CSPI02G09060 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G09060
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	cytochrome b5-like
Location	Chr2: 8760741 .. 8763966 (-)
RNA-Seq Expression	CSPI02G09060
Synteny	CSPI02G09060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGACTTCCAAATGAAACACATTGTCTAACACTGATGGCCAAACCAAGTGATCAAGTGTCGGCTGTTTCCTGTTTGAACACAAACCAAGACATCACTGAGGACCAATAACTCACACCCACCACCGGAATTCAAATTCAATTCAATTCAATGCTGCTTCCCTGTCTCCCACTTCGACGCATTTACTCGCTACCCTTTTTATTTCTGCACCGCCCTCACTAGTTTCTGCACTCTTTTTTGGTTCTCTCATTTCTCCGCCGTCTCCGGGCAGATTGGCAAATGGGTGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTTTCTTCATACCCTTTTCTTTGTTTTTGTTTTTGGGATTTCTTCTTTCTATCATGCCTGCAGGAGTTTGATTCTCTGAAGTTGTTTGTTCTTCTTTGCTCTTGAAATCTCACATGCATTCTGGACAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCAAGCTACTGCATGGTGAGCTTTGCCGATGATGTTGTATTGGGCTGGCTAATGAAGGATTTTAGTTGCAGAGTACAGTGTAAATTGAACTATTCCTTTGCCTTTTTTCTTGGGAATGCTAAGAGCTTCAGGCTTCATTTTGTAGCTTTTAAATCATGGCAGCAAACGGTGCTTGAATACGAGGGAAATTAGTGTTTGGTGTATTTAAAACTTAAACGTGAGAAGTAGGATAAAATTTGAGTTTCTGTTGCTGGTTAGAAGGCATCATATCCTTTGGCATTATAATCAGGTTCACCAAACATTAATTACAGTTAGCATTCCCTCTAGTCAGCCACTTTTATCGTTGGCAAAATCAATTTCAAGCGCAGCCTCAATCGTTGAAGAGTGGTGTTTCATCCTCCCCGGGGCTTAGAGGATTGATTTTTGGTTGAGTTAATAAGTTTTGGAAAGGAATGGGTTCTATAAATGTGTACTCATATCACGTATATTTGGTTTAGGAGTTTTTGATCTATAAAGTATTATAAAGTGGATTTTATAAGCCTGAGAATGTGTAACATTGGTTAATGTAGTTCATGAGGTTAACAAACAAATTTGCATACGAAGATAAACAAACTCACATGATGGCAAGCATAGATTTGGCTTCTTAATTTCGGAATATACATTTCTTGCTCTGCTGTGCTCACCTGACCAAGTATATAAGACTGAACTGGAGATTTATGATTTATAAGATCTTAAAAAATGCGCTGTGTTTAGTGTCTTGAAGGGACTTATTTTGTGTCTTTGATTCCTTTAGGACCAGGATCTCATTGTATGGATAGAATCTAAGCCTTATTCATGTTTGGCAGTCCTGTTATGAAACAAATGCTTTCATATTTTTGCCAATTCACTGACAGGAATATCAGTGTAGAAAAACTTTGTTTCAAGAGCGGTTAAATATGAAGATCACTGACTTGAAAATGAACTGATCCAAAGATCTCATTTTATTTTTGTGTGTGTGAATGCTCTTATCTACCGAGGGAGGTCTTATGCATATACTATGTGAGAACTGGACTCCAGTGGGGGTTGGACGTCCAACACTTCGTTCAAACCTAACCAATAAAAAAATCAGACGTCTGGGGGATGAATAGTCAACATACATCGTTGTTATGCTGTCTGGTGGGTCAATGTCTGTCACTGCAGACCACATCTACCATAAAAAAATTCTTTCATTCTGATTCTTCTGAATCCAATCGATGATTTTTTTCTTTGGCATCCATAATATGATCTCAAAGCTATATCCTGAAGCCTCAGTCTGTTGAATTTGCACATTCTATTGATGTTAAAAATTTCCTGCCAGCCATCAGACTTACGTCCGGCCCTACTAATCTTCAAGATGGCTTCATCCACACATTGGAAAGATTCATGACATACAAAGAGTTTTAAAATATTGACTTCCCCTACCTCGGCTTTAAAGTTGAACCCAGATATTTCAAGTAGTTCTCTAGAATTAAAGTCCTTTACACTAAAGCCATCCTTGGGGTAAAATCAGAAATTTAGTGTCCAATTAGTAGTGGGAAGCTCTTGCTGGCTGTACTTGTTCATCACTCGTTCTTATCAATGTAATGTGAAATCTCTTTCTTGACCTACCATATCAATAAAGGATAAAGATACTCTAGGAATGCTAATTGTGTTGGAGAATGTCAAGTACTAGTTTCTCAACAACCAAAACTAGATAACCAAGACCATTAGCCAACTCAAGTTTCTTAAGGTTCTAAGTGTAAAGTTAGAAGCAGGAGGATAACATGAAAATGCATGAAACTTCCCTCAGCTTTGCCCAATTCCTACTCGGAAGTGAATATAAATTATATCTTCTCAAAGAACAACACTTGTGCATTTCTACTATTGTTTTTCTTCTAAAATTTCTTGAATTATAGTAATAGAAAGTCGAATAATTTTGATTTTCTCACTTGTACTATGAGTTCAGAATGGGATCAACAAGCTTTCAGTTGTTTTGGCAGTTGGCCTGGAGTTAAACTATATGAATCATGCACAGATTTTATCTTTGATGGGTATTGGTTTTTAGCTTGAGGTTACCATTTTGTCCATCTTTGATTGTCTCAACAGGTTTACAATGTAACCGAGTTCTTGGAAGATCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAATAAGTCCTCCAGTCATACAGCATTAGTTATTTACAATGTACATTGAGTGTTTGTGCATCGTTTACACACTGTAATTTTTATGACCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAACGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACACTCCCAGAACGGTTGACATACACAGGTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGAGGTTCTGGTTGAGATATTATGTGATGTAGTCTTATGATCAATTACAAACCGCTGCATTGTGTTTGTGAAACTGAGAAATTTTCTACTCTAATGCTCTACTTTTTTTTTCTCTTCCTTTTCTGTGTCTTTTACATTTTAGTTCTGCTATCTGATTGGTAAAGTAAATATAATTT

mRNA sequence

GTGGACTTCCAAATGAAACACATTGTCTAACACTGATGGCCAAACCAAGTGATCAAGTGTCGGCTGTTTCCTGTTTGAACACAAACCAAGACATCACTGAGGACCAATAACTCACACCCACCACCGGAATTCAAATTCAATTCAATTCAATGCTGCTTCCCTGTCTCCCACTTCGACGCATTTACTCGCTACCCTTTTTATTTCTGCACCGCCCTCACTAGTTTCTGCACTCTTTTTTGGTTCTCTCATTTCTCCGCCGTCTCCGGGCAGATTGGCAAATGGGTGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTACAATGTAACCGAGTTCTTGGAAGATCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAACGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACACTCCCAGAACGGTTGACATACACAGGTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGAGGTTCTGGTTGAGATATTATGTGATGTAGTCTTATGATCAATTACAAACCGCTGCATTGTGTTTGTGAAACTGAGAAATTTTCTACTCTAATGCTCTACTTTTTTTTTCTCTTCCTTTTCTGTGTCTTTTACATTTTAGTTCTGCTATCTGATTGGTAAAGTAAATATAATTT

Coding sequence (CDS)

ATGGGTGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTACAATGTAACCGAGTTCTTGGAAGATCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAACGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACACTCCCAGAACGGTTGACATACACAGGTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGA

Protein sequence

MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAILGLAVGIRFYNKSS*

Homology

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BLAST of CSPI02G09060 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 9.7e-60
Identity = 105/133 (78.95%), Postives = 120/133 (90.23%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG + K+FTL+EVS+HN   DCW++INGKVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE M +YYVGEID +T+P+++ YT PKQPHYNQDKT+EFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKS 134
           LGLAVGIR Y KS
Sbjct: 121 LGLAVGIRIYTKS 133

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BLAST of CSPI02G09060 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 4.1e-58
Identity = 102/134 (76.12%), Postives = 115/134 (85.82%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG  K+FTLAEVS HNN KDCWLII+GKVYNVT+FLEDHPGG EVLLS TGKDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           D+GHS SAR  + +YYVG+ID ST+P ++ YT PKQPHYNQDKTTEFI+KLLQFLVPL I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LGLAVGIRFYNKSS 135
           LG+A G+ FY K S
Sbjct: 121 LGVAFGVHFYTKQS 134

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BLAST of CSPI02G09060 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.5e-57
Identity = 100/134 (74.63%), Postives = 117/134 (87.31%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG+ K+FTLAEVSQHNN KDCWL+I+GKVY+VT+FL+DHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS SAR  + +YYVG+ID +T+P +  YT P QPHYNQDKT+EF++KLLQFLVPL I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAVGIRFYNKSS 135
           LG+A GIRFY K S
Sbjct: 121 LGVAFGIRFYTKQS 134

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BLAST of CSPI02G09060 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.2e-54
Identity = 97/130 (74.62%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 5   GKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGH 64
           GK+FTLAEV+QHNN KDCWLIINGKVY+VT+FLEDHPGGD+VLLS TGKDATDDFED+GH
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGH 61

Query: 65  SESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAILGLA 124
           S SA+  + +YYVG+ID S++P ++ YT PKQP YN DKT EF+IKLLQFLVPL IL  A
Sbjct: 62  SSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGA 121

Query: 125 VGIRFYNKSS 135
           +GIRFY KSS
Sbjct: 122 IGIRFYTKSS 131

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BLAST of CSPI02G09060 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 3.9e-53
Identity = 96/128 (75.00%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 6   KLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 65
           K++TL EV++HN+  DCWLII GKVYNV++FLEDHPGGD+VLLS TGKDATDDFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  ESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAILGLAV 125
            +AR  M +YYVG+ID ST+P R  Y  PKQPHYNQDKT EFIIK+LQFLVPLAILGLAV
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 GIRFYNKS 134
            IR Y KS
Sbjct: 127 AIRIYTKS 134

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BLAST of CSPI02G09060 vs. ExPASy TrEMBL
Match: A0A0A0LHS9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138200 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.1e-72
Identity = 133/134 (99.25%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]

BLAST of CSPI02G09060 vs. ExPASy TrEMBL
Match: A0A5D3CSF5 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00160 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.3e-70
Identity = 129/134 (96.27%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE MSKYY+GEIDIST+PE+LTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]

BLAST of CSPI02G09060 vs. ExPASy TrEMBL
Match: A0A1S3C5R9 (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.3e-70
Identity = 129/134 (96.27%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE MSKYY+GEIDIST+PE+LTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]

BLAST of CSPI02G09060 vs. ExPASy TrEMBL
Match: A0A6J1CAX1 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 8.5e-67
Identity = 121/134 (90.30%), Postives = 130/134 (97.01%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTLAEVSQHN PKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS+SARETM+KYYVGEIDIST+PE++TY+  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFY KS+
Sbjct: 170 LGLAVGIRFYTKST 183

[Close]

BLAST of CSPI02G09060 vs. ExPASy TrEMBL
Match: A0A6J1H4S5 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111460140 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.6e-65
Identity = 121/134 (90.30%), Postives = 129/134 (96.27%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK+FTLAEVSQHN  +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARETM+KYYVG+IDIST+PERLTYT  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESARETMNKYYVGDIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFY+KS+
Sbjct: 121 LGLAVGIRFYSKST 134

[Close]

BLAST of CSPI02G09060 vs. NCBI nr
Match: XP_004138957.1 (cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cucumis sativus])

HSP 1 Score: 280.0 bits (715), Expect = 1.1e-71
Identity = 133/134 (99.25%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]

BLAST of CSPI02G09060 vs. NCBI nr
Match: XP_008457249.1 (PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [Cucumis melo var. makuwa] >TYK14823.1 cytochrome b5-like [Cucumis melo var. makuwa])

HSP 1 Score: 275.4 bits (703), Expect = 2.6e-70
Identity = 129/134 (96.27%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE MSKYY+GEIDIST+PE+LTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]

BLAST of CSPI02G09060 vs. NCBI nr
Match: XP_022138846.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 262.7 bits (670), Expect = 1.8e-66
Identity = 121/134 (90.30%), Postives = 130/134 (97.01%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTLAEVSQHN PKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS+SARETM+KYYVGEIDIST+PE++TY+  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFY KS+
Sbjct: 170 LGLAVGIRFYTKST 183

[Close]

BLAST of CSPI02G09060 vs. NCBI nr
Match: XP_022959033.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 258.5 bits (659), Expect = 3.3e-65
Identity = 121/134 (90.30%), Postives = 129/134 (96.27%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK+FTLAEVSQHN  +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARETM+KYYVG+IDIST+PERLTYT  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESARETMNKYYVGDIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFY+KS+
Sbjct: 121 LGLAVGIRFYSKST 134

[Close]

BLAST of CSPI02G09060 vs. NCBI nr
Match: XP_023006481.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 258.1 bits (658), Expect = 4.3e-65
Identity = 121/134 (90.30%), Postives = 129/134 (96.27%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK+FTLAEVSQHN  +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE+M+KYYVGEIDIST+PERLTYT  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFY+KS+
Sbjct: 121 LGLAVGIRFYSKST 134

[Close]

BLAST of CSPI02G09060 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 230.7 bits (587), Expect = 6.9e-61
Identity = 105/133 (78.95%), Postives = 120/133 (90.23%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG + K+FTL+EVS+HN   DCW++INGKVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE M +YYVGEID +T+P+++ YT PKQPHYNQDKT+EFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKS 134
           LGLAVGIR Y KS
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of CSPI02G09060 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 196.1 bits (497), Expect = 1.9e-50
Identity = 88/132 (66.67%), Postives = 108/132 (81.82%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           M  D K+ +  EVS+HN  KDCWLII+GKVY+VT F++DHPGGDEVLLS TGKDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS++AR+ M KY++GEID S++P   TY  P+QP YNQDKT EFIIK+LQFLVP+ I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAVGIRFYNK 133
           LGLA+ +R Y K
Sbjct: 121 LGLALVVRHYTK 132

[Close]

BLAST of CSPI02G09060 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 190.7 bits (483), Expect = 7.9e-49
Identity = 85/135 (62.96%), Postives = 112/135 (82.96%), Query Frame = 0


Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTL+EVSQH++ KDCW++I+GKVY+VT+FL+DHPGGDEV+L+ TGKDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPH--YNQDKTTEFIIKLLQFLVPL 120
           DVGHS +A+  + +YYVG+ID +T+P +  +  P        QDK+++F+IKLLQFLVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 AILGLAVGIRFYNKS 134
            ILGLA GIR+Y K+
Sbjct: 121 LILGLAFGIRYYTKT 135

[Close]

BLAST of CSPI02G09060 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 131.0 bits (328), Expect = 7.4e-31
Identity = 61/127 (48.03%), Postives = 88/127 (69.29%), Query Frame = 0


Query: 7   LFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSE 66
           L +  +V++H    DCW++I+GKVY+++ F+++HPGGD VLL+ TGKDA+ DFEDV HS+
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  SARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTT--EFIIKLLQFLVPLAILGLA 126
            A+E M KY +G++D ST+P    Y  P +      +TT  E   KLL +L+PL ILG+A
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

Query: 127 VGIRFYN 132
             +RFYN
Sbjct: 124 FALRFYN 130

[Close]

BLAST of CSPI02G09060 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 127.9 bits (320), Expect = 6.3e-30
Identity = 57/127 (44.88%), Postives = 90/127 (70.87%), Query Frame = 0


Query: 6   KLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 65
           KL+++ E + HN   DCW++I+GKVY+V+ ++++HPGGD+VLL+  GKDATDDFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  ESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQ-----FLVPLAI 125
           + ARE M KY++GE+D S+LPE      P+   Y +D+  + + KL       ++VP++I
Sbjct: 66  KDARELMEKYFIGELDESSLPEI-----PELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAVGI 128
           + ++V +
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:

BLAST of CSPI02G09060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48845	9.7e-60	78.95	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49099	4.1e-58	76.12	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49098	1.5e-57	74.63	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O04354	1.2e-54	74.62	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]
P49100	3.9e-53	75.00	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of CSPI02G09060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHS9	5.1e-72	99.25	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A5D3CSF5	1.3e-70	96.27	Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G0...	[more]
A0A1S3C5R9	1.3e-70	96.27	cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103496974 PE=3 SV=1	[more]
A0A6J1CAX1	8.5e-67	90.30	cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1	[more]
A0A6J1H4S5	1.6e-65	90.30	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111460140 PE=3 SV=1	[more]

BLAST of CSPI02G09060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138957.1	1.1e-71	99.25	cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cuc...	[more]
XP_008457249.1	2.6e-70	96.27	PREDICTED: cytochrome b5-like [Cucumis melo] >KAA0033284.1 cytochrome b5-like [C...	[more]
XP_022138846.1	1.8e-66	90.30	cytochrome b5-like [Momordica charantia]	[more]
XP_022959033.1	3.3e-65	90.30	cytochrome b5-like [Cucurbita moschata]	[more]
XP_023006481.1	4.3e-65	90.30	cytochrome b5-like [Cucurbita maxima]	[more]

BLAST of CSPI02G09060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32720.1	6.9e-61	78.95	cytochrome B5 isoform B	[more]
AT5G53560.1	1.9e-50	66.67	cytochrome B5 isoform E	[more]
AT5G48810.1	7.9e-49	62.96	cytochrome B5 isoform D	[more]
AT2G46650.1	7.4e-31	48.03	cytochrome B5 isoform C	[more]
AT1G26340.1	6.3e-30	44.88	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 40..54 score: 53.54 coord: 30..40 score: 61.02 coord: 68..80 score: 34.97 coord: 55..62 score: 48.11
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 1.6E-34 score: 130.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 9..80 e-value: 3.8E-27 score: 94.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 29.434013
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 3..85 e-value: 7.5E-35 score: 120.8
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 6..89
None	No IPR available	PANTHER	PTHR19359:SF110	BNAC04G43300D PROTEIN	coord: 1..132
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..132
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G09060.1	CSPI02G09060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043447	alkane biosynthetic process
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding