CSPI02G15070 (gene) Cucumber (PI 183967) v1

BLAST of CSPI02G15070 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 510.8 bits (1314), Expect = 2.0e-143
Identity = 290/574 (50.52%), Postives = 369/574 (64.29%), Query Frame = 0

Query: 2   VSSNPISLLTFLTLLFLLSPSSSA-VPT-------ADICQSTTSPPFCNSFLPKSPDSIH 61
           +S   + L T   L  L SP  SA +P        ++IC+    P +C S LP  P  I+
Sbjct: 1   MSQKLMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIY 60

Query: 62  SHCRFTLHQALAHARTFLSLVNAQLN------LLPSLSALHDCRCLAEANLDFLFQTFSI 121
           S+ R +L ++L+ AR F+S+++A+L+         ++ AL DC+ LA   +D+L  +   
Sbjct: 61  SYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQT 120

Query: 122 VNSTTTTLPYYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSL 181
            +S T TL    A ++ + +SA ITN  TC EGL S  S  GL   +      D KLY +
Sbjct: 121 ADS-TKTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFN----DTKLYGV 180

Query: 182 YLSLFKMGWV-----SKDLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPG 241
            L+LF  GWV     S+ +  P       FG   G+L LKM+ + RA Y  +        
Sbjct: 181 SLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTR------ 240

Query: 242 ARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIY 301
            R+LLQ++   D + V+ IV V QNG  +FT I AAIAAAP KT  + GYFLI+V AG+Y
Sbjct: 241 -RKLLQSD--ADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLY 300

Query: 302 NETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNT 361
            E V VPK KRYV++IG+G NQT+ITGN++VVDG TTFNSAT  + G  F+GVN+TI NT
Sbjct: 301 EEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNT 360

Query: 362 AGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 421
           AG  K QAVALR   D    Y+C FE YQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV
Sbjct: 361 AGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 420

Query: 422 LQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT-KSYLGRP 481
           LQNCN+Y R P  GQ N +TAQGRTDPNQNTGT+IH CTI+   +LA S  T K+YLGRP
Sbjct: 421 LQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRP 480

Query: 482 WKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGV 541
           WK+YSRTV MQ++ID F++P GW  W G   L+T YYAE+NN+GPG DT+ R +W    V
Sbjct: 481 WKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHV 540

Query: 542 INATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
           INAT ASNFTV+  L G+ W+  T VP+ GGLI+
Sbjct: 541 INATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of CSPI02G15070 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.9e-139
Identity = 284/581 (48.88%), Postives = 371/581 (63.86%), Query Frame = 0

Query: 5   NPISLLTFLT--LLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 64
           +PI +L  L+  L  +L+ S +   ++ IC++T  P +C S  P S  ++  +  F++ +
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRK 62

Query: 65  ALAHARTFLSLV------NAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTT--- 124
           +L+ +R F+  V      NA L+    + AL DCR LA   +D+L  +F  VN T+    
Sbjct: 63  SLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTS 122

Query: 125 --TLPYYDAHEMLSLISAIITNVDTCYEGL---ASLNSAVGLVDKVLEAISFDKKLYSLY 184
              L +  A ++ +L+SA +TN  TC EGL   AS ++   +   V   +  D KL  + 
Sbjct: 123 FKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVS 182

Query: 185 LSLFKMGWVSKDLKAPTF----PKMNHFGAGK-------GQLKLKMSPKDRAYYERLVHR 244
           L+LF  GWV K  K   F    P+       K       G L LKM+ K +A YE L  R
Sbjct: 183 LALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRR 242

Query: 245 NKPPGARRLLQTNYQDDG--IVVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLI 304
               G          DDG  ++++ IV V Q+G  +FTNITAA+AAAP  T  + G+FLI
Sbjct: 243 KLADGD----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLI 302

Query: 305 FVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGV 364
           +V AGIY E + + K KRY+++IG+G NQT++TGN++VVDG TTFNSAT AV    F+ V
Sbjct: 303 YVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAV 362

Query: 365 NLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFI 424
           N+T  NTAG  KHQAVALR  AD    Y+C FE YQDTLYTHSLRQFYRECDVYGTVDFI
Sbjct: 363 NITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFI 422

Query: 425 FGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT- 484
           FGNAAVV QNCN+Y R PM  QFNA+TAQGR+DPNQNTGTSI NCTIK   +L +S  T 
Sbjct: 423 FGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTV 482

Query: 485 KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRA 544
           K+YLGRPWK+YSRTVYMQS+ID F++PVGW+EW+G   L+T YYAE+NN+GPG +T+ R 
Sbjct: 483 KTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRV 542

Query: 545 SW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
           +W    VIN+T A+NFTV+ L     W+  T VPYT GLIS
Sbjct: 543 TWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of CSPI02G15070 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 493.4 bits (1269), Expect = 3.3e-138
Identity = 283/582 (48.63%), Postives = 370/582 (63.57%), Query Frame = 0

Query: 7   ISLLTFLTLLFL-----------LSPSSSAVPTADICQSTTSPPFCNSFLPK-SPDSIHS 66
           +SL  FL  LFL           L PS+S   ++ IC++T  P FC S  P+ S   +  
Sbjct: 2   LSLKLFLVTLFLSLQTLFIASQTLLPSNS---SSTICKTTPDPKFCKSVFPQTSQGDVRE 61

Query: 67  HCRFTLHQALAHARTFLSLVNAQL---NLLPSLS---ALHDCRCLAEANLDFLFQTFSIV 126
           + RF+L ++L  +R F   ++  L   N L S S   AL DCR LA    D+L  +F  V
Sbjct: 62  YGRFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETV 121

Query: 127 NSTTT--TLPYYDAHEMLSLISAIITNVDTCYEGL-ASLNSAVGLVDKVLEAISFDKKLY 186
           N TT+  TL +  A E+ +L+SA +TN  TC +G+  + +S+  + + V   +  D KL+
Sbjct: 122 NITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLF 181

Query: 187 SLYLSLFKMGWVSKDLK-----APTFPKMNHFGA------GKGQLKLKMSPKDRAYYERL 246
           S+ L+LF  GWV K  K     +   PK  H           G L LKM+   RA YE L
Sbjct: 182 SVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL 241

Query: 247 VHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFL 306
                    R+L   +   + ++V+ IV V+QNG  +FT IT A+ +AP KT    GYF+
Sbjct: 242 -------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFV 301

Query: 307 IFVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLG 366
           I+V +G+Y E V++ K KRY+++IG+G N+T++TGN+NVVDG TTFNSAT AV    F+ 
Sbjct: 302 IYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVA 361

Query: 367 VNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDF 426
           VN+T  NTAG  KHQAVA+R SAD    Y+C FE YQDTLYTHSLRQFYRECD+YGTVDF
Sbjct: 362 VNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDF 421

Query: 427 IFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT 486
           IFGNAAVV Q+CN+Y R PM  QFNA+TAQGRTDPNQNTG SIHNCTIK   +L +S  T
Sbjct: 422 IFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYT 481

Query: 487 -KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQR 546
            K+YLGRPWK+YSRTV+MQS+ID  ++PVGW+EW+G   L+T YYAE+NN+G G  T+ R
Sbjct: 482 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 541

Query: 547 ASW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
             W    VIN+T A+NFTV   L GD W+  + VPY  GL+S
Sbjct: 542 VVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of CSPI02G15070 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.3e-113
Identity = 241/560 (43.04%), Postives = 330/560 (58.93%), Query Frame = 0

Query: 19  LSPSSSAVPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 78
           L PS S  P+   C+ST  P  C + L     SP   + + +FT+ Q L  A     ++ 
Sbjct: 71  LPPSQSQSPSL-ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVIT 130

Query: 79  AQLNLLPS---------LSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 138
           +    + S         + A+ DC  L+E ++++L +T +    T   +       + SL
Sbjct: 131 SYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSL 190

Query: 139 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 198
           +S ++TN  TC +GL    S  G    +   +    +LYS+ L L     ++++LK    
Sbjct: 191 LSGVVTNQQTCLDGLVEAKS--GFAAAIGSPMGNLTRLYSISLGLVSHA-LNRNLK---- 250

Query: 199 PKMNHFGAGKGQLKLKMSPKDRAYYERLV---------HRNKPPGARRLLQTNYQDDG-I 258
                F A KG++    +   R   E L+          ++    +R L +      G I
Sbjct: 251 ----RFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSI 310

Query: 259 VVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVL 318
           +V+  V V      +FT IT AIAAAP  T    GYF+I+   G+Y E ++VP  K+ ++
Sbjct: 311 LVSKAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLM 370

Query: 319 LIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVS 378
           L+G+G N+TIITGN NVVDG TT+N ++ AV G  F+ V++T  NTAG  KHQAVALR +
Sbjct: 371 LMGDGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNN 430

Query: 379 ADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSG 438
           A+  + Y C FEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAA + QNCNIYAR PM+ 
Sbjct: 431 AEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAK 490

Query: 439 QFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFI 498
           Q NA+TA GR DPNQNTG SI NCTIKA P+LAA P +  ++LGRPWK YSRTV+MQS+I
Sbjct: 491 QKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYI 550

Query: 499 DSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQL 555
              + PVGW EW+GT+ L+T YY E++N GPG +T+QR  W    ++N   A NFTV   
Sbjct: 551 SDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNF 610

BLAST of CSPI02G15070 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.3e-113
Identity = 239/551 (43.38%), Postives = 326/551 (59.17%), Query Frame = 0

Query: 20  SPSS-SAVPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 79
           SPS  S +P    C+ST  P  C + L     SP   + + +FT+ Q L  AR    ++N
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 80  --AQ-------LNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 139
             AQ        + +  +SA+ DC  LAE ++++L +T +        +       + SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSL 194

Query: 140 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 199
           +  ++TN  TC +GL    S  G    +   +    +LYS+ L L     ++++LK    
Sbjct: 195 LGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKG 254

Query: 200 PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG--IVVNGIVGV 259
            K   FG G   ++  +    +   +          A R L    +  G  I+V   V V
Sbjct: 255 SKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314

Query: 260 DQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGNNQ 319
                 +F  IT A+AAAP  T   +GYF+I+  AG+Y E V++  +KR ++LIG+G N+
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374

Query: 320 TIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYN 379
           TII+GN + +DG TT+NS+T AV G  F+ V++T  NTAG  KHQAVA+R +AD  T Y 
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434

Query: 380 CIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 439
           C FEGYQDTLY HSLRQFYRECD+YGT+DFIFGNAA + QNCNIYAR PM+ Q NA+TA 
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494

Query: 440 GRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFIDSFIDPVG 499
           GRTDPNQ TG SI NCTI A P+LAA P +T ++LGRPWK YSRTVY+QS+I   + PVG
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 554

Query: 500 WKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWA-VGVINATVASNFTVSQLLAGDKWLP 554
           W EW+GT  L+T  Y E++N GPG DTS+R  W+   ++N   A NFTV     GD WLP
Sbjct: 555 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 614

BLAST of CSPI02G15070 vs. ExPASy TrEMBL
Match: A0A5A7TRW1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002210 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.0e-296
Identity = 511/555 (92.07%), Postives = 530/555 (95.50%), Query Frame = 0

Query: 1   MVSSNPISLLTFLTLLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
           MVSSNPIS+LTFLTLLF LSPSSSAVPTA+ICQSTTSPPFCN FLP SP S+HSHCRFTL
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSAVPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFTL 60

Query: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-LPYYD 120
           H AL HARTFLSLVNA LNL PSLS LHDCR LAEANLDFL QTFSIVNSTTTT LPYYD
Sbjct: 61  HHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYYD 120

Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
           AHE L+L+SA+ITN DTCYEGLA L+SAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK
Sbjct: 121 AHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180

Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
           DLKAPT PKM HFG GKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIV+N
Sbjct: 181 DLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVIN 240

Query: 241 GIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300
           GIVGVDQNG YDFTNITAAIAAAP KTTVAKGYFLIFVAAGIYNETVL+PKEKRYVLLIG
Sbjct: 241 GIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
           EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSADN 360

Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLYNCIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
           A+TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI
Sbjct: 421 AVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480

Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540
           DP GWKEWDGT+NLNTSYYAEFNNSGPG DTSQR SWAVGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGDR 540

Query: 541 WLPPTEVPYTGGLIS 555
           WLPPT+VPYTGGLIS
Sbjct: 541 WLPPTDVPYTGGLIS 555

BLAST of CSPI02G15070 vs. ExPASy TrEMBL
Match: A0A1S3C644 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497470 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.0e-296
Identity = 511/555 (92.07%), Postives = 530/555 (95.50%), Query Frame = 0

Query: 1   MVSSNPISLLTFLTLLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
           MVSSNPIS+LTFLTLLF LSPSSSAVPTA+ICQSTTSPPFCN FLP SP S+HSHCRFTL
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSAVPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFTL 60

Query: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-LPYYD 120
           H AL HARTFLSLVNA LNL PSLS LHDCR LAEANLDFL QTFSIVNSTTTT LPYYD
Sbjct: 61  HHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYYD 120

Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
           AHE L+L+SA+ITN DTCYEGLA L+SAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK
Sbjct: 121 AHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180

Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
           DLKAPT PKM HFG GKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIV+N
Sbjct: 181 DLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVIN 240

Query: 241 GIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300
           GIVGVDQNG YDFTNITAAIAAAP KTTVAKGYFLIFVAAGIYNETVL+PKEKRYVLLIG
Sbjct: 241 GIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
           EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSADN 360

Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLYNCIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
           A+TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI
Sbjct: 421 AVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480

Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540
           DP GWKEWDGT+NLNTSYYAEFNNSGPG DTSQR SWAVGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGDR 540

Query: 541 WLPPTEVPYTGGLIS 555
           WLPPT+VPYTGGLIS
Sbjct: 541 WLPPTDVPYTGGLIS 555

BLAST of CSPI02G15070 vs. ExPASy TrEMBL
Match: A0A6J1EJM8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.5e-250
Identity = 432/552 (78.26%), Postives = 479/552 (86.78%), Query Frame = 0

Query: 3   SSNPISLLTFLTLLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 62
           SSNPISLL F +LLFL + SSSAVPT ++CQS TSP FC SFLP SP S+HSHCR+TLH 
Sbjct: 4   SSNPISLLLFFSLLFLFT-SSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLHH 63

Query: 63  ALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHE 122
           AL  ARTF++LV+ QL L PSLSALHDCR LA ANLDF+  T   VN+TT  LP+Y A E
Sbjct: 64  ALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPFYQADE 123

Query: 123 MLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLK 182
           M+SL+SA+ITN+DTC++GLA     +G VDKV++AISFD++LYSLYLSLFKMGWVSK++K
Sbjct: 124 MISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWVSKNMK 183

Query: 183 APTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIV 242
           AP  PK NHFG GKG LKLKMSPKDRAYYERLVH  K P ARRLLQTNY DDGI+VNG+V
Sbjct: 184 APKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVH-GKKPAARRLLQTNYADDGILVNGVV 243

Query: 243 GVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGN 302
            VDQNG YDFT I  AIAAAP K+T A GYFLIFV AGIY E+V+VPK K+YVL+IGEGN
Sbjct: 244 AVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGN 303

Query: 303 NQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTL 362
           N+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  T 
Sbjct: 304 NRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTF 363

Query: 363 YNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALT 422
           Y+CIFEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNALT
Sbjct: 364 YSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALT 423

Query: 423 AQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPV 482
           AQGRTD NQNTG  IH CTIK TP+LAASP TKSYLGRPWK++SRTVYMQSFID+FIDP 
Sbjct: 424 AQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFIDPA 483

Query: 483 GWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLP 542
           GWKEWDGTLNLNTSYYAEF+NSGPG DTSQRA WAVGVINATVA+ FTVS++LAGD+WLP
Sbjct: 484 GWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAGDQWLP 543

Query: 543 PTEVPYTGGLIS 555
           PT VPY+GGLIS
Sbjct: 544 PTAVPYSGGLIS 553

BLAST of CSPI02G15070 vs. ExPASy TrEMBL
Match: A0A6J1I6B8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.4e-248
Identity = 433/553 (78.30%), Postives = 480/553 (86.80%), Query Frame = 0

Query: 3   SSNPISLLTFLTLLFL-LSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLH 62
           SSNPISLLTF +LLFL L  SSSAVPT ++CQS TSP FC SFLP SP S+HSHCR+TLH
Sbjct: 4   SSNPISLLTFFSLLFLFLFTSSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLH 63

Query: 63  QALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAH 122
            AL  ARTF++LV+ QL LLPS SALHDCR LA ANLDF+  T + VN+TT  LP+Y A 
Sbjct: 64  HALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTNALPFYQAD 123

Query: 123 EMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDL 182
           EM+SL+SA+ITNVDTC++GLA   S +GLVDKV++AISFD++LYSLYLSLFKMGWVSK +
Sbjct: 124 EMISLLSAVITNVDTCHDGLA--GSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKTM 183

Query: 183 KAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGI 242
           KAP  PK NHFG G G LKLKMSPKDRAYYERL+H  K P ARRLLQTN  DDGI+VNG+
Sbjct: 184 KAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMH-GKKPAARRLLQTNNADDGILVNGV 243

Query: 243 VGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEG 302
           V VDQNG YDFT I  AIAAAP K+T A GYFLIFV AGIY E+V+VPK K+YVL+IGEG
Sbjct: 244 VAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEG 303

Query: 303 NNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVT 362
           NN+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  T
Sbjct: 304 NNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTT 363

Query: 363 LYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNAL 422
            Y+CIFEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNAL
Sbjct: 364 FYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNAL 423

Query: 423 TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDP 482
           TAQGRTD NQNTG  IH CTIK TP+LAASP TKSYLGRPWK++SRTVYMQSFID+FIDP
Sbjct: 424 TAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFIDP 483

Query: 483 VGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWL 542
            GWKEWDGTLNLNTSYYAEF+NSGPG DTS+RA WAVGVINATVA+ FTVS++LAGD+WL
Sbjct: 484 AGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGDQWL 543

Query: 543 PPTEVPYTGGLIS 555
           PPT VPY+GGLIS
Sbjct: 544 PPTAVPYSGGLIS 553

BLAST of CSPI02G15070 vs. ExPASy TrEMBL
Match: A0A6J1L6X0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.5e-239
Identity = 428/563 (76.02%), Postives = 469/563 (83.30%), Query Frame = 0

Query: 1   MVSSNPISLLTFLTLLFLLSPSSSA----VPTADICQSTTSPPFCNSFLPKSPDSIHSHC 60
           M SS+ I LLTFLTLL+L S SSSA    VP ADIC STTSP FC +FLP S  ++HSHC
Sbjct: 1   MGSSSAIPLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLHQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-- 120
           RFTL  ALAHARTFL+LV+  L L PSL+ALHDCR LA ANLDFL  TF  VN+TTTT  
Sbjct: 61  RFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 --LPYYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLF 180
             LP+Y A EM+SL+SA+ITN +TCYEGL S  SA GLVDKVL+AISFD KLYSLYLSLF
Sbjct: 121 NALPFYKAEEMVSLLSAVITNENTCYEGLMSSGSASGLVDKVLKAISFDTKLYSLYLSLF 180

Query: 181 KMGWVSKDLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTN-Y 240
           KMGWVS D+KAP  PKM HFG GKGQLKL MSPK+RAYYERL+HR+KPP ARRLLQTN  
Sbjct: 181 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKPP-ARRLLQTNSN 240

Query: 241 QDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKE 300
           +DDGI+VNGIV VDQNG YDFT I AAI AAP  +  A GYFLI V AG+YNE+V+VPK 
Sbjct: 241 EDDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMVPKH 300

Query: 301 KRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAV 360
           K+YVL+IGEGNNQTIITGNKNVVDGSTTFNS T  VEGTGF GVNLTITNTAG  K QAV
Sbjct: 301 KKYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKFQAV 360

Query: 361 ALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYAR 420
           ALRVSAD  T Y+CIFEGYQDTLY HSLRQFYRECD+YGT+DFI G+AAVV QNCNIYAR
Sbjct: 361 ALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYAR 420

Query: 421 LPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYM 480
           LPM GQ+  +TAQGRTD NQNTG SIHNCTIK T +LAASP  KSY GRPWK+Y+RTVYM
Sbjct: 421 LPMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYM 480

Query: 481 QSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTV 540
           QSFID+FIDP GW  WDGTLNLNTSYYAEFNNSGPG DTS+RA WAVG+INATVA+NFTV
Sbjct: 481 QSFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNFTV 540

Query: 541 SQLLAGDKWLPPTEVPYTGGLIS 555
           S  L GD+WLPPT VPYTGGLIS
Sbjct: 541 SPFLGGDQWLPPTTVPYTGGLIS 562

BLAST of CSPI02G15070 vs. NCBI nr
Match: XP_004148470.1 (pectinesterase [Cucumis sativus] >KAE8651957.1 hypothetical protein Csa_006047 [Cucumis sativus])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 552/554 (99.64%), Postives = 552/554 (99.64%), Query Frame = 0

Query: 1   MVSSNPISLLTFLTLLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
           MVSSNPISLLTFLTLLFLLSPSSSA PTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL
Sbjct: 1   MVSSNPISLLTFLTLLFLLSPSSSAAPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60

Query: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120
           HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA
Sbjct: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDA 120

Query: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180
           HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD
Sbjct: 121 HEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 180

Query: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240
           LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG
Sbjct: 181 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 240

Query: 241 IVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 300
           IVGVDQNGMYDFTNITAAIAAAP KTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE
Sbjct: 241 IVGVDQNGMYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 300

Query: 301 GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV 360
           GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV
Sbjct: 301 GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV 360

Query: 361 TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 420
           TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA
Sbjct: 361 TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 420

Query: 421 LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 480
           LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID
Sbjct: 421 LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 480

Query: 481 PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW 540
           PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW
Sbjct: 481 PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW 540

Query: 541 LPPTEVPYTGGLIS 555
           LPPTEVPYTGGLIS
Sbjct: 541 LPPTEVPYTGGLIS 554

BLAST of CSPI02G15070 vs. NCBI nr
Match: XP_008457895.1 (PREDICTED: pectinesterase-like [Cucumis melo] >KAA0045890.1 pectinesterase-like [Cucumis melo var. makuwa] >TYK13697.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1026.5 bits (2653), Expect = 8.2e-296
Identity = 511/555 (92.07%), Postives = 530/555 (95.50%), Query Frame = 0

Query: 1   MVSSNPISLLTFLTLLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTL 60
           MVSSNPIS+LTFLTLLF LSPSSSAVPTA+ICQSTTSPPFCN FLP SP S+HSHCRFTL
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSAVPTAEICQSTTSPPFCNFFLPNSPASVHSHCRFTL 60

Query: 61  HQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTT-LPYYD 120
           H AL HARTFLSLVNA LNL PSLS LHDCR LAEANLDFL QTFSIVNSTTTT LPYYD
Sbjct: 61  HHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTTLLPYYD 120

Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
           AHE L+L+SA+ITN DTCYEGLA L+SAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK
Sbjct: 121 AHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180

Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
           DLKAPT PKM HFG GKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIV+N
Sbjct: 181 DLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVIN 240

Query: 241 GIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300
           GIVGVDQNG YDFTNITAAIAAAP KTTVAKGYFLIFVAAGIYNETVL+PKEKRYVLLIG
Sbjct: 241 GIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKEKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
           EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTI+NTAG+AKHQAVALRVSADN
Sbjct: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAVALRVSADN 360

Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLYNCIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
           A+TAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI
Sbjct: 421 AVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480

Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540
           DP GWKEWDGT+NLNTSYYAEFNNSGPG DTSQR SWAVGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTVSQFLAGDR 540

Query: 541 WLPPTEVPYTGGLIS 555
           WLPPT+VPYTGGLIS
Sbjct: 541 WLPPTDVPYTGGLIS 555

BLAST of CSPI02G15070 vs. NCBI nr
Match: XP_038902853.1 (probable pectinesterase/pectinesterase inhibitor 20 [Benincasa hispida])

HSP 1 Score: 950.7 bits (2456), Expect = 5.7e-273
Identity = 472/555 (85.05%), Postives = 507/555 (91.35%), Query Frame = 0

Query: 1   MVSSNPISLLTFLTLLFLLSPSSSA-VPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFT 60
           MVSSN ISLLTFLT LFLL  S SA VPT +IC+STT P FCN+FLP SP S+HSHCRFT
Sbjct: 1   MVSSNSISLLTFLTFLFLLPSSFSAVVPTTEICRSTTFPSFCNAFLPNSPASVHSHCRFT 60

Query: 61  LHQALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYD 120
           LH AL HA+TFL+LV+A L+L P  SALHDCR LAEANLD++ QTFSIVNST+  LPYY 
Sbjct: 61  LHHALTHAQTFLALVDAHLSLFPFPSALHDCRSLAEANLDYITQTFSIVNSTSVALPYYQ 120

Query: 121 AHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSK 180
           AHEM+SL+SA+ITN +TCYEGLA L SA+GLVDKVLEAISFD+KLYSLYLSLFKMGWVSK
Sbjct: 121 AHEMMSLMSAMITNENTCYEGLAGLGSAIGLVDKVLEAISFDRKLYSLYLSLFKMGWVSK 180

Query: 181 DLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVN 240
           ++KAP  PKMNHFG GKGQLKLKMSPKDRAYYERLVH  KP  ARRLLQTNY++DGI+VN
Sbjct: 181 NMKAPILPKMNHFGVGKGQLKLKMSPKDRAYYERLVHSKKPAAARRLLQTNYENDGILVN 240

Query: 241 GIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIG 300
           G+V VDQNG YDFTNI+AAIAAAP KTTV KGYFLIFV AGIYNETVLVPK KRYVLLIG
Sbjct: 241 GVVAVDQNGTYDFTNISAAIAAAPDKTTVTKGYFLIFVTAGIYNETVLVPKNKRYVLLIG 300

Query: 301 EGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADN 360
           EGNNQTII GNKNVVDGSTTFNSATVAVEGTGF GVNLTI+NTAG AKHQAVALRVSADN
Sbjct: 301 EGNNQTIINGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGPAKHQAVALRVSADN 360

Query: 361 VTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFN 420
            TLY+CIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFN
Sbjct: 361 ATLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFN 420

Query: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFI 480
           ALTAQGRTDPNQNTGTSIHNCTIKATPELAA+PATKSYLGRPWK+YSRTVYMQSFID+FI
Sbjct: 421 ALTAQGRTDPNQNTGTSIHNCTIKATPELAANPATKSYLGRPWKEYSRTVYMQSFIDAFI 480

Query: 481 DPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDK 540
           DP GWKEWDGTLNLNTSYYAEFNNSGPG DTS+RA W VGVINATVA++FTVSQ LAGD+
Sbjct: 481 DPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRAPWVVGVINATVANSFTVSQFLAGDQ 540

Query: 541 WLPPTEVPYTGGLIS 555
           WLPPT VPYTGGLIS
Sbjct: 541 WLPPTAVPYTGGLIS 555

BLAST of CSPI02G15070 vs. NCBI nr
Match: KAG6571391.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 876.3 bits (2263), Expect = 1.4e-250
Identity = 436/554 (78.70%), Postives = 483/554 (87.18%), Query Frame = 0

Query: 3   SSNPISLLTFLTLLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 62
           SSNPISLL F +LLFL + SSSAVPT ++CQS TSP FC SFLP SP S+HSHCR+TLH 
Sbjct: 4   SSNPISLLLFFSLLFLFT-SSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLHH 63

Query: 63  ALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHE 122
           AL  ARTF++LV+ QL L PSLSALHDCR LAEANLDF+  T   VN+TT  LP+Y A E
Sbjct: 64  ALVQARTFVTLVDLQLTLFPSLSALHDCRSLAEANLDFISSTVDTVNTTTNALPFYQADE 123

Query: 123 MLSLISAIITNVDTCYEGLA--SLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKD 182
           M+SL+SA+ITNVDTC++GLA     S +GLVDKV++AISFD++LYSLYLSLFKMGWVSK+
Sbjct: 124 MISLLSAVITNVDTCHDGLAGSGSGSGLGLVDKVVKAISFDRQLYSLYLSLFKMGWVSKN 183

Query: 183 LKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNG 242
           +KAP  PK NHFG G G LKLKMSPKDRAYYERLVH  K P ARRLLQTNY DDGI+VNG
Sbjct: 184 MKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVH-GKKPAARRLLQTNYADDGILVNG 243

Query: 243 IVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGE 302
           +V VDQNG YDFT I  AIAAAP K+T A GYFLIFV AGIY E+V+VPK K+YVL+IGE
Sbjct: 244 VVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGE 303

Query: 303 GNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNV 362
           GNN+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  
Sbjct: 304 GNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMT 363

Query: 363 TLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNA 422
           T Y+CIFEGYQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNA
Sbjct: 364 TFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNA 423

Query: 423 LTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFID 482
           LTAQGRTD NQNTG  IH CTIK TP+LAASPATKSYLGRPWK++SRTVYMQSFID+FID
Sbjct: 424 LTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFSRTVYMQSFIDAFID 483

Query: 483 PVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKW 542
           P GWKEWDGTLNLNTSYYAEF+NSGPG DTS+RA WAVGVINATVA+ FTVS++LAGD+W
Sbjct: 484 PAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSEILAGDQW 543

Query: 543 LPPTEVPYTGGLIS 555
           LPPT VPY+GGLIS
Sbjct: 544 LPPTAVPYSGGLIS 555

BLAST of CSPI02G15070 vs. NCBI nr
Match: XP_022928019.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 874.4 bits (2258), Expect = 5.2e-250
Identity = 432/552 (78.26%), Postives = 479/552 (86.78%), Query Frame = 0

Query: 3   SSNPISLLTFLTLLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 62
           SSNPISLL F +LLFL + SSSAVPT ++CQS TSP FC SFLP SP S+HSHCR+TLH 
Sbjct: 4   SSNPISLLLFFSLLFLFT-SSSAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRYTLHH 63

Query: 63  ALAHARTFLSLVNAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHE 122
           AL  ARTF++LV+ QL L PSLSALHDCR LA ANLDF+  T   VN+TT  LP+Y A E
Sbjct: 64  ALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTNALPFYQADE 123

Query: 123 MLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLK 182
           M+SL+SA+ITN+DTC++GLA     +G VDKV++AISFD++LYSLYLSLFKMGWVSK++K
Sbjct: 124 MISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFKMGWVSKNMK 183

Query: 183 APTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDGIVVNGIV 242
           AP  PK NHFG GKG LKLKMSPKDRAYYERLVH  K P ARRLLQTNY DDGI+VNG+V
Sbjct: 184 APKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVH-GKKPAARRLLQTNYADDGILVNGVV 243

Query: 243 GVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGN 302
            VDQNG YDFT I  AIAAAP K+T A GYFLIFV AGIY E+V+VPK K+YVL+IGEGN
Sbjct: 244 AVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKKYVLMIGEGN 303

Query: 303 NQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTL 362
           N+TIITGNKNVVDGSTTFNSATV VEGTGF G NLTITNTAG+ KHQAVALRVSAD  T 
Sbjct: 304 NRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVALRVSADMTTF 363

Query: 363 YNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALT 422
           Y+CIFEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAAVVLQNCNIYARLP+SGQFNALT
Sbjct: 364 YSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLPLSGQFNALT 423

Query: 423 AQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYMQSFIDSFIDPV 482
           AQGRTD NQNTG  IH CTIK TP+LAASP TKSYLGRPWK++SRTVYMQSFID+FIDP 
Sbjct: 424 AQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQSFIDAFIDPA 483

Query: 483 GWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWAVGVINATVASNFTVSQLLAGDKWLP 542
           GWKEWDGTLNLNTSYYAEF+NSGPG DTSQRA WAVGVINATVA+ FTVS++LAGD+WLP
Sbjct: 484 GWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSEILAGDQWLP 543

Query: 543 PTEVPYTGGLIS 555
           PT VPY+GGLIS
Sbjct: 544 PTAVPYSGGLIS 553

BLAST of CSPI02G15070 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 510.8 bits (1314), Expect = 1.4e-144
Identity = 290/574 (50.52%), Postives = 369/574 (64.29%), Query Frame = 0

Query: 2   VSSNPISLLTFLTLLFLLSPSSSA-VPT-------ADICQSTTSPPFCNSFLPKSPDSIH 61
           +S   + L T   L  L SP  SA +P        ++IC+    P +C S LP  P  I+
Sbjct: 1   MSQKLMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIY 60

Query: 62  SHCRFTLHQALAHARTFLSLVNAQLN------LLPSLSALHDCRCLAEANLDFLFQTFSI 121
           S+ R +L ++L+ AR F+S+++A+L+         ++ AL DC+ LA   +D+L  +   
Sbjct: 61  SYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQT 120

Query: 122 VNSTTTTLPYYDAHEMLSLISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSL 181
            +S T TL    A ++ + +SA ITN  TC EGL S  S  GL   +      D KLY +
Sbjct: 121 ADS-TKTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFN----DTKLYGV 180

Query: 182 YLSLFKMGWV-----SKDLKAPTFPKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPG 241
            L+LF  GWV     S+ +  P       FG   G+L LKM+ + RA Y  +        
Sbjct: 181 SLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTR------ 240

Query: 242 ARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIY 301
            R+LLQ++   D + V+ IV V QNG  +FT I AAIAAAP KT  + GYFLI+V AG+Y
Sbjct: 241 -RKLLQSD--ADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLY 300

Query: 302 NETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNT 361
            E V VPK KRYV++IG+G NQT+ITGN++VVDG TTFNSAT  + G  F+GVN+TI NT
Sbjct: 301 EEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNT 360

Query: 362 AGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 421
           AG  K QAVALR   D    Y+C FE YQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV
Sbjct: 361 AGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 420

Query: 422 LQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT-KSYLGRP 481
           LQNCN+Y R P  GQ N +TAQGRTDPNQNTGT+IH CTI+   +LA S  T K+YLGRP
Sbjct: 421 LQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRP 480

Query: 482 WKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGV 541
           WK+YSRTV MQ++ID F++P GW  W G   L+T YYAE+NN+GPG DT+ R +W    V
Sbjct: 481 WKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHV 540

Query: 542 INATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
           INAT ASNFTV+  L G+ W+  T VP+ GGLI+
Sbjct: 541 INATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of CSPI02G15070 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 496.5 bits (1277), Expect = 2.8e-140
Identity = 284/581 (48.88%), Postives = 371/581 (63.86%), Query Frame = 0

Query: 5   NPISLLTFLT--LLFLLSPSSSAVPTADICQSTTSPPFCNSFLPKSPDSIHSHCRFTLHQ 64
           +PI +L  L+  L  +L+ S +   ++ IC++T  P +C S  P S  ++  +  F++ +
Sbjct: 3   SPIFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRK 62

Query: 65  ALAHARTFLSLV------NAQLNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTT--- 124
           +L+ +R F+  V      NA L+    + AL DCR LA   +D+L  +F  VN T+    
Sbjct: 63  SLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTS 122

Query: 125 --TLPYYDAHEMLSLISAIITNVDTCYEGL---ASLNSAVGLVDKVLEAISFDKKLYSLY 184
              L +  A ++ +L+SA +TN  TC EGL   AS ++   +   V   +  D KL  + 
Sbjct: 123 FKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVS 182

Query: 185 LSLFKMGWVSKDLKAPTF----PKMNHFGAGK-------GQLKLKMSPKDRAYYERLVHR 244
           L+LF  GWV K  K   F    P+       K       G L LKM+ K +A YE L  R
Sbjct: 183 LALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRR 242

Query: 245 NKPPGARRLLQTNYQDDG--IVVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLI 304
               G          DDG  ++++ IV V Q+G  +FTNITAA+AAAP  T  + G+FLI
Sbjct: 243 KLADGD----SNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLI 302

Query: 305 FVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGV 364
           +V AGIY E + + K KRY+++IG+G NQT++TGN++VVDG TTFNSAT AV    F+ V
Sbjct: 303 YVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAV 362

Query: 365 NLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFI 424
           N+T  NTAG  KHQAVALR  AD    Y+C FE YQDTLYTHSLRQFYRECDVYGTVDFI
Sbjct: 363 NITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFI 422

Query: 425 FGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT- 484
           FGNAAVV QNCN+Y R PM  QFNA+TAQGR+DPNQNTGTSI NCTIK   +L +S  T 
Sbjct: 423 FGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTV 482

Query: 485 KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRA 544
           K+YLGRPWK+YSRTVYMQS+ID F++PVGW+EW+G   L+T YYAE+NN+GPG +T+ R 
Sbjct: 483 KTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRV 542

Query: 545 SW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
           +W    VIN+T A+NFTV+ L     W+  T VPYT GLIS
Sbjct: 543 TWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of CSPI02G15070 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 493.4 bits (1269), Expect = 2.3e-139
Identity = 283/582 (48.63%), Postives = 370/582 (63.57%), Query Frame = 0

Query: 7   ISLLTFLTLLFL-----------LSPSSSAVPTADICQSTTSPPFCNSFLPK-SPDSIHS 66
           +SL  FL  LFL           L PS+S   ++ IC++T  P FC S  P+ S   +  
Sbjct: 2   LSLKLFLVTLFLSLQTLFIASQTLLPSNS---SSTICKTTPDPKFCKSVFPQTSQGDVRE 61

Query: 67  HCRFTLHQALAHARTFLSLVNAQL---NLLPSLS---ALHDCRCLAEANLDFLFQTFSIV 126
           + RF+L ++L  +R F   ++  L   N L S S   AL DCR LA    D+L  +F  V
Sbjct: 62  YGRFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETV 121

Query: 127 NSTTT--TLPYYDAHEMLSLISAIITNVDTCYEGL-ASLNSAVGLVDKVLEAISFDKKLY 186
           N TT+  TL +  A E+ +L+SA +TN  TC +G+  + +S+  + + V   +  D KL+
Sbjct: 122 NITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLF 181

Query: 187 SLYLSLFKMGWVSKDLK-----APTFPKMNHFGA------GKGQLKLKMSPKDRAYYERL 246
           S+ L+LF  GWV K  K     +   PK  H           G L LKM+   RA YE L
Sbjct: 182 SVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESL 241

Query: 247 VHRNKPPGARRLLQTNYQDDGIVVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFL 306
                    R+L   +   + ++V+ IV V+QNG  +FT IT A+ +AP KT    GYF+
Sbjct: 242 -------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFV 301

Query: 307 IFVAAGIYNETVLVPKEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLG 366
           I+V +G+Y E V++ K KRY+++IG+G N+T++TGN+NVVDG TTFNSAT AV    F+ 
Sbjct: 302 IYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVA 361

Query: 367 VNLTITNTAGSAKHQAVALRVSADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDF 426
           VN+T  NTAG  KHQAVA+R SAD    Y+C FE YQDTLYTHSLRQFYRECD+YGTVDF
Sbjct: 362 VNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDF 421

Query: 427 IFGNAAVVLQNCNIYARLPMSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASPAT 486
           IFGNAAVV Q+CN+Y R PM  QFNA+TAQGRTDPNQNTG SIHNCTIK   +L +S  T
Sbjct: 422 IFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYT 481

Query: 487 -KSYLGRPWKQYSRTVYMQSFIDSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQR 546
            K+YLGRPWK+YSRTV+MQS+ID  ++PVGW+EW+G   L+T YYAE+NN+G G  T+ R
Sbjct: 482 VKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 541

Query: 547 ASW-AVGVINATVASNFTVSQLLAGDKWLPPTEVPYTGGLIS 555
             W    VIN+T A+NFTV   L GD W+  + VPY  GL+S
Sbjct: 542 VVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of CSPI02G15070 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 409.5 bits (1051), Expect = 4.4e-114
Identity = 241/560 (43.04%), Postives = 330/560 (58.93%), Query Frame = 0

Query: 19  LSPSSSAVPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 78
           L PS S  P+   C+ST  P  C + L     SP   + + +FT+ Q L  A     ++ 
Sbjct: 71  LPPSQSQSPSL-ACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVIT 130

Query: 79  AQLNLLPS---------LSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 138
           +    + S         + A+ DC  L+E ++++L +T +    T   +       + SL
Sbjct: 131 SYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTAALVEHVNSL 190

Query: 139 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 198
           +S ++TN  TC +GL    S  G    +   +    +LYS+ L L     ++++LK    
Sbjct: 191 LSGVVTNQQTCLDGLVEAKS--GFAAAIGSPMGNLTRLYSISLGLVSHA-LNRNLK---- 250

Query: 199 PKMNHFGAGKGQLKLKMSPKDRAYYERLV---------HRNKPPGARRLLQTNYQDDG-I 258
                F A KG++    +   R   E L+          ++    +R L +      G I
Sbjct: 251 ----RFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSI 310

Query: 259 VVNGIVGVDQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVL 318
           +V+  V V      +FT IT AIAAAP  T    GYF+I+   G+Y E ++VP  K+ ++
Sbjct: 311 LVSKAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLM 370

Query: 319 LIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVS 378
           L+G+G N+TIITGN NVVDG TT+N ++ AV G  F+ V++T  NTAG  KHQAVALR +
Sbjct: 371 LMGDGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNN 430

Query: 379 ADNVTLYNCIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSG 438
           A+  + Y C FEGYQDTLY HSLRQFYRECD+YGTVDFIFGNAA + QNCNIYAR PM+ 
Sbjct: 431 AEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAK 490

Query: 439 QFNALTAQGRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFI 498
           Q NA+TA GR DPNQNTG SI NCTIKA P+LAA P +  ++LGRPWK YSRTV+MQS+I
Sbjct: 491 QKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYI 550

Query: 499 DSFIDPVGWKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASW-AVGVINATVASNFTVSQL 555
              + PVGW EW+GT+ L+T YY E++N GPG +T+QR  W    ++N   A NFTV   
Sbjct: 551 SDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNF 610

BLAST of CSPI02G15070 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 409.5 bits (1051), Expect = 4.4e-114
Identity = 239/551 (43.38%), Postives = 326/551 (59.17%), Query Frame = 0

Query: 20  SPSS-SAVPTADICQSTTSPPFCNSFL---PKSPDSIHSHCRFTLHQALAHARTFLSLVN 79
           SPS  S +P    C+ST  P  C + L     SP   + + +FT+ Q L  AR    ++N
Sbjct: 75  SPSQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVIN 134

Query: 80  --AQ-------LNLLPSLSALHDCRCLAEANLDFLFQTFSIVNSTTTTLPYYDAHEMLSL 139
             AQ        + +  +SA+ DC  LAE ++++L +T +        +       + SL
Sbjct: 135 RFAQRVEADPGTSTVEEVSAVADCGELAELSVEYL-ETVTEELKAAELMTAALVDRVTSL 194

Query: 140 ISAIITNVDTCYEGLASLNSAVGLVDKVLEAISFDKKLYSLYLSLFKMGWVSKDLKAPTF 199
           +  ++TN  TC +GL    S  G    +   +    +LYS+ L L     ++++LK    
Sbjct: 195 LGGVVTNQQTCLDGLVDAKS--GFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKG 254

Query: 200 PKMNHFGAGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTNYQDDG--IVVNGIVGV 259
            K   FG G   ++  +    +   +          A R L    +  G  I+V   V V
Sbjct: 255 SKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTV 314

Query: 260 DQNGMYDFTNITAAIAAAPIKTTVAKGYFLIFVAAGIYNETVLVPKEKRYVLLIGEGNNQ 319
                 +F  IT A+AAAP  T   +GYF+I+  AG+Y E V++  +KR ++LIG+G N+
Sbjct: 315 GPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINK 374

Query: 320 TIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTITNTAGSAKHQAVALRVSADNVTLYN 379
           TII+GN + +DG TT+NS+T AV G  F+ V++T  NTAG  KHQAVA+R +AD  T Y 
Sbjct: 375 TIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYR 434

Query: 380 CIFEGYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNIYARLPMSGQFNALTAQ 439
           C FEGYQDTLY HSLRQFYRECD+YGT+DFIFGNAA + QNCNIYAR PM+ Q NA+TA 
Sbjct: 435 CSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAH 494

Query: 440 GRTDPNQNTGTSIHNCTIKATPELAASP-ATKSYLGRPWKQYSRTVYMQSFIDSFIDPVG 499
           GRTDPNQ TG SI NCTI A P+LAA P +T ++LGRPWK YSRTVY+QS+I   + PVG
Sbjct: 495 GRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVG 554

Query: 500 WKEWDGTLNLNTSYYAEFNNSGPGCDTSQRASWA-VGVINATVASNFTVSQLLAGDKWLP 554
           W EW+GT  L+T  Y E++N GPG DTS+R  W+   ++N   A NFTV     GD WLP
Sbjct: 555 WLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLP 614