CSPI02G19550 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G19550
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.5-like
LocationChr2: 17689949 .. 17692609 (+)
RNA-Seq ExpressionCSPI02G19550
SyntenyCSPI02G19550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTTGTGTCGGTGGCGTTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCAGTGACCACTCTCACTAACTTTATGGGAACAACGTTTCTTCTTACACTACTCGGGGGTTTCATTTCCGAAACTTATCTCTCAAGATTCAAGACTTGCCTTCTCTTTGCTTTCTTTGAGCTCATGGTCAACTCTCTTTCTCTCTCTCTTTCTCTTCCCTTCATTTCCATTTTTGTTTACATTAGACCCAATGTCCGTTTGAATTTGACAGACCATGTTGTTCTATTACATATTCCATCCTCAAACTAAAATAGGATATATTTTCTATATTATCGTCGTGGGTAAACGGATGCAATTTTTCTTTAAATAGAAAGATTTTTACTATCAAGATTGATTTTAGTCTTCTAGAAAATTATAATATATAGTTGAATAATGTTTAAGAGACATTGTTTTACTTTGTTGCATTAGTGGGAGGGATCCTCTTTGCATTCCATATAAATAAATAAAACAATAAAAATAAAATATAACTACCATGTATCAAAATATCCGCCAACGTGAGAGTGTGAATTTTGGTTGAATAAGAATCATTTTTTTTCATCCATGCATCGTGAAATCACCTCATTAAAAAAATATATTTTACAATGATGTTGAAAATGAGATGAATGCATATAAACTACTCATTTTTATATCAATTGAGCTATGTTTAGGTGAACTGATATTATTTAGTAATAGGAAGGTAAAAGTGTTTTAGATACAAATTATGATGACTGAGAGTAAAAATTTGTGCCCAAATGAGCTTCACTAGCGATATTAACATGGTTTTCGACCTCATCCCCACCCACTGTTTTGTTGAAGGATAATAACAATGACTAAAATAGTTATTATGTGGACTATTGTGCAGGGATACTGTCTGCTAACCGTGCAGGCACATTTCCCGCACCTAAGGCCGGCACCTTGCAAAGAGGCACCAGCAGGGAGCGAGTGCGAGGCGGCAGGGAGCAGTCAGGAGGCGTTTCTTTACACGGGATTATACCTGATAGCCCTGGGAACGAGTGGCGTGAAAGCAGCGCTGCCAGCATTGGGGGCAGATCAATTCGATGACAAAGATCCAAGCGAGGCGGGGAAAGTTTCGAGCTTTTTCAACTGGTTCCTGTTCAGCCTAACAATAGGGTCAATTGTGGGATTGACATTGATAGTGTGGATAAACACAGAGGTAGGTTGGGACTGGGCGTTTGTGGTTTGCAGCATTTCAGTTCTTGCCGCCATTTTTGTTGTGTTTTTAGGCAAGTCCTTTTATCGCCACAATGTGCCTCAAGGAAGCCCCATTCTTAGGTTTCTACAGGTTCATATTTCTTTTCCTGATATGCAGTCTCTTTGAACTCATCAATCATGATGCAATGAATGGTTGTTTGATCCTTTTTATTCTCATGTATAGGTCTTTGTTGCTTCAATCCGAAACAGGAAGCTTCCACTTCCAGAGAATGCTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAATACTCGAGAAAACTGATCAATTCAGGTATTATACTTTTTAATGATCTTCATATAAAGAACTAAGCTTTGCAATACAATTGGTTGGTTGAAGCCATGGAATTTTGGAACTTTGTAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAATTGCATCAATATCAACAAATCAGCAAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAGGAAACAAAGATCCTAATTAGAATGCTCCCAATTATACTCAGCACCATCTTCATGGCCACCTGCATGGCTCAACTTCAAACATTCTCCATTCAACAAAGTATAACCATGGATTCCCAGTTCCTGGGATTCAAAATTCCGGGCCCCTCCATCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTGTTTTCGTTCCTCTCGCTCGTAAAATTACCGGGATTCCAACCGGAATTCGTCACCTCCAGAGAATTGGCATTGGACTCGTACTCACTGCCGCATCGATGGCCATTGCTGGGTTTGTTGAGACTCGACGCAAAAACGTTGCAATCAAACACAATATGGTGGATAGTACAGAGCCACTGCCGATCAGCGTTTTCTGGTTGGGATTTCAGTTCTGCGTATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAGAGCTCAGCAGGGATGAAATCGTTGAGCACAGCTATTGCTTGGTGTGCGATTGCATTCGGGTATTTCACGAGTACGGTGGTGGTTACAGTGGTGAACAAGGCGAGTGGAGGATGGCTGGCAAGCAACAATCTCAACAAAGACAAGTTGGACTATTTCTACTGGTTATTGTCAGTGTTGAGTGTGTTGAATTTTGGGTTTTATTTGGTTTGTGCTTCCTGGTATAGGTATAAGAATGTGGAGATCCATCAAAACGATGCTCTGGAAGAAAAGGTTGACATGGCAAGGGAGGCATAGGTACATGTTTGGTTGCTGTTAAGGTCTAAATATAATATTTCCTGGTAAATTGTTTTCATCAGTCCTTGTAATATGCTCCTTGTCATGGTGTGTTCGTATATATTCTTTATAAAAAAAGAATACTTCACAGC

mRNA sequence

ATGGCATTTGTGTCGGTGGCGTTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCAGTGACCACTCTCACTAACTTTATGGGAACAACGTTTCTTCTTACACTACTCGGGGGTTTCATTTCCGAAACTTATCTCTCAAGATTCAAGACTTGCCTTCTCTTTGCTTTCTTTGAGCTCATGGGATACTGTCTGCTAACCGTGCAGGCACATTTCCCGCACCTAAGGCCGGCACCTTGCAAAGAGGCACCAGCAGGGAGCGAGTGCGAGGCGGCAGGGAGCAGTCAGGAGGCGTTTCTTTACACGGGATTATACCTGATAGCCCTGGGAACGAGTGGCGTGAAAGCAGCGCTGCCAGCATTGGGGGCAGATCAATTCGATGACAAAGATCCAAGCGAGGCGGGGAAAGTTTCGAGCTTTTTCAACTGGTTCCTGTTCAGCCTAACAATAGGGTCAATTGTGGGATTGACATTGATAGTGTGGATAAACACAGAGGTAGGTTGGGACTGGGCGTTTGTGGTTTGCAGCATTTCAGTTCTTGCCGCCATTTTTGTTGTGTTTTTAGGCAAGTCCTTTTATCGCCACAATGTGCCTCAAGGAAGCCCCATTCTTAGGTTTCTACAGGTCTTTGTTGCTTCAATCCGAAACAGGAAGCTTCCACTTCCAGAGAATGCTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAATACTCGAGAAAACTGATCAATTCAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAATTGCATCAATATCAACAAATCAGCAAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAGGAAACAAAGATCCTAATTAGAATGCTCCCAATTATACTCAGCACCATCTTCATGGCCACCTGCATGGCTCAACTTCAAACATTCTCCATTCAACAAAGTATAACCATGGATTCCCAGTTCCTGGGATTCAAAATTCCGGGCCCCTCCATCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTGTTTTCGTTCCTCTCGCTCGTAAAATTACCGGGATTCCAACCGGAATTCGTCACCTCCAGAGAATTGGCATTGGACTCGTACTCACTGCCGCATCGATGGCCATTGCTGGGTTTGTTGAGACTCGACGCAAAAACGTTGCAATCAAACACAATATGGTGGATAGTACAGAGCCACTGCCGATCAGCGTTTTCTGGTTGGGATTTCAGTTCTGCGTATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAGAGCTCAGCAGGGATGAAATCGTTGAGCACAGCTATTGCTTGGTGTGCGATTGCATTCGGGTATTTCACGAGTACGGTGGTGGTTACAGTGGTGAACAAGGCGAGTGGAGGATGGCTGGCAAGCAACAATCTCAACAAAGACAAGTTGGACTATTTCTACTGGTTATTGTCAGTGTTGAGTGTGTTGAATTTTGGGTTTTATTTGGTTTGTGCTTCCTGGTATAGGTATAAGAATGTGGAGATCCATCAAAACGATGCTCTGGAAGAAAAGGTTGACATGGCAAGGGAGGCATAGGTACATGTTTGGTTGCTGTTAAGGTCTAAATATAATATTTCCTGGTAAATTGTTTTCATCAGTCCTTGTAATATGCTCCTTGTCATGGTGTGTTCGTATATATTCTTTATAAAAAAAGAATACTTCACAGC

Coding sequence (CDS)

ATGGCATTTGTGTCGGTGGCGTTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCAGTGACCACTCTCACTAACTTTATGGGAACAACGTTTCTTCTTACACTACTCGGGGGTTTCATTTCCGAAACTTATCTCTCAAGATTCAAGACTTGCCTTCTCTTTGCTTTCTTTGAGCTCATGGGATACTGTCTGCTAACCGTGCAGGCACATTTCCCGCACCTAAGGCCGGCACCTTGCAAAGAGGCACCAGCAGGGAGCGAGTGCGAGGCGGCAGGGAGCAGTCAGGAGGCGTTTCTTTACACGGGATTATACCTGATAGCCCTGGGAACGAGTGGCGTGAAAGCAGCGCTGCCAGCATTGGGGGCAGATCAATTCGATGACAAAGATCCAAGCGAGGCGGGGAAAGTTTCGAGCTTTTTCAACTGGTTCCTGTTCAGCCTAACAATAGGGTCAATTGTGGGATTGACATTGATAGTGTGGATAAACACAGAGGTAGGTTGGGACTGGGCGTTTGTGGTTTGCAGCATTTCAGTTCTTGCCGCCATTTTTGTTGTGTTTTTAGGCAAGTCCTTTTATCGCCACAATGTGCCTCAAGGAAGCCCCATTCTTAGGTTTCTACAGGTCTTTGTTGCTTCAATCCGAAACAGGAAGCTTCCACTTCCAGAGAATGCTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAATACTCGAGAAAACTGATCAATTCAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAATTGCATCAATATCAACAAATCAGCAAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAGGAAACAAAGATCCTAATTAGAATGCTCCCAATTATACTCAGCACCATCTTCATGGCCACCTGCATGGCTCAACTTCAAACATTCTCCATTCAACAAAGTATAACCATGGATTCCCAGTTCCTGGGATTCAAAATTCCGGGCCCCTCCATCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTGTTTTCGTTCCTCTCGCTCGTAAAATTACCGGGATTCCAACCGGAATTCGTCACCTCCAGAGAATTGGCATTGGACTCGTACTCACTGCCGCATCGATGGCCATTGCTGGGTTTGTTGAGACTCGACGCAAAAACGTTGCAATCAAACACAATATGGTGGATAGTACAGAGCCACTGCCGATCAGCGTTTTCTGGTTGGGATTTCAGTTCTGCGTATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAGAGCTCAGCAGGGATGAAATCGTTGAGCACAGCTATTGCTTGGTGTGCGATTGCATTCGGGTATTTCACGAGTACGGTGGTGGTTACAGTGGTGAACAAGGCGAGTGGAGGATGGCTGGCAAGCAACAATCTCAACAAAGACAAGTTGGACTATTTCTACTGGTTATTGTCAGTGTTGAGTGTGTTGAATTTTGGGTTTTATTTGGTTTGTGCTTCCTGGTATAGGTATAAGAATGTGGAGATCCATCAAAACGATGCTCTGGAAGAAAAGGTTGACATGGCAAGGGAGGCATAG

Protein sequence

MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLFAFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKEAAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMAREA*
Homology
BLAST of CSPI02G19550 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 5.7e-111
Identity = 219/529 (41.40%), Postives = 328/529 (62.00%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL LLGGF+++ + S F   L+ 
Sbjct: 46  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 105

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E +G  LLT+QA  P L P PCK + A   CE  G S+ AFL+ GLYL++LG  G+K
Sbjct: 106 ASIEFLGLILLTIQARRPSLMPPPCKSS-AALRCEVVGGSKAAFLFVGLYLVSLGIGGIK 165

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V +T +VWI    GW+W F V 
Sbjct: 166 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 225

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFV-ASIRNRKLPLPENANELHEIR-D 240
           +IS+  +I V  LG  FY++ +P+GSP+    +V + ASI +       N     E++ +
Sbjct: 226 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 285

Query: 241 KEAAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          I       W  CTV QVE+ KI+++MLP
Sbjct: 286 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 345

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
           I   TI +  C+AQL T+S+ Q+ TM+ + + F +P  S+PV P++FM    P Y+ + +
Sbjct: 346 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 405

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A  VE +RK VA +  ++DS E LPI+ 
Sbjct: 406 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 465

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W ++A GY+ S+V+V +VN
Sbjct: 466 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 525

Query: 481 KAS-----GGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRY 523
           + +       WL    LN+++LD FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 526 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of CSPI02G19550 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 2.4e-109
Identity = 229/549 (41.71%), Postives = 331/549 (60.29%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MA  +V  +L+TY    M+F L+KS   +TNF+GT FLL+LLGGF+S++YL  F+T L+F
Sbjct: 57  MAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVF 116

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
              E+ G+ LL+VQAH P LRP  C        C  A   + A LYT L L+ALG+  +K
Sbjct: 117 GVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLK 176

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
             + + GA+QF  KD     K+SSFFN   F+ ++G ++ LTL+VW+ T  G D  F V 
Sbjct: 177 PNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVS 236

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           +  + A +  +  G SFYR+  P GS      QVFVA+I  RK   P N N +H+     
Sbjct: 237 AAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQ--PST 296

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
             +  + L  +++FRFLD+A       I +     + PWRLCT+ QV + KIL+ ++PI 
Sbjct: 297 DLVRVKPLLHSNKFRFLDKAC------IKTQGKAMESPWRLCTIEQVHQVKILLSVIPIF 356

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERVFVP 360
             TI   T +AQLQTFS+QQ  +M++     F+IP  S+  IP + + FF+P YE  FVP
Sbjct: 357 ACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVP 416

Query: 361 LARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVF 420
           LARK+TG  +GI  LQRIG GL L   SM  A  VE +R+   ++ N++       +S+F
Sbjct: 417 LARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM-------LSIF 476

Query: 421 WLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNK 480
           W+  QF +FG+ ++FT VGL+EFFY +SS  M+S  TA+ +C+ +FG++ S+V+V+ VN+
Sbjct: 477 WIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNR 536

Query: 481 ASG--------GWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWY------RYKNV 535
            +         GWL  N+LNKD+LD+FYWLL+ LS +NF  YL  + WY       + + 
Sbjct: 537 VTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSATHHSA 587

BLAST of CSPI02G19550 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 5.4e-109
Identity = 214/541 (39.56%), Postives = 330/541 (61.00%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +A+++ A +LV Y   YM+ S +KS   +TNFMGT FLL LLGGF+S+ + S F+  L+ 
Sbjct: 44  LAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLIS 103

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E +G  +LT+QA  P L P  C ++P    CE    S+ A L+ GLYL+ALG  G+K
Sbjct: 104 ASIEFLGLIILTIQARTPSLMPPSC-DSPT---CEEVSGSKAAMLFVGLYLVALGVGGIK 163

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +L + GA+QFD+  P    + S+FFN+F+F L  G++V +T +VW+    GW+W F V 
Sbjct: 164 GSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVS 223

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFV-ASIRNRKLPLPENA--------- 240
           +I++  +I +   G  FYR+ +P GSP+   L+V + AS++        NA         
Sbjct: 224 TIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPS 283

Query: 241 NELHEIRDKEAAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRL--CTVTQVE 300
           N       KE     E+ +   +     RA +  +  + + + +++   RL  CTV QVE
Sbjct: 284 NHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVE 343

Query: 301 ETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFF 360
           + KI+++MLPI   TI +  C+AQL TFS+QQ+ +M+++    KIP  S+P+ P++F+  
Sbjct: 344 DVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMI 403

Query: 361 FIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMV 420
             P Y+ + +P ARK T   TG+ HLQRIG+GLVL+  +MA+A  VE +RK VA    ++
Sbjct: 404 LAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLL 463

Query: 421 DSTEPLPISVFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYF 480
           DS E LP++  W+  Q+   G  D+FTL GLLE+F+ E+ + M+SL+T+++W ++A GY+
Sbjct: 464 DSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYY 523

Query: 481 TSTVVVTVVNKASGG-----WLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYK 525
            S+V+V++VN  +G      WL   ++N+ KLDYFYWL+ VLS  NF  YL  A  Y+Y+
Sbjct: 524 LSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 580

BLAST of CSPI02G19550 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 4.0e-104
Identity = 216/527 (40.99%), Postives = 316/527 (59.96%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           M   +V  +L+TY    M+F L+K+   +TNF+GT F+  LLGG++S+ +L  F T ++F
Sbjct: 60  MGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIF 119

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
            F EL G+ LL+VQAH P L+P  C        CE A   +    +  LYL+ALG+  VK
Sbjct: 120 GFVELSGFILLSVQAHLPQLKPPKCNPL-IDQTCEEAKGFKAMIFFMALYLVALGSGCVK 179

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
             + A GADQF    P ++ ++SS+FN   F+ ++G ++ LTL+VW+ T  G D  F V 
Sbjct: 180 PNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVS 239

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELH-EIRDK 240
           + ++   I  +  G  ++R+  P+ S       V VA+I  RKL  P +   LH +    
Sbjct: 240 AAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVA 299

Query: 241 EAAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPI 300
              +P   L  T +FRFLD+A I   DT       ++ PWRLCTVTQVE+ K LI ++PI
Sbjct: 300 NDVVPSSTLPHTPRFRFLDKACIKIQDT-----NTKESPWRLCTVTQVEQVKTLISLVPI 359

Query: 301 ILSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
             STI   T +AQLQTFS+QQ  +M+++    F IP  S+  IP + + F +P Y+   V
Sbjct: 360 FASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLV 419

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+TG  +GI  L RIGIGL L+  SM  A  +E +R++ ++    +       +S+
Sbjct: 420 PFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-------LSI 479

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
           FW+  QF +FG+ ++FT VGL+EFFY +S+ GM+S   A+ +C+ +FG++ S+V+V+VVN
Sbjct: 480 FWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVN 539

Query: 481 KA------SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASW 520
           K       S GWL  N+LNKD+LD FYWLL+VLS+LNF  YL  + W
Sbjct: 540 KITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of CSPI02G19550 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.9e-98
Identity = 215/540 (39.81%), Postives = 320/540 (59.26%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +A V    ++++Y +  ++  LTK+  TLTNF GT+ L  LLG FI++++  RF T    
Sbjct: 42  LAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFA 101

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSE-CEAAGSSQEAFLYTGLYLIALGTSGV 120
           +    +G  LLT+ A  P LRP PCK    G E C  A ++Q + LY  L L ALG+ G+
Sbjct: 102 SIIYQIGMTLLTISAIIPTLRPPPCK----GEEVCVVADTAQLSILYVALLLGALGSGGI 161

Query: 121 KAALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVV 180
           +  + A GADQFD+ DP++  K  ++FNW+ F +    ++ +T++VWI   VGW     +
Sbjct: 162 RPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGI 221

Query: 181 CSISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDK 240
            ++++  ++     G   YRH VP GSP  R +QV VA+ R RKL +  + + L+   + 
Sbjct: 222 PTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEI 281

Query: 241 EAAIPY-EILEKTDQFRFLDRAAIIRNDTIASISTNQ-QGPWRLCTVTQVEETKILIRML 300
           +A I     L  T    FLD+AAI+  +   ++   Q    WRL TV +VEE K +IRM 
Sbjct: 282 DAPISLGGKLTHTKHMSFLDKAAIVTEED--NLKPGQIPNHWRLSTVHRVEELKSVIRMG 341

Query: 301 PIILSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERV 360
           PI  S I + T  AQ  TFS+QQ+ TM+      F+IP  S+ V   + M   I FY+RV
Sbjct: 342 PIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRV 401

Query: 361 FVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPL-P 420
           FV +ARK TG+  GI  L R+GIG V++  +  +AGFVE +RK+VAI+H ++D    + P
Sbjct: 402 FVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVP 461

Query: 421 ISVFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVT 480
           IS  WL  Q+ + G+ + F  +G LEFFY ++   M+S +TA+ W AI+ G + ST++VT
Sbjct: 462 ISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVT 521

Query: 481 VVNKAS-----GGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQN 531
           +V+K S       WL  NNLN+ +L+YFYWL++VL  +N  +YL CA  Y YK V++H +
Sbjct: 522 LVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWCAKIYTYKPVQVHHS 575

BLAST of CSPI02G19550 vs. ExPASy TrEMBL
Match: A0A0A0LLC0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360700 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.7e-270
Identity = 476/479 (99.37%), Postives = 477/479 (99.58%), Query Frame = 0

Query: 65  LMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALP 124
           + GYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALP
Sbjct: 11  VQGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALP 70

Query: 125 ALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISV 184
           ALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISV
Sbjct: 71  ALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISV 130

Query: 185 LAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKEAAIP 244
           LAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLP NANELHEIRDKEAAIP
Sbjct: 131 LAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKEAAIP 190

Query: 245 YEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTI 304
           YEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTI
Sbjct: 191 YEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTI 250

Query: 305 FMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKI 364
           FMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKI
Sbjct: 251 FMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKI 310

Query: 365 TGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQ 424
           TGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQ
Sbjct: 311 TGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQ 370

Query: 425 FCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGW 484
           FCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGW
Sbjct: 371 FCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGW 430

Query: 485 LASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMAREA 544
           LASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMAREA
Sbjct: 431 LASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMAREA 489

BLAST of CSPI02G19550 vs. ExPASy TrEMBL
Match: A0A6J1CR11 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 9.1e-261
Identity = 467/543 (86.00%), Postives = 498/543 (91.71%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTLLGGFIS+TYLSRFKTCL+F
Sbjct: 37  MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 96

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E MGY LLT QAHF  LRPAPCK A A  ECEAA   QEA LY G+YLIALGTSGVK
Sbjct: 97  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 156

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           +ALPALGADQFD  DP+EAGK+SSFFNWFLFSLT GSIVGLTLIVWINT+VGWDWAFVVC
Sbjct: 157 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 216

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           S++V+AAI  V  GK FYR+N P+GSPI R  QVFVASIRNRKLPLP+NA+ELHEIRDKE
Sbjct: 217 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 276

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYEILEKTDQFRFLDRAAIIRN T AS++  QQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 277 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 336

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDS FLGFKIPGPS+PVIPLLFM F IPFYERVFVPL
Sbjct: 337 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 396

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKN+AI+H+MVDS EPLP+SVFW
Sbjct: 397 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 456

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFC+FGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 457 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 516

Query: 481 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 540
           SGGWL SNNLN+DKL+YFYWLLSVLSVLNFGFYL+CASWYRYKNVEI QND  EEK D+A
Sbjct: 517 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKDDIA 576

Query: 541 REA 544
           R A
Sbjct: 577 RGA 578

BLAST of CSPI02G19550 vs. ExPASy TrEMBL
Match: A0A6J1CSN2 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 9.1e-261
Identity = 467/543 (86.00%), Postives = 498/543 (91.71%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTLLGGFIS+TYLSRFKTCL+F
Sbjct: 1   MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 60

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E MGY LLT QAHF  LRPAPCK A A  ECEAA   QEA LY G+YLIALGTSGVK
Sbjct: 61  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 120

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           +ALPALGADQFD  DP+EAGK+SSFFNWFLFSLT GSIVGLTLIVWINT+VGWDWAFVVC
Sbjct: 121 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 180

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           S++V+AAI  V  GK FYR+N P+GSPI R  QVFVASIRNRKLPLP+NA+ELHEIRDKE
Sbjct: 181 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 240

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYEILEKTDQFRFLDRAAIIRN T AS++  QQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 241 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 300

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDS FLGFKIPGPS+PVIPLLFM F IPFYERVFVPL
Sbjct: 301 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 360

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKN+AI+H+MVDS EPLP+SVFW
Sbjct: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 420

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFC+FGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 421 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480

Query: 481 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 540
           SGGWL SNNLN+DKL+YFYWLLSVLSVLNFGFYL+CASWYRYKNVEI QND  EEK D+A
Sbjct: 481 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKDDIA 540

Query: 541 REA 544
           R A
Sbjct: 541 RGA 542

BLAST of CSPI02G19550 vs. ExPASy TrEMBL
Match: A0A6J1GIY5 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 PE=3 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 9.1e-253
Identity = 450/541 (83.18%), Postives = 489/541 (90.39%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFV++ALSL+TYF+GYMNFSLTKS TTLTNFMGTTFLLTL+GGF+S+TYLSRFKTC+LF
Sbjct: 34  MAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLF 93

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A FEL+GYCLLT QAHF  LRP PCK A AGSEC+AA   QEA LY G+YLIALG SGVK
Sbjct: 94  ACFELLGYCLLTAQAHFRELRPEPCKGAAAGSECKAANGGQEAILYVGIYLIALGASGVK 153

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            ALPALGADQF++ DP+EAGK+SSFFNWFLFSLT GS VGLTLIVWINTEVGWDWAFVVC
Sbjct: 154 VALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVC 213

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           + +VLAAI VV  GKSF+R+NVP+GSPILR LQV VASIRNRKLPLP NA+ELHEIRDKE
Sbjct: 214 TAAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNADELHEIRDKE 273

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           A IP EILEKTDQFRFL+RAAIIRNDT        + PWRLCTVTQVEETKILIRMLPII
Sbjct: 274 ADIPIEILEKTDQFRFLERAAIIRNDT--------RTPWRLCTVTQVEETKILIRMLPII 333

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMA+CMAQLQTFSIQQS+TMDS F+GFKIPGPS+PVIPLLFMFFFIPFYERVFVPL
Sbjct: 334 VSTIFMASCMAQLQTFSIQQSLTMDSNFIGFKIPGPSVPVIPLLFMFFFIPFYERVFVPL 393

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIG GL+LTAASMAIAGFVETRRKNVAI+HNMVDS EPLP+SVFW
Sbjct: 394 ARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNMVDSEEPLPMSVFW 453

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFCVFGM DIFT+VGLLEFFYAESSA MKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 454 LSFQFCVFGMADIFTMVGLLEFFYAESSAEMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 513

Query: 481 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 540
           SGGWLASNNLN+D L+YFYWLLSVLSV+NFGFYLVCASWYRYKNVE+ Q    EE  D+ 
Sbjct: 514 SGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVLQKQG-EEGFDIV 565

Query: 541 R 542
           +
Sbjct: 574 Q 565

BLAST of CSPI02G19550 vs. ExPASy TrEMBL
Match: A0A6J1KKU7 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.5e-252
Identity = 452/543 (83.24%), Postives = 490/543 (90.24%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFV++ALSL+TYFSGYMNFSLTKS TT+TNFMGTTFLLTL+GGF+S+TYLSRFKTC+LF
Sbjct: 49  MAFVALALSLMTYFSGYMNFSLTKSATTMTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLF 108

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A FEL+GY LL+ QAHF  LRP PCK A AGSEC+AA   QEA LY G+YLIALG SGVK
Sbjct: 109 ACFELLGYFLLSAQAHFRELRPEPCKGALAGSECKAANGGQEAILYVGIYLIALGASGVK 168

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            ALPALGADQF++ DP+EAGK+SSFFNWFLFSLT GS VGLTLIVWINTEVGWDWAFVVC
Sbjct: 169 VALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVC 228

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           +I+VLAAI VV  GKSF+R+NVP+GSPILR LQV VASI NRKLPLPENA+ELHEIRDKE
Sbjct: 229 TIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIHNRKLPLPENADELHEIRDKE 288

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           A IP EILEKTDQFRFL+RAAIIRNDT        +  WRLCTVTQVEETKILIRMLPII
Sbjct: 289 ADIPIEILEKTDQFRFLERAAIIRNDT--------RTLWRLCTVTQVEETKILIRMLPII 348

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQS+TMDS FLGFKIPGPS+PVIPLLFMFFFIPFYERVFVPL
Sbjct: 349 VSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVIPLLFMFFFIPFYERVFVPL 408

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIG GL+LTAASMAIAGFVETRRKNVAI+HNMVDS EPLP+SVFW
Sbjct: 409 ARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNMVDSEEPLPMSVFW 468

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFCVFGM DIFT+VGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 469 LSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 528

Query: 481 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 540
           SGGWLASNNLN+D L+YFYWLLSVLSV+NFGFYLVCASWYRYKNVE+ Q    EE  D+ 
Sbjct: 529 SGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVLQKQG-EEGFDIV 582

Query: 541 REA 544
           + A
Sbjct: 589 QGA 582

BLAST of CSPI02G19550 vs. NCBI nr
Match: XP_011649593.2 (protein NRT1/ PTR FAMILY 4.5 [Cucumis sativus])

HSP 1 Score: 1057.0 bits (2732), Expect = 5.6e-305
Identity = 542/543 (99.82%), Postives = 542/543 (99.82%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF
Sbjct: 25  MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 84

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK
Sbjct: 85  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 144

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC
Sbjct: 145 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 204

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLP NANELHEIRDKE
Sbjct: 205 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKE 264

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 265 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 324

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL
Sbjct: 325 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 384

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW
Sbjct: 385 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 444

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA
Sbjct: 445 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 504

Query: 481 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 540
           SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA
Sbjct: 505 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 564

Query: 541 REA 544
           REA
Sbjct: 565 REA 567

BLAST of CSPI02G19550 vs. NCBI nr
Match: XP_038885750.1 (protein NRT1/ PTR FAMILY 4.5-like [Benincasa hispida])

HSP 1 Score: 961.8 bits (2485), Expect = 2.4e-276
Identity = 487/541 (90.02%), Postives = 511/541 (94.45%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYL RFKTC+LF
Sbjct: 61  MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLPRFKTCVLF 120

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A FELMGYCLLT QAHFPHLRPAPCK A A SECEAAGS QEA LY GLYLIALGTSGVK
Sbjct: 121 ASFELMGYCLLTAQAHFPHLRPAPCKGAAARSECEAAGSGQEAILYVGLYLIALGTSGVK 180

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           AALPALGADQFDD+DP+EA KVSSFFNWFLFSLTIGSIVG+TLIVWINTEVGWDWAFVVC
Sbjct: 181 AALPALGADQFDDRDPAEAAKVSSFFNWFLFSLTIGSIVGVTLIVWINTEVGWDWAFVVC 240

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           SI++L A+ VVF GKSFYR+NVP+GSPILR LQVFVASIRNRKLPLPEN NELHEIRDKE
Sbjct: 241 SIAILVAVVVVFAGKSFYRNNVPKGSPILRLLQVFVASIRNRKLPLPENVNELHEIRDKE 300

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AAIP+EILEKTDQFRFLDRAAI+RNDT AS+S NQ GPWRLCTVTQVEETKILIRMLPII
Sbjct: 301 AAIPHEILEKTDQFRFLDRAAIVRNDTNASMSANQPGPWRLCTVTQVEETKILIRMLPII 360

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMD QFLGFKIPGPS+PVIPLLFM FFIPFYE VFVPL
Sbjct: 361 VSTIFMATCMAQLQTFSIQQSITMDCQFLGFKIPGPSVPVIPLLFMIFFIPFYEHVFVPL 420

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLV+T AS+AIA  VETRRKN+AIKHNMVDS EPLP++VFW
Sbjct: 421 ARKITGIPTGIRHLQRIGIGLVITTASIAIAAVVETRRKNIAIKHNMVDSEEPLPMTVFW 480

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 481 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 540

Query: 481 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 540
           SGGWLASNNLN+D LDYFYWLLSVLSVLNFGFYLVCASWY YKNV + QNDA+EEKVD+A
Sbjct: 541 SGGWLASNNLNRDNLDYFYWLLSVLSVLNFGFYLVCASWYTYKNVGVLQNDAMEEKVDIA 600

Query: 541 R 542
           R
Sbjct: 601 R 601

BLAST of CSPI02G19550 vs. NCBI nr
Match: KAE8652098.1 (hypothetical protein Csa_022418 [Cucumis sativus])

HSP 1 Score: 934.5 bits (2414), Expect = 4.2e-268
Identity = 477/486 (98.15%), Postives = 477/486 (98.15%), Query Frame = 0

Query: 66  MGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPA 125
           MGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPA
Sbjct: 70  MGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPA 129

Query: 126 LGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISVL 185
           LGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISVL
Sbjct: 130 LGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISVL 189

Query: 186 AAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKEAAIPY 245
           AAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLP NANELHEIRDKEAAIPY
Sbjct: 190 AAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKEAAIPY 249

Query: 246 EILEKTDQF--------RFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRML 305
           EILEKTDQF        RFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRML
Sbjct: 250 EILEKTDQFSHGILELCRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRML 309

Query: 306 PIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVF 365
           PIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVF
Sbjct: 310 PIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVF 369

Query: 366 VPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPIS 425
           VPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPIS
Sbjct: 370 VPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPIS 429

Query: 426 VFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVV 485
           VFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVV
Sbjct: 430 VFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVV 489

Query: 486 NKASGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKV 544
           NKASGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKV
Sbjct: 490 NKASGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKV 549

BLAST of CSPI02G19550 vs. NCBI nr
Match: XP_022144216.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Momordica charantia])

HSP 1 Score: 909.1 bits (2348), Expect = 1.9e-260
Identity = 467/543 (86.00%), Postives = 498/543 (91.71%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTLLGGFIS+TYLSRFKTCL+F
Sbjct: 1   MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 60

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E MGY LLT QAHF  LRPAPCK A A  ECEAA   QEA LY G+YLIALGTSGVK
Sbjct: 61  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 120

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           +ALPALGADQFD  DP+EAGK+SSFFNWFLFSLT GSIVGLTLIVWINT+VGWDWAFVVC
Sbjct: 121 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 180

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           S++V+AAI  V  GK FYR+N P+GSPI R  QVFVASIRNRKLPLP+NA+ELHEIRDKE
Sbjct: 181 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 240

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYEILEKTDQFRFLDRAAIIRN T AS++  QQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 241 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 300

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDS FLGFKIPGPS+PVIPLLFM F IPFYERVFVPL
Sbjct: 301 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 360

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKN+AI+H+MVDS EPLP+SVFW
Sbjct: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 420

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFC+FGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 421 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480

Query: 481 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 540
           SGGWL SNNLN+DKL+YFYWLLSVLSVLNFGFYL+CASWYRYKNVEI QND  EEK D+A
Sbjct: 481 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKDDIA 540

Query: 541 REA 544
           R A
Sbjct: 541 RGA 542

BLAST of CSPI02G19550 vs. NCBI nr
Match: XP_022144215.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 [Momordica charantia])

HSP 1 Score: 909.1 bits (2348), Expect = 1.9e-260
Identity = 467/543 (86.00%), Postives = 498/543 (91.71%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTLLGGFIS+TYLSRFKTCL+F
Sbjct: 37  MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 96

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E MGY LLT QAHF  LRPAPCK A A  ECEAA   QEA LY G+YLIALGTSGVK
Sbjct: 97  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 156

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           +ALPALGADQFD  DP+EAGK+SSFFNWFLFSLT GSIVGLTLIVWINT+VGWDWAFVVC
Sbjct: 157 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 216

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           S++V+AAI  V  GK FYR+N P+GSPI R  QVFVASIRNRKLPLP+NA+ELHEIRDKE
Sbjct: 217 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 276

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYEILEKTDQFRFLDRAAIIRN T AS++  QQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 277 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 336

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDS FLGFKIPGPS+PVIPLLFM F IPFYERVFVPL
Sbjct: 337 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 396

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKN+AI+H+MVDS EPLP+SVFW
Sbjct: 397 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 456

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFC+FGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 457 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 516

Query: 481 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 540
           SGGWL SNNLN+DKL+YFYWLLSVLSVLNFGFYL+CASWYRYKNVEI QND  EEK D+A
Sbjct: 517 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKDDIA 576

Query: 541 REA 544
           R A
Sbjct: 577 RGA 578

BLAST of CSPI02G19550 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 402.9 bits (1034), Expect = 4.1e-112
Identity = 219/529 (41.40%), Postives = 328/529 (62.00%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL LLGGF+++ + S F   L+ 
Sbjct: 42  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 101

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E +G  LLT+QA  P L P PCK + A   CE  G S+ AFL+ GLYL++LG  G+K
Sbjct: 102 ASIEFLGLILLTIQARRPSLMPPPCKSS-AALRCEVVGGSKAAFLFVGLYLVSLGIGGIK 161

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V +T +VWI    GW+W F V 
Sbjct: 162 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 221

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFV-ASIRNRKLPLPENANELHEIR-D 240
           +IS+  +I V  LG  FY++ +P+GSP+    +V + ASI +       N     E++ +
Sbjct: 222 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 281

Query: 241 KEAAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          I       W  CTV QVE+ KI+++MLP
Sbjct: 282 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 341

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
           I   TI +  C+AQL T+S+ Q+ TM+ + + F +P  S+PV P++FM    P Y+ + +
Sbjct: 342 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 401

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A  VE +RK VA +  ++DS E LPI+ 
Sbjct: 402 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 461

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W ++A GY+ S+V+V +VN
Sbjct: 462 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 521

Query: 481 KAS-----GGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRY 523
           + +       WL    LN+++LD FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 522 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of CSPI02G19550 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 402.9 bits (1034), Expect = 4.1e-112
Identity = 219/529 (41.40%), Postives = 328/529 (62.00%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL LLGGF+++ + S F   L+ 
Sbjct: 46  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 105

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E +G  LLT+QA  P L P PCK + A   CE  G S+ AFL+ GLYL++LG  G+K
Sbjct: 106 ASIEFLGLILLTIQARRPSLMPPPCKSS-AALRCEVVGGSKAAFLFVGLYLVSLGIGGIK 165

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V +T +VWI    GW+W F V 
Sbjct: 166 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 225

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFV-ASIRNRKLPLPENANELHEIR-D 240
           +IS+  +I V  LG  FY++ +P+GSP+    +V + ASI +       N     E++ +
Sbjct: 226 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 285

Query: 241 KEAAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          I       W  CTV QVE+ KI+++MLP
Sbjct: 286 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 345

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
           I   TI +  C+AQL T+S+ Q+ TM+ + + F +P  S+PV P++FM    P Y+ + +
Sbjct: 346 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 405

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A  VE +RK VA +  ++DS E LPI+ 
Sbjct: 406 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 465

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W ++A GY+ S+V+V +VN
Sbjct: 466 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 525

Query: 481 KAS-----GGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRY 523
           + +       WL    LN+++LD FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 526 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of CSPI02G19550 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 397.5 bits (1020), Expect = 1.7e-110
Identity = 229/549 (41.71%), Postives = 331/549 (60.29%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MA  +V  +L+TY    M+F L+KS   +TNF+GT FLL+LLGGF+S++YL  F+T L+F
Sbjct: 57  MAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVF 116

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
              E+ G+ LL+VQAH P LRP  C        C  A   + A LYT L L+ALG+  +K
Sbjct: 117 GVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLK 176

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
             + + GA+QF  KD     K+SSFFN   F+ ++G ++ LTL+VW+ T  G D  F V 
Sbjct: 177 PNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVS 236

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELHEIRDKE 240
           +  + A +  +  G SFYR+  P GS      QVFVA+I  RK   P N N +H+     
Sbjct: 237 AAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQ--PST 296

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
             +  + L  +++FRFLD+A       I +     + PWRLCT+ QV + KIL+ ++PI 
Sbjct: 297 DLVRVKPLLHSNKFRFLDKAC------IKTQGKAMESPWRLCTIEQVHQVKILLSVIPIF 356

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERVFVP 360
             TI   T +AQLQTFS+QQ  +M++     F+IP  S+  IP + + FF+P YE  FVP
Sbjct: 357 ACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVP 416

Query: 361 LARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVF 420
           LARK+TG  +GI  LQRIG GL L   SM  A  VE +R+   ++ N++       +S+F
Sbjct: 417 LARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM-------LSIF 476

Query: 421 WLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNK 480
           W+  QF +FG+ ++FT VGL+EFFY +SS  M+S  TA+ +C+ +FG++ S+V+V+ VN+
Sbjct: 477 WIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNR 536

Query: 481 ASG--------GWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWY------RYKNV 535
            +         GWL  N+LNKD+LD+FYWLL+ LS +NF  YL  + WY       + + 
Sbjct: 537 VTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSATHHSA 587

BLAST of CSPI02G19550 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 396.4 bits (1017), Expect = 3.8e-110
Identity = 214/541 (39.56%), Postives = 330/541 (61.00%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +A+++ A +LV Y   YM+ S +KS   +TNFMGT FLL LLGGF+S+ + S F+  L+ 
Sbjct: 44  LAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLIS 103

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E +G  +LT+QA  P L P  C ++P    CE    S+ A L+ GLYL+ALG  G+K
Sbjct: 104 ASIEFLGLIILTIQARTPSLMPPSC-DSPT---CEEVSGSKAAMLFVGLYLVALGVGGIK 163

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +L + GA+QFD+  P    + S+FFN+F+F L  G++V +T +VW+    GW+W F V 
Sbjct: 164 GSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVS 223

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFV-ASIRNRKLPLPENA--------- 240
           +I++  +I +   G  FYR+ +P GSP+   L+V + AS++        NA         
Sbjct: 224 TIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPS 283

Query: 241 NELHEIRDKEAAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRL--CTVTQVE 300
           N       KE     E+ +   +     RA +  +  + + + +++   RL  CTV QVE
Sbjct: 284 NHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVE 343

Query: 301 ETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFF 360
           + KI+++MLPI   TI +  C+AQL TFS+QQ+ +M+++    KIP  S+P+ P++F+  
Sbjct: 344 DVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMI 403

Query: 361 FIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMV 420
             P Y+ + +P ARK T   TG+ HLQRIG+GLVL+  +MA+A  VE +RK VA    ++
Sbjct: 404 LAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLL 463

Query: 421 DSTEPLPISVFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYF 480
           DS E LP++  W+  Q+   G  D+FTL GLLE+F+ E+ + M+SL+T+++W ++A GY+
Sbjct: 464 DSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYY 523

Query: 481 TSTVVVTVVNKASGG-----WLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYK 525
            S+V+V++VN  +G      WL   ++N+ KLDYFYWL+ VLS  NF  YL  A  Y+Y+
Sbjct: 524 LSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 580

BLAST of CSPI02G19550 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 380.2 bits (975), Expect = 2.8e-105
Identity = 216/527 (40.99%), Postives = 316/527 (59.96%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           M   +V  +L+TY    M+F L+K+   +TNF+GT F+  LLGG++S+ +L  F T ++F
Sbjct: 60  MGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIF 119

Query: 61  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
            F EL G+ LL+VQAH P L+P  C        CE A   +    +  LYL+ALG+  VK
Sbjct: 120 GFVELSGFILLSVQAHLPQLKPPKCNPL-IDQTCEEAKGFKAMIFFMALYLVALGSGCVK 179

Query: 121 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
             + A GADQF    P ++ ++SS+FN   F+ ++G ++ LTL+VW+ T  G D  F V 
Sbjct: 180 PNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVS 239

Query: 181 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPENANELH-EIRDK 240
           + ++   I  +  G  ++R+  P+ S       V VA+I  RKL  P +   LH +    
Sbjct: 240 AAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVA 299

Query: 241 EAAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPI 300
              +P   L  T +FRFLD+A I   DT       ++ PWRLCTVTQVE+ K LI ++PI
Sbjct: 300 NDVVPSSTLPHTPRFRFLDKACIKIQDT-----NTKESPWRLCTVTQVEQVKTLISLVPI 359

Query: 301 ILSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
             STI   T +AQLQTFS+QQ  +M+++    F IP  S+  IP + + F +P Y+   V
Sbjct: 360 FASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLV 419

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+TG  +GI  L RIGIGL L+  SM  A  +E +R++ ++    +       +S+
Sbjct: 420 PFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-------LSI 479

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
           FW+  QF +FG+ ++FT VGL+EFFY +S+ GM+S   A+ +C+ +FG++ S+V+V+VVN
Sbjct: 480 FWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVN 539

Query: 481 KA------SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASW 520
           K       S GWL  N+LNKD+LD FYWLL+VLS+LNF  YL  + W
Sbjct: 540 KITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYE45.7e-11141.40Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q56XQ62.4e-10941.71Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q8H1575.4e-10939.56Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q93VV54.0e-10440.99Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q9SX201.9e-9839.81Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LLC03.7e-27099.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360700 PE=3 SV=1[more]
A0A6J1CR119.1e-26186.00protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1CSN29.1e-26186.00protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1GIY59.1e-25383.18protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 ... [more]
A0A6J1KKU74.5e-25283.24protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE... [more]
Match NameE-valueIdentityDescription
XP_011649593.25.6e-30599.82protein NRT1/ PTR FAMILY 4.5 [Cucumis sativus][more]
XP_038885750.12.4e-27690.02protein NRT1/ PTR FAMILY 4.5-like [Benincasa hispida][more]
KAE8652098.14.2e-26898.15hypothetical protein Csa_022418 [Cucumis sativus][more]
XP_022144216.11.9e-26086.00protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Momordica charantia][more]
XP_022144215.11.9e-26086.00protein NRT1/ PTR FAMILY 4.5-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G27040.24.1e-11241.40Major facilitator superfamily protein [more]
AT1G27040.14.1e-11241.40Major facilitator superfamily protein [more]
AT1G33440.11.7e-11041.71Major facilitator superfamily protein [more]
AT1G69850.13.8e-11039.56nitrate transporter 1:2 [more]
AT1G59740.12.8e-10540.99Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 54..481
e-value: 7.0E-93
score: 311.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..529
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..530
e-value: 6.5E-163
score: 544.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 6..524
NoneNo IPR availablePANTHERPTHR11654:SF110PEPTIDE/NITRATE TRANSPORTERcoord: 1..529

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G19550.1CSPI02G19550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042981 regulation of apoptotic process
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity