CSPI01G33690 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G33690
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionmitochondrial import inner membrane translocase subunit TIM23-2
LocationChr1: 28638742 .. 28640219 (-)
RNA-Seq ExpressionCSPI01G33690
SyntenyCSPI01G33690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTCGATGAAAAAGGTATTAATTATTTTTATTATTTTCTATATTTTAGCTCTTTGTTTTTAGTTCCGCGTTAAACCCTACGCTACTCACGAAGCTTCCTCTCCCATAAACCCTTTCAAACCCTTTGAAGCTTTTTGAAGAAAGAAAGAGAAACCATTTCTTTCTCTTTCTCGCCATTGTTTTCTCTCTCTCCAACCTTTGATTCAACTCACCCTAAACCCTAATTTCTGTTCCTTCAAAACCTCCCTTTCTCCTCCTCCCATGGAAAATTCCTCCAAATCACCCGATGATCGGAAATCCCGCTTCTATCACCCTTACCAAGACCTACACGTTCCGATCACCAAGCTCTACGAGCTCCCCACCGCCCCCGAGCATCTTTTCTTCGAAGAAGCCGCCAGGCCTCACCGTTCTTGGGGCGAAAACCTCCAATATTACACCGGAATTGGCTACCTCTCCGGCGCCCTTTTTGGCGGCGCCAGGGGTTCCATTCAGGGCCTCAGGGCAGCTGAGCCTGGTGACTCTGTCAAACTCCGTCTCAATCGAGTTCTCAACTCTGGGGGCCAGCTTGGCCGGAGGGCTGGAAACTCTCTTGGGATCCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTATTCATTTGAGAGGTTCTGATGATGTTTTAAACAGTATTGTAGCTGGATTGGGGACTGGTGCTGTTTATAAGGCGGCGTCTGGGCCAAGGTCGGCTGCCATCGCCGGTGCTATTGGAGGAATTGCTGCTGCAGCTGCAGTTGCGGGTAAGCAGGCAGTTAAGAGATATGTGCCGATATAGAATTGGCATAACAAGTTATGCAAATTTGTACTAACTAGAGGGAGGATTTGCACGGTAAAGCATTTGGTTTCTTTGATTATTATTTCTTCATATTGTTATACAGTTAGTTTATTATTCATGGATTAGTGAATAATACACTTTTGGGTTGCAAATGCTCGCTCTTGTTATTTGTAATTGTTATTCATTTTGTTGATTTGCCTCTTATAGTTATTGATGCCCTGGATCAATGGGAGACTTTAAATTGGTGTTTGATTAATCTCTTCTTATGTGTAACTGACTCTGAAGCCTTCGTAATTATCTTGCCTGTGTTGGATCTCATTACAATGTAGCCATAGTACAGTATTGGAAAAAAGACTTTGCATAGATGACTGATTTCTTTATAATTTGTGGTTTATTGGAAAGTAGGGGATGGGTTTGGTGGTGACTTATTGTTGGGAGGGTTCATCTGAATCAATGAATAATACCAACGGAGTTTGTGGTAGCAGCCATTATTTTCTGTGTTATAGGCTAAAAGTGCAGGGGTTTCTTAGTTATATTGGCGAAGTTTCCGGAGGCCTAAATTTTGTCAAATAAGCCATTTTTCTTTACCAATACAATAGTCTTGGAAGTCTTGTCAAATATGGCGCTTAGAGTAGTTTTCCAAAGGTAGATATTGGTGGGGGGAAT

mRNA sequence

GCTCGATGAAAAAGGTATTAATTATTTTTATTATTTTCTATATTTTAGCTCTTTGTTTTTAGTTCCGCGTTAAACCCTACGCTACTCACGAAGCTTCCTCTCCCATAAACCCTTTCAAACCCTTTGAAGCTTTTTGAAGAAAGAAAGAGAAACCATTTCTTTCTCTTTCTCGCCATTGTTTTCTCTCTCTCCAACCTTTGATTCAACTCACCCTAAACCCTAATTTCTGTTCCTTCAAAACCTCCCTTTCTCCTCCTCCCATGGAAAATTCCTCCAAATCACCCGATGATCGGAAATCCCGCTTCTATCACCCTTACCAAGACCTACACGTTCCGATCACCAAGCTCTACGAGCTCCCCACCGCCCCCGAGCATCTTTTCTTCGAAGAAGCCGCCAGGCCTCACCGTTCTTGGGGCGAAAACCTCCAATATTACACCGGAATTGGCTACCTCTCCGGCGCCCTTTTTGGCGGCGCCAGGGGTTCCATTCAGGGCCTCAGGGCAGCTGAGCCTGGTGACTCTGTCAAACTCCGTCTCAATCGAGTTCTCAACTCTGGGGGCCAGCTTGGCCGGAGGGCTGGAAACTCTCTTGGGATCCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTATTCATTTGAGAGGTTCTGATGATGTTTTAAACAGTATTGTAGCTGGATTGGGGACTGGTGCTGTTTATAAGGCGGCGTCTGGGCCAAGGTCGGCTGCCATCGCCGGTGCTATTGGAGGAATTGCTGCTGCAGCTGCAGTTGCGGGTAAGCAGGCAGTTAAGAGATATGTGCCGATATAGAATTGGCATAACAAGTTATGCAAATTTGTACTAACTAGAGGGAGGATTTGCACGGTAAAGCATTTGGTTTCTTTGATTATTATTTCTTCATATTGTTATACAGTTAGTTTATTATTCATGGATTAGTGAATAATACACTTTTGGGTTGCAAATGCTCGCTCTTGTTATTTGTAATTGTTATTCATTTTGTTGATTTGCCTCTTATAGTTATTGATGCCCTGGATCAATGGGAGACTTTAAATTGGTGTTTGATTAATCTCTTCTTATGTGTAACTGACTCTGAAGCCTTCGTAATTATCTTGCCTGTGTTGGATCTCATTACAATGTAGCCATAGTACAGTATTGGAAAAAAGACTTTGCATAGATGACTGATTTCTTTATAATTTGTGGTTTATTGGAAAGTAGGGGATGGGTTTGGTGGTGACTTATTGTTGGGAGGGTTCATCTGAATCAATGAATAATACCAACGGAGTTTGTGGTAGCAGCCATTATTTTCTGTGTTATAGGCTAAAAGTGCAGGGGTTTCTTAGTTATATTGGCGAAGTTTCCGGAGGCCTAAATTTTGTCAAATAAGCCATTTTTCTTTACCAATACAATAGTCTTGGAAGTCTTGTCAAATATGGCGCTTAGAGTAGTTTTCCAAAGGTAGATATTGGTGGGGGGAAT

Coding sequence (CDS)

ATGGAAAATTCCTCCAAATCACCCGATGATCGGAAATCCCGCTTCTATCACCCTTACCAAGACCTACACGTTCCGATCACCAAGCTCTACGAGCTCCCCACCGCCCCCGAGCATCTTTTCTTCGAAGAAGCCGCCAGGCCTCACCGTTCTTGGGGCGAAAACCTCCAATATTACACCGGAATTGGCTACCTCTCCGGCGCCCTTTTTGGCGGCGCCAGGGGTTCCATTCAGGGCCTCAGGGCAGCTGAGCCTGGTGACTCTGTCAAACTCCGTCTCAATCGAGTTCTCAACTCTGGGGGCCAGCTTGGCCGGAGGGCTGGAAACTCTCTTGGGATCCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTATTCATTTGAGAGGTTCTGATGATGTTTTAAACAGTATTGTAGCTGGATTGGGGACTGGTGCTGTTTATAAGGCGGCGTCTGGGCCAAGGTCGGCTGCCATCGCCGGTGCTATTGGAGGAATTGCTGCTGCAGCTGCAGTTGCGGGTAAGCAGGCAGTTAAGAGATATGTGCCGATATAG

Protein sequence

MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGLESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRYVPI*
Homology
BLAST of CSPI01G33690 vs. ExPASy Swiss-Prot
Match: Q38820 (Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis thaliana OX=3702 GN=TIM23-2 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.5e-52
Identity = 107/184 (58.15%), Postives = 135/184 (73.37%), Query Frame = 0

Query: 3   NSSKSPDDRKSRFYHPYQDLHVPITK---LYELPTAPEHLFFEEAARPHRSWGENLQYYT 62
           N S    D  +R Y+PYQ+  VPI K   LY+LPT+PE LF EEA R  RSWGENL +YT
Sbjct: 5   NRSDHGSDENTRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENLTFYT 64

Query: 63  GIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 122
           G  YL G++ G + G I G+++ E GD+ KL++NR+LNS GQ GR  GN +GI+GL++AG
Sbjct: 65  GTAYLGGSVAGASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGLVYAG 124

Query: 123 LESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 182
           +ESG++     DDV  S+VAGLGTGAV +AA G RSAA+AGA+GG+AA A VAGKQ VKR
Sbjct: 125 IESGIVAATDRDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQIVKR 184

Query: 183 YVPI 184
           YVPI
Sbjct: 185 YVPI 188

BLAST of CSPI01G33690 vs. ExPASy Swiss-Prot
Match: Q9S837 (Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis thaliana OX=3702 GN=TIM23-3 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.0e-51
Identity = 102/184 (55.43%), Postives = 141/184 (76.63%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           M +S+     +K R Y+PYQ +++P  KLYELPT+PE LF EEA +   +WGENL ++TG
Sbjct: 5   MNHSTGHQQQQKYRQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTG 64

Query: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120
            GY +G++ G  +G+I G+RAAE G+S+K+R NR+LNSGG + RR GN LG +GL+FA +
Sbjct: 65  WGYCTGSVLGAFKGTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAM 124

Query: 121 ESGVIHLR-GSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180
           ESGV ++R G D  L +++AGL TG +Y+AASGPRSA +AGA+GG+AA AAVAG++ VKR
Sbjct: 125 ESGVTYMRDGDDGSLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKR 184

Query: 181 YVPI 184
           +VPI
Sbjct: 185 FVPI 188

BLAST of CSPI01G33690 vs. ExPASy Swiss-Prot
Match: Q9LNQ1 (Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis thaliana OX=3702 GN=TIM23-1 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.7e-51
Identity = 105/185 (56.76%), Postives = 134/185 (72.43%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITK--LYELPTAPEHLFFEEAARPHRSWGENLQYY 60
           +  SS    D  +R YHPYQ+  VPI    LY+LPT+PE LF EE+ +  RSWGENL +Y
Sbjct: 3   INRSSDHESDENTRLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLTFY 62

Query: 61  TGIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFA 120
           TG GYL+G++ G + G   G+++ E GD+ KL++NR+LNS GQ GR  GN +GI+GLI+A
Sbjct: 63  TGTGYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLIYA 122

Query: 121 GLESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVK 180
           G+ESGV+ +   DDV  S+VAGLGTGAV++AA G RSAA+AGA GGIAA A VAGKQ  K
Sbjct: 123 GIESGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQVFK 182

Query: 181 RYVPI 184
           RY  I
Sbjct: 183 RYAHI 187

BLAST of CSPI01G33690 vs. ExPASy Swiss-Prot
Match: Q9USM7 (Mitochondrial import inner membrane translocase subunit tim23 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tim23 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.9e-16
Identity = 58/160 (36.25%), Postives = 84/160 (52.50%), Query Frame = 0

Query: 17  HPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFGGARGSI 76
           HP  DL  P+  L     A   L  +  A P R W ++L Y TG  YLSG   GG  G  
Sbjct: 45  HPLADLDKPLDYLLIEEDALSTLPGDSMAIPSRGWQDDLCYGTGTSYLSGLAIGGLWGLN 104

Query: 77  QGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGLESGVIHLRGSDDVLNS 136
           +G++  +   S +LRLN +LN   + G   GNSLG+L L++ G+ S + + R      NS
Sbjct: 105 EGMKKTKDITSTRLRLNGILNGVTRRGPFVGNSLGVLALVYNGINSLIGYKRQKHGWENS 164

Query: 137 IVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQA 177
           + AG  TGA+YK+  G R+ AI+ ++   AA      K++
Sbjct: 165 VAAGALTGALYKSTRGLRAMAISSSLVATAAGIWTLAKRS 204

BLAST of CSPI01G33690 vs. ExPASy Swiss-Prot
Match: P32897 (Mitochondrial import inner membrane translocase subunit TIM23 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIM23 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.6e-12
Identity = 51/125 (40.80%), Postives = 69/125 (55.20%), Query Frame = 0

Query: 47  PHRSWGENLQYYTGIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRA 106
           P R W ++L Y TG  YL G   GG  G +QGL+   P    KL+LN VLN   + G   
Sbjct: 89  PSRGWTDDLCYGTGAVYLLGLGIGGFSGMMQGLQNIPPNSPGKLQLNTVLNHITKRGPFL 148

Query: 107 GNSLGILGLIFAGLESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIA 166
           GN+ GIL L +  + S +  LRG  D   SI AG  TGA++K++ G +    + A+  +A
Sbjct: 149 GNNAGILALSYNIINSTIDALRGKHDTAGSIGAGALTGALFKSSKGLKPMGYSSAM--VA 208

Query: 167 AAAAV 172
           AA AV
Sbjct: 209 AACAV 211

BLAST of CSPI01G33690 vs. ExPASy TrEMBL
Match: A0A0A0M0I6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690355 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 3.8e-94
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120
           IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180
           ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 184
           VPI
Sbjct: 181 VPI 183

BLAST of CSPI01G33690 vs. ExPASy TrEMBL
Match: A0A5A7V5P8 (Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G001650 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 4.2e-93
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MENSSKSPDDRK+RFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120
           IGYLSGAL GGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180
           ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 184
           VPI
Sbjct: 181 VPI 183

BLAST of CSPI01G33690 vs. ExPASy TrEMBL
Match: A0A1S3CJ69 (mitochondrial import inner membrane translocase subunit TIM23-2 OS=Cucumis melo OX=3656 GN=LOC103501018 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 4.2e-93
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MENSSKSPDDRK+RFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120
           IGYLSGAL GGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180
           ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 184
           VPI
Sbjct: 181 VPI 183

BLAST of CSPI01G33690 vs. ExPASy TrEMBL
Match: A0A6J1JGM0 (mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbita maxima OX=3661 GN=LOC111485524 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 2.2e-89
Identity = 177/184 (96.20%), Postives = 181/184 (98.37%), Query Frame = 0

Query: 1   MENSSKSPDDRK-SRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT 60
           ME+SSKS DDRK +RFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT
Sbjct: 1   MEDSSKSGDDRKNTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT 60

Query: 61  GIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 120
           GIGYLSGAL GGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG
Sbjct: 61  GIGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 120

Query: 121 LESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180
           LESGVIHLRG+DDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR
Sbjct: 121 LESGVIHLRGTDDVLNSIVAGLGTGALYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180

Query: 181 YVPI 184
           YVPI
Sbjct: 181 YVPI 184

BLAST of CSPI01G33690 vs. ExPASy TrEMBL
Match: A0A6J1H9R2 (mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbita moschata OX=3662 GN=LOC111461819 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 8.2e-89
Identity = 176/184 (95.65%), Postives = 180/184 (97.83%), Query Frame = 0

Query: 1   MENSSKSPDDRK-SRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT 60
           ME+SS S DDRK +RFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT
Sbjct: 1   MEDSSNSGDDRKNTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT 60

Query: 61  GIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 120
           GIGYLSGAL GGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG
Sbjct: 61  GIGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 120

Query: 121 LESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180
           LESGVIHLRG+DDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR
Sbjct: 121 LESGVIHLRGTDDVLNSIVAGLGTGALYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180

Query: 181 YVPI 184
           YVPI
Sbjct: 181 YVPI 184

BLAST of CSPI01G33690 vs. NCBI nr
Match: XP_008462719.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM23-2 [Cucumis melo] >KAA0062574.1 mitochondrial import inner membrane translocase subunit TIM23-2 [Cucumis melo var. makuwa] >TYK28799.1 mitochondrial import inner membrane translocase subunit TIM23-2 [Cucumis melo var. makuwa])

HSP 1 Score: 350.5 bits (898), Expect = 8.7e-93
Identity = 181/183 (98.91%), Postives = 182/183 (99.45%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MENSSKSPDDRK+RFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120
           IGYLSGAL GGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180
           ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 184
           VPI
Sbjct: 181 VPI 183

BLAST of CSPI01G33690 vs. NCBI nr
Match: XP_004142525.2 (LOW QUALITY PROTEIN: mitochondrial import inner membrane translocase subunit TIM23-3 [Cucumis sativus] >XP_031736235.1 LOW QUALITY PROTEIN: mitochondrial import inner membrane translocase subunit TIM23-3 [Cucumis sativus])

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 181/183 (98.91%), Postives = 181/183 (98.91%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120
           IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLG  GLIFAGL
Sbjct: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGSXGLIFAGL 120

Query: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180
           ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY
Sbjct: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 184
           VPI
Sbjct: 181 VPI 183

BLAST of CSPI01G33690 vs. NCBI nr
Match: XP_022988221.1 (mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita maxima])

HSP 1 Score: 338.2 bits (866), Expect = 4.5e-89
Identity = 177/184 (96.20%), Postives = 181/184 (98.37%), Query Frame = 0

Query: 1   MENSSKSPDDRK-SRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT 60
           ME+SSKS DDRK +RFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT
Sbjct: 1   MEDSSKSGDDRKNTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT 60

Query: 61  GIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 120
           GIGYLSGAL GGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG
Sbjct: 61  GIGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 120

Query: 121 LESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180
           LESGVIHLRG+DDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR
Sbjct: 121 LESGVIHLRGTDDVLNSIVAGLGTGALYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180

Query: 181 YVPI 184
           YVPI
Sbjct: 181 YVPI 184

BLAST of CSPI01G33690 vs. NCBI nr
Match: XP_038878582.1 (mitochondrial import inner membrane translocase subunit TIM23-2-like [Benincasa hispida])

HSP 1 Score: 337.4 bits (864), Expect = 7.6e-89
Identity = 174/183 (95.08%), Postives = 178/183 (97.27%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           ME+SSKS  DRK+RFYHPYQDLHVPI KLYELPTAPEHLFFEEAARPHRSWGENLQYYTG
Sbjct: 1   MEDSSKSAHDRKTRFYHPYQDLHVPIAKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60

Query: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120
           IGYLSGAL GGARGS+QGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL
Sbjct: 61  IGYLSGALLGGARGSVQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120

Query: 121 ESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180
           ESGVIHLRG DDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY
Sbjct: 121 ESGVIHLRGRDDVLNSIVAGLGTGALYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKRY 180

Query: 181 VPI 184
           VPI
Sbjct: 181 VPI 183

BLAST of CSPI01G33690 vs. NCBI nr
Match: XP_022961272.1 (mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita moschata] >XP_023534786.1 mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita pepo subsp. pepo] >KAG6590277.1 Mitochondrial import inner membrane translocase subunit TIM23-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033438.1 Mitochondrial import inner membrane translocase subunit TIM23-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 336.3 bits (861), Expect = 1.7e-88
Identity = 176/184 (95.65%), Postives = 180/184 (97.83%), Query Frame = 0

Query: 1   MENSSKSPDDRK-SRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT 60
           ME+SS S DDRK +RFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT
Sbjct: 1   MEDSSNSGDDRKNTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYT 60

Query: 61  GIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 120
           GIGYLSGAL GGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG
Sbjct: 61  GIGYLSGALLGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 120

Query: 121 LESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180
           LESGVIHLRG+DDVLNSIVAGLGTGA+YKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR
Sbjct: 121 LESGVIHLRGTDDVLNSIVAGLGTGALYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180

Query: 181 YVPI 184
           YVPI
Sbjct: 181 YVPI 184

BLAST of CSPI01G33690 vs. TAIR 10
Match: AT1G72750.1 (translocase inner membrane subunit 23-2 )

HSP 1 Score: 206.1 bits (523), Expect = 2.5e-53
Identity = 107/184 (58.15%), Postives = 135/184 (73.37%), Query Frame = 0

Query: 3   NSSKSPDDRKSRFYHPYQDLHVPITK---LYELPTAPEHLFFEEAARPHRSWGENLQYYT 62
           N S    D  +R Y+PYQ+  VPI K   LY+LPT+PE LF EEA R  RSWGENL +YT
Sbjct: 5   NRSDHGSDENTRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENLTFYT 64

Query: 63  GIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAG 122
           G  YL G++ G + G I G+++ E GD+ KL++NR+LNS GQ GR  GN +GI+GL++AG
Sbjct: 65  GTAYLGGSVAGASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGLVYAG 124

Query: 123 LESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 182
           +ESG++     DDV  S+VAGLGTGAV +AA G RSAA+AGA+GG+AA A VAGKQ VKR
Sbjct: 125 IESGIVAATDRDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQIVKR 184

Query: 183 YVPI 184
           YVPI
Sbjct: 185 YVPI 188

BLAST of CSPI01G33690 vs. TAIR 10
Match: AT3G04800.1 (translocase inner membrane subunit 23-3 )

HSP 1 Score: 204.5 bits (519), Expect = 7.2e-53
Identity = 102/184 (55.43%), Postives = 141/184 (76.63%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTG 60
           M +S+     +K R Y+PYQ +++P  KLYELPT+PE LF EEA +   +WGENL ++TG
Sbjct: 5   MNHSTGHQQQQKYRQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTG 64

Query: 61  IGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFAGL 120
            GY +G++ G  +G+I G+RAAE G+S+K+R NR+LNSGG + RR GN LG +GL+FA +
Sbjct: 65  WGYCTGSVLGAFKGTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAM 124

Query: 121 ESGVIHLR-GSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVKR 180
           ESGV ++R G D  L +++AGL TG +Y+AASGPRSA +AGA+GG+AA AAVAG++ VKR
Sbjct: 125 ESGVTYMRDGDDGSLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKR 184

Query: 181 YVPI 184
           +VPI
Sbjct: 185 FVPI 188

BLAST of CSPI01G33690 vs. TAIR 10
Match: AT1G17530.1 (translocase of inner mitochondrial membrane 23 )

HSP 1 Score: 203.8 bits (517), Expect = 1.2e-52
Identity = 105/185 (56.76%), Postives = 134/185 (72.43%), Query Frame = 0

Query: 1   MENSSKSPDDRKSRFYHPYQDLHVPITK--LYELPTAPEHLFFEEAARPHRSWGENLQYY 60
           +  SS    D  +R YHPYQ+  VPI    LY+LPT+PE LF EE+ +  RSWGENL +Y
Sbjct: 3   INRSSDHESDENTRLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLTFY 62

Query: 61  TGIGYLSGALFGGARGSIQGLRAAEPGDSVKLRLNRVLNSGGQLGRRAGNSLGILGLIFA 120
           TG GYL+G++ G + G   G+++ E GD+ KL++NR+LNS GQ GR  GN +GI+GLI+A
Sbjct: 63  TGTGYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLIYA 122

Query: 121 GLESGVIHLRGSDDVLNSIVAGLGTGAVYKAASGPRSAAIAGAIGGIAAAAAVAGKQAVK 180
           G+ESGV+ +   DDV  S+VAGLGTGAV++AA G RSAA+AGA GGIAA A VAGKQ  K
Sbjct: 123 GIESGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQVFK 182

Query: 181 RYVPI 184
           RY  I
Sbjct: 183 RYAHI 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q388203.5e-5258.15Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis t... [more]
Q9S8371.0e-5155.43Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis t... [more]
Q9LNQ11.7e-5156.76Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis t... [more]
Q9USM76.9e-1636.25Mitochondrial import inner membrane translocase subunit tim23 OS=Schizosaccharom... [more]
P328974.6e-1240.80Mitochondrial import inner membrane translocase subunit TIM23 OS=Saccharomyces c... [more]
Match NameE-valueIdentityDescription
A0A0A0M0I63.8e-94100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690355 PE=4 SV=1[more]
A0A5A7V5P84.2e-9398.91Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Cucumis melo ... [more]
A0A1S3CJ694.2e-9398.91mitochondrial import inner membrane translocase subunit TIM23-2 OS=Cucumis melo ... [more]
A0A6J1JGM02.2e-8996.20mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbit... [more]
A0A6J1H9R28.2e-8995.65mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
XP_008462719.18.7e-9398.91PREDICTED: mitochondrial import inner membrane translocase subunit TIM23-2 [Cucu... [more]
XP_004142525.21.5e-9298.91LOW QUALITY PROTEIN: mitochondrial import inner membrane translocase subunit TIM... [more]
XP_022988221.14.5e-8996.20mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita ... [more]
XP_038878582.17.6e-8995.08mitochondrial import inner membrane translocase subunit TIM23-2-like [Benincasa ... [more]
XP_022961272.11.7e-8895.65mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT1G72750.12.5e-5358.15translocase inner membrane subunit 23-2 [more]
AT3G04800.17.2e-5355.43translocase inner membrane subunit 23-3 [more]
AT1G17530.11.2e-5256.76translocase of inner mitochondrial membrane 23 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF02466Tim17coord: 57..165
e-value: 7.5E-23
score: 81.1
NoneNo IPR availablePANTHERPTHR15371TIM23coord: 1..183
NoneNo IPR availablePANTHERPTHR15371:SF24MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM23-3coord: 1..183

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G33690.1CSPI01G33690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0031305 integral component of mitochondrial inner membrane
cellular_component GO:0005744 TIM23 mitochondrial import inner membrane translocase complex
molecular_function GO:0008320 protein transmembrane transporter activity