CSPI02G01650 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G01650
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionprotein NRT1/ PTR FAMILY 6.2
LocationChr2: 1150579 .. 1154377 (+)
RNA-Seq ExpressionCSPI02G01650
SyntenyCSPI02G01650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAAGCTGGCCTCCATCTTCATTATGACCAAATGTGACCTGTCTCAATCTAGCAATAGATTCATACTTTCAGTGATTATCTAATTTGCTAAGAAATGTCTATATAATAGGAAGTGCCATACAATGCCTATACAAACCGAATTGAAGAATACCTGAGGCAGCAGCGCCATAGGCTCACTTCTTACCTTACATTCTCTACCAAAAGAATGGTGAGTTCTTTTCAGTTCTTTGCAGTATTCGAAATAGGATCAATAACATTCATATATTTCTTCTTTTCAAGTAACCATCTCAATGGCTACAGGATGGAAAGCAGAGATTAACGGTTGCTGATGCTGTGGACTACAAAGGGTGTCCGGCAAACCGGTCTAAAACCGGTGGTTGGGTCCCGGCTGCTCTTATTGTAGGTACTATATTTTGTCTTATATCTTTGATACGCCCTTCTTCTTGAAGTTTTTCTGGAAATGAAACCAAAACCAGCTTCAAGAAAGTGAATAAAAGGTTGTCTTGACAAGAGTTTGACATGTTCCCATATCTCGCCTATAAATCTAGTTTGGACAAAAGGTGGTCTTAGATCATCAAGAACTACAAATTCTACTCTTGTCTGAAATACAAGATAGCTAATCCAGTTGTGAGTTTGGATGAGTTTCAAGATCAATGTCACTATTATTGACAGCTACATTTGCAGTCATAGCCTATGAGATGTTTTAGAAGGATGACCTACCAGTCAAGTTTAATAGGTAATTGGAGAAAAATGATGATCTTAATCATGAAAGATAGGATTTTAGGTTTTATCAAGTTTTGATTAGTAGACATCTTTAGCACATTCAGCATTCAGAGCCTTCTCAATCTCAGTTCAAATCAATATTTTCTATCTCTAGAAGTGAGAGGATGGGACCCTAAAACGAATGCCATAGACAACTTCAAATAATTCTGTCCATGACATTAATATTCAGACAATCAAGAATAGGAAAAGGACAAATATTATTAGCTGGAACTTTCCCACATTATTAAATGTAATTGTTATTGCCCATCTTCAATTATACTCCACTTTCTATAAATGAAAGAATATTCAATTCTGCTCACTCCTAGCTACACATAAAAAATGTCAATCTGGTGTAAAATTTGGTTCTTGTTTCTCCAAGAGAACTAAAAAAAATTGTAATCATAATGGAAGAAAAGATAACATCATTAAAAGAGAGTATGGAATTTTTGCTACAGAATGAATCAAACTCATAAGATCTTGGACAGGGATTGAACTATGCGAAAGACTTTCAACAATGGGAATAGCTGTAAATCTGGTGACTTACTTGGTGGGAACATTGCATCTACCTAGTGCAAATTCAGCCAACATTGTCACAGATTTCATGGGCACATGTTTCCTTCTTTCCATGCTTGGGGGTTTTCTGGCAGATTCTTTCCTAGGAAGATATAAGACAATAGCAATCTTTGCCTCAATACAGACACTGGTAAATATAGATTTTCTTCAGTCCATGTGACATTTGATTCGACAATCTTATTTACTCTTTTCATAACTCAACCATAATTGCTAAAATGAATTAGGGAACTGGAACTCTAGCAGTAATAACAAAGTTACCACAACTACATCCACCTCCTTGCCATCCTATTGCCAGCAAGAACTGCAAGCAAGCAAATGGGTTTCAAATGGGAATGATTTATTTGCCTCTGTATATAATTGCTCTAGGAACTGGGGGTATCAAATCCAGTGTTTCGGGATTTGGGACCGACCAATTTGATGAGAAAGATGATAAGGAGAAGGCTCAAATGGCGTATTTCTTCAACAGATTTTTTTTGTTCGTCAGCAGTGGGACTTTGTTAGCTGTGACAGTGCTTGTTTACTTGCAAGATGAAGTGGGCCGCAGTTGGGCTTATGGAATTTGTTCAGTTTCAATGTTCACAGCAATTTTAATATTTCTATGTGGTACTAAGAGATACCGATACAAGATAAGCATGGGAAGTCCGATAGTTCATATCTTCCAAGTTCTTGTTGCAGCAATAAACAAGAGGAAGATGGAACTTCAACTCAATGCTACTTTGCTATATGAAGATTCTGCTGCAACCTCAAGAATTGATCACACAAATCAATTCCAGTAAGTGATTTTTTGGCCTGACTAAACATACTTGAACTAACCACTACGTCCATTAAAATCAAAACATAAATCTGTGACAACATTTAATAAGTTATTGATTTAAAATGAAGGAACGCTTCATAAATCAGAATTTGGACAAGGGTATTAATTTGTTTTACTTTCTAGATTTCTAGACAAGGCTGCAATTGTTGCGGATGGAGATTTTGAGAAATCTGTAAGCTCAGCTCCCAATCCCTGGAAGCTCTGTTCAGTAACGAGGGTAGAGGAGGTAAAGATGATGATGAGACTGCTCCCAATATGGGCCACAACCATAATCTTCTGGACCACATATGCTCAGATGATCACTTTCTCAGTGGTTCAGGCATCCACAATGGAAAGATCATTAGGAAATTTCAAAATCCCTGCCGGCTCTCTACCTGTTTTCTTTGTGGCAGCCATTTTGATCACGCTAGCCTTTTATGATCGTCTTATTATGCCTCTTTGGAAGAAATGGAAAGGGCAACCAGGTAAATATGGAATCTTCATATTCTAGTATTATCCTCCATTCCCATTCTCGAAATTAACTCAGGGAAGAAAACAAAATCTTAAACTTCTATTTCTGATCATGTTCCAGTTTAAACAGGATTCCCCTCATTCAGGTTGGGTTACTATCAGAAATTGTCATCTCTAATGTTTTTTCAGAATATTTGATTTGTATGCACGCACGTATAATACTAGAAGTTTTGCAACTGACACCGTAAGTAGAAACATTTGAAAAACCAAAGTGAACTTCCATTGAATTATCAAAACTTTATTGATAGTTACAAAGTTTAAGAAGAACCCACGTATCGGAGAAGGATAATAAAACATCATTATATTTTTGGTTTAGGAAGCAGTTTATTCCCTTCTTACTAGAATTTTTGACAACGTGATTCGTCCATTTTTTTTCACAGGTTTTACAAACCTACAAAGGATTGCCATAGGTCTTATCCTGTCAACTTTCGGAATGGCAGCTGCAGCTTTGGTTGAGATGAAACGGCTCTCAGTGGCTAAAGCTGTGGGTAGATCCACTGCAACTTTGCCTTTAAGTGTGTTCCTACTTATTCCTCAGTTTTTCTTGGTGGGTTCTGGAGAAGCTTTCATCTATACAGGCCAGCTTGATTTCTTCATAACACAATCCCCAAAGGGAATGAAAACTATGAGCACCGGCCTCTTCCTCACAACTTTGTCACTTGGGTTCTTTGTTAGTAGTTTTCTGGTTGCAGTTGTGAAGAGCGTGACCGGGAGCATGGATGGACAGGGATGGCTTGCAGACAACATAAACTATGCTAGGCTCGATTGTTTCTATGGACTTCTTACCATATTAAGTACCATAAATTTTGTAGCATTTTTAGTTTGTGCAATATGGTACAAGCCACAGAAACCCAAACAACTTCTTGAGATGGAAACTAGTACAAATGGGGGATCTGGAGCTGAGAAATGCTAGTAAACTAAGCTAGATTTTGGATTTATTCATTTTGTTTCTTGTATTCCACATTACTCCAAAAGTGTGGATTGGTATGGGTGGAGCAGTAATAAGTACTCTTTTTATCAAAATAACTAAGTACTCTTGAGTTTTGATTGACTAGGGATCATTTTGTAAGGATAAGCAGCAGATCTCAGTTTCAATGGATATTTTCTTATTGAGTATACTAAACCTGCACTCTTGA

mRNA sequence

ATGACGAAGCTGGCCTCCATCTTCATTATGACCAAATGTGACCTGTCTCAATCTAGCAATAGATTCATACTTTCAGAAGTGCCATACAATGCCTATACAAACCGAATTGAAGAATACCTGAGGCAGCAGCGCCATAGGCTCACTTCTTACCTTACATTCTCTACCAAAAGAATGGATGGAAAGCAGAGATTAACGGTTGCTGATGCTGTGGACTACAAAGGGTGTCCGGCAAACCGGTCTAAAACCGGTGGTTGGGTCCCGGCTGCTCTTATTGTAGGGATTGAACTATGCGAAAGACTTTCAACAATGGGAATAGCTGTAAATCTGGTGACTTACTTGGTGGGAACATTGCATCTACCTAGTGCAAATTCAGCCAACATTGTCACAGATTTCATGGGCACATGTTTCCTTCTTTCCATGCTTGGGGGTTTTCTGGCAGATTCTTTCCTAGGAAGATATAAGACAATAGCAATCTTTGCCTCAATACAGACACTGGGAACTGGAACTCTAGCAGTAATAACAAAGTTACCACAACTACATCCACCTCCTTGCCATCCTATTGCCAGCAAGAACTGCAAGCAAGCAAATGGGTTTCAAATGGGAATGATTTATTTGCCTCTGTATATAATTGCTCTAGGAACTGGGGGTATCAAATCCAGTGTTTCGGGATTTGGGACCGACCAATTTGATGAGAAAGATGATAAGGAGAAGGCTCAAATGGCGTATTTCTTCAACAGATTTTTTTTGTTCGTCAGCAGTGGGACTTTGTTAGCTGTGACAGTGCTTGTTTACTTGCAAGATGAAGTGGGCCGCAGTTGGGCTTATGGAATTTGTTCAGTTTCAATGTTCACAGCAATTTTAATATTTCTATGTGGTACTAAGAGATACCGATACAAGATAAGCATGGGAAGTCCGATAGTTCATATCTTCCAAGTTCTTGTTGCAGCAATAAACAAGAGGAAGATGGAACTTCAACTCAATGCTACTTTGCTATATGAAGATTCTGCTGCAACCTCAAGAATTGATCACACAAATCAATTCCAATTTCTAGACAAGGCTGCAATTGTTGCGGATGGAGATTTTGAGAAATCTGTAAGCTCAGCTCCCAATCCCTGGAAGCTCTGTTCAGTAACGAGGGTAGAGGAGGTAAAGATGATGATGAGACTGCTCCCAATATGGGCCACAACCATAATCTTCTGGACCACATATGCTCAGATGATCACTTTCTCAGTGGTTCAGGCATCCACAATGGAAAGATCATTAGGAAATTTCAAAATCCCTGCCGGCTCTCTACCTGTTTTCTTTGTGGCAGCCATTTTGATCACGCTAGCCTTTTATGATCGTCTTATTATGCCTCTTTGGAAGAAATGGAAAGGGCAACCAGGTTTTACAAACCTACAAAGGATTGCCATAGGTCTTATCCTGTCAACTTTCGGAATGGCAGCTGCAGCTTTGGTTGAGATGAAACGGCTCTCAGTGGCTAAAGCTGTGGGTAGATCCACTGCAACTTTGCCTTTAAGTGTGTTCCTACTTATTCCTCAGTTTTTCTTGGTGGGTTCTGGAGAAGCTTTCATCTATACAGGCCAGCTTGATTTCTTCATAACACAATCCCCAAAGGGAATGAAAACTATGAGCACCGGCCTCTTCCTCACAACTTTGTCACTTGGGTTCTTTGTTAGTAGTTTTCTGGTTGCAGTTGTGAAGAGCGTGACCGGGAGCATGGATGGACAGGGATGGCTTGCAGACAACATAAACTATGCTAGGCTCGATTGTTTCTATGGACTTCTTACCATATTAAGTACCATAAATTTTGTAGCATTTTTAGTTTGTGCAATATGGTACAAGCCACAGAAACCCAAACAACTTCTTGAGATGGAAACTAGTACAAATGGGGGATCTGGAGCTGAGAAATGCTAGTAAACTAAGCTAGATTTTGGATTTATTCATTTTGTTTCTTGTATTCCACATTACTCCAAAAGTGTGGATTGGTATGGGTGGAGCAGTAATAAGTACTCTTTTTATCAAAATAACTAAGTACTCTTGAGTTTTGATTGACTAGGGATCATTTTGTAAGGATAAGCAGCAGATCTCAGTTTCAATGGATATTTTCTTATTGAGTATACTAAACCTGCACTCTTGA

Coding sequence (CDS)

ATGACGAAGCTGGCCTCCATCTTCATTATGACCAAATGTGACCTGTCTCAATCTAGCAATAGATTCATACTTTCAGAAGTGCCATACAATGCCTATACAAACCGAATTGAAGAATACCTGAGGCAGCAGCGCCATAGGCTCACTTCTTACCTTACATTCTCTACCAAAAGAATGGATGGAAAGCAGAGATTAACGGTTGCTGATGCTGTGGACTACAAAGGGTGTCCGGCAAACCGGTCTAAAACCGGTGGTTGGGTCCCGGCTGCTCTTATTGTAGGGATTGAACTATGCGAAAGACTTTCAACAATGGGAATAGCTGTAAATCTGGTGACTTACTTGGTGGGAACATTGCATCTACCTAGTGCAAATTCAGCCAACATTGTCACAGATTTCATGGGCACATGTTTCCTTCTTTCCATGCTTGGGGGTTTTCTGGCAGATTCTTTCCTAGGAAGATATAAGACAATAGCAATCTTTGCCTCAATACAGACACTGGGAACTGGAACTCTAGCAGTAATAACAAAGTTACCACAACTACATCCACCTCCTTGCCATCCTATTGCCAGCAAGAACTGCAAGCAAGCAAATGGGTTTCAAATGGGAATGATTTATTTGCCTCTGTATATAATTGCTCTAGGAACTGGGGGTATCAAATCCAGTGTTTCGGGATTTGGGACCGACCAATTTGATGAGAAAGATGATAAGGAGAAGGCTCAAATGGCGTATTTCTTCAACAGATTTTTTTTGTTCGTCAGCAGTGGGACTTTGTTAGCTGTGACAGTGCTTGTTTACTTGCAAGATGAAGTGGGCCGCAGTTGGGCTTATGGAATTTGTTCAGTTTCAATGTTCACAGCAATTTTAATATTTCTATGTGGTACTAAGAGATACCGATACAAGATAAGCATGGGAAGTCCGATAGTTCATATCTTCCAAGTTCTTGTTGCAGCAATAAACAAGAGGAAGATGGAACTTCAACTCAATGCTACTTTGCTATATGAAGATTCTGCTGCAACCTCAAGAATTGATCACACAAATCAATTCCAATTTCTAGACAAGGCTGCAATTGTTGCGGATGGAGATTTTGAGAAATCTGTAAGCTCAGCTCCCAATCCCTGGAAGCTCTGTTCAGTAACGAGGGTAGAGGAGGTAAAGATGATGATGAGACTGCTCCCAATATGGGCCACAACCATAATCTTCTGGACCACATATGCTCAGATGATCACTTTCTCAGTGGTTCAGGCATCCACAATGGAAAGATCATTAGGAAATTTCAAAATCCCTGCCGGCTCTCTACCTGTTTTCTTTGTGGCAGCCATTTTGATCACGCTAGCCTTTTATGATCGTCTTATTATGCCTCTTTGGAAGAAATGGAAAGGGCAACCAGGTTTTACAAACCTACAAAGGATTGCCATAGGTCTTATCCTGTCAACTTTCGGAATGGCAGCTGCAGCTTTGGTTGAGATGAAACGGCTCTCAGTGGCTAAAGCTGTGGGTAGATCCACTGCAACTTTGCCTTTAAGTGTGTTCCTACTTATTCCTCAGTTTTTCTTGGTGGGTTCTGGAGAAGCTTTCATCTATACAGGCCAGCTTGATTTCTTCATAACACAATCCCCAAAGGGAATGAAAACTATGAGCACCGGCCTCTTCCTCACAACTTTGTCACTTGGGTTCTTTGTTAGTAGTTTTCTGGTTGCAGTTGTGAAGAGCGTGACCGGGAGCATGGATGGACAGGGATGGCTTGCAGACAACATAAACTATGCTAGGCTCGATTGTTTCTATGGACTTCTTACCATATTAAGTACCATAAATTTTGTAGCATTTTTAGTTTGTGCAATATGGTACAAGCCACAGAAACCCAAACAACTTCTTGAGATGGAAACTAGTACAAATGGGGGATCTGGAGCTGAGAAATGCTAG

Protein sequence

MTKLASIFIMTKCDLSQSSNRFILSEVPYNAYTNRIEEYLRQQRHRLTSYLTFSTKRMDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTLHLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVADGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTMERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTFGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLTILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC*
Homology
BLAST of CSPI02G01650 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 7.7e-240
Identity = 428/584 (73.29%), Postives = 490/584 (83.90%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           M+ K   TVADAVDYKG PA++SKTGGW+ AALI+GIE+ ERLSTMGIAVNLVTYL+ T+
Sbjct: 1   MESKGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETM 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPS+ SANIVTDFMGT FLL +LGGFLADSFLGR+KTI IF++IQ LGTG LAV TKLP
Sbjct: 61  HLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           +L PP CH    + C  A  FQM ++Y+ LY+IALGTGG+KSS+SGFG+DQFD+KD KEK
Sbjct: 121 ELRPPTCH--HGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           A MA+FFNRFF F+S GTLLAVTVLVY+QDEVGRSWAYGIC+VSM  AI+IFLCGTKRYR
Sbjct: 181 AHMAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YK S GSP+V IFQV+ AA  KRKMEL  +   LYED+    RI+HT+QF  LDKAAIVA
Sbjct: 241 YKKSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVA 300

Query: 358 DGDFEKSVS--SAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQAS 417
           +GDFE+++   + PNPWKL SVT+VEEVKMM+RLLPIWATTIIFWTTYAQMITFSV QAS
Sbjct: 301 EGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAS 360

Query: 418 TMERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLIL 477
           TM R++G+FKIPAGSL VFFVAAILITLA YDR IMP WKKWKG+PGF++LQRIAIGL+L
Sbjct: 361 TMRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVL 420

Query: 478 STFGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFIT 537
           ST GMAAAALVE KRLSVAK+   S  TLP+SVFLL+PQFFLVG+GEAFIYTGQLDFFIT
Sbjct: 421 STAGMAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFIT 480

Query: 538 QSPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGL 597
           QSPKGMKTMSTGLFLTTLSLGFFVSSFLV++VK VT +    GWLADNIN+ RLD FY L
Sbjct: 481 QSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWL 540

Query: 598 LTILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNG-GSGAEKC 639
           L ILS INFV +++CA+W+KP K K  +E E   NG G   E C
Sbjct: 541 LVILSGINFVVYIICALWFKPTKGKDSVEKE---NGKGFSVEDC 577

BLAST of CSPI02G01650 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 9.9e-163
Identity = 302/569 (53.08%), Postives = 402/569 (70.65%), Query Frame = 0

Query: 68  DAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTLHLPSANSANI 127
           DA D++G PA+RSKTGGW  AA+I+ IE  ERL+T+GI VNLVTYL GT+HL +A +AN 
Sbjct: 14  DAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANT 73

Query: 128 VTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCHPI 187
           VT+F+GT F+L +LGGF+AD+FLGRY TIAIFA+IQ  G   L + T +P L PP C+P 
Sbjct: 74  VTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPT 133

Query: 188 ASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRF 247
            S +C+QA+G Q+ ++YL LY+ ALGTGG+K+SVSGFG+DQFDE + KE+++M YFFNRF
Sbjct: 134 TSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRF 193

Query: 248 FLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKISMGSPIV 307
           F  ++ G+LLAVTVLVY+QD+VGR W YGIC+ ++  A+ +FL GT RYR+K  +GSP+ 
Sbjct: 194 FFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMT 253

Query: 308 HIFQVLVAAINKRKMELQLNATLLYE---------DSAATSRIDHTNQFQFLDKAAIVAD 367
            +  V+VAA   RK+EL  + + LY+               ++ HT QF+ LDKAAI   
Sbjct: 254 QVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI--- 313

Query: 368 GDFEKSV-SSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 427
            D E  V S+  N W L ++T VEEVK ++R+LPIWAT I+FWT +AQ+ T SV Q+ T+
Sbjct: 314 RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETL 373

Query: 428 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQP-GFTNLQRIAIGLILS 487
           +RS+G+F+IP  S+ VF+V  +L+T A YDR+ + L KK    P G   LQRI +GL   
Sbjct: 374 DRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFG 433

Query: 488 TFGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQ 547
           +  MA AALVE+KRL  A A G +  TLPL  +LLIPQ+ +VG GEA IYTGQLDFF+ +
Sbjct: 434 SMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRE 493

Query: 548 SPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLL 607
            PKGMK MSTGL L+TL+LGFF SS LV +V+  TG      W+AD++N  RL  FY L+
Sbjct: 494 CPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK--AHPWIADDLNKGRLYNFYWLV 553

Query: 608 TILSTINFVAFLVCAIWYKPQKPKQLLEM 626
            +L  +NF+ FLV + WY   K K+L E+
Sbjct: 554 AVLVALNFLIFLVFSKWY-VYKEKRLAEV 576

BLAST of CSPI02G01650 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.5e-142
Identity = 276/552 (50.00%), Postives = 372/552 (67.39%), Query Frame = 0

Query: 68  DAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTLHLPSANSANI 127
           +A DY+G P ++SKTGGW+ A LI+G EL ER+  MGI++NLVTYLVG LH+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 128 VTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCHPI 187
           VT+FMGT  LL +LGGFLAD+ LGRYK +AI AS+  LG   L V T +  + PP C   
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 188 --ASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFN 247
                 C +ANG Q+ ++Y+ LY IALG GGIKS+VSGFG+DQFD  D KE+ QM +FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 248 RFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKISMGSP 307
           RF+  +S G+L AV  LVY+QD VGR W YGI + +M  A ++ LCGTKRYR+K   GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 308 IVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVADGDFEKSV 367
              I++V   A  KRK     + +LL  +    + + HT   + LDKAAI  +   E S 
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL--NGYDNTTVPHTEMLKCLDKAAISKN---ESSP 315

Query: 368 SS----APNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTMERSL 427
           SS      +PW + +VT+VEEVK++M+L+PIWAT I+FWT Y+QM TF+V QA+ M+R L
Sbjct: 316 SSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKL 375

Query: 428 GNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQP-GFTNLQRIAIGLILSTFGM 487
           G+F +PAGS   F +  IL+  +  +R+ +PL ++   +P G T+LQRI +GL+ S   M
Sbjct: 376 GSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAM 435

Query: 488 AAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKG 547
           A AA++E  R   A    +      +S F L+PQ+FLVG+GEAF Y GQL+FFI ++P+ 
Sbjct: 436 AVAAVIENARREAAVNNDKK-----ISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPER 495

Query: 548 MKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLTILS 607
           MK+MSTGLFL+T+S+GFFVSS LV++V  VT     + WL  N+N ARL+ FY LL +L 
Sbjct: 496 MKSMSTGLFLSTISMGFFVSSLLVSLVDRVT----DKSWLRSNLNKARLNYFYWLLVVLG 553

Query: 608 TINFVAFLVCAI 613
            +NF+ F+V A+
Sbjct: 556 ALNFLIFIVFAM 553

BLAST of CSPI02G01650 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 2.4e-116
Identity = 235/570 (41.23%), Postives = 346/570 (60.70%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           M+ K   T    VD    PAN+ KTG W     I+G E CERL+  G+  NLV YL   L
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           +  +A +AN VT++ GTC++  ++G F+AD++LGRY TIA F  I   G   L +   +P
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
            L P  C+   +  C   N  Q  + ++ LY+IALGTGGIK  VS FG DQFDE D+ EK
Sbjct: 121 GLKPGNCN---ADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
            + + FFN F+  ++ G L+A TVLV++Q  VG  W +G+ +V+M  A+  F  G++ YR
Sbjct: 181 IKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRID------HTNQFQFLD 357
            +   GSP+  IFQV+VAA  K  +++  + +LL+E +   S I       HT+  +F D
Sbjct: 241 LQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFD 300

Query: 358 KAAIVADGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSV 417
           KAA+ +  D  K      NPW+LCSVT+VEE+K ++ LLP+WAT I+F T Y+QM T  V
Sbjct: 301 KAAVESQSDSIK--DGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFV 360

Query: 418 VQASTMERSLG-NFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKW-KGQPGFTNLQRI 477
           +Q +TM++ +G NF+IP+ SL +F   ++L     YD+ I+PL +K+ + + GFT LQR+
Sbjct: 361 LQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRM 420

Query: 478 AIGLILSTFGMAAAALVEMKRLSVAKAVGR-STATLPLSVFLLIPQFFLVGSGEAFIYTG 537
            IGL++S F M  A ++E+ RL   K         + +S+F  IPQ+ L+G  E F + G
Sbjct: 421 GIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIG 480

Query: 538 QLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYAR 597
           QL+FF  Q+P  M+++ + L LTT++LG ++S+ LV VV  +T      GW+ DN+N   
Sbjct: 481 QLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGH 540

Query: 598 LDCFYGLLTILSTINFVAFLVCAIWYKPQK 619
           LD F+ LL  LS +NF+ +L  +  YK +K
Sbjct: 541 LDYFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of CSPI02G01650 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 9.1e-116
Identity = 227/569 (39.89%), Postives = 353/569 (62.04%), Query Frame = 0

Query: 59  DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTLH 118
           D K   T    +D    PAN++KTG W     I+G E CERL+  G++ NL+ YL   ++
Sbjct: 3   DDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMN 62

Query: 119 LPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQ 178
           + + +++  V+++ GTC+   ++G F+AD++LGRY TIA F  I   G   L +   +P 
Sbjct: 63  MENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPG 122

Query: 179 LHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKA 238
           L P      + + C    G Q  + ++ LY+IALGTGGIK  VS FG DQFD+ D+KEK 
Sbjct: 123 LTP----TCSGETCHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKE 182

Query: 239 QMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRY 298
             + FFN F+  ++ G ++A +VLV++Q  VG  W  G+ +V+M  A++ F  G+  YR 
Sbjct: 183 SKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRL 242

Query: 299 KISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATS------RIDHTNQFQFLDK 358
           +   GSP+  + QV+VA+  K K+++  + +LLYE+  A S      +++HT    F DK
Sbjct: 243 QKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDK 302

Query: 359 AAIVADGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVV 418
           AA+  + D + +  S  + WKLC+VT+VEE+K ++RLLPIWAT I+F + Y+QM T  V+
Sbjct: 303 AAVETESDNKGAAKS--SSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVL 362

Query: 419 QASTMERSLG-NFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKG-QPGFTNLQRIA 478
           Q +T+++ +G NFKIP+ SL +F   ++L     YD+LI+P  +K+ G + GFT LQRI 
Sbjct: 363 QGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIG 422

Query: 479 IGLILSTFGMAAAALVEMKRLSVAKAVG-RSTATLPLSVFLLIPQFFLVGSGEAFIYTGQ 538
           IGL++S F M +A ++E+ RL+  +     +  T+P+++F  +PQ+FLVG  E F + GQ
Sbjct: 423 IGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQ 482

Query: 539 LDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARL 598
           L+FF  Q+P  M+++ + L LT ++ G ++S+FLV +V  VT S    GW+A N+N   L
Sbjct: 483 LEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHL 542

Query: 599 DCFYGLLTILSTINFVAFLVCAIWYKPQK 619
           D F+ LL  LS +NF+ +L  A WY  +K
Sbjct: 543 DYFFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of CSPI02G01650 vs. ExPASy TrEMBL
Match: A0A0A0LKY6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G006170 PE=3 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 580/581 (99.83%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAAL+VGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALVVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP
Sbjct: 61  HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. ExPASy TrEMBL
Match: J7LKJ6 (Nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.4A PE=2 SV=1)

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 579/581 (99.66%), Postives = 580/581 (99.83%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP
Sbjct: 61  HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVE+KRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEIKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILS INFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSAINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. ExPASy TrEMBL
Match: A0A5A7V7F5 (Protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001640 PE=3 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 566/581 (97.42%), Postives = 572/581 (98.45%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSA SANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFA+IQTLGTGTLAVITKLP
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPI SKNCKQANGFQMGMIYLPLY+IALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIGSKNCKQANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YK SMGSPIVHIFQV+VAAINKR MELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRSMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           +GDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 EGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSL VFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVEMKRLSVAKAVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVK VTGSMDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLA 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILS INFVAFLVCA+WYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSAINFVAFLVCAVWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. ExPASy TrEMBL
Match: A0A1S3C670 (protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo OX=3656 GN=LOC103497318 PE=3 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 566/581 (97.42%), Postives = 572/581 (98.45%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSA SANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFA+IQTLGTGTLAVITKLP
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPI SKNCKQANGFQMGMIYLPLY+IALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIGSKNCKQANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YK SMGSPIVHIFQV+VAAINKR MELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRSMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           +GDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 EGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSL VFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVEMKRLSVAKAVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVK VTGSMDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLA 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILS INFVAFLVCA+WYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSAINFVAFLVCAVWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. ExPASy TrEMBL
Match: A0A5D3BHY8 (Protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00180 PE=3 SV=1)

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 566/581 (97.42%), Postives = 572/581 (98.45%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSA SANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFA+IQTLGTGTLAVITKLP
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPI SKNCKQANGFQMGMIYLPLY+IALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIGSKNCKQANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YK SMGSPIVHIFQV+VAAINKR MELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRTMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           +GDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 EGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSL VFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVEMKRLSVAKAVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVK VTGSMDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLA 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILS INFVAFLVCA+WYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSAINFVAFLVCAVWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. NCBI nr
Match: XP_011648676.1 (protein NRT1/ PTR FAMILY 6.2 isoform X1 [Cucumis sativus])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 580/581 (99.83%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAAL+VGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALVVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP
Sbjct: 61  HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. NCBI nr
Match: AFR11354.1 (nitrate transporter [Cucumis sativus])

HSP 1 Score: 1139.4 bits (2946), Expect = 0.0e+00
Identity = 579/581 (99.66%), Postives = 580/581 (99.83%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP
Sbjct: 61  HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVE+KRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEIKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILS INFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSAINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. NCBI nr
Match: XP_008457678.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.2 [Cucumis melo] >KAA0061599.1 protein NRT1/ PTR FAMILY 6.2 [Cucumis melo var. makuwa])

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 566/581 (97.42%), Postives = 572/581 (98.45%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSA SANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFA+IQTLGTGTLAVITKLP
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPI SKNCKQANGFQMGMIYLPLY+IALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIGSKNCKQANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YK SMGSPIVHIFQV+VAAINKR MELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRSMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           +GDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 EGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSL VFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVEMKRLSVAKAVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVK VTGSMDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLA 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILS INFVAFLVCA+WYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSAINFVAFLVCAVWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. NCBI nr
Match: TYJ97808.1 (protein NRT1/ PTR FAMILY 6.2 [Cucumis melo var. makuwa])

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 566/581 (97.42%), Postives = 572/581 (98.45%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSA SANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFA+IQTLGTGTLAVITKLP
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHPI SKNCKQANGFQMGMIYLPLY+IALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPIGSKNCKQANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YK SMGSPIVHIFQV+VAAINKR MELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRTMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           +GDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 EGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLGNFKIPAGSL VFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGNFKIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMAAAALVEMKRLSVAKAVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMAAAALVEMKRLSVAKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVK VTGSMDGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLA 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILS INFVAFLVCA+WYKPQKPKQLLEMETSTNGGSGAEKC
Sbjct: 541 ILSAINFVAFLVCAVWYKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of CSPI02G01650 vs. NCBI nr
Match: XP_038890346.1 (protein NRT1/ PTR FAMILY 6.2 [Benincasa hispida])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 552/581 (95.01%), Postives = 564/581 (97.07%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           MDGK+ LTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL
Sbjct: 1   MDGKRSLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPSA SANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP
Sbjct: 61  HLPSATSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           QLHPPPCHP ASKNCKQANGFQ+GMIYLPLY+IALGTGGIKSSVSGFGTDQFDEKDDKEK
Sbjct: 121 QLHPPPCHPSASKNCKQANGFQLGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAIL FLCGTKRYR
Sbjct: 181 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILTFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YK SMGSPIVHIFQV+VAAINKRKME QLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA
Sbjct: 241 YKKSMGSPIVHIFQVIVAAINKRKME-QLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 300

Query: 358 DGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 417
           +GDFEKSV SAPNPWKLC+VTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM
Sbjct: 301 EGDFEKSVCSAPNPWKLCTVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 360

Query: 418 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST 477
           ERSLG+F+IPAGSL VFFVAAILITLAFYD LIMPLWKKWKGQPGFTNLQRIAIGLILST
Sbjct: 361 ERSLGDFQIPAGSLTVFFVAAILITLAFYDLLIMPLWKKWKGQPGFTNLQRIAIGLILST 420

Query: 478 FGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 537
           FGMA AAL EMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS
Sbjct: 421 FGMATAALAEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQS 480

Query: 538 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLT 597
           PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVK +TGS DGQGWLADNINYARLDCFYGLL 
Sbjct: 481 PKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKRMTGSKDGQGWLADNINYARLDCFYGLLA 540

Query: 598 ILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNGGSGAEKC 639
           ILS I+FVAFL+CAIWY PQKPKQ+LEMETSTNGGS AEKC
Sbjct: 541 ILSGIDFVAFLICAIWYMPQKPKQVLEMETSTNGGSAAEKC 580

BLAST of CSPI02G01650 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 831.2 bits (2146), Expect = 5.4e-241
Identity = 428/584 (73.29%), Postives = 490/584 (83.90%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           M+ K   TVADAVDYKG PA++SKTGGW+ AALI+GIE+ ERLSTMGIAVNLVTYL+ T+
Sbjct: 1   MESKGSWTVADAVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETM 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           HLPS+ SANIVTDFMGT FLL +LGGFLADSFLGR+KTI IF++IQ LGTG LAV TKLP
Sbjct: 61  HLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
           +L PP CH    + C  A  FQM ++Y+ LY+IALGTGG+KSS+SGFG+DQFD+KD KEK
Sbjct: 121 ELRPPTCH--HGEACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
           A MA+FFNRFF F+S GTLLAVTVLVY+QDEVGRSWAYGIC+VSM  AI+IFLCGTKRYR
Sbjct: 181 AHMAFFFNRFFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVA 357
           YK S GSP+V IFQV+ AA  KRKMEL  +   LYED+    RI+HT+QF  LDKAAIVA
Sbjct: 241 YKKSQGSPVVQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVA 300

Query: 358 DGDFEKSVS--SAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQAS 417
           +GDFE+++   + PNPWKL SVT+VEEVKMM+RLLPIWATTIIFWTTYAQMITFSV QAS
Sbjct: 301 EGDFEQTLDGVAIPNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQAS 360

Query: 418 TMERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLIL 477
           TM R++G+FKIPAGSL VFFVAAILITLA YDR IMP WKKWKG+PGF++LQRIAIGL+L
Sbjct: 361 TMRRNIGSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVL 420

Query: 478 STFGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFIT 537
           ST GMAAAALVE KRLSVAK+   S  TLP+SVFLL+PQFFLVG+GEAFIYTGQLDFFIT
Sbjct: 421 STAGMAAAALVEQKRLSVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFIT 480

Query: 538 QSPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGL 597
           QSPKGMKTMSTGLFLTTLSLGFFVSSFLV++VK VT +    GWLADNIN+ RLD FY L
Sbjct: 481 QSPKGMKTMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWL 540

Query: 598 LTILSTINFVAFLVCAIWYKPQKPKQLLEMETSTNG-GSGAEKC 639
           L ILS INFV +++CA+W+KP K K  +E E   NG G   E C
Sbjct: 541 LVILSGINFVVYIICALWFKPTKGKDSVEKE---NGKGFSVEDC 577

BLAST of CSPI02G01650 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 575.1 bits (1481), Expect = 7.0e-164
Identity = 302/569 (53.08%), Postives = 402/569 (70.65%), Query Frame = 0

Query: 68  DAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTLHLPSANSANI 127
           DA D++G PA+RSKTGGW  AA+I+ IE  ERL+T+GI VNLVTYL GT+HL +A +AN 
Sbjct: 14  DAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANT 73

Query: 128 VTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCHPI 187
           VT+F+GT F+L +LGGF+AD+FLGRY TIAIFA+IQ  G   L + T +P L PP C+P 
Sbjct: 74  VTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPT 133

Query: 188 ASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRF 247
            S +C+QA+G Q+ ++YL LY+ ALGTGG+K+SVSGFG+DQFDE + KE+++M YFFNRF
Sbjct: 134 TSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRF 193

Query: 248 FLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKISMGSPIV 307
           F  ++ G+LLAVTVLVY+QD+VGR W YGIC+ ++  A+ +FL GT RYR+K  +GSP+ 
Sbjct: 194 FFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMT 253

Query: 308 HIFQVLVAAINKRKMELQLNATLLYE---------DSAATSRIDHTNQFQFLDKAAIVAD 367
            +  V+VAA   RK+EL  + + LY+               ++ HT QF+ LDKAAI   
Sbjct: 254 QVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI--- 313

Query: 368 GDFEKSV-SSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTM 427
            D E  V S+  N W L ++T VEEVK ++R+LPIWAT I+FWT +AQ+ T SV Q+ T+
Sbjct: 314 RDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETL 373

Query: 428 ERSLGNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQP-GFTNLQRIAIGLILS 487
           +RS+G+F+IP  S+ VF+V  +L+T A YDR+ + L KK    P G   LQRI +GL   
Sbjct: 374 DRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFG 433

Query: 488 TFGMAAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQ 547
           +  MA AALVE+KRL  A A G +  TLPL  +LLIPQ+ +VG GEA IYTGQLDFF+ +
Sbjct: 434 SMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRE 493

Query: 548 SPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLL 607
            PKGMK MSTGL L+TL+LGFF SS LV +V+  TG      W+AD++N  RL  FY L+
Sbjct: 494 CPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK--AHPWIADDLNKGRLYNFYWLV 553

Query: 608 TILSTINFVAFLVCAIWYKPQKPKQLLEM 626
            +L  +NF+ FLV + WY   K K+L E+
Sbjct: 554 AVLVALNFLIFLVFSKWY-VYKEKRLAEV 576

BLAST of CSPI02G01650 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 507.3 bits (1305), Expect = 1.8e-143
Identity = 276/552 (50.00%), Postives = 372/552 (67.39%), Query Frame = 0

Query: 68  DAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTLHLPSANSANI 127
           +A DY+G P ++SKTGGW+ A LI+G EL ER+  MGI++NLVTYLVG LH+ SA SA I
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTYLVGDLHISSAKSATI 75

Query: 128 VTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCHPI 187
           VT+FMGT  LL +LGGFLAD+ LGRYK +AI AS+  LG   L V T +  + PP C   
Sbjct: 76  VTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDF 135

Query: 188 --ASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFN 247
                 C +ANG Q+ ++Y+ LY IALG GGIKS+VSGFG+DQFD  D KE+ QM +FFN
Sbjct: 136 RRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFN 195

Query: 248 RFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKISMGSP 307
           RF+  +S G+L AV  LVY+QD VGR W YGI + +M  A ++ LCGTKRYR+K   GSP
Sbjct: 196 RFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSP 255

Query: 308 IVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVADGDFEKSV 367
              I++V   A  KRK     + +LL  +    + + HT   + LDKAAI  +   E S 
Sbjct: 256 FTTIWRVGFLAWKKRKESYPAHPSLL--NGYDNTTVPHTEMLKCLDKAAISKN---ESSP 315

Query: 368 SS----APNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTMERSL 427
           SS      +PW + +VT+VEEVK++M+L+PIWAT I+FWT Y+QM TF+V QA+ M+R L
Sbjct: 316 SSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKL 375

Query: 428 GNFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKGQP-GFTNLQRIAIGLILSTFGM 487
           G+F +PAGS   F +  IL+  +  +R+ +PL ++   +P G T+LQRI +GL+ S   M
Sbjct: 376 GSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAM 435

Query: 488 AAAALVEMKRLSVAKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKG 547
           A AA++E  R   A    +      +S F L+PQ+FLVG+GEAF Y GQL+FFI ++P+ 
Sbjct: 436 AVAAVIENARREAAVNNDKK-----ISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPER 495

Query: 548 MKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLTILS 607
           MK+MSTGLFL+T+S+GFFVSS LV++V  VT     + WL  N+N ARL+ FY LL +L 
Sbjct: 496 MKSMSTGLFLSTISMGFFVSSLLVSLVDRVT----DKSWLRSNLNKARLNYFYWLLVVLG 553

Query: 608 TINFVAFLVCAI 613
            +NF+ F+V A+
Sbjct: 556 ALNFLIFIVFAM 553

BLAST of CSPI02G01650 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 421.0 bits (1081), Expect = 1.7e-117
Identity = 235/570 (41.23%), Postives = 346/570 (60.70%), Query Frame = 0

Query: 58  MDGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTL 117
           M+ K   T    VD    PAN+ KTG W     I+G E CERL+  G+  NLV YL   L
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRL 60

Query: 118 HLPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLP 177
           +  +A +AN VT++ GTC++  ++G F+AD++LGRY TIA F  I   G   L +   +P
Sbjct: 61  NQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVP 120

Query: 178 QLHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEK 237
            L P  C+   +  C   N  Q  + ++ LY+IALGTGGIK  VS FG DQFDE D+ EK
Sbjct: 121 GLKPGNCN---ADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEK 180

Query: 238 AQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYR 297
            + + FFN F+  ++ G L+A TVLV++Q  VG  W +G+ +V+M  A+  F  G++ YR
Sbjct: 181 IKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYR 240

Query: 298 YKISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATSRID------HTNQFQFLD 357
            +   GSP+  IFQV+VAA  K  +++  + +LL+E +   S I       HT+  +F D
Sbjct: 241 LQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFD 300

Query: 358 KAAIVADGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSV 417
           KAA+ +  D  K      NPW+LCSVT+VEE+K ++ LLP+WAT I+F T Y+QM T  V
Sbjct: 301 KAAVESQSDSIK--DGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFV 360

Query: 418 VQASTMERSLG-NFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKW-KGQPGFTNLQRI 477
           +Q +TM++ +G NF+IP+ SL +F   ++L     YD+ I+PL +K+ + + GFT LQR+
Sbjct: 361 LQGNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRM 420

Query: 478 AIGLILSTFGMAAAALVEMKRLSVAKAVGR-STATLPLSVFLLIPQFFLVGSGEAFIYTG 537
            IGL++S F M  A ++E+ RL   K         + +S+F  IPQ+ L+G  E F + G
Sbjct: 421 GIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIG 480

Query: 538 QLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYAR 597
           QL+FF  Q+P  M+++ + L LTT++LG ++S+ LV VV  +T      GW+ DN+N   
Sbjct: 481 QLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGH 540

Query: 598 LDCFYGLLTILSTINFVAFLVCAIWYKPQK 619
           LD F+ LL  LS +NF+ +L  +  YK +K
Sbjct: 541 LDYFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of CSPI02G01650 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 419.1 bits (1076), Expect = 6.5e-117
Identity = 227/569 (39.89%), Postives = 353/569 (62.04%), Query Frame = 0

Query: 59  DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIVGIELCERLSTMGIAVNLVTYLVGTLH 118
           D K   T    +D    PAN++KTG W     I+G E CERL+  G++ NL+ YL   ++
Sbjct: 3   DDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMN 62

Query: 119 LPSANSANIVTDFMGTCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQ 178
           + + +++  V+++ GTC+   ++G F+AD++LGRY TIA F  I   G   L +   +P 
Sbjct: 63  MENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPG 122

Query: 179 LHPPPCHPIASKNCKQANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKA 238
           L P      + + C    G Q  + ++ LY+IALGTGGIK  VS FG DQFD+ D+KEK 
Sbjct: 123 LTP----TCSGETCHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKE 182

Query: 239 QMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRY 298
             + FFN F+  ++ G ++A +VLV++Q  VG  W  G+ +V+M  A++ F  G+  YR 
Sbjct: 183 SKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRL 242

Query: 299 KISMGSPIVHIFQVLVAAINKRKMELQLNATLLYEDSAATS------RIDHTNQFQFLDK 358
           +   GSP+  + QV+VA+  K K+++  + +LLYE+  A S      +++HT    F DK
Sbjct: 243 QKPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDK 302

Query: 359 AAIVADGDFEKSVSSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVV 418
           AA+  + D + +  S  + WKLC+VT+VEE+K ++RLLPIWAT I+F + Y+QM T  V+
Sbjct: 303 AAVETESDNKGAAKS--SSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVL 362

Query: 419 QASTMERSLG-NFKIPAGSLPVFFVAAILITLAFYDRLIMPLWKKWKG-QPGFTNLQRIA 478
           Q +T+++ +G NFKIP+ SL +F   ++L     YD+LI+P  +K+ G + GFT LQRI 
Sbjct: 363 QGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIG 422

Query: 479 IGLILSTFGMAAAALVEMKRLSVAKAVG-RSTATLPLSVFLLIPQFFLVGSGEAFIYTGQ 538
           IGL++S F M +A ++E+ RL+  +     +  T+P+++F  +PQ+FLVG  E F + GQ
Sbjct: 423 IGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQ 482

Query: 539 LDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARL 598
           L+FF  Q+P  M+++ + L LT ++ G ++S+FLV +V  VT S    GW+A N+N   L
Sbjct: 483 LEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHL 542

Query: 599 DCFYGLLTILSTINFVAFLVCAIWYKPQK 619
           D F+ LL  LS +NF+ +L  A WY  +K
Sbjct: 543 DYFFWLLAGLSFLNFLVYLWIAKWYTYKK 564

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZY47.7e-24073.29Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q050859.9e-16353.08Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9LVE02.5e-14250.00Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q9M3902.4e-11641.23Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB89.1e-11639.89Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LKY60.0e+0099.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G006170 PE=3 SV=1[more]
J7LKJ60.0e+0099.66Nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.4A PE=2 SV=1[more]
A0A5A7V7F50.0e+0097.42Protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3C6700.0e+0097.42protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo OX=3656 GN=LOC103497318 PE=3 SV=1[more]
A0A5D3BHY80.0e+0097.42Protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
XP_011648676.10.0e+0099.83protein NRT1/ PTR FAMILY 6.2 isoform X1 [Cucumis sativus][more]
AFR11354.10.0e+0099.66nitrate transporter [Cucumis sativus][more]
XP_008457678.10.0e+0097.42PREDICTED: protein NRT1/ PTR FAMILY 6.2 [Cucumis melo] >KAA0061599.1 protein NRT... [more]
TYJ97808.10.0e+0097.42protein NRT1/ PTR FAMILY 6.2 [Cucumis melo var. makuwa][more]
XP_038890346.10.0e+0095.01protein NRT1/ PTR FAMILY 6.2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G26690.15.4e-24173.29Major facilitator superfamily protein [more]
AT1G12110.17.0e-16453.08nitrate transporter 1.1 [more]
AT3G21670.11.8e-14350.00Major facilitator superfamily protein [more]
AT3G54140.11.7e-11741.23peptide transporter 1 [more]
AT5G01180.16.5e-11739.89peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 153..577
e-value: 2.7E-102
score: 342.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 65..625
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 57..622
e-value: 1.0E-186
score: 623.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 76..621
NoneNo IPR availablePANTHERPTHR11654:SF85PROTEIN NRT1/ PTR FAMILY 6.2coord: 65..625
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 142..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G01650.1CSPI02G01650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity