CSPI02G19260 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G19260
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	calcium-binding protein-like
Location	Chr2: 17545946 .. 17546740 (-)
RNA-Seq Expression	CSPI02G19260
Synteny	CSPI02G19260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAATTTAATTCTATCACCTCCAAACTCCACATCAAATCCCAAAACACAACCCACATAGTTTTCAACAATGTCTACAAATCCTCTTAAGGATCAGCCTGTCCCAGCCCAAGGTAATTACATGAGTGAACAAGAAGCAAGTAATTTGATAAAAAAGTATGACAAGAATGGTGATTCAATCCTGACCAAAGATGAGCTAACTGCTTTGATCATTGAGCAAGCTGCTTCTCACCATCACCAACAAATTAAGCCTCAAAAAGTTGAACCTAAGGTTAATATTCCTGATGAGAAGGCGTCCTGTGGGCCGTACAAGATGAGGCTTAGTAAAGATGAAATGAGGGAGATTTTCTTAGAGCATGATATTGATGGTGATGGTTATCTCACTAGAAGTGAGCTAGTAAAAGCTTTTAACATGTGTGGTTCCTTCAATTCATTTAGCAAAGCTAACTATGCTTTGAATTTGGCTGATGCTAATGGAGATGGCTTTATTTGTTTGGATGAACTTGATAAAGTTCTTGAGTATGCTGACAGAGTAATCAACAAGACCAAGAAATGAAGAAGTTGTAAAATTTATGTACTTTTCTATGTTATCATCATATTATATATCTAGTACGTACTCATTAAGAAAAACTAGAATAAAGAGTTTGTTCTTGGAGGACGCATGATCAACTCTTACTATATATATATATATATTGCTCCTCTCTTTGGTTTATTGTTGTGTGTATTTGTCATGTTATCGTCTTCTAATATAAATCTTTCCATATTCATAAATATTTACATGACGAATTAATAAAGAT

mRNA sequence

TTAATTTAATTCTATCACCTCCAAACTCCACATCAAATCCCAAAACACAACCCACATAGTTTTCAACAATGTCTACAAATCCTCTTAAGGATCAGCCTGTCCCAGCCCAAGGTAATTACATGAGTGAACAAGAAGCAAGTAATTTGATAAAAAAGTATGACAAGAATGGTGATTCAATCCTGACCAAAGATGAGCTAACTGCTTTGATCATTGAGCAAGCTGCTTCTCACCATCACCAACAAATTAAGCCTCAAAAAGTTGAACCTAAGGTTAATATTCCTGATGAGAAGGCGTCCTGTGGGCCGTACAAGATGAGGCTTAGTAAAGATGAAATGAGGGAGATTTTCTTAGAGCATGATATTGATGGTGATGGTTATCTCACTAGAAGTGAGCTAGTAAAAGCTTTTAACATGTGTGGTTCCTTCAATTCATTTAGCAAAGCTAACTATGCTTTGAATTTGGCTGATGCTAATGGAGATGGCTTTATTTGTTTGGATGAACTTGATAAAGTTCTTGAGTATGCTGACAGAGTAATCAACAAGACCAAGAAATGAAGAAGTTGTAAAATTTATGTACTTTTCTATGTTATCATCATATTATATATCTAGTACGTACTCATTAAGAAAAACTAGAATAAAGAGTTTGTTCTTGGAGGACGCATGATCAACTCTTACTATATATATATATATATTGCTCCTCTCTTTGGTTTATTGTTGTGTGTATTTGTCATGTTATCGTCTTCTAATATAAATCTTTCCATATTCATAAATATTTACATGACGAATTAATAAAGAT

Coding sequence (CDS)

ATGTCTACAAATCCTCTTAAGGATCAGCCTGTCCCAGCCCAAGGTAATTACATGAGTGAACAAGAAGCAAGTAATTTGATAAAAAAGTATGACAAGAATGGTGATTCAATCCTGACCAAAGATGAGCTAACTGCTTTGATCATTGAGCAAGCTGCTTCTCACCATCACCAACAAATTAAGCCTCAAAAAGTTGAACCTAAGGTTAATATTCCTGATGAGAAGGCGTCCTGTGGGCCGTACAAGATGAGGCTTAGTAAAGATGAAATGAGGGAGATTTTCTTAGAGCATGATATTGATGGTGATGGTTATCTCACTAGAAGTGAGCTAGTAAAAGCTTTTAACATGTGTGGTTCCTTCAATTCATTTAGCAAAGCTAACTATGCTTTGAATTTGGCTGATGCTAATGGAGATGGCTTTATTTGTTTGGATGAACTTGATAAAGTTCTTGAGTATGCTGACAGAGTAATCAACAAGACCAAGAAATGA

Protein sequence

MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKPQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK*

Homology

[Close]

BLAST of CSPI02G19260 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 8.2e-05
Identity = 27/72 (37.50%), Postives = 39/72 (54.17%), Query Frame = 0


Query: 73  EKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLA 132
           + AS G       ++EM  +F + D +GDG ++RSEL   F   G   +  +    +  A
Sbjct: 25  DPASGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEA 84

Query: 133 DANGDGFICLDE 145
           DA+GDGFI LDE
Sbjct: 85  DADGDGFISLDE 96

[Close]

BLAST of CSPI02G19260 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-04
Identity = 36/150 (24.00%), Postives = 64/150 (42.67%), Query Frame = 0


Query: 10  PVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALI----IEQAASHHHQQIKPQKV- 69
           P P Q      ++   L +++D NGD  LT+ EL AL+    +       H  +      
Sbjct: 13  PAPQQLRGSQLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDAN 72

Query: 70  -EPKVNIPDEKASCGPYKMR----LSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSF 129
               V   +  A+  P        + + ++ E+F   D DG+G+++ +EL ++    G  
Sbjct: 73  GNGSVEFDELAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQP 132

Query: 130 NSFSKANYALNLADANGDGFICLDELDKVL 150
            +F +    +  AD +GDG I   E   V+
Sbjct: 133 LTFEELTRMMRDADTDGDGVISFKEFAAVM 162

[Close]

BLAST of CSPI02G19260 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 1.8e-04
Identity = 24/83 (28.92%), Postives = 41/83 (49.40%), Query Frame = 0


Query: 66  PKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKA 125
           P   +P   +S  P K R+   E++ +F   D +GDG +T+ EL  +    G +      
Sbjct: 44  PSTMLPSPSSSSAPTK-RIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDL 103

Query: 126 NYALNLADANGDGFICLDELDKV 149
              ++  DANGDG + +DE + +
Sbjct: 104 TQMIHKIDANGDGCVDIDEFESL 125

[Close]

BLAST of CSPI02G19260 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 3.1e-04
Identity = 25/72 (34.72%), Postives = 39/72 (54.17%), Query Frame = 0


Query: 74  KASC-GPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLA 133
           K++C G   M  S  E+ E+F + D +GDG ++ SEL       GS    ++    +  A
Sbjct: 11  KSACSGEVVMEQSVHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEA 70

Query: 134 DANGDGFICLDE 145
           DA+GDG++ L E
Sbjct: 71  DADGDGYVSLQE 82

[Close]

BLAST of CSPI02G19260 vs. ExPASy Swiss-Prot
Match: Q9M8U1 (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 9.0e-04
Identity = 39/152 (25.66%), Postives = 65/152 (42.76%), Query Frame = 0


Query: 9   QPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKP--QKVEP 68
           +P PA+       E   + + +D+N D  LT+ EL +L+          Q+    QK + 
Sbjct: 7   KPAPAKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADR 66

Query: 69  K----VNIPDEKASCGP--YKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 128
                V   +  A   P   K   + D+++ IF   D DG+GY+T +EL  +    G   
Sbjct: 67  NNNGLVEFSEFVALVEPDLVKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHAL 126

Query: 129 SFSKANYALNLADANGDGFICLDELDKVLEYA 153
           +  +    +  AD +GDG I   E  + +  A
Sbjct: 127 TAEELTGMIKEADRDGDGCIDFQEFVQAITSA 158

[Close]

BLAST of CSPI02G19260 vs. ExPASy TrEMBL
Match: A0A0A0LNG6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359930 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.6e-86
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60
           MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK
Sbjct: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60

Query: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120
           PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN
Sbjct: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120

Query: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
           SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK
Sbjct: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 161

[Close]

BLAST of CSPI02G19260 vs. ExPASy TrEMBL
Match: A0A5A7V211 (Calcium-binding protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00200 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 6.8e-71
Identity = 136/161 (84.47%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60
           MSTN L D  VPAQGNYMSEQEA+ LIKKYDKNGDSILTKDELTALI+EQAASHHHQQ+K
Sbjct: 1   MSTNALNDHHVPAQGNYMSEQEANTLIKKYDKNGDSILTKDELTALIMEQAASHHHQQVK 60

Query: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120
           PQKVEPK  +PDEKAS GPYKMRLS+DEMREIFLEHDIDGDG+LTRSEL+KAFNMCGS N
Sbjct: 61  PQKVEPK-KVPDEKASGGPYKMRLSRDEMREIFLEHDIDGDGHLTRSELIKAFNMCGSLN 120

Query: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
           SFSKANYALNLADA+GD +I +DEL+KVL+YADRVIN+ KK
Sbjct: 121 SFSKANYALNLADADGDNYISMDELEKVLDYADRVINRPKK 160

[Close]

BLAST of CSPI02G19260 vs. ExPASy TrEMBL
Match: A0A1S3BDQ7 (calcium-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103488543 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 6.8e-71
Identity = 136/161 (84.47%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60
           MSTN L D  VPAQGNYMSEQEA+ LIKKYDKNGDSILTKDELTALI+EQAASHHHQQ+K
Sbjct: 1   MSTNALNDHHVPAQGNYMSEQEANTLIKKYDKNGDSILTKDELTALIMEQAASHHHQQVK 60

Query: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120
           PQKVEPK  +PDEKAS GPYKMRLS+DEMREIFLEHDIDGDG+LTRSEL+KAFNMCGS N
Sbjct: 61  PQKVEPK-KVPDEKASGGPYKMRLSRDEMREIFLEHDIDGDGHLTRSELIKAFNMCGSLN 120

Query: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
           SFSKANYALNLADA+GD +I +DEL+KVL+YADRVIN+ KK
Sbjct: 121 SFSKANYALNLADADGDNYISMDELEKVLDYADRVINRPKK 160

[Close]

BLAST of CSPI02G19260 vs. ExPASy TrEMBL
Match: A0A6J1KRR4 (calmodulin-A-like OS=Cucurbita maxima OX=3661 GN=LOC111496627 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.9e-33
Identity = 92/163 (56.44%), Postives = 113/163 (69.33%), Query Frame = 0


Query: 1   MSTNP---LKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQ 60
           MS NP     DQP      YMSE +A+NLIKKYDKNGDSILTKDELTA+I+EQA+    Q
Sbjct: 1   MSINPPHVHHDQP------YMSEADANNLIKKYDKNGDSILTKDELTAMILEQAS---QQ 60

Query: 61  QIKPQKV--EPKVN---IPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKA 120
           Q  P+ V  +PK N   +P++K       MRLS+ E+R+IFLEHDIDGDGYL  SEL KA
Sbjct: 61  QGHPKDVVCKPKKNPAKVPEDKG----LHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKA 120

Query: 121 FNMCGSFNSFSKANYALNLADANGDGFICLDELDKVLEYADRV 156
           F+  GS     KA+Y LN ADA+GDG I  +EL K+++YA+RV
Sbjct: 121 FSFIGSMLPLYKAHYGLNYADADGDGRISEEELGKLVDYAERV 150

[Close]

BLAST of CSPI02G19260 vs. ExPASy TrEMBL
Match: A0A6J1GK54 (calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454617 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 7.4e-33
Identity = 83/144 (57.64%), Postives = 106/144 (73.61%), Query Frame = 0


Query: 17  YMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKPQKV--EPK---VNIP 76
           YMSE +A+NLIKKYDKNGDSILTKDELTA+I+EQA+    QQ  P+ +  +PK     +P
Sbjct: 14  YMSEADANNLIKKYDKNGDSILTKDELTAMILEQAS---QQQGPPKDIVCKPKKKPAKVP 73

Query: 77  DEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNL 136
           ++K       MRLS+ E+R+IFLEHDIDGDGYL  SEL KAF+  GS     KA+Y LN 
Sbjct: 74  EDKG----LHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKAFSFIGSMLPLYKAHYGLNY 133

Query: 137 ADANGDGFICLDELDKVLEYADRV 156
           ADA+GDG I  +EL+K+++YA+RV
Sbjct: 134 ADADGDGRISEEELEKLVDYAERV 150

[Close]

BLAST of CSPI02G19260 vs. NCBI nr
Match: XP_004152802.1 (calcium-binding protein [Cucumis sativus] >KGN62524.1 hypothetical protein Csa_022579 [Cucumis sativus])

HSP 1 Score: 327.8 bits (839), Expect = 5.3e-86
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60
           MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK
Sbjct: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60

Query: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120
           PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN
Sbjct: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120

Query: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
           SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK
Sbjct: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 161

[Close]

BLAST of CSPI02G19260 vs. NCBI nr
Match: XP_008445563.1 (PREDICTED: calcium-binding protein-like [Cucumis melo] >KAA0060887.1 calcium-binding protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 276.6 bits (706), Expect = 1.4e-70
Identity = 136/161 (84.47%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIK 60
           MSTN L D  VPAQGNYMSEQEA+ LIKKYDKNGDSILTKDELTALI+EQAASHHHQQ+K
Sbjct: 1   MSTNALNDHHVPAQGNYMSEQEANTLIKKYDKNGDSILTKDELTALIMEQAASHHHQQVK 60

Query: 61  PQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 120
           PQKVEPK  +PDEKAS GPYKMRLS+DEMREIFLEHDIDGDG+LTRSEL+KAFNMCGS N
Sbjct: 61  PQKVEPK-KVPDEKASGGPYKMRLSRDEMREIFLEHDIDGDGHLTRSELIKAFNMCGSLN 120

Query: 121 SFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
           SFSKANYALNLADA+GD +I +DEL+KVL+YADRVIN+ KK
Sbjct: 121 SFSKANYALNLADADGDNYISMDELEKVLDYADRVINRPKK 160

[Close]

BLAST of CSPI02G19260 vs. NCBI nr
Match: XP_038885758.1 (calmodulin-like protein 3 [Benincasa hispida])

HSP 1 Score: 205.3 bits (521), Expect = 4.0e-49
Identity = 114/168 (67.86%), Postives = 132/168 (78.57%), Query Frame = 0


Query: 1   MSTNPLKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQ-- 60
           MSTNPL        GNYMSE+EA++LI+KYDKNGDSILTK+ELTALII+QAASHH  +  
Sbjct: 1   MSTNPLHYHEA-GTGNYMSEKEANSLIQKYDKNGDSILTKEELTALIIDQAASHHQLKPK 60

Query: 61  -----IKPQKVEPKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAF 120
                 KPQKVE KV IPDEK   GP+KMRL++DEMREIFLEHDIDGDG+LTRSEL KA 
Sbjct: 61  KEEVVCKPQKVEAKV-IPDEK---GPFKMRLTRDEMREIFLEHDIDGDGHLTRSELSKAL 120

Query: 121 NMCGSFNSFSKANYALNLADANGDGFICLDELDKVLEYADRVINKTKK 162
             CGS  S  KANYALNLADA+GDG I +DEL+KVL+Y ++ +NK KK
Sbjct: 121 TTCGSLISLHKANYALNLADADGDGLINVDELEKVLDYVEK-LNKLKK 162

[Close]

BLAST of CSPI02G19260 vs. NCBI nr
Match: XP_023002889.1 (calmodulin-A-like [Cucurbita maxima])

HSP 1 Score: 152.1 bits (383), Expect = 4.0e-33
Identity = 92/163 (56.44%), Postives = 113/163 (69.33%), Query Frame = 0


Query: 1   MSTNP---LKDQPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQ 60
           MS NP     DQP      YMSE +A+NLIKKYDKNGDSILTKDELTA+I+EQA+    Q
Sbjct: 1   MSINPPHVHHDQP------YMSEADANNLIKKYDKNGDSILTKDELTAMILEQAS---QQ 60

Query: 61  QIKPQKV--EPKVN---IPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKA 120
           Q  P+ V  +PK N   +P++K       MRLS+ E+R+IFLEHDIDGDGYL  SEL KA
Sbjct: 61  QGHPKDVVCKPKKNPAKVPEDKG----LHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKA 120

Query: 121 FNMCGSFNSFSKANYALNLADANGDGFICLDELDKVLEYADRV 156
           F+  GS     KA+Y LN ADA+GDG I  +EL K+++YA+RV
Sbjct: 121 FSFIGSMLPLYKAHYGLNYADADGDGRISEEELGKLVDYAERV 150

[Close]

BLAST of CSPI02G19260 vs. NCBI nr
Match: XP_022951880.1 (calmodulin-like [Cucurbita moschata])

HSP 1 Score: 150.2 bits (378), Expect = 1.5e-32
Identity = 83/144 (57.64%), Postives = 106/144 (73.61%), Query Frame = 0


Query: 17  YMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKPQKV--EPK---VNIP 76
           YMSE +A+NLIKKYDKNGDSILTKDELTA+I+EQA+    QQ  P+ +  +PK     +P
Sbjct: 14  YMSEADANNLIKKYDKNGDSILTKDELTAMILEQAS---QQQGPPKDIVCKPKKKPAKVP 73

Query: 77  DEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNL 136
           ++K       MRLS+ E+R+IFLEHDIDGDGYL  SEL KAF+  GS     KA+Y LN 
Sbjct: 74  EDKG----LHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKAFSFIGSMLPLYKAHYGLNY 133

Query: 137 ADANGDGFICLDELDKVLEYADRV 156
           ADA+GDG I  +EL+K+++YA+RV
Sbjct: 134 ADADGDGRISEEELEKLVDYAERV 150

[Close]

BLAST of CSPI02G19260 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-05
Identity = 24/83 (28.92%), Postives = 41/83 (49.40%), Query Frame = 0


Query: 66  PKVNIPDEKASCGPYKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKA 125
           P   +P   +S  P K R+   E++ +F   D +GDG +T+ EL  +    G +      
Sbjct: 44  PSTMLPSPSSSSAPTK-RIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDL 103

Query: 126 NYALNLADANGDGFICLDELDKV 149
              ++  DANGDG + +DE + +
Sbjct: 104 TQMIHKIDANGDGCVDIDEFESL 125

[Close]

BLAST of CSPI02G19260 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 45.1 bits (105), Expect = 6.4e-05
Identity = 39/152 (25.66%), Postives = 65/152 (42.76%), Query Frame = 0


Query: 9   QPVPAQGNYMSEQEASNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKP--QKVEP 68
           +P PA+       E   + + +D+N D  LT+ EL +L+          Q+    QK + 
Sbjct: 7   KPAPAKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADR 66

Query: 69  K----VNIPDEKASCGP--YKMRLSKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFN 128
                V   +  A   P   K   + D+++ IF   D DG+GY+T +EL  +    G   
Sbjct: 67  NNNGLVEFSEFVALVEPDLVKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHAL 126

Query: 129 SFSKANYALNLADANGDGFICLDELDKVLEYA 153
           +  +    +  AD +GDG I   E  + +  A
Sbjct: 127 TAEELTGMIKEADRDGDGCIDFQEFVQAITSA 158

[Close]

BLAST of CSPI02G19260 vs. TAIR 10
Match: AT5G17470.1 (EF hand calcium-binding protein family )

HSP 1 Score: 45.1 bits (105), Expect = 6.4e-05
Identity = 35/135 (25.93%), Postives = 63/135 (46.67%), Query Frame = 0


Query: 24  SNLIKKYDKNGDSILTKDELTALIIEQAASHHHQQIKPQKVEPKVNIPD-----EKASCG 83
           + + ++ DKN D  ++ DE    I   + S   ++I     E  V+  +     E ASC 
Sbjct: 4   AEIFERVDKNKDGKISWDEFAEAIRAFSPSITSEEIDNMFREIDVDGDNQIDVAEYASCL 63

Query: 84  PYKMRLSKDE----MREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADA 143
                 +K++    M+E F  +DIDGDG ++ SE+       G   + ++    +   DA
Sbjct: 64  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 123

Query: 144 NGDGFICLDELDKVL 150
           +GDGF+  +E   ++
Sbjct: 124 DGDGFVSFEEFKTMM 138

[Close]

BLAST of CSPI02G19260 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 44.3 bits (103), Expect = 1.1e-04
Identity = 21/60 (35.00%), Postives = 33/60 (55.00%), Query Frame = 0


Query: 85  SKDEMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADANGDGFICLDE 144
           SK+E+R++F   D DGDG ++  EL   F   G + S   A  A+N  D + DG +  ++
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

[Close]

BLAST of CSPI02G19260 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 42.4 bits (98), Expect = 4.2e-04
Identity = 21/74 (28.38%), Postives = 39/74 (52.70%), Query Frame = 0


Query: 88  EMREIFLEHDIDGDGYLTRSELVKAFNMCGSFNSFSKANYALNLADANGDGFICLDELDK 147
           ++  +F   D + DG ++  EL K+F   G   S  +A  A+ L+D +GDG + ++E   
Sbjct: 22  DLEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFAL 81

Query: 148 VLEYADRVINKTKK 162
           +++  D    + KK
Sbjct: 82  LIKGNDEFTEEEKK 95

The following BLAST results are available for this feature:

BLAST of CSPI02G19260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	8.2e-05	37.50	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q6L5F4	1.1e-04	24.00	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O22845	1.8e-04	28.92	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]
O64943	3.1e-04	34.72	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
Q9M8U1	9.0e-04	25.66	Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 ...	[more]

BLAST of CSPI02G19260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNG6	2.6e-86	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359930 PE=4 SV=1	[more]
A0A5A7V211	6.8e-71	84.47	Calcium-binding protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A1S3BDQ7	6.8e-71	84.47	calcium-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103488543 PE=4 SV=1	[more]
A0A6J1KRR4	1.9e-33	56.44	calmodulin-A-like OS=Cucurbita maxima OX=3661 GN=LOC111496627 PE=4 SV=1	[more]
A0A6J1GK54	7.4e-33	57.64	calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454617 PE=4 SV=1	[more]

BLAST of CSPI02G19260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152802.1	5.3e-86	100.00	calcium-binding protein [Cucumis sativus] >KGN62524.1 hypothetical protein Csa_0...	[more]
XP_008445563.1	1.4e-70	84.47	PREDICTED: calcium-binding protein-like [Cucumis melo] >KAA0060887.1 calcium-bin...	[more]
XP_038885758.1	4.0e-49	67.86	calmodulin-like protein 3 [Benincasa hispida]	[more]
XP_023002889.1	4.0e-33	56.44	calmodulin-A-like [Cucurbita maxima]	[more]
XP_022951880.1	1.5e-32	57.64	calmodulin-like [Cucurbita moschata]	[more]

BLAST of CSPI02G19260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G43290.1	1.3e-05	28.92	Calcium-binding EF-hand family protein	[more]
AT3G03000.1	6.4e-05	25.66	EF hand calcium-binding protein family	[more]
AT5G17470.1	6.4e-05	25.93	EF hand calcium-binding protein family	[more]
AT3G50770.1	1.1e-04	35.00	calmodulin-like 41	[more]
AT1G76640.1	4.2e-04	28.38	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 88..116 e-value: 7.7E-4 score: 28.8 coord: 124..152 e-value: 7.0 score: 10.9 coord: 22..50 e-value: 2.1 score: 15.1
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 129..148 e-value: 3.6E-4 score: 19.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 28..113 e-value: 1.2E-7 score: 32.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 120..155 score: 9.520128
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 18..53 score: 10.496468
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 84..119 score: 13.816021
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 88..149 e-value: 2.76905E-9 score: 48.6981
None	No IPR available	GENE3D	1.10.238.10		coord: 5..158 e-value: 1.2E-17 score: 66.0
None	No IPR available	PANTHER	PTHR10891:SF892	CALCIUM-BINDING PROTEIN CML28-RELATED	coord: 22..93
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 22..93
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 97..109
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 133..145
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 15..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G19260.1	CSPI02G19260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding