Homology
BLAST of CSPI01G31710 vs. ExPASy Swiss-Prot
Match:
F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)
HSP 1 Score: 729.9 bits (1883), Expect = 1.8e-209
Identity = 342/480 (71.25%), Postives = 410/480 (85.42%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
ME+F +SLL++ +S+ L +LF+ H+S F+ PN PPG IG P++GES+EFLS+GWKGH
Sbjct: 1 MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDR++KY S+VFKTS++G PAA+F G NKFLFSNENKLV WWP SV+K+FPS
Sbjct: 61 PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
+TQTS+KEEAKK++KLLPQF KPEALQRYIGIMD +A+RHF W NR+EV+VFPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TF +ACRLFMS+ED HV + PF +A+G+I++PIDLPGTPF+RAIKAS FIRKE+ A
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRA 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
I++QRK DLAEGKA QDILSHMLL DE+G +MNE +I DKILGLLIGGHDTAS A T
Sbjct: 241 IIKQRKIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAIT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
F++K++AELPHIY+ VY EQMEIA +KA GE L W+D++KM+YSWNVACEV+R+A PLQG
Sbjct: 301 FLIKYMAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360
Query: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
AFREA++DFVFNGF IPKGWKLYWSANSTHK+PE FP+P FDP RFEG+GP PYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSPECFPQPENFDPTRFEGDGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
GGGPRMCPGKEYA+LEILVFMHN+VKRFKW KLL +E IIV+PMP+P KGLPVRL P +P
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNVVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPRKP 480
BLAST of CSPI01G31710 vs. ExPASy Swiss-Prot
Match:
A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)
HSP 1 Score: 728.0 bits (1878), Expect = 6.9e-209
Identity = 343/480 (71.46%), Postives = 409/480 (85.21%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
ME+F +SLL++ +S+ L++L + H+S F+ PN PPG IG P++GES+EFLS+GWKGH
Sbjct: 1 MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDR++KY S+VFKTS++G PAA+F G NKFLFSNENKLV WWP SV+K+FPS
Sbjct: 61 PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
+TQTS+KEEAKK++KLLPQFLKPEALQRY GIMD +A+RHF W NR+EV+VFPLAK F
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAKRF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TF +ACRLFMS+ED HV + PF +A+G+I++PIDLPGTPF+RAIKAS FIRKE+ A
Sbjct: 181 TFWLACRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRA 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
I++QRK DLAE KA TQDILSHMLL DE+G +MNE I DKILGLLIGGHDTAS A T
Sbjct: 241 IIKQRKIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAIT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
F+VK++AELPHIY+ VY EQMEIA +KA GE L W+D++KM+YSWNVACEV+R+A PLQG
Sbjct: 301 FLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQG 360
Query: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
AFREA++DFVFNGF IPKGWKLYWSANSTHK+ E FP+P KFDP RFEG GP PYTFVPF
Sbjct: 361 AFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRFEGAGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
GGGPRMCPGKEYA+LEIL+FMHNLVKRFKW KLL +E IIV+PMP+P KGLPVRL PH+P
Sbjct: 421 GGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPMPAKGLPVRLHPHKP 480
BLAST of CSPI01G31710 vs. ExPASy Swiss-Prot
Match:
A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)
HSP 1 Score: 716.5 bits (1848), Expect = 2.1e-205
Identity = 342/480 (71.25%), Postives = 402/480 (83.75%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKS-LFSYPNTPPGAIGLPILGESVEFLSSGWKG 60
M+LF + LL L F+SL+ + LF+ KS S PPG G P++GES EFLS+GWKG
Sbjct: 1 MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60
Query: 61 HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFP 120
+PEKFIFDRL KY S+VFKTS+ G PAA FCG CNKFLFSNENKLV WWPDSVNK+FP
Sbjct: 61 YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120
Query: 121 STTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS 180
S+TQTS+KEEA K++KLLP FLKPEALQRY+G+MD++A +HF S W+N++EVLVFPLAK+
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180
Query: 181 FTFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVV 240
+TF IAC++F+SVE+ V +L PF IA+GIIS+PIDLPGTPFN AIK+SK +R +++
Sbjct: 181 YTFWIACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLM 240
Query: 241 AIVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVAC 300
I++QRK DL EGKA TQDILSHMLLT DENG +M E DI DKILGLLIGGHDTAS AC
Sbjct: 241 GIIKQRKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSAC 300
Query: 301 TFIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQ 360
TF+VKFLAELP IY+ VY EQMEIA++K GE L WEDI+KMKYSWNVACEVLR+A PLQ
Sbjct: 301 TFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQ 360
Query: 361 GAFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVP 420
GAFREAL+DF +NGF IPKGWKLYWSANSTH+N E FPEP KFDP RFEG GP PY+FVP
Sbjct: 361 GAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEGAGPPPYSFVP 420
Query: 421 FGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQ 480
FGGGPRMCPGKEYA+LEILVFMH++VKRFKW K++ +E I+VNPMPIP KGLPVRLFPH+
Sbjct: 421 FGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480
BLAST of CSPI01G31710 vs. ExPASy Swiss-Prot
Match:
Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)
HSP 1 Score: 715.3 bits (1845), Expect = 4.6e-205
Identity = 339/477 (71.07%), Postives = 400/477 (83.86%), Query Frame = 0
Query: 4 FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHPEK 63
F +SLL+L F+SL+LF +F+ KS N PPG +G PI+GES+EFLS+GWKGHPEK
Sbjct: 5 FYLSLLLLFVTFISLSLFFIFYKQKSPL---NLPPGKMGYPIIGESLEFLSTGWKGHPEK 64
Query: 64 FIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTT- 123
FIFDR+ KY S++FKTSIVG + CG NKFLFSNENKLVT WWPDSVNKIFP+T+
Sbjct: 65 FIFDRMRKYSSELFKTSIVGESTVVCCGAASNKFLFSNENKLVTAWWPDSVNKIFPTTSL 124
Query: 124 QTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSFTF 183
++ KEE+ K++KLLPQF KPEALQRY+G+MD +A+RHF + W N+ E+ V+PLAK +TF
Sbjct: 125 DSNLKEESIKMRKLLPQFFKPEALQRYVGVMDVIAQRHFVTHWDNKNEITVYPLAKRYTF 184
Query: 184 SIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIV 243
+ACRLFMSVEDE HV + S PF+ IAAGIIS+PIDLPGTPFN+AIKAS FIRKE++ I+
Sbjct: 185 LLACRLFMSVEDENHVAKFSDPFQLIAAGIISLPIDLPGTPFNKAIKASNFIRKELIKII 244
Query: 244 RQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFI 303
+QR+ DLAEG A TQDILSHMLLT DENG MNE +I DKILGLLIGGHDTASVACTF+
Sbjct: 245 KQRRVDLAEGTASPTQDILSHMLLTSDENGKSMNELNIADKILGLLIGGHDTASVACTFL 304
Query: 304 VKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAF 363
VK+L ELPHIYD VY EQMEIA++K GE L W+D+KKMKYSWNVACEV+R++ PLQG F
Sbjct: 305 VKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNVACEVMRLSPPLQGGF 364
Query: 364 REALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPFGG 423
REA++DF+FNGF IPKGWKLYWSANSTHKN E FP P KFDP RFEGNGP PYTFVPFGG
Sbjct: 365 REAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRFEGNGPAPYTFVPFGG 424
Query: 424 GPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQ 480
GPRMCPGKEYA+LEILVFMHNLVKRFKW K++ +E IIV+P PIP K LP+RL+PH+
Sbjct: 425 GPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPIPAKDLPIRLYPHK 478
BLAST of CSPI01G31710 vs. ExPASy Swiss-Prot
Match:
I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)
HSP 1 Score: 713.4 bits (1840), Expect = 1.8e-204
Identity = 343/481 (71.31%), Postives = 399/481 (82.95%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHK--SLFSYPNTPPGAIGLPILGESVEFLSSGWK 60
MELF + LL L F+SL+ LF+ K S +P PPG G PI+GES EFLS+GWK
Sbjct: 1 MELFYVPLLSLFVLFISLSFHFLFYKSKPSSSGGFP-LPPGKTGWPIIGESYEFLSTGWK 60
Query: 61 GHPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIF 120
G+PEKFIFDR+ KY S+VFKTSI G PAA+FCG CNKFLFSNENKLV WWPDSVNK+F
Sbjct: 61 GYPEKFIFDRMTKYSSNVFKTSIFGEPAAVFCGAACNKFLFSNENKLVQAWWPDSVNKVF 120
Query: 121 PSTTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAK 180
PS+TQTS+KEEA K++K+LP F KPEALQRYIG+MD++A HF S W+N+ EV+VFPLAK
Sbjct: 121 PSSTQTSSKEEAIKMRKMLPNFFKPEALQRYIGLMDQIAANHFESGWENKNEVVVFPLAK 180
Query: 181 SFTFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEV 240
S+TF IAC++F+SVE+ V L PF IA+GIIS+PIDLPGTPFN AIK+SK +R+++
Sbjct: 181 SYTFWIACKVFVSVEEPAQVAELLEPFSAIASGIISVPIDLPGTPFNSAIKSSKIVRRKL 240
Query: 241 VAIVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVA 300
V I++QRK DL EGKA ATQDILSHMLLT DE+G +M E DI DKILGLLIGGHDTAS A
Sbjct: 241 VGIIKQRKIDLGEGKASATQDILSHMLLTSDESGKFMGEGDIADKILGLLIGGHDTASSA 300
Query: 301 CTFIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPL 360
CTF+VKFLAELP IY+ VY EQMEI ++K GE LKWEDI+KMKYSWNVACEVLR+A PL
Sbjct: 301 CTFVVKFLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNVACEVLRLAPPL 360
Query: 361 QGAFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFV 420
QGAFREALSDF +NGF IPKGWKLYWSANSTH N E FPEP KFDP RF+G GP P++FV
Sbjct: 361 QGAFREALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRFDGAGPPPFSFV 420
Query: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPH 480
PFGGGPRMCPGKEYA+LEILVFMH+LVKRFKW K++ +E I+VNPMPIP GLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPIPANGLPVRLFPH 480
BLAST of CSPI01G31710 vs. ExPASy TrEMBL
Match:
A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)
HSP 1 Score: 984.9 bits (2545), Expect = 1.2e-283
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH
Sbjct: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
Query: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of CSPI01G31710 vs. ExPASy TrEMBL
Match:
A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)
HSP 1 Score: 974.2 bits (2517), Expect = 2.0e-280
Identity = 475/483 (98.34%), Postives = 482/483 (99.79%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
ME FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGES+EFLSSGW+GH
Sbjct: 1 MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
TTQTS+KEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TFS+ACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLR+ASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
AFREAL+DFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKF+PGRFEGNGPLPYTFVPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of CSPI01G31710 vs. ExPASy TrEMBL
Match:
A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)
HSP 1 Score: 842.4 bits (2175), Expect = 9.3e-241
Identity = 407/485 (83.92%), Postives = 446/485 (91.96%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
ME FLISLLIL F+SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GES+EFLS+GWKG
Sbjct: 1 MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP+CNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
+T+ ++KE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S W+NRE VLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TFS+ACRLF+S+ED IHVERLS PF++IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
IVRQRK DLAEGKA TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDTAS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEI--ARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPL 360
FIVK+LAELP IYD VY EQMEI A+AKA+GE LKWEDI+KMKYSWNVACEVLR+ASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360
Query: 361 QGAFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFV 420
QGAFREA++DFVFNGF IPKGWKLYWS+NSTHKN YFPEPYKFDP RFEGNGP PYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420
Query: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPH 480
PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLE E IIV+P+PIP+KGLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480
Query: 481 QPLSL 484
+ LSL
Sbjct: 481 KTLSL 485
BLAST of CSPI01G31710 vs. ExPASy TrEMBL
Match:
A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)
HSP 1 Score: 834.3 bits (2154), Expect = 2.5e-238
Identity = 399/480 (83.12%), Postives = 438/480 (91.25%), Query Frame = 0
Query: 4 FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHPEK 63
FLISLLIL +SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GES+EFLS+GWKG PEK
Sbjct: 4 FLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEK 63
Query: 64 FIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQ 123
FIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFP++T+
Sbjct: 64 FIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPASTE 123
Query: 124 TSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSFTFS 183
++KE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S W+NRE+VLVFPLAKSFTFS
Sbjct: 124 INSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTFS 183
Query: 184 IACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVR 243
+ACRLF+S+ED IHVERLS PF+ IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++IVR
Sbjct: 184 LACRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVR 243
Query: 244 QRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIV 303
QRK DLAEGKA TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDT S ACTFIV
Sbjct: 244 QRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIV 303
Query: 304 KFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFR 363
+LAELP IYD VY EQMEIA+AKA GE LKWEDI+KMKYSWNVACEVLR+ASPLQGAFR
Sbjct: 304 NYLAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFR 363
Query: 364 EALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPFGGG 423
EA++ FVFNGF+IPKGWK+YWS+NSTHKN YFPEPYKFDP RFEGNGP PYTFVPFGGG
Sbjct: 364 EAINHFVFNGFYIPKGWKVYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFGGG 423
Query: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQPLSL 483
PRMCPGKEYAKLEILVFMHNLVKRFKWTKLL+ E IIV+P+PIP+KGLPVRLFPH LSL
Sbjct: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLDKEKIIVDPLPIPEKGLPVRLFPHTTLSL 483
BLAST of CSPI01G31710 vs. ExPASy TrEMBL
Match:
A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)
HSP 1 Score: 829.7 bits (2142), Expect = 6.2e-237
Identity = 394/482 (81.74%), Postives = 432/482 (89.63%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
ME FL+SLLI + + LFILF+ HKS FSYPN PPG IG PI+GES+EF S+GWKG
Sbjct: 1 MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDR+NKYKS +FKTSIVG PAA+FCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
+TQTS+ EEAKKLKKLLPQFLKPEAL RYIGIMD LA RHF S W+N+++VLVFPLAKSF
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKSF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TFS+ACRLF+SVEDE HVERLSGPF+HIAAGIISMPID PGTPFNR IKAS FIRKE+++
Sbjct: 181 TFSLACRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLS 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
IV+QRK DLAEGKA TQDILSHMLLTCDENG YMNE DI DKILGLLIGGHDTAS ACT
Sbjct: 241 IVKQRKVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
FIVKFLAELPHIYD VY EQM IA+AKA GE L WEDI+KMKYSWNVACEVLR+ASPLQG
Sbjct: 301 FIVKFLAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
AFREA++DFVFNGF+IPKGWKLYWS+NSTHKN +YFP+PY FDP RFEGNGP PYTFVPF
Sbjct: 361 AFREAINDFVFNGFYIPKGWKLYWSSNSTHKNADYFPDPYTFDPSRFEGNGPAPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
GGGPRMCPGKEYA+LEILVFMHNLVKRFKW K+LE E I+V+PMPIPQKGLPVRLFPH+
Sbjct: 421 GGGPRMCPGKEYARLEILVFMHNLVKRFKWAKILEKEKIVVDPMPIPQKGLPVRLFPHKT 480
Query: 481 LS 483
+S
Sbjct: 481 VS 482
BLAST of CSPI01G31710 vs. NCBI nr
Match:
XP_004139087.1 (beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein Csa_007564 [Cucumis sativus])
HSP 1 Score: 984.9 bits (2545), Expect = 2.4e-283
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH
Sbjct: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA
Sbjct: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
Query: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF
Sbjct: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of CSPI01G31710 vs. NCBI nr
Match:
XP_008443638.1 (PREDICTED: beta-amyrin 28-oxidase [Cucumis melo])
HSP 1 Score: 974.2 bits (2517), Expect = 4.2e-280
Identity = 475/483 (98.34%), Postives = 482/483 (99.79%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
ME FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGES+EFLSSGW+GH
Sbjct: 1 MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
TTQTS+KEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TFS+ACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA
Sbjct: 181 TFSLACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT
Sbjct: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLR+ASPLQG
Sbjct: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQG 360
Query: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
AFREAL+DFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKF+PGRFEGNGPLPYTFVPF
Sbjct: 361 AFREALNDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFEPGRFEGNGPLPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of CSPI01G31710 vs. NCBI nr
Match:
XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])
HSP 1 Score: 902.9 bits (2332), Expect = 1.2e-258
Identity = 435/483 (90.06%), Postives = 460/483 (95.24%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
ME FLISLLI FFLSLTLFILFHNHKSLFSYPN PPGAIGLPILGES+EFLS+GWKG
Sbjct: 1 MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDRL+KYKSDVFKTSIVGVPAAIFCG CNKFLFSNENKLVTPWWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
+TQTS+KEEAKKLKK LPQFLKPEALQRYIG+MDELA+RHF SFW+NREEVLVFPLAKSF
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKSF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TFS+ACRLF+SVEDEIHV+RLSGPFEHIAAGIIS+PIDLPGTPFNRAIKASKFIRKE+VA
Sbjct: 181 TFSLACRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVA 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
IV++R+ DLAEGKA ATQDILSHMLLTCDENGV+MNESDI DKILGLLIGGHDTASVACT
Sbjct: 241 IVKKRRWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQG 360
FIVKFLAELPHIY VYTEQMEIA+ KA GE LKWEDIKKMKYSWNV CEVLR+ASP QG
Sbjct: 301 FIVKFLAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQG 360
Query: 361 AFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPF 420
AFREAL+DFVFNGF+IPKGWKLYWSANSTHK+PEYFPEPYKF+P RFEGNGPLPYTFVPF
Sbjct: 361 AFREALTDFVFNGFYIPKGWKLYWSANSTHKSPEYFPEPYKFEPARFEGNGPLPYTFVPF 420
Query: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQP 480
GGGPRMCPGKEYAKLEILVFMHNLVKRFKW KLLE ENIIV+PMPIPQKGLP+RLFPHQP
Sbjct: 421 GGGPRMCPGKEYAKLEILVFMHNLVKRFKWAKLLEKENIIVDPMPIPQKGLPIRLFPHQP 480
Query: 481 LSL 484
LSL
Sbjct: 481 LSL 483
BLAST of CSPI01G31710 vs. NCBI nr
Match:
XP_022987538.1 (beta-amyrin 28-oxidase [Cucurbita maxima])
HSP 1 Score: 842.4 bits (2175), Expect = 1.9e-240
Identity = 407/485 (83.92%), Postives = 446/485 (91.96%), Query Frame = 0
Query: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
ME FLISLLIL F+SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GES+EFLS+GWKG
Sbjct: 1 MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP+CNKFLFSNENKLVT WWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSF 180
+T+ ++KE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S W+NRE VLVFPLAKSF
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKSF 180
Query: 181 TFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVA 240
TFS+ACRLF+S+ED IHVERLS PF++IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++
Sbjct: 181 TFSLACRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLS 240
Query: 241 IVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACT 300
IVRQRK DLAEGKA TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDTAS ACT
Sbjct: 241 IVRQRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACT 300
Query: 301 FIVKFLAELPHIYDAVYTEQMEI--ARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPL 360
FIVK+LAELP IYD VY EQMEI A+AKA+GE LKWEDI+KMKYSWNVACEVLR+ASPL
Sbjct: 301 FIVKYLAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPL 360
Query: 361 QGAFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFV 420
QGAFREA++DFVFNGF IPKGWKLYWS+NSTHKN YFPEPYKFDP RFEGNGP PYTFV
Sbjct: 361 QGAFREAINDFVFNGFNIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFV 420
Query: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPH 480
PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLE E IIV+P+PIP+KGLPVRLFPH
Sbjct: 421 PFGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLEKEKIIVDPLPIPEKGLPVRLFPH 480
Query: 481 QPLSL 484
+ LSL
Sbjct: 481 KTLSL 485
BLAST of CSPI01G31710 vs. NCBI nr
Match:
XP_023516848.1 (beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 839.3 bits (2167), Expect = 1.6e-239
Identity = 400/480 (83.33%), Postives = 442/480 (92.08%), Query Frame = 0
Query: 4 FLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHPEK 63
FLISLLIL F+SLTLF+LF+ HKSLFSYPNTPPGAIGLPI+GES+EFLS+GWKG PEK
Sbjct: 4 FLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGEPEK 63
Query: 64 FIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQ 123
FIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFP++T+
Sbjct: 64 FIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPASTE 123
Query: 124 TSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSFTFS 183
++KE A +LKKLLP+FLKPEALQRYIG+MDELA+RHF S W+NRE+VLVFPLAKSFTFS
Sbjct: 124 INSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKSFTFS 183
Query: 184 IACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVR 243
+ACRLF+S+ED IHVERLS PF++IAAGIISMPIDLPGTPFNRAIKAS F+RKE+++IVR
Sbjct: 184 LACRLFVSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVR 243
Query: 244 QRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIV 303
QRK DLAEGKA TQDILSHMLLTCDE G YMNES I D ILGLLIGGHDT S ACTFIV
Sbjct: 244 QRKSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIV 303
Query: 304 KFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFR 363
K+LAELP IYD+VY EQMEIA+AK GE LKWEDI+KMKYSWNVACEVLR+ASPLQGAFR
Sbjct: 304 KYLAELPQIYDSVYREQMEIAKAKGAGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFR 363
Query: 364 EALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPFGGG 423
E ++DFVFNGF+IPKGWKLYWS+NSTHKN YFPEPYKFDP RFEGNGP PYTFVPFGGG
Sbjct: 364 EPINDFVFNGFYIPKGWKLYWSSNSTHKNHAYFPEPYKFDPSRFEGNGPAPYTFVPFGGG 423
Query: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFPHQPLSL 483
PRMCPGKEYAKLEILVFMHNLVKRFKWTKL++ E IIV+P+PIP+KGLPVRLFPH+ LSL
Sbjct: 424 PRMCPGKEYAKLEILVFMHNLVKRFKWTKLMDKEKIIVDPLPIPEKGLPVRLFPHKYLSL 483
BLAST of CSPI01G31710 vs. TAIR 10
Match:
AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )
HSP 1 Score: 634.8 bits (1636), Expect = 5.7e-182
Identity = 300/479 (62.63%), Postives = 371/479 (77.45%), Query Frame = 0
Query: 3 LFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHPE 62
+++ ++IL + L+L +L H S FSYPN PPG GLP++GES FLS+G +GHPE
Sbjct: 1 MYMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPE 60
Query: 63 KFIFDRLNKYKSD---VFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFP 122
KFI DR+ ++ S VFKT + G P A+ G NKFLF+NENKLV WWPDSVNKIFP
Sbjct: 61 KFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFP 120
Query: 123 STTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS 182
S+ QTS+KEEA+KL+ LL QF+KPEAL+RY+G+MDE+A+RHF + W N+++V+VFPL K
Sbjct: 121 SSMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKK 180
Query: 183 FTFSIACRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVV 242
FTFSIACR F+S+ED V +L F +A GI S+PIDLPGT FNRAIKAS+ +RKEV
Sbjct: 181 FTFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVS 240
Query: 243 AIVRQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVAC 302
AIVRQRK++L GKAL DILSHML+ E + D+ DKI+GLLIGGHDTAS+ C
Sbjct: 241 AIVRQRKEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVC 300
Query: 303 TFIVKFLAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQ 362
TF+V +LAE PH+Y V EQ EI + K E E L+WEDI+KM+YSWNVACEV+RI PL
Sbjct: 301 TFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLS 360
Query: 363 GAFREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVP 422
G FREA+ F F GF+IPKGWKLYWSA +TH NP+YFPEP +F+P RFEG+GP PYT+VP
Sbjct: 361 GTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVP 420
Query: 423 FGGGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENEN-IIVNPMPIPQKGLPVRLFP 478
FGGGPRMCPGKEYA+LEIL+FMHNLV RFKW K+ NEN I+V+P+PIP KGLP+R+FP
Sbjct: 421 FGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFP 475
BLAST of CSPI01G31710 vs. TAIR 10
Match:
AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )
HSP 1 Score: 359.0 bits (920), Expect = 6.0e-99
Identity = 177/318 (55.66%), Postives = 231/318 (72.64%), Query Frame = 0
Query: 9 LILLFFFLSLTLF-ILFH--NHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHPEKFI 68
L ++F F+S +F +LF H S F YPN PPG IG P++GE++ FLS+G +GHPEKF+
Sbjct: 3 LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62
Query: 69 FDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPSTTQTS 128
DR+ + S +FKT + G P A+ G NKFLF+NENKLV WWPDSVNKIFPS+TQTS
Sbjct: 63 TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122
Query: 129 TKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSFTFSIA 188
+KEEA K + LL +KPEAL+RY+G+MDE+A++HF + W N+++++VFPL K FTFSIA
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTKKFTFSIA 182
Query: 189 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 248
CRLF+S++D V +L PF + G+ S+PIDLPGT FNRAIKAS+ + KEV I+RQR
Sbjct: 183 CRLFLSMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQR 242
Query: 249 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 308
K++L GK QDILSHML+ E + D+ DKI+ LLIGGHDT S+ CTF+V +
Sbjct: 243 KEELKAGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVVNY 302
Query: 309 LAELPHIYDAVYTEQMEI 324
LAE PHIY V E M+I
Sbjct: 303 LAEFPHIYQRV-LEGMQI 315
BLAST of CSPI01G31710 vs. TAIR 10
Match:
AT2G42850.1 (cytochrome P450, family 718 )
HSP 1 Score: 278.9 bits (712), Expect = 7.9e-75
Identity = 158/470 (33.62%), Postives = 256/470 (54.47%), Query Frame = 0
Query: 16 LSLTLFILFHNH-----KSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHP-EKFIFDRL 75
+S TLF H K + PG +GLP +GE+++F + E F+ R+
Sbjct: 20 ISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRI 79
Query: 76 NKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWP-DSVNKIFPSTTQTSTKE 135
K+ ++FKT I+G P + G N+ + SNE LV WP SV + + E
Sbjct: 80 IKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGE 139
Query: 136 EAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSFTFSIACRL 195
+ + L+ ++ L L+ I + + + H + W+ +EE+ ++ AK TF++
Sbjct: 140 KHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFEC 199
Query: 196 FMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQRKQD 255
++ EI + + FE + G+ ++P++ P + F RA KA I +V VR+++++
Sbjct: 200 LYGIKVEIGMLEV---FERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRRE 259
Query: 256 LAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKFLAE 315
+ + A L L+ GV + E ++ D ++ L+ HDT S A + K LA+
Sbjct: 260 MEKEGAEKPNTTLFSRLVEELIKGV-ITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQ 319
Query: 316 LPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFREALSD 375
P D + E +I K EGE L ED+KKMKYSW V E +R++ P+ G+FR+A++D
Sbjct: 320 HPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVAD 379
Query: 376 FVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPL-PYTFVPFGGGPRMC 435
+ G+ IPKGWK+ W+ TH NPE F +P FDP RF+ P+ YT++PFGGGPR+C
Sbjct: 380 IDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFD--KPIQAYTYLPFGGGPRLC 439
Query: 436 PGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFP 478
G + AK+ ILVFMH +V F W+ + +E I ++P+P P G+P+++ P
Sbjct: 440 AGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISP 482
BLAST of CSPI01G31710 vs. TAIR 10
Match:
AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )
HSP 1 Score: 257.3 bits (656), Expect = 2.5e-68
Identity = 154/462 (33.33%), Postives = 248/462 (53.68%), Query Frame = 0
Query: 15 FLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHPEKFIFDRLNKYKS 74
FL L FI S P PPG +G P +GE+ + S P F + +Y S
Sbjct: 16 FLCLLRFIAGVRRSSSTKLP-LPPGTMGYPYVGETFQLYSQ----DPNVFFAAKQRRYGS 75
Query: 75 DVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIF-PSTTQTSTKEEAKKL 134
VFKT ++G P + P KF+ ++ L P +P S ++ + KL
Sbjct: 76 -VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKL 135
Query: 135 KKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSFTFSIACRLFMSVE 194
+KL+ + P+A++ + ++ +A+ NS+ + ++ + K++TF++A + +
Sbjct: 136 RKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVALISILGKD 195
Query: 195 DEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQRKQDLAEGK 254
+ + E L + + G SMPI+LPGT F++A+KA K + + + I+ +R+Q+ +
Sbjct: 196 EVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPS--- 255
Query: 255 ALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKFLAELPHIY 314
+ D+L + E+ + + I D I+G++ DT + T+I+K+LA+ P +
Sbjct: 256 --SHTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVL 315
Query: 315 DAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFREALSDFVFNG 374
+AV EQM I + K EGE+L WED KKM ++ V E LR A+ L FREA+ D + G
Sbjct: 316 EAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEG 375
Query: 375 FFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPFGGGPRMCPGKEYA 434
+ IPKGWK+ + H N + F +P KFDP RFE P P TF+PFG G CPG E A
Sbjct: 376 YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE-VAPKPNTFMPFGSGIHSCPGNELA 435
Query: 435 KLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRL 476
KLEI V +H+L +++W+ + ++ I P +PQ GLP+ L
Sbjct: 436 KLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459
BLAST of CSPI01G31710 vs. TAIR 10
Match:
AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )
HSP 1 Score: 252.3 bits (643), Expect = 7.9e-67
Identity = 153/476 (32.14%), Postives = 251/476 (52.73%), Query Frame = 0
Query: 5 LISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGHPEKF 64
LI+L I++ + L + L + L PPG++GLP +GE++ + +P F
Sbjct: 22 LITLTIVVVVVVLLFKWWLHWKEQRL----RLPPGSMGLPYIGETLRLYTE----NPNSF 81
Query: 65 IFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNK-IFPSTTQ 124
R NKY D+FKT I+G P + P + + ++ L P +P S + I P
Sbjct: 82 FATRQNKY-GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALF 141
Query: 125 TSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKSFTFS 184
LK+L+ P AL+ + ++ L + +S W +++ + K + F
Sbjct: 142 FHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSS-WTSQKSINTLEYMKRYAFD 201
Query: 185 IA-CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIV 244
+A F E+ ++ + ++ + G SMP+DLPGT F++++KA + +E+ ++
Sbjct: 202 VAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI 261
Query: 245 RQRKQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFI 304
+R+++ E L L +L D+ +++S I D I+G++ DT + T++
Sbjct: 262 EKRRENGREEGGL-----LGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWL 321
Query: 305 VKFLAELPHIYDAVYTEQMEI-ARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGA 364
+K+L + P++ V EQ I + K E + WED +KM + V E LR AS L
Sbjct: 322 LKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFT 381
Query: 365 FREALSDFVFNGFFIPKGWKLYWSANSTHKNPEYFPEPYKFDPGRFEGNGPLPYTFVPFG 424
FREA+ D ++G+ IPKGWK+ H + E+FP+P KFDP RFE P PYT++PFG
Sbjct: 382 FREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFG 441
Query: 425 GGPRMCPGKEYAKLEILVFMHNLVKRFKWTKLLENENIIVNPMPIPQKGLPVRLFP 478
G CPG E AKLE+L+ +H+L F+W + + E I P P+P+KGLP+R+ P
Sbjct: 442 NGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F6H9N6 | 1.8e-209 | 71.25 | Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1 | [more] |
A5BFI4 | 6.9e-209 | 71.46 | Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2 | [more] |
A0A0S2II38 | 2.1e-205 | 71.25 | Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... | [more] |
Q2MJ20 | 4.6e-205 | 71.07 | Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV... | [more] |
I7C6E8 | 1.8e-204 | 71.31 | Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LXN3 | 1.2e-283 | 100.00 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1 | [more] |
A0A1S3B8H4 | 2.0e-280 | 98.34 | beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1 | [more] |
A0A6J1JJQ7 | 9.3e-241 | 83.92 | beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1 | [more] |
A0A6J1H8T4 | 2.5e-238 | 83.13 | beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1 | [more] |
A0A6J1DZX7 | 6.2e-237 | 81.74 | beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_004139087.1 | 2.4e-283 | 100.00 | beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein ... | [more] |
XP_008443638.1 | 4.2e-280 | 98.34 | PREDICTED: beta-amyrin 28-oxidase [Cucumis melo] | [more] |
XP_038877759.1 | 1.2e-258 | 90.06 | LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida] | [more] |
XP_022987538.1 | 1.9e-240 | 83.92 | beta-amyrin 28-oxidase [Cucurbita maxima] | [more] |
XP_023516848.1 | 1.6e-239 | 83.33 | beta-amyrin 28-oxidase [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
AT5G36110.1 | 5.7e-182 | 62.63 | cytochrome P450, family 716, subfamily A, polypeptide 1 | [more] |
AT5G36140.1 | 6.0e-99 | 55.66 | cytochrome P450, family 716, subfamily A, polypeptide 2 | [more] |
AT2G42850.1 | 7.9e-75 | 33.62 | cytochrome P450, family 718 | [more] |
AT5G45340.1 | 2.5e-68 | 33.33 | cytochrome P450, family 707, subfamily A, polypeptide 3 | [more] |
AT2G29090.1 | 7.9e-67 | 32.14 | cytochrome P450, family 707, subfamily A, polypeptide 2 | [more] |