CSPI02G00800 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI02G00800
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	6-phosphogluconate dehydrogenase family protein
Location	Chr2: 500351 .. 501825 (-)
RNA-Seq Expression	CSPI02G00800
Synteny	CSPI02G00800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGAACAAAAATAAAGATAAAAGTTAGATAGTACGAAACATTCTTAAGAATACATCAATCTTTTTTTTTTACAGTTCTTCGAATATGTCTTATTTTCAACCAAAATAGAAAGGAAAAATATTCGCAATCTCTCTTTTCTATAAAGGAAAAAGGATGCCGATGCAATTCTCTCTTCGATCTCTATACACTCAATCCCACAAACTAACCCCCACCCTATCCCTTCCTTTCCTCTCTTTCCTCCGTCGCACAATGTCAACTTCCGGCGACATCGTCAGCCCTTCAACCACCCGCGTCGGCTGGATCGGCACCGGCGTTATGGGCTGCTCCATGTGCTCTCACATCATCAAAGCCGGCTACAAACTCACCGTCTTCAATCGCACCATCTCAAAAGCCCAACCCCTCCTCGATTTGGGCGCCAATTTAGCCACTTCCCCTCTCGCCGTTGCCGCCCAGTCCGATGTCGTTTTCTCCATCGTCGGTTACCCATCCGACGTCCGCTCCGTCCTTCTCGACCCCACTTCCGGCGCACTTGCTGGCCTTCGCCCTGGCGGCGTCCTTATCGACATGACCACTTCCGAACCATCACTCGCCTCTGAAATCGCCGCCTCTGCAACCGCAAAGGGTTGTGGGGCAATCGACGCTCCGGTATCAGGCGGCGATCGCGGAGCGAAGAACGCCACTCTGGCGATCTTTGCCGGTGGCGATGAAGACGAAGTCCAGCGATTGAGTCCGTTGTTTTCTCTTTTAGGGAAAGTGAATTACATGGGTGAAAGTGGGAAAGGGCAATTTGCAAAGCTGGCGAATCAGATCACCATAGCTTCAACAATGGTGGGACTAGTGGAAGGGATGATTTATGCTAAGAAAGCGGGGCTTGATGTGGGTTTGTTCTTGAATGCTATATCAACAGGAGCAGCTGGATCAAAATCTCTGGATTTGTATGGAAGTAGGATTTTGAAAAGGGATTTTGAACCTGGGTTTTTTGTGAACCATTTTGTGAAGGATTTGGGAATTTGTTTGAAGGAATGTCAGAATATGGGGCTTGCTCTTCCTGGATTGGCATTGGCTCAACAACTTTATGTTTCGCTTAAGGCTTATGGGGAAGGGAATTTAGGGACTCAGGCACTGATTTTGTCTCTGGAACGGCTCAATAATGTTTCACTTGAGGGCAGTGGTTCTTCTTGATTTTAAGCTATTTGGTTATACCAATGGAGAAAGAGGTCATCATATAAATTTCACCTTTCCAAATTGTGGTTGTTTTATGACCATTTGTTGTTTATTACAATTATAAACAAGGAACTTATGAAGTTGTGTGAGTTTGGATTTAGTGATTTGCTAAACAATTGTGTTCTGGTAGATGAATTATGAGGAGAATCAAGACAAGATAGCACCAAAACTCATTTCAATTCTTTGCATCCATTAGTTCAATTTTCTCAAATCTTATTTTATTTTATAGATAAAAGATTTCTGTCTCTGC

mRNA sequence

AATGAACAAAAATAAAGATAAAAGTTAGATAGTACGAAACATTCTTAAGAATACATCAATCTTTTTTTTTTACAGTTCTTCGAATATGTCTTATTTTCAACCAAAATAGAAAGGAAAAATATTCGCAATCTCTCTTTTCTATAAAGGAAAAAGGATGCCGATGCAATTCTCTCTTCGATCTCTATACACTCAATCCCACAAACTAACCCCCACCCTATCCCTTCCTTTCCTCTCTTTCCTCCGTCGCACAATGTCAACTTCCGGCGACATCGTCAGCCCTTCAACCACCCGCGTCGGCTGGATCGGCACCGGCGTTATGGGCTGCTCCATGTGCTCTCACATCATCAAAGCCGGCTACAAACTCACCGTCTTCAATCGCACCATCTCAAAAGCCCAACCCCTCCTCGATTTGGGCGCCAATTTAGCCACTTCCCCTCTCGCCGTTGCCGCCCAGTCCGATGTCGTTTTCTCCATCGTCGGTTACCCATCCGACGTCCGCTCCGTCCTTCTCGACCCCACTTCCGGCGCACTTGCTGGCCTTCGCCCTGGCGGCGTCCTTATCGACATGACCACTTCCGAACCATCACTCGCCTCTGAAATCGCCGCCTCTGCAACCGCAAAGGGTTGTGGGGCAATCGACGCTCCGGTATCAGGCGGCGATCGCGGAGCGAAGAACGCCACTCTGGCGATCTTTGCCGGTGGCGATGAAGACGAAGTCCAGCGATTGAGTCCGTTGTTTTCTCTTTTAGGGAAAGTGAATTACATGGGTGAAAGTGGGAAAGGGCAATTTGCAAAGCTGGCGAATCAGATCACCATAGCTTCAACAATGGTGGGACTAGTGGAAGGGATGATTTATGCTAAGAAAGCGGGGCTTGATGTGGGTTTGTTCTTGAATGCTATATCAACAGGAGCAGCTGGATCAAAATCTCTGGATTTGTATGGAAGTAGGATTTTGAAAAGGGATTTTGAACCTGGGTTTTTTGTGAACCATTTTGTGAAGGATTTGGGAATTTGTTTGAAGGAATGTCAGAATATGGGGCTTGCTCTTCCTGGATTGGCATTGGCTCAACAACTTTATGTTTCGCTTAAGGCTTATGGGGAAGGGAATTTAGGGACTCAGGCACTGATTTTGTCTCTGGAACGGCTCAATAATGTTTCACTTGAGGGCAGTGGTTCTTCTTGATTTTAAGCTATTTGGTTATACCAATGGAGAAAGAGGTCATCATATAAATTTCACCTTTCCAAATTGTGGTTGTTTTATGACCATTTGTTGTTTATTACAATTATAAACAAGGAACTTATGAAGTTGTGTGAGTTTGGATTTAGTGATTTGCTAAACAATTGTGTTCTGGTAGATGAATTATGAGGAGAATCAAGACAAGATAGCACCAAAACTCATTTCAATTCTTTGCATCCATTAGTTCAATTTTCTCAAATCTTATTTTATTTTATAGATAAAAGATTTCTGTCTCTGC

Coding sequence (CDS)

ATGCCGATGCAATTCTCTCTTCGATCTCTATACACTCAATCCCACAAACTAACCCCCACCCTATCCCTTCCTTTCCTCTCTTTCCTCCGTCGCACAATGTCAACTTCCGGCGACATCGTCAGCCCTTCAACCACCCGCGTCGGCTGGATCGGCACCGGCGTTATGGGCTGCTCCATGTGCTCTCACATCATCAAAGCCGGCTACAAACTCACCGTCTTCAATCGCACCATCTCAAAAGCCCAACCCCTCCTCGATTTGGGCGCCAATTTAGCCACTTCCCCTCTCGCCGTTGCCGCCCAGTCCGATGTCGTTTTCTCCATCGTCGGTTACCCATCCGACGTCCGCTCCGTCCTTCTCGACCCCACTTCCGGCGCACTTGCTGGCCTTCGCCCTGGCGGCGTCCTTATCGACATGACCACTTCCGAACCATCACTCGCCTCTGAAATCGCCGCCTCTGCAACCGCAAAGGGTTGTGGGGCAATCGACGCTCCGGTATCAGGCGGCGATCGCGGAGCGAAGAACGCCACTCTGGCGATCTTTGCCGGTGGCGATGAAGACGAAGTCCAGCGATTGAGTCCGTTGTTTTCTCTTTTAGGGAAAGTGAATTACATGGGTGAAAGTGGGAAAGGGCAATTTGCAAAGCTGGCGAATCAGATCACCATAGCTTCAACAATGGTGGGACTAGTGGAAGGGATGATTTATGCTAAGAAAGCGGGGCTTGATGTGGGTTTGTTCTTGAATGCTATATCAACAGGAGCAGCTGGATCAAAATCTCTGGATTTGTATGGAAGTAGGATTTTGAAAAGGGATTTTGAACCTGGGTTTTTTGTGAACCATTTTGTGAAGGATTTGGGAATTTGTTTGAAGGAATGTCAGAATATGGGGCTTGCTCTTCCTGGATTGGCATTGGCTCAACAACTTTATGTTTCGCTTAAGGCTTATGGGGAAGGGAATTTAGGGACTCAGGCACTGATTTTGTCTCTGGAACGGCTCAATAATGTTTCACTTGAGGGCAGTGGTTCTTCTTGA

Protein sequence

MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGSS*

Homology

[Close]

BLAST of CSPI02G00800 vs. ExPASy Swiss-Prot
Match: Q9SZE1 (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.7e-135
Identity = 248/326 (76.07%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 19  PTLSLPFLSFLRRTMST---SGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNR 78
           P+ S+    FLRR+M++   S DI++PS T++GWIGTGVMG SMC H+IKAGY +TVFNR
Sbjct: 9   PSPSVVSSFFLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNR 68

Query: 79  TISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVL 138
           TISKAQ L+D+GAN+A SP +VA QSDVVF+IVGYPSDVR VLLDP SGAL+GLR GGVL
Sbjct: 69  TISKAQTLIDMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVL 128

Query: 139 IDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEDEVQRLSPLF 198
           +DMTTSEPSLA EIA +A+ K C +IDAPVSGGD GAKN  L+IFAGGDE  V+RL PLF
Sbjct: 129 VDMTTSEPSLAEEIAKAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLF 188

Query: 199 SLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAG 258
           SL+GKVN+MG SGKGQFAKLANQITIASTM+GLVEG+IYA KAGLDV  FL AISTGAAG
Sbjct: 189 SLMGKVNFMGTSGKGQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAG 248

Query: 259 SKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYG 318
           SKS+DLYG RILKRDF+PGF+VNHFVKDLGICL ECQ MGLALPGLALAQQLY+SLKA+G
Sbjct: 249 SKSIDLYGDRILKRDFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHG 308

Query: 319 EGNLGTQALILSLERLNNVSLEGSGS 342
           EG+LGTQAL+L+LERLNNVS++ S S
Sbjct: 309 EGDLGTQALLLALERLNNVSVQSSDS 334

[Close]

BLAST of CSPI02G00800 vs. ExPASy Swiss-Prot
Match: Q949M8 (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.2e-75
Identity = 149/297 (50.17%), Postives = 200/297 (67.34%), Query Frame = 0


Query: 39  IVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVA 98
           ++ PS TR+GWIG G+MG +M SHII AGY +TV+ R + K + L   GA +A SP  +A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL PGGV +DMT+S+P LA EI A A  + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 159 GAIDAPVSGGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQ 218
            A+DAPVSGGD GA+  TL IFAGGD + V+ LSP+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 219 ITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVN 278
           I  AS +VGL EG+++A+KAGLD   +L A+  GAAGS  + L+G  I+KRD+    F  
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +       A+PG AL++QL+  + A G+G LG Q ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

[Close]

BLAST of CSPI02G00800 vs. ExPASy Swiss-Prot
Match: Q9C991 (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.1e-70
Identity = 139/297 (46.80%), Postives = 195/297 (65.66%), Query Frame = 0


Query: 39  IVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVA 98
           ++ PS TR+GWIG G+MG +M SHI+ AGY +TV+ R + K + L   G   A SP  + 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL+PGGV +DMT+S+P LA EI A A  + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 159 GAIDAPVSGGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQ 218
            A+DAPVSGGD GA+   L IFAGGD + V+ L+P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 219 ITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVN 278
           I + S M+GL EG+++A+KAGLD   +L A+  GAAGS  + L+G  +  RD++   F  
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +      +A+PG AL +QL+  + A G+G LG Q ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

[Close]

BLAST of CSPI02G00800 vs. ExPASy Swiss-Prot
Match: O34948 (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 GN=ykwC PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.7e-66
Identity = 134/279 (48.03%), Postives = 190/279 (68.10%), Query Frame = 0


Query: 47  VGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFS 106
           +G+IG GVMG SM SHI+  G+ + V+ RT  KA+ +L  GA    +   ++ ++DV+ +
Sbjct: 5   IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADVIIT 64

Query: 107 IVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVS 166
           +VGYPSDV  V    ++G +   + G  LIDMTTS+PSLA +IA +A  K   A+DAPVS
Sbjct: 65  MVGYPSDVEEVYFG-SNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVS 124

Query: 167 GGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGK-VNYMGESGKGQFAKLANQITIASTM 226
           GGD GA+N TLAI  GG+++  +   P+FSL+G+ + Y G +G GQ  K+ NQI IA+ M
Sbjct: 125 GGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAAGM 184

Query: 227 VGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLG 286
           +G+ E M YA+K+GL+    L +I+TGAAGS SL     R+L+ +FEPGF+V HF+KD+G
Sbjct: 185 IGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKDMG 244

Query: 287 ICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQAL 325
           I L+E + MG  +PGL+LA+ LY  L A GE N GTQ++
Sbjct: 245 IALEEAELMGEEMPGLSLAKSLYDKLAAQGEENSGTQSI 282

[Close]

BLAST of CSPI02G00800 vs. ExPASy Swiss-Prot
Match: O33730 (Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=Sfri_1503 PE=3 SV=2)

HSP 1 Score: 174.5 bits (441), Expect = 2.1e-42
Identity = 107/287 (37.28%), Postives = 154/287 (53.66%), Query Frame = 0


Query: 46  RVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKA-QPLLDLGANLATSPLAVAAQSDVV 105
           +V +IG GVMG  M  H++K G+ +TV+NRT +KA Q +   G   A +P   A   D+V
Sbjct: 3   KVAFIGLGVMGFPMAGHLVKQGHDVTVYNRTGAKATQWVEQYGGKKADTPKDAAQGQDIV 62

Query: 106 FSIVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAP 165
           F+ VG   D+R V+L    G + G+  G +L+D TT+   +A EIAA         +DAP
Sbjct: 63  FTCVGNDDDLRQVVLG-EHGIVHGMHAGAILVDHTTASADVAREIAAYIEPLNIAFLDAP 122

Query: 166 VSGGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGK-VNYMGESGKGQFAKLANQITIAS 225
           VSGG  GA+N  L +  GGD+     + P+ S   +    +G  G GQ  K+ NQI IA 
Sbjct: 123 VSGGQAGAENGALTVMMGGDQAHFDTVKPVISAYSRCAELLGPVGAGQLTKMVNQICIAG 182

Query: 226 TMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKD 285
            + GL EG+ +AK AGLD    +  IS GAA S  ++     + +  ++ GF ++   KD
Sbjct: 183 VVQGLAEGLHFAKSAGLDGLKVIEVISKGAAQSWQMENRYKTMWQGQYDFGFAIDWMRKD 242

Query: 286 LGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSLER 331
           LGI L E +  G  LP  AL  Q Y  ++A       T +L+  LE+
Sbjct: 243 LGIALDEARRNGSHLPVAALVDQFYSEVQAMKGNRWDTSSLLARLEK 288

[Close]

BLAST of CSPI02G00800 vs. ExPASy TrEMBL
Match: A0A0A0LIQ8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000840 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 3.3e-184
Identity = 340/342 (99.42%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+QFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPIQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           P+SGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF
Sbjct: 121 PSSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL
Sbjct: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGSS 343
           LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGSS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGSS 342

[Close]

BLAST of CSPI02G00800 vs. ExPASy TrEMBL
Match: A0A1S3C8W8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498120 PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 7.2e-179
Identity = 329/341 (96.48%), Postives = 337/341 (98.83%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+QF+LRSLYTQS KLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQFTLRSLYTQSQKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGYKLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSEPSLASEIAA+ATAKGCGAIDAPVSGGDRGAKNATLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGGDE EV+RLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL
Sbjct: 181 AGGDEGEVRRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGS 342
           LALAQQLYVSLKAYGEGNLGTQALILS+ERLNNVSL+  GS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSVERLNNVSLDDGGS 341

[Close]

BLAST of CSPI02G00800 vs. ExPASy TrEMBL
Match: A0A6J1G2N8 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111450245 PE=4 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.2e-171
Identity = 317/349 (90.83%), Postives = 333/349 (95.42%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+Q SLRSLYTQSHKL PTLSLPFL +LRRTM+TSGDIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAG+KLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSEPSLASEIAA+  AKGC A+DAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGG+E EV+RL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLE-------GSGSS 343
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSL+       GSGSS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS 349

[Close]

BLAST of CSPI02G00800 vs. ExPASy TrEMBL
Match: A0A6J1KJW1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111493847 PE=4 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.2e-169
Identity = 314/346 (90.75%), Postives = 331/346 (95.66%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+Q SLRSLYTQSHKL PTLSL FL  LRRTM+TSGDIV+P+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLAPTLSLSFLPHLRRTMATSGDIVNPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAG+KLTVFNRTISKAQPL+DLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLVDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSEPSLASEIAA+A AKGC A+DAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGG+E EV+RL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLE-----GSGS 342
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSL+     GSGS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGS 346

[Close]

BLAST of CSPI02G00800 vs. ExPASy TrEMBL
Match: A0A5D3CJZ2 (Putative 3-hydroxyisobutyrate dehydrogenase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00590 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.1e-162
Identity = 300/309 (97.09%), Postives = 306/309 (99.03%), Query Frame = 0


Query: 33  MSTSGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLAT 92
           MSTSGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLA+
Sbjct: 1   MSTSGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLAS 60

Query: 93  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAAS 152
           SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVL+DMTTSEPSLASEIAA+
Sbjct: 61  SPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLVDMTTSEPSLASEIAAA 120

Query: 153 ATAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGKVNYMGESGKGQF 212
           ATAKGCGAIDAPVSGGDRGAKNATLAIFAGGDE EV+RLSPLFSLLGKVNYMGESGKGQF
Sbjct: 121 ATAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEGEVRRLSPLFSLLGKVNYMGESGKGQF 180

Query: 213 AKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 272
           AKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE
Sbjct: 181 AKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFE 240

Query: 273 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSLERLN 332
           PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILS+ERLN
Sbjct: 241 PGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSVERLN 300

Query: 333 NVSLEGSGS 342
           NVSL+  GS
Sbjct: 301 NVSLDDGGS 309

[Close]

BLAST of CSPI02G00800 vs. NCBI nr
Match: XP_004141022.2 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis sativus] >KGN60577.1 hypothetical protein Csa_019202 [Cucumis sativus])

HSP 1 Score: 654.1 bits (1686), Expect = 6.9e-184
Identity = 340/342 (99.42%), Postives = 342/342 (100.00%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+QFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPIQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           P+SGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF
Sbjct: 121 PSSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL
Sbjct: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGSS 343
           LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGSS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGSS 342

[Close]

BLAST of CSPI02G00800 vs. NCBI nr
Match: XP_008458836.1 (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis melo])

HSP 1 Score: 636.3 bits (1640), Expect = 1.5e-178
Identity = 329/341 (96.48%), Postives = 337/341 (98.83%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+QF+LRSLYTQS KLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQFTLRSLYTQSQKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAGYKLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSEPSLASEIAA+ATAKGCGAIDAPVSGGDRGAKNATLAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNATLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGGDE EV+RLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL
Sbjct: 181 AGGDEGEVRRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGSGS 342
           LALAQQLYVSLKAYGEGNLGTQALILS+ERLNNVSL+  GS
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSVERLNNVSLDDGGS 341

[Close]

BLAST of CSPI02G00800 vs. NCBI nr
Match: XP_038889572.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 615.5 bits (1586), Expect = 2.7e-172
Identity = 319/339 (94.10%), Postives = 331/339 (97.64%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+Q SLRSLYTQS++LT TLSLPFLSFLR TMSTS DIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSNQLTHTLSLPFLSFLRCTMSTSRDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAG+KLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSD+VFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDIVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSEPSLASEIAA+ATAKGCGAIDAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAATAKGCGAIDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGGDE EV+RL PLFSLLGKVNYMGE+GKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGDESEVRRLKPLFSLLGKVNYMGETGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLEGS 340
           LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLE +
Sbjct: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLESA 339

[Close]

BLAST of CSPI02G00800 vs. NCBI nr
Match: XP_023545872.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614.0 bits (1582), Expect = 7.9e-172
Identity = 320/349 (91.69%), Postives = 334/349 (95.70%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+Q SLRSLYTQSHKL PTLSLPFL +LRRTM+TSGDIVSPSTTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMTTSGDIVSPSTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAG+KLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSEPSLASEIAA+A AKGC A+DAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAAAAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGG+E EVQRL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGNEGEVQRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLE-------GSGSS 343
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSL+       GSGSS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAAGGGSGSGSS 349

[Close]

BLAST of CSPI02G00800 vs. NCBI nr
Match: XP_022946035.1 (probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-170
Identity = 317/349 (90.83%), Postives = 333/349 (95.42%), Query Frame = 0


Query: 1   MPMQFSLRSLYTQSHKLTPTLSLPFLSFLRRTMSTSGDIVSPSTTRVGWIGTGVMGCSMC 60
           MP+Q SLRSLYTQSHKL PTLSLPFL +LRRTM+TSGDIVSP+TTRVGWIGTGVMGCSMC
Sbjct: 1   MPLQISLRSLYTQSHKLNPTLSLPFLPYLRRTMATSGDIVSPTTTRVGWIGTGVMGCSMC 60

Query: 61  SHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120
           SHIIKAG+KLTVFNRTISKAQPLLDLGANLA+SPLAVAAQSDVVFSIVGYPSDVRSVLLD
Sbjct: 61  SHIIKAGFKLTVFNRTISKAQPLLDLGANLASSPLAVAAQSDVVFSIVGYPSDVRSVLLD 120

Query: 121 PTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIF 180
           PTSGALAGLRPGGVL+DMTTSEPSLASEIAA+  AKGC A+DAPVSGGDRGAKNA+LAIF
Sbjct: 121 PTSGALAGLRPGGVLVDMTTSEPSLASEIAAATAAKGCKAVDAPVSGGDRGAKNASLAIF 180

Query: 181 AGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGL 240
           AGG+E EV+RL+PLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYA KAGL
Sbjct: 181 AGGNEGEVRRLNPLFSLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAHKAGL 240

Query: 241 DVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300
           DV LFLNAISTGAAGSKSLDLYGSRIL+RDFEPGFFVNHFVKDLGICLKECQNMGLALPG
Sbjct: 241 DVSLFLNAISTGAAGSKSLDLYGSRILQRDFEPGFFVNHFVKDLGICLKECQNMGLALPG 300

Query: 301 LALAQQLYVSLKAYGEGNLGTQALILSLERLNNVSLE-------GSGSS 343
           LALAQQLYVSLKA+GEGNLGTQALILSLERLNNVSL+       GSGSS
Sbjct: 301 LALAQQLYVSLKAHGEGNLGTQALILSLERLNNVSLQSAARGGSGSGSS 349

[Close]

BLAST of CSPI02G00800 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 3.3e-136
Identity = 248/326 (76.07%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 19  PTLSLPFLSFLRRTMST---SGDIVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNR 78
           P+ S+    FLRR+M++   S DI++PS T++GWIGTGVMG SMC H+IKAGY +TVFNR
Sbjct: 9   PSPSVVSSFFLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNR 68

Query: 79  TISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVL 138
           TISKAQ L+D+GAN+A SP +VA QSDVVF+IVGYPSDVR VLLDP SGAL+GLR GGVL
Sbjct: 69  TISKAQTLIDMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVL 128

Query: 139 IDMTTSEPSLASEIAASATAKGCGAIDAPVSGGDRGAKNATLAIFAGGDEDEVQRLSPLF 198
           +DMTTSEPSLA EIA +A+ K C +IDAPVSGGD GAKN  L+IFAGGDE  V+RL PLF
Sbjct: 129 VDMTTSEPSLAEEIAKAASFKNCFSIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLF 188

Query: 199 SLLGKVNYMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAG 258
           SL+GKVN+MG SGKGQFAKLANQITIASTM+GLVEG+IYA KAGLDV  FL AISTGAAG
Sbjct: 189 SLMGKVNFMGTSGKGQFAKLANQITIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAG 248

Query: 259 SKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYG 318
           SKS+DLYG RILKRDF+PGF+VNHFVKDLGICL ECQ MGLALPGLALAQQLY+SLKA+G
Sbjct: 249 SKSIDLYGDRILKRDFDPGFYVNHFVKDLGICLNECQRMGLALPGLALAQQLYLSLKAHG 308

Query: 319 EGNLGTQALILSLERLNNVSLEGSGS 342
           EG+LGTQAL+L+LERLNNVS++ S S
Sbjct: 309 EGDLGTQALLLALERLNNVSVQSSDS 334

[Close]

BLAST of CSPI02G00800 vs. TAIR 10
Match: AT1G71180.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.3e-76
Identity = 149/297 (50.17%), Postives = 200/297 (67.34%), Query Frame = 0


Query: 39  IVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVA 98
           ++ PS TR+GWIG G+MG +M SHII AGY +TV+ R + K + L   GA +A SP  +A
Sbjct: 29  LIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELA 88

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL PGGV +DMT+S+P LA EI A A  + C
Sbjct: 89  EMSDVVFTIVGNFNDVRSLLLG-DDGVLSGLTPGGVTVDMTSSKPGLAREIHAEARRRNC 148

Query: 159 GAIDAPVSGGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQ 218
            A+DAPVSGGD GA+  TL IFAGGD + V+ LSP+   +G V YMGE+G GQ  K+ NQ
Sbjct: 149 WAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQSCKIGNQ 208

Query: 219 ITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVN 278
           I  AS +VGL EG+++A+KAGLD   +L A+  GAAGS  + L+G  I+KRD+    F  
Sbjct: 209 IAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYRATGFAE 268

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +       A+PG AL++QL+  + A G+G LG Q ++  + RLN +S
Sbjct: 269 YMVKDLGMAAE------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSVIRRLNGIS 318

[Close]

BLAST of CSPI02G00800 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-71
Identity = 139/297 (46.80%), Postives = 195/297 (65.66%), Query Frame = 0


Query: 39  IVSPSTTRVGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVA 98
           ++ PS TR+GWIG G+MG +M SHI+ AGY +TV+ R + K + L   G   A SP  + 
Sbjct: 8   LIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELG 67

Query: 99  AQSDVVFSIVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGC 158
             SDVVF+IVG  +DVRS+LL    G L+GL+PGGV +DMT+S+P LA EI A A  + C
Sbjct: 68  EMSDVVFTIVGNSNDVRSLLLG-DDGVLSGLKPGGVTVDMTSSKPGLAREIYAEARRRDC 127

Query: 159 GAIDAPVSGGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGKVNYMGESGKGQFAKLANQ 218
            A+DAPVSGGD GA+   L IFAGGD + V+ L+P+   +G V +MG +G GQ  K+ NQ
Sbjct: 128 WAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQSCKIGNQ 187

Query: 219 ITIASTMVGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVN 278
           I + S M+GL EG+++A+KAGLD   +L A+  GAAGS  + L+G  +  RD++   F  
Sbjct: 188 ICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYKATGFAE 247

Query: 279 HFVKDLGICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALILSLERLNNVS 336
           + VKDLG+  +      +A+PG AL +QL+  + A G+G LG Q ++  + RLN +S
Sbjct: 248 YMVKDLGMAAE----AAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDVIRRLNGLS 299

[Close]

BLAST of CSPI02G00800 vs. TAIR 10
Match: AT1G17650.1 (glyoxylate reductase 2 )

HSP 1 Score: 149.8 bits (377), Expect = 3.9e-36
Identity = 102/322 (31.68%), Postives = 166/322 (51.55%), Query Frame = 0


Query: 17  LTPTLSLPFLSFLRRTMSTSGDIVSPS------TTRVGWIGTGVMGCSMCSHIIKAGYKL 76
           ++P LS P +    R  S S    +PS      T  +G++G G+MG  M  ++IKAG  +
Sbjct: 34  ISPFLSKPQICLAYRVYS-SLQSTTPSTRDELGTVSIGFLGMGIMGSPMAQNLIKAGCDV 93

Query: 77  TVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFSIVGYPSDVRSVLLDPTSGALAGLR 136
           TV+NRT SK  PL+ LGA   +SP  V A  D+ F+++  P     V     +GA+ G+ 
Sbjct: 94  TVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFAMLADPESAIDVACG-KNGAIFGIS 153

Query: 137 PGGVLIDMTTSEPSLASEIAASATAKGCGA--IDAPVSGGDRGAKNATLAIFAGGDEDEV 196
            G   +D++T +  +AS I  S   K  GA  ++APVSG  + A++  L     GD+   
Sbjct: 154 SGKGYVDVSTVD--VASSILISKQIKDTGALFLEAPVSGSKKPAEDGQLIFLTAGDKPLY 213

Query: 197 QRLSPLFSLLGKVN-YMGESGKGQFAKLANQITIASTMVGLVEGMIYAKKAGLDVGLFLN 256
           ++ +P   ++GK   Y+GE G G   KL   + + S M    EG++ ++K GLD  + + 
Sbjct: 214 EKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSMMASFAEGILLSQKVGLDPNVLVE 273

Query: 257 AISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLGICLKECQNMGLALPGLALAQQL 316
            +S GA  +    L G  ++K  +   F + H  KD+ + L   +++  + P  A A +L
Sbjct: 274 VVSQGAINAPMYSLKGPSMIKSVYPTAFPLKHQQKDMRLALGLAESVSQSTPIAAAANEL 333

Query: 317 YVSLKAYGEGNLGTQALILSLE 330
           Y   K+YG  +    A+I +L+
Sbjct: 334 YKVAKSYGLSDEDFSAVIEALK 351

[Close]

BLAST of CSPI02G00800 vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 135.6 bits (340), Expect = 7.7e-32
Identity = 86/280 (30.71%), Postives = 145/280 (51.79%), Query Frame = 0


Query: 47  VGWIGTGVMGCSMCSHIIKAGYKLTVFNRTISKAQPLLDLGANLATSPLAVAAQSDVVFS 106
           VG++G G+MG +M  +++K G+K+TV+NRT+SK   L++ GA++  SP  V  +     +
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 107 IVGYPSDVRSVLLDPTSGALAGLRPGGVLIDMTTSEPSLASEIAASATAKGCGAIDAPVS 166
           ++  P    SV+ D   G L  +  G   IDM+T +   + +I  + T KG   ++ PVS
Sbjct: 63  MLSDPCAALSVVFD-KGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVS 122

Query: 167 GGDRGAKNATLAIFAGGDEDEVQRLSPLFSLLGKVN-YMGESGKGQFAKLANQITIASTM 226
           G  + A++  L I A GD+   +   P F +LGK + Y+G+ G G   KL   + + S M
Sbjct: 123 GSKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMM 182

Query: 227 VGLVEGMIYAKKAGLDVGLFLNAISTGAAGSKSLDLYGSRILKRDFEPGFFVNHFVKDLG 286
               EG++ A K+GL     L+ +  GA  +      G  + K  + P F + H  KD+ 
Sbjct: 183 NAFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMR 242

Query: 287 ICLKECQNMGLALPGLALAQQLYVSLKAYGEGNLGTQALI 326
           + L       +++P  A A + +   ++ G G+L   A+I
Sbjct: 243 LALALGDENAVSMPVAAAANEAFKKARSLGLGDLDFSAVI 281

The following BLAST results are available for this feature:

BLAST of CSPI02G00800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZE1	4.7e-135	76.07	Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis...	[more]
Q949M8	3.2e-75	50.17	Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis...	[more]
Q9C991	3.1e-70	46.80	Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis...	[more]
O34948	2.7e-66	48.03	Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) OX=224308 ...	[more]
O33730	2.1e-42	37.28	Uncharacterized oxidoreductase Sfri_1503 OS=Shewanella frigidimarina (strain NCI...	[more]

BLAST of CSPI02G00800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIQ8	3.3e-184	99.42	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000840 PE=4 SV=1	[more]
A0A1S3C8W8	7.2e-179	96.48	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucumis mel...	[more]
A0A6J1G2N8	7.2e-171	90.83	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita m...	[more]
A0A6J1KJW1	5.2e-169	90.75	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Cucurbita m...	[more]
A0A5D3CJZ2	2.1e-162	97.09	Putative 3-hydroxyisobutyrate dehydrogenase-like 1 OS=Cucumis melo var. makuwa O...	[more]

BLAST of CSPI02G00800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141022.2	6.9e-184	99.42	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucumis sativ...	[more]
XP_008458836.1	1.5e-178	96.48	PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cu...	[more]
XP_038889572.1	2.7e-172	94.10	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Benincasa his...	[more]
XP_023545872.1	7.9e-172	91.69	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita pep...	[more]
XP_022946035.1	1.5e-170	90.83	probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial [Cucurbita mos...	[more]

BLAST of CSPI02G00800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29120.1	3.3e-136	76.07	6-phosphogluconate dehydrogenase family protein	[more]
AT1G71180.1	2.3e-76	50.17	6-phosphogluconate dehydrogenase family protein	[more]
AT1G71170.1	2.2e-71	46.80	6-phosphogluconate dehydrogenase family protein	[more]
AT1G17650.1	3.9e-36	31.68	glyoxylate reductase 2	[more]
AT3G25530.1	7.7e-32	30.71	glyoxylate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013328	6-phosphogluconate dehydrogenase, domain 2	GENE3D	1.10.1040.10		coord: 206..335 e-value: 1.4E-36 score: 127.6
IPR015815	3-hydroxyisobutyrate dehydrogenase-related	PIRSF	PIRSF000103	HIBADH	coord: 41..335 e-value: 9.5E-62 score: 206.7
IPR029154	3-hydroxyisobutyrate dehydrogenase, NAD-binding domain	PFAM	PF14833	NAD_binding_11	coord: 208..325 e-value: 5.3E-32 score: 110.6
IPR006115	6-phosphogluconate dehydrogenase, NADP-binding	PFAM	PF03446	NAD_binding_2	coord: 46..202 e-value: 7.2E-43 score: 146.5
None	No IPR available	GENE3D	3.40.50.720		coord: 40..205 e-value: 7.2E-47 score: 161.3
None	No IPR available	PANTHER	PTHR43060	3-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 1, MITOCHONDRIAL-RELATED	coord: 39..334
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 45..203
IPR008927	6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily	SUPERFAMILY	48179	6-phosphogluconate dehydrogenase C-terminal domain-like	coord: 206..335

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G00800.1	CSPI02G00800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0050661	NADP binding
molecular_function	GO:0016491	oxidoreductase activity