CSPI01G34220 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G34220
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Subtilisin-like protease SBT2.4
Location	Chr1: 29112581 .. 29113144 (+)
RNA-Seq Expression	CSPI01G34220
Synteny	CSPI01G34220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGATGATGGTGGTGATGAACTAAGAAGCAACAAAGAAATGTTGTTAATAGAAGATTCACACAAGAAGGTTTTAGAAAACAGTATAAAAAACTACAGAAAGCTGCATAGCTTCAAGAAAATTTTGAATGGTTTTGCAGTCCACACAACACCATCAGAAGCTTCAAAGCTCAGAGAAGCTAATGGAGTGAAATTGGTTGAGTTAGATAGAGGAGTGAGGAAAATGACGACTTATACCCCTGAGTTTTTAGGGTTAGTGAAGAATAATAATGATTATAAATATAATTATAGTGGTGGTGGGGATGGGATTTTGATTGGGTTTGTGGATTCTGGGATTTATCCAACACATCCAAGTTTTAGCAATAACTTTGGTAAAGAAGATGATGATGAGTTGGTTTGTGAAGAAGGTCCTTTATTTCCAAAAGGTTGTTGCAATGGGAAGATAGTGAGTGCTAGGTTTTTCTCTGCTGGGGCTCAGGCTGCTGCAGTGCTTAATTCTTCTATTGATTTCCTATCTCCATTTGATGCTGAAGGCCATGGAAGGTATCCGATCCTAGCTTAG

mRNA sequence

ATGGAAGATGATGGTGGTGATGAACTAAGAAGCAACAAAGAAATGTTGTTAATAGAAGATTCACACAAGAAGGTTTTAGAAAACAGTATAAAAAACTACAGAAAGCTGCATAGCTTCAAGAAAATTTTGAATGGTTTTGCAGTCCACACAACACCATCAGAAGCTTCAAAGCTCAGAGAAGCTAATGGAGTGAAATTGGTTGAGTTAGATAGAGGAGTGAGGAAAATGACGACTTATACCCCTGAGTTTTTAGGGTTAGTGAAGAATAATAATGATTATAAATATAATTATAGTGGTGGTGGGGATGGGATTTTGATTGGGTTTGTGGATTCTGGGATTTATCCAACACATCCAAGTTTTAGCAATAACTTTGGTAAAGAAGATGATGATGAGTTGGTTTGTGAAGAAGGTCCTTTATTTCCAAAAGGTTGTTGCAATGGGAAGATAGTGAGTGCTAGGTTTTTCTCTGCTGGGGCTCAGGCTGCTGCAGTGCTTAATTCTTCTATTGATTTCCTATCTCCATTTGATGCTGAAGGCCATGGAAGGTATCCGATCCTAGCTTAG

Coding sequence (CDS)

ATGGAAGATGATGGTGGTGATGAACTAAGAAGCAACAAAGAAATGTTGTTAATAGAAGATTCACACAAGAAGGTTTTAGAAAACAGTATAAAAAACTACAGAAAGCTGCATAGCTTCAAGAAAATTTTGAATGGTTTTGCAGTCCACACAACACCATCAGAAGCTTCAAAGCTCAGAGAAGCTAATGGAGTGAAATTGGTTGAGTTAGATAGAGGAGTGAGGAAAATGACGACTTATACCCCTGAGTTTTTAGGGTTAGTGAAGAATAATAATGATTATAAATATAATTATAGTGGTGGTGGGGATGGGATTTTGATTGGGTTTGTGGATTCTGGGATTTATCCAACACATCCAAGTTTTAGCAATAACTTTGGTAAAGAAGATGATGATGAGTTGGTTTGTGAAGAAGGTCCTTTATTTCCAAAAGGTTGTTGCAATGGGAAGATAGTGAGTGCTAGGTTTTTCTCTGCTGGGGCTCAGGCTGCTGCAGTGCTTAATTCTTCTATTGATTTCCTATCTCCATTTGATGCTGAAGGCCATGGAAGGTATCCGATCCTAGCTTAG

Protein sequence

MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRYPILA*

Homology

[Close]

BLAST of CSPI01G34220 vs. ExPASy Swiss-Prot
Match: F4HYR6 (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana OX=3702 GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.0e-39
Identity = 91/184 (49.46%), Postives = 119/184 (64.67%), Query Frame = 0


Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           IE+ H ++L ++++  +Y KL+SFK ++N  AV TT S+A KL +  GVK VE D+GV+ 
Sbjct: 78  IEEIHDEILGSTLEKGSYTKLYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKL 137

Query: 78  MTTYTPEFLGL-------VKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF-------- 137
           MTTYTP+FL L       + N  D +      G+ I+IGFVD+GI PTHPSF        
Sbjct: 138 MTTYTPDFLELPQQVWQKISNEGDRR-----AGEDIVIGFVDTGINPTHPSFAALDLTNP 197

Query: 138 -SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEG 184
            S+N  +       CE GP FP G CNGKI+SARFFSAGA+A+  LNSS+D LSPFDA G
Sbjct: 198 YSSNLSRLHFSG-DCEIGPFFPPGSCNGKIISARFFSAGARASGALNSSLDILSPFDASG 255

[Close]

BLAST of CSPI01G34220 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.8e-33
Identity = 71/157 (45.22%), Postives = 99/157 (63.06%), Query Frame = 0


Query: 32  NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNN 91
           +Y+KL+S+K ++NGFA H +P +A  LR A GVK V+ D  VRK+TT+TP+FLGL  +  
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLGLPTDVW 141

Query: 92  DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNN-----FGKEDDDELVCEEGPLFPKGCCN 151
                Y   G+ I+IGF+DSGI+P HPSF+++     +G     +  CEE P      CN
Sbjct: 142 PTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHTKISFCN 201

Query: 152 GKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           GKI+ A+ F+  A+AA   N  IDF SP D +GHG +
Sbjct: 202 GKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSH 238

[Close]

BLAST of CSPI01G34220 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.8e-32
Identity = 71/156 (45.51%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 32  NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNN 91
           +Y+KL+S+K ++NGFA H +P +A  LR A GV+ V+ D  VR++TT+TPEFLGL  +  
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTDVW 141

Query: 92  DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNN----FGKEDDDELVCEEGPLFPKGCCNG 151
                +   G+ I+IGFVDSGIYP HPSF+++    +G     +  CEE P   K  CN 
Sbjct: 142 PTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKKSFCNR 201

Query: 152 KIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           KIV A+ F+  A+AA   N  ID+ SP D +GHG +
Sbjct: 202 KIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSH 237

[Close]

BLAST of CSPI01G34220 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 7.7e-31
Identity = 73/174 (41.95%), Postives = 99/174 (56.90%), Query Frame = 0


Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           I  +H  +L N++K   Y KL+SF  ++NGFAV  +  +A  L     V  + LD  VR 
Sbjct: 99  IAQAHDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRT 158

Query: 78  MTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDDEL--- 137
            TTYTP+F+GL K     +  Y   G+GI+IGF+D+GI PTHPSF+     +    +   
Sbjct: 159 ATTYTPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNH 218

Query: 138 ---VCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
              VCE  P FP G CN K+V AR F+  A    + NSS D+ SPFD +GHG +
Sbjct: 219 FSGVCEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTH 272

[Close]

BLAST of CSPI01G34220 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.5e-29
Identity = 68/173 (39.31%), Postives = 97/173 (56.07%), Query Frame = 0


Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           +  SH   L  ++K   Y KL+S+  ++NGFA+     +A KL     V  + LD  VR 
Sbjct: 86  VVQSHDSFLRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRT 145

Query: 78  MTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKED-----DD 137
            TTYTP+F+GL +     +  +   G+G++IGF+D+GI P HPSF++N  K         
Sbjct: 146 ATTYTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHF 205

Query: 138 ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
             VCE  P FP G CN K++ AR F+  A    + NSS D+ SPFD +GHG +
Sbjct: 206 SGVCEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTH 258

[Close]

BLAST of CSPI01G34220 vs. ExPASy TrEMBL
Match: A0A0A0LYI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701240 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.4e-99
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120
           ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF
Sbjct: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120

Query: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180
           SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH
Sbjct: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180

Query: 181 GRY 184
           G +
Sbjct: 181 GSH 183

[Close]

BLAST of CSPI01G34220 vs. ExPASy TrEMBL
Match: A0A5D3CKG6 (Subtilisin-like protease SBT2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00790 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.1e-91
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGG ELR++KEML IEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 1   MEDDGGYELRNSKEMLSIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSG-----GGDGILIGFVDSGIYP 120
           ANGVKLVELDRGV+KMTTYTPEFLGLVKNNNDYKYNYSG     GGDGILIGFVDSGIYP
Sbjct: 61  ANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGISGAGGDGILIGFVDSGIYP 120

Query: 121 THPSFSNNFGKEDDD--ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLS 180
            HPSFSNNFGK+DDD  ELVCEEGPLFPKGCCNGKIVSA FFSAGAQAAAVLNSSIDFLS
Sbjct: 121 KHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFFSAGAQAAAVLNSSIDFLS 180

Query: 181 PFDAEGHGRY 184
           PFDAEGHG +
Sbjct: 181 PFDAEGHGSH 190

[Close]

BLAST of CSPI01G34220 vs. ExPASy TrEMBL
Match: A0A5A7TIJ4 (Subtilisin-like protease SBT2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold110G00140 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.1e-91
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGG ELR++KEML IEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 1   MEDDGGYELRNSKEMLSIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSG-----GGDGILIGFVDSGIYP 120
           ANGVKLVELDRGV+KMTTYTPEFLGLVKNNNDYKYNYSG     GGDGILIGFVDSGIYP
Sbjct: 61  ANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGISGAGGDGILIGFVDSGIYP 120

Query: 121 THPSFSNNFGKEDDD--ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLS 180
            HPSFSNNFGK+DDD  ELVCEEGPLFPKGCCNGKIVSA FFSAGAQAAAVLNSSIDFLS
Sbjct: 121 KHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFFSAGAQAAAVLNSSIDFLS 180

Query: 181 PFDAEGHGRY 184
           PFDAEGHG +
Sbjct: 181 PFDAEGHGSH 190

[Close]

BLAST of CSPI01G34220 vs. ExPASy TrEMBL
Match: A0A1S3C9G9 (LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4 OS=Cucumis melo OX=3656 GN=LOC103498334 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.1e-91
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGG ELR++KEML IEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 25  MEDDGGYELRNSKEMLSIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 84

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSG-----GGDGILIGFVDSGIYP 120
           ANGVKLVELDRGV+KMTTYTPEFLGLVKNNNDYKYNYSG     GGDGILIGFVDSGIYP
Sbjct: 85  ANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGISGAGGDGILIGFVDSGIYP 144

Query: 121 THPSFSNNFGKEDDD--ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLS 180
            HPSFSNNFGK+DDD  ELVCEEGPLFPKGCCNGKIVSA FFSAGAQAAAVLNSSIDFLS
Sbjct: 145 KHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFFSAGAQAAAVLNSSIDFLS 204

Query: 181 PFDAEGHGRY 184
           PFDAEGHG +
Sbjct: 205 PFDAEGHGSH 214

[Close]

BLAST of CSPI01G34220 vs. ExPASy TrEMBL
Match: A0A6J1JWQ4 (subtilisin-like protease SBT2.4 OS=Cucurbita maxima OX=3661 GN=LOC111490380 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 5.3e-51
Identity = 110/173 (63.58%), Postives = 133/173 (76.88%), Query Frame = 0


Query: 15  MLLIEDSHKK--VLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRG 74
           ++L+E+S  +  +L+N++K+Y KL SFK I+NGFAVHTTPSEA+KLR+ANGVKLVE DRG
Sbjct: 56  LVLMEESSNEDHLLQNTLKSYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDRG 115

Query: 75  VRKMTTYTPEFLGLVKNNN--DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDD 134
           VRKMTTYTP+FLG+   +     K   S  G+GI+IGFVDSGI PTHPSF  + G E D 
Sbjct: 116 VRKMTTYTPKFLGVPSKSRLCSKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFDDGYE-DL 175

Query: 135 ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
            L CE+G  FP   CNGKIV ARFFSAGA+A A LNSS+DFLSPFD EGHG +
Sbjct: 176 RLFCEKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSH 227

[Close]

BLAST of CSPI01G34220 vs. NCBI nr
Match: XP_004145469.3 (subtilisin-like protease SBT2.4 [Cucumis sativus])

HSP 1 Score: 371.3 bits (952), Expect = 4.9e-99
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 32  MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 91

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120
           ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF
Sbjct: 92  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 151

Query: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180
           SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH
Sbjct: 152 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 211

Query: 181 GRY 184
           G +
Sbjct: 212 GSH 214

[Close]

BLAST of CSPI01G34220 vs. NCBI nr
Match: KAE8653651.1 (hypothetical protein Csa_006839 [Cucumis sativus])

HSP 1 Score: 371.3 bits (952), Expect = 4.9e-99
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 297 MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 356

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 120
           ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF
Sbjct: 357 ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF 416

Query: 121 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 180
           SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH
Sbjct: 417 SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGH 476

Query: 181 GRY 184
           G +
Sbjct: 477 GSH 479

[Close]

BLAST of CSPI01G34220 vs. NCBI nr
Match: XP_008459133.1 (PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4, partial [Cucumis melo])

HSP 1 Score: 344.4 bits (882), Expect = 6.4e-91
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGG ELR++KEML IEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 25  MEDDGGYELRNSKEMLSIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 84

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSG-----GGDGILIGFVDSGIYP 120
           ANGVKLVELDRGV+KMTTYTPEFLGLVKNNNDYKYNYSG     GGDGILIGFVDSGIYP
Sbjct: 85  ANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGISGAGGDGILIGFVDSGIYP 144

Query: 121 THPSFSNNFGKEDDD--ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLS 180
            HPSFSNNFGK+DDD  ELVCEEGPLFPKGCCNGKIVSA FFSAGAQAAAVLNSSIDFLS
Sbjct: 145 KHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFFSAGAQAAAVLNSSIDFLS 204

Query: 181 PFDAEGHGRY 184
           PFDAEGHG +
Sbjct: 205 PFDAEGHGSH 214

[Close]

BLAST of CSPI01G34220 vs. NCBI nr
Match: TYK12311.1 (subtilisin-like protease SBT2.4 [Cucumis melo var. makuwa])

HSP 1 Score: 344.4 bits (882), Expect = 6.4e-91
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGG ELR++KEML IEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 1   MEDDGGYELRNSKEMLSIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSG-----GGDGILIGFVDSGIYP 120
           ANGVKLVELDRGV+KMTTYTPEFLGLVKNNNDYKYNYSG     GGDGILIGFVDSGIYP
Sbjct: 61  ANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGISGAGGDGILIGFVDSGIYP 120

Query: 121 THPSFSNNFGKEDDD--ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLS 180
            HPSFSNNFGK+DDD  ELVCEEGPLFPKGCCNGKIVSA FFSAGAQAAAVLNSSIDFLS
Sbjct: 121 KHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFFSAGAQAAAVLNSSIDFLS 180

Query: 181 PFDAEGHGRY 184
           PFDAEGHG +
Sbjct: 181 PFDAEGHGSH 190

[Close]

BLAST of CSPI01G34220 vs. NCBI nr
Match: KAA0043154.1 (subtilisin-like protease SBT2.4 [Cucumis melo var. makuwa])

HSP 1 Score: 344.4 bits (882), Expect = 6.4e-91
Identity = 173/190 (91.05%), Postives = 178/190 (93.68%), Query Frame = 0


Query: 1   MEDDGGDELRSNKEMLLIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60
           MEDDGG ELR++KEML IEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE
Sbjct: 1   MEDDGGYELRNSKEMLSIEDSHKKVLENSIKNYRKLHSFKKILNGFAVHTTPSEASKLRE 60

Query: 61  ANGVKLVELDRGVRKMTTYTPEFLGLVKNNNDYKYNYSG-----GGDGILIGFVDSGIYP 120
           ANGVKLVELDRGV+KMTTYTPEFLGLVKNNNDYKYNYSG     GGDGILIGFVDSGIYP
Sbjct: 61  ANGVKLVELDRGVKKMTTYTPEFLGLVKNNNDYKYNYSGGISGAGGDGILIGFVDSGIYP 120

Query: 121 THPSFSNNFGKEDDD--ELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLS 180
            HPSFSNNFGK+DDD  ELVCEEGPLFPKGCCNGKIVSA FFSAGAQAAAVLNSSIDFLS
Sbjct: 121 KHPSFSNNFGKDDDDYGELVCEEGPLFPKGCCNGKIVSAMFFSAGAQAAAVLNSSIDFLS 180

Query: 181 PFDAEGHGRY 184
           PFDAEGHG +
Sbjct: 181 PFDAEGHGSH 190

[Close]

BLAST of CSPI01G34220 vs. TAIR 10
Match: AT1G62340.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 163.7 bits (413), Expect = 1.4e-40
Identity = 91/184 (49.46%), Postives = 119/184 (64.67%), Query Frame = 0


Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           IE+ H ++L ++++  +Y KL+SFK ++N  AV TT S+A KL +  GVK VE D+GV+ 
Sbjct: 78  IEEIHDEILGSTLEKGSYTKLYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKL 137

Query: 78  MTTYTPEFLGL-------VKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSF-------- 137
           MTTYTP+FL L       + N  D +      G+ I+IGFVD+GI PTHPSF        
Sbjct: 138 MTTYTPDFLELPQQVWQKISNEGDRR-----AGEDIVIGFVDTGINPTHPSFAALDLTNP 197

Query: 138 -SNNFGKEDDDELVCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEG 184
            S+N  +       CE GP FP G CNGKI+SARFFSAGA+A+  LNSS+D LSPFDA G
Sbjct: 198 YSSNLSRLHFSG-DCEIGPFFPPGSCNGKIISARFFSAGARASGALNSSLDILSPFDASG 255

[Close]

BLAST of CSPI01G34220 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 143.3 bits (360), Expect = 2.0e-34
Identity = 71/157 (45.22%), Postives = 99/157 (63.06%), Query Frame = 0


Query: 32  NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNN 91
           +Y+KL+S+K ++NGFA H +P +A  LR A GVK V+ D  VRK+TT+TP+FLGL  +  
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLGLPTDVW 141

Query: 92  DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNN-----FGKEDDDELVCEEGPLFPKGCCN 151
                Y   G+ I+IGF+DSGI+P HPSF+++     +G     +  CEE P      CN
Sbjct: 142 PTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHTKISFCN 201

Query: 152 GKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           GKI+ A+ F+  A+AA   N  IDF SP D +GHG +
Sbjct: 202 GKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSH 238

[Close]

BLAST of CSPI01G34220 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 140.6 bits (353), Expect = 1.3e-33
Identity = 71/156 (45.51%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 32  NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRKMTTYTPEFLGLVKNNN 91
           +Y+KL+S+K ++NGFA H +P +A  LR A GV+ V+ D  VR++TT+TPEFLGL  +  
Sbjct: 82  SYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTDVW 141

Query: 92  DYKYNYSGGGDGILIGFVDSGIYPTHPSFSNN----FGKEDDDELVCEEGPLFPKGCCNG 151
                +   G+ I+IGFVDSGIYP HPSF+++    +G     +  CEE P   K  CN 
Sbjct: 142 PTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKKSFCNR 201

Query: 152 KIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
           KIV A+ F+  A+AA   N  ID+ SP D +GHG +
Sbjct: 202 KIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSH 237

[Close]

BLAST of CSPI01G34220 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 135.2 bits (339), Expect = 5.5e-32
Identity = 73/174 (41.95%), Postives = 99/174 (56.90%), Query Frame = 0


Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           I  +H  +L N++K   Y KL+SF  ++NGFAV  +  +A  L     V  + LD  VR 
Sbjct: 99  IAQAHDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRT 158

Query: 78  MTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDDEL--- 137
            TTYTP+F+GL K     +  Y   G+GI+IGF+D+GI PTHPSF+     +    +   
Sbjct: 159 ATTYTPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNH 218

Query: 138 ---VCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
              VCE  P FP G CN K+V AR F+  A    + NSS D+ SPFD +GHG +
Sbjct: 219 FSGVCEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTH 272

[Close]

BLAST of CSPI01G34220 vs. TAIR 10
Match: AT4G20430.2 (Subtilase family protein )

HSP 1 Score: 135.2 bits (339), Expect = 5.5e-32
Identity = 73/174 (41.95%), Postives = 99/174 (56.90%), Query Frame = 0


Query: 18  IEDSHKKVLENSIK--NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVRK 77
           I  +H  +L N++K   Y KL+SF  ++NGFAV  +  +A  L     V  + LD  VR 
Sbjct: 99  IAQAHDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRT 158

Query: 78  MTTYTPEFLGLVKNNNDYKYNYSGGGDGILIGFVDSGIYPTHPSFSNNFGKEDDDEL--- 137
            TTYTP+F+GL K     +  Y   G+GI+IGF+D+GI PTHPSF+     +    +   
Sbjct: 159 ATTYTPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNH 218

Query: 138 ---VCEEGPLFPKGCCNGKIVSARFFSAGAQAAAVLNSSIDFLSPFDAEGHGRY 184
              VCE  P FP G CN K+V AR F+  A    + NSS D+ SPFD +GHG +
Sbjct: 219 FSGVCEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTH 272

The following BLAST results are available for this feature:

BLAST of CSPI01G34220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HYR6	2.0e-39	49.46	Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana OX=3702 GN=SBT2.4 PE=1 S...	[more]
Q9SZV5	2.8e-33	45.22	Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 S...	[more]
O64481	1.8e-32	45.51	Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 S...	[more]
Q9SUN6	7.7e-31	41.95	Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 S...	[more]
Q9FI12	5.5e-29	39.31	Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 S...	[more]

BLAST of CSPI01G34220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYI5	2.4e-99	98.91	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G701240 PE=3 SV=1	[more]
A0A5D3CKG6	3.1e-91	91.05	Subtilisin-like protease SBT2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A5A7TIJ4	3.1e-91	91.05	Subtilisin-like protease SBT2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A1S3C9G9	3.1e-91	91.05	LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4 OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1JWQ4	5.3e-51	63.58	subtilisin-like protease SBT2.4 OS=Cucurbita maxima OX=3661 GN=LOC111490380 PE=3...	[more]

BLAST of CSPI01G34220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145469.3	4.9e-99	98.91	subtilisin-like protease SBT2.4 [Cucumis sativus]	[more]
KAE8653651.1	4.9e-99	98.91	hypothetical protein Csa_006839 [Cucumis sativus]	[more]
XP_008459133.1	6.4e-91	91.05	PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4, partial [Cucumi...	[more]
TYK12311.1	6.4e-91	91.05	subtilisin-like protease SBT2.4 [Cucumis melo var. makuwa]	[more]
KAA0043154.1	6.4e-91	91.05	subtilisin-like protease SBT2.4 [Cucumis melo var. makuwa]	[more]

BLAST of CSPI01G34220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G62340.1	1.4e-40	49.46	PA-domain containing subtilase family protein	[more]
AT4G30020.1	2.0e-34	45.22	PA-domain containing subtilase family protein	[more]
AT2G19170.1	1.3e-33	45.51	subtilisin-like serine protease 3	[more]
AT4G20430.1	5.5e-32	41.95	Subtilase family protein	[more]
AT4G20430.2	5.5e-32	41.95	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 12..74 e-value: 1.2E-11 score: 45.1
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 6..74 e-value: 1.1E-5 score: 27.8
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 77..183 e-value: 7.0E-12 score: 47.0
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 58..181
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 18..182
None	No IPR available	PANTHER	PTHR10795:SF384	SUBTILISIN-LIKE PROTEASE SBT2.6	coord: 18..182
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 81..187 score: 8.976961
IPR023827	Peptidase S8, subtilisin, Asp-active site	PROSITE	PS00136	SUBTILASE_ASP	coord: 106..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G34220.1	CSPI01G34220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090558	plant epidermis development
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016020	membrane
molecular_function	GO:0004252	serine-type endopeptidase activity