIPNQPSEFPSWLKSEDQNLLQINDLAADATVAADG 269
           IKGLVSGG+NQLTSQLYDLLSMVKSIPNQPSEFPSWLKSED+NLLQ+NDLAADATVAADG
Sbjct: 121 IKGLVSGGINQLTSQLYDLLSMVKSIPNQPSEFPSWLKSEDRNLLQLNDLAADATVAADG 180

Query: 270 SGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNR 329
           +GDFTNVMDAVLAAPD S RRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNR
Sbjct: 181 TGDFTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNR 240

Query: 330 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQ 389
           SFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQ
Sbjct: 241 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQ 300

Query: 390 DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ 449
           DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ
Sbjct: 301 DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ 360

Query: 450 PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNAN 509
           PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWN N
Sbjct: 361 PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNGN 420

Query: 510 FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKY 569
           FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGN+WLPSTGVKY
Sbjct: 421 FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKY 480

Query: 570 TAGLGAN 577
           TAGLGAN
Sbjct: 481 TAGLGAN 487

[Close]
BLAST of CSPI03G00050 vs. ExPASy TrEMBL
Match: A0A6J1I863 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470062 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.9e-257
Identity = 466/589 (79.12%), Postives = 507/589 (86.08%), Query Frame = 0 

Query: 9   PQNV----NVAFKSTRYLPNPLPLKFL-SLTF----------QLDSSFTSPIFVLILLHH 68
           P NV     V FKSTR+LPN     FL SLTF           + S  TSP+   +    
Sbjct: 16  PHNVIVKATVPFKSTRHLPNLRLRPFLNSLTFPPKYFIYSDSPVSSPVTSPVSSPV---S 75

Query: 69  PIHTGQAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQF 128
            ++T   +G    PILMAKLT   L+F   ++L+   L PA+   DL QTECL VPSS+F
Sbjct: 76  SLNTNHKQGHLNNPILMAKLTKTHLKFWLFMLLILFPLAPAA---DLEQTECLKVPSSEF 135

Query: 129 SNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKA 188
           SNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA QNPKA
Sbjct: 136 SNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKA 195

Query: 189 KNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVK 248
           KN+STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G+NQLTSQ+Y+LLSMVK
Sbjct: 196 KNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVK 255

Query: 249 SIPNQP-----SEFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSI 308
           SI NQP     S FP+WLKSE+Q+LLQ ND AADATVAADG+GDFT VM+AVLAAPD S+
Sbjct: 256 SISNQPAGKGESGFPAWLKSEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSM 315

Query: 309 RRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSG- 368
           RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATFAVSG 
Sbjct: 316 RRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGR 375

Query: 369 RGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQIS 428
           RGFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTLYTHTMRQFYREC IS
Sbjct: 376 RGFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHIS 435

Query: 429 GTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLK 488
           GTVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+
Sbjct: 436 GTVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQ 495

Query: 489 PSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGA 548
           PSVNTTATYLGRPWKEYSRTIIMQSY+S+AIRPEGWLEWN +FALNTLFYAEFMNYGPGA
Sbjct: 496 PSVNTTATYLGRPWKEYSRTIIMQSYMSNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGA 555

Query: 549 GLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           GLAKR+NWPGYHRLNQTSEATNFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 556 GLAKRINWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 598

[Close]
BLAST of CSPI03G00050 vs. ExPASy TrEMBL
Match: A0A6J1GCJ9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452924 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 6.7e-254
Identity = 443/513 (86.35%), Postives = 477/513 (92.98%), Query Frame = 0 

Query: 70  MAKLTTQTLQF-CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVM 129
           MAKLT   L+F   +L++LFPM  PA+   DL QTECL VPSS+FSNS+LSTI VVRQVM
Sbjct: 1   MAKLTKTHLKFWLFMLLILFPM-APAA---DLEQTECLKVPSSEFSNSVLSTIGVVRQVM 60

Query: 130 AIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTW 189
            IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA QNPKAKN+STGDLSSDLKTW
Sbjct: 61  LIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKAKNNSTGDLSSDLKTW 120

Query: 190 LSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQP-----SEFP 249
           LSAA VNPETCM+GFEGTNSI+KGLVS G+NQLTSQ+Y+LLSMVKSI NQP     SEFP
Sbjct: 121 LSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVKSISNQPVGKGESEFP 180

Query: 250 SWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLEN 309
           +WLK+E+Q+LLQ ND AADATVAADG+GDFT VM+AVLAAPD S+RRYVIY+KKGVY EN
Sbjct: 181 AWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSMRRYVIYVKKGVYFEN 240

Query: 310 VEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGA 369
           VEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAG 
Sbjct: 241 VEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGP 300

Query: 370 EKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQN 429
           EKHQAVALRSDSDLSVFFRCR+RGYQDTLYTHTMRQFYREC ISGTVDFLFGDATVVFQN
Sbjct: 301 EKHQAVALRSDSDLSVFFRCRMRGYQDTLYTHTMRQFYRECHISGTVDFLFGDATVVFQN 360

Query: 430 CSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKE 489
           C+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+PSVN TATYLGRPWKE
Sbjct: 361 CTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQPSVNATATYLGRPWKE 420

Query: 490 YSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ 549
           YSRTIIMQSY+S+AIRPEGWLEWN +FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ
Sbjct: 421 YSRTIIMQSYVSNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQ 480

Query: 550 TSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           TSEATNFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 481 TSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 509

[Close]
BLAST of CSPI03G00050 vs. NCBI nr
Match: XP_004146188.2 (pectinesterase/pectinesterase inhibitor PPE8B [Cucumis sativus] >XP_031738323.1 pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis sativus] >KGN55582.1 hypothetical protein Csa_011581 [Cucumis sativus])

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 576/576 (100.00%), Query Frame = 0 

Query: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60
           MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA
Sbjct: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLKFLSLTFQLDSSFTSPIFVLILLHHPIHTGQA 60

Query: 61  KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120
           KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST
Sbjct: 61  KGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLST 120

Query: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180
           IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD
Sbjct: 121 IDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGD 180

Query: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240
           LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS
Sbjct: 181 LSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQPS 240

Query: 241 EFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300
           EFPSWLKSEDQNLLQINDLAADATVAADG+GDFTNVMDAVLAAPDNSIRRYVIYIKKGVY
Sbjct: 241 EFPSWLKSEDQNLLQINDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVY 300

Query: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360
           LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT
Sbjct: 301 LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENT 360

Query: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420
           AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV
Sbjct: 361 AGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVV 420

Query: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480
           FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP
Sbjct: 421 FQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRP 480

Query: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540
           WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR
Sbjct: 481 WKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHR 540

Query: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN
Sbjct: 541 LNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 576

[Close]
BLAST of CSPI03G00050 vs. NCBI nr
Match: XP_008448427.1 (PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo])

HSP 1 Score: 1045.8 bits (2703), Expect = 1.4e-301
Identity = 530/578 (91.70%), Postives = 539/578 (93.25%), Query Frame = 0 

Query: 1   MDFHTNPSPQNVNVAFKSTRYLPNPLPLK--FLSLTFQLDSSFTSPIFVLILLHHPIHTG 60
           MDF   P P     + +     P P   K  F  L   L   FT   FVLILLHHPIHTG
Sbjct: 1   MDFSDKPFPTKRECSIQINALSPQPPAFKVPFPHLPTGLFIYFTD--FVLILLHHPIHTG 60

Query: 61  QAKGSSIIPILMAKLTTQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLL 120
           Q KGSSIIPILMAKLTTQ LQFCC+ ILLFPMLLPASSP DLVQTECLSVPSSQFSNSLL
Sbjct: 61  QGKGSSIIPILMAKLTTQILQFCCIFILLFPMLLPASSPADLVQTECLSVPSSQFSNSLL 120

Query: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHST 180
           STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAK+HST
Sbjct: 121 STIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKDHST 180

Query: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIPNQ 240
           GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGG+NQLTSQLYDLLSMVKSIPNQ
Sbjct: 181 GDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGINQLTSQLYDLLSMVKSIPNQ 240

Query: 241 PSEFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKG 300
           PSEFPSWLKSED+NLLQ+NDLAADATVAADG+GDFTNVMDAVLAAPD S RRYVIYIKKG
Sbjct: 241 PSEFPSWLKSEDRNLLQLNDLAADATVAADGTGDFTNVMDAVLAAPDKSTRRYVIYIKKG 300

Query: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360
           VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE
Sbjct: 301 VYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFE 360

Query: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420
           NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT
Sbjct: 361 NTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDAT 420

Query: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480
           VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG
Sbjct: 421 VVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLG 480

Query: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540
           RPWKEYSRTIIMQSYISDAIRPEGWLEWN NFALNTLFYAEFMNYGPGAGLAKRVNWPGY
Sbjct: 481 RPWKEYSRTIIMQSYISDAIRPEGWLEWNGNFALNTLFYAEFMNYGPGAGLAKRVNWPGY 540

Query: 541 HRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           HRLNQTSEATNFTVAQFIEGN+WLPSTGVKYTAGLGAN
Sbjct: 541 HRLNQTSEATNFTVAQFIEGNMWLPSTGVKYTAGLGAN 576

[Close]
BLAST of CSPI03G00050 vs. NCBI nr
Match: KAA0052899.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo var. makuwa] >TYK11353.1 pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo var. makuwa])

HSP 1 Score: 964.1 bits (2491), Expect = 5.2e-277
Identity = 477/487 (97.95%), Postives = 483/487 (99.18%), Query Frame = 0 

Query: 90  MLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISD 149
           MLLPASSP DLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISD
Sbjct: 1   MLLPASSPADLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISD 60

Query: 150 CLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSI 209
           CLDLLDSSADQLSWSLSATQNPKAK+HSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSI
Sbjct: 61  CLDLLDSSADQLSWSLSATQNPKAKDHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSI 120

Query: 210 IKGLVSGGVNQLTSQLYDLLSMVKSIPNQPSEFPSWLKSEDQNLLQINDLAADATVAADG 269
           IKGLVSGG+NQLTSQLYDLLSMVKSIPNQPSEFPSWLKSED+NLLQ+NDLAADATVAADG
Sbjct: 121 IKGLVSGGINQLTSQLYDLLSMVKSIPNQPSEFPSWLKSEDRNLLQLNDLAADATVAADG 180

Query: 270 SGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNR 329
           +GDFTNVMDAVLAAPD S RRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNR
Sbjct: 181 TGDFTNVMDAVLAAPDKSTRRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNR 240

Query: 330 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQ 389
           SFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQ
Sbjct: 241 SFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQ 300

Query: 390 DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ 449
           DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ
Sbjct: 301 DTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQ 360

Query: 450 PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNAN 509
           PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWN N
Sbjct: 361 PTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNGN 420

Query: 510 FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKY 569
           FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGN+WLPSTGVKY
Sbjct: 421 FALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNMWLPSTGVKY 480

Query: 570 TAGLGAN 577
           TAGLGAN
Sbjct: 481 TAGLGAN 487

[Close]
BLAST of CSPI03G00050 vs. NCBI nr
Match: XP_038905308.1 (pectinesterase/pectinesterase inhibitor PPE8B-like [Benincasa hispida])

HSP 1 Score: 917.1 bits (2369), Expect = 7.3e-263
Identity = 457/509 (89.78%), Postives = 483/509 (94.89%), Query Frame = 0 

Query: 70  MAKLTTQTLQFCC-LLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVM 129
           MAKLTT+ LQFCC   ILLFP +LPA+S  DLVQTECL VP SQFSNSLLSTID VRQVM
Sbjct: 1   MAKLTTKPLQFCCFFFILLFP-ILPATSSVDLVQTECLKVPFSQFSNSLLSTIDGVRQVM 60

Query: 130 AIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTW 189
            IFSPF+KLLGD RLSTAISDCLDLLDSS DQLSWSLSATQNPKAK++STGDLSSDLKTW
Sbjct: 61  TIFSPFTKLLGDLRLSTAISDCLDLLDSSVDQLSWSLSATQNPKAKDYSTGDLSSDLKTW 120

Query: 190 LSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIP-NQPSEFPSWLK 249
           LSAAVVNPETC+DGFEGTNS IKGLVSGG++QLTSQLYDLLSMVKS P NQP+EFPSW+K
Sbjct: 121 LSAAVVNPETCIDGFEGTNSFIKGLVSGGIDQLTSQLYDLLSMVKSFPNNQPAEFPSWVK 180

Query: 250 SEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIK 309
           SED+NLL +NDLAADATVAADG+GDFT+VMDAV AAPDNSI+RYVIYIK+GVYLENVEIK
Sbjct: 181 SEDRNLLHLNDLAADATVAADGTGDFTSVMDAVRAAPDNSIQRYVIYIKRGVYLENVEIK 240

Query: 310 KKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQ 369
           KKKWNLMMIGDGIDATIISG R+F+DGWTTFRSATFAVSGRGFIARDITFENTAG EKHQ
Sbjct: 241 KKKWNLMMIGDGIDATIISGKRNFVDGWTTFRSATFAVSGRGFIARDITFENTAGPEKHQ 300

Query: 370 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSIL 429
           AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGD TVVFQNC+IL
Sbjct: 301 AVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDGTVVFQNCTIL 360

Query: 430 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRT 489
           AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+P VNTTATYLGRPWKEYSRT
Sbjct: 361 AKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLEPLVNTTATYLGRPWKEYSRT 420

Query: 490 IIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEA 549
           IIMQSY+SDAIRPEGWLEWN +FALNTLFYAEFMN+GPGA LAKRVNWPGY RLN TSEA
Sbjct: 421 IIMQSYMSDAIRPEGWLEWNGDFALNTLFYAEFMNHGPGARLAKRVNWPGYRRLNHTSEA 480

Query: 550 TNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
           TNFTVAQFIEGN+WLPSTGVK+TAGLGAN
Sbjct: 481 TNFTVAQFIEGNMWLPSTGVKFTAGLGAN 508

[Close]
BLAST of CSPI03G00050 vs. NCBI nr
Match: KAG6596294.1 (Histone acetyltransferase HAC1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902.9 bits (2332), Expect = 1.4e-258
Identity = 470/588 (79.93%), Postives = 510/588 (86.73%), Query Frame = 0 

Query: 9    PQNV----NVAFKSTRYLPNPLPLKFL-SLTF------QLDSSFTSPIFVLILLHHPI-- 68
            P NV     V FKSTR+LPN     FL SLTF        DS  +SP+    +   P+  
Sbjct: 3115 PHNVIVKATVPFKSTRHLPNLRLRPFLNSLTFPPKSFIYSDSPVSSPVSSSPVSSSPVSS 3174

Query: 69   -HTGQAKGSSIIPILMAKLTTQTLQF-CCLLILLFPMLLPASSPPDLVQTECLSVPSSQF 128
             +T   +     PILMAKLT   L+F   +L++LFPM  PA+   DL QTECL VPSS+F
Sbjct: 3175 LNTNHKQSHLNNPILMAKLTKTHLKFWMFMLLILFPM-APAA---DLEQTECLKVPSSEF 3234

Query: 129  SNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKA 188
            SNS+LSTI VVRQVM IFSPF+K LGD RLSTAISDCLDLLDSS+DQLSWSLSA QNPKA
Sbjct: 3235 SNSVLSTIGVVRQVMLIFSPFTKFLGDSRLSTAISDCLDLLDSSSDQLSWSLSAAQNPKA 3294

Query: 189  KNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVK 248
            KN+STGDLSSDLKTWLSAA VNPETCM+GFEGTNSI+KGLVS G+NQLTSQ+Y+LLSMVK
Sbjct: 3295 KNNSTGDLSSDLKTWLSAAGVNPETCMEGFEGTNSIVKGLVSEGINQLTSQVYNLLSMVK 3354

Query: 249  SIPNQP-----SEFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSI 308
            SI NQP     SEFP+WLK+E+Q+LLQ ND AADATVAADG+GDFT VM+AVLAAPD S+
Sbjct: 3355 SISNQPAGKGESEFPAWLKTEEQSLLQQNDFAADATVAADGTGDFTTVMEAVLAAPDKSM 3414

Query: 309  RRYVIYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGR 368
            RRYVIY+KKGVY ENVEIKKKKWN+MMIGDGIDAT+ISGNRSFIDGWTTFRSATFAVSGR
Sbjct: 3415 RRYVIYVKKGVYFENVEIKKKKWNIMMIGDGIDATVISGNRSFIDGWTTFRSATFAVSGR 3474

Query: 369  GFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISG 428
            GFIARDITFENTAG EKHQAVALRSDSDLSVFFRCR+ GYQDTLYTHTMRQFYREC ISG
Sbjct: 3475 GFIARDITFENTAGPEKHQAVALRSDSDLSVFFRCRMSGYQDTLYTHTMRQFYRECHISG 3534

Query: 429  TVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKP 488
            TVDFLFGDATVVFQNC+ILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDL+P
Sbjct: 3535 TVDFLFGDATVVFQNCTILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLQP 3594

Query: 489  SVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAG 548
            SVN TATYLGRPWKEYSRTIIMQSYIS+AIRPEGWLEWN +FALNTLFYAEFMNYGPGAG
Sbjct: 3595 SVNATATYLGRPWKEYSRTIIMQSYISNAIRPEGWLEWNGDFALNTLFYAEFMNYGPGAG 3654

Query: 549  LAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGLGAN 577
            LAKRVNWPGYHRLNQTSEATNFTV+QFIEGNLWLPSTGVK+T+G GAN
Sbjct: 3655 LAKRVNWPGYHRLNQTSEATNFTVSQFIEGNLWLPSTGVKFTSGFGAN 3698

[Close]
BLAST of CSPI03G00050 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 677.6 bits (1747), Expect = 9.1e-195
Identity = 322/498 (64.66%), Postives = 407/498 (81.73%), Query Frame = 0 

Query: 94  ASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDFRLSTAISDCLDL 153
           A S  DLVQ ECL VP  +F+ +  + +D + + +AI S F K  G  R+S AI DC+DL
Sbjct: 28  AFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDL 87

Query: 154 LDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMDGFEGTNSIIKGL 213
           LDS+A++LSW +SA+Q+P  K++STGD+ SDL+TW+SAA+ N +TC+DGFEGTN IIK +
Sbjct: 88  LDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFEGTNGIIKKI 147

Query: 214 VSGGVNQLTSQLYDLLSMVKSIPNQP-----------------SEFPSWLKSEDQNLLQI 273
           V+GG++++ + + +LL+MV S P++P                 S+FPSW+K  D+ LLQ 
Sbjct: 148 VAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQT 207

Query: 274 NDL-AADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMM 333
           +++  ADA VAADG+G+FT + DAVLAAPD S +RYVI++K+GVY+ENVEIKKKKWN+MM
Sbjct: 208 DNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMM 267

Query: 334 IGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDS 393
           +GDGIDAT+I+GNRSFIDGWTTFRSATFAVSGRGFIARDITF+NTAG EKHQAVA+RSD+
Sbjct: 268 VGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDT 327

Query: 394 DLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQ 453
           DL VF+RC +RGYQDTLY H+MRQF+REC I+GTVDF+FGDAT VFQ+C I AK+GLPNQ
Sbjct: 328 DLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQ 387

Query: 454 KNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYIS 513
           KN+ITAQGRKDPN+PTGF+IQF NI+AD+DL  ++NTTATYLGRPWK YSRT+ MQ+Y+S
Sbjct: 388 KNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMS 447

Query: 514 DAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQF 573
           DAI P GWLEWN NFAL+TL+Y E+MN GPGA L +RV WPGYH LN ++EA NFTV+Q 
Sbjct: 448 DAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQL 507

[Close]
BLAST of CSPI03G00050 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 664.5 bits (1713), Expect = 8.0e-191
Identity = 324/521 (62.19%), Postives = 402/521 (77.16%), Query Frame = 0 

Query: 81  CCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGD 140
           C    L+  ML    S  +  Q + L VP+S+F +S+ + + V+RQV +I S F+   GD
Sbjct: 3   CLKYFLILLMLGLCVSSEENFQYDYLKVPASEFVSSINTIVVVIRQVSSILSQFADFSGD 62

Query: 141 FRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCM 200
            RL  A+SDCLDLLD S+++L+WS SA++NPK K + TGD+ SD +TWLSAA+ N  TCM
Sbjct: 63  RRLQNAVSDCLDLLDFSSEELTWSASASENPKGKGNGTGDVGSDTRTWLSAALSNQATCM 122

Query: 201 DGFEGTNSIIKGLVSGGVNQLTSQLYDLLSMV-------------------KSIP----- 260
           +GF+GT+ ++K LV+G ++QL S L +LL +V                   K+ P     
Sbjct: 123 EGFDGTSGLVKSLVAGSLDQLYSMLRELLPLVQPEQKPKAVSKPGPIAKGPKAPPGRKLR 182

Query: 261 ----NQPSEFPSWLKSEDQNLLQINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYV 320
               ++  +FP W++ +D+ LL+ N    D +VA DG+G+FT +MDA+  APD S  R+V
Sbjct: 183 DTDEDESLQFPDWVRPDDRKLLESNGRTYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFV 242

Query: 321 IYIKKGVYLENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIA 380
           IYIKKG+YLENVEIKKKKWN++M+GDGID T+ISGNRSFIDGWTTFRSATFAVSGRGF+A
Sbjct: 243 IYIKKGLYLENVEIKKKKWNIVMLGDGIDVTVISGNRSFIDGWTTFRSATFAVSGRGFLA 302

Query: 381 RDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDF 440
           RDITF+NTAG EKHQAVALRSDSDLSVFFRC +RGYQDTLYTHTMRQFYREC I+GTVDF
Sbjct: 303 RDITFQNTAGPEKHQAVALRSDSDLSVFFRCAMRGYQDTLYTHTMRQFYRECTITGTVDF 362

Query: 441 LFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNT 500
           +FGD TVVFQNC ILAK+GLPNQKNTITAQGRKD NQP+GFSIQF NISAD+DL P +NT
Sbjct: 363 IFGDGTVVFQNCQILAKRGLPNQKNTITAQGRKDVNQPSGFSIQFSNISADADLVPYLNT 422

Query: 501 TATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKR 560
           T TYLGRPWK YSRT+ +++ +SD +RPEGWLEWNA+FAL+TLFY EFMNYGPG+GL+ R
Sbjct: 423 TRTYLGRPWKLYSRTVFIRNNMSDVVRPEGWLEWNADFALDTLFYGEFMNYGPGSGLSSR 482

Query: 561 VNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           V WPGYH  N + +A NFTV+QFI+GNLWLPSTGV ++ GL
Sbjct: 483 VKWPGYHVFNNSDQANNFTVSQFIKGNLWLPSTGVTFSDGL 523

[Close]
BLAST of CSPI03G00050 vs. TAIR 10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 437.2 bits (1123), Expect = 2.1e-122
Identity = 221/452 (48.89%), Postives = 296/452 (65.49%), Query Frame = 0 

Query: 142 RLSTAISDCLDLLDSSADQLSWSLSATQNPKA--------KNHSTGDLSSDLKTWLSAAV 201
           R   AI DC +L+  S  +L+WS+                 +H       +LKTWLSAA+
Sbjct: 100 REQVAIEDCKELVGFSVTELAWSMLEMNKLHGGGGIDLDDGSHDAAAAGGNLKTWLSAAM 159

Query: 202 VNPETCMDGFEGTNSIIKGLVSGG---VNQLTSQLYDLLSMVKSIPNQPSE------FPS 261
            N +TC++GFEGT    + L+ G    V QL S + D+ + + ++P + S        P 
Sbjct: 160 SNQDTCLEGFEGTERKYEELIKGSLRQVTQLVSNVLDMYTQLNALPFKASRNESVIASPE 219

Query: 262 WLKSEDQNLLQIND---LAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYL 321
           WL   D++L+  +D   +  +  VA DG G +  + +A+  AP++S +RYVIY+KKGVY 
Sbjct: 220 WLTETDESLMMRHDPSVMHPNTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYK 279

Query: 322 ENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTA 381
           EN+++KKKK N+M++GDGI  TII+G+R+F+ G TTFR+AT AVSGRGFIA+DITF NTA
Sbjct: 280 ENIDLKKKKTNIMLVGDGIGQTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTA 339

Query: 382 GAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVF 441
           G +  QAVALR DSD S F+RC + GYQDTLY H++RQFYR+C+I GT+DF+FG+   V 
Sbjct: 340 GPQNRQAVALRVDSDQSAFYRCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVL 399

Query: 442 QNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPW 501
           QNC I  +  LP QK TITAQGRK PNQ TGF IQ   + A         T  TYLGRPW
Sbjct: 400 QNCKIYTRVPLPLQKVTITAQGRKSPNQNTGFVIQNSYVLA---------TQPTYLGRPW 459

Query: 502 KEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRL 561
           K YSRT+ M +Y+S  ++P GWLEW  NFAL+TL+Y E+ N GPG   + RV WPGYH +
Sbjct: 460 KLYSRTVYMNTYMSQLVQPRGWLEWFGNFALDTLWYGEYNNIGPGWRSSGRVKWPGYHIM 519

Query: 562 NQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           ++ + A +FTV  FI+G  WLP+TGV +TAGL
Sbjct: 520 DKRT-ALSFTVGSFIDGRRWLPATGVTFTAGL 541

[Close]
BLAST of CSPI03G00050 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 429.5 bits (1103), Expect = 4.3e-120
Identity = 236/529 (44.61%), Postives = 324/529 (61.25%), Query Frame = 0 

Query: 75  TQTLQFCCLLILLFPMLLPASSPPDLVQTECLSVPSSQFSNSLLSTIDVVRQVMAIFSPF 134
           T+  + C   ++ FP  L ASS  DL+    +++    FS++L S+  +    M      
Sbjct: 94  TRFPELCVDSLMDFPGSLAASSSKDLIHV-TVNMTLHHFSHALYSSASLSFVDMPP---- 153

Query: 135 SKLLGDFRLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVV 194
                  R  +A   C++LLD S D LS +LS+  +  AK         D+ TWLSAA+ 
Sbjct: 154 -------RARSAYDSCVELLDDSVDALSRALSSVVSSSAK-------PQDVTTWLSAALT 213

Query: 195 NPETCMDGFEGTNSIIKGLVSGGV-NQLTSQLYDLLSMVKS------------------- 254
           N +TC +GF+G +        GGV + +T+ L +L  +V +                   
Sbjct: 214 NHDTCTEGFDGVD-------DGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPI 273

Query: 255 -------IPNQPSEFPSWLKSEDQNLLQ--INDLAADATVAADGSGDFTNVMDAVLAAPD 314
                  +  +  +FP W++ +++ +L+  ++ + AD  V+ DG+G    + +A+  AP 
Sbjct: 274 QNRRLLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQ 333

Query: 315 NSIRRYVIYIKKGVYLE-NVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFA 374
           NS RR +IY+K G Y E N+++ +KK NLM +GDG   T+ISG +S  D  TTF +A+FA
Sbjct: 334 NSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFA 393

Query: 375 VSGRGFIARDITFENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYREC 434
            +G GFIARDITFEN AG  KHQAVALR  +D +V +RC I GYQDTLY H+ RQF+REC
Sbjct: 394 ATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFREC 453

Query: 435 QISGTVDFLFGDATVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADS 494
            I GTVDF+FG+A VV QNCSI A+K +  QKNTITAQ RKDPNQ TG SI    + A S
Sbjct: 454 DIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAAS 513

Query: 495 DLKPSVNTTATYLGRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYG 554
           DL+ +  +T TYLGRPWK +SRT+ M SYI   +   GWLEWN  FAL+TL+Y E++N G
Sbjct: 514 DLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSG 573

Query: 555 PGAGLAKRVNWPGYHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           PG+GL +RV+WPGY  +N T+EA  FTVA+FI G+ WLPSTGV + AGL
Sbjct: 574 PGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596

[Close]
BLAST of CSPI03G00050 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 428.3 bits (1100), Expect = 9.6e-120
Identity = 227/456 (49.78%), Postives = 296/456 (64.91%), Query Frame = 0 

Query: 142 RLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLS-SDLKTWLSAAVVNPETCM 201
           R+ +A   CL+LLD S D L+ +LS+          +GD S SD+ TWLS+A+ N +TC 
Sbjct: 136 RVRSAYDSCLELLDDSVDALTRALSSVV------VVSGDESHSDVMTWLSSAMTNHDTCT 195

Query: 202 DGF---EGTNSIIKGLVSGGVNQLTSQLYDLLSM----VKSIPNQP-------------S 261
           DGF   EG    +K  V G V  L+  + + L++    VK +   P              
Sbjct: 196 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETE 255

Query: 262 EFPSWLKSEDQNLL--QINDLAADATVAADGSGDFTNVMDAVLAAPDNSIRRYVIYIKKG 321
           E P+WLK ED+ LL    + + AD TV+ DGSG F  + +A+  AP++S RR+VIY+K G
Sbjct: 256 ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAG 315

Query: 322 VY-LENVEIKKKKWNLMMIGDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITF 381
            Y  EN+++ +KK NLM IGDG   T+I+G +S  D  TTF +ATFA +G GFI RD+TF
Sbjct: 316 RYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTF 375

Query: 382 ENTAGAEKHQAVALRSDSDLSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDA 441
           EN AG  KHQAVALR   D +V +RC I GYQD LY H+ RQF+REC+I GTVDF+FG+A
Sbjct: 376 ENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNA 435

Query: 442 TVVFQNCSILAKKGLPNQKNTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYL 501
            V+ Q+C+I A+K +  QK TITAQ RKDPNQ TG SI  C + A  DL+ S  +  TYL
Sbjct: 436 AVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYL 495

Query: 502 GRPWKEYSRTIIMQSYISDAIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPG 561
           GRPWK YSR + M S + D I P GWLEWN  FAL++L+Y E+MN G G+G+ +RV WPG
Sbjct: 496 GRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPG 555

Query: 562 YHRLNQTSEATNFTVAQFIEGNLWLPSTGVKYTAGL 574
           YH +  T EA+ FTVAQFI G+ WLPSTGV + +GL
Sbjct: 556 YHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

The following BLAST results are available for this feature:
BLAST of CSPI03G00050 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q430621.2e-21068.11Pectinesterase/pectinesterase inhibitor PPE8B OS=Prunus persica OX=3760 PE=2 SV=... [more]
Q9LXK71.3e-19364.66Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY71.1e-18962.19Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3... [more]
Q965751.9e-12547.24Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1[more]
P142808.2e-12444.34Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5[more]
BLAST of CSPI03G00050 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L4S00.0e+0099.83Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G000060 PE=3 SV=1[more]
A0A1S3BKA06.6e-30291.70Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490630 PE=3 SV=1[more]
A0A5D3CHH92.5e-27797.95Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00030... [more]
A0A6J1I8632.9e-25779.12Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470062 PE=3 SV=1[more]
A0A6J1GCJ96.7e-25486.35Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452924 PE=3 SV=1[more]
BLAST of CSPI03G00050 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004146188.20.0e+0099.83pectinesterase/pectinesterase inhibitor PPE8B [Cucumis sativus] >XP_031738323.1 ... [more]
XP_008448427.11.4e-30191.70PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo][more]
KAA0052899.15.2e-27797.95pectinesterase/pectinesterase inhibitor PPE8B-like [Cucumis melo var. makuwa] >T... [more]
XP_038905308.17.3e-26389.78pectinesterase/pectinesterase inhibitor PPE8B-like [Benincasa hispida][more]
KAG6596294.11.4e-25879.93Histone acetyltransferase HAC1, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of CSPI03G00050 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G43270.19.1e-19564.66Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33220.18.0e-19162.19pectin methylesterase 44 [more]
AT3G05620.12.1e-12248.89Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G49220.14.3e-12044.61Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.19.6e-12049.78pectin methylesterase PCR fragment F [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 96..235
e-value: 9.3E-12
score: 55.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 142..227
e-value: 4.4E-14
score: 53.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 262..559
e-value: 3.1E-149
score: 495.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 102..237
e-value: 1.2E-14
score: 56.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 117..235
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 256..573
e-value: 3.2E-123
score: 413.2
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 239..573
NoneNo IPR availablePANTHERPTHR31707:SF278PECTINESTERASE/PECTINESTERASE INHIBITOR 44-RELATEDcoord: 239..573
NoneNo IPR availablePANTHERPTHR31707:SF278PECTINESTERASE/PECTINESTERASE INHIBITOR 44-RELATEDcoord: 106..234
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 106..234
NoneNo IPR availableCDDcd15799PMEI-like_4coord: 94..236
e-value: 2.56744E-40
score: 141.779
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 260..572

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G00050.1CSPI03G00050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity