CSPI01G34880 (gene) Cucumber (PI 183967) v1

BLAST of CSPI01G34880 vs. ExPASy Swiss-Prot
Match: O04893 (Alpha-glucosidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 524/872 (60.09%), Postives = 656/872 (75.23%), Query Frame = 0

Query: 53  VGLGYRIRSSHVDP-AGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDST 112
           +G GY+++S  VD    ++LTA   L+  S VYGPD+  L++ A+ ES DRLRVRITD+ 
Sbjct: 36  IGYGYKVKSVKVDSGTRRSLTALPQLVKNSSVYGPDIQLLSITASLESNDRLRVRITDAK 95

Query: 113 RERWEIPHHIL---------PRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAP 172
             RWEIP +IL         P   SSL R+L  +   + +   +SHP SDL F+L +T P
Sbjct: 96  HRRWEIPDNILHRHQPPPPPPHSLSSLYRTLLSSPTTNRRKILLSHPNSDLTFSLINTTP 155

Query: 173 FGFSVLRRSSGDVLFDTSPAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFK 232
           FGF++ R+S+ DVLFD +P  ++  TFL+F DQY+ L+SSLP  R+ I+G+GE ++ +F+
Sbjct: 156 FGFTISRKSTHDVLFDATPDPTNPNTFLIFIDQYLHLTSSLPGTRAHIYGLGEHSKPTFQ 215

Query: 233 LVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGM 292
           L    N+TLT+  ADI S N DVNLYG+HPFY+D+RS        AG+THGVLLLNSNGM
Sbjct: 216 LA--HNQTLTMRAADIPSSNPDVNLYGSHPFYMDVRS-----SPVAGSTHGVLLLNSNGM 275

Query: 293 DIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYK 352
           D+ Y+G+RITYKVIGGIIDLYFFAGPSP  V++Q+T +IGRPAP+PYW+FGF QCRYGY 
Sbjct: 276 DVEYTGNRITYKVIGGIIDLYFFAGPSPGQVVEQFTRVIGRPAPMPYWAFGFQQCRYGYH 335

Query: 353 NVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQK 412
           +V +++SVVA YAKA IPLE MWTDIDYMD YKDFT DP+NFP  KMK FV+NLHKNGQK
Sbjct: 336 DVYELQSVVAGYAKAKIPLEVMWTDIDYMDAYKDFTLDPVNFPLDKMKKFVNNLHKNGQK 395

Query: 413 YVLILDPGISTNNTYGPYIRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGR 472
           YV+ILDPGISTN TY  YIRG K D+F+K NG PYLG VWPGPVYFPDF  P + TFW  
Sbjct: 396 YVVILDPGISTNKTYETYIRGMKHDVFLKRNGKPYLGSVWPGPVYFPDFLKPSALTFWTD 455

Query: 473 EIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASI 532
           EI+ F +++P DGLWIDMNEISNFI+S   P S LDNPPY INN+ V  P+ NKT+P + 
Sbjct: 456 EIKRFLNLLPVDGLWIDMNEISNFISSPPIPGSTLDNPPYKINNSGVMLPIINKTIPPTA 515

Query: 533 LHFGNLTEYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGAT 592
           +H+G++ EYN HNL+G+LE+R T A+L+K+T +RPFVLSRSTF GSGKYTAHWTGDN AT
Sbjct: 516 MHYGDIPEYNVHNLFGYLEARVTRAALIKLTEKRPFVLSRSTFSGSGKYTAHWTGDNAAT 575

Query: 593 WNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSI 652
           WNDL Y+IPS+L+FGLFGIPMVG+DICGF G+TTEELCRRWIQLGAFYPF+RDHS  G+ 
Sbjct: 576 WNDLVYSIPSMLDFGLFGIPMVGADICGFLGNTTEELCRRWIQLGAFYPFSRDHSSLGTT 635

Query: 653 RQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEID 712
            QELY W+SVAASARKVL LRY LLPYFYTLMYEA   G PIARPLFFSFP DIKT+ I 
Sbjct: 636 YQELYRWESVAASARKVLGLRYTLLPYFYTLMYEAQLNGIPIARPLFFSFPDDIKTYGIS 695

Query: 713 SQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINV 772
           SQFLLG GV+VSPVLK G  SV AYFP GNWF LF+Y+  V  ++G+ + L AP DHINV
Sbjct: 696 SQFLLGKGVMVSPVLKPGVVSVTAYFPRGNWFDLFDYTRSVTASTGRYVTLSAPPDHINV 755

Query: 773 HVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSM 832
           H++EGNILA+ G+AMTT+AA++TP+ LLVV+S+  +SFGE+FLDDG  V MG   G W+ 
Sbjct: 756 HIQEGNILAMQGKAMTTQAARKTPFHLLVVMSDCGASFGELFLDDGVEVTMGVNRGKWTF 815

Query: 833 VRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNG 892
           V+F + +     ++ S V++G FA+SQK +IDKVT +G ++  K+    +          
Sbjct: 816 VKFIAASAKQTCIITSDVVSGEFAVSQKWVIDKVTILGLRKGTKINGYTVRTGAVTRKGD 875

Query: 893 NSSIRKTYQYFAKFMNVEISGLSIPIWEEFIL 915
            S ++ T     +F+  EISGL++ +  EF L
Sbjct: 876 KSKLKSTPDRKGEFIVAEISGLNLLLGREFKL 900

BLAST of CSPI01G34880 vs. ExPASy Swiss-Prot
Match: O04931 (Alpha-glucosidase OS=Beta vulgaris OX=161934 PE=1 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 4.7e-307
Identity = 499/878 (56.83%), Postives = 667/878 (75.97%), Query Frame = 0

Query: 52  AVGLGYRIRSSHVD-PAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDS 111
           A+G GY+++++ VD   GK+LTA L LI  S VYGPD+  L+  A+FE  D LR+R TD+
Sbjct: 40  AIGYGYQVKNAKVDNSTGKSLTALLQLIRNSPVYGPDIHFLSFTASFEEDDTLRIRFTDA 99

Query: 112 TRERWEIPHHILPRPS-----------SSLIRSLPENHVASPKASFISHPASDLIFTLHD 171
              RWEIP+ +LPRP              L + +P+N    P  + +SHP SDL FTL  
Sbjct: 100 NNRRWEIPNEVLPRPPPPPSPPPLSSLQHLPKPIPQN---QPTTTVLSHPHSDLAFTLFH 159

Query: 172 TAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRK 231
           T PFGF++ R+S+ DVLFD +P  S+  TFL++KDQY+QLSSSLP  ++ ++G+GE T+ 
Sbjct: 160 TTPFGFTIYRKSTHDVLFDATPIPSNPTTFLIYKDQYLQLSSSLPAQQAHLYGLGEHTKP 219

Query: 232 SFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNS 291
           +F+L    N+ LTLWNADI S N D+NLYG+HPFY+D+RS         G+THGV LLNS
Sbjct: 220 TFQLA--HNQILTLWNADIASFNRDLNLYGSHPFYMDVRSSPM-----VGSTHGVFLLNS 279

Query: 292 NGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRY 351
           NGMD+ Y+GDRITYKVIGGIIDLY FAG +P  V+DQYT+LIGRPAP+PYW+FGFHQCR+
Sbjct: 280 NGMDVEYTGDRITYKVIGGIIDLYIFAGRTPEMVLDQYTKLIGRPAPMPYWAFGFHQCRW 339

Query: 352 GYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKN 411
           GY++V++IE+VV +YA+A IPLE MWTDIDYMD +KDFT DP++FP  KM+ FV  LH+N
Sbjct: 340 GYRDVNEIETVVDKYAEARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVTKLHRN 399

Query: 412 GQKYVLILDPGISTNNTYGPYIRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETF 471
           GQ+YV ILDPGI+TN +YG +IRG ++++F+K NG PYLG VWPGPVY+PDF  P + +F
Sbjct: 400 GQRYVPILDPGINTNKSYGTFIRGMQSNVFIKRNGNPYLGSVWPGPVYYPDFLDPAARSF 459

Query: 472 WGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVP 531
           W  EI+ FRDI+P DG+WIDMNE SNFITS+ +P S LDNPPY INN+  + P+N+KT+P
Sbjct: 460 WVDEIKRFRDILPIDGIWIDMNEASNFITSAPTPGSTLDNPPYKINNSGGRVPINSKTIP 519

Query: 532 ASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDN 591
           A+ +H+GN+TEYN HNLYGFLES+AT  +LV+   + PF+LSRSTF GSGKYTAHWTGDN
Sbjct: 520 ATAMHYGNVTEYNAHNLYGFLESQATREALVRPATRGPFLLSRSTFAGSGKYTAHWTGDN 579

Query: 592 GATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDK 651
            A W+DL Y+IP++LNFGLFG+PM+G+DICGF+  TTEELC RWIQLGAFYPF+RDHS +
Sbjct: 580 AARWDDLQYSIPTMLNFGLFGMPMIGADICGFAESTTEELCCRWIQLGAFYPFSRDHSAR 639

Query: 652 GSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTH 711
            +  QELYLW+SVAASAR VL LRY+LLPY+YTLMY+A+ +G+PIARPL F+FP D+ T+
Sbjct: 640 DTTHQELYLWESVAASARTVLGLRYELLPYYYTLMYDANLRGSPIARPLSFTFPDDVATY 699

Query: 712 EIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADH 771
            I SQFL+G G++VSPVL+ G+  V+AY P GNW SL NY+  V+V++G  ++L AP DH
Sbjct: 700 GISSQFLIGRGIMVSPVLQPGSSIVNAYSPRGNWVSLSNYTSSVSVSAGTYVSLSAPPDH 759

Query: 772 INVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGN 831
           INVH+ EGNI+A+ GEAMTT+AA+ TP+ LLVV+S+  +S GE+FLD+G  +++G  GG 
Sbjct: 760 INVHIHEGNIVAMQGEAMTTQAARSTPFHLLVVMSDHVASTGELFLDNGIEMDIGGPGGK 819

Query: 832 WSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKG-L 891
           W++VRF++E+  + L + S+V+N G+A+SQ+ ++DK+T +G KR  K+ +  +    G +
Sbjct: 820 WTLVRFFAESGINNLTISSEVVNRGYAMSQRWVMDKITILGLKRRVKIKEYTVQKDAGAI 879

Query: 892 NLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFILEM 917
            + G      ++     F++V IS L   + + F LE+
Sbjct: 880 KVKGLGRRTSSHNQGGFFVSV-ISDLRQLVGQAFKLEL 906

BLAST of CSPI01G34880 vs. ExPASy Swiss-Prot
Match: Q653V7 (Probable alpha-glucosidase Os06g0675700 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0675700 PE=1 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 7.5e-289
Identity = 493/903 (54.60%), Postives = 650/903 (71.98%), Query Frame = 0

Query: 26  PFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIRSSHVDPAGKTLTADLDLI----GTS 85
           P    L  L +FL   FL  P      V  GY + S  V  +   L A L+L     G +
Sbjct: 8   PPARRLGALAVFLLALFLAAPW----GVDCGYNVAS--VAGSKNRLRARLELAGGGGGAA 67

Query: 86  QVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIRSLPENHVASP 145
              GPD+  L+L A+ E+  RL VRITD+   RWE+P  ++PRPS       P++ +A+ 
Sbjct: 68  PELGPDVRRLSLTASLETDSRLHVRITDADHPRWEVPQDVIPRPS-------PDSFLAAT 127

Query: 146 K---ASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKDQYIQ 205
           +      +S   SDL F +H T+PF F+V RRS+GDVLFDT+P        LVFKD+Y++
Sbjct: 128 RPGGGRVLSTATSDLTFAIH-TSPFRFTVTRRSTGDVLFDTTPN-------LVFKDRYLE 187

Query: 206 LSSSL-PKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDL 265
           L+SSL P  R+S++G+GEQT+++F+L   +N T TLWN+DI + N+D+NLYG+HPFY+D+
Sbjct: 188 LTSSLPPPGRASLYGLGEQTKRTFRL--QRNDTFTLWNSDIAAGNVDLNLYGSHPFYMDV 247

Query: 266 RSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQY 325
           RS    G    G  HGVLLLNSNGMD+IY G  +TYKVIGG++D YFFAGPSP++V+DQY
Sbjct: 248 RSGGGGG---GGAAHGVLLLNSNGMDVIYGGSYVTYKVIGGVLDFYFFAGPSPLAVVDQY 307

Query: 326 TELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDF 385
           T+LIGRPAP+PYWSFGFHQCRYGYKNV+D+E VVA YAKA IPLE MWTDIDYMD YKDF
Sbjct: 308 TQLIGRPAPMPYWSFGFHQCRYGYKNVADLEGVVAGYAKARIPLEVMWTDIDYMDAYKDF 367

Query: 386 TFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMKYNGVPY 445
           T DP+NFP+ +M+ FVD LH+NGQK+V+I+DPGI+ N TYG ++RG K DIF+K+NG  Y
Sbjct: 368 TLDPVNFPADRMRPFVDRLHRNGQKFVVIIDPGINVNTTYGTFVRGMKQDIFLKWNGSNY 427

Query: 446 LGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNL 505
           LG VWPG VYFPDF +P +  FW REI  FR  +P DGLW+DMNEISNF+     PL+ +
Sbjct: 428 LGVVWPGNVYFPDFLNPRAAEFWAREIAAFRRTLPVDGLWVDMNEISNFV--DPPPLNAI 487

Query: 506 DNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGQRP 565
           D+PPY INN+ V+RP+NNKTVPAS +H+G + EY+ HNL+GFLE+RATH +L++ TG+RP
Sbjct: 488 DDPPYRINNSGVRRPINNKTVPASAVHYGGVAEYDAHNLFGFLEARATHDALLRDTGRRP 547

Query: 566 FVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTE 625
           FVLSRSTFVGSG+YTAHWTGDN ATW DL Y+I ++L+FGLFGIPM+G+DICGF G+TTE
Sbjct: 548 FVLSRSTFVGSGRYTAHWTGDNAATWEDLHYSINTMLSFGLFGIPMIGADICGFGGNTTE 607

Query: 626 ELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEA 685
           ELC RWIQLGAFYPF+RDHS  G++R+ELYLW+SVA SARK L LRY+LLPY YTLMYEA
Sbjct: 608 ELCSRWIQLGAFYPFSRDHSAIGTVRRELYLWESVARSARKALGLRYRLLPYLYTLMYEA 667

Query: 686 HKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWFSLF 745
           H  G PIARPLFFS+P D++T+ ID QFLLG GVLVSPVL+ GA +V AYFPAG WFSL+
Sbjct: 668 HTTGAPIARPLFFSYPGDVETYGIDRQFLLGRGVLVSPVLEPGATTVTAYFPAGRWFSLY 727

Query: 746 NYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQ 805
           ++S  VA  +G+++ L APAD +NVHV  GNIL L   A+T+   +++   LLV +++  
Sbjct: 728 DFSLAVATKTGKRVTLPAPADTVNVHVAGGNILTLQQPALTSSRVRQSVVHLLVALADDG 787

Query: 806 SSFGEVFLDDGEVVEMGREGGNWSMVRF--YSETVGSKLVVKSQVINGGFALSQKLIIDK 865
           ++ G++FLDDGE  EM      WS ++F   +E+ G  + V+S V++  +A S+ + I K
Sbjct: 788 TATGDLFLDDGESPEMAGPRSRWSQIKFSGATESGGGVVRVRSHVVHDSYAPSRTMAIGK 847

Query: 866 VTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAK--FMNVEISGLSIPIWEEFI 917
           V  +G +         +  + G+ +N ++++        K       +SGL++ + +EF 
Sbjct: 848 VVLMGLRSAAPPKGFAV-YANGVQVNASTAVGGAAGSPEKGALGVAHVSGLTLVVGQEFD 881

BLAST of CSPI01G34880 vs. ExPASy Swiss-Prot
Match: Q43763 (Alpha-glucosidase OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 6.0e-278
Identity = 465/837 (55.56%), Postives = 608/837 (72.64%), Query Frame = 0

Query: 87  DLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPS-SSLIRSLPENHVASPKASF 146
           D+  L + A+ E+  RLRVRITD+   RWE+P  I+PRP+   ++   P    A  +   
Sbjct: 59  DVQRLAVYASLETDSRLRVRITDADHPRWEVPQDIIPRPAPGDVLHDAPPASSAPLQGRV 118

Query: 147 ISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKDQYIQLSSSLPK 206
           +S   SDL+ T+H  +PF F+V RRS+GD LFDT+P        LVF+D+Y++++S+LP 
Sbjct: 119 LSPAGSDLVLTVH-ASPFRFTVSRRSTGDTLFDTAPG-------LVFRDKYLEVTSALPA 178

Query: 207 DRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGK 266
            R+S++G+GE T+ SF+L    N + TLWNADIG+  +DVNLYG+HPFY+D+R+P     
Sbjct: 179 GRASLYGLGEHTKSSFRL--RHNDSFTLWNADIGASYVDVNLYGSHPFYMDVRAP----- 238

Query: 267 VAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 326
              GT HGVLLL+SNGMD++Y G  +TYKVIGG++D YFFAGP+P++V+DQYT+LI RPA
Sbjct: 239 ---GTAHGVLLLSSNGMDVLYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIARPA 298

Query: 327 PVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFP 386
           P+PYWSFGFHQCRYGY NVSD+E VVARYAKA IPLE MWTDIDYMDG+KDFT D +NF 
Sbjct: 299 PMPYWSFGFHQCRYGYLNVSDLERVVARYAKARIPLEVMWTDIDYMDGFKDFTLDRVNFT 358

Query: 387 SQKMKIFVDNLHKNGQKYVLILDPGIST---NNTYGPYIRGTKADIFMKYNGVPYLGDVW 446
           + +++ FVD LH+N QKYVLILDPGI     + TYG ++RG + DIF+K NG  ++G+VW
Sbjct: 359 AAELRPFVDRLHRNAQKYVLILDPGIRVDPIDATYGTFVRGMQQDIFLKRNGTNFVGNVW 418

Query: 447 PGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPY 506
           PG VYFPDF HP +  FW REI +FR  +P DGLWIDMNEISNF   +  P++ LD+PPY
Sbjct: 419 PGDVYFPDFMHPAAAEFWAREISLFRRTIPVDGLWIDMNEISNFY--NPEPMNALDDPPY 478

Query: 507 MINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGQRPFVLSR 566
            INN    RP+NNKTV    +H+G +TEY  HNL+G LE+RAT   +++ TG+RPFVLSR
Sbjct: 479 RINNDGTGRPINNKTVRPLAVHYGGVTEYEEHNLFGLLEARATGRGVLRDTGRRPFVLSR 538

Query: 567 STFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 626
           STFVGSG+YTA+WTGDN ATW DL Y+I ++L+FGLFG+PM+G+DICGF+G+TTEELC R
Sbjct: 539 STFVGSGRYTAYWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTEELCGR 598

Query: 627 WIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGT 686
           WIQLGAFYPF+RDHS   ++R+ELYLW SVAAS RK L LRYQLLPYFYTLMYEAH  G 
Sbjct: 599 WIQLGAFYPFSRDHSAIFTVRRELYLWPSVAASGRKALGLRYQLLPYFYTLMYEAHMTGA 658

Query: 687 PIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEF 746
           PIARPLFFS+P D+ T+ +D QFLLG GVLVSPVL+ G  +VDAYFPAG W+ L++YS  
Sbjct: 659 PIARPLFFSYPHDVATYGVDRQFLLGRGVLVSPVLEPGPTTVDAYFPAGRWYRLYDYSLA 718

Query: 747 VAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGE 806
           VA  +G+ + L APAD +NVH+  G IL L   A+TT  A+ T + LLV ++   ++ G 
Sbjct: 719 VATRTGKHVRLPAPADTVNVHLTGGTILPLQQSALTTSRARRTAFHLLVALAEDGTASGY 778

Query: 807 VFLDDGEVVEMGREGGNWSMVRFYSETVGSK--LVVKSQVINGGFALSQKLIIDKVTFVG 866
           +FLDDG+  E GR   +WSMVRF  +   +K  + VKS+V++  +A S+ L+I KV  +G
Sbjct: 779 LFLDDGDSPEYGRR-SDWSMVRFNYKIPNNKGAIKVKSEVVHNSYAQSRTLVISKVVLMG 838

Query: 867 FKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNV-EISGLSIPIWEEFILEM 917
            + P     L ++++    +  +SS    YQ       V  I GLS+ + EEF L++
Sbjct: 839 HRSPAAPKKLTVHVNSA-EVEASSSAGTRYQNAGGLGGVAHIGGLSLVVGEEFELKV 873

BLAST of CSPI01G34880 vs. ExPASy Swiss-Prot
Match: Q9S7Y7 (Alpha-xylosidase 1 OS=Arabidopsis thaliana OX=3702 GN=XYL1 PE=1 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 6.9e-234
Identity = 417/932 (44.74%), Postives = 593/932 (63.63%), Query Frame = 0

Query: 15  MASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIRSSHVDPAGKTLTAD 74
           MAS SS +    F  SL + ++  F+     P  S   +G GYR+ S    P G  +   
Sbjct: 1   MASSSSSL---AFSLSLLLALILCFS-----PTQSYKTIGKGYRLVSIEESPDGGFI-GY 60

Query: 75  LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIRSLP 134
           L +   +++YG D+ TL L    E+  RLRV ITD+ ++RWE+P+++LPR     +  + 
Sbjct: 61  LQVKQKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKVI 120

Query: 135 ENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKD 194
                SP  +      S+LIF+ + T PF F+V RRS+ + LF+T+ +       LVFKD
Sbjct: 121 GKSRKSP-ITVQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTTSS-------LVFKD 180

Query: 195 QYIQLSSSLPKDRSSIFGIGEQTR-KSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPF 254
           QY+++S+SLPK+ +S++G+GE ++    KLVP  N+  TL+  D+ ++NL+ +LYG+HP 
Sbjct: 181 QYLEISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINLNTDLYGSHPM 240

Query: 255 YIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISV 314
           Y+DLR+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGG+ D YF AGPSP++V
Sbjct: 241 YMDLRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNV 300

Query: 315 IDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDG 374
           +DQYT+LIGRPAP+PYWS GFHQCR+GY N+S +E VV  Y KA IPL+ +W D D+MDG
Sbjct: 301 VDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDG 360

Query: 375 YKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMKYN 434
           +KDFT +P+ +P  K+  F+D +HK G KY++I DPGI  N +YG + R   AD+F+KY 
Sbjct: 361 HKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYE 420

Query: 435 GVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFIT----- 494
           G P+L  VWPGPVYFPDF +P + ++WG EI+ F D+VP DGLWIDMNE+SNF +     
Sbjct: 421 GKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTI 480

Query: 495 -------SSTSP------------LSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLT 554
                  S   P             +  D+PPY IN   V  P+  KT+  S  H+  + 
Sbjct: 481 PEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVR 540

Query: 555 EYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYT 614
           EY+ H++YGF E+ ATH  L+ V G+RPF+LSRSTFVGSG+Y AHWTGDN  TW  L  +
Sbjct: 541 EYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVS 600

Query: 615 IPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLW 674
           I ++LNFG+FG+PMVGSDICGF    TEELC RWI++GAFYPF+RDH++  S RQELY W
Sbjct: 601 ISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQW 660

Query: 675 DSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGG 734
           D+VA SAR  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  + +    QFLLG 
Sbjct: 661 DTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGS 720

Query: 735 GVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNI 794
             ++SPVL++G   V+A FP G+W+ +F+ ++ V   +G+++ L AP + +NVH+ +  I
Sbjct: 721 SFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTI 780

Query: 795 LALHGEAMTTRAAQETPYKLLVVISNGQS---SFGEVFLDDGEVVEMGREGGNWSMVRFY 854
           L      + ++ A+ TP+ L++    G S   + G+++LD+ E+ EM    G  + V FY
Sbjct: 781 LPTQQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFY 840

Query: 855 SETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSI 913
           +      + + SQV  G FALS+  +I+KV+ +G +   ++ ++ +N S        SS 
Sbjct: 841 ASVGNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSK 900

BLAST of CSPI01G34880 vs. ExPASy TrEMBL
Match: A0A0A0M3Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G716250 PE=3 SV=1)

HSP 1 Score: 1880.5 bits (4870), Expect = 0.0e+00
Identity = 916/920 (99.57%), Postives = 920/920 (100.00%), Query Frame = 0

Query: 1   MDLTHTHFPLPQKTMASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIR 60
           MDLTHTHFPLPQKTMASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIR
Sbjct: 1   MDLTHTHFPLPQKTMASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIR 60

Query: 61  SSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHH 120
           SSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWE+PHH
Sbjct: 61  SSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEVPHH 120

Query: 121 ILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTS 180
           ILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTS
Sbjct: 121 ILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTS 180

Query: 181 PAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGS 240
           PAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGS
Sbjct: 181 PAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGS 240

Query: 241 VNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGII 300
           VNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDI+YSGDRITYKVIGGII
Sbjct: 241 VNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRITYKVIGGII 300

Query: 301 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIP 360
           DLYFFAGPSPISV+DQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIP
Sbjct: 301 DLYFFAGPSPISVVDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIP 360

Query: 361 LEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPY 420
           LEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPY
Sbjct: 361 LEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPY 420

Query: 421 IRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDM 480
           IRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDM
Sbjct: 421 IRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDM 480

Query: 481 NEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFL 540
           NEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFL
Sbjct: 481 NEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFL 540

Query: 541 ESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFG 600
           ESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFG
Sbjct: 541 ESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFG 600

Query: 601 IPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVL 660
           IPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVL
Sbjct: 601 IPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVL 660

Query: 661 ALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEG 720
           ALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEG
Sbjct: 661 ALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEG 720

Query: 721 AFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTR 780
           AFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTR
Sbjct: 721 AFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTR 780

Query: 781 AAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQV 840
           AAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQV
Sbjct: 781 AAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQV 840

Query: 841 INGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVE 900
           INGGFALSQKLIIDKVTFVGFKRP+KMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVE
Sbjct: 841 INGGFALSQKLIIDKVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVE 900

Query: 901 ISGLSIPIWEEFILEMTPIN 921
           ISGLSIPIWEEFILEMTPIN
Sbjct: 901 ISGLSIPIWEEFILEMTPIN 920

BLAST of CSPI01G34880 vs. ExPASy TrEMBL
Match: A0A5D3E1Q3 (Alpha-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00330 PE=3 SV=1)

HSP 1 Score: 1743.4 bits (4514), Expect = 0.0e+00
Identity = 855/909 (94.06%), Postives = 881/909 (96.92%), Query Frame = 0

Query: 15  MASGSSK--VIRTPFPHSLPILILFLFTSFL-PLPAASLPAVGLGYRIRSSHVDPAGKTL 74
           MASGSSK   IRTPF HSL ILILFLFTSFL P PAASLPAVG GYRIRSSH+DPAGK+L
Sbjct: 1   MASGSSKAITIRTPFAHSLLILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSL 60

Query: 75  TADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIR 134
           TADL LI TSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIP HI+PRPS+SLIR
Sbjct: 61  TADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIR 120

Query: 135 SLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLV 194
           SLPENHVASPK SFIS PASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSP FS+SETF+V
Sbjct: 121 SLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIV 180

Query: 195 FKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 254
           FKDQYIQLSS LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH
Sbjct: 181 FKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240

Query: 255 PFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 314
           PFYID+RS S+DGKV AGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI
Sbjct: 241 PFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300

Query: 315 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYM 374
           SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVS++ESVVARYAKASIPLE MWTDIDYM
Sbjct: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYM 360

Query: 375 DGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMK 434
           DGYKDFTFDPINFPS+KMKIFVDNLHKNGQKYV+ILDPGISTNNTYGPYIRGTKADIFMK
Sbjct: 361 DGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMK 420

Query: 435 YNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 494
           ++GVPYLGDVWPGPVYFPDF HPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST
Sbjct: 421 HDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480

Query: 495 SPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 554
           SPLSNLDNPPYMINNARV+RPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK
Sbjct: 481 SPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 540

Query: 555 VTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGF 614
           VTG+RPFVLSRSTF GSGKYTAHWTGD GATWND+GYTIPSILNFGLFGIPMVGSDICGF
Sbjct: 541 VTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGF 600

Query: 615 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 674
           SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY
Sbjct: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660

Query: 675 TLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAG 734
           TLMYEAHKKGTPIARPLFFSFPQDIKT+EIDSQFLLG GVLVSPVLKEGAFSVDAYFPAG
Sbjct: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAG 720

Query: 735 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLV 794
           NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTT+AA+ET YKLLV
Sbjct: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLV 780

Query: 795 VISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKL 854
           VISNGQ SFGEVFLDDGEV EMG EGGNWSMVRF SETVGSKLVVKSQVINGGFALSQKL
Sbjct: 781 VISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKL 840

Query: 855 IIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 914
           IIDKVTFVGF+RP+KMV LGLNISKG++LNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE
Sbjct: 841 IIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 900

Query: 915 FILEMTPIN 921
           FILEMTPI+
Sbjct: 901 FILEMTPIS 909

BLAST of CSPI01G34880 vs. ExPASy TrEMBL
Match: A0A1S3C8V0 (alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103498289 PE=3 SV=1)

HSP 1 Score: 1743.4 bits (4514), Expect = 0.0e+00
Identity = 855/909 (94.06%), Postives = 881/909 (96.92%), Query Frame = 0

Query: 15  MASGSSK--VIRTPFPHSLPILILFLFTSFL-PLPAASLPAVGLGYRIRSSHVDPAGKTL 74
           MASGSSK   IRTPF HSL ILILFLFTSFL P PAASLPAVG GYRIRSSH+DPAGK+L
Sbjct: 1   MASGSSKAITIRTPFAHSLLILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSL 60

Query: 75  TADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIR 134
           TADL LI TSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIP HI+PRPS+SLIR
Sbjct: 61  TADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIR 120

Query: 135 SLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLV 194
           SLPENHVASPK SFIS PASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSP FS+SETF+V
Sbjct: 121 SLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIV 180

Query: 195 FKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 254
           FKDQYIQLSS LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH
Sbjct: 181 FKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240

Query: 255 PFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 314
           PFYID+RS S+DGKV AGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI
Sbjct: 241 PFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300

Query: 315 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYM 374
           SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVS++ESVVARYAKASIPLE MWTDIDYM
Sbjct: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYM 360

Query: 375 DGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMK 434
           DGYKDFTFDPINFPS+KMKIFVDNLHKNGQKYV+ILDPGISTNNTYGPYIRGTKADIFMK
Sbjct: 361 DGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMK 420

Query: 435 YNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 494
           ++GVPYLGDVWPGPVYFPDF HPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST
Sbjct: 421 HDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480

Query: 495 SPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 554
           SPLSNLDNPPYMINNARV+RPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK
Sbjct: 481 SPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 540

Query: 555 VTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGF 614
           VTG+RPFVLSRSTF GSGKYTAHWTGD GATWND+GYTIPSILNFGLFGIPMVGSDICGF
Sbjct: 541 VTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGF 600

Query: 615 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 674
           SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY
Sbjct: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660

Query: 675 TLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAG 734
           TLMYEAHKKGTPIARPLFFSFPQDIKT+EIDSQFLLG GVLVSPVLKEGAFSVDAYFPAG
Sbjct: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAG 720

Query: 735 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLV 794
           NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTT+AA+ET YKLLV
Sbjct: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLV 780

Query: 795 VISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKL 854
           VISNGQ SFGEVFLDDGEV EMG EGGNWSMVRF SETVGSKLVVKSQVINGGFALSQKL
Sbjct: 781 VISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKL 840

Query: 855 IIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 914
           IIDKVTFVGF+RP+KMV LGLNISKG++LNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE
Sbjct: 841 IIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 900

Query: 915 FILEMTPIN 921
           FILEMTPI+
Sbjct: 901 FILEMTPIS 909

BLAST of CSPI01G34880 vs. ExPASy TrEMBL
Match: A0A6J1KFK1 (alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111495351 PE=3 SV=1)

HSP 1 Score: 1575.1 bits (4077), Expect = 0.0e+00
Identity = 771/925 (83.35%), Postives = 836/925 (90.38%), Query Frame = 0

Query: 9   PLPQKTMASGSSKV---IRTPFPHSLPILILFLFTSFLPLPAASLP----------AVGL 68
           P    TMA+GS  +   +R      L  ++LFLF+ F  L  ASLP          AVG 
Sbjct: 16  PYISTTMATGSKSLQAFLRR--RRLLSAVVLFLFSCFFRL-IASLPFAAGEQRENSAVGY 75

Query: 69  GYRIRSSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERW 128
           GYR+RS  VDP GK+LTADLDLI  S VYGPD+  L+LQA+FE+KDRLRVRITDS RERW
Sbjct: 76  GYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERW 135

Query: 129 EIPHHILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDV 188
           EIP  I+PR S+S IRSLPE  V SP+ S IS PASDLIF+LHDTAPFGFSV RRSSGDV
Sbjct: 136 EIPDEIIPRRSNSRIRSLPERDVGSPETSIISDPASDLIFSLHDTAPFGFSVHRRSSGDV 195

Query: 189 LFDTSPAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWN 248
           LFDTSP FSDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTRKSFK+VPDK+KTLTLW+
Sbjct: 196 LFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKIVPDKSKTLTLWS 255

Query: 249 ADIGSVNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKV 308
           ADIGSVN DVNLYGAHPFYID+RSPS+DGKVAAGTTHGVLLLNSNGMDIIYSGDRI+YK 
Sbjct: 256 ADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKA 315

Query: 309 IGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYA 368
           IGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVARYA
Sbjct: 316 IGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYA 375

Query: 369 KASIPLEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNN 428
           KA IPLE MWTDIDYMDGYKDFTFDPINFP +KMK FVD+LH+NGQKYVLI+DPGISTN 
Sbjct: 376 KAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDSLHQNGQKYVLIVDPGISTNK 435

Query: 429 TYGPYIRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDG 488
           TYG YIRG +ADIF+KY+GVPYLG+VWPGPVYFPDF HP+SE FWG EI++FRDIVPFDG
Sbjct: 436 TYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGGEIKLFRDIVPFDG 495

Query: 489 LWIDMNEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHN 548
           LWIDMNE+SNFITSSTSP SNLDNPPY I+NA VQRP+NN+TVPAS LHFGNLTEYNTHN
Sbjct: 496 LWIDMNELSNFITSSTSPFSNLDNPPYKIDNAGVQRPINNRTVPASSLHFGNLTEYNTHN 555

Query: 549 LYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILN 608
           LYGFLES+ATHASLVKVTG+RPFVLSRSTFVGSGKYTAHWTGDN ATWNDLGYTIPSILN
Sbjct: 556 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILN 615

Query: 609 FGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAAS 668
           FGLFGIPMVG+DICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWD+VAAS
Sbjct: 616 FGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAAS 675

Query: 669 ARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSP 728
           ARKVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+T+EI+SQFLLG GVLVSP
Sbjct: 676 ARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGEGVLVSP 735

Query: 729 VLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGE 788
           VLKEGA SVDAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVHVREGNILALHGE
Sbjct: 736 VLKEGALSVDAYFPAGNWFSLFNYSESVAVKSGQQITLDAPADHINVHVREGNILALHGE 795

Query: 789 AMTTRAAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLV 848
           A TT+AA++T +KLLVV+SNGQSS GEVFLDDGEVVEMG EGGNWS+VRFYSE VGSKL+
Sbjct: 796 ATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLL 855

Query: 849 VKSQVINGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAK 908
           +KSQVINGGFALSQK+IIDKVTFVGF+RP+KM  LGL+ISKG NLNGNS IRKTY+Y AK
Sbjct: 856 IKSQVINGGFALSQKMIIDKVTFVGFERPKKMGGLGLDISKGANLNGNSGIRKTYEYSAK 915

Query: 909 FMNVEISGLSIPIWEEFILEMTPIN 921
           F+NVEISGLSIPI EEF++E++P+N
Sbjct: 916 FVNVEISGLSIPICEEFVMELSPLN 937

BLAST of CSPI01G34880 vs. ExPASy TrEMBL
Match: A0A6J1ENT6 (alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111434234 PE=3 SV=1)

HSP 1 Score: 1561.6 bits (4042), Expect = 0.0e+00
Identity = 758/900 (84.22%), Postives = 819/900 (91.00%), Query Frame = 0

Query: 31  LPILILFLFTSFLPLPAASLP----------AVGLGYRIRSSHVDPAGKTLTADLDLIGT 90
           L  ++LFLFT F  L  ASLP          AVG GYR+RS  VDP GK+LTADLDLI  
Sbjct: 18  LSAVVLFLFTCFFRL-TASLPFAAGEQRENSAVGYGYRVRSGRVDPTGKSLTADLDLIDK 77

Query: 91  SQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIRSLPENHVAS 150
           S VYGPD+  L+LQA+FE+KDRLRVRITDS RERWE+P  I+PR S+S IRSLPE  V S
Sbjct: 78  SSVYGPDVERLSLQASFETKDRLRVRITDSNRERWEVPDEIIPRRSNSRIRSLPERDVGS 137

Query: 151 PKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKDQYIQLS 210
           P+ S IS PASDLIF+LHDTAPFGFSV RRSSGDVLFDTSP FSDSETFLVFKDQYIQLS
Sbjct: 138 PETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVLFDTSPNFSDSETFLVFKDQYIQLS 197

Query: 211 SSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSP 270
           SSLPKDRSS+FGIGEQTRKSFKLVPDK+KTLTLWNADIGSVN DVNLYGAHPFYID+RSP
Sbjct: 198 SSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNADIGSVNTDVNLYGAHPFYIDVRSP 257

Query: 271 SQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTEL 330
           S+DGKVAAGTTHGVLLLNSNGMDIIY+GDRI+YK IGGIIDLYFFAGPSPISVIDQYTEL
Sbjct: 258 SRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAIGGIIDLYFFAGPSPISVIDQYTEL 317

Query: 331 IGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFD 390
           IGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVARYAKA IPLE MWTDIDYMDGYKDFTFD
Sbjct: 318 IGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAKAGIPLEVMWTDIDYMDGYKDFTFD 377

Query: 391 PINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMKYNGVPYLGD 450
           PINFP +KMK FVDNLH+NGQKYVLILDPGISTN TYG YIRG +ADIF+KY+GVPYLG+
Sbjct: 378 PINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKTYGTYIRGIEADIFIKYDGVPYLGE 437

Query: 451 VWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNP 510
           VWPGPVYFPDF HP+SE FWG EI++ RDIVPFDGLWIDMNEISNFITSSTS  SNLDNP
Sbjct: 438 VWPGPVYFPDFLHPNSEIFWGDEIKLLRDIVPFDGLWIDMNEISNFITSSTSSFSNLDNP 497

Query: 511 PYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGQRPFVL 570
           PY INNA VQRP+NN+TVPAS LHFGNLTEYNTHNLYGFLES+ATHASLVKVTG+RPFVL
Sbjct: 498 PYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNLYGFLESKATHASLVKVTGKRPFVL 557

Query: 571 SRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELC 630
           SRSTFVGSGKYTAHWTGDN ATWNDLGYTIPSILNFGLFGIPMVG+DICGFSGDTTEELC
Sbjct: 558 SRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNFGLFGIPMVGADICGFSGDTTEELC 617

Query: 631 RRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKK 690
           RRWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY LLPY YTLMYEAHKK
Sbjct: 618 RRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYSLLPYIYTLMYEAHKK 677

Query: 691 GTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYS 750
           GTPIARPLFFSFPQDI+T+EI+SQFLLG GVL+SPVLKEGA SVDAYFPAGNWFSLFNYS
Sbjct: 678 GTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPVLKEGAISVDAYFPAGNWFSLFNYS 737

Query: 751 EFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSF 810
           E VA+ SGQQI LDAPADHINVHVREGNILALHGEA TT+AA++T +KLLVV+SNGQSS 
Sbjct: 738 ESVAIKSGQQITLDAPADHINVHVREGNILALHGEATTTQAARKTEFKLLVVVSNGQSSS 797

Query: 811 GEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVG 870
           GEVFLDDGE +EMG EGGNWS+VRFYSE VGSKL++KSQVINGGFALSQK+IIDKVT VG
Sbjct: 798 GEVFLDDGEEMEMGEEGGNWSLVRFYSEAVGSKLLIKSQVINGGFALSQKMIIDKVTIVG 857

Query: 871 FKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEEFILEMTPIN 921
           F+RP+ M DLGL+ISKG NLNGNS IR TY+Y AKF+NV+ISGLSIPI E F++E++ +N
Sbjct: 858 FERPKNMGDLGLDISKGANLNGNSGIRITYEYSAKFVNVQISGLSIPICEGFVMELSSLN 916

BLAST of CSPI01G34880 vs. NCBI nr
Match: XP_011660330.1 (alpha-glucosidase [Cucumis sativus] >KGN66921.1 hypothetical protein Csa_007658 [Cucumis sativus])

HSP 1 Score: 1880.5 bits (4870), Expect = 0.0e+00
Identity = 916/920 (99.57%), Postives = 920/920 (100.00%), Query Frame = 0

Query: 1   MDLTHTHFPLPQKTMASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIR 60
           MDLTHTHFPLPQKTMASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIR
Sbjct: 1   MDLTHTHFPLPQKTMASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIR 60

Query: 61  SSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHH 120
           SSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWE+PHH
Sbjct: 61  SSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEVPHH 120

Query: 121 ILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTS 180
           ILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTS
Sbjct: 121 ILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTS 180

Query: 181 PAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGS 240
           PAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGS
Sbjct: 181 PAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGS 240

Query: 241 VNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGII 300
           VNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDI+YSGDRITYKVIGGII
Sbjct: 241 VNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRITYKVIGGII 300

Query: 301 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIP 360
           DLYFFAGPSPISV+DQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIP
Sbjct: 301 DLYFFAGPSPISVVDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIP 360

Query: 361 LEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPY 420
           LEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPY
Sbjct: 361 LEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPY 420

Query: 421 IRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDM 480
           IRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDM
Sbjct: 421 IRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDM 480

Query: 481 NEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFL 540
           NEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFL
Sbjct: 481 NEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFL 540

Query: 541 ESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFG 600
           ESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFG
Sbjct: 541 ESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFG 600

Query: 601 IPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVL 660
           IPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVL
Sbjct: 601 IPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVL 660

Query: 661 ALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEG 720
           ALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEG
Sbjct: 661 ALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEG 720

Query: 721 AFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTR 780
           AFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTR
Sbjct: 721 AFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTR 780

Query: 781 AAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQV 840
           AAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQV
Sbjct: 781 AAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQV 840

Query: 841 INGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVE 900
           INGGFALSQKLIIDKVTFVGFKRP+KMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVE
Sbjct: 841 INGGFALSQKLIIDKVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVE 900

Query: 901 ISGLSIPIWEEFILEMTPIN 921
           ISGLSIPIWEEFILEMTPIN
Sbjct: 901 ISGLSIPIWEEFILEMTPIN 920

BLAST of CSPI01G34880 vs. NCBI nr
Match: XP_008459069.1 (PREDICTED: alpha-glucosidase [Cucumis melo] >KAA0043261.1 alpha-glucosidase [Cucumis melo var. makuwa] >TYK29325.1 alpha-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 1743.4 bits (4514), Expect = 0.0e+00
Identity = 855/909 (94.06%), Postives = 881/909 (96.92%), Query Frame = 0

Query: 15  MASGSSK--VIRTPFPHSLPILILFLFTSFL-PLPAASLPAVGLGYRIRSSHVDPAGKTL 74
           MASGSSK   IRTPF HSL ILILFLFTSFL P PAASLPAVG GYRIRSSH+DPAGK+L
Sbjct: 1   MASGSSKAITIRTPFAHSLLILILFLFTSFLIPPPAASLPAVGFGYRIRSSHIDPAGKSL 60

Query: 75  TADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIR 134
           TADL LI TSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIP HI+PRPS+SLIR
Sbjct: 61  TADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIPRPSNSLIR 120

Query: 135 SLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLV 194
           SLPENHVASPK SFIS PASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSP FS+SETF+V
Sbjct: 121 SLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDFSNSETFIV 180

Query: 195 FKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 254
           FKDQYIQLSS LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH
Sbjct: 181 FKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAH 240

Query: 255 PFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 314
           PFYID+RS S+DGKV AGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI
Sbjct: 241 PFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPI 300

Query: 315 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYM 374
           SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVS++ESVVARYAKASIPLE MWTDIDYM
Sbjct: 301 SVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEVMWTDIDYM 360

Query: 375 DGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMK 434
           DGYKDFTFDPINFPS+KMKIFVDNLHKNGQKYV+ILDPGISTNNTYGPYIRGTKADIFMK
Sbjct: 361 DGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRGTKADIFMK 420

Query: 435 YNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 494
           ++GVPYLGDVWPGPVYFPDF HPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST
Sbjct: 421 HDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSST 480

Query: 495 SPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 554
           SPLSNLDNPPYMINNARV+RPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK
Sbjct: 481 SPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVK 540

Query: 555 VTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGF 614
           VTG+RPFVLSRSTF GSGKYTAHWTGD GATWND+GYTIPSILNFGLFGIPMVGSDICGF
Sbjct: 541 VTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPMVGSDICGF 600

Query: 615 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 674
           SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY
Sbjct: 601 SGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFY 660

Query: 675 TLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAG 734
           TLMYEAHKKGTPIARPLFFSFPQDIKT+EIDSQFLLG GVLVSPVLKEGAFSVDAYFPAG
Sbjct: 661 TLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFSVDAYFPAG 720

Query: 735 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLV 794
           NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTT+AA+ET YKLLV
Sbjct: 721 NWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLV 780

Query: 795 VISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKL 854
           VISNGQ SFGEVFLDDGEV EMG EGGNWSMVRF SETVGSKLVVKSQVINGGFALSQKL
Sbjct: 781 VISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVINGGFALSQKL 840

Query: 855 IIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 914
           IIDKVTFVGF+RP+KMV LGLNISKG++LNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE
Sbjct: 841 IIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISGLSIPIWEE 900

Query: 915 FILEMTPIN 921
           FILEMTPI+
Sbjct: 901 FILEMTPIS 909

BLAST of CSPI01G34880 vs. NCBI nr
Match: XP_038894574.1 (alpha-glucosidase [Benincasa hispida])

HSP 1 Score: 1640.6 bits (4247), Expect = 0.0e+00
Identity = 805/913 (88.17%), Postives = 849/913 (92.99%), Query Frame = 0

Query: 15  MASGSSKVIRTPFPHSL------PILILFLFTSFLP-LPAASLPAVGLGYRIRSSHVDPA 74
           M SG SK +RTPF  SL      P L+ FLF SF P L A SL AVG GYRIRSSHVDPA
Sbjct: 1   MGSG-SKAMRTPFSQSLQPPLLFPALLFFLFPSFFPNLVAGSLSAVGYGYRIRSSHVDPA 60

Query: 75  GKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSS 134
           GK+LTADLDLI TS V GPD+P LTLQATFE+KDRLR+RITDSTRERWEIP  I+PR SS
Sbjct: 61  GKSLTADLDLIRTSPVNGPDVPRLTLQATFETKDRLRIRITDSTRERWEIPDQIIPRRSS 120

Query: 135 SLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSE 194
           S IRSLPENHV SP+ SFIS PASDLIFTLH TAPFGFSVLRRSSGDVLFDTSP  SDSE
Sbjct: 121 SRIRSLPENHVGSPRTSFISDPASDLIFTLHYTAPFGFSVLRRSSGDVLFDTSPDLSDSE 180

Query: 195 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNL 254
           TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTR+SFKL+PDKNKTLTLWNADIGSVNLDVNL
Sbjct: 181 TFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLMPDKNKTLTLWNADIGSVNLDVNL 240

Query: 255 YGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAG 314
           YGAHPFYID+RSPS DGKVAAGTTHGVLLLNSNGMDIIYSG RI YKVIGGIIDLYFFAG
Sbjct: 241 YGAHPFYIDVRSPSGDGKVAAGTTHGVLLLNSNGMDIIYSGHRIMYKVIGGIIDLYFFAG 300

Query: 315 PSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTD 374
           PSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSD+ESVVARYAKA IPLE MWTD
Sbjct: 301 PSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDLESVVARYAKAGIPLEVMWTD 360

Query: 375 IDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKAD 434
           IDYMDGYKDFTFDP+NFP++KMK FVDNLHKNGQKYVLILDPGISTNNTYG +IRG +AD
Sbjct: 361 IDYMDGYKDFTFDPVNFPAEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTFIRGKRAD 420

Query: 435 IFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFI 494
           IF++Y+GVPYLG+VWPGPVYFPDF HP+SE FWG EIQ+FRDIVPFDGLWIDMNEISNFI
Sbjct: 421 IFIQYDGVPYLGEVWPGPVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNFI 480

Query: 495 TSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHA 554
           TSSTSP SNLDNPPYMINNA V+RPLNNKTVPASILHFGNLTEYNTHNLYGFLES+ATHA
Sbjct: 481 TSSTSPFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESKATHA 540

Query: 555 SLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSD 614
           SLVKVTG+RPFVLSRSTFVGSGKYTAHWTGDNGATWNDL  TIPSILNFGLFGIPMVG+D
Sbjct: 541 SLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLRNTIPSILNFGLFGIPMVGAD 600

Query: 615 ICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLL 674
           ICGFSGDTTEELC RWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY+LL
Sbjct: 601 ICGFSGDTTEELCGRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRLL 660

Query: 675 PYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAY 734
           PYFYTLMYEAHKKGTPIARPLFFSFPQDIKT+ I+SQFLLG GVLVSPVLKEGA SVDAY
Sbjct: 661 PYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYGINSQFLLGEGVLVSPVLKEGAVSVDAY 720

Query: 735 FPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPY 794
           FP GNWFSLFNYSE VAV SGQ+I LDAPADHINVHVREGNILALHG+AMTTRAA+ET Y
Sbjct: 721 FPTGNWFSLFNYSELVAVKSGQRITLDAPADHINVHVREGNILALHGKAMTTRAARETAY 780

Query: 795 KLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFAL 854
           +LLVV+SN QSSFGEVFLDDGEVVEMG EGGNWS+VRFYSE VGSKLVVKSQVINGGFAL
Sbjct: 781 ELLVVVSNDQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEAVGSKLVVKSQVINGGFAL 840

Query: 855 SQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLSIP 914
           SQ LIIDKVTFVGF+RP+KMVDLGLNISKG+ LNGNS+IRKTY+YFAK MNVEISGLSIP
Sbjct: 841 SQNLIIDKVTFVGFERPKKMVDLGLNISKGVKLNGNSTIRKTYKYFAKVMNVEISGLSIP 900

Query: 915 IWEEFILEMTPIN 921
           IWEEF+LEMTPI+
Sbjct: 901 IWEEFMLEMTPIS 912

BLAST of CSPI01G34880 vs. NCBI nr
Match: XP_023520110.1 (alpha-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1577.4 bits (4083), Expect = 0.0e+00
Identity = 773/928 (83.30%), Postives = 835/928 (89.98%), Query Frame = 0

Query: 6   THFPLPQKTMASGSSKV---IRTPFPHSLPILILFLFTSFLPLPAASLP----------A 65
           TH P    TMA+GS  +   +R      L  ++LFLFT F  L  ASLP          A
Sbjct: 14  TH-PYSSTTMATGSKSLQAFLRR--RRLLSAVVLFLFTCFFRL-TASLPFAAGEQRENSA 73

Query: 66  VGLGYRIRSSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTR 125
           VG GYR+RS  VDP GK+LTADLDLI  S VYGPD+  L++QA+FE+KDRLRVRITDS R
Sbjct: 74  VGYGYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNR 133

Query: 126 ERWEIPHHILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSS 185
           ERWE+P  I+PR S+S IRSLPE  V SP+ S IS PASDLIF+LHDTAPFGFSV RRSS
Sbjct: 134 ERWEVPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSS 193

Query: 186 GDVLFDTSPAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLT 245
           GDVLFDTSP FSDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTRKSFKLVPDK+KTLT
Sbjct: 194 GDVLFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLT 253

Query: 246 LWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRIT 305
           LWNADIGSVN DVNLYGAHPFYID+RSPS+DGKVAAGTTHGVLLLNSNGMDIIYSGDRI+
Sbjct: 254 LWNADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRIS 313

Query: 306 YKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVA 365
           YK IGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVA
Sbjct: 314 YKAIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVA 373

Query: 366 RYAKASIPLEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGIS 425
           RYAKA IPLE MWTDIDYMDGYKDFTFDPINFP +KMK FVDNLH+NGQKYVLILDPGIS
Sbjct: 374 RYAKAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGIS 433

Query: 426 TNNTYGPYIRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVP 485
           TN TYG YIRG +ADIF+KY+GVPYLG+VWPGPVYFPDF HP+SE FWG EI++FRDIVP
Sbjct: 434 TNKTYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVP 493

Query: 486 FDGLWIDMNEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYN 545
           FDGLWIDMNEISNFITSSTS  SNLDNPPY INNA VQRP+NN+TVPAS LHFGNLTEYN
Sbjct: 494 FDGLWIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYN 553

Query: 546 THNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPS 605
           THNLYGFLES+ATHASLVKVTG+RPFVLSRSTFVGSGKYTAHWTGDN ATWNDLGYTIPS
Sbjct: 554 THNLYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPS 613

Query: 606 ILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSV 665
           ILNFGLFGIPMVG+DICGFS DTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWD+V
Sbjct: 614 ILNFGLFGIPMVGADICGFSRDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTV 673

Query: 666 AASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVL 725
           AASARKVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+T+EI+SQFLLG GVL
Sbjct: 674 AASARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVL 733

Query: 726 VSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILAL 785
           +SPVLKEGA SVDAYFPAGNWFSLFNYSE VAVNSGQQI LDAPADHINVHVREGNILAL
Sbjct: 734 ISPVLKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILAL 793

Query: 786 HGEAMTTRAAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGS 845
           HGEA TT+AA++T +KLLVV+SNGQSS GEVFLDDGEVVEMG EGGNWS+VRFYSE VGS
Sbjct: 794 HGEATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGS 853

Query: 846 KLVVKSQVINGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQY 905
           KL++KSQVINGGFALSQK+IIDKVT VGF+RP+ M  LGL+ISKG NL+GNS IRKTY+Y
Sbjct: 854 KLLIKSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEY 913

Query: 906 FAKFMNVEISGLSIPIWEEFILEMTPIN 921
            AKF+NVEISGLSI IWEEF++E++P+N
Sbjct: 914 SAKFVNVEISGLSISIWEEFVMELSPLN 937

BLAST of CSPI01G34880 vs. NCBI nr
Match: XP_023001112.1 (alpha-glucosidase [Cucurbita maxima])

HSP 1 Score: 1575.1 bits (4077), Expect = 0.0e+00
Identity = 771/925 (83.35%), Postives = 836/925 (90.38%), Query Frame = 0

Query: 9   PLPQKTMASGSSKV---IRTPFPHSLPILILFLFTSFLPLPAASLP----------AVGL 68
           P    TMA+GS  +   +R      L  ++LFLF+ F  L  ASLP          AVG 
Sbjct: 16  PYISTTMATGSKSLQAFLRR--RRLLSAVVLFLFSCFFRL-IASLPFAAGEQRENSAVGY 75

Query: 69  GYRIRSSHVDPAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERW 128
           GYR+RS  VDP GK+LTADLDLI  S VYGPD+  L+LQA+FE+KDRLRVRITDS RERW
Sbjct: 76  GYRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERW 135

Query: 129 EIPHHILPRPSSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDV 188
           EIP  I+PR S+S IRSLPE  V SP+ S IS PASDLIF+LHDTAPFGFSV RRSSGDV
Sbjct: 136 EIPDEIIPRRSNSRIRSLPERDVGSPETSIISDPASDLIFSLHDTAPFGFSVHRRSSGDV 195

Query: 189 LFDTSPAFSDSETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWN 248
           LFDTSP FSDSETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTRKSFK+VPDK+KTLTLW+
Sbjct: 196 LFDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKIVPDKSKTLTLWS 255

Query: 249 ADIGSVNLDVNLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKV 308
           ADIGSVN DVNLYGAHPFYID+RSPS+DGKVAAGTTHGVLLLNSNGMDIIYSGDRI+YK 
Sbjct: 256 ADIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKA 315

Query: 309 IGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYA 368
           IGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVARYA
Sbjct: 316 IGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYA 375

Query: 369 KASIPLEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNN 428
           KA IPLE MWTDIDYMDGYKDFTFDPINFP +KMK FVD+LH+NGQKYVLI+DPGISTN 
Sbjct: 376 KAGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDSLHQNGQKYVLIVDPGISTNK 435

Query: 429 TYGPYIRGTKADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDG 488
           TYG YIRG +ADIF+KY+GVPYLG+VWPGPVYFPDF HP+SE FWG EI++FRDIVPFDG
Sbjct: 436 TYGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGGEIKLFRDIVPFDG 495

Query: 489 LWIDMNEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHN 548
           LWIDMNE+SNFITSSTSP SNLDNPPY I+NA VQRP+NN+TVPAS LHFGNLTEYNTHN
Sbjct: 496 LWIDMNELSNFITSSTSPFSNLDNPPYKIDNAGVQRPINNRTVPASSLHFGNLTEYNTHN 555

Query: 549 LYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILN 608
           LYGFLES+ATHASLVKVTG+RPFVLSRSTFVGSGKYTAHWTGDN ATWNDLGYTIPSILN
Sbjct: 556 LYGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILN 615

Query: 609 FGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAAS 668
           FGLFGIPMVG+DICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWD+VAAS
Sbjct: 616 FGLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAAS 675

Query: 669 ARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSP 728
           ARKVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+T+EI+SQFLLG GVLVSP
Sbjct: 676 ARKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGEGVLVSP 735

Query: 729 VLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGE 788
           VLKEGA SVDAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVHVREGNILALHGE
Sbjct: 736 VLKEGALSVDAYFPAGNWFSLFNYSESVAVKSGQQITLDAPADHINVHVREGNILALHGE 795

Query: 789 AMTTRAAQETPYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLV 848
           A TT+AA++T +KLLVV+SNGQSS GEVFLDDGEVVEMG EGGNWS+VRFYSE VGSKL+
Sbjct: 796 ATTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLL 855

Query: 849 VKSQVINGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSIRKTYQYFAK 908
           +KSQVINGGFALSQK+IIDKVTFVGF+RP+KM  LGL+ISKG NLNGNS IRKTY+Y AK
Sbjct: 856 IKSQVINGGFALSQKMIIDKVTFVGFERPKKMGGLGLDISKGANLNGNSGIRKTYEYSAK 915

Query: 909 FMNVEISGLSIPIWEEFILEMTPIN 921
           F+NVEISGLSIPI EEF++E++P+N
Sbjct: 916 FVNVEISGLSIPICEEFVMELSPLN 937

BLAST of CSPI01G34880 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 557/897 (62.10%), Postives = 697/897 (77.70%), Query Frame = 0

Query: 27  FPHSLPILILF--LFTSFLPLPAASLPAVGLGYRIRSSHVDPAGKTLTADLDLIGTSQVY 86
           FP+   ++++F  L +S + L       VG GY +RS  VD   + LTA LDLI  S VY
Sbjct: 7   FPNIFIVVVVFFSLRSSQVVLEEEESTVVGYGYVVRSVGVDSNRQVLTAKLDLIKPSSVY 66

Query: 87  GPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIR--SLPENHVASPK 146
            PD+ +L L  + E+ +RLR+RITDS+++RWEIP  ++PR  +   R  S  E+   SP+
Sbjct: 67  APDIKSLNLHVSLETSERLRIRITDSSQQRWEIPETVIPRAGNHSPRRFSTEEDGGNSPE 126

Query: 147 ASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKDQYIQLSSS 206
            +F++ P+SDL+FTLH+T PFGFSV RRSSGD+LFDTSP  SDS T+ +FKDQ++QLSS+
Sbjct: 127 NNFLADPSSDLVFTLHNTTPFGFSVSRRSSGDILFDTSPDSSDSNTYFIFKDQFLQLSSA 186

Query: 207 LPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQ 266
           LP++RS+++GIGE T++SF+L+P   +T+TLWNADIGS N DVNLYG+HPFY+D+R    
Sbjct: 187 LPENRSNLYGIGEHTKRSFRLIP--GETMTLWNADIGSENPDVNLYGSHPFYMDVR--GS 246

Query: 267 DGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIG 326
            G   AGTTHGVLLLNSNGMD+ Y G RITY VIGG+IDLY FAGPSP  V++QYTELIG
Sbjct: 247 KGNEEAGTTHGVLLLNSNGMDVKYEGHRITYNVIGGVIDLYVFAGPSPEMVMNQYTELIG 306

Query: 327 RPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPI 386
           RPAP+PYWSFGFHQCRYGYKNVSD+E VV  YAKA IPLE MWTDIDYMDGYKDFT DP+
Sbjct: 307 RPAPMPYWSFGFHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPV 366

Query: 387 NFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMKYNGVPYLGDVW 446
           NFP  KM+ FVD LHKNGQKYVLILDPGI  +++YG Y RG +AD+F+K NG PYLG+VW
Sbjct: 367 NFPEDKMQSFVDTLHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFIKRNGEPYLGEVW 426

Query: 447 PGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPY 506
           PG VYFPDF +P + TFW  EI++F++I+P DGLWIDMNE+SNFITS  S  S+LD+PPY
Sbjct: 427 PGKVYFPDFLNPAAATFWSNEIKMFQEILPLDGLWIDMNELSNFITSPLSSGSSLDDPPY 486

Query: 507 MINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGQRPFVLSR 566
            INN+  +RP+NNKTVPA+ +HFGN++EY+ HNLYG LE++ATH ++V +TG+RPF+LSR
Sbjct: 487 KINNSGDKRPINNKTVPATSIHFGNISEYDAHNLYGLLEAKATHQAVVDITGKRPFILSR 546

Query: 567 STFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 626
           STFV SGKYTAHWTGDN A W DL Y+IP ILNFGLFGIPMVG+DICGFS DTTEELCRR
Sbjct: 547 STFVSSGKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELCRR 606

Query: 627 WIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGT 686
           WIQLGAFYPFARDHS  G+ RQELYLWDSVA+SARKVL LR +LLP+ YTLMYEAH  G 
Sbjct: 607 WIQLGAFYPFARDHSSLGTARQELYLWDSVASSARKVLGLRMRLLPHLYTLMYEAHVSGN 666

Query: 687 PIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEF 746
           PIARPLFFSFPQD KT+EIDSQFL+G  ++VSP LK+GA +VDAYFPAGNWF LFNYS  
Sbjct: 667 PIARPLFFSFPQDTKTYEIDSQFLIGKSIMVSPALKQGAVAVDAYFPAGNWFDLFNYSFA 726

Query: 747 VAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGE 806
           V  +SG+ + LD PADH+NVHVREG+I+A+ GEA+TTR A++TPY+LLVV S  ++  GE
Sbjct: 727 VGGDSGKHVRLDTPADHVNVHVREGSIVAMQGEALTTRDARKTPYQLLVVASRLENISGE 786

Query: 807 VFLDDGEVVEMGREGGN--WSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVG 866
           +FLDDGE + MG  GGN  W++V+F     G  +V++S+V+N  +A   K  I KVTFVG
Sbjct: 787 LFLDDGENLRMGAGGGNRDWTLVKFRCYVTGKSVVLRSEVVNPEYASKMKWSIGKVTFVG 846

Query: 867 FKRPRKMVDLGLNISKGLNLNGNSSIRKTYQY-FAKFMNVEISGLSIPIWEEFILEM 917
           F+    +    +  S+ L     S I+        +F++VE+S LS+ + ++F + +
Sbjct: 847 FENVENVKTYEVRTSERLRSPRISLIKTVSDNDDPRFLSVEVSKLSLLVGKKFEMRL 899

BLAST of CSPI01G34880 vs. TAIR 10
Match: AT1G68560.1 (alpha-xylosidase 1 )

HSP 1 Score: 812.0 bits (2096), Expect = 4.9e-235
Identity = 417/932 (44.74%), Postives = 593/932 (63.63%), Query Frame = 0

Query: 15  MASGSSKVIRTPFPHSLPILILFLFTSFLPLPAASLPAVGLGYRIRSSHVDPAGKTLTAD 74
           MAS SS +    F  SL + ++  F+     P  S   +G GYR+ S    P G  +   
Sbjct: 1   MASSSSSL---AFSLSLLLALILCFS-----PTQSYKTIGKGYRLVSIEESPDGGFI-GY 60

Query: 75  LDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIRSLP 134
           L +   +++YG D+ TL L    E+  RLRV ITD+ ++RWE+P+++LPR     +  + 
Sbjct: 61  LQVKQKNKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKVI 120

Query: 135 ENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETFLVFKD 194
                SP  +      S+LIF+ + T PF F+V RRS+ + LF+T+ +       LVFKD
Sbjct: 121 GKSRKSP-ITVQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTTSS-------LVFKD 180

Query: 195 QYIQLSSSLPKDRSSIFGIGEQTR-KSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPF 254
           QY+++S+SLPK+ +S++G+GE ++    KLVP  N+  TL+  D+ ++NL+ +LYG+HP 
Sbjct: 181 QYLEISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINLNTDLYGSHPM 240

Query: 255 YIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISV 314
           Y+DLR+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGG+ D YF AGPSP++V
Sbjct: 241 YMDLRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNV 300

Query: 315 IDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDG 374
           +DQYT+LIGRPAP+PYWS GFHQCR+GY N+S +E VV  Y KA IPL+ +W D D+MDG
Sbjct: 301 VDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDG 360

Query: 375 YKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMKYN 434
           +KDFT +P+ +P  K+  F+D +HK G KY++I DPGI  N +YG + R   AD+F+KY 
Sbjct: 361 HKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYE 420

Query: 435 GVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFIT----- 494
           G P+L  VWPGPVYFPDF +P + ++WG EI+ F D+VP DGLWIDMNE+SNF +     
Sbjct: 421 GKPFLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTI 480

Query: 495 -------SSTSP------------LSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLT 554
                  S   P             +  D+PPY IN   V  P+  KT+  S  H+  + 
Sbjct: 481 PEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVR 540

Query: 555 EYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYT 614
           EY+ H++YGF E+ ATH  L+ V G+RPF+LSRSTFVGSG+Y AHWTGDN  TW  L  +
Sbjct: 541 EYDAHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVS 600

Query: 615 IPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLW 674
           I ++LNFG+FG+PMVGSDICGF    TEELC RWI++GAFYPF+RDH++  S RQELY W
Sbjct: 601 ISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQW 660

Query: 675 DSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGG 734
           D+VA SAR  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  + +    QFLLG 
Sbjct: 661 DTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGS 720

Query: 735 GVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNI 794
             ++SPVL++G   V+A FP G+W+ +F+ ++ V   +G+++ L AP + +NVH+ +  I
Sbjct: 721 SFMISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTI 780

Query: 795 LALHGEAMTTRAAQETPYKLLVVISNGQS---SFGEVFLDDGEVVEMGREGGNWSMVRFY 854
           L      + ++ A+ TP+ L++    G S   + G+++LD+ E+ EM    G  + V FY
Sbjct: 781 LPTQQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFY 840

Query: 855 SETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFKRPRKMVDLGLNISKGLNLNGNSSI 913
           +      + + SQV  G FALS+  +I+KV+ +G +   ++ ++ +N S        SS 
Sbjct: 841 ASVGNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSK 900

BLAST of CSPI01G34880 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 794.7 bits (2051), Expect = 8.1e-230
Identity = 410/895 (45.81%), Postives = 567/895 (63.35%), Query Frame = 0

Query: 30  SLPILILFLFTSFLPLPAASLPAVGLGYRIRSSHVDPAGKTLTADLDLIGTSQVYGPDLP 89
           SL + I+  F+S          A+G GYR+ S    P   +    L +  ++++YG D+ 
Sbjct: 6   SLLVAIILCFSSL-----QCSNAIGKGYRLISMEKSPDDGSFIGYLQVKQSNKIYGSDIT 65

Query: 90  TLTLQATFESKDRLRVRITDSTRERWEIPHHILPRPSSSLIRSLPENHVASPKASFISHP 149
            L L   + +  RLRV ITD+ ++RWE+P+++L R     +  + ++  +      IS P
Sbjct: 66  ILRLFINYRTDHRLRVHITDAKKQRWEVPYNLLRREQPPNV--IGKSRKSPVTVQEISGP 125

Query: 150 ASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSDSETF--LVFKDQYIQLSSSLPKDR 209
              LIFT+    PF F+V RRS+G+ +F+TS   S  E+F  +VFKDQY+++S+SLPKD 
Sbjct: 126 ELILIFTVD---PFSFAVRRRSNGETIFNTS---SSDESFGEMVFKDQYLEISTSLPKD- 185

Query: 210 SSIFGIGEQTR-KSFKLVPDKNKTLTLWNADIGSVNLDVNLYGAHPFYIDLRSPSQDGKV 269
           +S++G GE ++    KLVP  N+  TL+  D+ + NL+ +LYG+HP Y+DLR+ S  GK 
Sbjct: 186 ASLYGFGENSQANGIKLVP--NEPYTLFTEDVSAFNLNTDLYGSHPVYMDLRNVS--GKA 245

Query: 270 AAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAP 329
            A   H VLLLNS+GMD+ Y GD +TYKVIGG+ D YFFAGPSP++V+DQYT LIGRPAP
Sbjct: 246 YA---HSVLLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGPSPLNVVDQYTSLIGRPAP 305

Query: 330 VPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFPS 389
           +PYWS GFHQCR+GY+NVS ++ VV  Y KA IPL+ +W D DYMDGYKDFT D +NFP 
Sbjct: 306 MPYWSLGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYMDGYKDFTLDLVNFPH 365

Query: 390 QKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFMKYNGVPYLGDVWPGPV 449
            K+  F+D +HK G KYV+I DPGI  N +YG Y RG  +D+F+KY G P+L  VWPGPV
Sbjct: 366 AKLLSFLDRIHKMGMKYVVIKDPGIGVNASYGVYQRGMASDVFIKYEGKPFLAQVWPGPV 425

Query: 450 YFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNFITSSTSPLSNLDNPPYMINN 509
           YFPDF +P + ++WG EI+ F ++VP DGLWIDMNE                     IN 
Sbjct: 426 YFPDFLNPKTVSWWGDEIRRFHELVPIDGLWIDMNE---------------------INA 485

Query: 510 ARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASLVKVTGQRPFVLSRSTFV 569
              +  L  KT+P S  H+  + EY+ H++YGF E+ ATH +L+ V G+RPF+LSRSTFV
Sbjct: 486 TGHKASLGFKTIPTSAYHYNGVREYDAHSIYGFSEAIATHKALLAVQGKRPFILSRSTFV 545

Query: 570 GSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRRWIQL 629
           GSG+Y AHWTGDN  TW  L  +I ++LNFG+FG+PMVGSDICGF   T EELC RWI++
Sbjct: 546 GSGQYAAHWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELCNRWIEV 605

Query: 630 GAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQLLPYFYTLMYEAHKKGTPIAR 689
           GAFYPF+RDH+D  + R+ELY W +VA SAR  L +RY+LLP+ YTL YEAH  G PIAR
Sbjct: 606 GAFYPFSRDHADYYAPRKELYQWGTVAESARNALGMRYKLLPFLYTLNYEAHMSGAPIAR 665

Query: 690 PLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVDAYFPAGNWFSLFNYSEFVAVN 749
           PLFFSFP+  + + +  QFLLG  +++SPVL++G   V+A FP G+W+ +F+ ++ V   
Sbjct: 666 PLFFSFPEFTECYGLSKQFLLGSSLMISPVLEQGKTQVEALFPPGSWYHMFDMTQVVVSK 725

Query: 750 SGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQETPYKLLVVISNGQSSFGEVFLD 809
           +G+   L AP + +NVH+ +  IL +         A E           G +S G++FLD
Sbjct: 726 NGRLFTLPAPFNVVNVHLYQNAILPMQQVVAFPAGASE-----------GYAS-GKLFLD 785

Query: 810 DGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGFALSQKLIIDKVTFVGFKRPRK 869
           D E+ EM    G  + + FY+      + + SQV  G FALSQ L+I+KV  +G K   K
Sbjct: 786 DDELPEMKLGNGKSTYIDFYASVGNESVKIWSQVKEGQFALSQGLVIEKVIVLGLKGTWK 845

Query: 870 MVDLGLNISKGLNLNGNSSIRKTYQYF---------AKFMNVEISGLSIPIWEEF 913
           + ++ LN S   N      +    Q +         +K   VE+ GL + + ++F
Sbjct: 846 VSEILLNGSSISNETKTIEVSSKEQMYVVGSEDEGESKSFMVELKGLEMLVGKDF 846

BLAST of CSPI01G34880 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 327.8 bits (839), Expect = 2.8e-89
Identity = 223/689 (32.37%), Postives = 332/689 (48.19%), Query Frame = 0

Query: 206 DRSSIFGIGEQTRKSFKLVPDK------NKTLTLWNADIGSVNLD--VNLYGAHPFYIDL 265
           D S ++GI E    SF L P K      ++   L+N D+   + +    LYG+ PF +  
Sbjct: 222 DSSFVYGIPEHA-TSFALKPTKGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMV-- 281

Query: 266 RSPSQDGKVAAGTTHGVLLLNS---------------NGMDIIYSGDRIT--YKVIGGII 325
                 GK  +G T G   LN+               +G+ +  S  RI   +    GI+
Sbjct: 282 ----SHGK--SGKTSGFFWLNAAEMQIDVLANGWDAESGISLPSSHSRIDTFWMSEAGIV 341

Query: 326 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIP 385
           D +FF GP P  V+ QY  + G  A    ++ G+HQCR+ YK+  D+  V +++ +  IP
Sbjct: 342 DTFFFVGPEPKDVVKQYASVTGTSAMPQLFATGYHQCRWNYKDEEDVAQVDSKFDEHDIP 401

Query: 386 LEAMWTDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPY 445
            + +W DI++ DG + FT+D + FP  +       L   G+K V I+DP I  +++Y  +
Sbjct: 402 YDVLWLDIEHTDGKRYFTWDSVLFPHPEE--MQKKLAAKGRKMVTIVDPHIKRDDSYFLH 461

Query: 446 IRGTKADIFMK-YNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQI--FRDIVPFDGLW 505
              T+   ++K  +G  + G  WPG   + D   P+   +WG       +    P    W
Sbjct: 462 KEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRFSYKNYVGSTPSLYTW 521

Query: 506 IDMNEISNFITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLY 565
            DMNE                  P + N   V       T+P   LH G +     HN Y
Sbjct: 522 NDMNE------------------PSVFNGPEV-------TMPRDALHVGGVEHREVHNAY 581

Query: 566 GFLESRATHASLV--KVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILN 625
           G+    AT   LV  +    RPFVLSR+ F G+ +Y A WTGDN A W  L  +IP IL 
Sbjct: 582 GYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMILT 641

Query: 626 FGLFGIPMVGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLW-DSVAA 685
            GL GI   G+DI GF G+   EL  RW Q+GA+YPF R H+   + R+E +L+ +    
Sbjct: 642 LGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNTE 701

Query: 686 SARKVLALRYQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVS 745
             R  +  RY LLPYFYTL  EA+  G P+ RPL+  FPQD  T   D  F++G G+LV 
Sbjct: 702 LMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSGLLVQ 761

Query: 746 PVLKEGAFSVDAYFPA-GNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALH 805
            V  +G      Y P   +W+ L N   +V    G+   +DAP + I    + G I+   
Sbjct: 762 GVYTKGTTQASVYLPGKESWYDLRNGKTYV---GGKTHKMDAPEESIPAFQKAGTIIPRK 821

Query: 806 GEAMTTRAAQET-PYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGS 861
                + +  +  PY L+V +++ Q + GE+++DDG+  E  R  G++   RF    V S
Sbjct: 822 DRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEFRR--GSYIHRRF----VFS 865

BLAST of CSPI01G34880 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 306.2 bits (783), Expect = 8.8e-83
Identity = 197/644 (30.59%), Postives = 318/644 (49.38%), Query Frame = 0

Query: 208 SSIFGIGEQTRKSFKLVPDKNKTLTLWNADI-GSVNLDVNLYGAHPFYIDLRSPSQDGKV 267
           +S +G GE + +    +    K +  WN D  G  +   +LY +HP+ + +    +   V
Sbjct: 86  TSFYGTGEVSGQ----LERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGV 145

Query: 268 AAGTTHGVLL-LNSNGMDIIYSGDRITYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 327
            A TT    + L   G+  I S    +Y +I       F    SP +V++  +  IG   
Sbjct: 146 LADTTRKCEIDLRKEGIIRIIS--PASYPII------TFGPFSSPTAVLESLSHAIGTVF 205

Query: 328 PVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMWTDIDYMDGYKDFTFDPINFP 387
             P W+ G+HQCR+ Y +   +  +   +    IP + +W DIDYMDG++ FTFD   FP
Sbjct: 206 MPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFP 265

Query: 388 SQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTKADIFM-KYNGVPYLGDVWPG 447
                    +LH NG K + +LDPGI     Y  Y  G+K D+++ + +G P+ G+VWPG
Sbjct: 266 DP--SALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPG 325

Query: 448 PVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISNF-ITSSTSPLSNLDNPPYM 507
           P  FPD+ +  + ++W   ++ F      DG+W DMNE + F + + T P +N+ +    
Sbjct: 326 PCVFPDYTNSKARSWWANLVKEFVS-NGVDGIWNDMNEPAVFKVVTKTMPENNIHHGDDE 385

Query: 508 INNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRATHASL-VKVTGQRPFVLSR 567
           +   +                  N + Y  HN+YG L +R+T+  + +    +RPFVL+R
Sbjct: 386 LGGVQ------------------NHSHY--HNVYGMLMARSTYEGMELADKNKRPFVLTR 445

Query: 568 STFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVGSDICGFSGDTTEELCRR 627
           + F+GS +Y A WTGDN + W  L  +I  +L  GL G P+ G DI GF+G+ T  L  R
Sbjct: 446 AGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGR 505

Query: 628 WIQLGAFYPFARDHSDKGSIRQELYLW-DSVAASARKVLALRYQLLPYFYTLMYEAHKKG 687
           W+ +GA +PF R HS+ G+   E + + +      R  L  RYQLLP+FYTL Y AH  G
Sbjct: 506 WMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTG 565

Query: 688 TPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVL-KEGAFSVDAYFPAGNWFSLFNYS 747
            P+A P+FF+ P D +   +++ FLLG  ++ +  L  +G+  +    P G W   F+++
Sbjct: 566 APVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHR-FDFA 625

Query: 748 EFVAVNSGQQINLDAPADHINVHVREGNILALHGEAM-TTRAAQETPYKLLVVISNGQSS 807
                        D+  D   ++++ G+I++L    +     +      LLV +     +
Sbjct: 626 -------------DSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKA 678

Query: 808 FGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVING 844
            G +F DDG+    G   G + +  + +E   S + VK     G
Sbjct: 686 KGLLFEDDGD--GYGYTKGRFLVTHYIAERDSSTVTVKVSKTEG 678