CSPI02G27340 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G27340
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMajor facilitator superfamily protein
LocationChr2: 23349283 .. 23353167 (-)
RNA-Seq ExpressionCSPI02G27340
SyntenyCSPI02G27340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAATTGATTAAAAAAAGAATTAGTAAGAAGTTAAGAACCACCACGTGGACAAACAACAGCATCATCACCTTCCTAAGAAGCAAAGCATGAAATGACACTCAAAATCGCCACCACCACCACCACCGCCACCACCGCCACCATCTCCGCCGTCCCACATAGTTTCCATCTTTGAAAGTTCTGCCTGTATCTTCTTCTATTTTCGCATCCAATCGTTTTTTTCCTACTCTCTACGATGTCCAAATCCAATTCCGATAATCCGATCCGGAAGGTTCTCGGATTGGGATATTGGGTTCAGGGTTTCCGTTGTTTTCCATGGATGGCGGTTAACTTTTTCCTCAAGGACGGCCTCAATGTCGACCCTTCTACTCTTCAGCTTCTTCAAAATTCCGCCAATCTTCCCATGGTTGCCAAGCCTCTCTACGGGATTCTCTCTGATGCCGTTTATATTTCCGGCCAACATCGCATCCCTTACATCGCCATTGGAGGTGAGTTCTTCTGAATTGCTTTAATCTCTGATTCGGTTAATGGAAGCGTTTTTTTTTTTTTTTTTAACACCGATTGTGGCCTTTTGTTCTGTGCCTGAATTCGATTGTGTTCGTTATTACGTTTGTTTGTTGTTGTGGAAAATCTGGAATATGTTTATTAAACAAGTTGAATTCTCTTGTTTCTTCTTTTGAAATCAATTTCGGATCACTCTTTGAATTGTTTAGAATTTTGATGAAACTACATTGTAGTGATGGCGTACAAGTCCTGAGGCCACTCCTCAATTACTTAGGCTTCTTTCATACCCATTAACCTTATGAACACTATTCTCACCTACTGATTTCTTTGTCCGATGTTTCAAAACCCATGTCAAGTTATTAAAGTTAGAAGTGAAAGTAGTTTTCGTTTTGAGATATTAGTTTAGAATCCAAATCTTTACTTGTAACAGATGTAGACCATGATTATGAAATGGTGAGAAAAGAATAATAATTTTCAAACAGAAAAAGAAAAACTAAGCCCTCATTGACTATTTGGTCTTTGGTTTTCAGTTTTTGAAAATGAACTTTATAAACAACCCTTTCATTTCTAATTCTCACCATGATTATGAAATGGTGAGAAAAGAATAATAATTTTCAAACAGAAAAAGAAAAACTAAGCCCTCATTGACTATTTGGTCTTGGGTTTTCAGTTTTTGAAAATGAACTTTATAAACACCTCTTTCATTTCTAAGTTCCTTGTTTTGTTATCTTATTTTTACCATTTTTTTAAAAACAAAACCAAGTTTTGAAAACTAAAAAAAGAGATTTTAAAAAAGTATTTGTTTTCAAAATTTGGCTAAGAATTCAAACTCTTTTACTTGATAAATATCCAAACCAGAAATGCACGAAAATATGTTTAATGTGAGTGGGGGAGTCTTGTAAAAAATGTTCAGTGTGGGAGTGCTTGTAAATAAATTACATCTAGCACAAGAGTAAAATATTTGATGACGTATTAACAAAGTTCGTCAACCATTTAGGCCAAAAGCACTTTGCATTTGTTTTCAAGTACCTTAAGGTAGTTATGACGTTTTTCAAAGCGATTTATGCTAATATCAAACATGATGATTTTTGTGAGGGAGATGCCTTCTGGCATAGTGTAAAATGATTTTCACCTTGCCAAAGCACCTTCACGTTATTGATTTATAAAACAATACTTTTACATAAATGCTTCCCTTTTTGGGTAAGCAAGGTTGGCAACAGAGAGGTAGACAGAAGGCAGCCTTCTTCCAGTATCTTTAAAAGTAGCTTATTAGAACTGTCAACTGAATTGCTTTTTTTGCATTGCCTGTTCATCAGTTTCTCTATAAACTGTTTTACATGATTGCTTTTGTTAACCATAGGCTTCCTGCGAACAGGCATTATATAAATCTGCACTTCACTGCTGATTGTATGAGTTTTTTGGAGAAATGAATTGGGATTGCCACTGATATGTATTGAATAGTAATTAAAAGATGTCAGAACTGCTTTTAAGGCATTAAACTATATGCTGGAGCCTTAGGAAGCTTGGCACCAAACATTTATCGTGAGTTATAAATGTGAATTGTTCAAGTTGCTTATCAATATGTTTGATTTTTTAGCTTCTGTTTGATAACCATTTGAATTTTTGGATTTTGAAAGTTCTTTTGTTACATTACAAAATATGTATCCTAGTTTTCATATTTCTTTTAGTAAACATTTTGATTCTTGACCAGATTTCCATAACAAAAACAACCTTTAAGAATTTCAAAACTCAGACTTTGAACTTTGAAAACGCCCTTATTTAAGAAAGTAGAAAATATAACAAAGAAAAACCAATGAGTGGAAGTAGTGGTTACAAGCTTAATTTTCAATGACTAAAAAATAAAAAATAAATGGTTATCAAATGAAGCTTTTAATTTGTATGATATTTACTTGAAACTCTCTAATTGTTTGGTCAATTTTGAAAAATTATTGATTCCTCTTCTGGTTTATGTTGATGCAGCTTTCTTACAGGCAGTGTCATGGTTACCCATAGCACTTATGCCACCATCTAGGATTTCAGTTTTCACCATCAGCATATATCTTCTACTTAGCAACCTCGGTGCTTCAATAGCTGAGGTTGCTAACGATGCCATCGTTGCAGAGGCAGGAAAACAGCCCACTTCTTCCAAGAATTCTCGAGCATCTTCCTCGGGTGAGCTCCAGGCTTTTGTTTGGGTTGCATCTTCTGCTGGTGGGATTCTTGGAAATCTTCTTGGAGGCGTTGCTATTGATAGGTTCTCACCCCAAGCAATGTTCCTATTCTTTGGCCTTCTCCTTTCACTTCAATTCTTCTTAACCATCATTGTTCGTGAACGCTCTCTTAATCTCCCTCAAAGCTCAATGAATGCTGGTATCAACAAACAACTCTCAGAACTCGCAGCCGTGCTAAAAAGACCTGAAATTGCTTATTCCATAATATGGCTTGCTGTATCTTACAGCATAACACCTGCATTGACTGGCACCATGTTTTTTTATCAGACAGAGCATTTGAAAATTGAGTCGTCTGTTTTAGGCATCTCTAAAGTTTTTGGCCAAGCAGCCCTACTGTTATGGGGCGTAATTTACAATCGGCATCTGAAGTCAATCCCGTCTAGGAAACTTATTTCAAGTATTCAGGTTTTAATGGCGGTGTTTATGATCTCAGACATGCTGTTCGTGAAGGGATTCTATCGAGAAATGGGCGTGCCAGACTTCATATATGTTGTTTTGTTCTCTGGACTTTCAGAGGTTCTGTTATTCTTCAAGATCTTGCCATTCAGTGTTTTGATTGCTCTGCTTTGCCCACCAGGATGTGAGGGCTCTCTCATGGCTTTTGTCATGTCAGCCATTGCCCTCGCGTTCATCGTGAGTGGATGTCTCGGTGTAGCGCTTGCATCTTATGTTGGTGTAACCGCAAATGACTTTTCCGGCCTTGCTCGTGGTTTAATAATACAAGCAGTGTGCACTCTTCTGCCACTGTGTTGTTCATGGTGCATTCCTGATGATATCAAAGCCAAAGACAGAAGAAAAGACTCCAAAAAACAGCTGTAACACAGTGGAAGACTTGATAGCATGGTCAGTGAATTCTATACAGATATATACACTCCATTGCATATTTATTTTCCGTTTGATAACCATTTTTTCTTCCTTTTTTTTTGCTTTAAATTTTTTTATTTTGTAGATTAAGCGCATTAAGCTTTATTCAAAACAAAGAGAGTTATCATACGGGTGCTAGTATTCAAAATTTTAAAGTTACTTTGATCATCCTACCATTTAGCTTTTTGAGATGAGTCTAGGAATGTATAGTAATTTATCACGAACATCTCAGGTGGTTTTATATGGTGATGTAATATTTCGATATCTAAATAAACTGTCCTTTTA

mRNA sequence

AAAAAAATTGATTAAAAAAAGAATTAGTAAGAAGTTAAGAACCACCACGTGGACAAACAACAGCATCATCACCTTCCTAAGAAGCAAAGCATGAAATGACACTCAAAATCGCCACCACCACCACCACCGCCACCACCGCCACCATCTCCGCCGTCCCACATAGTTTCCATCTTTGAAAGTTCTGCCTGTATCTTCTTCTATTTTCGCATCCAATCGTTTTTTTCCTACTCTCTACGATGTCCAAATCCAATTCCGATAATCCGATCCGGAAGGTTCTCGGATTGGGATATTGGGTTCAGGGTTTCCGTTGTTTTCCATGGATGGCGGTTAACTTTTTCCTCAAGGACGGCCTCAATGTCGACCCTTCTACTCTTCAGCTTCTTCAAAATTCCGCCAATCTTCCCATGGTTGCCAAGCCTCTCTACGGGATTCTCTCTGATGCCGTTTATATTTCCGGCCAACATCGCATCCCTTACATCGCCATTGGAGCTTTCTTACAGGCAGTGTCATGGTTACCCATAGCACTTATGCCACCATCTAGGATTTCAGTTTTCACCATCAGCATATATCTTCTACTTAGCAACCTCGGTGCTTCAATAGCTGAGGTTGCTAACGATGCCATCGTTGCAGAGGCAGGAAAACAGCCCACTTCTTCCAAGAATTCTCGAGCATCTTCCTCGGGTGAGCTCCAGGCTTTTGTTTGGGTTGCATCTTCTGCTGGTGGGATTCTTGGAAATCTTCTTGGAGGCGTTGCTATTGATAGGTTCTCACCCCAAGCAATGTTCCTATTCTTTGGCCTTCTCCTTTCACTTCAATTCTTCTTAACCATCATTGTTCGTGAACGCTCTCTTAATCTCCCTCAAAGCTCAATGAATGCTGGTATCAACAAACAACTCTCAGAACTCGCAGCCGTGCTAAAAAGACCTGAAATTGCTTATTCCATAATATGGCTTGCTGTATCTTACAGCATAACACCTGCATTGACTGGCACCATGTTTTTTTATCAGACAGAGCATTTGAAAATTGAGTCGTCTGTTTTAGGCATCTCTAAAGTTTTTGGCCAAGCAGCCCTACTGTTATGGGGCGTAATTTACAATCGGCATCTGAAGTCAATCCCGTCTAGGAAACTTATTTCAAGTATTCAGGTTTTAATGGCGGTGTTTATGATCTCAGACATGCTGTTCGTGAAGGGATTCTATCGAGAAATGGGCGTGCCAGACTTCATATATGTTGTTTTGTTCTCTGGACTTTCAGAGGTTCTGTTATTCTTCAAGATCTTGCCATTCAGTGTTTTGATTGCTCTGCTTTGCCCACCAGGATGTGAGGGCTCTCTCATGGCTTTTGTCATGTCAGCCATTGCCCTCGCGTTCATCGTGAGTGGATGTCTCGGTGTAGCGCTTGCATCTTATGTTGGTGTAACCGCAAATGACTTTTCCGGCCTTGCTCGTGGTTTAATAATACAAGCAGTGTGCACTCTTCTGCCACTGTGTTGTTCATGGTGCATTCCTGATGATATCAAAGCCAAAGACAGAAGAAAAGACTCCAAAAAACAGCTGTAACACAGTGGAAGACTTGATAGCATGGTCAGTGAATTCTATACAGATATATACACTCCATTGCATATTTATTTTCCGTTTGATAACCATTTTTTCTTCCTTTTTTTTTGCTTTAAATTTTTTTATTTTGTAGATTAAGCGCATTAAGCTTTATTCAAAACAAAGAGAGTTATCATACGGGTGCTAGTATTCAAAATTTTAAAGTTACTTTGATCATCCTACCATTTAGCTTTTTGAGATGAGTCTAGGAATGTATAGTAATTTATCACGAACATCTCAGGTGGTTTTATATGGTGATGTAATATTTCGATATCTAAATAAACTGTCCTTTTA

Coding sequence (CDS)

ATGTCCAAATCCAATTCCGATAATCCGATCCGGAAGGTTCTCGGATTGGGATATTGGGTTCAGGGTTTCCGTTGTTTTCCATGGATGGCGGTTAACTTTTTCCTCAAGGACGGCCTCAATGTCGACCCTTCTACTCTTCAGCTTCTTCAAAATTCCGCCAATCTTCCCATGGTTGCCAAGCCTCTCTACGGGATTCTCTCTGATGCCGTTTATATTTCCGGCCAACATCGCATCCCTTACATCGCCATTGGAGCTTTCTTACAGGCAGTGTCATGGTTACCCATAGCACTTATGCCACCATCTAGGATTTCAGTTTTCACCATCAGCATATATCTTCTACTTAGCAACCTCGGTGCTTCAATAGCTGAGGTTGCTAACGATGCCATCGTTGCAGAGGCAGGAAAACAGCCCACTTCTTCCAAGAATTCTCGAGCATCTTCCTCGGGTGAGCTCCAGGCTTTTGTTTGGGTTGCATCTTCTGCTGGTGGGATTCTTGGAAATCTTCTTGGAGGCGTTGCTATTGATAGGTTCTCACCCCAAGCAATGTTCCTATTCTTTGGCCTTCTCCTTTCACTTCAATTCTTCTTAACCATCATTGTTCGTGAACGCTCTCTTAATCTCCCTCAAAGCTCAATGAATGCTGGTATCAACAAACAACTCTCAGAACTCGCAGCCGTGCTAAAAAGACCTGAAATTGCTTATTCCATAATATGGCTTGCTGTATCTTACAGCATAACACCTGCATTGACTGGCACCATGTTTTTTTATCAGACAGAGCATTTGAAAATTGAGTCGTCTGTTTTAGGCATCTCTAAAGTTTTTGGCCAAGCAGCCCTACTGTTATGGGGCGTAATTTACAATCGGCATCTGAAGTCAATCCCGTCTAGGAAACTTATTTCAAGTATTCAGGTTTTAATGGCGGTGTTTATGATCTCAGACATGCTGTTCGTGAAGGGATTCTATCGAGAAATGGGCGTGCCAGACTTCATATATGTTGTTTTGTTCTCTGGACTTTCAGAGGTTCTGTTATTCTTCAAGATCTTGCCATTCAGTGTTTTGATTGCTCTGCTTTGCCCACCAGGATGTGAGGGCTCTCTCATGGCTTTTGTCATGTCAGCCATTGCCCTCGCGTTCATCGTGAGTGGATGTCTCGGTGTAGCGCTTGCATCTTATGTTGGTGTAACCGCAAATGACTTTTCCGGCCTTGCTCGTGGTTTAATAATACAAGCAGTGTGCACTCTTCTGCCACTGTGTTGTTCATGGTGCATTCCTGATGATATCAAAGCCAAAGACAGAAGAAAAGACTCCAAAAAACAGCTGTAA

Protein sequence

MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLAVSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLISSIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCSWCIPDDIKAKDRRKDSKKQL*
Homology
BLAST of CSPI02G27340 vs. ExPASy Swiss-Prot
Match: Q9XIQ7 (Probable folate-biopterin transporter 7 OS=Arabidopsis thaliana OX=3702 GN=At1g64890 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.3e-148
Identity = 275/428 (64.25%), Postives = 342/428 (79.91%), Query Frame = 0

Query: 8   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 67
           NPIR +LG G++VQGFR FPW+  NFFL + L V+PS LQLLQNSANLPMVAKP+YG++S
Sbjct: 17  NPIRWLLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVS 76

Query: 68  DAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVAND 127
           D+VY  GQHRIPYIA+GA LQA+SWL IA +  S +S+  +SIYLLLSNLGAS+ EVAND
Sbjct: 77  DSVYFFGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVAND 136

Query: 128 AIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFG 187
           AIVAE+GKQ TS      + SGEL +FVW+ SS GGILGNLLGG+AI  FS Q+ FL FG
Sbjct: 137 AIVAESGKQKTSE-----TQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFG 196

Query: 188 LLLSLQFFLTIIVRERSLNLPQS-SMNAGINKQLSELAAVLKRPEIAYSIIWLAVSYSIT 247
           +L  LQF +TI +RE+SLNLP++ S   GI K LS+L+ VL++PEI+YSI W+AVS ++ 
Sbjct: 197 ILALLQFLVTINIREKSLNLPENPSPAGGIRKHLSDLSHVLRKPEISYSIAWIAVSTAVV 256

Query: 248 PALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLISSIQVLM 307
           P LTGTMFFYQT+ LKI++S+LGISKVFGQ A+LLWG  YNR LK++  RKL+++IQV +
Sbjct: 257 PVLTGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQVTI 316

Query: 308 AVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPPGCEGSL 367
           A F+ISD+LFVKG YR++GV D +YV+ FSG  E L +FKILPF+VL+A LCPPGCEGSL
Sbjct: 317 AFFVISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEGSL 376

Query: 368 MAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCSWCIPDD 427
           MAFVMSAIALAFIVSG LG+ LAS+VGVT +DFSG  RGL I+A C  +PL  +  I D+
Sbjct: 377 MAFVMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIYDE 436

Query: 428 IKAKDRRK 435
            + K++ K
Sbjct: 437 AETKEKSK 439

BLAST of CSPI02G27340 vs. ExPASy Swiss-Prot
Match: F4I5Q2 (Probable folate-biopterin transporter 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g04570 PE=2 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 7.5e-93
Identity = 191/419 (45.58%), Postives = 278/419 (66.35%), Query Frame = 0

Query: 10  IRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDA 69
           I  + GLGYWVQG RCFPW+A+NF +   L + PSTLQL+Q S +LPMVAKPLYG+LSD 
Sbjct: 87  ISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSCSLPMVAKPLYGVLSDV 146

Query: 70  VYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVANDAI 129
           +YI    R+PYIAIG FLQ ++W  + +   +R  + ++   +LLSNLGASI EVA DA+
Sbjct: 147 LYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVLLSNLGASITEVAKDAL 206

Query: 130 VAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFGLL 189
           VAE G         R   +G LQ++  +AS+AGG+LGNLLGG  +    P+  FL F  L
Sbjct: 207 VAEYG--------LRYRING-LQSYALMASAAGGVLGNLLGGYLLLTTPPKISFLVFSAL 266

Query: 190 LSLQFFLTIIVRERSLNLPQ----SSMNAGINKQLSELAAVLKRPEIAYSIIWLAVSYSI 249
           LSLQ  +++  +E S  LP+    SS+   + KQ+S L   ++  EI+  +IW  VS ++
Sbjct: 267 LSLQLVVSLSSKEESFGLPRIAETSSVLESVKKQISNLKEAIQADEISQPLIWAVVSIAM 326

Query: 250 TPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLISSIQVL 309
            P L+G++F YQT+ L ++ SV+G+SKV GQ  LL   V+Y+R+LK++P R LI  IQ+L
Sbjct: 327 VPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLPMRPLIHIIQLL 386

Query: 310 MAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPPGCEGS 369
             + ++ D + VK      G+ + +YV+ FS L+E+L  FKILPF+V +A +CP GCEGS
Sbjct: 387 YGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRLASMCPQGCEGS 446

Query: 370 LMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCSWCIP 425
           + +F+ S + L+ IVS  LGV LA+ +G+T++++S L+ G++IQ++  L PLC    +P
Sbjct: 447 VTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAALAPLCFMHLVP 496

BLAST of CSPI02G27340 vs. ExPASy Swiss-Prot
Match: O22780 (Probable folate-biopterin transporter 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g33280 PE=3 SV=2)

HSP 1 Score: 335.5 bits (859), Expect = 9.1e-91
Identity = 187/434 (43.09%), Postives = 285/434 (65.67%), Query Frame = 0

Query: 16  LGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYISGQ 75
           LGYWVQG RCF W+A+NF +   LN+ PSTLQL+Q +A+LPMVAKPLYG+LSD +YI G 
Sbjct: 90  LGYWVQGLRCFSWLALNFHMAHCLNLKPSTLQLVQYTASLPMVAKPLYGVLSDVLYIGGA 149

Query: 76  HRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVANDAIVAEAGK 135
            R+PYI++G  LQ ++W  +A+ P +R  + ++  ++LLSNLGASI EV+ DA+VAE G 
Sbjct: 150 RRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSNLGASITEVSQDALVAEYG- 209

Query: 136 QPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFGLLLSLQFF 195
                   R   +G LQ++  +AS+ GGILGNLLGG  + +  P+ +FL F  LLSLQ  
Sbjct: 210 -------LRYQING-LQSYALMASAVGGILGNLLGGYCLLKTPPRILFLAFTALLSLQLI 269

Query: 196 LTIIVRERSLNLPQ--------SSMNAGINKQLSELAAVLKRPEIAYSIIWLAVSYSITP 255
           +++  +E S+NLP+        SS+   + KQ  +L  +++  EI+  +IW+  S ++ P
Sbjct: 270 VSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQVDEISQPLIWIVSSIALVP 329

Query: 256 ALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLISSIQVLMA 315
            L+G++F YQT+ L ++ SV+G+SKV GQ  LL   V+Y+R+ K +P R LI  +Q+L A
Sbjct: 330 LLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYWKKLPMRALIHIVQLLYA 389

Query: 316 VFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPPGCEGSLM 375
             ++ D + VK      G+ +  +V+ FS ++E+L  FKILPFSVL+A +CP GCEGS+ 
Sbjct: 390 FSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKILPFSVLLANMCPGGCEGSIT 449

Query: 376 AFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCSWCIP--- 435
           +F+ S + L+ +VSG  GV +A+ +G+T+ ++S L  G++IQ++  L+PL     +P   
Sbjct: 450 SFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILIQSLAALVPLWFIHYVPMLE 509

Query: 436 DDIKAKDRRKDSKK 439
              + + +R  SKK
Sbjct: 510 PGFEREGKRAMSKK 514

BLAST of CSPI02G27340 vs. ExPASy Swiss-Prot
Match: Q8RWQ5 (Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g54860 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 4.7e-55
Identity = 151/467 (32.33%), Postives = 230/467 (49.25%), Query Frame = 0

Query: 18  YWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYISGQHR 77
           Y+ QGFR F W AV++ LKD L + PS  Q + + A  P   KPLYGI+SD + I G+ R
Sbjct: 24  YFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCIPIGGKKR 83

Query: 78  IPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVANDAIVAEAGKQP 137
            PY+ I   L  V WL + L   SR S   + I+L + NLG+++A+V  DA++AEA    
Sbjct: 84  TPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMADVVIDAMIAEA---- 143

Query: 138 TSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFGLLLSLQFFLT 197
              +  +AS +G+LQ+  W A + GG+ G+LLGG A+     + +FL F +L +LQ    
Sbjct: 144 --VRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTVLPALQLLSC 203

Query: 198 IIVRERSLN---LP---------QSSMNAGIN---------------------------- 257
            +V E   N   LP         + S+ +  N                            
Sbjct: 204 ALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGKKGDSNGKSE 263

Query: 258 ---KQLSELAA---------------VLKRPEIAYSIIWLAVSYSITPALTGTMFFYQTE 317
              KQ   LA+                 K+P I   + W  +++   P L+  MF+YQTE
Sbjct: 264 TQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPNLSTVMFYYQTE 323

Query: 318 HLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLISSIQVLMAVFMISDMLFVKG 377
            L+++++ LG ++V G   L+    IYNR+L+ +  RK +    + ++V ++ DM+ V  
Sbjct: 324 VLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSVTILLDMVLVSR 383

Query: 378 FYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPPGCEGSLMAFVMSAIALAFI 427
                GV D   V+  S L + +   K +PF +L   LCPPG EG+L A  MS   L   
Sbjct: 384 ANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFALFMSINNLGNT 443

BLAST of CSPI02G27340 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 160.6 bits (405), Expect = 4.0e-38
Identity = 118/430 (27.44%), Postives = 210/430 (48.84%), Query Frame = 0

Query: 8   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 67
           +P    + + Y+VQG      +AV+F+LKD L++DP+   ++   ++LP + KPLYG +S
Sbjct: 124 SPDNVAVAMVYFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFIS 183

Query: 68  DAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVAND 127
           D+V + G  R  Y+ +   L A SW   +LM     S ++ +  +LL +L  + ++V  D
Sbjct: 184 DSVPLFGYRRRSYLVLSGLLGAFSW---SLMAGFVDSKYSAAFCILLGSLSVAFSDVVVD 243

Query: 128 AIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFG 187
           ++V E       ++    S SG LQ+  W +S+ GGI+ +   G  ++ +  + +F    
Sbjct: 244 SMVVE------RARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTA 303

Query: 188 LLLSLQFFLTIIVRERSLNLPQSSMNAGI-----------NKQLSELAAVLKRPEIAYSI 247
           LL  +   + ++V E+ +  P S     I            + + +L   +K+P +    
Sbjct: 304 LLPLITSAVAVLVNEQRVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPT 363

Query: 248 IWLAVSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSR 307
           +++ + +  TP     MF++ T  L      LG  K+    A LL   +YN  LK++P R
Sbjct: 364 LFIFL-WQATPHSDSAMFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLR 423

Query: 308 KLISSIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIAL 367
           K+     +      ++ ++ V GF R++G+ D  + +  S +  VL     +P  VL A 
Sbjct: 424 KIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAAR 483

Query: 368 LCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLP 427
           LCP G E +L A +MS      ++ G +G  L    G+T + F  L+  +I+  + +LLP
Sbjct: 484 LCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLP 543

BLAST of CSPI02G27340 vs. ExPASy TrEMBL
Match: A0A0A0LTK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G431200 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 4.7e-239
Identity = 439/440 (99.77%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
           AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
           VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQVLMAVFMISDMLFVKG YREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQL 441
           WCIPDDIKAKDRRKDSKKQL
Sbjct: 421 WCIPDDIKAKDRRKDSKKQL 440

BLAST of CSPI02G27340 vs. ExPASy TrEMBL
Match: A0A5A7VD92 (Putative folate-biopterin transporter 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001470 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.8e-235
Identity = 433/440 (98.41%), Postives = 436/440 (99.09%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           M+KSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MAKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
           AMFLFFGLLLSLQFFLTIIVRERSLNLPQSS NAGINKQLSELAAVLKRPEIAYSI+WLA
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSTNAGINKQLSELAAVLKRPEIAYSIMWLA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
           VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLI+
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIA 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQV MAVFMISDMLFVKG YREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP
Sbjct: 301 SIQVSMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQL 441
           WCIPDDIKAKDRRKDSKKQL
Sbjct: 421 WCIPDDIKAKDRRKDSKKQL 440

BLAST of CSPI02G27340 vs. ExPASy TrEMBL
Match: A0A1S3CHR5 (probable folate-biopterin transporter 7 OS=Cucumis melo OX=3656 GN=LOC103501083 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.8e-235
Identity = 433/440 (98.41%), Postives = 436/440 (99.09%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           M+KSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MAKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
           AMFLFFGLLLSLQFFLTIIVRERSLNLPQSS NAGINKQLSELAAVLKRPEIAYSI+WLA
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSTNAGINKQLSELAAVLKRPEIAYSIMWLA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
           VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLI+
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIA 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQV MAVFMISDMLFVKG YREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP
Sbjct: 301 SIQVSMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQL 441
           WCIPDDIKAKDRRKDSKKQL
Sbjct: 421 WCIPDDIKAKDRRKDSKKQL 440

BLAST of CSPI02G27340 vs. ExPASy TrEMBL
Match: A0A6J1FV96 (probable folate-biopterin transporter 7 OS=Cucurbita moschata OX=3662 GN=LOC111448420 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.6e-218
Identity = 402/439 (91.57%), Postives = 414/439 (94.31%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MS+SN++NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGA LQAVSW+PIA M PS ISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGG+ IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
            MFLFFGLLL LQF LTIIVRERSLNLPQSS +AGI KQLS+L  VLKRPEI YSI W A
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQVLMAVFMISDMLFVKG YREMG+PDF+YVVLFSGLSEVLLFFKILPFSVLIA LCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGL  GLIIQAVCT+LPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQ 440
           WCIPDD+KAK R KDSKKQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQ 439

BLAST of CSPI02G27340 vs. ExPASy TrEMBL
Match: A0A6J1JEB7 (probable folate-biopterin transporter 7 OS=Cucurbita maxima OX=3661 GN=LOC111484245 PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.5e-218
Identity = 402/439 (91.57%), Postives = 414/439 (94.31%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MS+SN++NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGA LQAVSW+PIA M PS ISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGG+ IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
            MFLFFGLLL LQF LTIIVRERSLNLPQSS +AGI KQLS+L  VLKRPEI YSI W A
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
            SYSI PALTGTMFFYQTE+LKIESSVLGISKV GQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTENLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQVLMAVFMISDMLFVKG YREMG+PDF+YVVLFSGLSEVLLFFKILPFSVLIA LCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGL  GLIIQAVCTLLPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQ 440
           WCIPDD+KAK R KDSKKQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQ 439

BLAST of CSPI02G27340 vs. NCBI nr
Match: XP_004151911.1 (probable folate-biopterin transporter 7 [Cucumis sativus] >XP_011650057.1 probable folate-biopterin transporter 7 [Cucumis sativus] >KGN63351.1 hypothetical protein Csa_022392 [Cucumis sativus])

HSP 1 Score: 836.6 bits (2160), Expect = 9.6e-239
Identity = 439/440 (99.77%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
           AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
           VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQVLMAVFMISDMLFVKG YREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQL 441
           WCIPDDIKAKDRRKDSKKQL
Sbjct: 421 WCIPDDIKAKDRRKDSKKQL 440

BLAST of CSPI02G27340 vs. NCBI nr
Match: XP_008462793.1 (PREDICTED: probable folate-biopterin transporter 7 [Cucumis melo] >KAA0066312.1 putative folate-biopterin transporter 7 [Cucumis melo var. makuwa] >TYK00965.1 putative folate-biopterin transporter 7 [Cucumis melo var. makuwa])

HSP 1 Score: 824.7 bits (2129), Expect = 3.8e-235
Identity = 433/440 (98.41%), Postives = 436/440 (99.09%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           M+KSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MAKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
           AMFLFFGLLLSLQFFLTIIVRERSLNLPQSS NAGINKQLSELAAVLKRPEIAYSI+WLA
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSTNAGINKQLSELAAVLKRPEIAYSIMWLA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
           VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLI+
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIA 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQV MAVFMISDMLFVKG YREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP
Sbjct: 301 SIQVSMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQL 441
           WCIPDDIKAKDRRKDSKKQL
Sbjct: 421 WCIPDDIKAKDRRKDSKKQL 440

BLAST of CSPI02G27340 vs. NCBI nr
Match: XP_038900302.1 (probable folate-biopterin transporter 7 [Benincasa hispida] >XP_038900303.1 probable folate-biopterin transporter 7 [Benincasa hispida] >XP_038900304.1 probable folate-biopterin transporter 7 [Benincasa hispida])

HSP 1 Score: 798.1 bits (2060), Expect = 3.8e-227
Identity = 416/439 (94.76%), Postives = 427/439 (97.27%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           M+ SNSDNPI+KVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MATSNSDNPIQKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSW+PIALMPPSRISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGK+PTSSKNSRASSSGELQAFVWVASSAGGILGNLLGG+AIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKRPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
           AMFL FGLLLSLQFFLTIIVRE SLNLP+SS NAGINKQLSELAAVLKRP+IAYSI+W A
Sbjct: 181 AMFLSFGLLLSLQFFLTIIVRENSLNLPRSSTNAGINKQLSELAAVLKRPDIAYSILWFA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
            SYSI PALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKS+PSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSVPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQV MAVFMISD+LFVKG YREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIA LCPP
Sbjct: 301 SIQVSMAVFMISDVLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVG+TANDFSGLA GLIIQAVCTLLPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGITANDFSGLAHGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQ 440
           WCIPDDIKAKDRRKDSKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of CSPI02G27340 vs. NCBI nr
Match: KAG6571212.1 (putative folate-biopterin transporter 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 769.6 bits (1986), Expect = 1.4e-218
Identity = 403/439 (91.80%), Postives = 414/439 (94.31%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MS+SN++NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGA LQAVSW+PIA M PS ISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGG+ IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
            MFLFFGLLL LQF LTIIVRERSLNLPQSS +AGI KQLS+L  VLKRPEI YSI W A
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQVLMAVFMISDMLFVKG YREMGVPDF+YVVLFSGLSEVLLFFKILPFSVLIA LCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGL  GLIIQAVCT+LPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQ 440
           WCIPDD+KAK R KDSKKQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQ 439

BLAST of CSPI02G27340 vs. NCBI nr
Match: XP_022943764.1 (probable folate-biopterin transporter 7 [Cucurbita moschata] >XP_022943765.1 probable folate-biopterin transporter 7 [Cucurbita moschata])

HSP 1 Score: 768.5 bits (1983), Expect = 3.2e-218
Identity = 402/439 (91.57%), Postives = 414/439 (94.31%), Query Frame = 0

Query: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MS+SN++NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYISGQHRIPYIAIGA LQAVSW+PIA M PS ISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGG+ IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240
            MFLFFGLLL LQF LTIIVRERSLNLPQSS +AGI KQLS+L  VLKRPEI YSI W A
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360
           SIQVLMAVFMISDMLFVKG YREMG+PDF+YVVLFSGLSEVLLFFKILPFSVLIA LCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGL  GLIIQAVCT+LPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 421 WCIPDDIKAKDRRKDSKKQ 440
           WCIPDD+KAK R KDSKKQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQ 439

BLAST of CSPI02G27340 vs. TAIR 10
Match: AT1G64890.1 (Major facilitator superfamily protein )

HSP 1 Score: 527.7 bits (1358), Expect = 8.9e-150
Identity = 275/428 (64.25%), Postives = 342/428 (79.91%), Query Frame = 0

Query: 8   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 67
           NPIR +LG G++VQGFR FPW+  NFFL + L V+PS LQLLQNSANLPMVAKP+YG++S
Sbjct: 17  NPIRWLLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVS 76

Query: 68  DAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVAND 127
           D+VY  GQHRIPYIA+GA LQA+SWL IA +  S +S+  +SIYLLLSNLGAS+ EVAND
Sbjct: 77  DSVYFFGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVAND 136

Query: 128 AIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFG 187
           AIVAE+GKQ TS      + SGEL +FVW+ SS GGILGNLLGG+AI  FS Q+ FL FG
Sbjct: 137 AIVAESGKQKTSE-----TQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFG 196

Query: 188 LLLSLQFFLTIIVRERSLNLPQS-SMNAGINKQLSELAAVLKRPEIAYSIIWLAVSYSIT 247
           +L  LQF +TI +RE+SLNLP++ S   GI K LS+L+ VL++PEI+YSI W+AVS ++ 
Sbjct: 197 ILALLQFLVTINIREKSLNLPENPSPAGGIRKHLSDLSHVLRKPEISYSIAWIAVSTAVV 256

Query: 248 PALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLISSIQVLM 307
           P LTGTMFFYQT+ LKI++S+LGISKVFGQ A+LLWG  YNR LK++  RKL+++IQV +
Sbjct: 257 PVLTGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQVTI 316

Query: 308 AVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPPGCEGSL 367
           A F+ISD+LFVKG YR++GV D +YV+ FSG  E L +FKILPF+VL+A LCPPGCEGSL
Sbjct: 317 AFFVISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEGSL 376

Query: 368 MAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCSWCIPDD 427
           MAFVMSAIALAFIVSG LG+ LAS+VGVT +DFSG  RGL I+A C  +PL  +  I D+
Sbjct: 377 MAFVMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIYDE 436

Query: 428 IKAKDRRK 435
            + K++ K
Sbjct: 437 AETKEKSK 439

BLAST of CSPI02G27340 vs. TAIR 10
Match: AT1G04570.1 (Major facilitator superfamily protein )

HSP 1 Score: 342.4 bits (877), Expect = 5.3e-94
Identity = 191/419 (45.58%), Postives = 278/419 (66.35%), Query Frame = 0

Query: 10  IRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDA 69
           I  + GLGYWVQG RCFPW+A+NF +   L + PSTLQL+Q S +LPMVAKPLYG+LSD 
Sbjct: 87  ISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSCSLPMVAKPLYGVLSDV 146

Query: 70  VYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVANDAI 129
           +YI    R+PYIAIG FLQ ++W  + +   +R  + ++   +LLSNLGASI EVA DA+
Sbjct: 147 LYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVLLSNLGASITEVAKDAL 206

Query: 130 VAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFGLL 189
           VAE G         R   +G LQ++  +AS+AGG+LGNLLGG  +    P+  FL F  L
Sbjct: 207 VAEYG--------LRYRING-LQSYALMASAAGGVLGNLLGGYLLLTTPPKISFLVFSAL 266

Query: 190 LSLQFFLTIIVRERSLNLPQ----SSMNAGINKQLSELAAVLKRPEIAYSIIWLAVSYSI 249
           LSLQ  +++  +E S  LP+    SS+   + KQ+S L   ++  EI+  +IW  VS ++
Sbjct: 267 LSLQLVVSLSSKEESFGLPRIAETSSVLESVKKQISNLKEAIQADEISQPLIWAVVSIAM 326

Query: 250 TPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLISSIQVL 309
            P L+G++F YQT+ L ++ SV+G+SKV GQ  LL   V+Y+R+LK++P R LI  IQ+L
Sbjct: 327 VPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLPMRPLIHIIQLL 386

Query: 310 MAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPPGCEGS 369
             + ++ D + VK      G+ + +YV+ FS L+E+L  FKILPF+V +A +CP GCEGS
Sbjct: 387 YGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRLASMCPQGCEGS 446

Query: 370 LMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCSWCIP 425
           + +F+ S + L+ IVS  LGV LA+ +G+T++++S L+ G++IQ++  L PLC    +P
Sbjct: 447 VTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAALAPLCFMHLVP 496

BLAST of CSPI02G27340 vs. TAIR 10
Match: AT2G33280.1 (Major facilitator superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.7e-74
Identity = 161/393 (40.97%), Postives = 252/393 (64.12%), Query Frame = 0

Query: 57  MVAKPLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSN 116
           MVAKPLYG+LSD +YI G  R+PYI++G  LQ ++W  +A+ P +R  + ++  ++LLSN
Sbjct: 1   MVAKPLYGVLSDVLYIGGARRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSN 60

Query: 117 LGASIAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDR 176
           LGASI EV+ DA+VAE G         R   +G LQ++  +AS+ GGILGNLLGG  + +
Sbjct: 61  LGASITEVSQDALVAEYG--------LRYQING-LQSYALMASAVGGILGNLLGGYCLLK 120

Query: 177 FSPQAMFLFFGLLLSLQFFLTIIVRERSLNLPQ--------SSMNAGINKQLSELAAVLK 236
             P+ +FL F  LLSLQ  +++  +E S+NLP+        SS+   + KQ  +L  +++
Sbjct: 121 TPPRILFLAFTALLSLQLIVSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQ 180

Query: 237 RPEIAYSIIWLAVSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNR 296
             EI+  +IW+  S ++ P L+G++F YQT+ L ++ SV+G+SKV GQ  LL   V+Y+R
Sbjct: 181 VDEISQPLIWIVSSIALVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDR 240

Query: 297 HLKSIPSRKLISSIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKIL 356
           + K +P R LI  +Q+L A  ++ D + VK      G+ +  +V+ FS ++E+L  FKIL
Sbjct: 241 YWKKLPMRALIHIVQLLYAFSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKIL 300

Query: 357 PFSVLIALLCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLII 416
           PFSVL+A +CP GCEGS+ +F+ S + L+ +VSG  GV +A+ +G+T+ ++S L  G++I
Sbjct: 301 PFSVLLANMCPGGCEGSITSFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILI 360

Query: 417 QAVCTLLPLCCSWCIP---DDIKAKDRRKDSKK 439
           Q++  L+PL     +P      + + +R  SKK
Sbjct: 361 QSLAALVPLWFIHYVPMLEPGFEREGKRAMSKK 384

BLAST of CSPI02G27340 vs. TAIR 10
Match: AT5G54860.1 (Major facilitator superfamily protein )

HSP 1 Score: 216.9 bits (551), Expect = 3.4e-56
Identity = 151/467 (32.33%), Postives = 230/467 (49.25%), Query Frame = 0

Query: 18  YWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYISGQHR 77
           Y+ QGFR F W AV++ LKD L + PS  Q + + A  P   KPLYGI+SD + I G+ R
Sbjct: 24  YFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCIPIGGKKR 83

Query: 78  IPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVANDAIVAEAGKQP 137
            PY+ I   L  V WL + L   SR S   + I+L + NLG+++A+V  DA++AEA    
Sbjct: 84  TPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMADVVIDAMIAEA---- 143

Query: 138 TSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFGLLLSLQFFLT 197
              +  +AS +G+LQ+  W A + GG+ G+LLGG A+     + +FL F +L +LQ    
Sbjct: 144 --VRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTVLPALQLLSC 203

Query: 198 IIVRERSLN---LP---------QSSMNAGIN---------------------------- 257
            +V E   N   LP         + S+ +  N                            
Sbjct: 204 ALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGKKGDSNGKSE 263

Query: 258 ---KQLSELAA---------------VLKRPEIAYSIIWLAVSYSITPALTGTMFFYQTE 317
              KQ   LA+                 K+P I   + W  +++   P L+  MF+YQTE
Sbjct: 264 TQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPNLSTVMFYYQTE 323

Query: 318 HLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLISSIQVLMAVFMISDMLFVKG 377
            L+++++ LG ++V G   L+    IYNR+L+ +  RK +    + ++V ++ DM+ V  
Sbjct: 324 VLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSVTILLDMVLVSR 383

Query: 378 FYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPPGCEGSLMAFVMSAIALAFI 427
                GV D   V+  S L + +   K +PF +L   LCPPG EG+L A  MS   L   
Sbjct: 384 ANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFALFMSINNLGNT 443

BLAST of CSPI02G27340 vs. TAIR 10
Match: AT2G32040.1 (Major facilitator superfamily protein )

HSP 1 Score: 160.6 bits (405), Expect = 2.9e-39
Identity = 118/430 (27.44%), Postives = 210/430 (48.84%), Query Frame = 0

Query: 8   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 67
           +P    + + Y+VQG      +AV+F+LKD L++DP+   ++   ++LP + KPLYG +S
Sbjct: 124 SPDNVAVAMVYFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFIS 183

Query: 68  DAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGASIAEVAND 127
           D+V + G  R  Y+ +   L A SW   +LM     S ++ +  +LL +L  + ++V  D
Sbjct: 184 DSVPLFGYRRRSYLVLSGLLGAFSW---SLMAGFVDSKYSAAFCILLGSLSVAFSDVVVD 243

Query: 128 AIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQAMFLFFG 187
           ++V E       ++    S SG LQ+  W +S+ GGI+ +   G  ++ +  + +F    
Sbjct: 244 SMVVE------RARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTA 303

Query: 188 LLLSLQFFLTIIVRERSLNLPQSSMNAGI-----------NKQLSELAAVLKRPEIAYSI 247
           LL  +   + ++V E+ +  P S     I            + + +L   +K+P +    
Sbjct: 304 LLPLITSAVAVLVNEQRVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPT 363

Query: 248 IWLAVSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSR 307
           +++ + +  TP     MF++ T  L      LG  K+    A LL   +YN  LK++P R
Sbjct: 364 LFIFL-WQATPHSDSAMFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLR 423

Query: 308 KLISSIQVLMAVFMISDMLFVKGFYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIAL 367
           K+     +      ++ ++ V GF R++G+ D  + +  S +  VL     +P  VL A 
Sbjct: 424 KIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAAR 483

Query: 368 LCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLP 427
           LCP G E +L A +MS      ++ G +G  L    G+T + F  L+  +I+  + +LLP
Sbjct: 484 LCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLP 543

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XIQ71.3e-14864.25Probable folate-biopterin transporter 7 OS=Arabidopsis thaliana OX=3702 GN=At1g6... [more]
F4I5Q27.5e-9345.58Probable folate-biopterin transporter 8, chloroplastic OS=Arabidopsis thaliana O... [more]
O227809.1e-9143.09Probable folate-biopterin transporter 9, chloroplastic OS=Arabidopsis thaliana O... [more]
Q8RWQ54.7e-5532.33Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g5... [more]
Q9SKZ54.0e-3827.44Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0LTK84.7e-23999.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G431200 PE=3 SV=1[more]
A0A5A7VD921.8e-23598.41Putative folate-biopterin transporter 7 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CHR51.8e-23598.41probable folate-biopterin transporter 7 OS=Cucumis melo OX=3656 GN=LOC103501083 ... [more]
A0A6J1FV961.6e-21891.57probable folate-biopterin transporter 7 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JEB74.5e-21891.57probable folate-biopterin transporter 7 OS=Cucurbita maxima OX=3661 GN=LOC111484... [more]
Match NameE-valueIdentityDescription
XP_004151911.19.6e-23999.77probable folate-biopterin transporter 7 [Cucumis sativus] >XP_011650057.1 probab... [more]
XP_008462793.13.8e-23598.41PREDICTED: probable folate-biopterin transporter 7 [Cucumis melo] >KAA0066312.1 ... [more]
XP_038900302.13.8e-22794.76probable folate-biopterin transporter 7 [Benincasa hispida] >XP_038900303.1 prob... [more]
KAG6571212.11.4e-21891.80putative folate-biopterin transporter 7, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022943764.13.2e-21891.57probable folate-biopterin transporter 7 [Cucurbita moschata] >XP_022943765.1 pro... [more]
Match NameE-valueIdentityDescription
AT1G64890.18.9e-15064.25Major facilitator superfamily protein [more]
AT1G04570.15.3e-9445.58Major facilitator superfamily protein [more]
AT2G33280.12.7e-7440.97Major facilitator superfamily protein [more]
AT5G54860.13.4e-5632.33Major facilitator superfamily protein [more]
AT2G32040.12.9e-3927.44Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039309Biopterin transporter familyPFAMPF03092BT1coord: 21..434
e-value: 2.4E-83
score: 280.1
IPR039309Biopterin transporter familyPANTHERPTHR31585FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTICcoord: 8..436
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 19..423
e-value: 2.3E-19
score: 71.4
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 7..433
IPR004324Folate-biopterin transporterTIGRFAMTIGR00788TIGR00788coord: 18..431
e-value: 7.5E-68
score: 227.5
NoneNo IPR availablePANTHERPTHR31585:SF2FOLATE-BIOPTERIN TRANSPORTER 7-RELATEDcoord: 8..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G27340.1CSPI02G27340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane