CSPI01G33470 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G33470
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProtein kinase domain-containing protein
LocationChr1: 28413894 .. 28415974 (-)
RNA-Seq ExpressionCSPI01G33470
SyntenyCSPI01G33470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAAGTAAAAAGGATACCAAAAGAAAGAATATTCTAAATAAGGATCCAATATCCTCTAAGCCTTCTATAAAAATCCCCCAATTCCCAACTGATTCCCCCAAAAGCACTCCTACTTCCTAAGACTAAAGAGAAAGAGAAAGAGAAAGAGAGAAAGAGAGAAACAAGTTCAAGAATTTTGTAGAGAGCAAATACCAAAATCCCCCAATTTTTCCAAAGAAGAAGAACAAACAAATCTTCAGCAATGGCCGACATGATCGTCAACAGCCAATACCAAATCCTCGAGGAGCTCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGCTTGATTTCCGACCTCTGCGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCCGAAGCTGCCGTCGTCATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTGTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTCTCAGCCGAACAAGCTCTCAGTAAGTCTAATTCTTATATTCAATCAAACCATTTTATGTTCTTGATCTGGTTGTTCTGGCAACTGTTTATTGATTGATTCCATATTTTTTTAGCAAATGGTTGAACCATTTGAAGAAACCCCAAAACCCCAAGTGAAAATTCATTGCATATTTCAAAACTAGTGAATCTATTTATTTTCTTATGGGAGTTGGAAACTGTTGTTTTGCAGGACACCCATGGATATTGAGTGGAGGAGAAGCAACATCTTCAGTTGAATAATTTGATCTTAACACTTTTTAACTCATCCCTAAAAAGAGAAACATATGAAGAAAGGATGCTATTGAAAAAGAATCAAGCATTCCTGTTTTTGTATTCAAGTACATAATCCCCTGCTCTGCTTCTTCACCATATTGTGAATAAGAGACCTTAACCTTCAGTTCAGACTCCATCAATGGCTGCCTGCTCCATGTATAAGGTTCTAGAGAGAGAGAAAGATAACATCAGTTGTTGATTTGAGACTCCGCTGCTGTCAATATCATCTTTCTTTTCTTTTTTGATTTCTTCAATGCTTTGAGAAGCATTTGAGGATGTTTTTTTTTCTTTTTTTGCCTTTTTCATTTTGATTTGTTGTTAGGTAAAAACGGAATTTGGGGAATCCTTTCTTTCCCTCCCAACCCTTTTCTTTTTGTTGTATAAAGTGTTTGGGAAGTGAGAAAGTCCCACAAAAAAAATGGGAAAAGAATTAGAAAAAGGAGAGAGAAAAATAGTGAGAGAGAGATAAAGAAGAGAAGTTGTATTCAAATTAGTAGATGGCCTTTTTGTTTTGTTTCTTTTTTTTCAATTTTCAAGGATGTAATGAAATGTGATGTGATGTGGATGCAATTTGCTGGGAATATAAGGGTGATGTTTTAGTGTAAGTTTGCTTAACTTCAACTTCTTTTGTTTGACTATTTTGTTTTGGGTTTAAATGCACATCAGATTCTAAGGCATAGAGTTGGTATATGCTTTTCTCATATATATATACCCATTTCGGTTAAAAAGAAATACCTATGTTGATCAATTTTGATTGATATATTT

mRNA sequence

CCAAAAGTAAAAAGGATACCAAAAGAAAGAATATTCTAAATAAGGATCCAATATCCTCTAAGCCTTCTATAAAAATCCCCCAATTCCCAACTGATTCCCCCAAAAGCACTCCTACTTCCTAAGACTAAAGAGAAAGAGAAAGAGAAAGAGAGAAAGAGAGAAACAAGTTCAAGAATTTTGTAGAGAGCAAATACCAAAATCCCCCAATTTTTCCAAAGAAGAAGAACAAACAAATCTTCAGCAATGGCCGACATGATCGTCAACAGCCAATACCAAATCCTCGAGGAGCTCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGCTTGATTTCCGACCTCTGCGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCCGAAGCTGCCGTCGTCATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTGTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTCTCAGCCGAACAAGCTCTCAGACACCCATGGATATTGAGTGGAGGAGAAGCAACATCTTCAGTTGAATAATTTGATCTTAACACTTTTTAACTCATCCCTAAAAAGAGAAACATATGAAGAAAGGATGCTATTGAAAAAGAATCAAGCATTCCTGTTTTTGTATTCAAGTACATAATCCCCTGCTCTGCTTCTTCACCATATTGTGAATAAGAGACCTTAACCTTCAGTTCAGACTCCATCAATGGCTGCCTGCTCCATGTATAAGGTTCTAGAGAGAGAGAAAGATAACATCAGTTGTTGATTTGAGACTCCGCTGCTGTCAATATCATCTTTCTTTTCTTTTTTGATTTCTTCAATGCTTTGAGAAGCATTTGAGGATGTTTTTTTTTCTTTTTTTGCCTTTTTCATTTTGATTTGTTGTTAGGTAAAAACGGAATTTGGGGAATCCTTTCTTTCCCTCCCAACCCTTTTCTTTTTGTTGTATAAAGTGTTTGGGAAGTGAGAAAGTCCCACAAAAAAAATGGGAAAAGAATTAGAAAAAGGAGAGAGAAAAATAGTGAGAGAGAGATAAAGAAGAGAAGTTGTATTCAAATTAGTAGATGGCCTTTTTGTTTTGTTTCTTTTTTTTCAATTTTCAAGGATGTAATGAAATGTGATGTGATGTGGATGCAATTTGCTGGGAATATAAGGGTGATGTTTTAGTGTAAGTTTGCTTAACTTCAACTTCTTTTGTTTGACTATTTTGTTTTGGGTTTAAATGCACATCAGATTCTAAGGCATAGAGTTGGTATATGCTTTTCTCATATATATATACCCATTTCGGTTAAAAAGAAATACCTATGTTGATCAATTTTGATTGATATATTT

Coding sequence (CDS)

ATGGCCGACATGATCGTCAACAGCCAATACCAAATCCTCGAGGAGCTCGGAAGAGGAAGGTTCGGAATCATCACTCGTTGCTTTTGTCCAATCTCCAACAAGTTCTTCGCCTGCAAAACCATCTCTAAAAACGATCTCAACGATGAAACTGACAAAGAATGCCTTGTCAAAGAACCCAAAATCATGGCTCTTCTCCCTCCCCATCCCAACATTCTTCAGTTAATCGACGTTCTCGAGAATGACCATTACCTCTGCTTGATTTCCGACCTCTGCGAATCCCTCTCCCTTTACGATCGGATCATCCGTCGTCCCTTTTCCGAATCCGAAGCTGCCGTCGTCATGAAGCAGCTTCTCCAAGCCCTCGCCCACTGTCATTCTCATGGCGTCGTTCATCGCGATGTCAAGCCTGACAATGTGCTCTTCGATTCCAGGAACAATTTGAAACTCATCGATTTCGGATCGGCTGAGTGGTGTGATGAAGATGGATTTATGTACGGTGTCGTCGGAACGCCTTATTACGTTGCGCCGGAAGTTTTGAGAGGATCTGAGTACGGTCGGAAGGTCGACGTGTGGAGCGCCGGTGTGATTCTTTACACGATGTTGGCCGGATTTCCCCCGTTCTACGGTGAATCTGCGGCGGAGGTCTTTGAGGCTGTTCTTAGAGGAAATCTCAGATTTCCGACTAGGGTTTTTAGGTCGGTTTCTTCAGCGGCTAAGGATCTGATGAAGAAGATGATCTGTCGAGATGTTTCTAGAAGATTCTCAGCCGAACAAGCTCTCAGACACCCATGGATATTGAGTGGAGGAGAAGCAACATCTTCAGTTGAATAA

Protein sequence

MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQALAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE*
Homology
BLAST of CSPI01G33470 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.0e-92
Identity = 161/266 (60.53%), Postives = 203/266 (76.32%), Query Frame = 0

Query: 8   SQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPP 67
           ++YQI EE+GRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHPWILSGGE 271
           +IC+D SRRFSAEQALRHPWI   GE
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of CSPI01G33470 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 323.6 bits (828), Expect = 2.3e-87
Identity = 157/273 (57.51%), Postives = 203/273 (74.36%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           + + YQ+ +E+GRGRFG ITRCF P + +F+ACKTI K  L D  D+EC+  EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR--PFSESEAAVVMKQLLQALA 125
           PPHPNI+++ D+ E +  L ++ +L +  +++YDR+I      SESE+A   KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHSHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +Y  KVD+WSAGV++YTMLAG PPF GE+A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATSS 275
           ++KMICRDVSRRFSAE ALRH W+++ G   S+
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVGNLQSN 278

BLAST of CSPI01G33470 vs. ExPASy Swiss-Prot
Match: P93759 (Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 6.4e-58
Identity = 116/272 (42.65%), Postives = 173/272 (63.60%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y++  ELGRG FG+   C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L +  E+D  + L+ +LCE   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATS 274
           +KKM+  D  RR +A+Q L HPWI +G  A++
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNGKNASN 320

BLAST of CSPI01G33470 vs. ExPASy Swiss-Prot
Match: P28582 (Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2)

HSP 1 Score: 225.7 bits (574), Expect = 6.4e-58
Identity = 113/271 (41.70%), Postives = 176/271 (64.94%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y + +ELGRG+FG + +C    S + +ACK+I K  L  + DKE + +E +I+  L
Sbjct: 77  IRGKYTLGKELGRGQFGCVYQCTENSSGQLYACKSILKRKLVSKNDKEDIKREIQILQHL 136

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
              PNI++   V E+   + L+ +LC    L+DRII +  +SE  AA + +Q++  +  C
Sbjct: 137 SGQPNIVEFKGVFEDRQSVHLVMELCAGGELFDRIIAQGHYSERAAATICRQIVNVVHVC 196

Query: 126 HSHGVVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H  GV+HRD+KP+N L  S++    LK  DFG + + +E      +VG+ YYVAPEVLR 
Sbjct: 197 HFMGVMHRDLKPENFLLSSKDKDAMLKATDFGLSVFIEEGKVYRNIVGSAYYVAPEVLRR 256

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L+G PPF+ E+   +F+A+L G + F +  + SVS++AKDL
Sbjct: 257 S-YGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDAILEGVIDFESEPWPSVSNSAKDL 316

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEAT 273
           ++KM+ +D  RR ++ Q L HPW+  GGEA+
Sbjct: 317 VRKMLTQDPRRRITSAQVLDHPWMREGGEAS 346

BLAST of CSPI01G33470 vs. ExPASy Swiss-Prot
Match: Q9C6P3 (Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.4e-57
Identity = 110/267 (41.20%), Postives = 175/267 (65.54%), Query Frame = 0

Query: 10  YQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPPHP 69
           Y + +ELGRG+FG+   C    + K FACK+ISK  L  + DKE + +E +IM  L   P
Sbjct: 73  YTLSKELGRGQFGVTYLCTEKSTGKRFACKSISKKKLVTKGDKEDMRREIQIMQHLSGQP 132

Query: 70  NILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCHSHG 129
           NI++     E++  + L+ +LC    L+DRI+ +  +SE  AA V +Q++  +  CH  G
Sbjct: 133 NIVEFKGAYEDEKAVNLVMELCAGGELFDRILAKGHYSERAAASVCRQIVNVVNICHFMG 192

Query: 130 VVHRDVKPDNVLFDSRNN---LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEYG 189
           V+HRD+KP+N L  S++    +K  DFG + + +E      +VG+ YYVAPEVL+   YG
Sbjct: 193 VMHRDLKPENFLLSSKDEKALIKATDFGLSVFIEEGRVYKDIVGSAYYVAPEVLK-RRYG 252

Query: 190 RKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKKM 249
           +++D+WSAG+ILY +L+G PPF+ E+   +F+A+L G + F ++ + S+S++AKDL+++M
Sbjct: 253 KEIDIWSAGIILYILLSGVPPFWAETEKGIFDAILEGEIDFESQPWPSISNSAKDLVRRM 312

Query: 250 ICRDVSRRFSAEQALRHPWILSGGEAT 273
           + +D  RR SA + L+HPW+  GGEA+
Sbjct: 313 LTQDPKRRISAAEVLKHPWLREGGEAS 338

BLAST of CSPI01G33470 vs. ExPASy TrEMBL
Match: A0A0A0M3G7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690150 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.6e-157
Identity = 275/276 (99.64%), Postives = 275/276 (99.64%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of CSPI01G33470 vs. ExPASy TrEMBL
Match: A0A5A7TYV2 (Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00970 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.0e-154
Identity = 270/276 (97.83%), Postives = 271/276 (98.19%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEE+GRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRR SAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of CSPI01G33470 vs. ExPASy TrEMBL
Match: A0A1S3CHK9 (phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.0e-154
Identity = 270/276 (97.83%), Postives = 271/276 (98.19%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEE+GRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRR SAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of CSPI01G33470 vs. ExPASy TrEMBL
Match: A0A6J1JGI4 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485498 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 2.9e-146
Identity = 251/276 (90.94%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRRFSAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of CSPI01G33470 vs. ExPASy TrEMBL
Match: A0A6J1HBL3 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461799 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.9e-145
Identity = 249/276 (90.22%), Postives = 265/276 (96.01%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRRFSAEQALRHPWI+SGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 276

BLAST of CSPI01G33470 vs. NCBI nr
Match: XP_011660276.2 (LOW QUALITY PROTEIN: phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus])

HSP 1 Score: 562.4 bits (1448), Expect = 2.2e-156
Identity = 274/276 (99.28%), Postives = 274/276 (99.28%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEEL RGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEELXRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLP HPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPSHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 276

BLAST of CSPI01G33470 vs. NCBI nr
Match: XP_008462700.1 (PREDICTED: phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo] >KAA0046511.1 phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo var. makuwa] >TYK28749.1 phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 555.8 bits (1431), Expect = 2.1e-154
Identity = 270/276 (97.83%), Postives = 271/276 (98.19%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           MADMIVNSQYQILEE+GRGRFGIITRCFCPISNKFFACKTISK DL DETDKECLVKEPK
Sbjct: 1   MADMIVNSQYQILEEIGRGRFGIITRCFCPISNKFFACKTISKTDLIDETDKECLVKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDVLENDHYLCLISDLCE LSLYDRIIRRPFSESEAAVVMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCEPLSLYDRIIRRPFSESEAAVVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRR SAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRLSAEQALRHPWILSGGEGTSSVE 276

BLAST of CSPI01G33470 vs. NCBI nr
Match: XP_038877083.1 (phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida])

HSP 1 Score: 537.0 bits (1382), Expect = 9.9e-149
Identity = 255/274 (93.07%), Postives = 268/274 (97.81%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQYQILEE+GRGRFG ITRCFCPISNKFFACKTI+KNDL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYQILEEIGRGRFGTITRCFCPISNKFFACKTIAKNDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMA+LPPHPNILQLIDVLEND YLCLISDLC+SLSLYDRIIRRPFSESEAA+V+KQLL+A
Sbjct: 61  IMAILPPHPNILQLIDVLENDDYLCLISDLCDSLSLYDRIIRRPFSESEAAIVIKQLLEA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSA+WCDEDGFM+GVVGTPYY+APEVLR
Sbjct: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSADWCDEDGFMFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD
Sbjct: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSS 275
           LMKKMICRDVSRRFSAEQ+LRHPWILSGGE T++
Sbjct: 241 LMKKMICRDVSRRFSAEQSLRHPWILSGGEGTAT 274

BLAST of CSPI01G33470 vs. NCBI nr
Match: XP_022988181.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima])

HSP 1 Score: 527.7 bits (1358), Expect = 6.0e-146
Identity = 251/276 (90.94%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 1   MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 60

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 61  IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 120

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 121 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 180

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+SSAAKD
Sbjct: 181 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISSAAKD 240

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRRFSAEQALRHPWILSGGE TSSVE
Sbjct: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEGTSSVE 276

BLAST of CSPI01G33470 vs. NCBI nr
Match: KAG7023891.1 (Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 525.0 bits (1351), Expect = 3.9e-145
Identity = 249/276 (90.22%), Postives = 265/276 (96.01%), Query Frame = 0

Query: 1   MADMIVNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPK 60
           M DMIVNSQY+ILEE+GRGRFG ITRCFCPISNKFFACKTI+K+DL DETDKECL KEPK
Sbjct: 43  MGDMIVNSQYEILEEIGRGRFGTITRCFCPISNKFFACKTIAKSDLIDETDKECLEKEPK 102

Query: 61  IMALLPPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRRPFSESEAAVVMKQLLQA 120
           IMALLPPHPNILQLIDV END YLCLIS+LC+S+SLYDRIIRRPFSESEAA+VMKQLLQA
Sbjct: 103 IMALLPPHPNILQLIDVFENDDYLCLISELCDSVSLYDRIIRRPFSESEAAIVMKQLLQA 162

Query: 121 LAHCHSHGVVHRDVKPDNVLFDSRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLR 180
           LAHCH HGVVHRDVKPDNVLFDSR+NLKLIDFGSAEWCD+DGF +GVVGTPYY+APEVLR
Sbjct: 163 LAHCHYHGVVHRDVKPDNVLFDSRDNLKLIDFGSAEWCDKDGFTFGVVGTPYYIAPEVLR 222

Query: 181 GSEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKD 240
           GSEYG+KVDVWSAGVILYTMLAGFPPFYG+SAAEVFEAVLRGNLRFP RVFRS+S AAKD
Sbjct: 223 GSEYGQKVDVWSAGVILYTMLAGFPPFYGDSAAEVFEAVLRGNLRFPPRVFRSISPAAKD 282

Query: 241 LMKKMICRDVSRRFSAEQALRHPWILSGGEATSSVE 277
           LMKKMICRDVSRRFSAEQALRHPWI+SGGE TSSVE
Sbjct: 283 LMKKMICRDVSRRFSAEQALRHPWIMSGGEGTSSVE 318

BLAST of CSPI01G33470 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 339.7 bits (870), Expect = 2.2e-93
Identity = 161/266 (60.53%), Postives = 203/266 (76.32%), Query Frame = 0

Query: 8   SQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPP 67
           ++YQI EE+GRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQALRHPWILSGGE 271
           +IC+D SRRFSAEQALRHPWI   GE
Sbjct: 253 LICKDASRRFSAEQALRHPWIQRAGE 278

BLAST of CSPI01G33470 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 323.6 bits (828), Expect = 1.6e-88
Identity = 157/273 (57.51%), Postives = 203/273 (74.36%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           + + YQ+ +E+GRGRFG ITRCF P + +F+ACKTI K  L D  D+EC+  EP+IMA+L
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLIDALDRECIETEPRIMAML 66

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR--PFSESEAAVVMKQLLQALA 125
           PPHPNI+++ D+ E +  L ++ +L +  +++YDR+I      SESE+A   KQ+L ALA
Sbjct: 67  PPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSALA 126

Query: 126 HCHSHGVVHRDVKPDNVLFD-SRNNLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           HCH   VVHRDVKPDNVL D     +KL DFGSA W   +    GVVGTPYYVAPEV+ G
Sbjct: 127 HCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWLGGE-TAEGVVGTPYYVAPEVVMG 186

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
            +Y  KVD+WSAGV++YTMLAG PPF GE+A ++FE++LRGNLRFP + F SVSS AKDL
Sbjct: 187 RKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAKDL 246

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATSS 275
           ++KMICRDVSRRFSAE ALRH W+++ G   S+
Sbjct: 247 LRKMICRDVSRRFSAEDALRHSWMMNVGNLQSN 278

BLAST of CSPI01G33470 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 322.4 bits (825), Expect = 3.6e-88
Identity = 154/256 (60.16%), Postives = 196/256 (76.56%), Query Frame = 0

Query: 8   SQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALLPP 67
           ++YQI EE+GRGRFG ++R + P +  FFACKTI K  L+D+ D+ CL  EPK+MALL  
Sbjct: 13  NKYQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSY 72

Query: 68  HPNILQLIDVLENDHYLCLISDLCE-SLSLYDRIIRR-PFSESEAAVVMKQLLQALAHCH 127
           HPNI+Q+ D+++ D  L +  +L   S+S+YDR++    F E + A   KQ+LQAL+HCH
Sbjct: 73  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 132

Query: 128 SHGVVHRDVKPDNVLFDSRNN-LKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRGSEY 187
            +GVVHRD+KP+N+L D RN+ +K+ DFGS  W  E     GVVGTPYYVAPEVL G  Y
Sbjct: 133 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEGETTEGVVGTPYYVAPEVLMGYSY 192

Query: 188 GRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDLMKK 247
           G KVD+WSAGV+LYTMLAG PPFYGE+A E+FEAVLRGNLRFPT++FR VSS AKD ++K
Sbjct: 193 GEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRK 252

Query: 248 MICRDVSRRFSAEQAL 261
           +IC+D SRRFSAEQAL
Sbjct: 253 LICKDASRRFSAEQAL 268

BLAST of CSPI01G33470 vs. TAIR 10
Match: AT2G41860.2 (calcium-dependent protein kinase 14 )

HSP 1 Score: 226.1 bits (575), Expect = 3.5e-59
Identity = 116/272 (42.65%), Postives = 173/272 (63.60%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y++  ELGRG FG+   C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L +  E+D  + L+ +LCE   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGDRFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATS 274
           +KKM+  D  RR +A+Q L HPWI +G  A++
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNGKNASN 320

BLAST of CSPI01G33470 vs. TAIR 10
Match: AT2G41860.1 (calcium-dependent protein kinase 14 )

HSP 1 Score: 225.7 bits (574), Expect = 4.5e-59
Identity = 116/272 (42.65%), Postives = 173/272 (63.60%), Query Frame = 0

Query: 6   VNSQYQILEELGRGRFGIITRCFCPISNKFFACKTISKNDLNDETDKECLVKEPKIMALL 65
           +  +Y++  ELGRG FG+   C    + + FACK+I K  L    D E + +E +IM  +
Sbjct: 50  IKQKYKLGRELGRGEFGVTYLCTEIETGEIFACKSILKKKLKTSIDIEDVKREVEIMRQM 109

Query: 66  PPHPNILQLIDVLENDHYLCLISDLCESLSLYDRIIRR-PFSESEAAVVMKQLLQALAHC 125
           P HPNI+ L +  E+D  + L+ +LCE   L+DRI+ R  ++E  AA V+K +++ +  C
Sbjct: 110 PEHPNIVTLKETYEDDKAVHLVMELCEGGELFDRIVARGHYTERAAASVIKTIIEVVQMC 169

Query: 126 HSHGVVHRDVKPDNVLFDSRN---NLKLIDFGSAEWCDEDGFMYGVVGTPYYVAPEVLRG 185
           H HGV+HRD+KP+N LF ++    +LK IDFG + +         +VG+PYY+APEVLR 
Sbjct: 170 HKHGVMHRDLKPENFLFANKKETASLKAIDFGLSVFFKPGERFNEIVGSPYYMAPEVLRR 229

Query: 186 SEYGRKVDVWSAGVILYTMLAGFPPFYGESAAEVFEAVLRGNLRFPTRVFRSVSSAAKDL 245
           S YG+++D+WSAGVILY +L G PPF+ E+   V +A+L+  + F    +  VS  AKDL
Sbjct: 230 S-YGQEIDIWSAGVILYILLCGVPPFWAETEHGVAKAILKSVIDFKRDPWPKVSDNAKDL 289

Query: 246 MKKMICRDVSRRFSAEQALRHPWILSGGEATS 274
           +KKM+  D  RR +A+Q L HPWI +G  A++
Sbjct: 290 IKKMLHPDPRRRLTAQQVLDHPWIQNGKNASN 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SPK43.0e-9260.53Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK02.3e-8757.51Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
P937596.4e-5842.65Calcium-dependent protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CPK14 PE=... [more]
P285826.4e-5841.70Calcium-dependent protein kinase OS=Daucus carota OX=4039 PE=2 SV=2[more]
Q9C6P31.4e-5741.20Calcium-dependent protein kinase 33 OS=Arabidopsis thaliana OX=3702 GN=CPK33 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0M3G71.6e-15799.64Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G690... [more]
A0A5A7TYV21.0e-15497.83Phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CHK91.0e-15497.83phosphoenolpyruvate carboxylase kinase 1 OS=Cucumis melo OX=3656 GN=LOC103501000... [more]
A0A6J1JGI42.9e-14690.94phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HBL31.9e-14590.22phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_011660276.22.2e-15699.28LOW QUALITY PROTEIN: phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus][more]
XP_008462700.12.1e-15497.83PREDICTED: phosphoenolpyruvate carboxylase kinase 1 [Cucumis melo] >KAA0046511.1... [more]
XP_038877083.19.9e-14993.07phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida][more]
XP_022988181.16.0e-14690.94phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita maxima][more]
KAG7023891.13.9e-14590.22Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
AT1G08650.12.2e-9360.53phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.11.6e-8857.51phosphoenolpyruvate carboxylase kinase 2 [more]
AT1G08650.23.6e-8860.16phosphoenolpyruvate carboxylase kinase 1 [more]
AT2G41860.23.5e-5942.65calcium-dependent protein kinase 14 [more]
AT2G41860.14.5e-5942.65calcium-dependent protein kinase 14 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 10..265
e-value: 1.6E-92
score: 323.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 10..265
e-value: 4.7E-72
score: 242.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 10..265
score: 47.933353
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 3..275
e-value: 6.7E-88
score: 297.1
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 6..274
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 6..274
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 9..264
e-value: 4.111E-124
score: 352.164
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 129..141
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 16..39
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 4..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G33470.1CSPI01G33470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity