CSPI02G19170 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G19170
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	calmodulin-like
Location	Chr2: 17512682 .. 17513350 (+)
RNA-Seq Expression	CSPI02G19170
Synteny	CSPI02G19170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACCAAGAGGCTACGCTTTTTAAGGACCCTTCCCTTCCACTTTCTTCTCAACAGTTCTTCAATCGAAGAAAAACGGCGAAGGTTGCGAAGTCGTCTAGGTTTCATCTAAAATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAGTTATCTTTTCCATCCTGCTGTGGAAGGTTCCCTAGTGAGTGTGAGTTGTTGATGTAAAAAAATAATATACAGAAAGGTAATTTGTGTGTTCAAGAGATACCTTCCCCTATTATTGTAAAATGCCTTTCCTGATGGCTGATGCGAAGATGATGATGATAGCATTTCCAATAAAAATAAATTTTATGCTTCATTCTTGCTCTTGCATGATATGATAACAATTTTCTAAATATATGTATATTGTTACAAAATAATAGACGCACTTTATTTTAACAATGTACCAGGA

mRNA sequence

AACCAAGAGGCTACGCTTTTTAAGGACCCTTCCCTTCCACTTTCTTCTCAACAGTTCTTCAATCGAAGAAAAACGGCGAAGGTTGCGAAGTCGTCTAGGTTTCATCTAAAATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAGTTATCTTTTCCATCCTGCTGTGGAAGGTTCCCTAGTGAGTGTGAGTTGTTGATGTAAAAAAATAATATACAGAAAGGTAATTTGTGTGTTCAAGAGATACCTTCCCCTATTATTGTAAAATGCCTTTCCTGATGGCTGATGCGAAGATGATGATGATAGCATTTCCAATAAAAATAAATTTTATGCTTCATTCTTGCTCTTGCATGATATGATAACAATTTTCTAAATATATGTATATTGTTACAAAATAATAGACGCACTTTATTTTAACAATGTACCAGGA

Coding sequence (CDS)

ATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATGTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATTAAAGAATTGTCGAGAGCATTCGGCCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTTTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAG

Protein sequence

MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISEQELDKVVDYAIKCKYNLL*

Homology

[Close]

BLAST of CSPI02G19170 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.1e-04
Identity = 27/59 (45.76%), Postives = 33/59 (55.93%), Query Frame = 0


Query: 17 KEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISEQE 76
          +EEM+ +FR+ D NGDGR+S  EL   F   G        A  +  AD DGDGFIS  E
Sbjct: 38 EEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDE 96

[Close]

BLAST of CSPI02G19170 vs. ExPASy TrEMBL
Match: A0A0A0LKW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.9e-44
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 92
          FLADDDGDGFISEQELDKVVDYAIKCKYNLL
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of CSPI02G19170 vs. ExPASy TrEMBL
Match: A0A5A7UY46 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00260 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.5e-41
Identity = 85/91 (93.41%), Postives = 89/91 (97.80%), Query Frame = 0


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTR LYDKKKSGYKLQKEEMK++FREHDFNGDGRLSIKELSRAFG FGAFFPLYRAAFGL
Sbjct: 1  MTRPLYDKKKSGYKLQKEEMKQIFREHDFNGDGRLSIKELSRAFGSFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 92
          F+ADDDGDGFISEQEL+KVVDYAIKCKYNLL
Sbjct: 61 FVADDDGDGFISEQELEKVVDYAIKCKYNLL 91

[Close]

BLAST of CSPI02G19170 vs. ExPASy TrEMBL
Match: A0A6J1CS20 (calmodulin-like protein 5 OS=Momordica charantia OX=3673 GN=LOC111014054 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.7e-23
Identity = 54/84 (64.29%), Postives = 66/84 (78.57%), Query Frame = 0


Query: 8  KKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDG 67
          KKK   KL +EE+K +FR HD NGDG LSIKELSRAFG  GA  PLYRA  G+  AD DG
Sbjct: 7  KKKPSIKLLREEVKAIFRAHDINGDGHLSIKELSRAFGSLGALLPLYRAVCGILAADADG 66

Query: 68 DGFISEQELDKVVDYAIKCKYNLL 92
          DG I+++EL+KVVDYA+KCKY+++
Sbjct: 67 DGLITDEELEKVVDYAVKCKYDII 90

[Close]

BLAST of CSPI02G19170 vs. ExPASy TrEMBL
Match: A0A5A7UYC0 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00250 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.2e-16
Identity = 42/78 (53.85%), Postives = 62/78 (79.49%), Query Frame = 0


Query: 11 SGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGF 70
          S  +L +E++KE+FREHD +GDGRLS+ E+ +AFG FG+  P Y+A +G+  AD++GDG+
Sbjct: 6  SKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADENGDGY 65

Query: 71 ISEQELDKVVDYAIKCKY 89
          I E+ELDK+VDYA K ++
Sbjct: 66 IEEEELDKLVDYAEKFQH 83

[Close]

BLAST of CSPI02G19170 vs. ExPASy TrEMBL
Match: A0A0A0LRC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.2e-16
Identity = 42/75 (56.00%), Postives = 61/75 (81.33%), Query Frame = 0


Query: 14 KLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISE 73
          +L +E++KE+FREHD +GDGRLS+ E+ +AFG FG+  P Y+A +G+  AD++GDGFI E
Sbjct: 9  QLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDGFIEE 68

Query: 74 QELDKVVDYAIKCKY 89
          +ELDK+VDYA K ++
Sbjct: 69 EELDKLVDYAQKFQH 83

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: KGN62515.1 (hypothetical protein Csa_022644 [Cucumis sativus])

HSP 1 Score: 186.0 bits (471), Expect = 1.4e-43
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 92
          FLADDDGDGFISEQELDKVVDYAIKCKYNLL
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: KAA0060893.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 176.4 bits (446), Expect = 1.1e-40
Identity = 85/91 (93.41%), Postives = 89/91 (97.80%), Query Frame = 0


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTR LYDKKKSGYKLQKEEMK++FREHDFNGDGRLSIKELSRAFG FGAFFPLYRAAFGL
Sbjct: 1  MTRPLYDKKKSGYKLQKEEMKQIFREHDFNGDGRLSIKELSRAFGSFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIKCKYNLL 92
          F+ADDDGDGFISEQEL+KVVDYAIKCKYNLL
Sbjct: 61 FVADDDGDGFISEQELEKVVDYAIKCKYNLL 91

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 171.8 bits (434), Expect = 2.8e-39
Identity = 85/85 (100.00%), Postives = 85/85 (100.00%), Query Frame = 0


Query: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60
          MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKVVDYAIK 86
          FLADDDGDGFISEQELDKVVDYAIK
Sbjct: 61 FLADDDGDGFISEQELDKVVDYAIK 85

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: KAG6585660.1 (Calmodulin-like protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 132.5 bits (332), Expect = 1.9e-27
Identity = 64/87 (73.56%), Postives = 75/87 (86.21%), Query Frame = 0


Query: 5  LYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLAD 64
          LY KKKS  +L++EE+K +FREHD NGDGRLSI+ELSRAFG FGAF PLYRAA  +  AD
Sbjct: 5  LYGKKKSTSRLRREEIKAIFREHDVNGDGRLSIQELSRAFGMFGAFLPLYRAARSMHKAD 64

Query: 65 DDGDGFISEQELDKVVDYAIKCKYNLL 92
           +GDGFI+EQELDKVVDYAIKCKYN++
Sbjct: 65 ANGDGFITEQELDKVVDYAIKCKYNII 91

[Close]

BLAST of CSPI02G19170 vs. NCBI nr
Match: KAG6585659.1 (hypothetical protein SDJN03_18392, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 126.3 bits (316), Expect = 1.3e-25
Identity = 59/86 (68.60%), Postives = 71/86 (82.56%), Query Frame = 0


Query: 6  YDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADD 65
          Y KK S   L +EE+K +F+EHDFNGDGRLSI+ELSRAFG  GAF PLYRA  G+ +AD 
Sbjct: 5  YGKKNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGILIADV 64

Query: 66 DGDGFISEQELDKVVDYAIKCKYNLL 92
          +GDG+I+E ELDKVVDYAIKCKYN++
Sbjct: 65 NGDGYITEHELDKVVDYAIKCKYNII 90

[Close]

BLAST of CSPI02G19170 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 40.8 bits (94), Expect = 6.9e-04
Identity = 21/57 (36.84%), Postives = 32/57 (56.14%), Query Frame = 0


Query: 19 EMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDGDGFISEQE 76
          E+K++F + D NGDG++S+ EL   F   G  +        L   D D DGFI+++E
Sbjct: 20 ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEE 76

The following BLAST results are available for this feature:

BLAST of CSPI02G19170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	5.1e-04	45.76	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CSPI02G19170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKW4	6.9e-44	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358860 PE=4 SV=1	[more]
A0A5A7UY46	5.5e-41	93.41	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1CS20	6.7e-23	64.29	calmodulin-like protein 5 OS=Momordica charantia OX=3673 GN=LOC111014054 PE=4 SV...	[more]
A0A5A7UYC0	1.2e-16	53.85	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LRC1	1.2e-16	56.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1	[more]

BLAST of CSPI02G19170 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62515.1	1.4e-43	100.00	hypothetical protein Csa_022644 [Cucumis sativus]	[more]
KAA0060893.1	1.1e-40	93.41	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
XP_004152805.1	2.8e-39	100.00	probable calcium-binding protein CML10 [Cucumis sativus]	[more]
KAG6585660.1	1.9e-27	73.56	Calmodulin-like protein 5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6585659.1	1.3e-25	68.60	hypothetical protein SDJN03_18392, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CSPI02G19170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73630.1	6.9e-04	36.84	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 19..47 e-value: 2.5E-4 score: 30.4 coord: 53..83 e-value: 80.0 score: 2.4
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 63..80 e-value: 8.6E-4 score: 18.7
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 19..44 e-value: 1.0E-6 score: 27.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 15..50 score: 14.5413
None	No IPR available	GENE3D	1.10.238.10		coord: 7..85 e-value: 3.9E-12 score: 47.6
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 64..76
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 28..40
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 12..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G19170.1	CSPI02G19170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding