CSPI01G32520 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G32520
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTryptophan synthase
LocationChr1: 27340265 .. 27344278 (+)
RNA-Seq ExpressionCSPI01G32520
SyntenyCSPI01G32520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCCTATTTATGATAACTTCGTCAAAAAGCAAAGCAAAGCAAAGCTAAACACACACACACAAAAAAAAGAAGAGAGAAGAGGAGAAAGTAGAAATTCAATAACAAAAATAAGAACAAAATTCTCTCACTCTCTCTTATAATCTCTTTTTCTCTCTAAAAATGGTTGCCTCTACGGCGCTCAACTGCAAAATCCCCACCTTTTCTCCCTCCTCTTCCCATTCAAATAACAAGATTTCTTCTTCTATCTCTCACTTTCCTGTCCGTTTCCGCCGAGTTTCCTCCAACCCATCGCCGGTTTTTCGGTCAAACGCCGTCTCCTGCACTTTGACTCGGGAGCCGTCGCTGGCAATGGAGGACAAAGTTCATGATTCGAGTCTGCAACAGAGACCGGATTCATTTGGCCGTTTTGGCCGTTTTGGGGGAAAGTATGTCCCCGAGACCCTAATGCACGCCCTTACCGAGCTCGAAACTGCTTTCTATTCCCTTGCTGGGGACCAAGACTTTCAGGTTTGTGGGAATTTTGTTTTCCTTTGAGGTTTTTTCTTCTTTATTTTTTTTAAAAAAAAAAAATAGATGGAGGAAGTTTAATTTCCTGAGTTTTGGGTCTTTGTTTTAATCGCCTAATTAGGTGCTGTTTGCGGATTTTGAATCTCTTGAATGCTTATTGGATTTTATGTGGACTTAAGTTGAATCTCTTGAATGCTTATTGGATTTTATGTGCACTTAAGTTGAAATGTTTTTCTTTTTCTTTCTTGAAATTGGAAGTAGAAAAGTGGGAGTTTGACATTTATTAGCCACAGTTACATAATAAAACCATTAAGTATGTAGATGTTATTTTCAAAGAGAAGAAGGCTTTGAGACATTTTTTTAATTTCATTGCAAAGTTCATGATTTTGAAATTCAGTAGTTTCTCAATTGGACATACCAAGCAGTACTTATTATCTTTTTTTACCCACTCTTTATAATAATTGACTCTAATCATCAGAAAGAGCTGGATGGAATTTTGAGAGACTACGTGGGGAGAGAGAGCCCTTTGTACTTCGCAGAGAGGCTGACTGAGCACTATAGACGTTCCAATGGTGAGGGGCCTCATATTTTCCTGAAGAGAGAAGACCTTAACCACACAGGGGCTCACAAAATTAACAATGCTGTTGCCCAAGCTTTGCTTGCTAAGCGTTTGGGAAAGAAACGGATTATAGCTGAAACAGGCGCAGGTCAGCATGGAGTTGCAACTGCAACTGTTTGTGCTAGATTTGGACTAGAATGTATCATCTACATGGGTGCCCAAGATATGGAAAGGCAAGCTCTCAATGTGTTCAGAATGCAGCTTCTTGGGGCTGAGGTTTGTCCATTATAATTTTGGTTGTTTTCTTCCATTATTAATCTGCAGCCATTTATTTGTAGGACTCTAATTTCATCGTCCAATCTGTTATGATCAACTTGGACCTTCAAAACTGTGAATATTGTATTTCTGTTGGAGAATTTGGGTGCTCAACACACCAGGAGAATTTATCTTATTTGTTCTCCTGGCTGAATGTTCACTTTTTTGCATATTTCAAATCCCTCTGATAACGTTTGTATTTATTACCATTCTACTGCATAGTCAGACATTCTCTTTAGTTATTCTCCTTTTTCATTGCTAGTTAGCATTCCCTAAAGTTATGGATTGAGCTTTCTCTGACTTGCATCCTTGCTATGAATATGATTTAAAGTGCAGCTTATTCAGTCAACAAATATTTATTGCATGTAATATAATTTGTTTCCTATTCTTTCCATTATTGTTTGTAGAGATAGTTTTGAACCTGGTCACAGCGTTTATATTTCTTCCAAGTCTTTTCATGAACCAGCATTTGAAAATAAAAGATTTATCCTAATAGGTGTGATTCGCAGTTGCTACCTCATCCTAGGTGTTCAAAATGAATTTCAAAGTATAAATTACACTTGCTTTGCAGGTTAGGCCAGTTCATTCTGGTACTGCCACGCTAAAGGATGCTACTTCAGAAGCTATAAGGGACTGGGTGACTAATGTGGAGACTACCCATTATATCTTAGGATCTGTTGCTGGGCCCCACCCATATCCCATGATGGTGAGAGATTTTCATGCTGTGATTGGTAAAGAAACTAGAAAACAGGCCTTGGAGAAGTGGGGAGGGAAGCCAGATGTCCTAGTAGCATGTGTTGGAGGCGGTTCAAATGCTATGGGACTATTTCATGAGTTTGTCAATGACAAAGATGTTAGGTTGGTTGGTGTGGAGGCTGCAGGTTTTGGCTTGGATAGTGGTAAGCATGCAGCCACTCTAACCAAAGGGGAAGTTGGGGTTCTACACGGAGCGATGAGTTATTTACTACAGGATGATGATGGACAAATAATTGAACCTCACTCGATCAGTGCAGGGTATGGGATATATACAAAGTTTTTCTTTGTCATGATGTTTGCCTGCTGTTCCAATCTTGCACCTTTCAAATTTAATCCATGTTTTTTTTTCATGGTCTGCAGTTTGGATTACCCTGGGGTTGGACCAGAGCATAGTTATTTGAAAGATCTAGGCCGTGCTGAGTATCACAGTGTAACTGATGATGAAGCACTGGAAGGTACTGGATTGTAATACATTAATTTGTTGGCATTTTATTTGTTAGATATACCATCTATCTTTTGAAAAGTTATCTTTTTAGATATTTATTATATATATATATATATAACTTATATATATATCCTTCTTTTTCAGAGCCAAAGTTCTCTTTTTATTCTGAAAATTACAATCTGTTTTTACTATTTAGGTAGTGTTTTATACTTATTACTTCAGTCCATTTTTTCTGCTCTGTTCAATGTTTATCTTTCTTTCCTATCTTGATTCTCACCTTCCTTTTAGGTGGAAACTGCTATGCATTCTTTTCAGCTATTAGTAATTTTGTAAACTGTGCACTGGCACATGCTAAATCATGACCAATTATCGTGGTTCTTCTTTAGGTTCCTGATGAGAAATTATCTGTTCTCATTAAGTTTTAAATAAATTTGTACTAAATTAGTTTTACATAAAGTCCTATCTGTTACTACAATCTGAGCCCTGTTTCACCCACTGATTCCATGATCAATAATCTACTGCAGAATCTATCTTGATAGTGGAGTTGTTGGTCCCTGAGGGAGGTTTCTCTTCCATGTATCTGTCTTTTGATTGATTTTGATTGTTATCTTGAATCACTATGGACTGTTTTGGAAACTTGTACATCCAATTAGAACTTGATTTCTGTCCAAGATGATGGTTGTGAAAGCTAGCTTTCACCATTTCTTGTTCTTGTTGTTCTTCTGATCCTTAGAGGCTAAATTCATAATCTGGTTTGTGACTGCAGCCTTCAAGAGATTGTCACGACTGGAGGGCATAATCCCAGCTCTGGAGACATCTCATGCTCTGGCTTATCTTGAGAAGTTGTGTCCGACCCTCCCAGACGGAACAAAGGTCGTGCTTAACTGCAGCGGCAGAGGAGATAAGGATGTTCAGACAGCGATCAAGTATTTGCAAGTTTGATACATCACCATGGCTACCAGTACTAGTAAAACTAGAGGTCATTTTCTCTTGGTGAAAGCTTCGTCTAAATTGAATTGTTTTCCAATATGATGAGATCCGAGCTTGAAGACAATAAAGCATACATTACAAAGCAAAGTTTCCTTTCCTTGCCTTTCCTTTCTTTTAGAAACTATATGTTGTATTTATACAAGTAAAGTTTGCATTTTGATACAGATTCTGGCGAAATGAATAGTCTGAAGAGGCCTTGTGGGGATAGATTTTACTGTTTATGCATCTTTTTGAGGTTTTAATGAATGAGTCTGTATCAAGTGTTTGGACTTATTACATAAGAATAAGTTAGACAAACAGTGTAACTTGTGTTTGGACTGATATGTGTTGTATAGAAAGTGGGAGTGGGATCTACAAACCTACTTTCACGTGGACAAACAATATATAAAAGAGTGTTTAACTTATATAGCCTAAATTGATATATGATATCACGTGAAAGACC

mRNA sequence

GCCCTATTTATGATAACTTCGTCAAAAAGCAAAGCAAAGCAAAGCTAAACACACACACACAAAAAAAAGAAGAGAGAAGAGGAGAAAGTAGAAATTCAATAACAAAAATAAGAACAAAATTCTCTCACTCTCTCTTATAATCTCTTTTTCTCTCTAAAAATGGTTGCCTCTACGGCGCTCAACTGCAAAATCCCCACCTTTTCTCCCTCCTCTTCCCATTCAAATAACAAGATTTCTTCTTCTATCTCTCACTTTCCTGTCCGTTTCCGCCGAGTTTCCTCCAACCCATCGCCGGTTTTTCGGTCAAACGCCGTCTCCTGCACTTTGACTCGGGAGCCGTCGCTGGCAATGGAGGACAAAGTTCATGATTCGAGTCTGCAACAGAGACCGGATTCATTTGGCCGTTTTGGCCGTTTTGGGGGAAAGTATGTCCCCGAGACCCTAATGCACGCCCTTACCGAGCTCGAAACTGCTTTCTATTCCCTTGCTGGGGACCAAGACTTTCAGAAAGAGCTGGATGGAATTTTGAGAGACTACGTGGGGAGAGAGAGCCCTTTGTACTTCGCAGAGAGGCTGACTGAGCACTATAGACGTTCCAATGGTGAGGGGCCTCATATTTTCCTGAAGAGAGAAGACCTTAACCACACAGGGGCTCACAAAATTAACAATGCTGTTGCCCAAGCTTTGCTTGCTAAGCGTTTGGGAAAGAAACGGATTATAGCTGAAACAGGCGCAGGTCAGCATGGAGTTGCAACTGCAACTGTTTGTGCTAGATTTGGACTAGAATGTATCATCTACATGGGTGCCCAAGATATGGAAAGGCAAGCTCTCAATGTGTTCAGAATGCAGCTTCTTGGGGCTGAGGTTAGGCCAGTTCATTCTGGTACTGCCACGCTAAAGGATGCTACTTCAGAAGCTATAAGGGACTGGGTGACTAATGTGGAGACTACCCATTATATCTTAGGATCTGTTGCTGGGCCCCACCCATATCCCATGATGGTGAGAGATTTTCATGCTGTGATTGGTAAAGAAACTAGAAAACAGGCCTTGGAGAAGTGGGGAGGGAAGCCAGATGTCCTAGTAGCATGTGTTGGAGGCGGTTCAAATGCTATGGGACTATTTCATGAGTTTGTCAATGACAAAGATGTTAGGTTGGTTGGTGTGGAGGCTGCAGGTTTTGGCTTGGATAGTGGTAAGCATGCAGCCACTCTAACCAAAGGGGAAGTTGGGGTTCTACACGGAGCGATGAGTTATTTACTACAGGATGATGATGGACAAATAATTGAACCTCACTCGATCAGTGCAGGTTTGGATTACCCTGGGGTTGGACCAGAGCATAGTTATTTGAAAGATCTAGGCCGTGCTGAGTATCACAGTGTAACTGATGATGAAGCACTGGAAGCCTTCAAGAGATTGTCACGACTGGAGGGCATAATCCCAGCTCTGGAGACATCTCATGCTCTGGCTTATCTTGAGAAGTTGTGTCCGACCCTCCCAGACGGAACAAAGGTCGTGCTTAACTGCAGCGGCAGAGGAGATAAGGATGTTCAGACAGCGATCAAGTATTTGCAAGTTTGATACATCACCATGGCTACCAGTACTAGTAAAACTAGAGGTCATTTTCTCTTGGTGAAAGCTTCGTCTAAATTGAATTGTTTTCCAATATGATGAGATCCGAGCTTGAAGACAATAAAGCATACATTACAAAGCAAAGTTTCCTTTCCTTGCCTTTCCTTTCTTTTAGAAACTATATGTTGTATTTATACAAGTAAAGTTTGCATTTTGATACAGATTCTGGCGAAATGAATAGTCTGAAGAGGCCTTGTGGGGATAGATTTTACTGTTTATGCATCTTTTTGAGGTTTTAATGAATGAGTCTGTATCAAGTGTTTGGACTTATTACATAAGAATAAGTTAGACAAACAGTGTAACTTGTGTTTGGACTGATATGTGTTGTATAGAAAGTGGGAGTGGGATCTACAAACCTACTTTCACGTGGACAAACAATATATAAAAGAGTGTTTAACTTATATAGCCTAAATTGATATATGATATCACGTGAAAGACC

Coding sequence (CDS)

ATGGTTGCCTCTACGGCGCTCAACTGCAAAATCCCCACCTTTTCTCCCTCCTCTTCCCATTCAAATAACAAGATTTCTTCTTCTATCTCTCACTTTCCTGTCCGTTTCCGCCGAGTTTCCTCCAACCCATCGCCGGTTTTTCGGTCAAACGCCGTCTCCTGCACTTTGACTCGGGAGCCGTCGCTGGCAATGGAGGACAAAGTTCATGATTCGAGTCTGCAACAGAGACCGGATTCATTTGGCCGTTTTGGCCGTTTTGGGGGAAAGTATGTCCCCGAGACCCTAATGCACGCCCTTACCGAGCTCGAAACTGCTTTCTATTCCCTTGCTGGGGACCAAGACTTTCAGAAAGAGCTGGATGGAATTTTGAGAGACTACGTGGGGAGAGAGAGCCCTTTGTACTTCGCAGAGAGGCTGACTGAGCACTATAGACGTTCCAATGGTGAGGGGCCTCATATTTTCCTGAAGAGAGAAGACCTTAACCACACAGGGGCTCACAAAATTAACAATGCTGTTGCCCAAGCTTTGCTTGCTAAGCGTTTGGGAAAGAAACGGATTATAGCTGAAACAGGCGCAGGTCAGCATGGAGTTGCAACTGCAACTGTTTGTGCTAGATTTGGACTAGAATGTATCATCTACATGGGTGCCCAAGATATGGAAAGGCAAGCTCTCAATGTGTTCAGAATGCAGCTTCTTGGGGCTGAGGTTAGGCCAGTTCATTCTGGTACTGCCACGCTAAAGGATGCTACTTCAGAAGCTATAAGGGACTGGGTGACTAATGTGGAGACTACCCATTATATCTTAGGATCTGTTGCTGGGCCCCACCCATATCCCATGATGGTGAGAGATTTTCATGCTGTGATTGGTAAAGAAACTAGAAAACAGGCCTTGGAGAAGTGGGGAGGGAAGCCAGATGTCCTAGTAGCATGTGTTGGAGGCGGTTCAAATGCTATGGGACTATTTCATGAGTTTGTCAATGACAAAGATGTTAGGTTGGTTGGTGTGGAGGCTGCAGGTTTTGGCTTGGATAGTGGTAAGCATGCAGCCACTCTAACCAAAGGGGAAGTTGGGGTTCTACACGGAGCGATGAGTTATTTACTACAGGATGATGATGGACAAATAATTGAACCTCACTCGATCAGTGCAGGTTTGGATTACCCTGGGGTTGGACCAGAGCATAGTTATTTGAAAGATCTAGGCCGTGCTGAGTATCACAGTGTAACTGATGATGAAGCACTGGAAGCCTTCAAGAGATTGTCACGACTGGAGGGCATAATCCCAGCTCTGGAGACATCTCATGCTCTGGCTTATCTTGAGAAGTTGTGTCCGACCCTCCCAGACGGAACAAAGGTCGTGCTTAACTGCAGCGGCAGAGGAGATAAGGATGTTCAGACAGCGATCAAGTATTTGCAAGTTTGA

Protein sequence

MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREPSLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELDGILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV*
Homology
[Close]
BLAST of CSPI01G32520 vs. ExPASy Swiss-Prot
Match: P14671 (Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 4.8e-223
Identity = 387/464 (83.41%), Postives = 417/464 (89.87%), Query Frame = 0 

Query: 13  TFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSN----AVSCTLTREPSLAMEDKV 72
           TF  S S + +  SS ++H    F+ V   P P  RS     +VSCT+ ++P + M    
Sbjct: 9   TFRASVSSAPSS-SSQLTHLKSPFKAVKYTPLPSSRSKSSSFSVSCTIAKDPPVLMA-AG 68

Query: 73  HDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELDGILRDYVG 132
            D +L QRPDSFGRFG+FGGKYVPETLMHAL+ELE+AFY+LA D DFQ+EL GIL+DYVG
Sbjct: 69  SDPALWQRPDSFGRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVG 128

Query: 133 RESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 192
           RESPLYFAERLTEHYRR NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA
Sbjct: 129 RESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 188

Query: 193 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVHSGTATLKD 252
           ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVR VHSGTATLKD
Sbjct: 189 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKD 248

Query: 253 ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV 312
           ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV
Sbjct: 249 ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV 308

Query: 313 ACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQ 372
           ACVGGGSNAMGLFHEFVND +VR++GVEAAGFGLDSGKHAATLTKG+VGVLHGAMSYLLQ
Sbjct: 309 ACVGGGSNAMGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQ 368

Query: 373 DDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPA 432
           DDDGQIIEPHSISAGLDYPGVGPEHS+ KD+GRAEY+S+TD+EALEAFKR+SRLEGIIPA
Sbjct: 369 DDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEGIIPA 428

Query: 433 LETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           LETSHALAYLEKLCPTL DGT+VVLN SGRGDKDVQT  KYL V
Sbjct: 429 LETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKYLDV 470

[Close]
BLAST of CSPI01G32520 vs. ExPASy Swiss-Prot
Match: P25269 (Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB2 PE=1 SV=2)

HSP 1 Score: 773.9 bits (1997), Expect = 1.1e-222
Identity = 386/469 (82.30%), Postives = 425/469 (90.62%), Query Frame = 0 

Query: 13  TFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSN---AVSCTLTREPSLAMEDKV- 72
           TF PSS  ++++++   S  P +  + +  PS   RS+   +VSCT+ ++P++ M D   
Sbjct: 9   TFRPSSVSASSELTHLRS--PSKLPKFTPLPSARSRSSSSFSVSCTIAKDPAVVMADSEK 68

Query: 73  -----HDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELDGIL 132
                 D ++ QRPDSFGRFG+FGGKYVPETLMHAL+ELETAFYSLA D+DFQ+EL  IL
Sbjct: 69  IKAAGSDPTMWQRPDSFGRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEIL 128

Query: 133 RDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGK 192
           +DYVGRESPLYFAERLTEHYRR NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGK
Sbjct: 129 KDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGK 188

Query: 193 KRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVHSGT 252
           KRIIAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQALNVFRM+LLGAEVR VHSGT
Sbjct: 189 KRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGT 248

Query: 253 ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGK 312
           ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQA+EKWGGK
Sbjct: 249 ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGK 308

Query: 313 PDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAM 372
           PDVLVACVGGGSNAMGLFHEFV+D +VR++GVEAAGFGLDSGKHAATLTKG+VGVLHGAM
Sbjct: 309 PDVLVACVGGGSNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKGDVGVLHGAM 368

Query: 373 SYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLE 432
           SYLLQDDDGQIIEPHSISAGLDYPGVGPEHS+LKD+GRAEY SVTD+EALEAFKR+SRLE
Sbjct: 369 SYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLE 428

Query: 433 GIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           GIIPALETSHALA+LEKLCPTLPDG +VVLN SGRGDKDVQTAIKYL+V
Sbjct: 429 GIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKYLEV 475

[Close]
BLAST of CSPI01G32520 vs. ExPASy Swiss-Prot
Match: O50046 (Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=16922 GN=TSB PE=2 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 5.0e-220
Identity = 381/466 (81.76%), Postives = 415/466 (89.06%), Query Frame = 0 

Query: 20  HSNNKISSSISHFPVRFRRVSSNPS-------------PVFRSNAVSCTLTREPSLAMED 79
           ++N    ++ S FP  +R   SNPS                +  ++ CT+ RE  +  E 
Sbjct: 4   YTNPACRTNTSAFPGPYRPY-SNPSRFSFNLDKFRPRTSAIKVPSICCTIARE--MEKER 63

Query: 80  KVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELDGILRDY 139
              +  + QRPDSFGRFG+FGGKYVPETLM+ALTELE+AF SL+GDQ FQKELDGIL+DY
Sbjct: 64  SEREPDVLQRPDSFGRFGKFGGKYVPETLMYALTELESAFRSLSGDQVFQKELDGILKDY 123

Query: 140 VGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRI 199
           VGRESPLYFAERLT HY+R NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGKKRI
Sbjct: 124 VGRESPLYFAERLTLHYKRPNGEGPEIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRI 183

Query: 200 IAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVHSGTATL 259
           IAETGAGQHGVATATVCARFGL+C+IYMGAQDMERQALNVFRM+LLGAEVR VHSGTATL
Sbjct: 184 IAETGAGQHGVATATVCARFGLQCVIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATL 243

Query: 260 KDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDV 319
           KDATSEAIRDWVTNVE+THYILGSVAGPHPYPMMVR+FHAVIGKETRKQALEKWGGKPDV
Sbjct: 244 KDATSEAIRDWVTNVESTHYILGSVAGPHPYPMMVREFHAVIGKETRKQALEKWGGKPDV 303

Query: 320 LVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYL 379
           LVACVGGGSNAMGLFHEFV+DKDVR++GVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYL
Sbjct: 304 LVACVGGGSNAMGLFHEFVDDKDVRMIGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYL 363

Query: 380 LQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGII 439
           LQDDDGQIIEPHSISAGLDYPGVGPEHS+LKD+GRAEY+  TD+EALEAFKRLSRLEGII
Sbjct: 364 LQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDIGRAEYYCCTDEEALEAFKRLSRLEGII 423

Query: 440 PALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           PALETSHALA+LEKLCPTLP+GTKVVLNCSGRGDKDV TAIK+LQV
Sbjct: 424 PALETSHALAFLEKLCPTLPNGTKVVLNCSGRGDKDVHTAIKHLQV 466

[Close]
BLAST of CSPI01G32520 vs. ExPASy Swiss-Prot
Match: P43284 (Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 GN=TSB2 PE=2 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 1.0e-209
Identity = 350/398 (87.94%), Postives = 380/398 (95.48%), Query Frame = 0 

Query: 75  QRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELDGILRDYVGRESPLY 134
           QRPD+ GRFGRFGGKYVPETLMHALTELE+AF++LA D +FQKELDGIL+DYVGRESPLY
Sbjct: 46  QRPDAMGRFGRFGGKYVPETLMHALTELESAFHALATDDEFQKELDGILKDYVGRESPLY 105

Query: 135 FAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQ 194
           FAERLTEHY+R++G GP I+LKREDLNHTGAHKINNAVAQALLAKRLGK+RIIAETGAGQ
Sbjct: 106 FAERLTEHYKRADGTGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKQRIIAETGAGQ 165

Query: 195 HGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVHSGTATLKDATSEAI 254
           HGVATATVC RFGL+CIIYMGAQDMERQALNVFRM+LLGAEVR VHSGTATLKDATSEAI
Sbjct: 166 HGVATATVCRRFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATLKDATSEAI 225

Query: 255 RDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGG 314
           RDWVTNVETTHYILGSVAGPHPYPMMVR+FH VIGKETR+QA++KWGGKPDVLVACVGGG
Sbjct: 226 RDWVTNVETTHYILGSVAGPHPYPMMVREFHKVIGKETRRQAMDKWGGKPDVLVACVGGG 285

Query: 315 SNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDGQI 374
           SNAMGLFHEFV D+DVRLVG+EAAG G+D+ KHAATLTKG+VGVLHG+MSYLLQDDDGQ+
Sbjct: 286 SNAMGLFHEFVEDQDVRLVGLEAAGHGVDTDKHAATLTKGQVGVLHGSMSYLLQDDDGQV 345

Query: 375 IEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPALETSHA 434
           IEPHSISAGLDYPGVGPEHS+LKD+GRAEY SVTD EAL+AFKR+SRLEGIIPALETSHA
Sbjct: 346 IEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLEGIIPALETSHA 405

Query: 435 LAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           LAYLEKLCPTL DG +VV+NCSGRGDKDV TA KYL V
Sbjct: 406 LAYLEKLCPTLADGVRVVVNCSGRGDKDVHTASKYLDV 443

[Close]
BLAST of CSPI01G32520 vs. ExPASy Swiss-Prot
Match: P43283 (Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 5.4e-206
Identity = 345/389 (88.69%), Postives = 371/389 (95.37%), Query Frame = 0 

Query: 84  GRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELDGILRDYVGRESPLYFAERLTEHY 143
           GRFGGKYVPETLMHALTELE AF++LA D +FQKELDGIL+DYVGRESPLYFAERLTEHY
Sbjct: 1   GRFGGKYVPETLMHALTELENAFHALATDDEFQKELDGILKDYVGRESPLYFAERLTEHY 60

Query: 144 RRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVC 203
           +R++G GP I+LKREDLNH GAHKINNAVAQALLAKRLGK+RIIAETGAGQHGVATATVC
Sbjct: 61  KRADGTGPLIYLKREDLNHRGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATATVC 120

Query: 204 ARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVHSGTATLKDATSEAIRDWVTNVET 263
           ARFGL+CIIYMGAQDMERQALNVFRM+LLGAEVR VHSGTATLKDATSEAIRDWVTNVET
Sbjct: 121 ARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGTATLKDATSEAIRDWVTNVET 180

Query: 264 THYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNAMGLFHE 323
           THYILGSVAGPHPYPMMVR+FH VIGKETR+QA+ KWGGKPDVLVACVGGGSNAMGLFHE
Sbjct: 181 THYILGSVAGPHPYPMMVREFHKVIGKETRRQAMHKWGGKPDVLVACVGGGSNAMGLFHE 240

Query: 324 FVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDGQIIEPHSISAG 383
           FV D+DVRL+GVEAAG G+D+ KHAATLTKG+VGVLHG+MSYLLQDDDGQ+IEPHSISAG
Sbjct: 241 FVEDQDVRLIGVEAAGHGVDTDKHAATLTKGQVGVLHGSMSYLLQDDDGQVIEPHSISAG 300

Query: 384 LDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPALETSHALAYLEKLCP 443
           LDYPGVGPEHS+LKD+GRAEY SVTD EAL+AFKR+SRLEGIIPALETSHALAYLEKLCP
Sbjct: 301 LDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLEGIIPALETSHALAYLEKLCP 360

Query: 444 TLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           TLPDG +VVLNCSGRGDKDV TA KYL V
Sbjct: 361 TLPDGVRVVLNCSGRGDKDVHTASKYLDV 389

[Close]
BLAST of CSPI01G32520 vs. ExPASy TrEMBL
Match: A0A0A0M065 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_1G660140 PE=3 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 1.8e-273
Identity = 470/472 (99.58%), Postives = 471/472 (99.79%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPTFSPSSSHSNNKISSS SHFPVRFRRVSSNPSPVFRSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTFSPSSSHSNNKISSSTSHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD
Sbjct: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRL+
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLA 420

Query: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472

[Close]
BLAST of CSPI01G32520 vs. ExPASy TrEMBL
Match: A0A1S3C016 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103494920 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 3.9e-268
Identity = 461/472 (97.67%), Postives = 465/472 (98.52%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPS SHSNNKISSSI HFPVRFRRVSSNPSP+ RSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSPSHSNNKISSSIPHFPVRFRRVSSNPSPLLRSNAVSCTLTREP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDK+H  SLQQRPDSFGRFGRFGGKYVPETLMHALTELE AFYSLAGDQDFQKELD
Sbjct: 61  SLAMEDKLHTLSLQQRPDSFGRFGRFGGKYVPETLMHALTELEAAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472

[Close]
BLAST of CSPI01G32520 vs. ExPASy TrEMBL
Match: A0A5D3E225 (Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold588G00030 PE=3 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.4e-265
Identity = 461/483 (95.45%), Postives = 465/483 (96.27%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPS SHSNNKISSSI HFPVRFRRVSSNPSP+ RSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSPSHSNNKISSSIPHFPVRFRRVSSNPSPLLRSNAVSCTLTREP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDK+H  SLQQRPDSFGRFGRFGGKYVPETLMHALTELE AFYSLAGDQDFQKELD
Sbjct: 61  SLAMEDKLHTLSLQQRPDSFGRFGRFGGKYVPETLMHALTELEAAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALE------ 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALE      
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEEGKFII 420

Query: 421 -----AFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKY 473
                AFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKY
Sbjct: 421 WLVTAAFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKY 480

[Close]
BLAST of CSPI01G32520 vs. ExPASy TrEMBL
Match: A0A6J1CDB2 (Tryptophan synthase OS=Momordica charantia OX=3673 GN=LOC111010430 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.1e-251
Identity = 436/472 (92.37%), Postives = 448/472 (94.92%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVA TALNCKIPT SPSSSHSN++ISSS S  P+RFRR +S PSP  R   VSCTLT +P
Sbjct: 1   MVAFTALNCKIPTVSPSSSHSNSEISSSFSRLPLRFRRAASYPSPAARRCGVSCTLTTQP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDK       QRPDSFGRFGRFGGKYVPETLMHALT+LE AF+SLAGDQ+FQKELD
Sbjct: 61  SLAMEDKTQ----TQRPDSFGRFGRFGGKYVPETLMHALTQLEAAFHSLAGDQEFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRR NGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRPNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEV+PVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVKPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFV DKDVRLVGVEAAGFGLDSGKHAATLTKGE+GVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVGDKDVRLVGVEAAGFGLDSGKHAATLTKGEIGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEY+SVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYYSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDV TAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVHTAIKYLQV 468

[Close]
BLAST of CSPI01G32520 vs. ExPASy TrEMBL
Match: A0A6J1J261 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111480575 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.1e-249
Identity = 434/472 (91.95%), Postives = 446/472 (94.49%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVAS  L+CKI T  PSSSHSN+KISSS S FPV  RR   NPSPVFRS AV+CTLTRE 
Sbjct: 1   MVASVELSCKILTIPPSSSHSNSKISSSFSRFPVHSRRFLQNPSPVFRSCAVTCTLTRET 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMED     +L QRPDSFGRFGRFGGKYVPETLMHAL ELETAF+SL+GDQ+FQKELD
Sbjct: 61  SLAMEDN-KTQNLGQRPDSFGRFGRFGGKYVPETLMHALAELETAFHSLSGDQEFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGR SPLYFAERLTEHYRR NGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRASPLYFAERLTEHYRRPNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFG+DSGKHAATLTKGE+GVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGVDSGKHAATLTKGEIGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKD GRAEY+SVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDAGRAEYYSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLCPTL DGTKVVLNCSGRGDKDV TAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLADGTKVVLNCSGRGDKDVHTAIKYLQV 471

[Close]
BLAST of CSPI01G32520 vs. NCBI nr
Match: XP_004142558.1 (tryptophan synthase beta chain 1 [Cucumis sativus] >KGN66694.1 hypothetical protein Csa_007090 [Cucumis sativus])

HSP 1 Score: 951.0 bits (2457), Expect = 3.7e-273
Identity = 470/472 (99.58%), Postives = 471/472 (99.79%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPTFSPSSSHSNNKISSS SHFPVRFRRVSSNPSPVFRSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTFSPSSSHSNNKISSSTSHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD
Sbjct: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRL+
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLA 420

Query: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472

[Close]
BLAST of CSPI01G32520 vs. NCBI nr
Match: XP_008454527.1 (PREDICTED: tryptophan synthase beta chain 1 [Cucumis melo])

HSP 1 Score: 933.3 bits (2411), Expect = 8.1e-268
Identity = 461/472 (97.67%), Postives = 465/472 (98.52%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPS SHSNNKISSSI HFPVRFRRVSSNPSP+ RSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSPSHSNNKISSSIPHFPVRFRRVSSNPSPLLRSNAVSCTLTREP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDK+H  SLQQRPDSFGRFGRFGGKYVPETLMHALTELE AFYSLAGDQDFQKELD
Sbjct: 61  SLAMEDKLHTLSLQQRPDSFGRFGRFGGKYVPETLMHALTELEAAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 472

[Close]
BLAST of CSPI01G32520 vs. NCBI nr
Match: KAA0053097.1 (tryptophan synthase beta chain 1 [Cucumis melo var. makuwa] >TYK29942.1 tryptophan synthase beta chain 1 [Cucumis melo var. makuwa])

HSP 1 Score: 924.9 bits (2389), Expect = 2.9e-265
Identity = 461/483 (95.45%), Postives = 465/483 (96.27%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPS SHSNNKISSSI HFPVRFRRVSSNPSP+ RSNAVSCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSPSHSNNKISSSIPHFPVRFRRVSSNPSPLLRSNAVSCTLTREP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDK+H  SLQQRPDSFGRFGRFGGKYVPETLMHALTELE AFYSLAGDQDFQKELD
Sbjct: 61  SLAMEDKLHTLSLQQRPDSFGRFGRFGGKYVPETLMHALTELEAAFYSLAGDQDFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALE------ 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALE      
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEEGKFII 420

Query: 421 -----AFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKY 473
                AFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKY
Sbjct: 421 WLVTAAFKRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKY 480

[Close]
BLAST of CSPI01G32520 vs. NCBI nr
Match: XP_038899607.1 (tryptophan synthase beta chain 1 [Benincasa hispida])

HSP 1 Score: 917.5 bits (2370), Expect = 4.6e-263
Identity = 452/472 (95.76%), Postives = 461/472 (97.67%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVASTALNCKIPT SPSSSHSN KISSS S FPV FRRV SNPSPVFRSNA+SCTLTREP
Sbjct: 1   MVASTALNCKIPTISPSSSHSNTKISSSNSRFPVHFRRVLSNPSPVFRSNAISCTLTREP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDK+HD +L+QRPDSFG FGRFGGKYVPETLMHALTELETAF+SLAGDQ+FQKELD
Sbjct: 61  SLAMEDKLHDLNLEQRPDSFGHFGRFGGKYVPETLMHALTELETAFHSLAGDQEFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRR NGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRPNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDM+RQALNVFRM+LLGAEVRPVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMDRQALNVFRMRLLGAEVRPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GG PDVLVACVGGGSNAMGLFHEFVND+DVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH
Sbjct: 301 GGTPDVLVACVGGGSNAMGLFHEFVNDEDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLCPTLPDG KVVLNCSGRGDKDVQTAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGAKVVLNCSGRGDKDVQTAIKYLQV 472

[Close]
BLAST of CSPI01G32520 vs. NCBI nr
Match: XP_022139544.1 (tryptophan synthase beta chain 1 [Momordica charantia])

HSP 1 Score: 878.6 bits (2269), Expect = 2.4e-251
Identity = 436/472 (92.37%), Postives = 448/472 (94.92%), Query Frame = 0 

Query: 1   MVASTALNCKIPTFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSNAVSCTLTREP 60
           MVA TALNCKIPT SPSSSHSN++ISSS S  P+RFRR +S PSP  R   VSCTLT +P
Sbjct: 1   MVAFTALNCKIPTVSPSSSHSNSEISSSFSRLPLRFRRAASYPSPAARRCGVSCTLTTQP 60

Query: 61  SLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELD 120
           SLAMEDK       QRPDSFGRFGRFGGKYVPETLMHALT+LE AF+SLAGDQ+FQKELD
Sbjct: 61  SLAMEDKTQ----TQRPDSFGRFGRFGGKYVPETLMHALTQLEAAFHSLAGDQEFQKELD 120

Query: 121 GILRDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180
           GILRDYVGRESPLYFAERLTEHYRR NGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR
Sbjct: 121 GILRDYVGRESPLYFAERLTEHYRRPNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKR 180

Query: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVH 240
           LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEV+PVH
Sbjct: 181 LGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVKPVH 240

Query: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300
           SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW
Sbjct: 241 SGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKW 300

Query: 301 GGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLH 360
           GGKPDVLVACVGGGSNAMGLFHEFV DKDVRLVGVEAAGFGLDSGKHAATLTKGE+GVLH
Sbjct: 301 GGKPDVLVACVGGGSNAMGLFHEFVGDKDVRLVGVEAAGFGLDSGKHAATLTKGEIGVLH 360

Query: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLS 420
           GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEY+SVTDDEALEAFKRLS
Sbjct: 361 GAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYYSVTDDEALEAFKRLS 420

Query: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDV TAIKYLQV
Sbjct: 421 RLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVHTAIKYLQV 468

[Close]
BLAST of CSPI01G32520 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 775.0 bits (2000), Expect = 3.4e-224
Identity = 387/464 (83.41%), Postives = 417/464 (89.87%), Query Frame = 0 

Query: 13  TFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSN----AVSCTLTREPSLAMEDKV 72
           TF  S S + +  SS ++H    F+ V   P P  RS     +VSCT+ ++P + M    
Sbjct: 9   TFRASVSSAPSS-SSQLTHLKSPFKAVKYTPLPSSRSKSSSFSVSCTIAKDPPVLMA-AG 68

Query: 73  HDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELDGILRDYVG 132
            D +L QRPDSFGRFG+FGGKYVPETLMHAL+ELE+AFY+LA D DFQ+EL GIL+DYVG
Sbjct: 69  SDPALWQRPDSFGRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVG 128

Query: 133 RESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 192
           RESPLYFAERLTEHYRR NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA
Sbjct: 129 RESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 188

Query: 193 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVHSGTATLKD 252
           ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRM+LLGAEVR VHSGTATLKD
Sbjct: 189 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKD 248

Query: 253 ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV 312
           ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV
Sbjct: 249 ATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLV 308

Query: 313 ACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQ 372
           ACVGGGSNAMGLFHEFVND +VR++GVEAAGFGLDSGKHAATLTKG+VGVLHGAMSYLLQ
Sbjct: 309 ACVGGGSNAMGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQ 368

Query: 373 DDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLEGIIPA 432
           DDDGQIIEPHSISAGLDYPGVGPEHS+ KD+GRAEY+S+TD+EALEAFKR+SRLEGIIPA
Sbjct: 369 DDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEGIIPA 428

Query: 433 LETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           LETSHALAYLEKLCPTL DGT+VVLN SGRGDKDVQT  KYL V
Sbjct: 429 LETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKYLDV 470

[Close]
BLAST of CSPI01G32520 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 773.9 bits (1997), Expect = 7.6e-224
Identity = 386/469 (82.30%), Postives = 425/469 (90.62%), Query Frame = 0 

Query: 13  TFSPSSSHSNNKISSSISHFPVRFRRVSSNPSPVFRSN---AVSCTLTREPSLAMEDKV- 72
           TF PSS  ++++++   S  P +  + +  PS   RS+   +VSCT+ ++P++ M D   
Sbjct: 9   TFRPSSVSASSELTHLRS--PSKLPKFTPLPSARSRSSSSFSVSCTIAKDPAVVMADSEK 68

Query: 73  -----HDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKELDGIL 132
                 D ++ QRPDSFGRFG+FGGKYVPETLMHAL+ELETAFYSLA D+DFQ+EL  IL
Sbjct: 69  IKAAGSDPTMWQRPDSFGRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEIL 128

Query: 133 RDYVGRESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGK 192
           +DYVGRESPLYFAERLTEHYRR NGEGP I+LKREDLNHTGAHKINNAVAQALLAKRLGK
Sbjct: 129 KDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGK 188

Query: 193 KRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPVHSGT 252
           KRIIAETGAGQHGVATATVCARFGL+CIIYMGAQDMERQALNVFRM+LLGAEVR VHSGT
Sbjct: 189 KRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGT 248

Query: 253 ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGK 312
           ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQA+EKWGGK
Sbjct: 249 ATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGK 308

Query: 313 PDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEVGVLHGAM 372
           PDVLVACVGGGSNAMGLFHEFV+D +VR++GVEAAGFGLDSGKHAATLTKG+VGVLHGAM
Sbjct: 309 PDVLVACVGGGSNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKGDVGVLHGAM 368

Query: 373 SYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAFKRLSRLE 432
           SYLLQDDDGQIIEPHSISAGLDYPGVGPEHS+LKD+GRAEY SVTD+EALEAFKR+SRLE
Sbjct: 369 SYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLE 428

Query: 433 GIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIKYLQV 473
           GIIPALETSHALA+LEKLCPTLPDG +VVLN SGRGDKDVQTAIKYL+V
Sbjct: 429 GIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKYLEV 475

[Close]
BLAST of CSPI01G32520 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 547.7 bits (1410), Expect = 8.9e-156
Identity = 266/412 (64.56%), Postives = 327/412 (79.37%), Query Frame = 0 

Query: 60  PSLAMEDKVHDSSLQQRPDSFGRFGRFGGKYVPETLMHALTELETAFYSLAGDQDFQKEL 119
           PSL       D     R D  G+FGRFGGK+VPETLM  L ELE  F  +  D +FQ+EL
Sbjct: 49  PSLDSVPTRTDKRQFLRGDGNGKFGRFGGKFVPETLMSRLIELEDEFNFVRCDHEFQEEL 108

Query: 120 DGILRDYVGRESPLYFAERLTEHYRR---SNGEGPHIFLKREDLNHTGAHKINNAVAQAL 179
              LRDYVGRE+PLYFAERLTEHY+    +   GP I+LKREDL+H G+HKINNA+AQA+
Sbjct: 109 TTALRDYVGRETPLYFAERLTEHYKNIVPTIEGGPEIYLKREDLSHCGSHKINNALAQAM 168

Query: 180 LAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEV 239
           +++RLG  R++A TGAGQHGVATA  CA+  LEC ++MGA D+E+Q+ NV  M+LLGA+V
Sbjct: 169 ISRRLGCSRVVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLLGAQV 228

Query: 240 RPVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQA 299
             V     T KDA+SEAIR+WV N+ TT+Y+ G+V GPHP P++VR+F +VIGKETR+QA
Sbjct: 229 ISVE---GTFKDASSEAIRNWVENLYTTYYLSGTVVGPHPCPIIVREFQSVIGKETRRQA 288

Query: 300 LEKWGGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTKGEV 359
            + WGGKPDVLVACVG GSNA+GLFHEFV D+DVRLVGVEAAG GLDSGKH+ATL  G+V
Sbjct: 289 KQLWGGKPDVLVACVGSGSNALGLFHEFVGDEDVRLVGVEAAGLGLDSGKHSATLAFGDV 348

Query: 360 GVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSYLKDLGRAEYHSVTDDEALEAF 419
           GV HG+MSYLLQDD GQI++PHS+  GL+YPGVGPE S++K+ GRAE+++ TD+EA++A 
Sbjct: 349 GVYHGSMSYLLQDDQGQILKPHSVGVGLEYPGVGPEISFMKETGRAEFYTATDEEAIQAC 408

Query: 420 KRLSRLEGIIPALETSHALAYLEKLCPTLPDGTKVVLNCSGRGDKDVQTAIK 469
            RLSRLEGIIPALE SHALA+L+KL PTL DG KVV+NCSGRGDKD+ T I+
Sbjct: 409 MRLSRLEGIIPALEASHALAFLDKLVPTLRDGAKVVVNCSGRGDKDLDTLIQ 457

[Close]
BLAST of CSPI01G32520 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-29
Identity = 111/366 (30.33%), Postives = 161/366 (43.99%), Query Frame = 0 

Query: 129 RESPLYFAERLTEHYRRSNGEGPHIFLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIA 188
           R +PL  A+RL +  +        I+ K E  +  G+HK N AV QA    + G K ++ 
Sbjct: 132 RPTPLIRAKRLEKLLQTP----ARIYFKYEGGSPAGSHKPNTAVPQAYYNAKEGVKNVVT 191

Query: 189 ETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMQLLGAEVRPV--------- 248
           ETGAGQ G + A   + FGL+C ++  A     +      MQ  GA+V P          
Sbjct: 192 ETGAGQWGSSLAFASSLFGLDCEVWQVANSYHTKPYRRLMMQTWGAKVHPSPSDLTEAGR 251

Query: 249 ------HSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETR 308
                  S   +L  A SEA+     N E T Y LGSV         V     +IG+E  
Sbjct: 252 RILESDPSSPGSLGIAISEAVEVAARN-EDTKYCLGSVLN------HVLLHQTIIGEECI 311

Query: 309 KQALEKWGGKPDVLVACVGGGSNAMGLFHEFVNDKDVRLVGVEAAGFGLDSGKHAATLTK 368
           +Q +E +G  PD+++ C GGGSN  GL   F+ +K   L G               +LTK
Sbjct: 312 QQ-MENFGETPDLIIGCTGGGSNFAGLSFPFIREK---LKGKINPVIRAVEPSACPSLTK 371

Query: 369 G----EVGVLHGAMSYLLQDDDGQIIEPHSISA-GLDYPGVGPEHSYLKDLGRAEYHSVT 428
           G    + G   G    +     G    P  I A GL Y G+ P  S++ + G  E  S+ 
Sbjct: 372 GVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVYEQGFMEAISIP 431

Query: 429 DDEALEAFKRLSRLEGIIPALETSHALAYLEK---LCPTLPDGTKVVLNCSGRGDKDVQT 472
             E  +   + +R EGIIPA E +HA+A   +    C    +   +++   G G  D+ +
Sbjct: 432 QIECFQGAIQFARTEGIIPAPEPTHAIAATIREALRCKETGEAKVILMAMCGHGHFDLTS 482

The following BLAST results are available for this feature:
BLAST of CSPI01G32520 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P146714.8e-22383.41Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
P252691.1e-22282.30Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
O500465.0e-22081.76Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=1692... [more]
P432841.0e-20987.94Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 G... [more]
P432835.4e-20688.69Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=... [more]
BLAST of CSPI01G32520 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0M0651.8e-27399.58Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_1G660140 PE=3 SV=1[more]
A0A1S3C0163.9e-26897.67Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103494920 PE=3 SV=1[more]
A0A5D3E2251.4e-26595.45Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold588G... [more]
A0A6J1CDB21.1e-25192.37Tryptophan synthase OS=Momordica charantia OX=3673 GN=LOC111010430 PE=3 SV=1[more]
A0A6J1J2613.1e-24991.95Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111480575 PE=3 SV=1[more]
BLAST of CSPI01G32520 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004142558.13.7e-27399.58tryptophan synthase beta chain 1 [Cucumis sativus] >KGN66694.1 hypothetical prot... [more]
XP_008454527.18.1e-26897.67PREDICTED: tryptophan synthase beta chain 1 [Cucumis melo][more]
KAA0053097.12.9e-26595.45tryptophan synthase beta chain 1 [Cucumis melo var. makuwa] >TYK29942.1 tryptoph... [more]
XP_038899607.14.6e-26395.76tryptophan synthase beta chain 1 [Benincasa hispida][more]
XP_022139544.12.4e-25192.37tryptophan synthase beta chain 1 [Momordica charantia][more]
BLAST of CSPI01G32520 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G54810.13.4e-22483.41tryptophan synthase beta-subunit 1 [more]
AT4G27070.17.6e-22482.30tryptophan synthase beta-subunit 2 [more]
AT5G28237.18.9e-15664.56Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
AT5G38530.11.7e-2930.33tryptophan synthase beta type 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 128..457
e-value: 9.5E-47
score: 159.8
IPR006654Tryptophan synthase, beta chainTIGRFAMTIGR00263TIGR00263coord: 81..469
e-value: 5.6E-187
score: 619.4
IPR006654Tryptophan synthase, beta chainCDDcd06446Trp-synth_Bcoord: 98..465
e-value: 0.0
score: 690.814
IPR023026Tryptophan synthase beta chain/beta chain-likePIRSFPIRSF001413Trp_syn_betacoord: 64..472
e-value: 1.8E-164
score: 545.7
IPR023026Tryptophan synthase beta chain/beta chain-likeHAMAPMF_00133Trp_synth_betacoord: 76..470
score: 53.143215
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 128..463
e-value: 1.3E-198
score: 661.4
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 83..285
e-value: 1.3E-198
score: 661.4
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 77..470
NoneNo IPR availablePIRSRPIRSR006278-2PIRSR006278-2coord: 151..337
e-value: 3.8E-9
score: 34.3
NoneNo IPR availablePIRSRPIRSR605856-51PIRSR605856-51coord: 147..338
e-value: 3.4E-6
score: 24.3
NoneNo IPR availablePANTHERPTHR48077TRYPTOPHAN SYNTHASE-RELATEDcoord: 23..472
NoneNo IPR availablePANTHERPTHR48077:SF8TRYPTOPHAN SYNTHASE BETA CHAIN 1, CHLOROPLASTIC-RELATEDcoord: 23..472
IPR006653Tryptophan synthase, beta chain, conserved sitePROSITEPS00168TRP_SYNTHASE_BETAcoord: 160..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G32520.1CSPI01G32520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006568 tryptophan metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004834 tryptophan synthase activity