CSPI02G02630 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G02630
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr2: 1817334 .. 1818617 (+)
RNA-Seq Expression	CSPI02G02630
Synteny	CSPI02G02630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGATCACTTGTGAGTGCCCAAAATACTAATTGGGAAGGTGTAGTGAGCAGGTCGATCTACATTTATTTCTCTCACTTCCTTATTTTATAAAAATTGTATAGTTTTCATTGATATATATTACTTATTTGTTAATATTCAATAATCTCATGTGTTCGTGATCACAGCCGTCGCACCAGTTCAGGTAGGTTTCTTGCTAAAGGGCAGAACCAACTCGTCCGTAGAATAGAGAAAAGAATAGCAGAATTTACATTCATTCCCGTAGGTAATGAAAAACAAAATAATTAAAATTCTAAACTTAATTCATAATAACGTCAACCCAAACATACATTTTAGTATTGATCATATGTTCACAAATTTCAAACAGAAAATGGAGAAGGATTGAGTATTCTACATTATGAAGTTGGGCAGAAGTTTGAACCTCATCATGATTACACTCATCCTGATTCATTCAGCTTTAAAAGTTTGGGCCAAAAAATGCCACCCTCGTCATGTATCTGTAAGATTCTACTCACCCCTAATCTGATTGAAATGTAACACAATAATCCAATAATGCATGTTGAATTGTAACACATTAAAATCGTTTCAGTTTCCTTGGTACTGATACGAATGCAGGTCGGGTGTCAAAGAAGGGGTTGCGACGGTATTCCCGGAGGCGAAAAAATGCGCCAGCTCTGCACGACGATGGTGGAAGAAACTGCCTGAATATGGTAAAGATAATGGACTCTCCGTAAAACCAAAGATGGGAGATGCTTTATTGTTTTGGAGCGTGAAACCTGATGGTACATTGGATCCTACAAGTTTGCATGGTTAGTGATCTTTACTTCACCAAAATCTTTTATATGTATAATTCCATCTGTTTTATTATTATTATTTACATATGATGAGAGTTACAAAATCAGAATACTGTCATAAGGTATGGAAGAAATTATGAATCTGAAATCCATCTTTGTATGGTTGTTTTTGGTAAAAGAACAAAAATATTGTTTTGTGGCTTATTTATTTTTGAGTGTACGTTCATCACAACTTCTGATATATATATATGTATATTGGTGGTATATAGCTTCTTCTCCAGTTGTAAAGGGAGACAAATGGGTTGGTGTAAAGCTGATGCATGTTAAAGCTAAAGATTTAACTCAAGAAGTTATGTATATATTTATAGTTCTTAATTGTATTTAGATTAAATTTTCGTTTTATTTTGTGTGATATTTGATCTGATTTTAAGAGGGATTATTTTGAAATTGCAGGGCTAATAGATGCAGAAGTACATCTAATAGATGA

mRNA sequence

ATGGAGAGATCACTTGTGAGTGCCCAAAATACTAATTGGGAAGGTGTAGTGAGCAGCCGTCGCACCAGTTCAGGTAGGTTTCTTGCTAAAGGGCAGAACCAACTCGTCCGTAGAATAGAGAAAAGAATAGCAGAATTTACATTCATTCCCGTAGAAAATGGAGAAGGATTGAGTATTCTACATTATGAAGTTGGGCAGAAGTTTGAACCTCATCATGATTACACTCATCCTGATTCATTCAGCTTTAAAAGTTTGGGCCAAAAAATGCCACCCTCGTCATGTATCTGTAAGATTCTACTCACCCCTAATCTGATTGAAATGTCGGGTGTCAAAGAAGGGGTTGCGACGGTATTCCCGGAGGCGAAAAAATGCGCCAGCTCTGCACGACGATGGTGGAAGAAACTGCCTGAATATGGTAAAGATAATGGACTCTCCGTAAAACCAAAGATGGGAGATGCTTTATTGTTTTGGAGCGTGAAACCTGATGGTACATTGGATCCTACAAGTTTGCATGCTTCTTCTCCAGTTGTAAAGGGAGACAAATGGGTTGGTGTAAAGCTGATGCATGTTAAAGCTAAAGATTTAACTCAAGAAGTTATGGCTAATAGATGCAGAAGTACATCTAATAGATGA

Coding sequence (CDS)

ATGGAGAGATCACTTGTGAGTGCCCAAAATACTAATTGGGAAGGTGTAGTGAGCAGCCGTCGCACCAGTTCAGGTAGGTTTCTTGCTAAAGGGCAGAACCAACTCGTCCGTAGAATAGAGAAAAGAATAGCAGAATTTACATTCATTCCCGTAGAAAATGGAGAAGGATTGAGTATTCTACATTATGAAGTTGGGCAGAAGTTTGAACCTCATCATGATTACACTCATCCTGATTCATTCAGCTTTAAAAGTTTGGGCCAAAAAATGCCACCCTCGTCATGTATCTGTAAGATTCTACTCACCCCTAATCTGATTGAAATGTCGGGTGTCAAAGAAGGGGTTGCGACGGTATTCCCGGAGGCGAAAAAATGCGCCAGCTCTGCACGACGATGGTGGAAGAAACTGCCTGAATATGGTAAAGATAATGGACTCTCCGTAAAACCAAAGATGGGAGATGCTTTATTGTTTTGGAGCGTGAAACCTGATGGTACATTGGATCCTACAAGTTTGCATGCTTCTTCTCCAGTTGTAAAGGGAGACAAATGGGTTGGTGTAAAGCTGATGCATGTTAAAGCTAAAGATTTAACTCAAGAAGTTATGGCTAATAGATGCAGAAGTACATCTAATAGATGA

Protein sequence

MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPEAKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGDKWVGVKLMHVKAKDLTQEVMANRCRSTSNR*

Homology

[Close]

BLAST of CSPI02G02630 vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.3e-44
Identity = 98/190 (51.58%), Postives = 128/190 (67.37%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           ME+S V  + T  +   S  RTSSG FLA+G+++ +R IEKRI++FTFIPVE+GEGL +L
Sbjct: 110 MEKSTVVDEKTG-KSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVL 169

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYE+GQK+EPH+DY   D ++ ++ GQ+           +   L+ +S V+EG  TVFP 
Sbjct: 170 HYEIGQKYEPHYDY-FMDEYNTRNGGQR-----------IATVLMYLSDVEEGGETVFPA 229

Query: 121 AKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGD 180
           AK    SA  WW +L E GK  GLSVKPKMGDALLFWS+ PD TLDP+SLH    V+KG+
Sbjct: 230 AKG-NYSAVPWWNELSECGK-GGLSVKPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGN 284

Query: 181 KWVGVKLMHV 191
           KW   K + V
Sbjct: 290 KWSSTKWLRV 284

[Close]

BLAST of CSPI02G02630 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.0e-44
Identity = 91/170 (53.53%), Postives = 119/170 (70.00%), Query Frame = 0


Query: 21  RTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYTHPDSF 80
           RTSSG FL +G++++++ IEKRIA++TFIP ++GEGL +LHYE GQK+EPH+DY   D F
Sbjct: 127 RTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDY-FVDEF 186

Query: 81  SFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPEAKKCASSARRWWKKLPEYGK 140
           + K+ GQ+M              L+ +S V+EG  TVFP A    SS   W+ +L E GK
Sbjct: 187 NTKNGGQRM-----------ATMLMYLSDVEEGGETVFPAANMNFSSV-PWYNELSECGK 246

Query: 141 DNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGDKWVGVKLMHV 191
             GLSVKP+MGDALLFWS++PD TLDPTSLH   PV++G+KW   K MHV
Sbjct: 247 -KGLSVKPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHV 282

[Close]

BLAST of CSPI02G02630 vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 4.9e-42
Identity = 90/177 (50.85%), Postives = 115/177 (64.97%), Query Frame = 0


Query: 14  EGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHD 73
           + + S  RTSSG FL +G +++V  IE RI++FTFIP ENGEGL +LHYEVGQ++EPHHD
Sbjct: 124 KSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHHD 183

Query: 74  YTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPEAKKCASSARRWWK 133
           Y   D F+ +  GQ+           +   L+ +S V EG  TVFP AK   S    WW 
Sbjct: 184 YFF-DEFNVRKGGQR-----------IATVLMYLSDVDEGGETVFPAAKGNVSDV-PWWD 243

Query: 134 KLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGDKWVGVKLMHV 191
           +L + GK+ GLSV PK  DALLFWS+KPD +LDP+SLH   PV+KG+KW   K  HV
Sbjct: 244 ELSQCGKE-GLSVLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHV 286

[Close]

BLAST of CSPI02G02630 vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 5.4e-41
Identity = 96/191 (50.26%), Postives = 125/191 (65.45%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSR-RTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSI 60
           M +S V  + T   G   SR RTSSG FL +G +++V  IEKRI++FTFIPVENGEGL +
Sbjct: 112 MVKSTVVDEKTG--GSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQV 171

Query: 61  LHYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFP 120
           LHY+VGQK+EPH+DY   D F+ K+ GQ+           +   L+ +S V +G  TVFP
Sbjct: 172 LHYQVGQKYEPHYDY-FLDEFNTKNGGQR-----------IATVLMYLSDVDDGGETVFP 231

Query: 121 EAKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKG 180
            A+    SA  WW +L + GK+ GLSV PK  DALLFW+++PD +LDP+SLH   PVVKG
Sbjct: 232 AARG-NISAVPWWNELSKCGKE-GLSVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKG 286

Query: 181 DKWVGVKLMHV 191
           +KW   K  HV
Sbjct: 292 NKWSSTKWFHV 286

[Close]

BLAST of CSPI02G02630 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-32
Identity = 80/193 (41.45%), Postives = 117/193 (60.62%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           +E+S+V A N + E V S  RTSSG FL+K Q+ +V  +E ++A +TF+P ENGE + IL
Sbjct: 88  LEKSMV-ADNDSGESVESEVRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQIL 147

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYE GQK+EPH DY H D  + +  G +           +   L+ +S V++G  TVFP 
Sbjct: 148 HYENGQKYEPHFDYFH-DQANLELGGHR-----------IATVLMYLSNVEKGGETVFPM 207

Query: 121 AKKCASSAR-RWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKG 180
            K  A+  +   W +  +     G +VKP+ GDALLF+++ P+ T D  SLH S PVV+G
Sbjct: 208 WKGKATQLKDDSWTECAK----QGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEG 263

Query: 181 DKWVGVKLMHVKA 193
           +KW   + +HVK+
Sbjct: 268 EKWSATRWIHVKS 263

[Close]

BLAST of CSPI02G02630 vs. ExPASy TrEMBL
Match: A0A0A0LIA3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009610 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.6e-102
Identity = 190/210 (90.48%), Postives = 192/210 (91.43%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL
Sbjct: 37  MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 96

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYEVGQKFEPHHDYTHPDSFSFKSLGQ+                + MSGVKEG ATVFPE
Sbjct: 97  HYEVGQKFEPHHDYTHPDSFSFKSLGQRNA-------------TLVMSGVKEGGATVFPE 156

Query: 121 AKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGD 180
           AKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGD
Sbjct: 157 AKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGD 216

Query: 181 KWVGVKLMHVKAKDLTQEVMANRCRSTSNR 211
           KWVGVKLMHVKAKDLTQEVMANRCRSTSNR
Sbjct: 217 KWVGVKLMHVKAKDLTQEVMANRCRSTSNR 233

[Close]

BLAST of CSPI02G02630 vs. ExPASy TrEMBL
Match: A0A5A7V5R0 (Methyltransferase-like protein 6 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329G00820 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 9.5e-73
Identity = 146/203 (71.92%), Postives = 167/203 (82.27%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           M+RSLVS Q  +W+G+VSS RTS+GRFL KGQNQLVRRIEKRIAEFTFIPVENGEGLSIL
Sbjct: 560 MKRSLVSDQK-SWKGLVSSHRTSTGRFLVKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 619

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYEVGQKF+PH+DY+ P+SF+FK+LGQ+           +   ++ +S VKEG ATVFP 
Sbjct: 620 HYEVGQKFDPHYDYSRPESFNFKTLGQR-----------IATLVMYLSDVKEGGATVFPA 679

Query: 121 AKK------CASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASS 180
           AKK      CASS+RRWWKKLPEYG + GLSVKPKMGDALLFWS+KPD TLDPTSLH SS
Sbjct: 680 AKKCPVSKQCASSSRRWWKKLPEYGGE-GLSVKPKMGDALLFWSLKPDSTLDPTSLHGSS 739

Query: 181 PVVKGDKWVGVKLMHVKAKDLTQ 198
           PV++GDKWVGVKLMHV  KDLTQ
Sbjct: 740 PVIEGDKWVGVKLMHV--KDLTQ 747

[Close]

BLAST of CSPI02G02630 vs. ExPASy TrEMBL
Match: A0A1S3CEJ0 (probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103499590 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 4.7e-72
Identity = 145/203 (71.43%), Postives = 166/203 (81.77%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           M+RSLVS Q  +W+G+VSS RTS+GRFL KGQNQLVRRIEKRIAEFTFIPVENGEGLSIL
Sbjct: 46  MKRSLVSDQK-SWKGLVSSHRTSTGRFLVKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 105

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYEVGQKF+PH+DY+ P+SF+FK+LGQ+           +   ++ +S VKEG A VFP 
Sbjct: 106 HYEVGQKFDPHYDYSRPESFNFKTLGQR-----------IATLVMYLSDVKEGGAKVFPA 165

Query: 121 AKK------CASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASS 180
           AKK      CASS+RRWWKKLPEYG + GLSVKPKMGDALLFWS+KPD TLDPTSLH SS
Sbjct: 166 AKKCPVSKQCASSSRRWWKKLPEYGGE-GLSVKPKMGDALLFWSLKPDSTLDPTSLHGSS 225

Query: 181 PVVKGDKWVGVKLMHVKAKDLTQ 198
           PV++GDKWVGVKLMHV  KDLTQ
Sbjct: 226 PVIEGDKWVGVKLMHV--KDLTQ 233

[Close]

BLAST of CSPI02G02630 vs. ExPASy TrEMBL
Match: A0A5D3BDG5 (Putative prolyl 4-hydroxylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001160 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.7e-69
Identity = 143/203 (70.44%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           M+RSLVS Q  +W+G+VSS RTS+GRFL KGQNQLVRRIEKRIAEFTFIPVENGEGLSIL
Sbjct: 256 MKRSLVSDQK-SWKGLVSSHRTSTGRFLVKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 315

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYEVGQKF+PH+DY+ P+SF+FK+LGQ+           +   ++ +S VKEG ATVFP 
Sbjct: 316 HYEVGQKFDPHYDYSRPESFNFKTLGQR-----------IATLVMYLSDVKEGGATVFPA 375

Query: 121 AKK------CASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASS 180
           AKK      CASS+RRWWKKLPEYG + GLSVKPKMGDALLFWS+KPD TLDPTSLH   
Sbjct: 376 AKKCPVSKQCASSSRRWWKKLPEYGGE-GLSVKPKMGDALLFWSLKPDSTLDPTSLH--- 435

Query: 181 PVVKGDKWVGVKLMHVKAKDLTQ 198
            V++GDKWVGVKLMHV  KDLTQ
Sbjct: 436 -VIEGDKWVGVKLMHV--KDLTQ 439

[Close]

BLAST of CSPI02G02630 vs. ExPASy TrEMBL
Match: A0A151S4M6 (Prolyl 4-hydroxylase subunit alpha-1 OS=Cajanus cajan OX=3821 GN=KK1_028532 PE=4 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 5.8e-46
Identity = 102/170 (60.00%), Postives = 123/170 (72.35%), Query Frame = 0


Query: 21  RTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYTHPDSF 80
           RTSSG FLA+G++Q+VR IEKRIA+FTFIPVENGEGL +LHYEVGQK+EPH DY   D+F
Sbjct: 133 RTSSGTFLARGRDQVVRNIEKRIADFTFIPVENGEGLQVLHYEVGQKYEPHFDY-FMDAF 192

Query: 81  SFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPEAKKCASSARRWWKKLPEYGK 140
           + K+ GQ+           +   L+ +S V+EG  TVFP+AK   SS   WW +L E GK
Sbjct: 193 NTKNGGQR-----------IATMLMYLSDVEEGGETVFPDAKGNFSSV-PWWNELSECGK 252

Query: 141 DNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGDKWVGVKLMHV 191
             GLS+KPKMGDALLFWSVKPD TLDP+SLH   PV+KG+KW   K M V
Sbjct: 253 -KGLSIKPKMGDALLFWSVKPDATLDPSSLHGGCPVIKGNKWSCTKWMRV 288

[Close]

BLAST of CSPI02G02630 vs. NCBI nr
Match: XP_031736349.1 (probable prolyl 4-hydroxylase 10 [Cucumis sativus])

HSP 1 Score: 360.1 bits (923), Expect = 1.3e-95
Identity = 179/200 (89.50%), Postives = 183/200 (91.50%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL
Sbjct: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYEVGQKFEPHHDYTHPDSFSFKSLGQ+               ++ +SGVKEG ATVFPE
Sbjct: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQRN-----------ATLVMYLSGVKEGGATVFPE 120

Query: 121 AKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGD 180
           AKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGD
Sbjct: 121 AKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGD 180

Query: 181 KWVGVKLMHVKAKDLTQEVM 201
           KWVGVKLMHVKAKDLTQEVM
Sbjct: 181 KWVGVKLMHVKAKDLTQEVM 189

[Close]

BLAST of CSPI02G02630 vs. NCBI nr
Match: KAA0063572.1 (methyltransferase-like protein 6 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 283.1 bits (723), Expect = 2.0e-72
Identity = 146/203 (71.92%), Postives = 167/203 (82.27%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           M+RSLVS Q  +W+G+VSS RTS+GRFL KGQNQLVRRIEKRIAEFTFIPVENGEGLSIL
Sbjct: 560 MKRSLVSDQK-SWKGLVSSHRTSTGRFLVKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 619

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYEVGQKF+PH+DY+ P+SF+FK+LGQ+           +   ++ +S VKEG ATVFP 
Sbjct: 620 HYEVGQKFDPHYDYSRPESFNFKTLGQR-----------IATLVMYLSDVKEGGATVFPA 679

Query: 121 AKK------CASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASS 180
           AKK      CASS+RRWWKKLPEYG + GLSVKPKMGDALLFWS+KPD TLDPTSLH SS
Sbjct: 680 AKKCPVSKQCASSSRRWWKKLPEYGGE-GLSVKPKMGDALLFWSLKPDSTLDPTSLHGSS 739

Query: 181 PVVKGDKWVGVKLMHVKAKDLTQ 198
           PV++GDKWVGVKLMHV  KDLTQ
Sbjct: 740 PVIEGDKWVGVKLMHV--KDLTQ 747

[Close]

BLAST of CSPI02G02630 vs. NCBI nr
Match: XP_008460837.1 (PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis melo])

HSP 1 Score: 280.8 bits (717), Expect = 9.7e-72
Identity = 145/203 (71.43%), Postives = 166/203 (81.77%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           M+RSLVS Q  +W+G+VSS RTS+GRFL KGQNQLVRRIEKRIAEFTFIPVENGEGLSIL
Sbjct: 46  MKRSLVSDQK-SWKGLVSSHRTSTGRFLVKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 105

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYEVGQKF+PH+DY+ P+SF+FK+LGQ+           +   ++ +S VKEG A VFP 
Sbjct: 106 HYEVGQKFDPHYDYSRPESFNFKTLGQR-----------IATLVMYLSDVKEGGAKVFPA 165

Query: 121 AKK------CASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASS 180
           AKK      CASS+RRWWKKLPEYG + GLSVKPKMGDALLFWS+KPD TLDPTSLH SS
Sbjct: 166 AKKCPVSKQCASSSRRWWKKLPEYGGE-GLSVKPKMGDALLFWSLKPDSTLDPTSLHGSS 225

Query: 181 PVVKGDKWVGVKLMHVKAKDLTQ 198
           PV++GDKWVGVKLMHV  KDLTQ
Sbjct: 226 PVIEGDKWVGVKLMHV--KDLTQ 233

[Close]

BLAST of CSPI02G02630 vs. NCBI nr
Match: TYJ97900.1 (putative prolyl 4-hydroxylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 271.2 bits (692), Expect = 7.7e-69
Identity = 143/203 (70.44%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           M+RSLVS Q  +W+G+VSS RTS+GRFL KGQNQLVRRIEKRIAEFTFIPVENGEGLSIL
Sbjct: 256 MKRSLVSDQK-SWKGLVSSHRTSTGRFLVKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 315

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYEVGQKF+PH+DY+ P+SF+FK+LGQ+           +   ++ +S VKEG ATVFP 
Sbjct: 316 HYEVGQKFDPHYDYSRPESFNFKTLGQR-----------IATLVMYLSDVKEGGATVFPA 375

Query: 121 AKK------CASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASS 180
           AKK      CASS+RRWWKKLPEYG + GLSVKPKMGDALLFWS+KPD TLDPTSLH   
Sbjct: 376 AKKCPVSKQCASSSRRWWKKLPEYGGE-GLSVKPKMGDALLFWSLKPDSTLDPTSLH--- 435

Query: 181 PVVKGDKWVGVKLMHVKAKDLTQ 198
            V++GDKWVGVKLMHV  KDLTQ
Sbjct: 436 -VIEGDKWVGVKLMHV--KDLTQ 439

[Close]

BLAST of CSPI02G02630 vs. NCBI nr
Match: KAE8651548.1 (hypothetical protein Csa_019366 [Cucumis sativus])

HSP 1 Score: 270.0 bits (689), Expect = 1.7e-68
Identity = 132/156 (84.62%), Postives = 138/156 (88.46%), Query Frame = 0


Query: 16  VVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYT 75
           ++S RRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYT
Sbjct: 27  IISCRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYT 86

Query: 76  HPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPEAKKCASSARRWWKKL 135
           HPDSFSFKSLGQ+               ++ +SGVKEG ATVFPEAKKCASSARRWWKKL
Sbjct: 87  HPDSFSFKSLGQRN-----------ATLVMYLSGVKEGGATVFPEAKKCASSARRWWKKL 146

Query: 136 PEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLH 172
           PEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLH
Sbjct: 147 PEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLH 171

[Close]

BLAST of CSPI02G02630 vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 1.7e-45
Identity = 98/190 (51.58%), Postives = 128/190 (67.37%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           ME+S V  + T  +   S  RTSSG FLA+G+++ +R IEKRI++FTFIPVE+GEGL +L
Sbjct: 110 MEKSTVVDEKTG-KSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVL 169

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYE+GQK+EPH+DY   D ++ ++ GQ+           +   L+ +S V+EG  TVFP 
Sbjct: 170 HYEIGQKYEPHYDY-FMDEYNTRNGGQR-----------IATVLMYLSDVEEGGETVFPA 229

Query: 121 AKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGD 180
           AK    SA  WW +L E GK  GLSVKPKMGDALLFWS+ PD TLDP+SLH    V+KG+
Sbjct: 230 AKG-NYSAVPWWNELSECGK-GGLSVKPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGN 284

Query: 181 KWVGVKLMHV 191
           KW   K + V
Sbjct: 290 KWSSTKWLRV 284

[Close]

BLAST of CSPI02G02630 vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 179.5 bits (454), Expect = 2.8e-45
Identity = 91/170 (53.53%), Postives = 119/170 (70.00%), Query Frame = 0


Query: 21  RTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHDYTHPDSF 80
           RTSSG FL +G++++++ IEKRIA++TFIP ++GEGL +LHYE GQK+EPH+DY   D F
Sbjct: 127 RTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDY-FVDEF 186

Query: 81  SFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPEAKKCASSARRWWKKLPEYGK 140
           + K+ GQ+M              L+ +S V+EG  TVFP A    SS   W+ +L E GK
Sbjct: 187 NTKNGGQRM-----------ATMLMYLSDVEEGGETVFPAANMNFSSV-PWYNELSECGK 246

Query: 141 DNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGDKWVGVKLMHV 191
             GLSVKP+MGDALLFWS++PD TLDPTSLH   PV++G+KW   K MHV
Sbjct: 247 -KGLSVKPRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHV 282

[Close]

BLAST of CSPI02G02630 vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 3.5e-43
Identity = 90/177 (50.85%), Postives = 115/177 (64.97%), Query Frame = 0


Query: 14  EGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSILHYEVGQKFEPHHD 73
           + + S  RTSSG FL +G +++V  IE RI++FTFIP ENGEGL +LHYEVGQ++EPHHD
Sbjct: 124 KSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHHD 183

Query: 74  YTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPEAKKCASSARRWWK 133
           Y   D F+ +  GQ+           +   L+ +S V EG  TVFP AK   S    WW 
Sbjct: 184 YFF-DEFNVRKGGQR-----------IATVLMYLSDVDEGGETVFPAAKGNVSDV-PWWD 243

Query: 134 KLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKGDKWVGVKLMHV 191
           +L + GK+ GLSV PK  DALLFWS+KPD +LDP+SLH   PV+KG+KW   K  HV
Sbjct: 244 ELSQCGKE-GLSVLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHV 286

[Close]

BLAST of CSPI02G02630 vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 169.1 bits (427), Expect = 3.8e-42
Identity = 96/191 (50.26%), Postives = 125/191 (65.45%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSR-RTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSI 60
           M +S V  + T   G   SR RTSSG FL +G +++V  IEKRI++FTFIPVENGEGL +
Sbjct: 112 MVKSTVVDEKTG--GSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQV 171

Query: 61  LHYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFP 120
           LHY+VGQK+EPH+DY   D F+ K+ GQ+           +   L+ +S V +G  TVFP
Sbjct: 172 LHYQVGQKYEPHYDY-FLDEFNTKNGGQR-----------IATVLMYLSDVDDGGETVFP 231

Query: 121 EAKKCASSARRWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKG 180
            A+    SA  WW +L + GK+ GLSV PK  DALLFW+++PD +LDP+SLH   PVVKG
Sbjct: 232 AARG-NISAVPWWNELSKCGKE-GLSVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKG 286

Query: 181 DKWVGVKLMHV 191
           +KW   K  HV
Sbjct: 292 NKWSSTKWFHV 286

[Close]

BLAST of CSPI02G02630 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 140.6 bits (353), Expect = 1.5e-33
Identity = 80/193 (41.45%), Postives = 117/193 (60.62%), Query Frame = 0


Query: 1   MERSLVSAQNTNWEGVVSSRRTSSGRFLAKGQNQLVRRIEKRIAEFTFIPVENGEGLSIL 60
           +E+S+V A N + E V S  RTSSG FL+K Q+ +V  +E ++A +TF+P ENGE + IL
Sbjct: 88  LEKSMV-ADNDSGESVESEVRTSSGMFLSKRQDDIVSNVEAKLAAWTFLPEENGESMQIL 147

Query: 61  HYEVGQKFEPHHDYTHPDSFSFKSLGQKMPPSSCICKILLTPNLIEMSGVKEGVATVFPE 120
           HYE GQK+EPH DY H D  + +  G +           +   L+ +S V++G  TVFP 
Sbjct: 148 HYENGQKYEPHFDYFH-DQANLELGGHR-----------IATVLMYLSNVEKGGETVFPM 207

Query: 121 AKKCASSAR-RWWKKLPEYGKDNGLSVKPKMGDALLFWSVKPDGTLDPTSLHASSPVVKG 180
            K  A+  +   W +  +     G +VKP+ GDALLF+++ P+ T D  SLH S PVV+G
Sbjct: 208 WKGKATQLKDDSWTECAK----QGYAVKPRKGDALLFFNLHPNATTDSNSLHGSCPVVEG 263

Query: 181 DKWVGVKLMHVKA 193
           +KW   + +HVK+
Sbjct: 268 EKWSATRWIHVKS 263

The following BLAST results are available for this feature:

BLAST of CSPI02G02630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JZ24	2.3e-44	51.58	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
Q9LN20	4.0e-44	53.53	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
F4JNU8	4.9e-42	50.85	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]
Q24JN5	5.4e-41	50.26	Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1	[more]
Q8L970	2.1e-32	41.45	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]

BLAST of CSPI02G02630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIA3	2.6e-102	90.48	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G...	[more]
A0A5A7V5R0	9.5e-73	71.92	Methyltransferase-like protein 6 isoform X1 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3CEJ0	4.7e-72	71.43	probable prolyl 4-hydroxylase 3 OS=Cucumis melo OX=3656 GN=LOC103499590 PE=4 SV=...	[more]
A0A5D3BDG5	3.7e-69	70.44	Putative prolyl 4-hydroxylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A151S4M6	5.8e-46	60.00	Prolyl 4-hydroxylase subunit alpha-1 OS=Cajanus cajan OX=3821 GN=KK1_028532 PE=4...	[more]

BLAST of CSPI02G02630 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031736349.1	1.3e-95	89.50	probable prolyl 4-hydroxylase 10 [Cucumis sativus]	[more]
KAA0063572.1	2.0e-72	71.92	methyltransferase-like protein 6 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008460837.1	9.7e-72	71.43	PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis melo]	[more]
TYJ97900.1	7.7e-69	70.44	putative prolyl 4-hydroxylase 3 [Cucumis melo var. makuwa]	[more]
KAE8651548.1	1.7e-68	84.62	hypothetical protein Csa_019366 [Cucumis sativus]	[more]

BLAST of CSPI02G02630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66060.1	1.7e-45	51.58	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G20270.1	2.8e-45	53.53	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G35810.1	3.5e-43	50.85	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17720.1	3.8e-42	50.26	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G28480.1	1.5e-33	41.45	Oxoglutarate/iron-dependent oxygenase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 1..189 e-value: 2.6E-11 score: 53.6
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 58..185 e-value: 2.9E-7 score: 31.3
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 1..190 e-value: 1.5E-40 score: 141.2
None	No IPR available	PANTHER	PTHR10869:SF157	PROLYL 4-HYDROXYLASE 10-RELATED	coord: 1..193
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 1..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G02630.1	CSPI02G02630.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen