CSPI02G27070 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G27070
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
Location	Chr2: 23117401 .. 23120750 (+)
RNA-Seq Expression	CSPI02G27070
Synteny	CSPI02G27070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATAACATAAAACAAAATTGATGATTTAAAACTGAGTTTGGCAAGATACAAAATGCTTGGAGGGAAAGATATTTACGTGAAAAACCTAATCCATAAGGCATAAACCCCGAAAGAAATAGATTCCATTTGCACCCTAAAATCTCCTCTACCGGACCGGCGGCGGCGATGGGCTGCCTAACCATCCTCATCCTTAGGTTATGAGATCTCTTTCCAATTCTCATCTTCATCTCAAATATATATTCTCTCTCCCTAACTCTCTCATGGCTTCCTCTCTTATACAAACCCTTAAACCAGATTTAGCTCCTCAAGACCTAGACGACCTTCTCTGTTCCCCTTCACAGAAACAGCCACAACAGGAATCCTCCTCTTTTACCAATTCCACTTCCAATGACTCAACTCCTCTCCTGGGGTCGCCGACTGGATATTTGAGTCGAGAGTCGCGCATTGAACGAGCCTGGGCTCACTGGACCAAGCTCGGTCGCCCAAAGTTGATTGTAGCTCCCATGGTTGATAATTCGGAGTTGCCATTTCGTTTACTCTGCAGGAAATATGGTGCTGAGGCTGCTTATACGCCTATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGTATGTGACAATTTAAAGCCATTTTATTGTGTTAACAACTTAAATATTCTGTTTCATTTGGAATTCATGTATGTTTCCGGCTGCTTTTTGTGGATCGGTAGCTAATTGTAGGTTTTGGTCTTGGCTGTACTATTGTTTATGCTTATTTTTCATTGTTTTTCAATTTTTGAATGCTTTGTAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTACTCTCTATTACTCTAATTTGTACTTGGTTGATTAATTCGTATGGATGTTTGCATTCTTTATGGTCGTATGTTCCAAATGCTGACTTCTTATAATTCGTGTCGGTGGTGGAGTGAGGATTTGTTCTCCAAAATACTTTATGTTTGTTAAGATTGTGGATTGTTCACCAAGTACATTTTGTCATTAAGAAGTAACAAAACAAAACTACAAAAGAAGGGAGCCCTCAATAGGAGGATCTACAAAATACTTCTCCAATTAGAGAGCAGGAAAGGAAAGCTATAGTAATGAAAAAGAGGGGAGCATTTACGCCAAGTCACACAAACTAACTATTGTCCCAAAAATATCCTTAAAATTTTTTCTATTACGCTATTGGCAAATATCCTTAAAGAGCACTCACATAACGTTCCCGCAAGAAGCCTCGTTTCCTTTCTGAATGGATGACCCCTTGAACTTCAACTGTTTAAGCAATAAAGATCTTTGGATTCTCTGGTAGGGCCATATGCTAAAAAAACACCTTCATAAGAGCCAGGAGATTCAAATACTTCATTCCCTAAGGAGGACACTATGTCTTGGACCATTTTAAACTTTATTATAAGTTGATGCATGTGCATGTAAAGGTATCCGAGAGAACTTGATATTGAGGAGTTTGAAAATCCTTGAGGATGTCATTTTGTCCCAGACAAAGGTATTTGGAAAATGGGGAAAAGGGCGTCTTTTCTGAATGGTTCAGGTTTTCAAATAAGAAAAACTGAAAATATTCAGCCGGTGGTGAGCTTAGTCAAATTTGAACCAAATATACTCGAATCCGATCAAAGATTGAATAGTTTGCTAAGTAATGGAAGTGGTGCACCCGGTTAAAATTTTGCTTCCATATCCTAGAGGGCTTGAGTTCGATCCTCCGTCCTTGAGATTGGATCTTTTTAAAGACATTGGCGGCTTTCCTTCTGTTCAGCCAAATTTTTTCAGCACATCCCATTTGCAGTTCACAGGTTCTAAGCCCAGCCCAACGCTGTGTGCCATTAACCAAATGGTGTACTGCTTCTAATCACTAAACCAACAGTTTGGTAGGTTTGGTTATTATGTGTGACAATTCAGTTCGTTTTTTAAATTTCTTCCTAGATTTAGCAATTTACTTTTTGATACTCTAAACTAACAAAAACAATCTAGGATAATCTGAGTTCACTTGCTTTCCAAATGTGTTGCATACCAAATATGTTGATTGTGTTCATATTTTTCACTTAAGGTTCCTGAACGTCAATTGAGCAGTCAAGTAATTTTGTGCAGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACCTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACGACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACGAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCGAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGATTGCTTCCACACATAATAATTGATAGGTTTATGGTCTTAACTTAAAGATTCTTTGAGGAAATTAGATTGATACTGTTGTTGTACCACTAATATTCACATAAAATTGTCTTTCTTCAATCAGCTTGAGCACACAGTGAAGAAAGAAAAGATTTTGTATTTATTCTTGCGAATTGTAGCCTTATTTGATTTAAATAGGTAAGAGAGATAAGAGAGTAAACATTACCTTTTGGCTTTGCCCACATTATCTATTCTACCATCTTGTTTACATTCTTCTAGAAACATTTTTCATTGTGTTCAAATGTTCATAGTGATTTGATGCGAGACTCTTGAAAATGTAATTCTTCGACACGTTTCATTTTAGTTCAACAAATGTTCGGGGTGGATATTAAACTATTGACACCTTTGA

mRNA sequence

GATAACATAAAACAAAATTGATGATTTAAAACTGAGTTTGGCAAGATACAAAATGCTTGGAGGGAAAGATATTTACGTGAAAAACCTAATCCATAAGGCATAAACCCCGAAAGAAATAGATTCCATTTGCACCCTAAAATCTCCTCTACCGGACCGGCGGCGGCGATGGGCTGCCTAACCATCCTCATCCTTAGGTTATGAGATCTCTTTCCAATTCTCATCTTCATCTCAAATATATATTCTCTCTCCCTAACTCTCTCATGGCTTCCTCTCTTATACAAACCCTTAAACCAGATTTAGCTCCTCAAGACCTAGACGACCTTCTCTGTTCCCCTTCACAGAAACAGCCACAACAGGAATCCTCCTCTTTTACCAATTCCACTTCCAATGACTCAACTCCTCTCCTGGGGTCGCCGACTGGATATTTGAGTCGAGAGTCGCGCATTGAACGAGCCTGGGCTCACTGGACCAAGCTCGGTCGCCCAAAGTTGATTGTAGCTCCCATGGTTGATAATTCGGAGTTGCCATTTCGTTTACTCTGCAGGAAATATGGTGCTGAGGCTGCTTATACGCCTATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACCTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACGACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACGAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCGAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGATTGCTTCCACACATAATAATTGATAGGTTTATGGTCTTAACTTAAAGATTCTTTGAGGAAATTAGATTGATACTGTTGTTGTACCACTAATATTCACATAAAATTGTCTTTCTTCAATCAGCTTGAGCACACAGTGAAGAAAGAAAAGATTTTGTATTTATTCTTGCGAATTGTAGCCTTATTTGATTTAAATAGGTAAGAGAGATAAGAGAGTAAACATTACCTTTTGGCTTTGCCCACATTATCTATTCTACCATCTTGTTTACATTCTTCTAGAAACATTTTTCATTGTGTTCAAATGTTCATAGTGATTTGATGCGAGACTCTTGAAAATGTAATTCTTCGACACGTTTCATTTTAGTTCAACAAATGTTCGGGGTGGATATTAAACTATTGACACCTTTGA

Coding sequence (CDS)

ATGAGATCTCTTTCCAATTCTCATCTTCATCTCAAATATATATTCTCTCTCCCTAACTCTCTCATGGCTTCCTCTCTTATACAAACCCTTAAACCAGATTTAGCTCCTCAAGACCTAGACGACCTTCTCTGTTCCCCTTCACAGAAACAGCCACAACAGGAATCCTCCTCTTTTACCAATTCCACTTCCAATGACTCAACTCCTCTCCTGGGGTCGCCGACTGGATATTTGAGTCGAGAGTCGCGCATTGAACGAGCCTGGGCTCACTGGACCAAGCTCGGTCGCCCAAAGTTGATTGTAGCTCCCATGGTTGATAATTCGGAGTTGCCATTTCGTTTACTCTGCAGGAAATATGGTGCTGAGGCTGCTTATACGCCTATGCTCCATTCACGAATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTCACCACTTGTCAGGAGGACCGTCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTCTGTTGGAGGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTGTCCTCAGCGTATTGCAAGGCGAGGAAATTATGGGGCTTTTTTAATGGATAACCTACCTCTTGTAAAAGCAATAGTAGAGAAACTGGCTTCCAATCTTCAAGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATCTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGATGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAAAAATTCCGAGCTAACTGGAGTGCCATTAGTGCGGTTAAAAACGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATACGGCACATGGAAGATGTTAACGACTGTTTACAAGAGACGGGAGTTGAGGGTGTGCTCTCGGCAGAAACCCTTCTGGAGAACCCTGCACTTTTTGCTGGGTTTCGAACTGCTGAATGGATAGAGGGCCACGAAGAAAATACCCGGGATGGTAACCTGGATCAAGCGGATCTGCTTGTAGACTACTTGAAGCTTTGTGAAAAATACCCTGTCCCATGGAGAATGATCCGTTCGCATGTACACAAGTTATTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTCAATGCACAATCGAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAATTGCAGGTAAAAATGCCTCTTTATATCAAAGATTCTCATGCACTTGCAATTTCATCCAATGCTTGA

Protein sequence

MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTNSTSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA*

Homology

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BLAST of CSPI02G27070 vs. ExPASy Swiss-Prot
Match: Q8K582 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.0e-78
Identity = 142/298 (47.65%), Postives = 194/298 (65.10%), Query Frame = 0


Query: 90  WTK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 149
           W++ LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRALGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 150 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 209
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG YGAFL +   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGRYGAFLQEEWDLL 129

Query: 210 KAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 269
           + ++      L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTA 189

Query: 270 NWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAGFRTAE 329
           +W  I AV+ AV IPV ANGNIR ++DV  C+Q+TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVGIPVFANGNIRCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 330 WIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 385
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELADEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

[Close]

BLAST of CSPI02G27070 vs. ExPASy Swiss-Prot
Match: Q8C2P3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.7e-78
Identity = 141/298 (47.32%), Postives = 195/298 (65.44%), Query Frame = 0


Query: 90  WTK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 149
           W++ LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRTLGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 150 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 209
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG+YGAFL +   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQEEWDLL 129

Query: 210 KAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 269
           + ++      L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTA 189

Query: 270 NWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAGFRTAE 329
           +W  I AV+ AV IPV ANGNI+ ++DV  C+Q+TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVGIPVFANGNIQCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 330 WIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 385
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELAEEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

[Close]

BLAST of CSPI02G27070 vs. ExPASy Swiss-Prot
Match: Q6P1R4 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS1L PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 8.6e-78
Identity = 139/288 (48.26%), Postives = 189/288 (65.62%), Query Frame = 0


Query: 99  IVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTTCQEDRPLFVQ 158
           +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    C EDRPL VQ
Sbjct: 20  VVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCEVCPEDRPLIVQ 79

Query: 159 FCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASN 218
           FCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG+YGAFL D   L++ ++      
Sbjct: 80  FCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQDEWDLLQRMILLAHEK 139

Query: 219 LQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKN 278
           L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A+W  I AV+ 
Sbjct: 140 LSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPLSGAASWEHIKAVRK 199

Query: 279 AVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTR 338
           AV IPV ANGNI+ ++DV  CL++TGV+GV+SAE  L NPALF G   A W         
Sbjct: 200 AVAIPVFANGNIQCLQDVERCLRDTGVQGVMSAEGNLHNPALFEGRSPAVW--------- 259

Query: 339 DGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 385
                  +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 260 -------ELAEEYLDIVREHPCPLSYVRAHLFKLWHHTLQVHQELREE 291

[Close]

BLAST of CSPI02G27070 vs. ExPASy Swiss-Prot
Match: Q9HGN6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus1 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 5.6e-69
Identity = 137/302 (45.36%), Postives = 194/302 (64.24%), Query Frame = 0


Query: 90  WTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFT--TC 149
           + K+GRPK I+APMVD SELP+R+L R+ GA+  Y+PM HSR+F E+E YRN  F+  T 
Sbjct: 12  YNKIGRPKRILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFSTRTI 71

Query: 150 QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVK 209
            E+RPL +QFC NDP+++L+AA+   PYCD VD+NLGCPQ IA++G YG+FL +N  L++
Sbjct: 72  PEERPLIIQFCGNDPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQENWNLIE 131

Query: 210 AIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRAN 269
           +I+ KL + L +PV+ KIRIFP+ Q T++YA+M+  AG S+LAVHGR R++K      A+
Sbjct: 132 SIITKLHTELSIPVTAKIRIFPDPQKTLDYAKMILKAGASILAVHGRLREQKGHFTGIAD 191

Query: 270 WSAISAV-KNAVRIPVL-ANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAGFRTA 329
           W  I  + KN     VL ANGNI H +D++ C++ TGV+GVLSAE  L NP +F      
Sbjct: 192 WEQIQMLRKNLPSETVLFANGNILHAQDIDRCIKYTGVDGVLSAEGSLYNPRIFLP---- 251

Query: 330 EWIEGHEENTRDGNLDQADLLVDYLKLCEKYPV-----PWRMIRSHVHKLLGDWFKIHPH 383
                    T    +D  D+  +YL +  ++ +         I+ H+ KL+     IH  
Sbjct: 252 ---PSSPLMTLYPRID--DMCEEYLNIIREFKLESDYSSLSAIKGHLFKLMRPLLSIHTD 304

[Close]

BLAST of CSPI02G27070 vs. ExPASy Swiss-Prot
Match: P53759 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DUS1 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.4e-64
Identity = 130/313 (41.53%), Postives = 189/313 (60.38%), Query Frame = 0


Query: 92  KLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTT---CQ 151
           K+GRP  IVAPMVD SEL +R+L R+YGA  AYTPMLH+++F  ++KYR   +++     
Sbjct: 25  KIGRPTRIVAPMVDQSELAWRILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDGSS 84

Query: 152 EDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKA 211
            DRPL VQFCANDP+ LL AA+ VE  CD VDLNLGCPQ IA++G+YG+FLM+   L+  
Sbjct: 85  VDRPLVVQFCANDPEYLLAAAKLVEDKCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLIHN 144

Query: 212 IVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANW 271
           ++  L  NL+VPV+ KIRIF + + ++NYA+M+ DAG   L VHGR R++K  K   ANW
Sbjct: 145 LINTLHKNLKVPVTAKIRIFDDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTGLANW 204

Query: 272 SAISAVKNAV--RIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAGFRTAE 331
             I  +++ +       ANGNI + ED++ C++  G + V+SAE  L NP +F   +T  
Sbjct: 205 ETIKYLRDNLPKETVFFANGNILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVGQT-- 264

Query: 332 WIEGHEENTRDGNLDQADLLV----DYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIR 391
                    ++    + D ++      +K C++       ++SH  K+L  +   H  IR
Sbjct: 265 -------KNKEKIFPRVDKIIREYFQIVKECQESKASKTAMKSHFFKILRPFLPHHTDIR 324

Query: 392 EDF-NAQSKLTFE 395
                  +K T+E
Sbjct: 325 STLATMNAKATWE 328

[Close]

BLAST of CSPI02G27070 vs. ExPASy TrEMBL
Match: A0A0A0LTG2 (Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 9.1e-248
Identity = 423/426 (99.30%), Postives = 423/426 (99.30%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPS KQPQ ESSS TN
Sbjct: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTN 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420

Query: 421 AISSNA 427
           AISSNA
Sbjct: 421 AISSNA 426

[Close]

BLAST of CSPI02G27070 vs. ExPASy TrEMBL
Match: A0A1S3CHU6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=LOC103501114 PE=4 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 9.7e-234
Identity = 404/426 (94.84%), Postives = 407/426 (95.54%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRSLSNSHL+LK IFSL NS MASS  QTLKPDL PQDLDDLLCS  QKQ Q ESSS T 
Sbjct: 1   MRSLSNSHLYLKTIFSLRNSFMASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTG 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           S SNDS  LLGSPTGYLS ESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  SNSNDSKLLLGSPTGYLSGESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           +AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 DAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVN+CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEWIEG EENTRDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSH  
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVP 420

Query: 421 AISSNA 427
           AISSNA
Sbjct: 421 AISSNA 426

[Close]

BLAST of CSPI02G27070 vs. ExPASy TrEMBL
Match: A0A6J1FWR0 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111448913 PE=4 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.3e-217
Identity = 382/424 (90.09%), Postives = 394/424 (92.92%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRS SNSHLHLK I SL  S MASS  QT   D  PQDLDDLLCS    QPQ E SS T 
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DF-PQDLDDLLCSDPHTQPQDECSSLTK 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SNDST LLGSP+GYLS ESRIERAWAHW+KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  --SNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV++CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTA+W  G EE++RDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVK+PLYIKDS A 
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPAS 418

Query: 421 AISS 425
           AISS
Sbjct: 421 AISS 418

[Close]

BLAST of CSPI02G27070 vs. ExPASy TrEMBL
Match: A0A6J1JGU3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111484251 PE=4 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.4e-216
Identity = 381/424 (89.86%), Postives = 392/424 (92.45%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRS SNSHLHLK I SL  S MASS  QT   D  PQDLDDLLCS    Q Q E SS T 
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSCAQT---DF-PQDLDDLLCSDPHTQRQDECSSLTK 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SNDST LLGSP+GYL+ ESRIERAWAHW+KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  --SNDSTLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAI AVKNA+RIPVLANGNIRHMEDVN+CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTA+W  G EE++RDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDS A 
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAS 418

Query: 421 AISS 425
           AISS
Sbjct: 421 AISS 418

[Close]

BLAST of CSPI02G27070 vs. ExPASy TrEMBL
Match: A0A6J1D4R6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3673 GN=LOC111017278 PE=4 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.3e-214
Identity = 370/425 (87.06%), Postives = 398/425 (93.65%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRSL+ SHLHLK +FSL +SLMASS  QT+KPDL P+ LDDLLCS  Q+Q ++E SS T 
Sbjct: 1   MRSLAISHLHLKSVFSLHSSLMASSFSQTIKPDL-PEHLDDLLCSNQQQQQREECSSVTK 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             S++ + LLGSP GYLS +SRIERAWAHW+KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  --SDEPSLLLGSPNGYLSGDSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIV+KL+SNLQVPVSCKIRIFPNLQDT+NYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVQKLSSNLQVPVSCKIRIFPNLQDTLNYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAV+IPVLANGNIRHMEDVN+CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVQIPVLANGNIRHMEDVNNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEW+EG +EN RDGNLDQA LLV+YLKLCE+YPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWVEGCDENCRDGNLDQAALLVEYLKLCEQYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           R+IRSHVHK+LGDWFKIHPH+REDFNAQ KLTFEFLY MV+RLRELQVK+PLY+KDS A 
Sbjct: 361 RIIRSHVHKMLGDWFKIHPHVREDFNAQFKLTFEFLYIMVDRLRELQVKIPLYLKDSPAS 420

Query: 421 AISSN 426
            ISSN
Sbjct: 421 EISSN 422

[Close]

BLAST of CSPI02G27070 vs. NCBI nr
Match: XP_011650036.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis sativus])

HSP 1 Score: 865.5 bits (2235), Expect = 1.9e-247
Identity = 423/426 (99.30%), Postives = 423/426 (99.30%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPS KQPQ ESSS TN
Sbjct: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTN 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420

Query: 421 AISSNA 427
           AISSNA
Sbjct: 421 AISSNA 426

[Close]

BLAST of CSPI02G27070 vs. NCBI nr
Match: KAE8652323.1 (hypothetical protein Csa_021950 [Cucumis sativus])

HSP 1 Score: 826.2 bits (2133), Expect = 1.3e-235
Identity = 402/405 (99.26%), Postives = 402/405 (99.26%), Query Frame = 0


Query: 22  MASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTNSTSNDSTPLLGSPTGYLSRES 81
           MASSLIQTLKPDLAPQDLDDLLCSPS KQPQ ESSS TNSTSNDSTPLLGSPTGYLSRES
Sbjct: 1   MASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTNSTSNDSTPLLGSPTGYLSRES 60

Query: 82  RIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRN 141
           RIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRN
Sbjct: 61  RIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRN 120

Query: 142 MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMD 201
           MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMD
Sbjct: 121 MEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMD 180

Query: 202 NLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDG 261
           NLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDG
Sbjct: 181 NLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDG 240

Query: 262 KKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAG 321
           KKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAG
Sbjct: 241 KKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFAG 300

Query: 322 FRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHI 381
           FRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHI
Sbjct: 301 FRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHI 360

Query: 382 REDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 427
           REDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA
Sbjct: 361 REDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHALAISSNA 405

[Close]

BLAST of CSPI02G27070 vs. NCBI nr
Match: XP_008462837.1 (PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis melo])

HSP 1 Score: 818.9 bits (2114), Expect = 2.0e-233
Identity = 404/426 (94.84%), Postives = 407/426 (95.54%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRSLSNSHL+LK IFSL NS MASS  QTLKPDL PQDLDDLLCS  QKQ Q ESSS T 
Sbjct: 1   MRSLSNSHLYLKTIFSLRNSFMASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTG 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
           S SNDS  LLGSPTGYLS ESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  SNSNDSKLLLGSPTGYLSGESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           +AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 DAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVN+CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEWIEG EENTRDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSH  
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVP 420

Query: 421 AISSNA 427
           AISSNA
Sbjct: 421 AISSNA 426

[Close]

BLAST of CSPI02G27070 vs. NCBI nr
Match: XP_038901742.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida])

HSP 1 Score: 789.6 bits (2038), Expect = 1.3e-224
Identity = 392/426 (92.02%), Postives = 399/426 (93.66%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRS  NSHLH K IFS  +SLMASS  QTLKPDL PQDLDD LCS  Q Q QQE SSFT 
Sbjct: 1   MRSPYNSHLHFKTIFSFRSSLMASSFTQTLKPDL-PQDLDDRLCSDPQNQQQQECSSFTK 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SNDST LL SP+GYL  ESRIERAWAHW KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  --SNDSTLLLASPSGYLCGESRIERAWAHWRKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIF NLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFSNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVR+PVLANGNIRHMEDV +CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRVPVLANGNIRHMEDVYNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTAEWI+GHEENTRDGNLDQADLLVDYLK CEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIKGHEENTRDGNLDQADLLVDYLKFCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDSHA 
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHAS 420

Query: 421 AISSNA 427
           AI+SNA
Sbjct: 421 AIASNA 423

[Close]

BLAST of CSPI02G27070 vs. NCBI nr
Match: XP_022944464.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata])

HSP 1 Score: 765.4 bits (1975), Expect = 2.6e-217
Identity = 382/424 (90.09%), Postives = 394/424 (92.92%), Query Frame = 0


Query: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSQKQPQQESSSFTN 60
           MRS SNSHLHLK I SL  S MASS  QT   D  PQDLDDLLCS    QPQ E SS T 
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DF-PQDLDDLLCSDPHTQPQDECSSLTK 60

Query: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120
             SNDST LLGSP+GYLS ESRIERAWAHW+KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  --SNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV++CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTA+W  G EE++RDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVK+PLYIKDS A 
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPAS 418

Query: 421 AISS 425
           AISS
Sbjct: 421 AISS 418

[Close]

BLAST of CSPI02G27070 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 609.0 bits (1569), Expect = 2.9e-174
Identity = 297/400 (74.25%), Postives = 339/400 (84.75%), Query Frame = 0


Query: 20  SLMASSLIQTLKPDLAP---QDLDDLLCSPSQKQPQQESSSFTNSTSNDSTPLLGSPTGY 79
           SL++ +   T  PD  P   Q LDD+LCS  Q+  Q E +  T   S      LGSP+  
Sbjct: 16  SLISQTRAMTQNPDPKPDPSQVLDDILCS-EQRDGQIEETVDTAPAS------LGSPSRV 75

Query: 80  LSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTEN 139
           LS ++R+ERAWAHW KLGRPK IVAPMVDNSELPFRLLC+KYGA+AAYTPMLHSRIFTE 
Sbjct: 76  LSIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTET 135

Query: 140 EKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYG 199
           EKYRN EFTTC+EDRPLFVQFCANDPD LLEAA+RVEPYCDYVD+NLGCPQRIARRGNYG
Sbjct: 136 EKYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYG 195

Query: 200 AFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTR 259
           AFLMDNLPLVK++VEKLA NL VPVSCKIRIFPNL+DT+ YA+MLEDAGCSLLAVHGRTR
Sbjct: 196 AFLMDNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTR 255

Query: 260 DEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENP 319
           DEKDGKKFRA+WSAI  VKNA+RIPVLANGN+R +EDV++C++ETGVEGVLSAETLLENP
Sbjct: 256 DEKDGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLLENP 315

Query: 320 ALFAGFRTAEWI-EGHEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWF 379
           A FAGFRTAEW  +  EE   DG LDQ DL+V+YLKLCEK+PVPWRMIRSHVHK+LG+WF
Sbjct: 316 AAFAGFRTAEWAKDNEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRSHVHKMLGEWF 375

Query: 380 KIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIK 416
           +IHP +RE  NAQ+ LTFEFLY +V++LREL  ++PLY K
Sbjct: 376 RIHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKK 408

[Close]

BLAST of CSPI02G27070 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-29
Identity = 79/247 (31.98%), Postives = 122/247 (49.39%), Query Frame = 0


Query: 97  KLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNME------------- 156
           KL++APMV    L FR+L  +YGA+  Y   +      + E+  N+              
Sbjct: 6   KLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVEKGTDN 65

Query: 157 --FTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLM 216
             F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L+
Sbjct: 66  VVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAALL 125

Query: 217 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 276
               L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   D
Sbjct: 126 SKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIAD 185

Query: 277 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALFA 328
             +  A W  I+ V  A+ IPV+ANG++   +D +     TG   V+ A   + N ++F+
Sbjct: 186 RPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIFS 245

[Close]

BLAST of CSPI02G27070 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-24
Identity = 64/188 (34.04%), Postives = 99/188 (52.66%), Query Frame = 0


Query: 141 NMEFTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFL 200
           N+ F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L
Sbjct: 26  NVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAAL 85

Query: 201 MDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEK 260
           +    L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   
Sbjct: 86  LSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIA 145

Query: 261 DGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQETGVEGVLSAETLLENPALF 320
           D  +  A W  I+ V  A+ IPV+ANG++   +D +     TG   V+ A   + N ++F
Sbjct: 146 DRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIF 205

Query: 321 AGFRTAEW 328
           +    + W
Sbjct: 206 SPKGKSHW 211

[Close]

BLAST of CSPI02G27070 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 7.3e-16
Identity = 54/207 (26.09%), Postives = 95/207 (45.89%), Query Frame = 0


Query: 95  RPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 154
           R KL +AP+     LPFR LC+  GA+     M       + +            +    
Sbjct: 337 RDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFG 396

Query: 155 VQFCANDPDVLLEAARRVEPYC--DYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK 214
           VQ C + PD +      ++  C  D++D+N+GCP  +    + G+ L++    +K IVE 
Sbjct: 397 VQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVEV 456

Query: 215 LASNLQVPVSCKIR--IFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSA 274
            +S ++ P++ K+R   F       +    + + G + + +HGR+R ++  K   A+W  
Sbjct: 457 SSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKS--ADWDY 516

Query: 275 I-SAVKNA-VRIPVLANGNIRHMEDVN 296
           I    KNA   + V+ NG++    D N
Sbjct: 517 IYQCTKNATTNLQVIGNGDVYSYLDWN 541

[Close]

BLAST of CSPI02G27070 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 9.3e-11
Identity = 67/249 (26.91%), Postives = 98/249 (39.36%), Query Frame = 0


Query: 96  PKLIVAPMVDNSELPFRLLCRKYGAEA-AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 155
           P   VAPM+D ++  +R L R     A  YT M+ +      +   +       +  P+ 
Sbjct: 52  PSFSVAPMMDWTDNHYRTLARLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHPIV 111

Query: 156 VQFCANDPDVLLEAARRVEPY-CDYVDLNLGCPQ-RIARRGNYGAFLMDNLPLVKAIVEK 215
           +Q   ++ + L +AA+  + Y  D ++LN GCP  ++A  G +G  LM    LV   +  
Sbjct: 112 LQLGGSNVENLAKAAKLSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAMSA 171

Query: 216 LASNLQVPVSCKIRI-------FPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFR 275
           +A+N  VPV+ K RI       +  L D I     L         VH R          +
Sbjct: 172 IAANTNVPVTVKCRIGVDNHDSYDELCDFIYKVSTLSPT--RHFIVHSR----------K 231

Query: 276 ANWSAISAVKNAVRIPVL------------------ANGNIRHMEDVNDCLQETGVEGVL 317
           A    IS   N  RIP L                   NG I  +  VN  L+E G  GV+
Sbjct: 232 ALLGGISPADNR-RIPPLKYEYYYALVRDFPDLRFTINGGITSVSKVNAALKE-GAHGVM 286

The following BLAST results are available for this feature:

BLAST of CSPI02G27070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8K582	1.0e-78	47.65	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=101...	[more]
Q8C2P3	1.7e-78	47.32	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN...	[more]
Q6P1R4	8.6e-78	48.26	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=...	[more]
Q9HGN6	5.6e-69	45.36	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (st...	[more]
P53759	2.4e-64	41.53	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (str...	[more]

BLAST of CSPI02G27070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTG2	9.1e-248	99.30	Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV...	[more]
A0A1S3CHU6	9.7e-234	94.84	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1FWR0	1.3e-217	90.09	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1JGU3	1.4e-216	89.86	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1D4R6	1.3e-214	87.06	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3...	[more]

BLAST of CSPI02G27070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650036.1	1.9e-247	99.30	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis sativus]	[more]
KAE8652323.1	1.3e-235	99.26	hypothetical protein Csa_021950 [Cucumis sativus]	[more]
XP_008462837.1	2.0e-233	94.84	PREDICTED: tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucumis melo]	[more]
XP_038901742.1	1.3e-224	92.02	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida]	[more]
XP_022944464.1	2.6e-217	90.09	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata]	[more]

BLAST of CSPI02G27070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67220.1	2.9e-174	74.25	FMN-linked oxidoreductases superfamily protein	[more]
AT3G49640.1	1.2e-29	31.98	Aldolase-type TIM barrel family protein	[more]
AT3G49640.2	1.5e-24	34.04	Aldolase-type TIM barrel family protein	[more]
AT4G38890.1	7.3e-16	26.09	FMN-linked oxidoreductases superfamily protein	[more]
AT3G63510.1	9.3e-11	26.91	FMN-linked oxidoreductases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035587	DUS-like, FMN-binding domain	PFAM	PF01207	Dus	coord: 99..390 e-value: 6.8E-74 score: 248.8
IPR035587	DUS-like, FMN-binding domain	CDD	cd02801	DUS_like_FMN	coord: 97..327 e-value: 8.41516E-107 score: 313.277
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 95..328 e-value: 5.1E-80 score: 270.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..75
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 43..74
None	No IPR available	PANTHER	PTHR11082	TRNA-DIHYDROURIDINE SYNTHASE	coord: 77..415
None	No IPR available	PANTHER	PTHR11082:SF35	BNAA09G07510D PROTEIN	coord: 77..415
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 96..372
IPR018517	tRNA-dihydrouridine synthase, conserved site	PROSITE	PS01136	UPF0034	coord: 179..197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G27070.1	CSPI02G27070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002943	tRNA dihydrouridine synthesis
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0017150	tRNA dihydrouridine synthase activity
molecular_function	GO:0003824	catalytic activity