CSPI02G00340 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI02G00340
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionE3 ubiquitin ligase
LocationChr2: 208380 .. 211777 (-)
RNA-Seq ExpressionCSPI02G00340
SyntenyCSPI02G00340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAATTCAAAAAATGAATAAATTTTTGGACTGATTCTTGTTGATGGTGGTGTTTTCTAAGGCAATGACATCAGTGGCGATTGCCGTTAACGGCGTCCGCGGCGGCAAAGGCGGTGGAGGAAGCCGCCGCGCTGTCAGATGGGCCGTCGAGAATCTCTTGCCGACCGCCGATCGTTTCATATTGGTTCACGTTATGCCCAAAATCACCTCCATCCCAACCCCAAGTATTTTTTTTTCTCTAAAACTGTTTGTTTGGTTGCTGGGAAACACTGGGAAAATAAATCTTGACAACAACTTCTTAATCTTTTTGTTATATAGTGGGGGATCTTGTGGCTGTCTCAGAACTTGATGAAGATGTGGTAGCTCTTTATGTTCATGATGTGAAACAGAAATATGAACAAGTTTTTGTCCCTTTTAAGAAGTTATGTAAAAGAGAAAAGGTAGTTTCAACTTCATTTTTTAAAGTTTAGCTCCAAATTTGTAGTAAACTGAAAATGGGCATTTTTTCTTCAGTTTCTTATTTTGGTAGAAACTTTGATTTTGGAGGATGATAATCCTGCCACTGCACTTTTGAGATATGCATCTGAATCTGGGATTAAAAGCTTGGTTTTGGGTTCTTGTTTTAGAACTTGCATAGCAAGGTAATTTGGTAGATTTTATGTTATTAACTTATCATTCAGCCTATGACATTTTGTGCTAATGTTTGTGTCAAAATTCAAATTCATTCATGAAATGATGATTTGATCTTCAACATTTTGATACTAACTTTTGGAAAATTTATCTGAAGGAAGCTAAAAGGTGATTCAGTACCCTCTGCTATTATGAGAACTGCTTCTAGTTCTTTTGATATTTATGTTAAATATAAGAGAAGAGTTATCACAAGAAAGGCTAGTACTGCTCCATCTACTGGTAATTTGTTGAAACCGTTTGCTTCTCTTGTTAGTTTTTTCTAATACAAGATGTTAAGAAATCGACAATCAAAGAATAAACAGAAAGTGAAAACAATAAATAATTGATACAAATTTACATGGTTTACTAACAGTAAGTTAGTTATGTCCACAAATAGAGGGAGTGTGTTAGATATCATATTAAATTTGTCTTTGTCCATTGAGTTTGGTTGATTGGTAATTTTAGAGAGTGAATGATGTAAATAAGATAAATATGAATACAACTTATATTAATTTTCAATGATGTGTGTAATTGTTTTTGCAGAAACTGACTCGAGGCAGTGGATGCTTGGTGACACTGATTACTACAAGGGTTCCAGTGCTGATAGTGAAAAATCATTGGGAACTGATATGTCTTCGTCTTACTTGTCGATTGTTCATCAAAGGGATGACAGCATCGGAGTTGACTCTACTGAGCAACTGCGTACTCTTACTGAGGAGGTTTTTGTTGTCTTTTTGTTTTATTGTTTCAAGCTTTGCTTCCATCTCGGTTTTGTTTTCTCGATGGAATTTTGTCACAATTTCGAGGTGATGCTACAGTGAATCTTTAATAAAGGTCCTAAATTGCAGGAAGATATGCAGTCTGAAGTTGAATCTCTGCAGCTAGAACTAGAGACCACTGTTTCCTTGTACAAACAAGCTTGTGAAGAACTAGTACGCACTCAGAAGAAGGTGAGAGAGCTCAATTTTGGTGATTTAATTTGAATTTTAACCCAACTATTGGCTTTACAAGAATTAAAAAGAAACAATGTAAATGCCTATTTATTCTTCAATTTTCAGGTTCAGTCACTTACACAAGAATACCTTGAAGAATCAAGAAAAGTGACTGATGCTGTGGAAAGGGAACAAGCTTTGAGGAAAGTTGCTGCCAAGGAGAAAGCAAAGCACTTGGAAGCCATTAAAGAGCTGGAGGAGGCTAAAGACTTGCTGGCTAAAGAAGCTTATGAAAGACAGTTAGCTGAGCTTGATGCCTTAAAGGAGTCCGTGGAGAAGCAGAAAATTATCGATACTCTTCTGACTAATGATAGGAGATACAGAAGATATACCACTGCTGAAATAGAGGCTGCAACTAATTTCTTCAATGAAGTTAATGTGATCGGCGAGGGAGGATATGGGAAAGTCTATAAAAGCAGTCTTGATCATACCCCTGTAGCCATTAAGGTTTTTCAACACGACATATTCGAGAAAAAAGACGAGTTTCTAAAAGAGGTACCGTTTGTTACAAAAATTTTCTTACTGACATTCATGTTTTTTTAATCCGATTAATGATCCTCATTCCATCACCTGAACTTATCTCTGCAGGTTGAAATTCTTAGCCAGATACGACATCCACACGTGGTTTTACTGCTTGGAGCTTGTCCCGAGCGAGGTTGCTTGATCTATGAATACATGGAAAATGGAAGCTTAGATGACCATATCTTGCTCCGGAATGGAAAAGCTCCCCTTCCTTGGTCTACTAGATTTAGAATAGTTTTTCAAGTAGCTTCTGGGCTTGCATTTTTACACAATTCAAAGCCAGAGCCTATTATTCATAGAGATCTCAAACCGGGTAATATCTTACTTGACAGAAACTTCGTAAGCAAAATCTCAGATGTTGGAATGGCTAAGATCATTGGCGATATCGTGCCAGACAATGTCACGGAGTACCAGAACACCGTTCTTGCTGGTACTCTACATTACATGGATCCTGAATATCAGAGAACTGGCACACTCCGGCCAAAGTCGGATACTTACGCACTTGGAGTAACAATTCTGCAGTTATTGACTGGGCGACAGCCACACGGTCTTCTTCTCGCTATTGAAAATTCCATTGCAAGCGCTTCCCTTGCAGATATTCTAGACAAGTCAATTAGTAACTGGCCTCTAGCTAAAGCTGAAGAGCTAGCTCGACTTGCATTGAAGTGCTTGAAACTTAGATGCAGGGATAGGCCAGATCTTGAATCCGAAGTTCTTCCAATCCTTAAAAGACTTGTTGATTTTGCAGACACTTTTCAAAATGAAGACAATGGTTTTGGCAACCCCCCCAGCCACTACTTCTGCCCAATCCTCCAGGTGAACTTAAAACACACACTACACACACCATCATCAGTTCTTCAGAAGCTTGAGTATAAACTTGTTTTGTTGTATTTTATCTTTTGAACACAGGAAGTCATGGAGGATCCTTATATAGCAGCTGATGGCTTTAGTTATGAGTATGTTGCCATTAAAGCATGGCTTGAGAAACACGACGTATCGCCGGCGACAAAGCTTAAGCTCCGGCATTCTTTTTTTATTCCAAACTACACTCTACGTTCTGCCATACGAGAGTGGAGGTCAAGAGTGACATTTTCGTCTTCCTAAGTTGGACAATTTGATTGTAATTTCTCAAGCACTCGTATGGTTGTTCCTTTCGTCTTTGTTAATATTTGTATAGTGTATAGTTACAGCGTAGTCTCAGGAATGAATTGAGAG

mRNA sequence

GTAAATTCAAAAAATGAATAAATTTTTGGACTGATTCTTGTTGATGGTGGTGTTTTCTAAGGCAATGACATCAGTGGCGATTGCCGTTAACGGCGTCCGCGGCGGCAAAGGCGGTGGAGGAAGCCGCCGCGCTGTCAGATGGGCCGTCGAGAATCTCTTGCCGACCGCCGATCGTTTCATATTGGTTCACGTTATGCCCAAAATCACCTCCATCCCAACCCCAATGGGGGATCTTGTGGCTGTCTCAGAACTTGATGAAGATGTGGTAGCTCTTTATGTTCATGATGTGAAACAGAAATATGAACAAGTTTTTGTCCCTTTTAAGAAGTTATGTAAAAGAGAAAAGTTTCTTATTTTGGTAGAAACTTTGATTTTGGAGGATGATAATCCTGCCACTGCACTTTTGAGATATGCATCTGAATCTGGGATTAAAAGCTTGGTTTTGGGTTCTTGTTTTAGAACTTGCATAGCAAGGAAGCTAAAAGGTGATTCAGTACCCTCTGCTATTATGAGAACTGCTTCTAGTTCTTTTGATATTTATGTTAAATATAAGAGAAGAGTTATCACAAGAAAGGCTAGTACTGCTCCATCTACTGAAACTGACTCGAGGCAGTGGATGCTTGGTGACACTGATTACTACAAGGGTTCCAGTGCTGATAGTGAAAAATCATTGGGAACTGATATGTCTTCGTCTTACTTGTCGATTGTTCATCAAAGGGATGACAGCATCGGAGTTGACTCTACTGAGCAACTGCGTACTCTTACTGAGGAGGAAGATATGCAGTCTGAAGTTGAATCTCTGCAGCTAGAACTAGAGACCACTGTTTCCTTGTACAAACAAGCTTGTGAAGAACTAGTACGCACTCAGAAGAAGGTTCAGTCACTTACACAAGAATACCTTGAAGAATCAAGAAAAGTGACTGATGCTGTGGAAAGGGAACAAGCTTTGAGGAAAGTTGCTGCCAAGGAGAAAGCAAAGCACTTGGAAGCCATTAAAGAGCTGGAGGAGGCTAAAGACTTGCTGGCTAAAGAAGCTTATGAAAGACAGTTAGCTGAGCTTGATGCCTTAAAGGAGTCCGTGGAGAAGCAGAAAATTATCGATACTCTTCTGACTAATGATAGGAGATACAGAAGATATACCACTGCTGAAATAGAGGCTGCAACTAATTTCTTCAATGAAGTTAATGTGATCGGCGAGGGAGGATATGGGAAAGTCTATAAAAGCAGTCTTGATCATACCCCTGTAGCCATTAAGGTTTTTCAACACGACATATTCGAGAAAAAAGACGAGTTTCTAAAAGAGGTTGAAATTCTTAGCCAGATACGACATCCACACGTGGTTTTACTGCTTGGAGCTTGTCCCGAGCGAGGTTGCTTGATCTATGAATACATGGAAAATGGAAGCTTAGATGACCATATCTTGCTCCGGAATGGAAAAGCTCCCCTTCCTTGGTCTACTAGATTTAGAATAGTTTTTCAAGTAGCTTCTGGGCTTGCATTTTTACACAATTCAAAGCCAGAGCCTATTATTCATAGAGATCTCAAACCGGGTAATATCTTACTTGACAGAAACTTCGTAAGCAAAATCTCAGATGTTGGAATGGCTAAGATCATTGGCGATATCGTGCCAGACAATGTCACGGAGTACCAGAACACCGTTCTTGCTGGTACTCTACATTACATGGATCCTGAATATCAGAGAACTGGCACACTCCGGCCAAAGTCGGATACTTACGCACTTGGAGTAACAATTCTGCAGTTATTGACTGGGCGACAGCCACACGGTCTTCTTCTCGCTATTGAAAATTCCATTGCAAGCGCTTCCCTTGCAGATATTCTAGACAAGTCAATTAGTAACTGGCCTCTAGCTAAAGCTGAAGAGCTAGCTCGACTTGCATTGAAGTGCTTGAAACTTAGATGCAGGGATAGGCCAGATCTTGAATCCGAAGTTCTTCCAATCCTTAAAAGACTTGTTGATTTTGCAGACACTTTTCAAAATGAAGACAATGGTTTTGGCAACCCCCCCAGCCACTACTTCTGCCCAATCCTCCAGGAAGTCATGGAGGATCCTTATATAGCAGCTGATGGCTTTAGTTATGAGTATGTTGCCATTAAAGCATGGCTTGAGAAACACGACGTATCGCCGGCGACAAAGCTTAAGCTCCGGCATTCTTTTTTTATTCCAAACTACACTCTACGTTCTGCCATACGAGAGTGGAGGTCAAGAGTGACATTTTCGTCTTCCTAAGTTGGACAATTTGATTGTAATTTCTCAAGCACTCGTATGGTTGTTCCTTTCGTCTTTGTTAATATTTGTATAGTGTATAGTTACAGCGTAGTCTCAGGAATGAATTGAGAG

Coding sequence (CDS)

ATGGTGGTGTTTTCTAAGGCAATGACATCAGTGGCGATTGCCGTTAACGGCGTCCGCGGCGGCAAAGGCGGTGGAGGAAGCCGCCGCGCTGTCAGATGGGCCGTCGAGAATCTCTTGCCGACCGCCGATCGTTTCATATTGGTTCACGTTATGCCCAAAATCACCTCCATCCCAACCCCAATGGGGGATCTTGTGGCTGTCTCAGAACTTGATGAAGATGTGGTAGCTCTTTATGTTCATGATGTGAAACAGAAATATGAACAAGTTTTTGTCCCTTTTAAGAAGTTATGTAAAAGAGAAAAGTTTCTTATTTTGGTAGAAACTTTGATTTTGGAGGATGATAATCCTGCCACTGCACTTTTGAGATATGCATCTGAATCTGGGATTAAAAGCTTGGTTTTGGGTTCTTGTTTTAGAACTTGCATAGCAAGGAAGCTAAAAGGTGATTCAGTACCCTCTGCTATTATGAGAACTGCTTCTAGTTCTTTTGATATTTATGTTAAATATAAGAGAAGAGTTATCACAAGAAAGGCTAGTACTGCTCCATCTACTGAAACTGACTCGAGGCAGTGGATGCTTGGTGACACTGATTACTACAAGGGTTCCAGTGCTGATAGTGAAAAATCATTGGGAACTGATATGTCTTCGTCTTACTTGTCGATTGTTCATCAAAGGGATGACAGCATCGGAGTTGACTCTACTGAGCAACTGCGTACTCTTACTGAGGAGGAAGATATGCAGTCTGAAGTTGAATCTCTGCAGCTAGAACTAGAGACCACTGTTTCCTTGTACAAACAAGCTTGTGAAGAACTAGTACGCACTCAGAAGAAGGTTCAGTCACTTACACAAGAATACCTTGAAGAATCAAGAAAAGTGACTGATGCTGTGGAAAGGGAACAAGCTTTGAGGAAAGTTGCTGCCAAGGAGAAAGCAAAGCACTTGGAAGCCATTAAAGAGCTGGAGGAGGCTAAAGACTTGCTGGCTAAAGAAGCTTATGAAAGACAGTTAGCTGAGCTTGATGCCTTAAAGGAGTCCGTGGAGAAGCAGAAAATTATCGATACTCTTCTGACTAATGATAGGAGATACAGAAGATATACCACTGCTGAAATAGAGGCTGCAACTAATTTCTTCAATGAAGTTAATGTGATCGGCGAGGGAGGATATGGGAAAGTCTATAAAAGCAGTCTTGATCATACCCCTGTAGCCATTAAGGTTTTTCAACACGACATATTCGAGAAAAAAGACGAGTTTCTAAAAGAGGTTGAAATTCTTAGCCAGATACGACATCCACACGTGGTTTTACTGCTTGGAGCTTGTCCCGAGCGAGGTTGCTTGATCTATGAATACATGGAAAATGGAAGCTTAGATGACCATATCTTGCTCCGGAATGGAAAAGCTCCCCTTCCTTGGTCTACTAGATTTAGAATAGTTTTTCAAGTAGCTTCTGGGCTTGCATTTTTACACAATTCAAAGCCAGAGCCTATTATTCATAGAGATCTCAAACCGGGTAATATCTTACTTGACAGAAACTTCGTAAGCAAAATCTCAGATGTTGGAATGGCTAAGATCATTGGCGATATCGTGCCAGACAATGTCACGGAGTACCAGAACACCGTTCTTGCTGGTACTCTACATTACATGGATCCTGAATATCAGAGAACTGGCACACTCCGGCCAAAGTCGGATACTTACGCACTTGGAGTAACAATTCTGCAGTTATTGACTGGGCGACAGCCACACGGTCTTCTTCTCGCTATTGAAAATTCCATTGCAAGCGCTTCCCTTGCAGATATTCTAGACAAGTCAATTAGTAACTGGCCTCTAGCTAAAGCTGAAGAGCTAGCTCGACTTGCATTGAAGTGCTTGAAACTTAGATGCAGGGATAGGCCAGATCTTGAATCCGAAGTTCTTCCAATCCTTAAAAGACTTGTTGATTTTGCAGACACTTTTCAAAATGAAGACAATGGTTTTGGCAACCCCCCCAGCCACTACTTCTGCCCAATCCTCCAGGAAGTCATGGAGGATCCTTATATAGCAGCTGATGGCTTTAGTTATGAGTATGTTGCCATTAAAGCATGGCTTGAGAAACACGACGTATCGCCGGCGACAAAGCTTAAGCTCCGGCATTCTTTTTTTATTCCAAACTACACTCTACGTTCTGCCATACGAGAGTGGAGGTCAAGAGTGACATTTTCGTCTTCCTAA

Protein sequence

MVVFSKAMTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAVSELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASESGIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETDSRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLRTLTEEEDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIREWRSRVTFSSS*
Homology
BLAST of CSPI02G00340 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 5.6e-202
Identity = 392/778 (50.39%), Postives = 523/778 (67.22%), Query Frame = 0

Query: 11  VAIAVNGVRGGK-GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP--------- 70
           VA+AV G+ G K GG GSRRAVRWAV+NLLP AD+F+++HV+P ITSIPTP         
Sbjct: 24  VAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHVIPTITSIPTPNILILMFTR 83

Query: 71  -----MGDLVAVSELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFL-ILVETLILEDD 130
                 GD + V E++E VV +YV DVK++YE VFVPF K+CK  +       +   +  
Sbjct: 84  MWVVTAGDRLPVEEVEESVVEMYVRDVKKEYETVFVPFLKMCKSTRSTKRYFRSRRTKGT 143

Query: 131 NPATALLRYASESGIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVI 190
                +LRYA E+    +V      T     L  +  P      A+++ D    +     
Sbjct: 144 GVPLTVLRYAPETCEVYIVCKDRITTKSMDPLI-NREPCTSPHAAATAHDFLRDWAASFH 203

Query: 191 TRKASTAP----STETDSRQWMLG----------------DTDYYKGSSADS----EKSL 250
           T ++ T P    STE  +R+                         K SSA +     +  
Sbjct: 204 TLRSPTLPDPRQSTEAGTRRSASARELRFEALSLTCNKPKTPQSSKASSATTPEIFRRRR 263

Query: 251 GTDM---------------SSSYLSIVHQRDDSIGVDSTEQLRTLTEEEDMQSEVESLQL 310
           G+D+                S+  +IV +  DS    S  +    +++ +++ EVE L+ 
Sbjct: 264 GSDIPQLNYSDFDKTCTKPQSNVENIVSEHRDS--DRSPPETSRKSKKVEIEEEVERLKN 323

Query: 311 ELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAAKEKAKHLE 370
           EL++TV  YKQACEEL  TQ KV+ L+ EYL ES++V +AVE+E+  R  AA EK ++++
Sbjct: 324 ELQSTVFKYKQACEELFSTQNKVKMLSTEYLNESKRVNNAVEKEELQRNTAALEKERYMK 383

Query: 371 AIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATN 430
           A+KE+E AK LLA+E  +RQ+AE++AL+  +EK+K+ID LL  D RYR+YT  EI  AT 
Sbjct: 384 AVKEVETAKALLAREFCQRQIAEVNALRTYLEKKKVIDQLLGTDHRYRKYTIEEIVTATE 443

Query: 431 FFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLL 490
            F+   VIGEGGYGKVY+ SLD TP A+KV + D  EKK EFLKEVE+LSQ+RHPHVVLL
Sbjct: 444 GFSPEKVIGEGGYGKVYQCSLDSTPAAVKVVRLDTPEKKQEFLKEVEVLSQLRHPHVVLL 503

Query: 491 LGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPI 550
           LGACPE GCL+YEY+ENGSL+++I  R  K PLPW  RFR++F+VA GLAFLH+SKPEPI
Sbjct: 504 LGACPENGCLVYEYLENGSLEEYIFHRKNKPPLPWFIRFRVIFEVACGLAFLHSSKPEPI 563

Query: 551 IHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTLHYMDPEYQRTG 610
           +HRDLKPGNILL+RN+VSKI+DVG+AK++ D+ PDNVT Y+N+VLAGTLHY+DPEY RTG
Sbjct: 564 VHRDLKPGNILLNRNYVSKIADVGLAKLVTDVAPDNVTMYRNSVLAGTLHYIDPEYHRTG 623

Query: 611 TLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISNWPLAKAEELA 670
           T+RPKSD YA G+ ILQLLT R P G++ A+EN++   +L ++LDKS+++WPLA+ EELA
Sbjct: 624 TIRPKSDLYAFGIIILQLLTARNPSGIVPAVENAVKKGTLTEMLDKSVTDWPLAETEELA 683

Query: 671 RLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHYFCPILQEVMED 730
           R+ LKC + RCRDRPDL+SEV+P+LKRLV+ A++   ++      PSHYFCPIL+E+ME+
Sbjct: 684 RIGLKCAEFRCRDRPDLKSEVIPVLKRLVETANSKVKKEGSNLRAPSHYFCPILREIMEE 743

Query: 731 PYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIREWRSRVTFSS 734
           P IAADGF+YE  AI AWLEKH++SP T+ KL H    PN+TLRSAIR+W+SRV FS+
Sbjct: 744 PEIAADGFTYERKAILAWLEKHNISPVTRQKLDHFKLTPNHTLRSAIRDWKSRVRFSN 798

BLAST of CSPI02G00340 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 5.9e-135
Identity = 312/828 (37.68%), Postives = 450/828 (54.35%), Query Frame = 0

Query: 10  SVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDLVAVS 69
           SVA+A+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 70  ELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASESG 129
           EL EDVV+ Y  ++     ++  P+KK+ +R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 130 IKSLVLGSCFRTCIARKLKGDS-----VPS------------AIMRTASSSFDIYVKYKR 189
           +  LV+G   R   +RK+   S     VP             A +R + S     ++++R
Sbjct: 143 VTKLVIGMSLRGFFSRKIDMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIRFER 202

Query: 190 RVITRKASTAPSTETDSRQWMLGDTD-------------YYKGSSADSE---KSLGTDM- 249
              T  ++ +P    + + ++   ++             +  GS+A ++    S GTD  
Sbjct: 203 SSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGTDQE 262

Query: 250 ---SSSYLSIVH-------QRDDS------IGVDS------TEQLRTLTEEEDMQS---- 309
              +   + IVH        +D+S      +G ++      T + R   +  +M+S    
Sbjct: 263 EVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSSSSS 322

Query: 310 -----------------------------------------------EVESLQLELETTV 369
                                                          E+E L+ EL+   
Sbjct: 323 NNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELKHVQ 382

Query: 370 SLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAAKEKAKHLEAIKELE 429
            +Y  A  E V   KK+  L Q   EES K+ +  E+E+  +  A+KEK ++ EA+KE E
Sbjct: 383 EMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMKEAE 442

Query: 430 EAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVN 489
           + K+L+ KEA  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI AAT+ F E  
Sbjct: 443 KVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFAENL 502

Query: 490 VIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPE 549
            IG G YG VYK +L HT  A+KV      +   +F +E+EILS+IRHPH+VLLLGACPE
Sbjct: 503 KIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGACPE 562

Query: 550 RGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLK 609
           RGCL+YEYM+NGSLDD ++L N   P+PW  RFRI  +VAS L FLH SKP PIIHRDLK
Sbjct: 563 RGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHRDLK 622

Query: 610 PGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKS 669
           PGNILLD NFVSK+ DVG++ ++      + T ++ T   GTL Y+DPEYQRTG + PKS
Sbjct: 623 PGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTGIISPKS 682

Query: 670 DTYALGVTILQLLTGRQPHGLLLAIENSIA-SASLADILDKSISNWPLAKAEELARLALK 728
           D Y+LGV ILQL+T +    +   +E +I   A    ILDK   +WP++   ELA L L 
Sbjct: 683 DVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTRELAALGLC 742

BLAST of CSPI02G00340 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 447.6 bits (1150), Expect = 2.7e-124
Identity = 293/809 (36.22%), Postives = 430/809 (53.15%), Query Frame = 0

Query: 23  GGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGDLVAVSELDEDVVALYVHD 82
           G   S+  V WA+E      +  F L+H+ P ITS+PTPMG+ + +SE+ +DVV  Y  +
Sbjct: 28  GSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQE 87

Query: 83  VKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASESGIKSLVLGSCFRTC 142
           +  + E++  P+ KL  R K  + VE L++E DN A A+    +   I  +V+G   R+ 
Sbjct: 88  ILWQSEEMLKPYTKLFVRRK--VAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSF 147

Query: 143 IARKLKGDSVPSAIM----------------------------------RTASSS----- 202
            +RK    SV SA+M                                  RT SSS     
Sbjct: 148 FSRKADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSSSGSSGP 207

Query: 203 ------------------FDIYVKYKRR--VITRKAS----------------------- 262
                               + V+  +    I  +AS                       
Sbjct: 208 TSDSSDVMSSAHDSQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLDAEEA 267

Query: 263 ------TAPSTETDSRQWMLGDTDYYKGSSADSEKS-------LGTDMSSSYLSI--VHQ 322
                    ST+T SR W     DY +   A S  S        GT  S S + +   H 
Sbjct: 268 RDVSSINRSSTDTTSR-WTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHS 327

Query: 323 RDDSIGVDSTEQL--RTLTEEE-DMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSL 382
           R      + ++ L  ++ T+ + ++  EVE L+ EL     +Y  A  E     +K+  L
Sbjct: 328 RASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGEL 387

Query: 383 TQEYLEESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDA 442
            Q  LEE+ K+ +   +E   R++A KEK    +A ++ E  ++   +E  +R+ AE  +
Sbjct: 388 NQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKS 447

Query: 443 LKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPV 502
            +++ EK+K+  TL +   +Y+ +   EI AAT+ F+E   IG G YG VYK +L HT  
Sbjct: 448 ARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTA 507

Query: 503 AIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILL 562
            +KV Q    +   +F +E+EILS+IRHPH+VLLLGACPE+G L+YEYMENGSL+D +  
Sbjct: 508 VVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQ 567

Query: 563 RNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMA 622
            N   PLPW  RFRI ++VA+ L FLH SKP+PIIHRDLKP NILLD NFVSK+ DVG++
Sbjct: 568 VNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLS 627

Query: 623 KIIG-DIVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPH 682
            ++  D +    T Y+ T   GTL Y+DPEYQRTG +  KSD Y+ G+ +LQLLT +   
Sbjct: 628 TMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAI 687

Query: 683 GLLLAIENSI-ASASLADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPI 728
            L   +E+++ ++     ILD+   NWP+ +  ELA LAL C +LR +DRPDL+ ++LP 
Sbjct: 688 ALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPA 747

BLAST of CSPI02G00340 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 5.0e-118
Identity = 285/813 (35.06%), Postives = 421/813 (51.78%), Query Frame = 0

Query: 5   SKAMTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGD 64
           S+ MT VA+A++      G   S+  ++WA+       +  F L+H+ PKIT++PT  G+
Sbjct: 29  SEPMT-VALAIS------GSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGN 88

Query: 65  LVAVSELDEDVVALYVHDVKQKYEQVFV-PFKKLCKREKFLI------------LVETLI 124
           +V++SE  E+V A Y   V Q+ ++  + PFKK+C+R+K  I             VE  +
Sbjct: 89  IVSISEELEEVAAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQV 148

Query: 125 LEDDNPATALLRYASESGIKSLVLGSCFRTCIARKL------------------------ 184
           LE ++ A A+ +  ++  I +L++G   +   +R                          
Sbjct: 149 LESNSVAVAITKEVNQHLISNLIIGRSSQAASSRNYDITASISASVSNLCTVYVVSNGGV 208

Query: 185 -----------------------------KGDSVPSAIMRTASSSFDIYVKYKRR----V 244
                                         G    S +M  A  S    +  KR      
Sbjct: 209 HILAKDTSDTERNDTSIESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSNKRMQNLPT 268

Query: 245 ITRKAST---APSTETDSRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIG 304
           I R  S      STE+D  +    D        +  E S     +  +     ++D    
Sbjct: 269 IVRGVSVPMETSSTESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDERKDAMSS 328

Query: 305 VDSTEQLRTL--------TEEEDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLT 364
           + S  +   +        T+ +D  +E+  L+ EL     +Y  A  E +   +K+  L 
Sbjct: 329 MSSNFEYGNVVTPLGHYFTDNQDTLNEISKLRAELRHAHEMYAVAQVETLDASRKLNEL- 388

Query: 365 QEYLEESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDAL 424
                   K  +    E   + +A KE  K         E K    +EA +R+ AE+ A 
Sbjct: 389 --------KFEELTLLEHETKGIAKKETEKF--------EQKRREEREAAQRREAEMKAT 448

Query: 425 KESVEKQKIIDTLLTNDR-RYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPV 484
            E+ EK+K+ ++ L   + +Y+ +T  EI  AT+ F+E   IG G YG VYK +L HT  
Sbjct: 449 HEAKEKEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIA 508

Query: 485 AIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILL 544
           A+KV          +F +E+EILS+IRHPH+VLLLGACP+ G L+YEYMENGSL+D +  
Sbjct: 509 AVKVLHSAESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQ 568

Query: 545 RNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMA 604
            N   P+PW  R RI ++VAS L FLH SKP PIIHRDLKP NILL+ NFVSK+ DVG++
Sbjct: 569 VNDSQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLS 628

Query: 605 KII--GDIVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQP 664
            +I   D +    T Y+ T   GTL Y+DPEYQRTG + PKSD YA G+ ILQLLTG+Q 
Sbjct: 629 TMIQAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQA 688

Query: 665 HGLLLAIENSIASAS---LADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEV 724
             L   +E ++ + +   L  ILD+   NWP+ +  +LA LAL+C +LR +DRPDLE ++
Sbjct: 689 MALTYTVETAMENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQI 748

Query: 725 LPILKRLVDFADTFQNEDNGF-GNPPSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLE 729
           LP+L+ L   AD  +N  +     PPSH+FCP+L++VM++P IAADG++Y+  AI+ W+E
Sbjct: 749 LPVLESLKKVADKARNSLSAAPSQPPSHFFCPLLKDVMKEPCIAADGYTYDRRAIEEWME 808

BLAST of CSPI02G00340 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 388.7 bits (997), Expect = 1.5e-106
Identity = 226/565 (40.00%), Postives = 340/565 (60.18%), Query Frame = 0

Query: 181 APSTETDSRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLRTL 240
           A S + D  + +L    +Y+ SS+      G+D+SS   S   Q +++    +     + 
Sbjct: 235 AGSLDVDESK-LLNQKGFYRTSSSGIGYG-GSDISSWRSS---QMEEASSSSTYSDPTSS 294

Query: 241 TEEEDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQ 300
           + +     E+E L++EL     +Y  A  E++   KK+Q L Q   EE+ ++ +   RE+
Sbjct: 295 SSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNLTIREE 354

Query: 301 ALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTL---LT 360
              +V   E+ +  +A  E E  ++ + +E  ER  AE  A +   EKQ++ D L     
Sbjct: 355 EADEVVEMERERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDALEGGPL 414

Query: 361 NDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEF 420
             ++Y ++   EI  AT+ F++   IG GGYG VY+ +L HT VA+KV   D      +F
Sbjct: 415 QRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQF 474

Query: 421 LKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKA------PLPWS 480
            +E+EILS+IRHPH++LLLGACPERG L+YEYM NGSL++ ++ R          PL W 
Sbjct: 475 HQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWF 534

Query: 481 TRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDN 540
            RFRI +++AS L FLH ++P PI+HRDLKP NILLDRN VSKI DVG++K++       
Sbjct: 535 ERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHA 594

Query: 541 VTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSI- 600
            T +  T   GT  Y+DPEYQRTG + P+SD YA G+ +LQL+T R   GL  +IE ++ 
Sbjct: 595 STVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSIEKALR 654

Query: 601 -ASASLADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADT 660
             +    +ILDK+  +WP+ +A+E+  + L+C ++R RDRPDL  E+LP+L+RL + A  
Sbjct: 655 DQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLKEVASI 714

Query: 661 FQN--EDN---GFGNPPSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATK 720
            +N   DN      N P+H++CPI ++VME+P +A+DG++YE  AIK WL+K+  SP T 
Sbjct: 715 ARNMFADNLIDHHHNAPTHFYCPITKDVMENPCVASDGYTYEKRAIKEWLQKNHKSPMTD 774

Query: 721 LKLRHSFFIPNYTLRSAIREWRSRV 730
           L       +PN++L SAI+EWRS++
Sbjct: 775 LPFPSDSLLPNHSLLSAIKEWRSQL 794

BLAST of CSPI02G00340 vs. ExPASy TrEMBL
Match: A0A0A0LKM6 (E3 ubiquitin ligase OS=Cucumis sativus OX=3659 GN=Csa_2G000350 PE=4 SV=1)

HSP 1 Score: 1405.2 bits (3636), Expect = 0.0e+00
Identity = 725/727 (99.72%), Postives = 725/727 (99.72%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD
Sbjct: 121 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLRTLTEEEDMQ 247
           SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLRTLTEEEDMQ
Sbjct: 181 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLRTLTEEEDMQ 240

Query: 248 SEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAA 307
           SEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAA
Sbjct: 241 SEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAA 300

Query: 308 KEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTT 367
           KEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTT
Sbjct: 301 KEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTT 360

Query: 368 AEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQI 427
           AEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQI
Sbjct: 361 AEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQI 420

Query: 428 RHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFL 487
           RHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFL
Sbjct: 421 RHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFL 480

Query: 488 HNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTLHYM 547
           HNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVT YQNTVLAGTLHYM
Sbjct: 481 HNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTAYQNTVLAGTLHYM 540

Query: 548 DPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISNWP 607
           DPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISNWP
Sbjct: 541 DPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISNWP 600

Query: 608 LAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHYFCP 667
           LAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHYFCP
Sbjct: 601 LAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHYFCP 660

Query: 668 ILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIREWRS 727
           ILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIREWRS
Sbjct: 661 ILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIREWRS 720

Query: 728 RVTFSSS 735
           RVTFSSS
Sbjct: 721 RVTFSSS 727

BLAST of CSPI02G00340 vs. ExPASy TrEMBL
Match: A0A1S3C9U0 (E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103498614 PE=4 SV=1)

HSP 1 Score: 1322.0 bits (3420), Expect = 0.0e+00
Identity = 682/730 (93.42%), Postives = 702/730 (96.16%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           MTSVA+AVNGVRGGKGGGGSRRAVRWAVENLLPTADRF+LVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFVLVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQVFVPFKKLC+REKFL+LVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIK LVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQ---LRTLTEEE 247
           SRQWMLGDTDYY GSSA S KSLGT +S S LSI HQR DSIGVDSTEQ   L TLTEEE
Sbjct: 181 SRQWMLGDTDYYMGSSAVSAKSLGT-LSLSSLSISHQRGDSIGVDSTEQLNSLHTLTEEE 240

Query: 248 DMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRK 307
           DMQ+EVESLQLELETTVSLYKQACEELV TQKKVQSLT EYLEESRKVT A+ERE++LRK
Sbjct: 241 DMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYLEESRKVTAALEREESLRK 300

Query: 308 VAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRR 367
            AAKEKAKHLEAIKELEEAKDLLAKEAYERQLAEL+A+KESVEK+KI+DTLLTNDRRYRR
Sbjct: 301 AAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESVEKEKIVDTLLTNDRRYRR 360

Query: 368 YTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEIL 427
           YTTAEIEAATNFFNEVNVIGEGGYGKVYK SLDHTPVAIKV QHDI+EKKDEFLKEVEIL
Sbjct: 361 YTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVIQHDIYEKKDEFLKEVEIL 420

Query: 428 SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGL 487
           SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHI LRNGKAPLPWSTRFRIVFQVASGL
Sbjct: 421 SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKAPLPWSTRFRIVFQVASGL 480

Query: 488 AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTL 547
           AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEY+NTVLAGTL
Sbjct: 481 AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTVLAGTL 540

Query: 548 HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS 607
           HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS
Sbjct: 541 HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS 600

Query: 608 NWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHY 667
           +WPLAKAEELARLALKCLKLRCRDRPDLESEVLP+LKRLVDFADTFQ ED GFGNPPSHY
Sbjct: 601 DWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDFADTFQKEDKGFGNPPSHY 660

Query: 668 FCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIRE 727
           FCPILQEVMEDPYIAADGF+YEYVAIKAWLEKHDVSP TKLKL+HSF  PN+TLRSAIRE
Sbjct: 661 FCPILQEVMEDPYIAADGFTYEYVAIKAWLEKHDVSPVTKLKLQHSFLXPNHTLRSAIRE 720

Query: 728 WRSRVTFSSS 735
           WRSR T SSS
Sbjct: 721 WRSRATLSSS 729

BLAST of CSPI02G00340 vs. ExPASy TrEMBL
Match: A0A5A7UZZ3 (E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00420 PE=4 SV=1)

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 684/748 (91.44%), Postives = 704/748 (94.12%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           MTSVA+AVNGVRGGKGGGGSRRAVRWAVENLLPTADRF+LVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFVLVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQVFVPFKKLC+REKFL+LVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIK LVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQ---LRTLTEE- 247
           SRQWMLGDTDYY GSSA S KSLGT +S S LSI HQR DSIGVDSTEQ   L TLTEE 
Sbjct: 181 SRQWMLGDTDYYMGSSAVSAKSLGT-LSLSSLSISHQRGDSIGVDSTEQLNSLHTLTEEV 240

Query: 248 -----------------EDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYL 307
                            EDMQ+EVESLQLELETTVSLYKQACEELV TQKKVQSLT EYL
Sbjct: 241 FVFLFYCFIVSSFASISEDMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYL 300

Query: 308 EESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESV 367
           EESRKVT A+ERE++LRK AAKEKAKHLEAIKELEEAKDLLAKEAYERQLAEL+A+KESV
Sbjct: 301 EESRKVTAALEREESLRKAAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESV 360

Query: 368 EKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVF 427
           EK+KI+DTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYK SLDHTPVAIKV 
Sbjct: 361 EKEKIVDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVI 420

Query: 428 QHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKA 487
           QHDI+EKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHI LRNGKA
Sbjct: 421 QHDIYEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKA 480

Query: 488 PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD 547
           PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD
Sbjct: 481 PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD 540

Query: 548 IVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI 607
           IVPDNVTEY+NTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI
Sbjct: 541 IVPDNVTEYRNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI 600

Query: 608 ENSIASASLADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDF 667
           ENSIASASLADILDKSIS+WPLAKAEELARLALKCLKLRCRDRPDLESEVLP+LKRLVDF
Sbjct: 601 ENSIASASLADILDKSISDWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDF 660

Query: 668 ADTFQNEDNGFGNPPSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLK 727
           ADTFQ ED GFGNPPSHYFCPILQEVMEDPYIAADGF+YEYVAIKAWLEKHDVSP TKLK
Sbjct: 661 ADTFQKEDKGFGNPPSHYFCPILQEVMEDPYIAADGFTYEYVAIKAWLEKHDVSPVTKLK 720

Query: 728 LRHSFFIPNYTLRSAIREWRSRVTFSSS 735
           L+HSFFIPN+TLRSAIREWRSR T SSS
Sbjct: 721 LQHSFFIPNHTLRSAIREWRSRATLSSS 747

BLAST of CSPI02G00340 vs. ExPASy TrEMBL
Match: A0A5D3CHP5 (E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001070 PE=4 SV=1)

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 683/748 (91.31%), Postives = 704/748 (94.12%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           MTSVA+AVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQVFVPFKKLC+REKFL+LVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIK LVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQ---LRTLTEE- 247
           SRQWMLGDTDYY GSSA S KSLGT +S S LSI HQR DSIGVDSTEQ   L TLTEE 
Sbjct: 181 SRQWMLGDTDYYMGSSAVSAKSLGT-LSLSSLSISHQRGDSIGVDSTEQLNSLHTLTEEV 240

Query: 248 -----------------EDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYL 307
                            EDMQ+EVESLQLELETTVSLYKQACEELV TQKKVQSLT EYL
Sbjct: 241 FVFLFYCFIVSSFASISEDMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYL 300

Query: 308 EESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESV 367
           EESRKVT A+ERE++LRK AAKEKAKHLEAIKELEEAKDLLAKEAYERQLAEL+A+KESV
Sbjct: 301 EESRKVTAALEREESLRKAAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESV 360

Query: 368 EKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVF 427
           EK+KI+DTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYK SLDHTPVAIKV 
Sbjct: 361 EKEKIVDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVI 420

Query: 428 QHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKA 487
           QHDI+EKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHI LRNGKA
Sbjct: 421 QHDIYEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKA 480

Query: 488 PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD 547
           PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD
Sbjct: 481 PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD 540

Query: 548 IVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI 607
           IVPDNVTEY+NTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI
Sbjct: 541 IVPDNVTEYRNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI 600

Query: 608 ENSIASASLADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDF 667
           ENSIASASLADILDKSIS+WPLAKAEELARLALKCLKLRCRDRPDLESEVLP+LKRLVDF
Sbjct: 601 ENSIASASLADILDKSISDWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDF 660

Query: 668 ADTFQNEDNGFGNPPSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLK 727
           ADTFQ ED GFGNPPSHYFCPILQEVMEDPYIAADGF+YEYVAIKAWL+KHDVSP TKLK
Sbjct: 661 ADTFQKEDKGFGNPPSHYFCPILQEVMEDPYIAADGFTYEYVAIKAWLDKHDVSPVTKLK 720

Query: 728 LRHSFFIPNYTLRSAIREWRSRVTFSSS 735
           L+HSFFIPN+TLRSAI+EWRSR T SSS
Sbjct: 721 LQHSFFIPNHTLRSAIQEWRSRATSSSS 747

BLAST of CSPI02G00340 vs. ExPASy TrEMBL
Match: A0A6J1G4U5 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111450728 PE=4 SV=1)

HSP 1 Score: 1251.9 bits (3238), Expect = 0.0e+00
Identity = 644/730 (88.22%), Postives = 687/730 (94.11%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           MTSVA+A+NGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MTSVAVAINGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQ+FVPFKK+C+REKFL+LVETLILEDDNPATAL+RYA+ES
Sbjct: 61  SELDADVVALYVHDVKQKYEQIFVPFKKMCRREKFLLLVETLILEDDNPATALMRYATES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIK LVLGSCFRTCIAR+LKGDSVPSAIMR AS  FD+YV Y+RRVITR A+TAPS+ETD
Sbjct: 121 GIKHLVLGSCFRTCIARRLKGDSVPSAIMRAASRCFDVYVIYRRRVITRTATTAPSSETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLR---TLTEEE 247
           SRQWMLGDTDYY+GSS  SEKS GT +SSS +S VH+R DS+ V+STEQL    TLTEEE
Sbjct: 181 SRQWMLGDTDYYRGSSTVSEKSFGT-LSSSSMSNVHRRGDSLEVNSTEQLNSLSTLTEEE 240

Query: 248 DMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRK 307
           DMQ+EVESLQLELE+TVSLYKQACEELV  QKKVQSLT E LEESRKV  A+EREQALRK
Sbjct: 241 DMQAEVESLQLELESTVSLYKQACEELVHAQKKVQSLTSECLEESRKVNAALEREQALRK 300

Query: 308 VAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRR 367
           VAA+EKAKHLEAIKELEEAKD LAKEAYERQLAEL+ALKES+EK+KI+DTLLTNDRRYRR
Sbjct: 301 VAAEEKAKHLEAIKELEEAKDSLAKEAYERQLAELNALKESLEKKKIVDTLLTNDRRYRR 360

Query: 368 YTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEIL 427
           YTTAEIEAATNFF+EVNVIGEGGYGKVY  SLDHTPVAIKVF+HDIFE KDEFLKEVEIL
Sbjct: 361 YTTAEIEAATNFFDEVNVIGEGGYGKVYVCSLDHTPVAIKVFRHDIFENKDEFLKEVEIL 420

Query: 428 SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGL 487
           SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGK PLPWS RFRIVFQVASGL
Sbjct: 421 SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKPPLPWSIRFRIVFQVASGL 480

Query: 488 AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTL 547
           A LHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEY+NT+LAGTL
Sbjct: 481 ALLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTL 540

Query: 548 HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS 607
           HYMDPEYQRTGTLRP SDTYALGVTILQLLTGRQPHGLLLA+ENSIASASLADILDKSI+
Sbjct: 541 HYMDPEYQRTGTLRPNSDTYALGVTILQLLTGRQPHGLLLAVENSIASASLADILDKSIT 600

Query: 608 NWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHY 667
           +WP+A+AEELA LALKCLKLRCRDRPDLE+EVLP+LKRLVDFADTFQ    G  NPPSHY
Sbjct: 601 DWPVAEAEELAGLALKCLKLRCRDRPDLETEVLPVLKRLVDFADTFQ---KGAANPPSHY 660

Query: 668 FCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIRE 727
           FCPILQEVMEDPYIAADGF+YEY AIKAWLEK+DVSP TKLKL+HS FIPNYTLRSAI+E
Sbjct: 661 FCPILQEVMEDPYIAADGFTYEYEAIKAWLEKYDVSPVTKLKLQHSMFIPNYTLRSAIQE 720

Query: 728 WRSRVTFSSS 735
           WRSR+TFSSS
Sbjct: 721 WRSRLTFSSS 726

BLAST of CSPI02G00340 vs. NCBI nr
Match: XP_004141173.2 (U-box domain-containing protein 34 [Cucumis sativus] >KAE8651489.1 hypothetical protein Csa_019343 [Cucumis sativus])

HSP 1 Score: 1416.7 bits (3666), Expect = 0.0e+00
Identity = 732/734 (99.73%), Postives = 732/734 (99.73%), Query Frame = 0

Query: 1   MVVFSKAMTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP 60
           MVVFSKAMTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP
Sbjct: 1   MVVFSKAMTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP 60

Query: 61  MGDLVAVSELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATAL 120
           MGDLVAVSELD DVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATAL
Sbjct: 61  MGDLVAVSELDADVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATAL 120

Query: 121 LRYASESGIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKAST 180
           LRYASESGIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKAST
Sbjct: 121 LRYASESGIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKAST 180

Query: 181 APSTETDSRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLRTL 240
           APSTETDSRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLRTL
Sbjct: 181 APSTETDSRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQLRTL 240

Query: 241 TEEEDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQ 300
           TEEEDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQ
Sbjct: 241 TEEEDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQ 300

Query: 301 ALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDR 360
           ALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDR
Sbjct: 301 ALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDR 360

Query: 361 RYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKE 420
           RYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKE
Sbjct: 361 RYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKE 420

Query: 421 VEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQV 480
           VEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQV
Sbjct: 421 VEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQV 480

Query: 481 ASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVL 540
           ASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVT YQNTVL
Sbjct: 481 ASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTAYQNTVL 540

Query: 541 AGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILD 600
           AGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILD
Sbjct: 541 AGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILD 600

Query: 601 KSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNP 660
           KSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNP
Sbjct: 601 KSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNP 660

Query: 661 PSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRS 720
           PSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRS
Sbjct: 661 PSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRS 720

Query: 721 AIREWRSRVTFSSS 735
           AIREWRSRVTFSSS
Sbjct: 721 AIREWRSRVTFSSS 734

BLAST of CSPI02G00340 vs. NCBI nr
Match: XP_008459500.1 (PREDICTED: LOW QUALITY PROTEIN: U-box domain-containing protein 34-like [Cucumis melo])

HSP 1 Score: 1322.0 bits (3420), Expect = 0.0e+00
Identity = 682/730 (93.42%), Postives = 702/730 (96.16%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           MTSVA+AVNGVRGGKGGGGSRRAVRWAVENLLPTADRF+LVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFVLVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQVFVPFKKLC+REKFL+LVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIK LVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQ---LRTLTEEE 247
           SRQWMLGDTDYY GSSA S KSLGT +S S LSI HQR DSIGVDSTEQ   L TLTEEE
Sbjct: 181 SRQWMLGDTDYYMGSSAVSAKSLGT-LSLSSLSISHQRGDSIGVDSTEQLNSLHTLTEEE 240

Query: 248 DMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRK 307
           DMQ+EVESLQLELETTVSLYKQACEELV TQKKVQSLT EYLEESRKVT A+ERE++LRK
Sbjct: 241 DMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYLEESRKVTAALEREESLRK 300

Query: 308 VAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRR 367
            AAKEKAKHLEAIKELEEAKDLLAKEAYERQLAEL+A+KESVEK+KI+DTLLTNDRRYRR
Sbjct: 301 AAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESVEKEKIVDTLLTNDRRYRR 360

Query: 368 YTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEIL 427
           YTTAEIEAATNFFNEVNVIGEGGYGKVYK SLDHTPVAIKV QHDI+EKKDEFLKEVEIL
Sbjct: 361 YTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVIQHDIYEKKDEFLKEVEIL 420

Query: 428 SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGL 487
           SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHI LRNGKAPLPWSTRFRIVFQVASGL
Sbjct: 421 SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKAPLPWSTRFRIVFQVASGL 480

Query: 488 AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTL 547
           AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEY+NTVLAGTL
Sbjct: 481 AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTVLAGTL 540

Query: 548 HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS 607
           HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS
Sbjct: 541 HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS 600

Query: 608 NWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHY 667
           +WPLAKAEELARLALKCLKLRCRDRPDLESEVLP+LKRLVDFADTFQ ED GFGNPPSHY
Sbjct: 601 DWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDFADTFQKEDKGFGNPPSHY 660

Query: 668 FCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIRE 727
           FCPILQEVMEDPYIAADGF+YEYVAIKAWLEKHDVSP TKLKL+HSF  PN+TLRSAIRE
Sbjct: 661 FCPILQEVMEDPYIAADGFTYEYVAIKAWLEKHDVSPVTKLKLQHSFLXPNHTLRSAIRE 720

Query: 728 WRSRVTFSSS 735
           WRSR T SSS
Sbjct: 721 WRSRATLSSS 729

BLAST of CSPI02G00340 vs. NCBI nr
Match: KAA0061482.1 (U-box domain-containing protein 34-like [Cucumis melo var. makuwa])

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 684/748 (91.44%), Postives = 704/748 (94.12%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           MTSVA+AVNGVRGGKGGGGSRRAVRWAVENLLPTADRF+LVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFVLVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQVFVPFKKLC+REKFL+LVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIK LVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQ---LRTLTEE- 247
           SRQWMLGDTDYY GSSA S KSLGT +S S LSI HQR DSIGVDSTEQ   L TLTEE 
Sbjct: 181 SRQWMLGDTDYYMGSSAVSAKSLGT-LSLSSLSISHQRGDSIGVDSTEQLNSLHTLTEEV 240

Query: 248 -----------------EDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYL 307
                            EDMQ+EVESLQLELETTVSLYKQACEELV TQKKVQSLT EYL
Sbjct: 241 FVFLFYCFIVSSFASISEDMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYL 300

Query: 308 EESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESV 367
           EESRKVT A+ERE++LRK AAKEKAKHLEAIKELEEAKDLLAKEAYERQLAEL+A+KESV
Sbjct: 301 EESRKVTAALEREESLRKAAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESV 360

Query: 368 EKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVF 427
           EK+KI+DTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYK SLDHTPVAIKV 
Sbjct: 361 EKEKIVDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVI 420

Query: 428 QHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKA 487
           QHDI+EKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHI LRNGKA
Sbjct: 421 QHDIYEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKA 480

Query: 488 PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD 547
           PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD
Sbjct: 481 PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD 540

Query: 548 IVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI 607
           IVPDNVTEY+NTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI
Sbjct: 541 IVPDNVTEYRNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI 600

Query: 608 ENSIASASLADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDF 667
           ENSIASASLADILDKSIS+WPLAKAEELARLALKCLKLRCRDRPDLESEVLP+LKRLVDF
Sbjct: 601 ENSIASASLADILDKSISDWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDF 660

Query: 668 ADTFQNEDNGFGNPPSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLK 727
           ADTFQ ED GFGNPPSHYFCPILQEVMEDPYIAADGF+YEYVAIKAWLEKHDVSP TKLK
Sbjct: 661 ADTFQKEDKGFGNPPSHYFCPILQEVMEDPYIAADGFTYEYVAIKAWLEKHDVSPVTKLK 720

Query: 728 LRHSFFIPNYTLRSAIREWRSRVTFSSS 735
           L+HSFFIPN+TLRSAIREWRSR T SSS
Sbjct: 721 LQHSFFIPNHTLRSAIREWRSRATLSSS 747

BLAST of CSPI02G00340 vs. NCBI nr
Match: TYK10792.1 (U-box domain-containing protein 34-like [Cucumis melo var. makuwa])

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 683/748 (91.31%), Postives = 704/748 (94.12%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           MTSVA+AVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MTSVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQVFVPFKKLC+REKFL+LVETLILEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLILEDDNPATALLRYASES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIK LVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD
Sbjct: 121 GIKCLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQ---LRTLTEE- 247
           SRQWMLGDTDYY GSSA S KSLGT +S S LSI HQR DSIGVDSTEQ   L TLTEE 
Sbjct: 181 SRQWMLGDTDYYMGSSAVSAKSLGT-LSLSSLSISHQRGDSIGVDSTEQLNSLHTLTEEV 240

Query: 248 -----------------EDMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYL 307
                            EDMQ+EVESLQLELETTVSLYKQACEELV TQKKVQSLT EYL
Sbjct: 241 FVFLFYCFIVSSFASISEDMQAEVESLQLELETTVSLYKQACEELVHTQKKVQSLTSEYL 300

Query: 308 EESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESV 367
           EESRKVT A+ERE++LRK AAKEKAKHLEAIKELEEAKDLLAKEAYERQLAEL+A+KESV
Sbjct: 301 EESRKVTAALEREESLRKAAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELNAIKESV 360

Query: 368 EKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVF 427
           EK+KI+DTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYK SLDHTPVAIKV 
Sbjct: 361 EKEKIVDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKCSLDHTPVAIKVI 420

Query: 428 QHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKA 487
           QHDI+EKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHI LRNGKA
Sbjct: 421 QHDIYEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHIFLRNGKA 480

Query: 488 PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD 547
           PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD
Sbjct: 481 PLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGD 540

Query: 548 IVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI 607
           IVPDNVTEY+NTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI
Sbjct: 541 IVPDNVTEYRNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAI 600

Query: 608 ENSIASASLADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDF 667
           ENSIASASLADILDKSIS+WPLAKAEELARLALKCLKLRCRDRPDLESEVLP+LKRLVDF
Sbjct: 601 ENSIASASLADILDKSISDWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDF 660

Query: 668 ADTFQNEDNGFGNPPSHYFCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLK 727
           ADTFQ ED GFGNPPSHYFCPILQEVMEDPYIAADGF+YEYVAIKAWL+KHDVSP TKLK
Sbjct: 661 ADTFQKEDKGFGNPPSHYFCPILQEVMEDPYIAADGFTYEYVAIKAWLDKHDVSPVTKLK 720

Query: 728 LRHSFFIPNYTLRSAIREWRSRVTFSSS 735
           L+HSFFIPN+TLRSAI+EWRSR T SSS
Sbjct: 721 LQHSFFIPNHTLRSAIQEWRSRATSSSS 747

BLAST of CSPI02G00340 vs. NCBI nr
Match: XP_038891123.1 (U-box domain-containing protein 34-like [Benincasa hispida])

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 664/730 (90.96%), Postives = 694/730 (95.07%), Query Frame = 0

Query: 8   MTSVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 67
           M SVA+AVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV
Sbjct: 1   MISVAVAVNGVRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 60

Query: 68  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 127
           SELD DVVALYVHDVKQKYEQVFVPFKKLC+REKFL+LVETL+LEDDNPATALLRYASES
Sbjct: 61  SELDADVVALYVHDVKQKYEQVFVPFKKLCRREKFLLLVETLVLEDDNPATALLRYASES 120

Query: 128 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 187
           GIK LVLGSCFRTC+ARKLKGDSVPSAIMRT  SSFDI+V YKRRVITRKASTAPS ETD
Sbjct: 121 GIKRLVLGSCFRTCVARKLKGDSVPSAIMRTVHSSFDIHVIYKRRVITRKASTAPSNETD 180

Query: 188 SRQWMLGDTDYYKGSSADSEKSLGTDMSSSYLSIVHQRDDSIGVDSTEQ---LRTLTEEE 247
           SRQWMLGDTDYY+GSSA SEKSLGT  SSS LSI H+R DS+ V+STE+   L TLTEEE
Sbjct: 181 SRQWMLGDTDYYRGSSAASEKSLGT-FSSSSLSIGHRRGDSLKVNSTEELNSLNTLTEEE 240

Query: 248 DMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRK 307
           DMQ+EVESLQLELETTVSLYKQACEELV  QKKVQSLT E LEESR+V  A+EREQALRK
Sbjct: 241 DMQAEVESLQLELETTVSLYKQACEELVHAQKKVQSLTSECLEESRRVNAALEREQALRK 300

Query: 308 VAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRR 367
             AKEK KHLEAIKELEEAKDLLAKEAYERQLAEL+ALKESVEKQKI+DTLLTNDRRYRR
Sbjct: 301 TVAKEKEKHLEAIKELEEAKDLLAKEAYERQLAELNALKESVEKQKIVDTLLTNDRRYRR 360

Query: 368 YTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEIL 427
           YTTAEIEAATNFF+EVNVIGEGGYGKVY  SLDHTPVAIKVFQHDIF+KKDEFLKEVEIL
Sbjct: 361 YTTAEIEAATNFFDEVNVIGEGGYGKVYNCSLDHTPVAIKVFQHDIFKKKDEFLKEVEIL 420

Query: 428 SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGL 487
           SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGL
Sbjct: 421 SQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGL 480

Query: 488 AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTL 547
           AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEY+NT+LAGTL
Sbjct: 481 AFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYRNTILAGTL 540

Query: 548 HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS 607
           HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS
Sbjct: 541 HYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSIS 600

Query: 608 NWPLAKAEELARLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHY 667
           +WPLAKAEELARLALKCLKLRCRDRPDLESEVLP+LKRLVDFADT +NED GF NP SHY
Sbjct: 601 DWPLAKAEELARLALKCLKLRCRDRPDLESEVLPVLKRLVDFADTCENEDKGFANPLSHY 660

Query: 668 FCPILQEVMEDPYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIRE 727
           FCPILQE+MEDPYIAADGF+YEYVAIKAWLEK+D+SP TKLKL+HS FIPN+TLRSAI+E
Sbjct: 661 FCPILQEIMEDPYIAADGFTYEYVAIKAWLEKYDISPVTKLKLQHSLFIPNHTLRSAIQE 720

Query: 728 WRSRVTFSSS 735
           WRSRVT SSS
Sbjct: 721 WRSRVTVSSS 729

BLAST of CSPI02G00340 vs. TAIR 10
Match: AT5G57035.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 768.5 bits (1983), Expect = 5.0e-222
Identity = 405/766 (52.87%), Postives = 539/766 (70.37%), Query Frame = 0

Query: 9   TSVAIAVNG-VRGGKGGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTPMGDLVAV 68
           +SV++AV G V    GG  SRRA+RW +EN LP  DR +LVHVMP +T+IP+P G  + +
Sbjct: 19  SSVSVAVKGSVGDAVGGTASRRALRWTIENFLPKIDRLVLVHVMPTVTTIPSPSGSKIPI 78

Query: 69  SELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASES 128
            ELDE VV++Y  D+++++EQVFVPFK++CK  K    VETL+LE  +PA ALL+Y S++
Sbjct: 79  EELDESVVSMYKRDLRKEFEQVFVPFKRICKSNK----VETLLLEHHDPAKALLKYMSDT 138

Query: 129 GIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVITRKASTAPSTETD 188
            ++ LV+GSC    + RK KG  +P  ++  A  + +IYV  K R++T+  +   +  + 
Sbjct: 139 DVECLVIGSCSSNFLTRK-KGQEMPLTVLGEAPETCEIYVVCKDRILTKSTNQFTADSSS 198

Query: 189 SRQWMLGDTDYYK----------GSSADSEKSLG--------------------TDMSSS 248
           S +   G   Y +          G SA S  S G                    +D  SS
Sbjct: 199 SFRIPEGAEAYTESFSRTRSDKTGLSASSITSSGRMRIGRPGSLPHSHPTSRVYSDAQSS 258

Query: 249 YLSIVHQRDD--------------SIGVDSTEQLRTLTEEEDMQSEVESLQLELETTVSL 308
              IV   D+               I +D    L+  T +  +++EVE L+ E++TT+S+
Sbjct: 259 STDIVLVDDEHCRSILRHSTVSTSKIQMDPRPHLK--TPKSGVRAEVEQLRKEVQTTLSM 318

Query: 309 YKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEA 368
           YKQACEELV  Q +VQSL+ E ++E+ +V  A+E+E+  RK AA+EK KHL+A+KE+EEA
Sbjct: 319 YKQACEELVHKQTQVQSLSSECIKETERVITALEKEEMRRKAAAEEKEKHLKAVKEVEEA 378

Query: 369 KDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVNVI 428
           K +LAKE  ERQLAELDALK+S+EKQK+I+ L   D RYR+YT  EI AAT+ F+   +I
Sbjct: 379 KSMLAKEFCERQLAELDALKQSIEKQKVIEQLFLRDGRYRKYTKEEIAAATDNFSSRKII 438

Query: 429 GEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERG 488
           GEGGYGKVYK SLDHTPVA+KV + D  EKK+EFLKE+ +LSQ+RHPHVVLLLGACPE G
Sbjct: 439 GEGGYGKVYKCSLDHTPVALKVLKPDSVEKKEEFLKEISVLSQLRHPHVVLLLGACPENG 498

Query: 489 CLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPG 548
           CL+YEYMENGSLD HI  + GK  L W  RFRI+++ A GLAFLHNSKPEPI+HRDLKPG
Sbjct: 499 CLVYEYMENGSLDCHISPKKGKPSLSWFIRFRIIYETACGLAFLHNSKPEPIVHRDLKPG 558

Query: 549 NILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDT 608
           NILLDRNFVSKI DVG+AK++ D  PD+VT Y+N+++AGTL+YMDPEYQRTGT+RPKSD 
Sbjct: 559 NILLDRNFVSKIGDVGLAKLMSDEAPDSVTVYRNSIIAGTLYYMDPEYQRTGTIRPKSDL 618

Query: 609 YALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISNWPLAKAEELARLALKCLK 668
           YA G+ ILQLLT R P+GLL  +E+++      D+LD S+ +WP+A+A+ELAR+A++C +
Sbjct: 619 YAFGIIILQLLTARHPNGLLFCVEDAVKRGCFEDMLDGSVKDWPIAEAKELARIAIRCSQ 678

Query: 669 LRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHYFCPILQEVMEDPYIAADGF 728
           L+CRDRPDL ++VLP LKR+++ A++    +      P+HY+CPIL+E+MEDP IAADGF
Sbjct: 679 LKCRDRPDLSTQVLPALKRILESANSRLKTEQANARAPTHYYCPILKEIMEDPQIAADGF 738

BLAST of CSPI02G00340 vs. TAIR 10
Match: AT2G19410.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 705.7 bits (1820), Expect = 4.0e-203
Identity = 392/778 (50.39%), Postives = 523/778 (67.22%), Query Frame = 0

Query: 11  VAIAVNGVRGGK-GGGGSRRAVRWAVENLLPTADRFILVHVMPKITSIPTP--------- 70
           VA+AV G+ G K GG GSRRAVRWAV+NLLP AD+F+++HV+P ITSIPTP         
Sbjct: 24  VAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHVIPTITSIPTPNILILMFTR 83

Query: 71  -----MGDLVAVSELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFL-ILVETLILEDD 130
                 GD + V E++E VV +YV DVK++YE VFVPF K+CK  +       +   +  
Sbjct: 84  MWVVTAGDRLPVEEVEESVVEMYVRDVKKEYETVFVPFLKMCKSTRSTKRYFRSRRTKGT 143

Query: 131 NPATALLRYASESGIKSLVLGSCFRTCIARKLKGDSVPSAIMRTASSSFDIYVKYKRRVI 190
                +LRYA E+    +V      T     L  +  P      A+++ D    +     
Sbjct: 144 GVPLTVLRYAPETCEVYIVCKDRITTKSMDPLI-NREPCTSPHAAATAHDFLRDWAASFH 203

Query: 191 TRKASTAP----STETDSRQWMLG----------------DTDYYKGSSADS----EKSL 250
           T ++ T P    STE  +R+                         K SSA +     +  
Sbjct: 204 TLRSPTLPDPRQSTEAGTRRSASARELRFEALSLTCNKPKTPQSSKASSATTPEIFRRRR 263

Query: 251 GTDM---------------SSSYLSIVHQRDDSIGVDSTEQLRTLTEEEDMQSEVESLQL 310
           G+D+                S+  +IV +  DS    S  +    +++ +++ EVE L+ 
Sbjct: 264 GSDIPQLNYSDFDKTCTKPQSNVENIVSEHRDS--DRSPPETSRKSKKVEIEEEVERLKN 323

Query: 311 ELETTVSLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAAKEKAKHLE 370
           EL++TV  YKQACEEL  TQ KV+ L+ EYL ES++V +AVE+E+  R  AA EK ++++
Sbjct: 324 ELQSTVFKYKQACEELFSTQNKVKMLSTEYLNESKRVNNAVEKEELQRNTAALEKERYMK 383

Query: 371 AIKELEEAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATN 430
           A+KE+E AK LLA+E  +RQ+AE++AL+  +EK+K+ID LL  D RYR+YT  EI  AT 
Sbjct: 384 AVKEVETAKALLAREFCQRQIAEVNALRTYLEKKKVIDQLLGTDHRYRKYTIEEIVTATE 443

Query: 431 FFNEVNVIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLL 490
            F+   VIGEGGYGKVY+ SLD TP A+KV + D  EKK EFLKEVE+LSQ+RHPHVVLL
Sbjct: 444 GFSPEKVIGEGGYGKVYQCSLDSTPAAVKVVRLDTPEKKQEFLKEVEVLSQLRHPHVVLL 503

Query: 491 LGACPERGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPI 550
           LGACPE GCL+YEY+ENGSL+++I  R  K PLPW  RFR++F+VA GLAFLH+SKPEPI
Sbjct: 504 LGACPENGCLVYEYLENGSLEEYIFHRKNKPPLPWFIRFRVIFEVACGLAFLHSSKPEPI 563

Query: 551 IHRDLKPGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTLHYMDPEYQRTG 610
           +HRDLKPGNILL+RN+VSKI+DVG+AK++ D+ PDNVT Y+N+VLAGTLHY+DPEY RTG
Sbjct: 564 VHRDLKPGNILLNRNYVSKIADVGLAKLVTDVAPDNVTMYRNSVLAGTLHYIDPEYHRTG 623

Query: 611 TLRPKSDTYALGVTILQLLTGRQPHGLLLAIENSIASASLADILDKSISNWPLAKAEELA 670
           T+RPKSD YA G+ ILQLLT R P G++ A+EN++   +L ++LDKS+++WPLA+ EELA
Sbjct: 624 TIRPKSDLYAFGIIILQLLTARNPSGIVPAVENAVKKGTLTEMLDKSVTDWPLAETEELA 683

Query: 671 RLALKCLKLRCRDRPDLESEVLPILKRLVDFADTFQNEDNGFGNPPSHYFCPILQEVMED 730
           R+ LKC + RCRDRPDL+SEV+P+LKRLV+ A++   ++      PSHYFCPIL+E+ME+
Sbjct: 684 RIGLKCAEFRCRDRPDLKSEVIPVLKRLVETANSKVKKEGSNLRAPSHYFCPILREIMEE 743

Query: 731 PYIAADGFSYEYVAIKAWLEKHDVSPATKLKLRHSFFIPNYTLRSAIREWRSRVTFSS 734
           P IAADGF+YE  AI AWLEKH++SP T+ KL H    PN+TLRSAIR+W+SRV FS+
Sbjct: 744 PEIAADGFTYERKAILAWLEKHNISPVTRQKLDHFKLTPNHTLRSAIRDWKSRVRFSN 798

BLAST of CSPI02G00340 vs. TAIR 10
Match: AT5G61550.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 4.2e-136
Identity = 312/828 (37.68%), Postives = 450/828 (54.35%), Query Frame = 0

Query: 10  SVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDLVAVS 69
           SVA+A+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 70  ELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASESG 129
           EL EDVV+ Y  ++     ++  P+KK+ +R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 130 IKSLVLGSCFRTCIARKLKGDS-----VPS------------AIMRTASSSFDIYVKYKR 189
           +  LV+G   R   +RK+   S     VP             A +R + S     ++++R
Sbjct: 143 VTKLVIGMSLRGFFSRKIDMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIRFER 202

Query: 190 RVITRKASTAPSTETDSRQWMLGDTD-------------YYKGSSADSE---KSLGTDM- 249
              T  ++ +P    + + ++   ++             +  GS+A ++    S GTD  
Sbjct: 203 SSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGTDQE 262

Query: 250 ---SSSYLSIVH-------QRDDS------IGVDS------TEQLRTLTEEEDMQS---- 309
              +   + IVH        +D+S      +G ++      T + R   +  +M+S    
Sbjct: 263 EVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSSSSS 322

Query: 310 -----------------------------------------------EVESLQLELETTV 369
                                                          E+E L+ EL+   
Sbjct: 323 NNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELKHVQ 382

Query: 370 SLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAAKEKAKHLEAIKELE 429
            +Y  A  E V   KK+  L Q   EES K+ +  E+E+  +  A+KEK ++ EA+KE E
Sbjct: 383 EMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMKEAE 442

Query: 430 EAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVN 489
           + K+L+ KEA  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI AAT+ F E  
Sbjct: 443 KVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFAENL 502

Query: 490 VIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPE 549
            IG G YG VYK +L HT  A+KV      +   +F +E+EILS+IRHPH+VLLLGACPE
Sbjct: 503 KIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGACPE 562

Query: 550 RGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLK 609
           RGCL+YEYM+NGSLDD ++L N   P+PW  RFRI  +VAS L FLH SKP PIIHRDLK
Sbjct: 563 RGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHRDLK 622

Query: 610 PGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKS 669
           PGNILLD NFVSK+ DVG++ ++      + T ++ T   GTL Y+DPEYQRTG + PKS
Sbjct: 623 PGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTGIISPKS 682

Query: 670 DTYALGVTILQLLTGRQPHGLLLAIENSIA-SASLADILDKSISNWPLAKAEELARLALK 728
           D Y+LGV ILQL+T +    +   +E +I   A    ILDK   +WP++   ELA L L 
Sbjct: 683 DVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTRELAALGLC 742

BLAST of CSPI02G00340 vs. TAIR 10
Match: AT5G61550.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 4.2e-136
Identity = 312/828 (37.68%), Postives = 450/828 (54.35%), Query Frame = 0

Query: 10  SVAIAVNGVRGGKGGGGSRRAVRWAVENLLPTA-DRFILVHVMPKITSIPTPMGDLVAVS 69
           SVA+A+NG +       S+  V WA+E  +P     F L++V P ++ IPTPMG  VAVS
Sbjct: 23  SVAVAINGKK------KSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVS 82

Query: 70  ELDEDVVALYVHDVKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASESG 129
           EL EDVV+ Y  ++     ++  P+KK+ +R K  + VE L+L+   PA A+    + +G
Sbjct: 83  ELREDVVSAYKQELDWSANEMLRPYKKMFERRK--VQVEVLLLDSLEPAAAIAEEIAGTG 142

Query: 130 IKSLVLGSCFRTCIARKLKGDS-----VPS------------AIMRTASSSFDIYVKYKR 189
           +  LV+G   R   +RK+   S     VP             A +R + S     ++++R
Sbjct: 143 VTKLVIGMSLRGFFSRKIDMSSLIATAVPRFCTVYVISKGKLASVRPSESDASGSIRFER 202

Query: 190 RVITRKASTAPSTETDSRQWMLGDTD-------------YYKGSSADSE---KSLGTDM- 249
              T  ++ +P    + + ++   ++             +  GS+A ++    S GTD  
Sbjct: 203 SSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGTDQE 262

Query: 250 ---SSSYLSIVH-------QRDDS------IGVDS------TEQLRTLTEEEDMQS---- 309
              +   + IVH        +D+S      +G ++      T + R   +  +M+S    
Sbjct: 263 EVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRREMRSSSSS 322

Query: 310 -----------------------------------------------EVESLQLELETTV 369
                                                          E+E L+ EL+   
Sbjct: 323 NNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKLRAELKHVQ 382

Query: 370 SLYKQACEELVRTQKKVQSLTQEYLEESRKVTDAVEREQALRKVAAKEKAKHLEAIKELE 429
            +Y  A  E V   KK+  L Q   EES K+ +  E+E+  +  A+KEK ++ EA+KE E
Sbjct: 383 EMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRYEEAMKEAE 442

Query: 430 EAKDLLAKEAYERQLAELDALKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVN 489
           + K+L+ KEA  R+ AE  A +++ EK K+  +L++   +Y+ YT  EI AAT+ F E  
Sbjct: 443 KVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAATSDFAENL 502

Query: 490 VIGEGGYGKVYKSSLDHTPVAIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPE 549
            IG G YG VYK +L HT  A+KV      +   +F +E+EILS+IRHPH+VLLLGACPE
Sbjct: 503 KIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLVLLLGACPE 562

Query: 550 RGCLIYEYMENGSLDDHILLRNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLK 609
           RGCL+YEYM+NGSLDD ++L N   P+PW  RFRI  +VAS L FLH SKP PIIHRDLK
Sbjct: 563 RGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHKSKPRPIIHRDLK 622

Query: 610 PGNILLDRNFVSKISDVGMAKIIGDIVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKS 669
           PGNILLD NFVSK+ DVG++ ++      + T ++ T   GTL Y+DPEYQRTG + PKS
Sbjct: 623 PGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQRTGIISPKS 682

Query: 670 DTYALGVTILQLLTGRQPHGLLLAIENSIA-SASLADILDKSISNWPLAKAEELARLALK 728
           D Y+LGV ILQL+T +    +   +E +I   A    ILDK   +WP++   ELA L L 
Sbjct: 683 DVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDTRELAALGLC 742

BLAST of CSPI02G00340 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 447.6 bits (1150), Expect = 1.9e-125
Identity = 293/809 (36.22%), Postives = 430/809 (53.15%), Query Frame = 0

Query: 23  GGGGSRRAVRWAVENLLPTAD-RFILVHVMPKITSIPTPMGDLVAVSELDEDVVALYVHD 82
           G   S+  V WA+E      +  F L+H+ P ITS+PTPMG+ + +SE+ +DVV  Y  +
Sbjct: 28  GSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVTAYRQE 87

Query: 83  VKQKYEQVFVPFKKLCKREKFLILVETLILEDDNPATALLRYASESGIKSLVLGSCFRTC 142
           +  + E++  P+ KL  R K  + VE L++E DN A A+    +   I  +V+G   R+ 
Sbjct: 88  ILWQSEEMLKPYTKLFVRRK--VAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGSSRSF 147

Query: 143 IARKLKGDSVPSAIM----------------------------------RTASSS----- 202
            +RK    SV SA+M                                  RT SSS     
Sbjct: 148 FSRKADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSSSGSSGP 207

Query: 203 ------------------FDIYVKYKRR--VITRKAS----------------------- 262
                               + V+  +    I  +AS                       
Sbjct: 208 TSDSSDVMSSAHDSQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMSLDAEEA 267

Query: 263 ------TAPSTETDSRQWMLGDTDYYKGSSADSEKS-------LGTDMSSSYLSI--VHQ 322
                    ST+T SR W     DY +   A S  S        GT  S S + +   H 
Sbjct: 268 RDVSSINRSSTDTTSR-WTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGVDTTHS 327

Query: 323 RDDSIGVDSTEQL--RTLTEEE-DMQSEVESLQLELETTVSLYKQACEELVRTQKKVQSL 382
           R      + ++ L  ++ T+ + ++  EVE L+ EL     +Y  A  E     +K+  L
Sbjct: 328 RASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASRKLGEL 387

Query: 383 TQEYLEESRKVTDAVEREQALRKVAAKEKAKHLEAIKELEEAKDLLAKEAYERQLAELDA 442
            Q  LEE+ K+ +   +E   R++A KEK    +A ++ E  ++   +E  +R+ AE  +
Sbjct: 388 NQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQRREAERKS 447

Query: 443 LKESVEKQKIIDTLLTNDRRYRRYTTAEIEAATNFFNEVNVIGEGGYGKVYKSSLDHTPV 502
            +++ EK+K+  TL +   +Y+ +   EI AAT+ F+E   IG G YG VYK +L HT  
Sbjct: 448 ARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYKCNLHHTTA 507

Query: 503 AIKVFQHDIFEKKDEFLKEVEILSQIRHPHVVLLLGACPERGCLIYEYMENGSLDDHILL 562
            +KV Q    +   +F +E+EILS+IRHPH+VLLLGACPE+G L+YEYMENGSL+D +  
Sbjct: 508 VVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQ 567

Query: 563 RNGKAPLPWSTRFRIVFQVASGLAFLHNSKPEPIIHRDLKPGNILLDRNFVSKISDVGMA 622
            N   PLPW  RFRI ++VA+ L FLH SKP+PIIHRDLKP NILLD NFVSK+ DVG++
Sbjct: 568 VNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLS 627

Query: 623 KIIG-DIVPDNVTEYQNTVLAGTLHYMDPEYQRTGTLRPKSDTYALGVTILQLLTGRQPH 682
            ++  D +    T Y+ T   GTL Y+DPEYQRTG +  KSD Y+ G+ +LQLLT +   
Sbjct: 628 TMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKPAI 687

Query: 683 GLLLAIENSI-ASASLADILDKSISNWPLAKAEELARLALKCLKLRCRDRPDLESEVLPI 728
            L   +E+++ ++     ILD+   NWP+ +  ELA LAL C +LR +DRPDL+ ++LP 
Sbjct: 688 ALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPA 747

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8S8S75.6e-20250.39U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3... [more]
Q9FKG65.9e-13537.68U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2... [more]
Q9SW112.7e-12436.22U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2... [more]
Q9LU475.0e-11835.06Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q9FKG51.5e-10640.00U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0LKM60.0e+0099.72E3 ubiquitin ligase OS=Cucumis sativus OX=3659 GN=Csa_2G000350 PE=4 SV=1[more]
A0A1S3C9U00.0e+0093.42E3 ubiquitin ligase OS=Cucumis melo OX=3656 GN=LOC103498614 PE=4 SV=1[more]
A0A5A7UZZ30.0e+0091.44E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G0... [more]
A0A5D3CHP50.0e+0091.31E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G... [more]
A0A6J1G4U50.0e+0088.22E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111450728 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004141173.20.0e+0099.73U-box domain-containing protein 34 [Cucumis sativus] >KAE8651489.1 hypothetical ... [more]
XP_008459500.10.0e+0093.42PREDICTED: LOW QUALITY PROTEIN: U-box domain-containing protein 34-like [Cucumis... [more]
KAA0061482.10.0e+0091.44U-box domain-containing protein 34-like [Cucumis melo var. makuwa][more]
TYK10792.10.0e+0091.31U-box domain-containing protein 34-like [Cucumis melo var. makuwa][more]
XP_038891123.10.0e+0090.96U-box domain-containing protein 34-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G57035.15.0e-22252.87U-box domain-containing protein kinase family protein [more]
AT2G19410.14.0e-20350.39U-box domain-containing protein kinase family protein [more]
AT5G61550.14.2e-13637.68U-box domain-containing protein kinase family protein [more]
AT5G61550.24.2e-13637.68U-box domain-containing protein kinase family protein [more]
AT4G25160.11.9e-12536.22U-box domain-containing protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 264..291
NoneNo IPR availableCOILSCoilCoilcoord: 303..349
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 450..656
e-value: 1.3E-47
score: 163.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 356..449
e-value: 2.8E-24
score: 87.0
NoneNo IPR availablePIRSRPIRSR000605-50PIRSR000605-50coord: 336..580
e-value: 1.7E-17
score: 61.2
NoneNo IPR availablePANTHERPTHR45647:SF65U-BOX DOMAIN-CONTAINING PROTEIN 34coord: 230..728
coord: 9..217
NoneNo IPR availablePANTHERPTHR45647OS02G0152300 PROTEINcoord: 9..217
NoneNo IPR availablePANTHERPTHR45647OS02G0152300 PROTEINcoord: 230..728
NoneNo IPR availableCDDcd16655RING-Ubox_WDSUB1_likecoord: 664..704
e-value: 2.95761E-16
score: 71.0959
NoneNo IPR availableCDDcd01989STK_Ncoord: 30..167
e-value: 1.71175E-37
score: 135.096
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 650..728
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 25..157
IPR003613U box domainSMARTSM00504Ubox_2coord: 663..725
e-value: 2.5E-21
score: 86.9
IPR003613U box domainPFAMPF04564U-boxcoord: 661..726
e-value: 3.7E-13
score: 49.4
IPR003613U box domainPROSITEPS51698U_BOXcoord: 659..732
score: 27.432281
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 377..640
e-value: 4.3E-36
score: 135.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 378..633
e-value: 2.5E-41
score: 141.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 377..640
score: 36.68269
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 659..731
e-value: 1.7E-24
score: 87.5
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 10..163
e-value: 1.6E-10
score: 43.5
IPR006016UspAPFAMPF00582Uspcoord: 26..160
e-value: 2.1E-7
score: 31.5
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 495..507
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 383..404
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 360..633

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G00340.1CSPI02G00340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0004672 protein kinase activity