CSPI01G33230 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G33230
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Defensin-like family protein, putative
Location	Chr1: 28110244 .. 28110627 (+)
RNA-Seq Expression	CSPI01G33230
Synteny	CSPI01G33230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAAGTTGGAGTTATTTCTTGCATTCTCATTGCCTTTCTCGTCTTGTATTCAGGTCGTCTGACTTTATTTATTTATCTTTTTAAATGACTTTAGTCTGAAATTATATGGTTGGATGAAAACCTAATTAAGTGCTTTCTTCTAAAGTAGATACTGTTAATTTGTTAACATTAAGTAATTGATGGAACTGCAGGAAGTTTGAAAACAAGAGCTCAGCTGTGTTGCAATGAACATCCTGAACTGGGGAGTTGCATACCTGGAGTAGACGACAAACATGATAATAGTGGTGAAGATGGAAAGTGTTGGAGTCATTGCATTTCTGGCTGTTTGAAAGGAGGATTTTGCAAGCCAAATAACCATATATGCCATTGTTATTGTTGA

mRNA sequence

ATGGCCAAAGTTGGAGTTATTTCTTGCATTCTCATTGCCTTTCTCGTCTTGTATTCAGGAAGTTTGAAAACAAGAGCTCAGCTGTGTTGCAATGAACATCCTGAACTGGGGAGTTGCATACCTGGAGTAGACGACAAACATGATAATAGTGGTGAAGATGGAAAGTGTTGGAGTCATTGCATTTCTGGCTGTTTGAAAGGAGGATTTTGCAAGCCAAATAACCATATATGCCATTGTTATTGTTGA

Coding sequence (CDS)

ATGGCCAAAGTTGGAGTTATTTCTTGCATTCTCATTGCCTTTCTCGTCTTGTATTCAGGAAGTTTGAAAACAAGAGCTCAGCTGTGTTGCAATGAACATCCTGAACTGGGGAGTTGCATACCTGGAGTAGACGACAAACATGATAATAGTGGTGAAGATGGAAAGTGTTGGAGTCATTGCATTTCTGGCTGTTTGAAAGGAGGATTTTGCAAGCCAAATAACCATATATGCCATTGTTATTGTTGA

Protein sequence

MAKVGVISCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSHCISGCLKGGFCKPNNHICHCYC*

Homology

[Close]

BLAST of CSPI01G33230 vs. ExPASy Swiss-Prot
Match: P0CAX9 (Defensin-like protein 21 OS=Arabidopsis thaliana OX=3702 GN=At4g14276 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 7.5e-15
Identity = 39/95 (41.05%), Postives = 53/95 (55.79%), Query Frame = 0


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDKHD 60
          M +  V+S +L A +VL  GS++   Q            +CC EHP +G C+P +D   D
Sbjct: 1  MVRTNVVSFVLFAAIVLCIGSIQIDGQKHTAPWIPEDNSICCKEHPSVGRCLPNID---D 60

Query: 61 NSGEDGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          ++ + GKCW  CI GC  GGFCK   + HICHC C
Sbjct: 61 SAEKGGKCWKFCIEGCETGGFCKLFGHKHICHCNC 92

[Close]

BLAST of CSPI01G33230 vs. ExPASy Swiss-Prot
Match: Q2V2S1 (Putative defensin-like protein 20 OS=Arabidopsis thaliana OX=3702 GN=At5g52605 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.7e-14
Identity = 38/91 (41.76%), Postives = 52/91 (57.14%), Query Frame = 0


Query: 1  MAKVGVISCILIAFLVL--------YSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGE 60
          M +  V+S +L A +VL         +  +     +CC EHP +G C+PG+D   D++ +
Sbjct: 1  MVRTNVVSFVLFAAIVLCILYGQKHIAPWIFEDKSICCKEHPSVGRCLPGID---DDAEK 60

Query: 61 DGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          DGKCW  CI GC  GGFCK   + HICHC C
Sbjct: 61 DGKCWKFCIEGCETGGFCKLFEHKHICHCNC 88

[Close]

BLAST of CSPI01G33230 vs. ExPASy Swiss-Prot
Match: Q2L6T3 (Putative defensin-like protein 27 OS=Arabidopsis thaliana OX=3702 GN=At4g18823 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 24/54 (44.44%), Postives = 28/54 (51.85%), Query Frame = 0


Query: 28 LCCNEHPELGSCIPGVDDKHDNSGEDGKCWSHCISGCLKGGFCKPNNHICHCYC 82
          LCCN+HPE G C             +  C   C+ GC +GGFCK    ICHCYC
Sbjct: 35 LCCNDHPEFGIC------------TNKSCNKWCLQGCTRGGFCK--RKICHCYC 74

[Close]

BLAST of CSPI01G33230 vs. ExPASy Swiss-Prot
Match: P0CAY0 (Putative defensin-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=At4g14272 PE=5 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.0e-04
Identity = 18/41 (43.90%), Postives = 23/41 (56.10%), Query Frame = 0


Query: 29 CCNEHPELGSCIPGVDDKHDNSGEDGKCWSHCISGCLKGGF 70
          C  EH E+G C+ G+DD   N   DGKCW  C+     GG+
Sbjct: 39 CNREHSEIGICLSGIDDDIQN---DGKCWKFCLMVAKSGGY 76

[Close]

BLAST of CSPI01G33230 vs. ExPASy TrEMBL
Match: A0A0A0LWJ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573030 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.0e-22
Identity = 53/84 (63.10%), Postives = 62/84 (73.81%), Query Frame = 0


Query: 1  MAKVGVI-SCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G I S + +AFLVLY+GS+ T AQLCCN H ELGSC+PGVDD +     DGKCW H
Sbjct: 1  MAKLGAIFSWVFVAFLVLYAGSMNTNAQLCCNNHRELGSCVPGVDDDY-----DGKCWHH 60

Query: 61 CISGCLKGGFCKP--NNHICHCYC 82
          CI GC +GGFCK     H+CHCYC
Sbjct: 61 CIVGCERGGFCKKLWYGHVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. ExPASy TrEMBL
Match: A0A5D3CFQ3 (Putative defensin-like protein 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003350 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.7e-22
Identity = 55/84 (65.48%), Postives = 61/84 (72.62%), Query Frame = 0


Query: 1  MAKVGVI-SCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G I   + IAFLVLYSGS  T AQLCCN HPELGSC PGVDD       DGKCW H
Sbjct: 1  MAKLGAIFGWVFIAFLVLYSGSKNTNAQLCCNNHPELGSCEPGVDD-----ASDGKCWLH 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI+GC KGGFCK   + ++CHCYC
Sbjct: 61 CIAGCEKGGFCKQLTDKNVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. ExPASy TrEMBL
Match: A0A0A0LZ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573070 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.5e-21
Identity = 50/85 (58.82%), Postives = 59/85 (69.41%), Query Frame = 0


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSHC 60
          MAKVG+ISC+L+ FLV YSG+L   A +CCN +P LGSC PGVD   D+   +GKCW  C
Sbjct: 1  MAKVGLISCVLVVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVD---DSPQANGKCWRLC 60

Query: 61 ISGCLKGGFCK----PNNHICHCYC 82
           SGC KGGFCK       H+CHCYC
Sbjct: 61 TSGCTKGGFCKRVGRNGRHVCHCYC 82

[Close]

BLAST of CSPI01G33230 vs. ExPASy TrEMBL
Match: A0A0A0M1N0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573060 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.3e-21
Identity = 52/84 (61.90%), Postives = 59/84 (70.24%), Query Frame = 0


Query: 1  MAKVG-VISCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G     + IAFLVLYSGS+ T AQLCCN HPELG C  GVDD       DGKCW H
Sbjct: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDD-----ANDGKCWLH 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI+GC KGGFCK   + ++CHCYC
Sbjct: 61 CIAGCEKGGFCKHLSDKNVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. ExPASy TrEMBL
Match: A0A0A0LYL5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573040 PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.3e-19
Identity = 51/84 (60.71%), Postives = 55/84 (65.48%), Query Frame = 0


Query: 1  MAKVGVIS-CILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAKVGVIS  + I FL  YSGSL T AQLCC  HPE G+C PGVDD       DG+CW  
Sbjct: 1  MAKVGVISWVVFIVFLAFYSGSLNTNAQLCCINHPEWGACKPGVDD-----ATDGRCWLK 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI GC KGG CK     H+CHCYC
Sbjct: 61 CIGGCEKGGICKRVGKKHVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: KGN66133.1 (hypothetical protein Csa_007601 [Cucumis sativus])

HSP 1 Score: 115.5 bits (288), Expect = 2.1e-22
Identity = 53/84 (63.10%), Postives = 62/84 (73.81%), Query Frame = 0


Query: 1  MAKVGVI-SCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G I S + +AFLVLY+GS+ T AQLCCN H ELGSC+PGVDD +     DGKCW H
Sbjct: 1  MAKLGAIFSWVFVAFLVLYAGSMNTNAQLCCNNHRELGSCVPGVDDDY-----DGKCWHH 60

Query: 61 CISGCLKGGFCKP--NNHICHCYC 82
          CI GC +GGFCK     H+CHCYC
Sbjct: 61 CIVGCERGGFCKKLWYGHVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: TYK10190.1 (putative defensin-like protein 20 [Cucumis melo var. makuwa])

HSP 1 Score: 114.8 bits (286), Expect = 3.6e-22
Identity = 55/84 (65.48%), Postives = 61/84 (72.62%), Query Frame = 0


Query: 1  MAKVGVI-SCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G I   + IAFLVLYSGS  T AQLCCN HPELGSC PGVDD       DGKCW H
Sbjct: 1  MAKLGAIFGWVFIAFLVLYSGSKNTNAQLCCNNHPELGSCEPGVDD-----ASDGKCWLH 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI+GC KGGFCK   + ++CHCYC
Sbjct: 61 CIAGCEKGGFCKQLTDKNVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: KGN66136.1 (hypothetical protein Csa_007074 [Cucumis sativus])

HSP 1 Score: 110.5 bits (275), Expect = 6.8e-21
Identity = 52/84 (61.90%), Postives = 59/84 (70.24%), Query Frame = 0


Query: 1  MAKVG-VISCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G     + IAFLVLYSGS+ T AQLCCN HPELG C  GVDD       DGKCW H
Sbjct: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDD-----ANDGKCWLH 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI+GC KGGFCK   + ++CHCYC
Sbjct: 61 CIAGCEKGGFCKHLSDKNVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: TYK10191.1 (putative defensin-like protein 20 [Cucumis melo var. makuwa])

HSP 1 Score: 97.1 bits (240), Expect = 7.7e-17
Identity = 42/63 (66.67%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 21  SLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSHCISGCLKGGFCK--PNNHICH 80
           S  T AQLCCN HPELGSC PGVDD       DGKCW HCI+GC KGGFCK   N ++CH
Sbjct: 74  SKNTNAQLCCNNHPELGSCEPGVDD-----ASDGKCWLHCIAGCEKGGFCKQLSNKNVCH 131

Query: 81  CYC 82
           CYC
Sbjct: 134 CYC 131

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: XP_010450072.1 (PREDICTED: putative defensin-like protein 20 [Camelina sativa] >XP_010450073.1 PREDICTED: putative defensin-like protein 20 [Camelina sativa] >XP_010497033.1 PREDICTED: putative defensin-like protein 20 [Camelina sativa])

HSP 1 Score: 95.5 bits (236), Expect = 2.3e-16
Identity = 45/95 (47.37%), Postives = 54/95 (56.84%), Query Frame = 0


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDKHD 60
          M    ++S  L A +VL  G  K   Q            +CC EHP LG C+PG+DDK D
Sbjct: 1  MLSTKIVSLFLFAAIVLCIGFSKVEGQKNIAPWVYNIKSICCREHPSLGRCLPGIDDKAD 60

Query: 61 NSGEDGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          N   DGKCW +C+ GC +GGFCK   N HICHCYC
Sbjct: 61 N---DGKCWKYCVEGCERGGFCKLFGNKHICHCYC 92

[Close]

BLAST of CSPI01G33230 vs. TAIR 10
Match: AT4G14276.1 (Defensin-like (DEFL) family protein )

HSP 1 Score: 80.9 bits (198), Expect = 5.3e-16
Identity = 39/95 (41.05%), Postives = 53/95 (55.79%), Query Frame = 0


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDKHD 60
          M +  V+S +L A +VL  GS++   Q            +CC EHP +G C+P +D   D
Sbjct: 1  MVRTNVVSFVLFAAIVLCIGSIQIDGQKHTAPWIPEDNSICCKEHPSVGRCLPNID---D 60

Query: 61 NSGEDGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          ++ + GKCW  CI GC  GGFCK   + HICHC C
Sbjct: 61 SAEKGGKCWKFCIEGCETGGFCKLFGHKHICHCNC 92

[Close]

BLAST of CSPI01G33230 vs. TAIR 10
Match: AT5G52605.1 (Defensin-like (DEFL) family protein )

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-15
Identity = 38/91 (41.76%), Postives = 52/91 (57.14%), Query Frame = 0


Query: 1  MAKVGVISCILIAFLVL--------YSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGE 60
          M +  V+S +L A +VL         +  +     +CC EHP +G C+PG+D   D++ +
Sbjct: 1  MVRTNVVSFVLFAAIVLCILYGQKHIAPWIFEDKSICCKEHPSVGRCLPGID---DDAEK 60

Query: 61 DGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          DGKCW  CI GC  GGFCK   + HICHC C
Sbjct: 61 DGKCWKFCIEGCETGGFCKLFEHKHICHCNC 88

[Close]

BLAST of CSPI01G33230 vs. TAIR 10
Match: AT4G18823.1 (Defensin-like (DEFL) family protein )

HSP 1 Score: 53.5 bits (127), Expect = 9.1e-08
Identity = 24/54 (44.44%), Postives = 28/54 (51.85%), Query Frame = 0


Query: 28 LCCNEHPELGSCIPGVDDKHDNSGEDGKCWSHCISGCLKGGFCKPNNHICHCYC 82
          LCCN+HPE G C             +  C   C+ GC +GGFCK    ICHCYC
Sbjct: 35 LCCNDHPEFGIC------------TNKSCNKWCLQGCTRGGFCK--RKICHCYC 74

[Close]

BLAST of CSPI01G33230 vs. TAIR 10
Match: AT5G39645.1 (Defensin-like (DEFL) family protein )

HSP 1 Score: 49.3 bits (116), Expect = 1.7e-06
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = 0


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDK 47
          M K  V+S +L A +VL  GS++   Q            +CC EHP LG C+PG+DD+
Sbjct: 1  MVKTNVVSFVLFAAIVLCIGSIQINGQKHIAPWISEEKSICCREHPSLGRCLPGIDDR 58

[Close]

BLAST of CSPI01G33230 vs. TAIR 10
Match: AT4G14272.1 (Defensin-like (DEFL) family protein )

HSP 1 Score: 43.9 bits (102), Expect = 7.2e-05
Identity = 19/47 (40.43%), Postives = 24/47 (51.06%), Query Frame = 0


Query: 29 CCNEHPELGSCIPGVDDKHDNSGEDGKCWSHCISGCLKGGFCKPNNH 76
          C  EH E+G C+ G+DD   N   DGKCW  C+         + NNH
Sbjct: 39 CNREHSEIGICLSGIDDDIQN---DGKCWKFCLMVAKAEVIMQTNNH 82

The following BLAST results are available for this feature:

BLAST of CSPI01G33230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CAX9	7.5e-15	41.05	Defensin-like protein 21 OS=Arabidopsis thaliana OX=3702 GN=At4g14276 PE=2 SV=1	[more]
Q2V2S1	1.7e-14	41.76	Putative defensin-like protein 20 OS=Arabidopsis thaliana OX=3702 GN=At5g52605 P...	[more]
Q2L6T3	1.3e-06	44.44	Putative defensin-like protein 27 OS=Arabidopsis thaliana OX=3702 GN=At4g18823 P...	[more]
P0CAY0	6.0e-04	43.90	Putative defensin-like protein 28 OS=Arabidopsis thaliana OX=3702 GN=At4g14272 P...	[more]

BLAST of CSPI01G33230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWJ9	1.0e-22	63.10	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573030 PE=3 SV=1	[more]
A0A5D3CFQ3	1.7e-22	65.48	Putative defensin-like protein 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A0A0LZ58	2.5e-21	58.82	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573070 PE=3 SV=1	[more]
A0A0A0M1N0	3.3e-21	61.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573060 PE=3 SV=1	[more]
A0A0A0LYL5	2.3e-19	60.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573040 PE=3 SV=1	[more]

BLAST of CSPI01G33230 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN66133.1	2.1e-22	63.10	hypothetical protein Csa_007601 [Cucumis sativus]	[more]
TYK10190.1	3.6e-22	65.48	putative defensin-like protein 20 [Cucumis melo var. makuwa]	[more]
KGN66136.1	6.8e-21	61.90	hypothetical protein Csa_007074 [Cucumis sativus]	[more]
TYK10191.1	7.7e-17	66.67	putative defensin-like protein 20 [Cucumis melo var. makuwa]	[more]
XP_010450072.1	2.3e-16	47.37	PREDICTED: putative defensin-like protein 20 [Camelina sativa] >XP_010450073.1 P...	[more]

BLAST of CSPI01G33230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14276.1	5.3e-16	41.05	Defensin-like (DEFL) family protein	[more]
AT5G52605.1	1.2e-15	41.76	Defensin-like (DEFL) family protein	[more]
AT4G18823.1	9.1e-08	44.44	Defensin-like (DEFL) family protein	[more]
AT5G39645.1	1.7e-06	39.66	Defensin-like (DEFL) family protein	[more]
AT4G14272.1	7.2e-05	40.43	Defensin-like (DEFL) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022618	Defensin-like protein 20-27	PFAM	PF10868	Defensin_like	coord: 28..81 e-value: 9.3E-13 score: 48.7
IPR022618	Defensin-like protein 20-27	PANTHER	PTHR34453	DEFENSIN-LIKE (DEFL) FAMILY PROTEIN-RELATED	coord: 4..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G33230.1	CSPI01G33230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
cellular_component	GO:0005576	extracellular region