CSPI02G03690 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G03690
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	kunitz trypsin inhibitor 1-like
Location	Chr2: 2588656 .. 2589440 (-)
RNA-Seq Expression	CSPI02G03690
Synteny	CSPI02G03690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTCAATCGACTTTGTGCAATTTTGATTTCCTCCAAAGCTATATCTATAAATAGTCTGCAACAACACCCACATTCTTCATAATCACAAAACACAATCCTTTTTCTAGACATATTAAAATATCAAGTACTTGAAGAATAACAAGATGACGATGCTACATAAATCACTGGCCATCTTTGGCTATGCATGTCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCCGTGCTCGACACCAACGGTCAACCTCTGCAACGCGGTGTCGAGTACTACATCTCACCTGCAATTACTGACGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAACGCTCCGTGCCCGCTCTTCGTTGGCCAGGAACCCGTTACTTCGACAAATATCGGTCTCCCAGTCACCTTTAGACCCACTGAGGAGGGCATAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACATGTGTAACATCAACACAATGGAGAGTGGATGCAACTGAGTCTGATACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGACCTGCTGGGATCTTTGGGATCAGTAGGGACAACGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGATGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGAGATCTAAAATGGAAGAATTTGGTTTAATTAATCTTCTTGCAAGGGAAAAAAATG

mRNA sequence

CCTCAATCGACTTTGTGCAATTTTGATTTCCTCCAAAGCTATATCTATAAATAGTCTGCAACAACACCCACATTCTTCATAATCACAAAACACAATCCTTTTTCTAGACATATTAAAATATCAAGTACTTGAAGAATAACAAGATGACGATGCTACATAAATCACTGGCCATCTTTGGCTATGCATGTCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCCGTGCTCGACACCAACGGTCAACCTCTGCAACGCGGTGTCGAGTACTACATCTCACCTGCAATTACTGACGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAACGCTCCGTGCCCGCTCTTCGTTGGCCAGGAACCCGTTACTTCGACAAATATCGGTCTCCCAGTCACCTTTAGACCCACTGAGGAGGGCATAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACATGTGTAACATCAACACAATGGAGAGTGGATGCAACTGAGTCTGATACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGACCTGCTGGGATCTTTGGGATCAGTAGGGACAACGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGATGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGAGATCTAAAATGGAAGAATTTGGTTTAATTAATCTTCTTGCAAGGGAAAAAAATG

Coding sequence (CDS)

ATGACGATGCTACATAAATCACTGGCCATCTTTGGCTATGCATGTCTCTTCATGGCCATAACTTCCACAGCTCAATCGCCTCCCGTGCTCGACACCAACGGTCAACCTCTGCAACGCGGTGTCGAGTACTACATCTCACCTGCAATTACTGACGTTGGGGGCAATCTCACCCTAAAAAGTCGTAGTAACGCTCCGTGCCCGCTCTTCGTTGGCCAGGAACCCGTTACTTCGACAAATATCGGTCTCCCAGTCACCTTTAGACCCACTGAGGAGGGCATAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACATGTGTAACATCAACACAATGGAGAGTGGATGCAACTGAGTCTGATACCGGAAGAAGGTTCGTGGGGATCGGGGATGAGGACGGACCTGCTGGGATCTTTGGGATCAGTAGGGACAACGGAGCTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGATGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGGTGGCTTTGGATGGTGATGCTTTTCCTTTTGAGTTTATCAAAGCTTGA

Protein sequence

MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA*

Homology

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BLAST of CSPI02G03690 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.9e-27
Identity = 79/205 (38.54%), Postives = 107/205 (52.20%), Query Frame = 0


Query: 1   MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60
           M +  ++L I  + CLF+  T+ AQ   VLDT G+P++   EY+I P IT+ GG  T+ S
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVS 62

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRP--TEEGIDIIDEGTSLNIVFQALSTCVTSTQW 120
           R N  CPL VG E +T    GL V F P       D +     L I FQA S+CV ST+W
Sbjct: 63  R-NESCPLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEW 122

Query: 121 RVDATESDTGRRFVGIGDEDGPAGIFG------ISRDNGAYNIVWCPAMM---GRPRCGR 180
           R+   ++ +GRR +  G +    G +G       +   G YNI WCP  +    +  CG 
Sbjct: 123 RLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGT 182

Query: 181 AGILVENGVRLVALDGDAFPFEFIK 195
             +L ENG  L+ALDG   P  F K
Sbjct: 183 VDMLNENGKILLALDGGPLPLVFQK 203

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BLAST of CSPI02G03690 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.2e-27
Identity = 69/197 (35.03%), Postives = 110/197 (55.84%), Query Frame = 0


Query: 7   SLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPC 66
           S  IF  A +++ + +T+ +  V+DT+G+P++   +Y+I PAIT  GG+LTL +R++  C
Sbjct: 5   SFTIFILANVWLLVVTTSIAQFVIDTSGEPVENDEDYFIRPAITGNGGSLTLVTRNS--C 64

Query: 67  PLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESD 126
           P  VG +P       + ++   +    D +  G  L ++FQA ++C  ST+WR+   ++ 
Sbjct: 65  PFNVGLDPDAPQGFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDAT 124

Query: 127 TGRRFVGIGDEDGPAGIFG------ISRDNGAYNIVWCPAMM---GRPRCGRAGILVENG 186
           TGRRF+  G +D   G +G       +   G +NI WCP  +    +  CG  GI+ ENG
Sbjct: 125 TGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENG 184

Query: 187 VRLVALDGDAFPFEFIK 195
             L+ALDG A P  F K
Sbjct: 185 KILLALDGSALPVAFQK 199

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BLAST of CSPI02G03690 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 9.2e-27
Identity = 78/204 (38.24%), Postives = 109/204 (53.43%), Query Frame = 0


Query: 3   MLHKSLAIF--GYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 62
           M  +SL IF   +  L MA TS AQ   V+DT+G+P++   EY+I PAIT  GG  TL +
Sbjct: 1   MSTRSLTIFILAHVWLLMATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 63  RSNAPCPLFVGQEPVTSTNIGLPVTFRP--TEEGIDIIDEGTSLNIVFQALSTCVTSTQW 122
             N PCPL VG +    T +G+ V F P   +   D +     L + F   ++C  ST W
Sbjct: 61  -GNGPCPLHVGLDNTEGT-LGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 123 RVDATESDTGRRFVGIGDEDG---PAGIFGI--SRDNGAYNIVWCP---AMMGRPRCGRA 182
           R+   ++ +GRR +  G ++G       F I  ++  G YNI WCP       + +CG  
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 183 GILVENGVRLVALDGDAFPFEFIK 195
           G++ ENG  L+ALDGDA P  F K
Sbjct: 181 GVIRENGKNLLALDGDALPVVFQK 200

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BLAST of CSPI02G03690 vs. ExPASy Swiss-Prot
Match: P16347 (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.8e-13
Identity = 54/143 (37.76%), Postives = 71/143 (49.65%), Query Frame = 0


Query: 27  PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 86
           PPV DT+G  L+    YY+ PA    GG LT+       CPLFV QE       GLPV  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQE-ADGQRDGLPVRI 62

Query: 87  RP--TEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGD--EDGPAG 146
            P        II   T + I F+A +TCV ST+W +D +E  +GRR V  G   +  P+G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHID-SELVSGRRHVITGPVRDPSPSG 122

Query: 147 ---IFGISRDNGA----YNIVWC 159
               F I + +GA    Y ++ C
Sbjct: 123 RENAFRIEKYSGAEVHEYKLMAC 143

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BLAST of CSPI02G03690 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.7e-12
Identity = 51/146 (34.93%), Postives = 69/146 (47.26%), Query Frame = 0


Query: 24  AQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLP 83
           A  PPV DT+G  L+    YY+  A    GG LT+       CPLFV Q+P    + G P
Sbjct: 23  ADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHDGFP 82

Query: 84  VTFRP--TEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDEDGPA 143
           V   P        II   T + I F+A +TC+ ST+W +D +E   GRR V  G    P+
Sbjct: 83  VRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHID-SELAAGRRHVITGPVKDPS 142

Query: 144 -----GIFGISRDNGA----YNIVWC 159
                  F I + +GA    Y ++ C
Sbjct: 143 PSGRENAFRIEKYSGAEVHEYKLMSC 166

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BLAST of CSPI02G03690 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 3.9e-105
Identity = 189/195 (96.92%), Postives = 191/195 (97.95%), Query Frame = 0


Query: 1   MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60
           M MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPL+RGVEYYISPAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTFRPTE G DIIDEGTSLNIVF+ALSTC TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 120

Query: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 VALDGDAFPFEFIKA 196
           VALDGDAFPFEFIKA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of CSPI02G03690 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 8.7e-105
Identity = 188/193 (97.41%), Postives = 190/193 (98.45%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62
           MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPL+RGVEYYISPAITDVGGNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122
           NAPCPLFVGQEPVTSTNIGLPVTFRPTE G DIIDEGTSLNIVF+ALSTC TSTQWRVDA
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182
           TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 183 LDGDAFPFEFIKA 196
           LDGDAFPFEFIKA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of CSPI02G03690 vs. ExPASy TrEMBL
Match: A0A0A0LIK2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 7.9e-98
Identity = 177/178 (99.44%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 18  MAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 77
           MAITSTAQ PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 78  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 137
           TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 138 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 196
           DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]

BLAST of CSPI02G03690 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.7e-82
Identity = 147/193 (76.17%), Postives = 166/193 (86.01%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62
           MLH SLAIF +  +FMAITSTAQ PPVLDT+GQPL+RGVEYYI PAITDV GNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122
           NAPCPL+VGQEP+ STNIGLPVTF P   G DII+E    N+VFQA STC+TSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182
            ES TGRRFVG+G+++GP GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFIKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of CSPI02G03690 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.9e-81
Identity = 145/193 (75.13%), Postives = 165/193 (85.49%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62
           MLH SLAIF Y  +F+AITSTAQ PPVLDT+GQPL+RGVEYYI PAIT+V GNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122
           NAPCPL+VGQEPV STNIGLPVTF P   G DII+E    N+ FQA STC+TSTQWRVD 
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182
            ES TGRRFVG+G+++GP GIF I R+NG YNIVWCP M+GRPRCG AGILVE+GVRL+A
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFIKA 196
           LDG+AFPFEF+KA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of CSPI02G03690 vs. NCBI nr
Match: KGN60910.2 (hypothetical protein Csa_023447 [Cucumis sativus])

HSP 1 Score: 397.5 bits (1020), Expect = 6.6e-107
Identity = 193/195 (98.97%), Postives = 193/195 (98.97%), Query Frame = 0


Query: 1   MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60
           M MLHKSLAIFGYACLFMAITSTAQ PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS
Sbjct: 170 MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 229

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV
Sbjct: 230 RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 289

Query: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 290 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 349

Query: 181 VALDGDAFPFEFIKA 196
           VALDGDAFPFEFIKA
Sbjct: 350 VALDGDAFPFEFIKA 364

[Close]

BLAST of CSPI02G03690 vs. NCBI nr
Match: XP_004139192.1 (miraculin [Cucumis sativus])

HSP 1 Score: 397.5 bits (1020), Expect = 6.6e-107
Identity = 193/195 (98.97%), Postives = 193/195 (98.97%), Query Frame = 0


Query: 1   MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60
           M MLHKSLAIFGYACLFMAITSTAQ PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120

Query: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 VALDGDAFPFEFIKA 196
           VALDGDAFPFEFIKA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of CSPI02G03690 vs. NCBI nr
Match: KAA0063690.1 (miraculin-like [Cucumis melo var. makuwa] >TYK18337.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 390.6 bits (1002), Expect = 8.1e-105
Identity = 189/195 (96.92%), Postives = 191/195 (97.95%), Query Frame = 0


Query: 1   MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60
           M MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPL+RGVEYYISPAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTFRPTE G DIIDEGTSLNIVF+ALSTC TSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 120

Query: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 VALDGDAFPFEFIKA 196
           VALDGDAFPFEFIKA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of CSPI02G03690 vs. NCBI nr
Match: KAA0063689.1 (miraculin-like [Cucumis melo var. makuwa] >TYK18338.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 389.4 bits (999), Expect = 1.8e-104
Identity = 188/193 (97.41%), Postives = 190/193 (98.45%), Query Frame = 0


Query: 3   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRS 62
           MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPL+RGVEYYISPAITDVGGNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 63  NAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDA 122
           NAPCPLFVGQEPVTSTNIGLPVTFRPTE G DIIDEGTSLNIVF+ALSTC TSTQWRVDA
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 123 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 182
           TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 183 LDGDAFPFEFIKA 196
           LDGDAFPFEFIKA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of CSPI02G03690 vs. NCBI nr
Match: XP_038888526.1 (kunitz type trypsin inhibitor 106-like [Benincasa hispida])

HSP 1 Score: 350.5 bits (898), Expect = 9.3e-93
Identity = 168/195 (86.15%), Postives = 178/195 (91.28%), Query Frame = 0


Query: 1   MTMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKS 60
           M MLHKSLAIFGYACLFMAITSTAQ PPVLDT+ QPL+RGVEYYI PAITDVGGNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTDSQPLRRGVEYYIKPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRV 120
           RSNAPCPLFVGQEPVTSTNIGLPVTF P   G DII+E  SLNIVF+ALSTCVTSTQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIVAGEDIIEESRSLNIVFEALSTCVTSTQWRV 120

Query: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180
           D  ESDTGRRFVGIG++DGP+GIF I R+NG YNIVWCPAMMGRPRCG AGILVENGVRL
Sbjct: 121 DEAESDTGRRFVGIGNDDGPSGIFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVENGVRL 180

Query: 181 VALDGDAFPFEFIKA 196
           +ALDGDAFPFEF+KA
Sbjct: 181 LALDGDAFPFEFVKA 195

[Close]

BLAST of CSPI02G03690 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 79.0 bits (193), Expect = 4.9e-15
Identity = 60/191 (31.41%), Postives = 94/191 (49.21%), Query Frame = 0


Query: 14  ACLFMAITSTAQSPP--VLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVG 73
           + +F A ++   +P   VLD  G P+Q  V+YYI PA    GG L   +R+ +   L + 
Sbjct: 15  SAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLN 74

Query: 74  QEPVTSTN---IGLPVTFRPTEEGIDIIDEGTSLNIVFQA-LSTCVTSTQWRVDATESDT 133
            + V S++    GLPVTF P    +  +    SLN+ F + +  C  S  WR+D +    
Sbjct: 75  LDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHS-VQL 134

Query: 134 GRRFVGIGDEDGPAGI-FGISRDNGAYNIVWCPAMMGRPRCGRAGILV-ENGVRLVALDG 193
            + FV IG + G     F I  D  AY +++CP +     C    + + ++GVR + L  
Sbjct: 135 RKSFVSIGGQKGKGNSWFQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRRLVLST 194

Query: 194 D-AFPFEFIKA 196
           D +F  +F KA
Sbjct: 195 DQSFVVKFQKA 203

[Close]

BLAST of CSPI02G03690 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.3e-12
Identity = 57/187 (30.48%), Postives = 83/187 (44.39%), Query Frame = 0


Query: 8   LAIFGYACLFMA---ITSTAQSPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNA 67
           L IF    +F++   +T+ A   PV D NG+ L  GV YYI P I   GG LT+ +    
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 68  PCPLFVGQEPVTSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATE 127
            CP  V Q+     + GLPV F P ++    I   T +NI F   S       W +   +
Sbjct: 65  TCPTSVIQDQF-EVSQGLPVKFSPYDKS-RTIPVSTDVNIKFSPTSI------WELANFD 124

Query: 128 SDTGRRFVGIGDEDGPAG------IFGISRDNGAYNIVWCPAMMG--RPRCGRAGILVEN 184
             T + F+     +G  G       F I +    Y I +CP +    +  C   G+ V++
Sbjct: 125 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

The following BLAST results are available for this feature:

BLAST of CSPI02G03690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV1	1.9e-27	38.54	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
G7LCV7	3.2e-27	35.03	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
A0A072TH68	9.2e-27	38.24	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
P16347	5.8e-13	37.76	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 ...	[more]
P07596	1.7e-12	34.93	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of CSPI02G03690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D468	3.9e-105	96.92	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A5A7VDV8	8.7e-105	97.41	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A0A0LIK2	7.9e-98	99.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1	[more]
A0A6J1G3G3	2.7e-82	76.17	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	3.9e-81	75.13	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]

BLAST of CSPI02G03690 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN60910.2	6.6e-107	98.97	hypothetical protein Csa_023447 [Cucumis sativus]	[more]
XP_004139192.1	6.6e-107	98.97	miraculin [Cucumis sativus]	[more]
KAA0063690.1	8.1e-105	96.92	miraculin-like [Cucumis melo var. makuwa] >TYK18337.1 miraculin-like [Cucumis me...	[more]
KAA0063689.1	1.8e-104	97.41	miraculin-like [Cucumis melo var. makuwa] >TYK18338.1 miraculin-like [Cucumis me...	[more]
XP_038888526.1	9.3e-93	86.15	kunitz type trypsin inhibitor 106-like [Benincasa hispida]	[more]

BLAST of CSPI02G03690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73325.1	4.9e-15	31.41	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G17860.1	1.3e-12	30.48	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 28..195 e-value: 9.6E-30 score: 114.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 29..194 e-value: 4.8E-40 score: 137.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 2..194
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 29..45
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 28..194 e-value: 2.94268E-40 score: 132.851
None	No IPR available	GENE3D	2.80.10.50		coord: 23..195 e-value: 1.6E-46 score: 160.1
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 2..194
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G03690.1	CSPI02G03690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0003953	NAD+ nucleosidase activity