CSPI02G03680 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G03680
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	kunitz trypsin inhibitor 1-like
Location	Chr2: 2582399 .. 2583631 (-)
RNA-Seq Expression	CSPI02G03680
Synteny	CSPI02G03680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTAAATGAAGAAATAGTAATTATTGTGATAAAGCTAGACTTTGGTCATTTTCATATCCTCCATGTTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATCATTTTTCAAACAAAACTAAATCACTCTTGTTGAACAATCAAAAGATGATGAGGTTCCATAAGTCACTAATTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTATGTATTAAATCTATTACTAAACTAAATTGATTCAATAAGTTATGTGATATCTGTTAGAGTGTTGATATACAACTATTAAATGTGTACCTACACTCACTCACTATTTAAGCAGATAGATTATGATATATTGAATTCTTAACAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAATGTGTAAATTTGTTGTAATTGTATACACACTCAATTAAAACTAAATAAAACTTGATGTAAGGATTTACTTTTATGTGTGTTCATTGGGGTGTTATATATGATTAATATATATAATGTGATTTGGTAGGTGGATTGGTGTTAAGTAAAAAAGAAAATTAGAGAAATAACAAGTAGAGAAAATAAATGAAATTGTCAACAAATGCTCCTAAAAATCATATAATTGATAGAGACAAATAGCAAATAGTCAAGCTTACCTCCAATGAGAGAAAATTTGTCATAGG

mRNA sequence

AGTAAATGAAGAAATAGTAATTATTGTGATAAAGCTAGACTTTGGTCATTTTCATATCCTCCATGTTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATCATTTTTCAAACAAAACTAAATCACTCTTGTTGAACAATCAAAAGATGATGAGGTTCCATAAGTCACTAATTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAATGTGTAAATTTGTTGTAATTGTATACACACTCAATTAAAACTAAATAAAACTTGATGTAAGGATTTACTTTTATGTGTGTTCATTGGGGTGTTATATATGATTAATATATATAATGTGATTTGGTAGGTGGATTGGTGTTAAGTAAAAAAGAAAATTAGAGAAATAACAAGTAGAGAAAATAAATGAAATTGTCAACAAATGCTCCTAAAAATCATATAATTGATAGAGACAAATAGCAAATAGTCAAGCTTACCTCCAATGAGAGAAAATTTGTCATAGG

Coding sequence (CDS)

ATGATGAGGTTCCATAAGTCACTAATTGCCCTCTTTAGCTGGCTATGCTTCTTCATGGCCATACCCTCTACTGCCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGTCGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGCGATCAGTGCCCATTTTACGTTGGGCAAGTACCACTTCTTTCACAAGAAATAGGTTTCGGGGTCACCCTCACACCTTACCTAGAGGGTGACGATACAATCAGAGAAGGCAGAGATTTGAAGTTCGTGTTTCAAGCGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGAAGATCCAAAGAGCGGAAGGAGGTTTGTGAAGCTCGGAAACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCAACCTCGGAGTTTATAATATAGGATGGTGTCCCTCAGATGTGCCCTTTAAAGGGAGGCCAAGGTGTGGAAGTGCAGGAATTTTGATTGAGAAAGGTGTGAGGTTTTTGGCTTTGGATGGACCAGCATTTCCTTTTGAGTTTGTGAGAGTTGATACTGGAGAAGAGCTTGGTTCTATCCTGAAAGAGAAAAAATAA

Protein sequence

MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK*

Homology

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BLAST of CSPI02G03680 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.9e-37
Identity = 84/193 (43.52%), Postives = 118/193 (61.14%), Query Frame = 0


Query: 14  WLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYV 73
           WL   +   S AQF    V+DT G P++ D +YFI+PAIT   G+LTL+TR  + CPF V
Sbjct: 15  WL--LVVTTSIAQF----VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFNV 74

Query: 74  GQVPLLSQEIGFGVTLTPYLEG--DDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSG 133
           G  P   Q  GF V L+P++    +D +R GRDL+ +FQA T C   T+W++GE D  +G
Sbjct: 75  GLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTG 134

Query: 134 RRFVKLGNDNTATG----YFRIDKS-NLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVR 193
           RRF+  G D++  G    +FRI ++ + G++NI WCP++V    +  CG+ GI+ E G  
Sbjct: 135 RRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKI 194

Query: 194 FLALDGPAFPFEF 200
            LALDG A P  F
Sbjct: 195 LLALDGSALPVAF 197

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BLAST of CSPI02G03680 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.9e-34
Identity = 79/205 (38.54%), Postives = 115/205 (56.10%), Query Frame = 0


Query: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61
           MR     + + + +C F+   + AQF    VLDT G P++ D EYFI+P IT+  G  T+
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRSTM 62

Query: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYL--EGDDTIREGRDLKFVFQAFTICITGT 121
           ++R  + CP +VG + L     G  V  TP+      D +R  RDL+  FQA + C+  T
Sbjct: 63  VSR-NESCPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQST 122

Query: 122 QWKVGEEDPKSGRRFVKLGNDNTATG----YFRIDKSNL-GVYNIGWCPSDVPFKGRPRC 181
           +W++GE+D KSGRR +  G D+   G    +FRI ++ L G+YNI WCP++V    +  C
Sbjct: 123 EWRLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFEC 182

Query: 182 GSAGILIEKGVRFLALDGPAFPFEF 200
           G+  +L E G   LALDG   P  F
Sbjct: 183 GTVDMLNENGKILLALDGGPLPLVF 201

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BLAST of CSPI02G03680 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 3.3e-33
Identity = 79/197 (40.10%), Postives = 107/197 (54.31%), Query Frame = 0


Query: 9   IALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQ 68
           I + + +   MA  S AQF    V+DT G P++ D EYFI+PAIT   G  TL+T  G  
Sbjct: 8   IFILAHVWLLMATTSIAQF----VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG-P 67

Query: 69  CPFYVGQVPLLSQEIGFGVTLTPYL--EGDDTIREGRDLKFVFQAFTICITGTQWKVGEE 128
           CP +VG +      +G  V  TP+     DD +R  RDL+  F   T C   T W++GE+
Sbjct: 68  CPLHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEK 127

Query: 129 DPKSGRRFVKLGNDNTA---TGYFRIDKSNL-GVYNIGWCPSDVPFKGRPRCGSAGILIE 188
           D  SGRR +  G DN A     +FRI ++   G YNI WCP++     + +CG+ G++ E
Sbjct: 128 DATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRE 187

Query: 189 KGVRFLALDGPAFPFEF 200
            G   LALDG A P  F
Sbjct: 188 NGKNLLALDGDALPVVF 198

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BLAST of CSPI02G03680 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.0e-15
Identity = 56/183 (30.60%), Postives = 81/183 (44.26%), Query Frame = 0


Query: 32  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTP 91
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 92  YLEGDDTIREGRDLKFVFQ-AFTICITGTQWKVGEEDPKSGRRFVKLG------NDNTAT 151
           +      + E ++L        TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 152 GYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 207
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

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BLAST of CSPI02G03680 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 7.6e-14
Identity = 57/171 (33.33%), Postives = 76/171 (44.44%), Query Frame = 0


Query: 15  LCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVG 74
           L   +AI  +     PPV DTEG  L  D  Y++ PA     G LT+  R    CP  V 
Sbjct: 10  LLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV-LPCPLLVA 69

Query: 75  QVPLLSQEIGFGVTLTPY----LEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKS 134
           Q     +  GF V  TP+       D TIR   D++  F A TIC+  T+W VG+E P +
Sbjct: 70  Q-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDE-PLT 129

Query: 135 GRRFVKLG-----NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGS 177
           G R V  G     + +     FR++K   G Y +  C       G  R G+
Sbjct: 130 GARRVVTGPLIGPSPSGRENAFRVEKYG-GGYKLVSCRDSCQDLGVSRDGA 176

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BLAST of CSPI02G03680 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 4.5e-126
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120
           LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180
           WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 217
           IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of CSPI02G03680 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.4e-113
Identity = 197/217 (90.78%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MMRFHKSL A+FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 120
           TLITRKGDQCPFYVGQVPL SQE GFGV+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 180
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 217
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEKK 217

[Close]

BLAST of CSPI02G03680 vs. ExPASy TrEMBL
Match: A0A1S3C0M7 (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.1e-111
Identity = 195/216 (90.28%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MMRFHKSL ++FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 120
           TLITRKGDQCPFYVGQVPLLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 180
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEK 216
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of CSPI02G03680 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.1e-87
Identity = 156/200 (78.00%), Postives = 173/200 (86.50%), Query Frame = 0


Query: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61
           M   KSL+A FS+LC  MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL
Sbjct: 1   MMLCKSLVA-FSYLCLVMAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTL 60

Query: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121
           +TRKGDQCPF+VGQVPL SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W
Sbjct: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAW 120

Query: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181
           +VGE DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 121 RVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180

Query: 182 EKGVRFLALDGPAFPFEFVR 202
           EKG+R LALDG AFPFEFVR
Sbjct: 181 EKGLRLLALDGSAFPFEFVR 197

[Close]

BLAST of CSPI02G03680 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 6.5e-85
Identity = 153/200 (76.50%), Postives = 169/200 (84.50%), Query Frame = 0


Query: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61
           M   KSL+A FS+LC  MA  S AQ   PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL
Sbjct: 1   MMLCKSLVA-FSYLCLVMAASSIAQ--SPPVLDIEGRPLEINVEYFIKPAITDVAGNLTL 60

Query: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121
           +TRKGDQCPF+VGQVPL SQEIGFGVT TPYL  +D IREGRDLK VFQAF+ICIT T W
Sbjct: 61  LTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAW 120

Query: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181
           +VGE DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 121 RVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 180

Query: 182 EKGVRFLALDGPAFPFEFVR 202
           EKG+R LALDG AFPFEFVR
Sbjct: 181 EKGLRLLALDGSAFPFEFVR 197

[Close]

BLAST of CSPI02G03680 vs. NCBI nr
Match: XP_004139193.1 (endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus])

HSP 1 Score: 460.3 bits (1183), Expect = 9.2e-126
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120
           LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180
           WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 217
           IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of CSPI02G03680 vs. NCBI nr
Match: KAE8651587.1 (hypothetical protein Csa_023465 [Cucumis sativus])

HSP 1 Score: 423.7 bits (1088), Expect = 9.6e-115
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0


Query: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78
           MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL
Sbjct: 1   MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 60

Query: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138
           LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG
Sbjct: 61  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 120

Query: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198
           NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE
Sbjct: 121 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 180

Query: 199 FVRVDTGEELGSILKEKK 217
           FVRVDTGEELGSILKEKK
Sbjct: 181 FVRVDTGEELGSILKEKK 198

[Close]

BLAST of CSPI02G03680 vs. NCBI nr
Match: KAA0063688.1 (endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >TYK18339.1 endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 197/217 (90.78%), Postives = 203/217 (93.55%), Query Frame = 0


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MMRFHKSL A+FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 120
           TLITRKGDQCPFYVGQVPL SQE GFGV+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 180
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 217
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEKK 217

[Close]

BLAST of CSPI02G03680 vs. NCBI nr
Match: XP_008455014.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo])

HSP 1 Score: 412.5 bits (1059), Expect = 2.2e-111
Identity = 195/216 (90.28%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MMRFHKSL ++FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 120
           TLITRKGDQCPFYVGQVPLLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 180
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEK 216
           LIEKGVRFLALDGPAFPFEFVRVD  EELGSIL+EK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of CSPI02G03680 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 391.7 bits (1005), Expect = 4.0e-105
Identity = 187/217 (86.18%), Postives = 199/217 (91.71%), Query Frame = 0


Query: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MM FHKSL A+FS LCFFMAI S AQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMMFHKSL-AIFSSLCFFMAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120
           LITRKGDQCPFYVGQVPLLSQ+IGFGVT TPYL G+DTIREGRDLK VFQAFTICIT TQ
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQ 120

Query: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180
           WKVGE DP +GRRFVKLGND+TATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGIL
Sbjct: 121 WKVGEADPITGRRFVKLGNDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180

Query: 181 IEKGVRFLALDGPAFPFEFVRVD-TGEELGSILKEKK 217
           IEKG+R LALDG AFPFEFVRV+  G++L SI +EKK
Sbjct: 181 IEKGLRLLALDGSAFPFEFVRVEIAGKKLDSIFQEKK 216

[Close]

BLAST of CSPI02G03680 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 83.2 bits (204), Expect = 2.9e-16
Identity = 56/183 (30.60%), Postives = 81/183 (44.26%), Query Frame = 0


Query: 32  VLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQEIGFGVTLTP 91
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 92  YLEGDDTIREGRDLKFVFQ-AFTICITGTQWKVGEEDPKSGRRFVKLG------NDNTAT 151
           +      + E ++L        TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 152 GYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI-EKGVRFLALDGPAFPFEFVRVD 207
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

The following BLAST results are available for this feature:

BLAST of CSPI02G03680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	2.9e-37	43.52	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	3.9e-34	38.54	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	3.3e-33	40.10	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
Q8RXD5	4.0e-15	30.60	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P29421	7.6e-14	33.33	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of CSPI02G03680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGB4	4.5e-126	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A5A7V7Y2	7.4e-113	90.78	Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C0M7	1.1e-111	90.28	endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1FD70	1.1e-87	78.00	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLP6	6.5e-85	76.50	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]

BLAST of CSPI02G03680 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139193.1	9.2e-126	100.00	endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus]	[more]
KAE8651587.1	9.6e-115	100.00	hypothetical protein Csa_023465 [Cucumis sativus]	[more]
KAA0063688.1	1.5e-112	90.78	endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >T...	[more]
XP_008455014.1	2.2e-111	90.28	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo]	[more]
XP_038888505.1	4.0e-105	86.18	kunitz type trypsin inhibitor 111-like [Benincasa hispida]	[more]

BLAST of CSPI02G03680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73260.1	2.9e-16	30.60	kunitz trypsin inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 31..203 e-value: 8.2E-27 score: 105.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..200 e-value: 9.7E-36 score: 123.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..202
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48
None	No IPR available	GENE3D	2.80.10.50		coord: 29..204 e-value: 3.9E-38 score: 132.9
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 8..202
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 29..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G03680.1	CSPI02G03680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity