CSPI02G23910 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G23910
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionWRKY transcription factor WRKY76-like
LocationChr2: 20546143 .. 20547522 (+)
RNA-Seq ExpressionCSPI02G23910
SyntenyCSPI02G23910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCTCTTTCTCTCCATAGAAGCATTCATATGGATTCAAAGGCAAGTTTCTTCTTACATAAAATCATCGTGAAGAGTATAATATTAATTATATGAATTAATTACAAAAGATTTTTGCACTATAACACTATATATATTTCAGGTGGAATGTCTCCAAACAGAGCTAGAAAAGTTGAGGAAAGAGAATGAAGCTCTGAAGTTGATGCTGAAAGTTGTGAGCATCAAGAACTTGGTATCTCAAGTGGATGTTTGTTCAAGAGATCATCGTCATGATCAGAATGACGAGGGTTCAAGATCAGAGAGAGCCAATCTCAAAGTTAGTCCACCCGCTCTTGCTCTTGCTCTCGAAACTTCGTCCACCACCCAAGCATATGCTAGAACTACCTTCAAAGACCAAGCTTTGGTATGGAAATTTAATAGTAGAAATCAGTTTTCCTGTGATCTCCCTCTTCTTTTGTTTTTAATGAAAAATTTCGATGCCATGTATTTATTTGGGTTGAATTATTAAGATGGTGAAGGATGGATATAAATGGAGGAAATATGGGCAAAAGATTACCAAGGATAATCAATCTCCTCGTGCTTATTTCAAGTGTTCTTCCCCAGGATGCCCTGTCAAAAAAAAGGTAATTTATTCCAATTTCTAAATTACAAAGTTTTAAATTTGAAATTTTACTATGTTTTGATTCATTACACTATAATGGTATCAATTTAATGTTCTTACTCTTATTTAGTTTGAAAGTTATATATTAGATTACAATTGTAATTGTAGTATACCTAAATAAAATTAAAGAGAATTTTGACATATGACAAATTTTTAGTATGCAACATCTGTAAGTTGCAACTTGAAACTCAAAAAAAAAAAAATTCTTTAAGAAACATGCCATTGTAAATGTGGAAAATAGAATTAAAATGTTTCATTTGTATAAGTAAAGTGCAAAAAGATTATTGTCATCTCCATCTAACTTACATTATAAAACCATATTTGGAAAATAGAAGAAAACTAATAAGACCATGATGATGATCAGAATTCAGAATTCAGTATCTTGGGGATTACATTAAATGATTTCTCCTATACTTTTTAGTTTTCTATAATAATCAATGGAATTGTTATAAATAGTTAATAGAATTAAAATGTGAAATGGTGTTGAAATTTAGGTACAAAGAAGCCTGGAAAATAAGTCAATGGTGATAGTCACATACGATGGCCACCACAACCACAACCACAACCACGAAAATGCCTCTCCTCCACCGCTTTCAAGCTCTCAACGAGGGTCGTCTTCGTCACCACCGCTTCCGGTGGAGACCAACCGAGTTGCTCTACCAATGTCTCTCAATCTTGATCTCACTCTTTCCAGACATGCAGATGATCATAAATTATAA

mRNA sequence

CTTTCTCTTTCTCTCCATAGAAGCATTCATATGGATTCAAAGGTGGAATGTCTCCAAACAGAGCTAGAAAAGTTGAGGAAAGAGAATGAAGCTCTGAAGTTGATGCTGAAAGTTGTGAGCATCAAGAACTTGGTATCTCAAGTGGATGTTTGTTCAAGAGATCATCGTCATGATCAGAATGACGAGGGTTCAAGATCAGAGAGAGCCAATCTCAAAGTTAGTCCACCCGCTCTTGCTCTTGCTCTCGAAACTTCGTCCACCACCCAAGCATATGCTAGAACTACCTTCAAAGACCAAGCTTTGATGGTGAAGGATGGATATAAATGGAGGAAATATGGGCAAAAGATTACCAAGGATAATCAATCTCCTCGTGCTTATTTCAAGTGTTCTTCCCCAGGATGCCCTGTCAAAAAAAAGGTACAAAGAAGCCTGGAAAATAAGTCAATGGTGATAGTCACATACGATGGCCACCACAACCACAACCACAACCACGAAAATGCCTCTCCTCCACCGCTTTCAAGCTCTCAACGAGGGTCGTCTTCGTCACCACCGCTTCCGGTGGAGACCAACCGAGTTGCTCTACCAATGTCTCTCAATCTTGATCTCACTCTTTCCAGACATGCAGATGATCATAAATTATAA

Coding sequence (CDS)

ATGGATTCAAAGGTGGAATGTCTCCAAACAGAGCTAGAAAAGTTGAGGAAAGAGAATGAAGCTCTGAAGTTGATGCTGAAAGTTGTGAGCATCAAGAACTTGGTATCTCAAGTGGATGTTTGTTCAAGAGATCATCGTCATGATCAGAATGACGAGGGTTCAAGATCAGAGAGAGCCAATCTCAAAGTTAGTCCACCCGCTCTTGCTCTTGCTCTCGAAACTTCGTCCACCACCCAAGCATATGCTAGAACTACCTTCAAAGACCAAGCTTTGATGGTGAAGGATGGATATAAATGGAGGAAATATGGGCAAAAGATTACCAAGGATAATCAATCTCCTCGTGCTTATTTCAAGTGTTCTTCCCCAGGATGCCCTGTCAAAAAAAAGGTACAAAGAAGCCTGGAAAATAAGTCAATGGTGATAGTCACATACGATGGCCACCACAACCACAACCACAACCACGAAAATGCCTCTCCTCCACCGCTTTCAAGCTCTCAACGAGGGTCGTCTTCGTCACCACCGCTTCCGGTGGAGACCAACCGAGTTGCTCTACCAATGTCTCTCAATCTTGATCTCACTCTTTCCAGACATGCAGATGATCATAAATTATAA

Protein sequence

MDSKVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETNRVALPMSLNLDLTLSRHADDHKL*
Homology
BLAST of CSPI02G23910 vs. ExPASy Swiss-Prot
Match: Q6IEL0 (WRKY transcription factor WRKY71 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY71 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-22
Identity = 58/127 (45.67%), Postives = 77/127 (60.63%), Query Frame = 0

Query: 78  TQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGCPVKKKVQRSLEN 137
           ++ Y      D +L+VKDGY+WRKYGQK+TKDN  PRAYF+CS +P CPVKKKVQRS E+
Sbjct: 177 SKLYVHADPSDLSLVVKDGYQWRKYGQKVTKDNPCPRAYFRCSFAPACPVKKKVQRSAED 236

Query: 138 KSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSS--------------PPLPVETNRV 190
            ++++ TY+G HNH        PPPL S+ + S  S              PP PV  +R 
Sbjct: 237 NTILVATYEGEHNHGQ-----PPPPLQSAAQNSDGSGKSAGKPPHAPAAAPPAPVVPHRQ 296

BLAST of CSPI02G23910 vs. ExPASy Swiss-Prot
Match: Q6QHD1 (WRKY transcription factor WRKY71 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY71 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-22
Identity = 58/127 (45.67%), Postives = 77/127 (60.63%), Query Frame = 0

Query: 78  TQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGCPVKKKVQRSLEN 137
           ++ Y      D +L+VKDGY+WRKYGQK+TKDN  PRAYF+CS +P CPVKKKVQRS E+
Sbjct: 177 SKLYVHADPSDLSLVVKDGYQWRKYGQKVTKDNPCPRAYFRCSFAPACPVKKKVQRSAED 236

Query: 138 KSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSS--------------PPLPVETNRV 190
            ++++ TY+G HNH        PPPL S+ + S  S              PP PV  +R 
Sbjct: 237 NTILVATYEGEHNHGQ-----PPPPLQSAAQNSDGSGKSAGKPPHAPAAAPPAPVVPHRQ 296

BLAST of CSPI02G23910 vs. ExPASy Swiss-Prot
Match: Q9SK33 (Probable WRKY transcription factor 60 OS=Arabidopsis thaliana OX=3702 GN=WRKY60 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.2e-22
Identity = 72/174 (41.38%), Postives = 101/174 (58.05%), Query Frame = 0

Query: 2   DSKVECLQTELEKLRKENEALKLML-----KVVSIKNLVSQVDV-CSRDHRHDQNDE--G 61
           D K   LQ E+ ++  EN+ L  ML     K  ++ NL+ ++    S +  + QN +  G
Sbjct: 39  DEKRNMLQDEINRVNSENKKLTEMLARVCEKYYALNNLMEELQSRKSPESVNFQNKQLTG 98

Query: 62  SRSERANLKVSPPALALAL--------ETSSTTQAYARTTFKDQALMVKDGYKWRKYGQK 121
            R +  +  VS P + L+L        + ++ + AY      D +L VKDGY+WRKYGQK
Sbjct: 99  KRKQELDEFVSSP-IGLSLGPIENITNDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQK 158

Query: 122 ITKDNQSPRAYFKCS-SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENAS 159
           IT+DN SPRAYF+CS SP C VKKKVQRS E+ S ++ TY+G HNH   H + S
Sbjct: 159 ITRDNPSPRAYFRCSFSPSCLVKKKVQRSAEDPSFLVATYEGTHNHTGPHASVS 211

BLAST of CSPI02G23910 vs. ExPASy Swiss-Prot
Match: Q6EPZ2 (WRKY transcription factor WRKY76 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY76 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.2e-22
Identity = 54/107 (50.47%), Postives = 73/107 (68.22%), Query Frame = 0

Query: 88  DQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGCPVKKKVQRSLENKSMVIVTYDG 147
           D +L+VKDGY+WRKYGQK+T+DN SPRAYF+C+ +P CPVKKKVQRS E+ S+++ TY+G
Sbjct: 160 DTSLVVKDGYQWRKYGQKVTRDNPSPRAYFRCAFAPSCPVKKKVQRSAEDSSLLVATYEG 219

Query: 148 HHNHNHNHENASPPPLSSSQRGSSSSPPLPVETNRVALPMSLNLDLT 194
            HNH H    A   P ++   G S    LP   +  +   ++ LDLT
Sbjct: 220 EHNHPHPSPRAGELPAAAGGAGGS----LPCSISINSSGPTITLDLT 262

BLAST of CSPI02G23910 vs. ExPASy Swiss-Prot
Match: Q6IEK5 (WRKY transcription factor WRKY76 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY76 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 9.3e-22
Identity = 54/107 (50.47%), Postives = 72/107 (67.29%), Query Frame = 0

Query: 88  DQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGCPVKKKVQRSLENKSMVIVTYDG 147
           D +L+VKDGY+WRKYGQK+T+DN SPRAYF+C+ +P CPVKKKVQRS E+ S+++ TY+G
Sbjct: 160 DTSLVVKDGYQWRKYGQKVTRDNPSPRAYFRCAFAPSCPVKKKVQRSAEDSSLLVATYEG 219

Query: 148 HHNHNHNHENASPPPLSSSQRGSSSSPPLPVETNRVALPMSLNLDLT 194
            HNH H    A   P +    G S    LP   +  +   ++ LDLT
Sbjct: 220 EHNHPHPSPRAGELPAAVGGAGGS----LPCSISINSSGPTITLDLT 262

BLAST of CSPI02G23910 vs. ExPASy TrEMBL
Match: A0A0A0LMJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G382660 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.5e-107
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MDSKVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERAN 60
           MDSKVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERAN
Sbjct: 1   MDSKVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERAN 60

Query: 61  LKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS 120
           LKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS
Sbjct: 61  LKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS 120

Query: 121 SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETN 180
           SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETN
Sbjct: 121 SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETN 180

Query: 181 RVALPMSLNLDLTLSRHADDHKL 204
           RVALPMSLNLDLTLSRHADDHKL
Sbjct: 181 RVALPMSLNLDLTLSRHADDHKL 203

BLAST of CSPI02G23910 vs. ExPASy TrEMBL
Match: A0A1S4DVG5 (probable WRKY transcription factor 40 OS=Cucumis melo OX=3656 GN=LOC103488348 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.0e-79
Identity = 160/175 (91.43%), Postives = 166/175 (94.86%), Query Frame = 0

Query: 25  MLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSPPALALALETSSTTQAYART 84
           MLKVVSIKNLVSQVDVCSRD  HDQNDEGSRSERA+L+VSPP     LETSSTTQAYA T
Sbjct: 1   MLKVVSIKNLVSQVDVCSRD--HDQNDEGSRSERADLRVSPP----TLETSSTTQAYATT 60

Query: 85  TFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTY 144
           +FKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKK+VQRSLENKSMVIVTY
Sbjct: 61  SFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKEVQRSLENKSMVIVTY 120

Query: 145 DGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETNRVALPMSLNLDLTLSRHAD 200
           DGHHNHNHNHENASPPPLS+SQRG SSSPPLPVE+NRVALPMSLNLDLTLSRHAD
Sbjct: 121 DGHHNHNHNHENASPPPLSASQRG-SSSPPLPVESNRVALPMSLNLDLTLSRHAD 168

BLAST of CSPI02G23910 vs. ExPASy TrEMBL
Match: A0A5A7VHE4 (Putative WRKY transcription factor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002030 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 5.0e-79
Identity = 162/177 (91.53%), Postives = 166/177 (93.79%), Query Frame = 0

Query: 25  MLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSPPALALALETSSTTQAYART 84
           MLKVVSIKNLVSQVDVCSRD  HDQNDEGSRSERA+LKVSPP     LETSSTTQAYA T
Sbjct: 1   MLKVVSIKNLVSQVDVCSRD--HDQNDEGSRSERADLKVSPP----TLETSSTTQAYATT 60

Query: 85  TFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTY 144
           +FKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTY
Sbjct: 61  SFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTY 120

Query: 145 DGH--HNHNHNHENASPPPLSSSQRGSSSSPPLPVETNRVALPMSLNLDLTLSRHAD 200
           DGH  HNHNHNHENASPPPLS+SQRG SSSPPLPVE+NRVALPMSLNLDLTLSRHAD
Sbjct: 121 DGHHNHNHNHNHENASPPPLSASQRG-SSSPPLPVESNRVALPMSLNLDLTLSRHAD 170

BLAST of CSPI02G23910 vs. ExPASy TrEMBL
Match: A0A6J1BS37 (WRKY transcription factor WRKY76-like OS=Momordica charantia OX=3673 GN=LOC111005051 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 4.8e-37
Identity = 111/205 (54.15%), Postives = 128/205 (62.44%), Query Frame = 0

Query: 4   KVECLQTELEKLRKENEALKLMLKVVSIKNLV--------SQVDVCSRDHRHDQNDEGSR 63
           KVECLQ ELE+LRK NEAL   L V+S+KNL+        SQV V       D N   ++
Sbjct: 10  KVECLQKELERLRKANEALNFALGVMSMKNLIISRETNTNSQVGVFLEG--SDLNCNSNK 69

Query: 64  SERANLKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRA 123
             RA+L+         L   STTQ Y RT FKDQALM KDGY+WRKYGQKITKDNQ PRA
Sbjct: 70  RARADLQA-----RAGLGAPSTTQTYVRTGFKDQALMAKDGYRWRKYGQKITKDNQYPRA 129

Query: 124 YFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPP- 183
           YF CSS GCPVKKKVQRSLE+KSMV+V+Y       HNHE  SP   S+    S SSP  
Sbjct: 130 YFNCSSRGCPVKKKVQRSLEDKSMVMVSY----QDQHNHECDSPSHGSTRPFLSRSSPVV 189

Query: 184 ---LPVETNRVALPM-SLNLDLTLS 196
              LP       +P+ SL+LDLTL+
Sbjct: 190 VHCLPSTPCNATVPLVSLDLDLTLA 203

BLAST of CSPI02G23910 vs. ExPASy TrEMBL
Match: A0A6P4A722 (probable WRKY transcription factor 40 OS=Ziziphus jujuba OX=326968 GN=LOC107419756 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 6.0e-32
Identity = 89/199 (44.72%), Postives = 129/199 (64.82%), Query Frame = 0

Query: 4   KVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRH---DQNDEGSRSERAN 63
           K E LQ +LE +RKENE+L+ ML+V++ K  + +  +  R+  +   D N +   ++RA 
Sbjct: 10  KAEALQEDLECMRKENESLRFMLEVMTRKCNIIESHLRQRNLEYMGIDLNHQFEANKRAR 69

Query: 64  LKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS 123
             ++    +LA   S T+Q   RT  KD  L+VKDGY+WRKYGQK+TKDN SPRAYF+CS
Sbjct: 70  TSIN--EFSLATNKSQTSQFLVRTNSKDSTLVVKDGYQWRKYGQKVTKDNPSPRAYFRCS 129

Query: 124 -SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENAS-PPPLSSSQRGSSSSPPLPVE 183
            +PGCPVKKKVQRS ++KS++I TY+G HNH+ +  +A   P +++S +G+     +P E
Sbjct: 130 MAPGCPVKKKVQRSKDDKSVLIATYEGQHNHDSHDSHAHFSPSINTSAKGAEHINFVPAE 189

Query: 184 TNR--VALPMSLNLDLTLS 196
           +       P  + LDLTLS
Sbjct: 190 STPTFTPSPQPITLDLTLS 206

BLAST of CSPI02G23910 vs. NCBI nr
Match: XP_004138843.1 (probable WRKY transcription factor 60 [Cucumis sativus] >KGN62993.1 hypothetical protein Csa_022585 [Cucumis sativus])

HSP 1 Score: 398.7 bits (1023), Expect = 3.1e-107
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MDSKVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERAN 60
           MDSKVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERAN
Sbjct: 1   MDSKVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERAN 60

Query: 61  LKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS 120
           LKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS
Sbjct: 61  LKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS 120

Query: 121 SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETN 180
           SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETN
Sbjct: 121 SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETN 180

Query: 181 RVALPMSLNLDLTLSRHADDHKL 204
           RVALPMSLNLDLTLSRHADDHKL
Sbjct: 181 RVALPMSLNLDLTLSRHADDHKL 203

BLAST of CSPI02G23910 vs. NCBI nr
Match: XP_016899977.1 (PREDICTED: probable WRKY transcription factor 40 [Cucumis melo])

HSP 1 Score: 306.2 bits (783), Expect = 2.1e-79
Identity = 160/175 (91.43%), Postives = 166/175 (94.86%), Query Frame = 0

Query: 25  MLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSPPALALALETSSTTQAYART 84
           MLKVVSIKNLVSQVDVCSRD  HDQNDEGSRSERA+L+VSPP     LETSSTTQAYA T
Sbjct: 1   MLKVVSIKNLVSQVDVCSRD--HDQNDEGSRSERADLRVSPP----TLETSSTTQAYATT 60

Query: 85  TFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTY 144
           +FKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKK+VQRSLENKSMVIVTY
Sbjct: 61  SFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKEVQRSLENKSMVIVTY 120

Query: 145 DGHHNHNHNHENASPPPLSSSQRGSSSSPPLPVETNRVALPMSLNLDLTLSRHAD 200
           DGHHNHNHNHENASPPPLS+SQRG SSSPPLPVE+NRVALPMSLNLDLTLSRHAD
Sbjct: 121 DGHHNHNHNHENASPPPLSASQRG-SSSPPLPVESNRVALPMSLNLDLTLSRHAD 168

BLAST of CSPI02G23910 vs. NCBI nr
Match: KAA0064889.1 (putative WRKY transcription factor 40 [Cucumis melo var. makuwa])

HSP 1 Score: 303.9 bits (777), Expect = 1.0e-78
Identity = 162/177 (91.53%), Postives = 166/177 (93.79%), Query Frame = 0

Query: 25  MLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSPPALALALETSSTTQAYART 84
           MLKVVSIKNLVSQVDVCSRD  HDQNDEGSRSERA+LKVSPP     LETSSTTQAYA T
Sbjct: 1   MLKVVSIKNLVSQVDVCSRD--HDQNDEGSRSERADLKVSPP----TLETSSTTQAYATT 60

Query: 85  TFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTY 144
           +FKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTY
Sbjct: 61  SFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTY 120

Query: 145 DGH--HNHNHNHENASPPPLSSSQRGSSSSPPLPVETNRVALPMSLNLDLTLSRHAD 200
           DGH  HNHNHNHENASPPPLS+SQRG SSSPPLPVE+NRVALPMSLNLDLTLSRHAD
Sbjct: 121 DGHHNHNHNHNHENASPPPLSASQRG-SSSPPLPVESNRVALPMSLNLDLTLSRHAD 170

BLAST of CSPI02G23910 vs. NCBI nr
Match: XP_038886179.1 (probable WRKY transcription factor 40 [Benincasa hispida])

HSP 1 Score: 266.9 bits (681), Expect = 1.4e-67
Identity = 151/213 (70.89%), Postives = 165/213 (77.46%), Query Frame = 0

Query: 1   MDSKVECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERAN 60
           MDSKVECLQTELE+LRKENEALK ML+VVSIKNLVSQ DVC RD     N   +   RA+
Sbjct: 1   MDSKVECLQTELERLRKENEALKFMLRVVSIKNLVSQTDVCLRDQSDGLNQSSNTRARAD 60

Query: 61  LKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS 120
           L+V    LALAL  SSTTQAY RT+FKDQALMVKDGY+WRKYGQKITKDNQSPRAYFKCS
Sbjct: 61  LEV---PLALALGPSSTTQAYVRTSFKDQALMVKDGYRWRKYGQKITKDNQSPRAYFKCS 120

Query: 121 SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSS--------- 180
           SPGCPVKKKVQRSLE+KSMVIVTYDGH    HNH+N SP PLS+S+R SSS         
Sbjct: 121 SPGCPVKKKVQRSLEDKSMVIVTYDGH----HNHQNGSPSPLSTSRRRSSSLSSPAAVRR 180

Query: 181 --------SPPLPVETNRVALPMSLNLDLTLSR 197
                   SPPL VE+NRV  P+SL+LDLTLSR
Sbjct: 181 LPLPLPPLSPPLWVESNRVVPPVSLDLDLTLSR 206

BLAST of CSPI02G23910 vs. NCBI nr
Match: XP_022132094.1 (WRKY transcription factor WRKY76-like [Momordica charantia])

HSP 1 Score: 164.5 bits (415), Expect = 9.8e-37
Identity = 111/205 (54.15%), Postives = 128/205 (62.44%), Query Frame = 0

Query: 4   KVECLQTELEKLRKENEALKLMLKVVSIKNLV--------SQVDVCSRDHRHDQNDEGSR 63
           KVECLQ ELE+LRK NEAL   L V+S+KNL+        SQV V       D N   ++
Sbjct: 10  KVECLQKELERLRKANEALNFALGVMSMKNLIISRETNTNSQVGVFLEG--SDLNCNSNK 69

Query: 64  SERANLKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRA 123
             RA+L+         L   STTQ Y RT FKDQALM KDGY+WRKYGQKITKDNQ PRA
Sbjct: 70  RARADLQA-----RAGLGAPSTTQTYVRTGFKDQALMAKDGYRWRKYGQKITKDNQYPRA 129

Query: 124 YFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPP- 183
           YF CSS GCPVKKKVQRSLE+KSMV+V+Y       HNHE  SP   S+    S SSP  
Sbjct: 130 YFNCSSRGCPVKKKVQRSLEDKSMVMVSY----QDQHNHECDSPSHGSTRPFLSRSSPVV 189

Query: 184 ---LPVETNRVALPM-SLNLDLTLS 196
              LP       +P+ SL+LDLTL+
Sbjct: 190 VHCLPSTPCNATVPLVSLDLDLTLA 203

BLAST of CSPI02G23910 vs. TAIR 10
Match: AT2G25000.1 (WRKY DNA-binding protein 60 )

HSP 1 Score: 106.7 bits (265), Expect = 2.3e-23
Identity = 72/174 (41.38%), Postives = 101/174 (58.05%), Query Frame = 0

Query: 2   DSKVECLQTELEKLRKENEALKLML-----KVVSIKNLVSQVDV-CSRDHRHDQNDE--G 61
           D K   LQ E+ ++  EN+ L  ML     K  ++ NL+ ++    S +  + QN +  G
Sbjct: 39  DEKRNMLQDEINRVNSENKKLTEMLARVCEKYYALNNLMEELQSRKSPESVNFQNKQLTG 98

Query: 62  SRSERANLKVSPPALALAL--------ETSSTTQAYARTTFKDQALMVKDGYKWRKYGQK 121
            R +  +  VS P + L+L        + ++ + AY      D +L VKDGY+WRKYGQK
Sbjct: 99  KRKQELDEFVSSP-IGLSLGPIENITNDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQK 158

Query: 122 ITKDNQSPRAYFKCS-SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENAS 159
           IT+DN SPRAYF+CS SP C VKKKVQRS E+ S ++ TY+G HNH   H + S
Sbjct: 159 ITRDNPSPRAYFRCSFSPSCLVKKKVQRSAEDPSFLVATYEGTHNHTGPHASVS 211

BLAST of CSPI02G23910 vs. TAIR 10
Match: AT1G80840.1 (WRKY DNA-binding protein 40 )

HSP 1 Score: 102.4 bits (254), Expect = 4.3e-22
Identity = 55/128 (42.97%), Postives = 83/128 (64.84%), Query Frame = 0

Query: 78  TQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGCPVKKKVQRSLEN 137
           ++ Y +T   D  L+VKDGY+WRKYGQK+T+DN SPRAYFKC+ +P C VKKKVQRS+E+
Sbjct: 130 SRVYYKTEASDTTLVVKDGYQWRKYGQKVTRDNPSPRAYFKCACAPSCSVKKKVQRSVED 189

Query: 138 KSMVIVTYDGHHNH--------------NHNHENASPPPLSSSQRGSSSSPPLPV---ET 188
           +S+++ TY+G HNH              + +H  ++  P+++++R S + P   V   E+
Sbjct: 190 QSVLVATYEGEHNHPMPSQIDSNNGLNRHISHGGSASTPVAANRRSSLTVPVTTVDMIES 249

BLAST of CSPI02G23910 vs. TAIR 10
Match: AT4G31800.1 (WRKY DNA-binding protein 18 )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 60/167 (35.93%), Postives = 97/167 (58.08%), Query Frame = 0

Query: 6   ECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSP 65
           E  QT++   +++ +  + +   + + +  ++    + DH H       +++  + K   
Sbjct: 91  EIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTENSSSNEDHHHHHQQHEQKNQLLSCK--- 150

Query: 66  PALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGC 125
             +  +   +  +  Y  T   D +L VKDG++WRKYGQK+T+DN SPRAYF+CS +P C
Sbjct: 151 RPVTDSFNKAKVSTVYVPTETSDTSLTVKDGFQWRKYGQKVTRDNPSPRAYFRCSFAPSC 210

Query: 126 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSS 172
           PVKKKVQRS E+ S+++ TY+G   HNH   NAS    ++SQ GSS+
Sbjct: 211 PVKKKVQRSAEDPSLLVATYEG--THNHLGPNASEGD-ATSQGGSST 251

BLAST of CSPI02G23910 vs. TAIR 10
Match: AT4G31800.2 (WRKY DNA-binding protein 18 )

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-20
Identity = 60/167 (35.93%), Postives = 97/167 (58.08%), Query Frame = 0

Query: 6   ECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSP 65
           E  QT++   +++ +  + +   + + +  ++    + DH H       +++  + K   
Sbjct: 90  EIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTENSSSNEDHHHHHQQHEQKNQLLSCK--- 149

Query: 66  PALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGC 125
             +  +   +  +  Y  T   D +L VKDG++WRKYGQK+T+DN SPRAYF+CS +P C
Sbjct: 150 RPVTDSFNKAKVSTVYVPTETSDTSLTVKDGFQWRKYGQKVTRDNPSPRAYFRCSFAPSC 209

Query: 126 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSS 172
           PVKKKVQRS E+ S+++ TY+G   HNH   NAS    ++SQ GSS+
Sbjct: 210 PVKKKVQRSAEDPSLLVATYEG--THNHLGPNASEGD-ATSQGGSST 250

BLAST of CSPI02G23910 vs. TAIR 10
Match: AT1G13960.1 (WRKY DNA-binding protein 4 )

HSP 1 Score: 85.9 bits (211), Expect = 4.1e-17
Identity = 43/126 (34.13%), Postives = 74/126 (58.73%), Query Frame = 0

Query: 48  DQNDEGSRSERANLKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKIT 107
           D+N+   +     +++S PA A +  T +  +   +TT   +  ++ DGY+WRKYGQK+ 
Sbjct: 365 DENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQTT--SEVDLLDDGYRWRKYGQKVV 424

Query: 108 KDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQR 167
           K N  PR+Y+KC++PGC V+K V+R+  +   V+ TY+G HNH+     +S    +++Q 
Sbjct: 425 KGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDLPAAKSSSHAAAAAQL 484

Query: 168 GSSSSP 174
              + P
Sbjct: 485 RPDNRP 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6IEL01.9e-2245.67WRKY transcription factor WRKY71 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY7... [more]
Q6QHD11.9e-2245.67WRKY transcription factor WRKY71 OS=Oryza sativa subsp. japonica OX=39947 GN=WRK... [more]
Q9SK333.2e-2241.38Probable WRKY transcription factor 60 OS=Arabidopsis thaliana OX=3702 GN=WRKY60 ... [more]
Q6EPZ24.2e-2250.47WRKY transcription factor WRKY76 OS=Oryza sativa subsp. japonica OX=39947 GN=WRK... [more]
Q6IEK59.3e-2250.47WRKY transcription factor WRKY76 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY7... [more]
Match NameE-valueIdentityDescription
A0A0A0LMJ11.5e-107100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G382660 PE=4 SV=1[more]
A0A1S4DVG51.0e-7991.43probable WRKY transcription factor 40 OS=Cucumis melo OX=3656 GN=LOC103488348 PE... [more]
A0A5A7VHE45.0e-7991.53Putative WRKY transcription factor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1BS374.8e-3754.15WRKY transcription factor WRKY76-like OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6P4A7226.0e-3244.72probable WRKY transcription factor 40 OS=Ziziphus jujuba OX=326968 GN=LOC1074197... [more]
Match NameE-valueIdentityDescription
XP_004138843.13.1e-107100.00probable WRKY transcription factor 60 [Cucumis sativus] >KGN62993.1 hypothetical... [more]
XP_016899977.12.1e-7991.43PREDICTED: probable WRKY transcription factor 40 [Cucumis melo][more]
KAA0064889.11.0e-7891.53putative WRKY transcription factor 40 [Cucumis melo var. makuwa][more]
XP_038886179.11.4e-6770.89probable WRKY transcription factor 40 [Benincasa hispida][more]
XP_022132094.19.8e-3754.15WRKY transcription factor WRKY76-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G25000.12.3e-2341.38WRKY DNA-binding protein 60 [more]
AT1G80840.14.3e-2242.97WRKY DNA-binding protein 40 [more]
AT4G31800.11.1e-2035.93WRKY DNA-binding protein 18 [more]
AT4G31800.21.1e-2035.93WRKY DNA-binding protein 18 [more]
AT1G13960.14.1e-1734.13WRKY DNA-binding protein 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 5..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 146..181
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 155..177
NoneNo IPR availablePANTHERPTHR31429:SF3WRKY TRANSCRIPTION FACTOR 40-RELATEDcoord: 4..177
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 93..152
e-value: 1.3E-31
score: 121.0
IPR003657WRKY domainPFAMPF03106WRKYcoord: 94..151
e-value: 2.6E-23
score: 82.0
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 88..153
score: 25.212465
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 77..153
e-value: 1.6E-27
score: 97.1
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 89..152
IPR044810WRKY transcription factor, plantPANTHERPTHR31429WRKY TRANSCRIPTION FACTOR 36-RELATEDcoord: 4..177
IPR014760Serum albumin, N-terminalPROSITEPS51438ALBUMIN_2coord: 1..17
score: 8.658998

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G23910.1CSPI02G23910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005615 extracellular space
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding