CSPI02G02620 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G02620
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMethyltransferase-like protein
LocationChr2: 1809869 .. 1813222 (+)
RNA-Seq ExpressionCSPI02G02620
SyntenyCSPI02G02620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCATCATGCAAATTTTAATCTTTCAGAATTTGATCGATTTTCTCTCTTCTTTGCACATTTGTTGGAGAATTGTTTGATCTTGAGTCTTCAATTTCGAAGAATTGAATGGGGGAAGCTGCCGAATATTTCTCCAAAGATTTCCAATGGGATGATCTTCGTATTCGGGTTGAGAACGACCCGAATTTGCAATACCATTTGCTTCCATTTGAGCCCTCCAATTCCGTTCCGTCAGTTTCTCCAGAAGCCGATTCAAACGCATGGAGCCGCTTTCACCTCCGTCACTCCTCCGGCAAATTCTTCAAGGTTTTGATTCCGTTTAGTATTTTGAACGTTTTTGTATTCGTAGCAACCTAACTTAACTTTGCTGTTTCTCTTAGTTTGGTTGTGATTTGAGTATTTTATATTTGGGGTTCAGGAAAGGCGTTATTTGTTGAAGGAATTCCCTGAACTAGTTTCTTGTAAGAAGTATTGTAAAGTTTTGGAGGTGGGTTGTGGTAATGGAAGCACAGTTATTCCAATCTTGCGGTAACCTCCAACGTATTCTACTTTTCCTCTGCATTACTTCTTAGTCACGTTCATCAATTTTGTTTTGTTTTTTTTAGAAATTTAGTTGATAAGTCTGTTTGTGTTTGCATTGTGCTTGCTTATATGAGATATTATTCAAAAATATTATCGTTGAAATTAATTAGGAAGTAGAGAAAAAGCAGTGTCAGTTGGAGGATTCTTTTAGAACAGGAGACTAAAAAGACAACTGTTATTGAATGGGCTCTTAAAGTTCCCACATTTTCTATTTTGCTCTTTAAATTTCGCCAATGTTTCTATGTGATATGCTATTGTTGTTTAGGTTAGCTTTCATTCAGGGGTGAAACGGAACATCTTTACAAGGCGAAGACAAACATAATCCATATCCCTTCTTGATTTAATTATGTCAAGTTCAGTAGCTTGTTTTAGGGTCCTTAATGTAACCTTGTAATGTGTTCCATTTTCAATGAGTTAGGAGTGAGGTGCCACATTATAAGAGAGATAATTGATCCTTCACTTCCGTTTGAGATTTTGATTTTGCATGATCCGCCCTACTCTATGCAGGTTCTCCTTTGTATTATCGGTAGGATATATGCTCAGATCTGTATATCACCATATACCCTTCATATTTCGTTAGAAGTCCAAAATTCTCATTTAGATTGATGTTAATCTATTTCATTAATAAAGTTATAGCTGATGGAAGACAATGATGATAGCAGTGCCTAGGTTGAGCCATTTACTGAGGGTCTAGTCCATGGAAATGATGTGTATTCTATACATTTTTCGAACTCTGTTCAAGAGTTGAATCATAGATATTTCTTCATGAAACTTTATTGGGTGTTATGAATGCATCTTGTGGGAGAAAGTATATGAAATAGAACACAGCCTTTGCAATGTTTTACGGACATCCCAATAATTGCAATGCATTTGTATGGTCAAAGGTAGAATAAATTTGAACAATTAAATATGCCATTGAGTTGTAGATCTTATTGCTAGCTATTCGTTATCCTTACACACGATTACTTTCTTTGTAGCGGGAACGAGAACATCATCATCTATGCTTGTGATTGTAGCACGGAAACTCTTGAGAGAGCTAAAGACATTTTAAATGATGCTGGCTTTGCATCTTTAAAAGACCGATTCCATCCATTCTATTGTGACTTTTCTATTAGTAAGTTTCCAACATGGTTGGCCTGCAATTCATGCCGTGGAAACACTTTCCAACAGCAGCGGAGTTTTACTACTCCAGGTTGTTATTTTATCTATCTCTTTAGACTCATTAACATGCAAGATTATTGCTTTTCTTTACAATCTTATTCATCTTTCTCTACGTTTTCAGAGAATGATGGATCTCAGGCCACTGGTTCATTTGCACTAGAAGAAAGTGGATGTTGCATTGGTGGGGTTGATTTTATTACTTTGGTTCGTACCTCTTTTAGTATATTAATATAATTTTTAATTTTATCAAAGGCTTCAAATTTTGATTCCTAATTGTGGAACATTTTTTATTTCCAAAAAAAGAAGTAACAAAAATAAAAGCTAAAAGGCTCTAAGGCATGAATGGTGATGGTGTGCTTTCGAAAATCCTACATGTCCTGCAACTATTGTTTTGATATTGAACTAGATTCCAACTCTTTTGACCAACATCACTAAGGAAAAAACCAATAATGTATGGTGTAGCCTAGCACTAACAAACTTTTTTCAGTTTTAATGTCTTCTTGAATCTTGGTAATGACAGTTATAATGTTCTTTGGCTATTTCAGATATTTACATTATCAGCAGTGCCTCTCCAGAGGATGCCAGCATCAATCAGAGAATGCTTTATGGTTTTGAAGCCTGGAGGCCTGCTCTTATTTCGGGATTATGGTAATCGATTATGATCAGACCAATGATGACGTATTGGTTTGCTTTAAATTCTAAAGTAAACTTCCTTTTTCCAAGTTCTAATTTCCAGGCACATGTAACATGTCTAATTTGATGTTCGGTTTAATGGAATGCAGGTCTATATGATATGACAATGCTTCGATTTGCACAAGATCAAAGAGTAGGATTCAGGGAGTATGTCAGATTAGATGGAACACGTTCTTATTTCTTCTGCTTAAATACCTTGAGAGATCTTTTTGCCAATGCAGGCTTTGTTGAGGTACTTATTCTATTTTGAGAAGTATCAAAGTTAGGGACGAAATAAGATTTAAACCAATGCATAAATGAAATGACAATTACGTTTAACATGTAGCTATCTATTATGGTTCATATCCCATCTGCTCATAACGATGATGATGGTGTCGGCCTAATTAAGTTTATATTAAGTTCATATCCCATCTGCTCATTGCAGCTCGAGCTGGAATATTGTTGTGTGAAGTCCTCAAATCGCCGAAATGGAAAGAGCATGGAGAGGGTGTGGGTTCATGGAAAATTCCAGAAGCCTGTCTAATGAATCAAACTAGAGGCACGGAGCAACATTTGGCGTTTCTTGGTGAGTGATTATGAATTTTCCTTAAAAGGATGAAGAGGATGGATTGGTAACTCTTCAGGGTGTGTCATGAGCATACTGTGGGGGCCAGAGCCCCTTTAAAACTTTGATTATCAAGAAAATGAACAAAAAACTTTGCTTCAACTTGAATTTGGATGAACTTTGAGGATTTTTTTTTATTGACTTATAATTAGAGGAGTTTAATGGTGTTCCCTGGGTTATGTTAGAAAATTGGAAGTGTTACAAATGGTATTGTTAGTTAAAGTTCTGTTGAGCAAAATTGCTCCAATTAATGCCCTATTAGATGAAGTTCATAAGTTTTCATGCAAAATCTTAGATCCACAAATGGAAGGAAGTTT

mRNA sequence

GCATCATGCAAATTTTAATCTTTCAGAATTTGATCGATTTTCTCTCTTCTTTGCACATTTGTTGGAGAATTGTTTGATCTTGAGTCTTCAATTTCGAAGAATTGAATGGGGGAAGCTGCCGAATATTTCTCCAAAGATTTCCAATGGGATGATCTTCGTATTCGGGTTGAGAACGACCCGAATTTGCAATACCATTTGCTTCCATTTGAGCCCTCCAATTCCGTTCCGTCAGTTTCTCCAGAAGCCGATTCAAACGCATGGAGCCGCTTTCACCTCCGTCACTCCTCCGGCAAATTCTTCAAGGAAAGGCGTTATTTGTTGAAGGAATTCCCTGAACTAGTTTCTTGTAAGAAGTATTGTAAAGTTTTGGAGGTGGGTTGTGGTAATGGAAGCACAGTTATTCCAATCTTGCGCGGGAACGAGAACATCATCATCTATGCTTGTGATTGTAGCACGGAAACTCTTGAGAGAGCTAAAGACATTTTAAATGATGCTGGCTTTGCATCTTTAAAAGACCGATTCCATCCATTCTATTGTGACTTTTCTATTAGTAAGTTTCCAACATGGTTGGCCTGCAATTCATGCCGTGGAAACACTTTCCAACAGCAGCGGAGTTTTACTACTCCAGAGAATGATGGATCTCAGGCCACTGGTTCATTTGCACTAGAAGAAAGTGGATGTTGCATTGGTGGGGTTGATTTTATTACTTTGATATTTACATTATCAGCAGTGCCTCTCCAGAGGATGCCAGCATCAATCAGAGAATGCTTTATGGTTTTGAAGCCTGGAGGCCTGCTCTTATTTCGGGATTATGGTCTATATGATATGACAATGCTTCGATTTGCACAAGATCAAAGAGTAGGATTCAGGGAGTATGTCAGATTAGATGGAACACGTTCTTATTTCTTCTGCTTAAATACCTTGAGAGATCTTTTTGCCAATGCAGGCTTTGTTGAGCTCGAGCTGGAATATTGTTGTGTGAAGTCCTCAAATCGCCGAAATGGAAAGAGCATGGAGAGGGTGTGGGTTCATGGAAAATTCCAGAAGCCTGTCTAATGAATCAAACTAGAGGCACGGAGCAACATTTGGCGTTTCTTGGTGAGTGATTATGAATTTTCCTTAAAAGGATGAAGAGGATGGATTGGTAACTCTTCAGGGTGTGTCATGAGCATACTGTGGGGGCCAGAGCCCCTTTAAAACTTTGATTATCAAGAAAATGAACAAAAAACTTTGCTTCAACTTGAATTTGGATGAACTTTGAGGATTTTTTTTTATTGACTTATAATTAGAGGAGTTTAATGGTGTTCCCTGGGTTATGTTAGAAAATTGGAAGTGTTACAAATGGTATTGTTAGTTAAAGTTCTGTTGAGCAAAATTGCTCCAATTAATGCCCTATTAGATGAAGTTCATAAGTTTTCATGCAAAATCTTAGATCCACAAATGGAAGGAAGTTT

Coding sequence (CDS)

ATGGGGGAAGCTGCCGAATATTTCTCCAAAGATTTCCAATGGGATGATCTTCGTATTCGGGTTGAGAACGACCCGAATTTGCAATACCATTTGCTTCCATTTGAGCCCTCCAATTCCGTTCCGTCAGTTTCTCCAGAAGCCGATTCAAACGCATGGAGCCGCTTTCACCTCCGTCACTCCTCCGGCAAATTCTTCAAGGAAAGGCGTTATTTGTTGAAGGAATTCCCTGAACTAGTTTCTTGTAAGAAGTATTGTAAAGTTTTGGAGGTGGGTTGTGGTAATGGAAGCACAGTTATTCCAATCTTGCGCGGGAACGAGAACATCATCATCTATGCTTGTGATTGTAGCACGGAAACTCTTGAGAGAGCTAAAGACATTTTAAATGATGCTGGCTTTGCATCTTTAAAAGACCGATTCCATCCATTCTATTGTGACTTTTCTATTAGTAAGTTTCCAACATGGTTGGCCTGCAATTCATGCCGTGGAAACACTTTCCAACAGCAGCGGAGTTTTACTACTCCAGAGAATGATGGATCTCAGGCCACTGGTTCATTTGCACTAGAAGAAAGTGGATGTTGCATTGGTGGGGTTGATTTTATTACTTTGATATTTACATTATCAGCAGTGCCTCTCCAGAGGATGCCAGCATCAATCAGAGAATGCTTTATGGTTTTGAAGCCTGGAGGCCTGCTCTTATTTCGGGATTATGGTCTATATGATATGACAATGCTTCGATTTGCACAAGATCAAAGAGTAGGATTCAGGGAGTATGTCAGATTAGATGGAACACGTTCTTATTTCTTCTGCTTAAATACCTTGAGAGATCTTTTTGCCAATGCAGGCTTTGTTGAGCTCGAGCTGGAATATTGTTGTGTGAAGTCCTCAAATCGCCGAAATGGAAAGAGCATGGAGAGGGTGTGGGTTCATGGAAAATTCCAGAAGCCTGTCTAA

Protein sequence

MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHSSGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETLERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYDMTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNGKSMERVWVHGKFQKPV*
Homology
BLAST of CSPI02G02620 vs. ExPASy Swiss-Prot
Match: Q6AXU8 (tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Rattus norvegicus OX=10116 GN=Mettl6 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-34
Identity = 98/274 (35.77%), Postives = 135/274 (49.27%), Query Frame = 0

Query: 46  EADSNAWSRFHLRHSSGKFFKERRYLLKEFPELVSCKKY----CKVLEVGCGNGSTVIPI 105
           EA  N W  F+ R+S+  FFK+R +  +EF EL SC++Y      +LE GCG G+ + P+
Sbjct: 40  EAQKN-WDLFYKRNST-NFFKDRHWTTREFEELRSCREYEGQKLTLLEAGCGVGNCLFPL 99

Query: 106 LRGNENIIIYACDCSTETLERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCR 165
           L  + NI  YACD S   ++  K               HP Y       F     C+  R
Sbjct: 100 LEEDSNIFAYACDFSPRAVDYVKQ--------------HPLYNAERCKVF----QCDLTR 159

Query: 166 GNTFQQQRSFTTPENDGSQATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIREC 225
            +          PE+                    VD +TLIF LSAV  ++M   +   
Sbjct: 160 DDLLDH----IPPES--------------------VDAVTLIFVLSAVHPEKMHLVLLNV 219

Query: 226 FMVLKPGGLLLFRDYGLYDMTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAG 285
           + VLKPG  +LFRDYGL D  MLRF    ++G   YVR DGTRSYFF    L  LF +AG
Sbjct: 220 YKVLKPGRSVLFRDYGLNDHAMLRFKAGSKLGENFYVRQDGTRSYFFTDEFLAKLFVDAG 269

Query: 286 FVELELEYCCVKSSNRRNGKSMERVWVHGKFQKP 316
           + E+  EY   ++ N++ G  + RV++  KF+KP
Sbjct: 280 YEEVVNEYVFRETVNKKEGLCVPRVFLQSKFRKP 269

BLAST of CSPI02G02620 vs. ExPASy Swiss-Prot
Match: Q8TCB7 (tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Homo sapiens OX=9606 GN=METTL6 PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 2.5e-34
Identity = 98/275 (35.64%), Postives = 135/275 (49.09%), Query Frame = 0

Query: 46  EADSNAWSRFHLRHSSGKFFKERRYLLKEFPELVSCKKY----CKVLEVGCGNGSTVIPI 105
           EA  N W  F+ R+S+  FFK+R +  +EF EL SC+++      +LE GCG G+ + P+
Sbjct: 40  EAQKN-WDLFYKRNST-NFFKDRHWTTREFEELRSCREFEDQKLTMLEAGCGVGNCLFPL 99

Query: 106 LRGNENIIIYACDCSTETLERAK-DILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSC 165
           L  + NI  YACD S   +E  K + L D       +R   F CD               
Sbjct: 100 LEEDPNIFAYACDFSPRAIEYVKQNPLYDT------ERCKVFQCDL-------------- 159

Query: 166 RGNTFQQQRSFTTPENDGSQATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRE 225
              T         PE+                    VD + LIF LSAV   +M   ++ 
Sbjct: 160 ---TKDDLLDHVPPES--------------------VDVVMLIFVLSAVHPDKMHLVLQN 219

Query: 226 CFMVLKPGGLLLFRDYGLYDMTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANA 285
            + VLKPG  +LFRDYGLYD  MLRF    ++G   YVR DGTRSYFF  + L  LF + 
Sbjct: 220 IYKVLKPGKSVLFRDYGLYDHAMLRFKASSKLGENFYVRQDGTRSYFFTDDFLAQLFMDT 269

Query: 286 GFVELELEYCCVKSSNRRNGKSMERVWVHGKFQKP 316
           G+ E+  EY   ++ N++ G  + RV++  KF KP
Sbjct: 280 GYEEVVNEYVFRETVNKKEGLCVPRVFLQSKFLKP 269

BLAST of CSPI02G02620 vs. ExPASy Swiss-Prot
Match: Q8BVH9 (tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Mus musculus OX=10090 GN=Mettl6 PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 2.5e-34
Identity = 97/274 (35.40%), Postives = 135/274 (49.27%), Query Frame = 0

Query: 46  EADSNAWSRFHLRHSSGKFFKERRYLLKEFPELVSCKKY----CKVLEVGCGNGSTVIPI 105
           EA  N W  F+ R+S+  FFK+R +  +EF EL SC++Y      +LE GCG G+ + P+
Sbjct: 40  EAQKN-WDLFYKRNST-NFFKDRHWTTREFEELRSCREYEGQKLTLLEAGCGVGNCLFPL 99

Query: 106 LRGNENIIIYACDCSTETLERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCR 165
           L  + N+  YACD S   ++  K               HP Y       F     C+  R
Sbjct: 100 LEEDLNLFAYACDFSPRAVDYVKQ--------------HPLYNAERCKVF----QCDLTR 159

Query: 166 GNTFQQQRSFTTPENDGSQATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIREC 225
            +          PE+                    VD +TLIF LSAV  ++M   +   
Sbjct: 160 DDLLDH----VPPES--------------------VDAVTLIFVLSAVHPEKMRLVLLNV 219

Query: 226 FMVLKPGGLLLFRDYGLYDMTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAG 285
           + VLKPG  +LFRDYGL D  MLRF    ++G   YVR DGTRSYFF    L  LF +AG
Sbjct: 220 YKVLKPGRSVLFRDYGLNDHAMLRFKAGSKLGENFYVRQDGTRSYFFTDEFLAQLFVDAG 269

Query: 286 FVELELEYCCVKSSNRRNGKSMERVWVHGKFQKP 316
           + E+  EY   ++ N++ G  + RV++  KF+KP
Sbjct: 280 YEEVVNEYVFRETVNKKEGLCVPRVFLQSKFRKP 269

BLAST of CSPI02G02620 vs. ExPASy Swiss-Prot
Match: Q5RDV8 (tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Pongo abelii OX=9601 GN=METTL6 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.4e-32
Identity = 97/275 (35.27%), Postives = 133/275 (48.36%), Query Frame = 0

Query: 46  EADSNAWSRFHLRHSSGKFFKERRYLLKEFPELVSCKKY----CKVLEVGCGNGSTVIPI 105
           EA  N W  F+ R+S+  FFK+R +  +EF EL SC+++      +LE G G G+ + P+
Sbjct: 40  EAQKN-WDLFYKRNST-NFFKDRHWTTREFEELRSCREFEDQKLTMLEAGRGVGNCLFPL 99

Query: 106 LRGNENIIIYACDCSTETLERAK-DILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSC 165
           L  + NI  YACD S   +E  K + L D       +R   F CD               
Sbjct: 100 LEEDPNIFAYACDFSPRAVEYVKQNPLYDT------ERCKVFQCDL-------------- 159

Query: 166 RGNTFQQQRSFTTPENDGSQATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRE 225
              T         PE+                    VD + LIF LSAV   +M   ++ 
Sbjct: 160 ---TKDDLLDHVPPES--------------------VDVVMLIFVLSAVHPDKMHLVLQN 219

Query: 226 CFMVLKPGGLLLFRDYGLYDMTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANA 285
            + VLKPG  +LFRDYGLYD  MLRF    ++G   YVR DGTRSYFF    L  LF + 
Sbjct: 220 IYKVLKPGKSVLFRDYGLYDHAMLRFKAGSKLGENFYVRQDGTRSYFFTDEFLAQLFMDT 269

Query: 286 GFVELELEYCCVKSSNRRNGKSMERVWVHGKFQKP 316
           G+ E+  EY   ++ N++ G  + RV++  KF KP
Sbjct: 280 GYEEVVNEYVFRETVNKKEGLCVPRVFLQSKFLKP 269

BLAST of CSPI02G02620 vs. ExPASy Swiss-Prot
Match: A8KBL7 (tRNA N(3)-methylcytidine methyltransferase METTL2 OS=Danio rerio OX=7955 GN=mettl2a PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.3e-29
Identity = 96/320 (30.00%), Postives = 140/320 (43.75%), Query Frame = 0

Query: 35  EPSNSVPSVSPEADSNA----WSRFHLRHSSGKFFKERRYLLKEFPELVSCKKYC----- 94
           E S  +P+   E   N     W+ F+  H + +FFK+R +L  EFPEL   +K+      
Sbjct: 72  ENSQPLPAEKQEEFDNRANEYWNDFYTIHEN-RFFKDRHWLFTEFPELAPQQKHLRGAEE 131

Query: 95  -----------------------------KVLEVGCGNGSTVIPILRGNEN--IIIYACD 154
                                        ++LEVGCG G+TV PIL+ N +  + +Y CD
Sbjct: 132 KESLEHMLNGEDISLNPTHDEFPGASASYRILEVGCGVGNTVFPILKTNNDPGLFVYCCD 191

Query: 155 CSTETLERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTP 214
            S+  ++  K              + P  C   +                          
Sbjct: 192 FSSTAVDLVKS----------NPEYDPSRCHAFV-------------------------- 251

Query: 215 ENDGSQATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFR 274
            +D S  +G + + +       +D I LIF LSA+  ++M  SI     +LKPGG+LL R
Sbjct: 252 -HDMSDESGEYPMPDH-----SLDVIVLIFVLSALHPEKMQKSINRLGRLLKPGGVLLLR 311

Query: 275 DYGLYDMTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKS 315
           DYG YDM  LRF + + +    YVR DGTR YFF  + L DLF++AG  +L+        
Sbjct: 312 DYGRYDMAQLRFKKGRCLSENFYVRGDGTRVYFFTQDELHDLFSSAGLEKLQNLVDRRLQ 348

BLAST of CSPI02G02620 vs. ExPASy TrEMBL
Match: A0A0A0LG62 (Methyltransferase-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G009600 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 3.9e-187
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRFAQDQRVGFREYVRLDGTRSYFFCL+TLRDLFANAGFVELELEYCCVKSSNRRNG
Sbjct: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLSTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           KSMERVWVHGKFQKPV
Sbjct: 301 KSMERVWVHGKFQKPV 316

BLAST of CSPI02G02620 vs. ExPASy TrEMBL
Match: A0A5D3BFS2 (Methyltransferase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001150 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.1e-185
Identity = 311/316 (98.42%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNS+PSVSPEADSNAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSIPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQ+SFTTPENDGS 
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQQSFTTPENDGSP 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGS ALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSLALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG
Sbjct: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           K+MERVWVHGKFQKPV
Sbjct: 301 KTMERVWVHGKFQKPV 316

BLAST of CSPI02G02620 vs. ExPASy TrEMBL
Match: A0A1S3C384 (Methyltransferase-like protein OS=Cucumis melo OX=3656 GN=LOC103496346 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 6.2e-185
Identity = 310/316 (98.10%), Postives = 313/316 (99.05%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNS+PSVSPEADSNAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSIPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQ+SFTTPENDGS 
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQQSFTTPENDGSP 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGS ALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSLALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRF QDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG
Sbjct: 241 MTMLRFVQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           K+MERVWVHGKFQKPV
Sbjct: 301 KTMERVWVHGKFQKPV 316

BLAST of CSPI02G02620 vs. ExPASy TrEMBL
Match: A0A1S4DYQ1 (Methyltransferase-like protein OS=Cucumis melo OX=3656 GN=LOC103496346 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 1.9e-173
Identity = 297/316 (93.99%), Postives = 300/316 (94.94%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNS+PSVSPEADSNAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSIPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILR             TETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILR-------------TETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQ+SFTTPENDGS 
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQQSFTTPENDGSP 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGS ALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSLALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRF QDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG
Sbjct: 241 MTMLRFVQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           K+MERVWVHGKFQKPV
Sbjct: 301 KTMERVWVHGKFQKPV 303

BLAST of CSPI02G02620 vs. ExPASy TrEMBL
Match: A0A6J1G4K4 (Methyltransferase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450669 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 7.9e-164
Identity = 278/316 (87.97%), Postives = 292/316 (92.41%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDF W++LRIRVENDP LQYHLLPF+P +S+PSVS EADS+AWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFDWEELRIRVENDPLLQYHLLPFQPCDSIPSVSAEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKK CKVLEVGCGNGST +PILRGNENI+IYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKNCKVLEVGCGNGSTALPILRGNENIVIYACDCSTETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAK+I+ND G ASLKDRF PFYCDFS SKFPTWLACNSCRGNT  QQ SFTTPE +GSQ
Sbjct: 121 ERAKEIINDVGIASLKDRFCPFYCDFSTSKFPTWLACNSCRGNTSLQQPSFTTPEKEGSQ 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           AT S   EES CCIGGVDF+TLIFTLSAVPLQRMPASIREC+M LKPGGLLLFRDYGLYD
Sbjct: 181 ATES---EESECCIGGVDFLTLIFTLSAVPLQRMPASIRECYMALKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRF QDQRVGF+EYVRLDGTRSYFFCLNTLRDLF  AGFVELELEYCCVKS NRRNG
Sbjct: 241 MTMLRFGQDQRVGFKEYVRLDGTRSYFFCLNTLRDLFVTAGFVELELEYCCVKSLNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           KSMERVWVHGKFQKPV
Sbjct: 301 KSMERVWVHGKFQKPV 313

BLAST of CSPI02G02620 vs. NCBI nr
Match: XP_011648734.1 (tRNA N(3)-methylcytidine methyltransferase METTL6 [Cucumis sativus] >KGN60773.1 hypothetical protein Csa_019433 [Cucumis sativus])

HSP 1 Score: 663.7 bits (1711), Expect = 8.0e-187
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRFAQDQRVGFREYVRLDGTRSYFFCL+TLRDLFANAGFVELELEYCCVKSSNRRNG
Sbjct: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLSTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           KSMERVWVHGKFQKPV
Sbjct: 301 KSMERVWVHGKFQKPV 316

BLAST of CSPI02G02620 vs. NCBI nr
Match: TYJ97899.1 (methyltransferase-like protein 6 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 657.9 bits (1696), Expect = 4.4e-185
Identity = 311/316 (98.42%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNS+PSVSPEADSNAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSIPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQ+SFTTPENDGS 
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQQSFTTPENDGSP 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGS ALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSLALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG
Sbjct: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           K+MERVWVHGKFQKPV
Sbjct: 301 KTMERVWVHGKFQKPV 316

BLAST of CSPI02G02620 vs. NCBI nr
Match: XP_008456399.1 (PREDICTED: methyltransferase-like protein 6 isoform X1 [Cucumis melo])

HSP 1 Score: 656.4 bits (1692), Expect = 1.3e-184
Identity = 310/316 (98.10%), Postives = 313/316 (99.05%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNS+PSVSPEADSNAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSIPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQ+SFTTPENDGS 
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQQSFTTPENDGSP 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGS ALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSLALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRF QDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG
Sbjct: 241 MTMLRFVQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           K+MERVWVHGKFQKPV
Sbjct: 301 KTMERVWVHGKFQKPV 316

BLAST of CSPI02G02620 vs. NCBI nr
Match: XP_038890924.1 (tRNA N(3)-methylcytidine methyltransferase METTL6 [Benincasa hispida])

HSP 1 Score: 625.5 bits (1612), Expect = 2.4e-175
Identity = 296/316 (93.67%), Postives = 304/316 (96.20%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDF+W+DLRIRVENDP LQYHLLPFEPSNSVPSVSPEADS+AWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFEWNDLRIRVENDPLLQYHLLPFEPSNSVPSVSPEADSDAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENI+IYACDCSTETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIVIYACDCSTETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAK+ILN+AG ASLKDRF PFYCDFSISKFPTWLACNSCR NTFQ Q SFTTPENDGS 
Sbjct: 121 ERAKEILNEAGIASLKDRFCPFYCDFSISKFPTWLACNSCRANTFQMQPSFTTPENDGSH 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGS ALEESGCCIGGVDF+TLIFTLSAVPLQRMPASIRECF VLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSCALEESGCCIGGVDFLTLIFTLSAVPLQRMPASIRECFTVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRF QDQRVGFREYVRLDGTRSYFFCLNTLRDLF NAGFVELELEYCCV+S NRRNG
Sbjct: 241 MTMLRFGQDQRVGFREYVRLDGTRSYFFCLNTLRDLFVNAGFVELELEYCCVRSLNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           KSMERVWVHGKFQKPV
Sbjct: 301 KSMERVWVHGKFQKPV 316

BLAST of CSPI02G02620 vs. NCBI nr
Match: XP_016901111.1 (PREDICTED: methyltransferase-like protein 6 isoform X2 [Cucumis melo])

HSP 1 Score: 618.2 bits (1593), Expect = 3.9e-173
Identity = 297/316 (93.99%), Postives = 300/316 (94.94%), Query Frame = 0

Query: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHS 60
           MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNS+PSVSPEADSNAWSRFHLRHS
Sbjct: 1   MGEAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSIPSVSPEADSNAWSRFHLRHS 60

Query: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETL 120
           SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILR             TETL
Sbjct: 61  SGKFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILR-------------TETL 120

Query: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQ 180
           ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQ+SFTTPENDGS 
Sbjct: 121 ERAKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQQSFTTPENDGSP 180

Query: 181 ATGSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240
           ATGS ALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD
Sbjct: 181 ATGSLALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYD 240

Query: 241 MTMLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300
           MTMLRF QDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG
Sbjct: 241 MTMLRFVQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNG 300

Query: 301 KSMERVWVHGKFQKPV 317
           K+MERVWVHGKFQKPV
Sbjct: 301 KTMERVWVHGKFQKPV 303

BLAST of CSPI02G02620 vs. TAIR 10
Match: AT1G54650.2 (Methyltransferase family protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.1e-109
Identity = 196/313 (62.62%), Postives = 230/313 (73.48%), Query Frame = 0

Query: 3   EAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHSSG 62
           EA EY SKDF+W+ L+  VENDP+L +HL       ++    P  DS  W  FH RHSSG
Sbjct: 6   EAGEYHSKDFEWEFLKNIVENDPSLSHHL--HHHHQNLSKYLP--DSQPWQDFHSRHSSG 65

Query: 63  KFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETLER 122
           KFFKERRYLLKEFPELVSC +  K+LE+GCGNGSTV+PILRG++NI +YACDCS++ L R
Sbjct: 66  KFFKERRYLLKEFPELVSCGENSKLLEIGCGNGSTVLPILRGSKNITVYACDCSSDALVR 125

Query: 123 AKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQAT 182
            K+ ++ A   S  D FH F CDFS S+FP W+AC+ CR + F    S            
Sbjct: 126 TKENIDRA--ISSVDNFHSFCCDFSTSEFPDWVACDRCR-DKFMLNHS------------ 185

Query: 183 GSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYDMT 242
             F   ES  CIGGVDF+TLIFTLSAVP +RMP +I+ECF VLKPGGLLLFRDYGLYDMT
Sbjct: 186 -GFGGSESKHCIGGVDFVTLIFTLSAVPKERMPRAIKECFAVLKPGGLLLFRDYGLYDMT 245

Query: 243 MLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNGKS 302
           MLRF  ++RVGFREYVR DGT SYFFCL+T R LF +AGF+E+ELEYCCVK+ NRR GK 
Sbjct: 246 MLRFEPEKRVGFREYVRSDGTLSYFFCLDTARKLFTDAGFIEVELEYCCVKAVNRRKGKD 298

Query: 303 MERVWVHGKFQKP 316
           M RVWVHGKFQKP
Sbjct: 306 MYRVWVHGKFQKP 298

BLAST of CSPI02G02620 vs. TAIR 10
Match: AT1G54650.1 (Methyltransferase family protein )

HSP 1 Score: 393.3 bits (1009), Expect = 1.9e-109
Identity = 196/313 (62.62%), Postives = 230/313 (73.48%), Query Frame = 0

Query: 3   EAAEYFSKDFQWDDLRIRVENDPNLQYHLLPFEPSNSVPSVSPEADSNAWSRFHLRHSSG 62
           EA EY SKDF+W+ L+  VENDP+L +HL       ++    P  DS  W  FH RHSSG
Sbjct: 6   EAGEYHSKDFEWEFLKNIVENDPSLSHHL--HHHHQNLSKYLP--DSQPWQDFHSRHSSG 65

Query: 63  KFFKERRYLLKEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETLER 122
           KFFKERRYLLKEFPELVSC +  K+LE+GCGNGSTV+PILRG++NI +YACDCS++ L R
Sbjct: 66  KFFKERRYLLKEFPELVSCGENSKLLEIGCGNGSTVLPILRGSKNITVYACDCSSDALVR 125

Query: 123 AKDILNDAGFASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQAT 182
            K+ ++ A   S  D FH F CDFS S+FP W+AC+ CR         F    + GS   
Sbjct: 126 TKENIDRA--ISSVDNFHSFCCDFSTSEFPDWVACDRCRD-------KFMLNHSGGS--- 185

Query: 183 GSFALEESGCCIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYDMT 242
                 ES  CIGGVDF+TLIFTLSAVP +RMP +I+ECF VLKPGGLLLFRDYGLYDMT
Sbjct: 186 ------ESKHCIGGVDFVTLIFTLSAVPKERMPRAIKECFAVLKPGGLLLFRDYGLYDMT 245

Query: 243 MLRFAQDQRVGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNGKS 302
           MLRF  ++RVGFREYVR DGT SYFFCL+T R LF +AGF+E+ELEYCCVK+ NRR GK 
Sbjct: 246 MLRFEPEKRVGFREYVRSDGTLSYFFCLDTARKLFTDAGFIEVELEYCCVKAVNRRKGKD 296

Query: 303 MERVWVHGKFQKP 316
           M RVWVHGKFQKP
Sbjct: 306 MYRVWVHGKFQKP 296

BLAST of CSPI02G02620 vs. TAIR 10
Match: AT2G26200.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.9e-32
Identity = 91/303 (30.03%), Postives = 137/303 (45.21%), Query Frame = 0

Query: 19  IRVENDPNLQYHLLPFEPSNSVPSVSP------EADSNAWSRFHLRHSSGKFFKERRYLL 78
           +++  +P      L   P+ +   VSP      E D+  +     +H   +FFK+R YL 
Sbjct: 5   LKIIEEPQKPIEKLQIYPTANA-GVSPFWRDKYERDAKKYWDIFYKHHGDRFFKDRHYLD 64

Query: 79  KEFPELVSCKKYCKVLEVGCGNGSTVIPILRGNENIIIYACDCSTETLERAKDILNDAGF 138
           KE+    S      +LEVGCG G+T+ P++    +I +YACD S   +E  K     A  
Sbjct: 65  KEWNSYFSVSGKSVILEVGCGAGNTIFPLIATYPDIFVYACDFSPRAVELVK-----AHD 124

Query: 139 ASLKDRFHPFYCDFSISKFPTWLACNSCRGNTFQQQRSFTTPENDGSQATGSFALEESGC 198
              + R   F CD +       ++ +S                                 
Sbjct: 125 EYTETRVCAFACDLTGDGLDKHISPSS--------------------------------- 184

Query: 199 CIGGVDFITLIFTLSAVPLQRMPASIRECFMVLKPGGLLLFRDYGLYDMTMLRFA-QDQR 258
               VD +T+IF LSAV  ++M + ++    VLKP G +LFRDY + D+   RF+ +DQR
Sbjct: 185 ----VDIVTMIFVLSAVSPEKMSSVLQNIRKVLKPNGCILFRDYAVGDLAQERFSGKDQR 244

Query: 259 VGFREYVRLDGTRSYFFCLNTLRDLFANAGFVELELEYCCVKSSNRRNGKSMERVWVHGK 315
           +    YVR DGTR+++F    L  LF+  GF   EL+ CC +  NR     M R WV   
Sbjct: 245 ISENFYVRGDGTRAFYFSNEFLETLFSEQGFEVEELDVCCKQVENRSRELVMNRRWVQAT 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6AXU81.1e-3435.77tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Rattus norvegicus OX=10116 ... [more]
Q8TCB72.5e-3435.64tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Homo sapiens OX=9606 GN=MET... [more]
Q8BVH92.5e-3435.40tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Mus musculus OX=10090 GN=Me... [more]
Q5RDV81.4e-3235.27tRNA N(3)-methylcytidine methyltransferase METTL6 OS=Pongo abelii OX=9601 GN=MET... [more]
A8KBL79.3e-2930.00tRNA N(3)-methylcytidine methyltransferase METTL2 OS=Danio rerio OX=7955 GN=mett... [more]
Match NameE-valueIdentityDescription
A0A0A0LG623.9e-18799.68Methyltransferase-like protein OS=Cucumis sativus OX=3659 GN=Csa_2G009600 PE=3 S... [more]
A0A5D3BFS22.1e-18598.42Methyltransferase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3C3846.2e-18598.10Methyltransferase-like protein OS=Cucumis melo OX=3656 GN=LOC103496346 PE=3 SV=1[more]
A0A1S4DYQ11.9e-17393.99Methyltransferase-like protein OS=Cucumis melo OX=3656 GN=LOC103496346 PE=3 SV=1[more]
A0A6J1G4K47.9e-16487.97Methyltransferase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450669 PE=... [more]
Match NameE-valueIdentityDescription
XP_011648734.18.0e-18799.68tRNA N(3)-methylcytidine methyltransferase METTL6 [Cucumis sativus] >KGN60773.1 ... [more]
TYJ97899.14.4e-18598.42methyltransferase-like protein 6 isoform X1 [Cucumis melo var. makuwa][more]
XP_008456399.11.3e-18498.10PREDICTED: methyltransferase-like protein 6 isoform X1 [Cucumis melo][more]
XP_038890924.12.4e-17593.67tRNA N(3)-methylcytidine methyltransferase METTL6 [Benincasa hispida][more]
XP_016901111.13.9e-17393.99PREDICTED: methyltransferase-like protein 6 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G54650.21.1e-10962.62Methyltransferase family protein [more]
AT1G54650.11.9e-10962.62Methyltransferase family protein [more]
AT2G26200.11.9e-3230.03S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 58..159
e-value: 3.7E-8
score: 35.2
NoneNo IPR availablePANTHERPTHR22809:SF5O-METHYLTRANSFERASE 3coord: 3..157
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 86..146
e-value: 0.00156897
score: 35.0983
IPR026113Methyltransferase-likePANTHERPTHR22809METHYLTRANSFERASE-RELATEDcoord: 3..157
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 48..145

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G02620.1CSPI02G02620.1mRNA
CSPI02G02620.2CSPI02G02620.2mRNA
CSPI02G02620.3CSPI02G02620.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001510 RNA methylation
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen