CSPI01G31600 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G31600
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDUF4220 domain-containing protein
LocationChr1: 26266726 .. 26268884 (+)
RNA-Seq ExpressionCSPI01G31600
SyntenyCSPI01G31600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATAGATAGAAACAAAGAAAGACATCCTTGTTTCCCTTTCTCATAGCCTAAATCAAGTAGAGATTGGATTAGATTAGATCATCTTCATCTTGTTGGATCCCATGGAAATATTTCCACCTTCTTTTTGGGAATTCATGTACCTATGGGAACCTTCAGCTGCTCTGTTTCTAAGTTTATGTGTGCAGCTGGTTCTTGTTCCTTTGGGGTTAAAAAGAAAATGCAGCTCAAGCCAATTCCTTCGTTTCTTTCTCTTAATTGCATACACCTTTTCTGATTGGATTGCCAATTTCTCATTCGTCATGCTTGTCGAAAGATATGGTACCGGCTGCTATGACGATTTCACCGACCCCATGTACATGATTAGAGCATTCTTAGCTCATTTTTTACTACTTCACTTAGGTGGCTCTGACACTATCACTGCCTATTCAATGGAAGACAATGAATTATGGCTTAGAACCCTTCTTTCCATGCTTGCCATTCTTGCTGCTTCAATTTACATCTTCTTGCAAGCCCTGCTACCAACTTCTCTGAACTATATTTCCATTCCTGTTATTATTGCTGGGGTAATCAAGAATAGTGAGAAGATATGGGCTTTGAGATCCGCTTCCGCTGAACGCTTGCGAGATTTTCTCGCCGTTTCAACTCCTTCTCCAATCACTACCCATAATGAAGAGGAGGTGCAAGATTTTGAGGTGCTACGCATTGCTTACTACTTTTTCATCAGAGACAAACGGTTGTTTGTAGGTTTGGGTCCGACCTCTTATGATCTTCAACAAAATCGCCTTTCCTATTACGAAAAATTCGAATCTAAATCTGCTTTCAAAATCATTGAGCTTGAGCTTGGATTCATGTATGATTTCTTCTACACAAAAGCCTCCATCAATCATTCTCTTTGTGGCCGTCTTTTTCGCCTCACTACCTTTTCTTCTCTTGTCATCGCCTTTCTCACCTATTGCCTGATTGATAAGCAGGAATATCCTTCAACCTATGTGAATCTCATATTTCTCCTCTTCTCTGGAGCTTTGAGTATTGAAATCTATTCACTTTTTTTATTTCTATTCTCTGATTGGAATGTAATCTGGTTATTGACCACTCAATCCCCTTCCAATCCCCTGCCTCGTCTTGCTTTAAAACTAATCTCTCTTTGTGGATGGTCTCTCAAGAAAAGAAGATGCTCCAATTCTATTTCTCAGTACAATCTAATCTCTCATTGTTTGGAACAGAAAAATGATAGCTATTATTTCAAATTCCCCAGCACCAAAACCATAGCAGCATTTTCAGTACAGCGTCCCATCTCTAATAACCTTGAAGCACATATCTTCCAACAACTAAAGCAAAAGTTGGTACTTAACCAAGAGTATGATTATGGTTATAATGAAATTGGTTGGAGCCTAAAATTAGATTTGGACCAATCCATCCTCATCTGGCATATTGCTACCGATTTTTGCTATCATTCTTCGCCAAAATTCAAAGAGAGTGAAGAATCAAAGTCCTGTATTCCACCCCAAGATAGCGTATCGCTGTCTAACTTTTTGGCTTACTTTATAGTGCACCATCCTTCCTTATTTCCCAGTGGAATGAGTCAAATAAGGCATAAGGCTACTAGTGAACATGTGCTTGAATTATTGCAAGACGAGAAATTAGATAGGTGTAGAAGTAATATGTTGAAGAATTTGGAGTTGAAGATTGAGGTTGTTAAAGAAGAGAGAAAGGAGTCGAGGGTTTTGGATGCTTTTCGTCTTGCAGGGTTTCTTGAAAAGTTAGAACAGTCACAGAAATGGGAAATAATAGGGAATGTGTGGGTGGAATTGCTTGGACGTCTTTCATGTGAATGTGAATGGTATGATCATGCTAAACAGCTTACACAAGGAGGAAGTTTGGTAACTCGTGTCTGGATTTTGATGCATCATCTTGGATATCTCAAACAAAATGACGTTTTCACTACGCATATCCAAGAAGATCAACAAGATACAACACCACTTCTAGGCCATGAAATCGTAGCAGATTATGTGGTGGAACAGATGTTAAATGTTGTATTCAATATTTCTTCTTCCCTCTAAATTCAAATTTTAGTTAGGTAAATGAGATTACAAGGTTTTGCATATTAGTGAATTGTTAGGGATTTTGGTTGGCTTATTTTATTAAACTTAAAATTGTAG

mRNA sequence

CATAGATAGAAACAAAGAAAGACATCCTTGTTTCCCTTTCTCATAGCCTAAATCAAGTAGAGATTGGATTAGATTAGATCATCTTCATCTTGTTGGATCCCATGGAAATATTTCCACCTTCTTTTTGGGAATTCATGTACCTATGGGAACCTTCAGCTGCTCTGTTTCTAAGTTTATGTGTGCAGCTGGTTCTTGTTCCTTTGGGGTTAAAAAGAAAATGCAGCTCAAGCCAATTCCTTCGTTTCTTTCTCTTAATTGCATACACCTTTTCTGATTGGATTGCCAATTTCTCATTCGTCATGCTTGTCGAAAGATATGGTACCGGCTGCTATGACGATTTCACCGACCCCATGTACATGATTAGAGCATTCTTAGCTCATTTTTTACTACTTCACTTAGGTGGCTCTGACACTATCACTGCCTATTCAATGGAAGACAATGAATTATGGCTTAGAACCCTTCTTTCCATGCTTGCCATTCTTGCTGCTTCAATTTACATCTTCTTGCAAGCCCTGCTACCAACTTCTCTGAACTATATTTCCATTCCTGTTATTATTGCTGGGGTAATCAAGAATAGTGAGAAGATATGGGCTTTGAGATCCGCTTCCGCTGAACGCTTGCGAGATTTTCTCGCCGTTTCAACTCCTTCTCCAATCACTACCCATAATGAAGAGGAGGTGCAAGATTTTGAGGTGCTACGCATTGCTTACTACTTTTTCATCAGAGACAAACGGTTGTTTGTAGGTTTGGGTCCGACCTCTTATGATCTTCAACAAAATCGCCTTTCCTATTACGAAAAATTCGAATCTAAATCTGCTTTCAAAATCATTGAGCTTGAGCTTGGATTCATGTATGATTTCTTCTACACAAAAGCCTCCATCAATCATTCTCTTTGTGGCCGTCTTTTTCGCCTCACTACCTTTTCTTCTCTTGTCATCGCCTTTCTCACCTATTGCCTGATTGATAAGCAGGAATATCCTTCAACCTATGTGAATCTCATATTTCTCCTCTTCTCTGGAGCTTTGAGTATTGAAATCTATTCACTTTTTTTATTTCTATTCTCTGATTGGAATGTAATCTGGTTATTGACCACTCAATCCCCTTCCAATCCCCTGCCTCGTCTTGCTTTAAAACTAATCTCTCTTTGTGGATGGTCTCTCAAGAAAAGAAGATGCTCCAATTCTATTTCTCAGTACAATCTAATCTCTCATTGTTTGGAACAGAAAAATGATAGCTATTATTTCAAATTCCCCAGCACCAAAACCATAGCAGCATTTTCAGTACAGCGTCCCATCTCTAATAACCTTGAAGCACATATCTTCCAACAACTAAAGCAAAAGTTGGTACTTAACCAAGAGTATGATTATGGTTATAATGAAATTGGTTGGAGCCTAAAATTAGATTTGGACCAATCCATCCTCATCTGGCATATTGCTACCGATTTTTGCTATCATTCTTCGCCAAAATTCAAAGAGAGTGAAGAATCAAAGTCCTGTATTCCACCCCAAGATAGCGTATCGCTGTCTAACTTTTTGGCTTACTTTATAGTGCACCATCCTTCCTTATTTCCCAGTGGAATGAGTCAAATAAGGCATAAGGCTACTAGTGAACATGTGCTTGAATTATTGCAAGACGAGAAATTAGATAGGTGTAGAAGTAATATGTTGAAGAATTTGGAGTTGAAGATTGAGGTTGTTAAAGAAGAGAGAAAGGAGTCGAGGGTTTTGGATGCTTTTCGTCTTGCAGGGTTTCTTGAAAAGTTAGAACAGTCACAGAAATGGGAAATAATAGGGAATGTGTGGGTGGAATTGCTTGGACGTCTTTCATGTGAATGTGAATGGTATGATCATGCTAAACAGCTTACACAAGGAGGAAGTTTGGTAACTCGTGTCTGGATTTTGATGCATCATCTTGGATATCTCAAACAAAATGACGTTTTCACTACGCATATCCAAGAAGATCAACAAGATACAACACCACTTCTAGGCCATGAAATCGTAGCAGATTATGTGGTGGAACAGATGTTAAATGTTGTATTCAATATTTCTTCTTCCCTCTAAATTCAAATTTTAGTTAGGTAAATGAGATTACAAGGTTTTGCATATTAGTGAATTGTTAGGGATTTTGGTTGGCTTATTTTATTAAACTTAAAATTGTAG

Coding sequence (CDS)

ATGGAAATATTTCCACCTTCTTTTTGGGAATTCATGTACCTATGGGAACCTTCAGCTGCTCTGTTTCTAAGTTTATGTGTGCAGCTGGTTCTTGTTCCTTTGGGGTTAAAAAGAAAATGCAGCTCAAGCCAATTCCTTCGTTTCTTTCTCTTAATTGCATACACCTTTTCTGATTGGATTGCCAATTTCTCATTCGTCATGCTTGTCGAAAGATATGGTACCGGCTGCTATGACGATTTCACCGACCCCATGTACATGATTAGAGCATTCTTAGCTCATTTTTTACTACTTCACTTAGGTGGCTCTGACACTATCACTGCCTATTCAATGGAAGACAATGAATTATGGCTTAGAACCCTTCTTTCCATGCTTGCCATTCTTGCTGCTTCAATTTACATCTTCTTGCAAGCCCTGCTACCAACTTCTCTGAACTATATTTCCATTCCTGTTATTATTGCTGGGGTAATCAAGAATAGTGAGAAGATATGGGCTTTGAGATCCGCTTCCGCTGAACGCTTGCGAGATTTTCTCGCCGTTTCAACTCCTTCTCCAATCACTACCCATAATGAAGAGGAGGTGCAAGATTTTGAGGTGCTACGCATTGCTTACTACTTTTTCATCAGAGACAAACGGTTGTTTGTAGGTTTGGGTCCGACCTCTTATGATCTTCAACAAAATCGCCTTTCCTATTACGAAAAATTCGAATCTAAATCTGCTTTCAAAATCATTGAGCTTGAGCTTGGATTCATGTATGATTTCTTCTACACAAAAGCCTCCATCAATCATTCTCTTTGTGGCCGTCTTTTTCGCCTCACTACCTTTTCTTCTCTTGTCATCGCCTTTCTCACCTATTGCCTGATTGATAAGCAGGAATATCCTTCAACCTATGTGAATCTCATATTTCTCCTCTTCTCTGGAGCTTTGAGTATTGAAATCTATTCACTTTTTTTATTTCTATTCTCTGATTGGAATGTAATCTGGTTATTGACCACTCAATCCCCTTCCAATCCCCTGCCTCGTCTTGCTTTAAAACTAATCTCTCTTTGTGGATGGTCTCTCAAGAAAAGAAGATGCTCCAATTCTATTTCTCAGTACAATCTAATCTCTCATTGTTTGGAACAGAAAAATGATAGCTATTATTTCAAATTCCCCAGCACCAAAACCATAGCAGCATTTTCAGTACAGCGTCCCATCTCTAATAACCTTGAAGCACATATCTTCCAACAACTAAAGCAAAAGTTGGTACTTAACCAAGAGTATGATTATGGTTATAATGAAATTGGTTGGAGCCTAAAATTAGATTTGGACCAATCCATCCTCATCTGGCATATTGCTACCGATTTTTGCTATCATTCTTCGCCAAAATTCAAAGAGAGTGAAGAATCAAAGTCCTGTATTCCACCCCAAGATAGCGTATCGCTGTCTAACTTTTTGGCTTACTTTATAGTGCACCATCCTTCCTTATTTCCCAGTGGAATGAGTCAAATAAGGCATAAGGCTACTAGTGAACATGTGCTTGAATTATTGCAAGACGAGAAATTAGATAGGTGTAGAAGTAATATGTTGAAGAATTTGGAGTTGAAGATTGAGGTTGTTAAAGAAGAGAGAAAGGAGTCGAGGGTTTTGGATGCTTTTCGTCTTGCAGGGTTTCTTGAAAAGTTAGAACAGTCACAGAAATGGGAAATAATAGGGAATGTGTGGGTGGAATTGCTTGGACGTCTTTCATGTGAATGTGAATGGTATGATCATGCTAAACAGCTTACACAAGGAGGAAGTTTGGTAACTCGTGTCTGGATTTTGATGCATCATCTTGGATATCTCAAACAAAATGACGTTTTCACTACGCATATCCAAGAAGATCAACAAGATACAACACCACTTCTAGGCCATGAAATCGTAGCAGATTATGTGGTGGAACAGATGTTAAATGTTGTATTCAATATTTCTTCTTCCCTCTAA

Protein sequence

MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWIANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLLSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVSTPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSNSISQYNLISHCLEQKNDSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQEYDYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAYFIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELKIEVVKEERKESRVLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVWILMHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVFNISSSL*
Homology
BLAST of CSPI01G31600 vs. ExPASy TrEMBL
Match: A0A0A0LZZ2 (DUF4220 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G638510 PE=4 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 649/652 (99.54%), Postives = 650/652 (99.69%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI
Sbjct: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL
Sbjct: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
           LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS
Sbjct: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180

Query: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240
           TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF
Sbjct: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240

Query: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300
           KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI
Sbjct: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300

Query: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360
           FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN
Sbjct: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360

Query: 361 SISQYNLISHCLEQKNDSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQEY 420
           SISQYNLISHCLEQKNDSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQEY
Sbjct: 361 SISQYNLISHCLEQKNDSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQEY 420

Query: 421 DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAY 480
           DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAY
Sbjct: 421 DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAY 480

Query: 481 FIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELKIEVVKEERKESR 540
           FIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLEL IEVVKEERKESR
Sbjct: 481 FIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELNIEVVKEERKESR 540

Query: 541 VLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVWIL 600
           VLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGR+SCECEWYDHAKQLTQGGSLVTRVWIL
Sbjct: 541 VLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRISCECEWYDHAKQLTQGGSLVTRVWIL 600

Query: 601 MHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVFNISSSL 653
           MHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVFN SSSL
Sbjct: 601 MHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVFNNSSSL 652

BLAST of CSPI01G31600 vs. ExPASy TrEMBL
Match: A0A5D3BS41 (DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00060 PE=4 SV=1)

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 602/655 (91.91%), Postives = 626/655 (95.57%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           MEIFPPSFW+FMYLWEPSAALFLSLCVQLVLVP+GLKRK SSSQFLRFFLLIAY+FSDWI
Sbjct: 1   MEIFPPSFWDFMYLWEPSAALFLSLCVQLVLVPMGLKRKHSSSQFLRFFLLIAYSFSDWI 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           ANFSFVMLVERYGTGCYDDFTDP Y+IRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL
Sbjct: 61  ANFSFVMLVERYGTGCYDDFTDPNYIIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
           LS+LA+LAASIYIFLQALLPTSLNY+SIPVIIAG+IKN EKIWALRSASAERLRDFLAVS
Sbjct: 121 LSLLAVLAASIYIFLQALLPTSLNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVS 180

Query: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240
           TPSPITTHNEEEVQDFE+L IAYYFF RDKRLFVGLGPTSYDLQQNRLSYYEKF+S SAF
Sbjct: 181 TPSPITTHNEEEVQDFEMLHIAYYFFNRDKRLFVGLGPTSYDLQQNRLSYYEKFQSNSAF 240

Query: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300
           KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSL IA LTYCLIDKQEYPSTYVNLI
Sbjct: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAILTYCLIDKQEYPSTYVNLI 300

Query: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360
           FLLF GALSIEIYSLFL LFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWS KKRRCSN
Sbjct: 301 FLLFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSFKKRRCSN 360

Query: 361 SISQYNLISHCLEQKN-DSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQE 420
           SISQYNLISHCL+QKN DSYY KF +TKT+AAFSVQRPISNNLEAHIFQQLK+KLVLNQE
Sbjct: 361 SISQYNLISHCLKQKNDDSYYCKFHNTKTMAAFSVQRPISNNLEAHIFQQLKKKLVLNQE 420

Query: 421 YDYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEE-SKSCIPPQDSVSLSNFL 480
           YD GYNEIGWSLKLDLDQSIL+WHIATDFCY+SSPKFKESEE S+SCIPPQDS+SLSNFL
Sbjct: 421 YDSGYNEIGWSLKLDLDQSILLWHIATDFCYYSSPKFKESEEYSESCIPPQDSISLSNFL 480

Query: 481 AYFIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELKIEVVKEERKE 540
           AYFIVHHPSLFPSGMSQIRHKATSE VLELLQD+KL RC SNMLKNLELKIEVVKEERKE
Sbjct: 481 AYFIVHHPSLFPSGMSQIRHKATSEDVLELLQDKKLGRCNSNMLKNLELKIEVVKEERKE 540

Query: 541 SRVLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVW 600
           S VLDA RLAG LEKLEQSQKWEIIGNVWVELLGR+SCE EWYDHAKQLTQGG+LVTRVW
Sbjct: 541 SMVLDACRLAGILEKLEQSQKWEIIGNVWVELLGRISCEFEWYDHAKQLTQGGNLVTRVW 600

Query: 601 ILMHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVF-NISSSL 653
           ILMHHLG  K NDVFT H+QEDQQDTTPLLGH+IVADYVVEQMLNV+F NI SSL
Sbjct: 601 ILMHHLGCDKPNDVFTMHMQEDQQDTTPLLGHDIVADYVVEQMLNVIFNNIVSSL 655

BLAST of CSPI01G31600 vs. ExPASy TrEMBL
Match: A0A1S3C8L7 (uncharacterized protein LOC103498043 OS=Cucumis melo OX=3656 GN=LOC103498043 PE=4 SV=1)

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 597/655 (91.15%), Postives = 622/655 (94.96%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           MEIFPPSFW+FMYLWEPSAALFLSLCVQLVLVP+GLKRK SSSQF RFFLLIAY+FSDWI
Sbjct: 1   MEIFPPSFWDFMYLWEPSAALFLSLCVQLVLVPMGLKRKHSSSQFRRFFLLIAYSFSDWI 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           ANFSFVMLVERYGTGCYDDFTDP Y+IRAFLAHFLLLHLGG DTITAYSMEDNELWLRTL
Sbjct: 61  ANFSFVMLVERYGTGCYDDFTDPNYIIRAFLAHFLLLHLGGFDTITAYSMEDNELWLRTL 120

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
           LS+LA+LAASIYIFLQALLPTSLNY+SIPVIIAG+IKN EKIWALRSASAERLRDFLAVS
Sbjct: 121 LSLLAVLAASIYIFLQALLPTSLNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVS 180

Query: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240
           TPSPITTHNEEEVQDFE+L IAYYFF RDKRLFVGLGPTSYDLQQNRL YYEKF+S SAF
Sbjct: 181 TPSPITTHNEEEVQDFEMLHIAYYFFNRDKRLFVGLGPTSYDLQQNRLCYYEKFQSNSAF 240

Query: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300
           KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSL IA LTYCLIDKQEYPSTYVNLI
Sbjct: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAILTYCLIDKQEYPSTYVNLI 300

Query: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360
           FLLF GALSIEIYSLFL LFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWS KKRRCSN
Sbjct: 301 FLLFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSFKKRRCSN 360

Query: 361 SISQYNLISHCLEQKN-DSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQE 420
           SISQYNLISHCL+QKN DSYY KF +TKT+AAFSVQRPISNNLEAHIFQQLK+KLVLNQE
Sbjct: 361 SISQYNLISHCLKQKNDDSYYCKFHNTKTMAAFSVQRPISNNLEAHIFQQLKKKLVLNQE 420

Query: 421 YDYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEE-SKSCIPPQDSVSLSNFL 480
           YD GYNEIGWSLKLDLDQSIL+WHIATDFCY+SSPKFKESEE S+SCIPPQDS+SLSNFL
Sbjct: 421 YDSGYNEIGWSLKLDLDQSILLWHIATDFCYYSSPKFKESEEYSESCIPPQDSISLSNFL 480

Query: 481 AYFIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELKIEVVKEERKE 540
           AYFIVHHPSLFPS MSQIRHKATSE VLELLQD+KL RC SNMLKNLELKIEVVKEERKE
Sbjct: 481 AYFIVHHPSLFPSRMSQIRHKATSEDVLELLQDKKLGRCNSNMLKNLELKIEVVKEERKE 540

Query: 541 SRVLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVW 600
           S VLDA RLAG LEKLEQSQKWEIIGNVWVELLGR+SCE EWYDHAK LTQGG+LVTRVW
Sbjct: 541 SMVLDACRLAGILEKLEQSQKWEIIGNVWVELLGRISCEFEWYDHAKHLTQGGNLVTRVW 600

Query: 601 ILMHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVF-NISSSL 653
           ILMHHLG +K NDVFT H+QEDQQDTTPLLGH+IVADYVVEQMLNV+F NI SSL
Sbjct: 601 ILMHHLGCVKPNDVFTMHMQEDQQDTTPLLGHDIVADYVVEQMLNVIFNNIVSSL 655

BLAST of CSPI01G31600 vs. ExPASy TrEMBL
Match: A0A6J1CKT2 (uncharacterized protein LOC111012216 OS=Momordica charantia OX=3673 GN=LOC111012216 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-133
Identity = 305/685 (44.53%), Postives = 408/685 (59.56%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFL---SLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFS 60
           M++FPP+   F+  W       L   S  +Q++L+P+GL+RK SS+  LR  LL+ Y  +
Sbjct: 1   MQVFPPALLYFLQSWSFGLTFLLPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSA 60

Query: 61  DWIANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWL 120
           DW A  S   LV+ YG+     + D  +     LA F+LLHLGGSDTITAYSMEDN+LW 
Sbjct: 61  DWAATTSLGTLVKFYGS-----YEDAFFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWY 120

Query: 121 RTLLSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFL 180
           R+       +  + YI L AL P  L+++ IP+ +AG+IK  E+IW  RS S +RL D L
Sbjct: 121 RSFFGFFVQVGIAFYILLLALQPQHLDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLL 180

Query: 181 AVSTP-SPIT--------THNEEEVQ--------------DFEVLRIAYYFFIRDKRLFV 240
             +T  SPI          H E  +Q                 +L IAYYFF  +K LFV
Sbjct: 181 LSTTRFSPIQINAAKSKHLHLEFPIQINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFV 240

Query: 241 GLGPTSYDLQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTF 300
            L  TSYDLQQ+ L Y+ +F+S+ AFK+IELELGFMYDFFYTKASI HS  G + RLTT 
Sbjct: 241 DLTLTSYDLQQS-LHYFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTL 300

Query: 301 SSLVIAFLTYCLIDKQ---EYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDWNVIWLLTT 360
            S+V+  +T+ +         P T +  + LL+ GALS+EI S  LFL SDWNVI L  T
Sbjct: 301 FSIVVVIVTFHIDHFDPGFNNPLTNILTLILLY-GALSLEISSFILFLCSDWNVIRL--T 360

Query: 361 QSPSNPLPRLALKLISLCGWSLKKRRCSNSISQYNLISHCLEQKNDSYYFKFPSTKTIA- 420
           +S S  L  L  K IS CGWS+KK R SNS+ QYNLIS CL++     Y K+  T  I+ 
Sbjct: 361 KS-SYSLAHLTFKAISRCGWSVKKYRWSNSVRQYNLISCCLKETKYGRYCKYFRTSYISK 420

Query: 421 AFSVQRPISNNLEAHIFQQLKQKLVLNQE----------YDYGYNEIGWSLKLDLDQSIL 480
             +  R IS+ L+  IFQQL QKL +N+E              YN++GWSL+LD DQSIL
Sbjct: 421 IMTASRNISDELKTRIFQQLTQKLEVNEENRKLPGWILRKHNCYNQLGWSLELDSDQSIL 480

Query: 481 IWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAYFIVHHPSLFPSGMSQIRHKA 540
           +WHIAT+ CYH   + KE+E S +C   +D   LS+FL Y +V+H SLF  GMS+IR   
Sbjct: 481 LWHIATNICYH---RDKETEAS-NCSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCE 540

Query: 541 TSEHVLELLQDEKLDRCRSNMLKN-LELKIEVVKEERKESRVLDAFRLAGFLEKLEQSQK 600
           T +  +E +Q  K     S+  K+ L+L+   V ++   S      RLA  L+ LE  ++
Sbjct: 541 TVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKDAGNSVFFGGCRLARELQGLEGCER 600

Query: 601 WEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVWILMHHLGYLKQNDVFTTHIQE 645
           WEII +VWVE+L  +SCEC WY+HAK+L  GG+L+T VW+LMHHLGY+K  ++ T    +
Sbjct: 601 WEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLTHVWLLMHHLGYIKPANLST---MQ 660

BLAST of CSPI01G31600 vs. ExPASy TrEMBL
Match: A0A7J0H3V1 (Transmembrane protein, putative OS=Actinidia rufa OX=165716 GN=Acr_26g0009820 PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.1e-103
Identity = 244/694 (35.16%), Postives = 371/694 (53.46%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           M+IFP    +    WE  A + LSL +Q++L+ LG +RK ++  ++R  L +AY  +DW+
Sbjct: 1   MQIFPSKLAKLWNKWELRATVVLSLFLQIILIGLGSRRKSNTKNWIRIILWLAYLLADWV 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDP-MYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRT 120
           A  S  +L    G     D   P  Y+I AF A FLLLHLGG DTITAYS+EDNELWLR 
Sbjct: 61  ATVSLGVLANNQGDSNSKDHNSPDSYVIMAFWAPFLLLHLGGPDTITAYSLEDNELWLRH 120

Query: 121 LLSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLR-DFLA 180
           LL ++  +  ++Y+ ++AL P  LN+I+IP+ IAG++K  E+ W LRSAS++  R   L 
Sbjct: 121 LLGLVVQVGVALYVSVRALRPADLNFIAIPMFIAGIVKYGERTWILRSASSQHFRKSLLP 180

Query: 181 VSTPSPITTHNEEE---------------------------------VQDFEVLRIAYYF 240
              P P  +   EE                                 + D ++L  A+ F
Sbjct: 181 RPDPGPNYSKFMEEYMLKETEGFELSWSVTQAPKVVAPDYAAGTNRLIPDAKILNAAHDF 240

Query: 241 FIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLC 300
           F+  KRLF  L  +  DL+++  S++++   K AF++I +ELGF+YD  YTKA++ +S+ 
Sbjct: 241 FLTFKRLFADLILSFQDLEKS-ASFFQENSWKDAFEVIGVELGFVYDVLYTKAAVIYSIW 300

Query: 301 GRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDWNV 360
           G + R T+ SS VIAF+ +C ID   Y    V++ FLL  GA+ +EIY++ L L SDW +
Sbjct: 301 GGILRCTSLSSTVIAFMAFCAIDWHGYSPVDVSITFLLLVGAIGLEIYAILLLLSSDWTL 360

Query: 361 IWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSNSISQYNLISHCLEQKNDSY---YFK 420
            WL+      N L  L   +IS     + K++ SN ++QYNL++ C + K  S      K
Sbjct: 361 FWLI---KHDNKLVYLVYTVISSFKCIISKKKWSNCMAQYNLLNSCFKDKKTSTCCDILK 420

Query: 421 FPSTKTIA---AFSVQRPISNNLEAHIFQQLKQKLVLNQEYDY----------------- 480
           +     I     +   + + + L+  IF QL +K     ++                   
Sbjct: 421 WMGMNEILEKWPWETSKNVPSKLKESIFAQLVEKSKSASDFRVCKQLCSARGDRVLQNWN 480

Query: 481 GYNEIGWSLKLDLDQSILIWHIATDFCYHSSP---------KFKESEESKSCIPPQDSVS 540
             N++GWS++++ D SIL+WHIATD CYHS             K+ +      P + S  
Sbjct: 481 CLNDLGWSVEVEFDHSILLWHIATDLCYHSDKDNHPDKDHHSVKDRDLDSVFDPCEMSKL 540

Query: 541 LSNFLAYFIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKL---DRCRSNMLKNLELKI- 600
           LS+++ Y +V  P++ P+G+ QIR + T   VLE  QD+     +R     L  +++KI 
Sbjct: 541 LSDYMLYILVKRPNMLPNGIGQIRFQDTCAEVLEFSQDKNCMSDERLACKKLLRVDVKIP 600

Query: 601 -EVVKEERKESRVLDAFRLAGFLEKL------EQSQKWEIIGNVWVELLGRLSCECEWYD 617
              VK +R +S + DA +LA  L+ L      E  QKWE+I +VW+E+L   + +C W  
Sbjct: 601 PAEVKGDRSKSLLFDACKLAKALQSLQIEREWEMQQKWEMICHVWLEILSYAASQCMWNQ 660

BLAST of CSPI01G31600 vs. NCBI nr
Match: XP_004139148.1 (uncharacterized protein LOC101222078 [Cucumis sativus] >KGN66604.1 hypothetical protein Csa_007023 [Cucumis sativus])

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 649/652 (99.54%), Postives = 650/652 (99.69%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI
Sbjct: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL
Sbjct: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
           LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS
Sbjct: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180

Query: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240
           TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF
Sbjct: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240

Query: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300
           KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI
Sbjct: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300

Query: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360
           FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN
Sbjct: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360

Query: 361 SISQYNLISHCLEQKNDSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQEY 420
           SISQYNLISHCLEQKNDSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQEY
Sbjct: 361 SISQYNLISHCLEQKNDSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQEY 420

Query: 421 DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAY 480
           DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAY
Sbjct: 421 DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAY 480

Query: 481 FIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELKIEVVKEERKESR 540
           FIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLEL IEVVKEERKESR
Sbjct: 481 FIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELNIEVVKEERKESR 540

Query: 541 VLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVWIL 600
           VLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGR+SCECEWYDHAKQLTQGGSLVTRVWIL
Sbjct: 541 VLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRISCECEWYDHAKQLTQGGSLVTRVWIL 600

Query: 601 MHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVFNISSSL 653
           MHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVFN SSSL
Sbjct: 601 MHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVFNNSSSL 652

BLAST of CSPI01G31600 vs. NCBI nr
Match: KAA0037446.1 (uncharacterized protein E6C27_scaffold277G00320 [Cucumis melo var. makuwa] >TYK01920.1 uncharacterized protein E5676_scaffold808G00060 [Cucumis melo var. makuwa])

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 602/655 (91.91%), Postives = 626/655 (95.57%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           MEIFPPSFW+FMYLWEPSAALFLSLCVQLVLVP+GLKRK SSSQFLRFFLLIAY+FSDWI
Sbjct: 1   MEIFPPSFWDFMYLWEPSAALFLSLCVQLVLVPMGLKRKHSSSQFLRFFLLIAYSFSDWI 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           ANFSFVMLVERYGTGCYDDFTDP Y+IRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL
Sbjct: 61  ANFSFVMLVERYGTGCYDDFTDPNYIIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
           LS+LA+LAASIYIFLQALLPTSLNY+SIPVIIAG+IKN EKIWALRSASAERLRDFLAVS
Sbjct: 121 LSLLAVLAASIYIFLQALLPTSLNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVS 180

Query: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240
           TPSPITTHNEEEVQDFE+L IAYYFF RDKRLFVGLGPTSYDLQQNRLSYYEKF+S SAF
Sbjct: 181 TPSPITTHNEEEVQDFEMLHIAYYFFNRDKRLFVGLGPTSYDLQQNRLSYYEKFQSNSAF 240

Query: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300
           KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSL IA LTYCLIDKQEYPSTYVNLI
Sbjct: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAILTYCLIDKQEYPSTYVNLI 300

Query: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360
           FLLF GALSIEIYSLFL LFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWS KKRRCSN
Sbjct: 301 FLLFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSFKKRRCSN 360

Query: 361 SISQYNLISHCLEQKN-DSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQE 420
           SISQYNLISHCL+QKN DSYY KF +TKT+AAFSVQRPISNNLEAHIFQQLK+KLVLNQE
Sbjct: 361 SISQYNLISHCLKQKNDDSYYCKFHNTKTMAAFSVQRPISNNLEAHIFQQLKKKLVLNQE 420

Query: 421 YDYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEE-SKSCIPPQDSVSLSNFL 480
           YD GYNEIGWSLKLDLDQSIL+WHIATDFCY+SSPKFKESEE S+SCIPPQDS+SLSNFL
Sbjct: 421 YDSGYNEIGWSLKLDLDQSILLWHIATDFCYYSSPKFKESEEYSESCIPPQDSISLSNFL 480

Query: 481 AYFIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELKIEVVKEERKE 540
           AYFIVHHPSLFPSGMSQIRHKATSE VLELLQD+KL RC SNMLKNLELKIEVVKEERKE
Sbjct: 481 AYFIVHHPSLFPSGMSQIRHKATSEDVLELLQDKKLGRCNSNMLKNLELKIEVVKEERKE 540

Query: 541 SRVLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVW 600
           S VLDA RLAG LEKLEQSQKWEIIGNVWVELLGR+SCE EWYDHAKQLTQGG+LVTRVW
Sbjct: 541 SMVLDACRLAGILEKLEQSQKWEIIGNVWVELLGRISCEFEWYDHAKQLTQGGNLVTRVW 600

Query: 601 ILMHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVF-NISSSL 653
           ILMHHLG  K NDVFT H+QEDQQDTTPLLGH+IVADYVVEQMLNV+F NI SSL
Sbjct: 601 ILMHHLGCDKPNDVFTMHMQEDQQDTTPLLGHDIVADYVVEQMLNVIFNNIVSSL 655

BLAST of CSPI01G31600 vs. NCBI nr
Match: XP_008458716.1 (PREDICTED: uncharacterized protein LOC103498043 [Cucumis melo])

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 597/655 (91.15%), Postives = 622/655 (94.96%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           MEIFPPSFW+FMYLWEPSAALFLSLCVQLVLVP+GLKRK SSSQF RFFLLIAY+FSDWI
Sbjct: 1   MEIFPPSFWDFMYLWEPSAALFLSLCVQLVLVPMGLKRKHSSSQFRRFFLLIAYSFSDWI 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           ANFSFVMLVERYGTGCYDDFTDP Y+IRAFLAHFLLLHLGG DTITAYSMEDNELWLRTL
Sbjct: 61  ANFSFVMLVERYGTGCYDDFTDPNYIIRAFLAHFLLLHLGGFDTITAYSMEDNELWLRTL 120

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
           LS+LA+LAASIYIFLQALLPTSLNY+SIPVIIAG+IKN EKIWALRSASAERLRDFLAVS
Sbjct: 121 LSLLAVLAASIYIFLQALLPTSLNYVSIPVIIAGIIKNCEKIWALRSASAERLRDFLAVS 180

Query: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240
           TPSPITTHNEEEVQDFE+L IAYYFF RDKRLFVGLGPTSYDLQQNRL YYEKF+S SAF
Sbjct: 181 TPSPITTHNEEEVQDFEMLHIAYYFFNRDKRLFVGLGPTSYDLQQNRLCYYEKFQSNSAF 240

Query: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300
           KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSL IA LTYCLIDKQEYPSTYVNLI
Sbjct: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLFIAILTYCLIDKQEYPSTYVNLI 300

Query: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360
           FLLF GALSIEIYSLFL LFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWS KKRRCSN
Sbjct: 301 FLLFFGALSIEIYSLFLILFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSFKKRRCSN 360

Query: 361 SISQYNLISHCLEQKN-DSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQE 420
           SISQYNLISHCL+QKN DSYY KF +TKT+AAFSVQRPISNNLEAHIFQQLK+KLVLNQE
Sbjct: 361 SISQYNLISHCLKQKNDDSYYCKFHNTKTMAAFSVQRPISNNLEAHIFQQLKKKLVLNQE 420

Query: 421 YDYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEE-SKSCIPPQDSVSLSNFL 480
           YD GYNEIGWSLKLDLDQSIL+WHIATDFCY+SSPKFKESEE S+SCIPPQDS+SLSNFL
Sbjct: 421 YDSGYNEIGWSLKLDLDQSILLWHIATDFCYYSSPKFKESEEYSESCIPPQDSISLSNFL 480

Query: 481 AYFIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELKIEVVKEERKE 540
           AYFIVHHPSLFPS MSQIRHKATSE VLELLQD+KL RC SNMLKNLELKIEVVKEERKE
Sbjct: 481 AYFIVHHPSLFPSRMSQIRHKATSEDVLELLQDKKLGRCNSNMLKNLELKIEVVKEERKE 540

Query: 541 SRVLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVW 600
           S VLDA RLAG LEKLEQSQKWEIIGNVWVELLGR+SCE EWYDHAK LTQGG+LVTRVW
Sbjct: 541 SMVLDACRLAGILEKLEQSQKWEIIGNVWVELLGRISCEFEWYDHAKHLTQGGNLVTRVW 600

Query: 601 ILMHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVF-NISSSL 653
           ILMHHLG +K NDVFT H+QEDQQDTTPLLGH+IVADYVVEQMLNV+F NI SSL
Sbjct: 601 ILMHHLGCVKPNDVFTMHMQEDQQDTTPLLGHDIVADYVVEQMLNVIFNNIVSSL 655

BLAST of CSPI01G31600 vs. NCBI nr
Match: XP_038880416.1 (uncharacterized protein LOC120072067 [Benincasa hispida])

HSP 1 Score: 525.0 bits (1351), Expect = 9.2e-145
Identity = 313/654 (47.86%), Postives = 375/654 (57.34%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           M+IFP SFW  +Y WEPS +L LSLC+QL L+P+GL RK +S+QFLR FLL AY+F+DWI
Sbjct: 1   MDIFPSSFWVMLYHWEPSISLLLSLCMQLALLPMGLMRKRTSNQFLRLFLLFAYSFADWI 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
            +FSF +LVE+YG+GCYD+FTDP Y+IRA LA FLLLHLGGSDTITAYSMED ELWLRTL
Sbjct: 61  TSFSFGILVEKYGSGCYDEFTDPTYIIRALLAPFLLLHLGGSDTITAYSMEDKELWLRTL 120

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
           L ML  L AS Y+FL AL PTSL Y++IP+ +AG+IK  EKIWALR+ASAERLRDF+AVS
Sbjct: 121 LPMLDQLLASFYLFLLALQPTSLKYVAIPIFVAGIIKYGEKIWALRTASAERLRDFVAVS 180

Query: 181 TPSPITTHNEEEVQDFEVLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAF 240
           TPS I TH++EE++D ++L  AY+FF +DKR+FVGLGPTS+D  QN LSYYE+F SK  F
Sbjct: 181 TPSTIITHDQEELKDVQMLHTAYHFFNKDKRMFVGLGPTSFDRHQNGLSYYEEFNSKLPF 240

Query: 241 KIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLI 300
           KIIELELGFMYDFFYTK SINHS  G LF L TFSSLVIA +TYC+IDKQ          
Sbjct: 241 KIIELELGFMYDFFYTKTSINHSRRGLLFLLITFSSLVIAIVTYCMIDKQ---------- 300

Query: 301 FLLFSGALSIEIYSLFLFLFSDWNVIWLLTTQSPSNPLPRLALKLISLCGWSLKKRRCSN 360
                                                                       
Sbjct: 301 ------------------------------------------------------------ 360

Query: 361 SISQYNLISHCLEQKNDSYYFKFPSTKTIAAFSVQRPISNNLEAHIFQQLKQKLVLNQEY 420
                                                                       
Sbjct: 361 ------------------------------------------------------------ 420

Query: 421 DYGYNEIGWSLKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAY 480
                                                          P+D          
Sbjct: 421 -----------------------------------------------PKD---------- 440

Query: 481 FIVHHPSLFPSGMSQIRHKATSEHVLELLQDEKLDRCRSNMLKNLELKIE---VVKEER- 540
                               TSEHVLELLQD+KL R +S+MLKN+ELKIE   V KE+R 
Sbjct: 481 --------------------TSEHVLELLQDKKLGR-KSSMLKNMELKIEVNNVEKEQRN 440

Query: 541 KESRVLDAFRLAGFLEKLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTR 600
            +S +LD  RLA  LE++E+S+KWEIIGNVW+ELLGR+SCECEWYDHAK LTQGG+L+TR
Sbjct: 541 NKSMLLDGCRLARQLEEIEESKKWEIIGNVWMELLGRISCECEWYDHAKHLTQGGNLLTR 440

Query: 601 VWILMHHLGYLKQNDVFTTHIQEDQQDTTPLLGHEIVADYVVEQMLNVVFNISS 651
           VWILMHHLGY+K ++VFT  ++EDQ    PLL HEI+ DYV+EQM +V+FNI S
Sbjct: 601 VWILMHHLGYVKPSNVFT--MEEDQ----PLLDHEILPDYVLEQMFDVIFNIVS 440

BLAST of CSPI01G31600 vs. NCBI nr
Match: XP_022141971.1 (uncharacterized protein LOC111012216 [Momordica charantia])

HSP 1 Score: 486.9 bits (1252), Expect = 2.8e-133
Identity = 305/685 (44.53%), Postives = 408/685 (59.56%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFL---SLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFS 60
           M++FPP+   F+  W       L   S  +Q++L+P+GL+RK SS+  LR  LL+ Y  +
Sbjct: 1   MQVFPPALLYFLQSWSFGLTFLLPIFSFTLQVILIPMGLRRKYSSNNALRLLLLLFYLSA 60

Query: 61  DWIANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWL 120
           DW A  S   LV+ YG+     + D  +     LA F+LLHLGGSDTITAYSMEDN+LW 
Sbjct: 61  DWAATTSLGTLVKFYGS-----YEDAFFGRLFILAPFMLLHLGGSDTITAYSMEDNDLWY 120

Query: 121 RTLLSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFL 180
           R+       +  + YI L AL P  L+++ IP+ +AG+IK  E+IW  RS S +RL D L
Sbjct: 121 RSFFGFFVQVGIAFYILLLALQPQHLDFLGIPIFVAGIIKYGERIWVFRSTSTQRLPDLL 180

Query: 181 AVSTP-SPIT--------THNEEEVQ--------------DFEVLRIAYYFFIRDKRLFV 240
             +T  SPI          H E  +Q                 +L IAYYFF  +K LFV
Sbjct: 181 LSTTRFSPIQINAAKSKHLHLEFPIQINVDHEATQNPHFSHLHLLHIAYYFFKTNKFLFV 240

Query: 241 GLGPTSYDLQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTF 300
            L  TSYDLQQ+ L Y+ +F+S+ AFK+IELELGFMYDFFYTKASI HS  G + RLTT 
Sbjct: 241 DLTLTSYDLQQS-LHYFMQFDSREAFKVIELELGFMYDFFYTKASIIHSRWGPILRLTTL 300

Query: 301 SSLVIAFLTYCLIDKQ---EYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDWNVIWLLTT 360
            S+V+  +T+ +         P T +  + LL+ GALS+EI S  LFL SDWNVI L  T
Sbjct: 301 FSIVVVIVTFHIDHFDPGFNNPLTNILTLILLY-GALSLEISSFILFLCSDWNVIRL--T 360

Query: 361 QSPSNPLPRLALKLISLCGWSLKKRRCSNSISQYNLISHCLEQKNDSYYFKFPSTKTIA- 420
           +S S  L  L  K IS CGWS+KK R SNS+ QYNLIS CL++     Y K+  T  I+ 
Sbjct: 361 KS-SYSLAHLTFKAISRCGWSVKKYRWSNSVRQYNLISCCLKETKYGRYCKYFRTSYISK 420

Query: 421 AFSVQRPISNNLEAHIFQQLKQKLVLNQE----------YDYGYNEIGWSLKLDLDQSIL 480
             +  R IS+ L+  IFQQL QKL +N+E              YN++GWSL+LD DQSIL
Sbjct: 421 IMTASRNISDELKTRIFQQLTQKLEVNEENRKLPGWILRKHNCYNQLGWSLELDSDQSIL 480

Query: 481 IWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAYFIVHHPSLFPSGMSQIRHKA 540
           +WHIAT+ CYH   + KE+E S +C   +D   LS+FL Y +V+H SLF  GMS+IR   
Sbjct: 481 LWHIATNICYH---RDKETEAS-NCSLLEDGTLLSDFLTYLLVYHHSLFLDGMSEIRFCE 540

Query: 541 TSEHVLELLQDEKLDRCRSNMLKN-LELKIEVVKEERKESRVLDAFRLAGFLEKLEQSQK 600
           T +  +E +Q  K     S+  K+ L+L+   V ++   S      RLA  L+ LE  ++
Sbjct: 541 TVDSAIEFMQQRKSIETTSDACKSMLDLETSTVYKDAGNSVFFGGCRLARELQGLEGCER 600

Query: 601 WEIIGNVWVELLGRLSCECEWYDHAKQLTQGGSLVTRVWILMHHLGYLKQNDVFTTHIQE 645
           WEII +VWVE+L  +SCEC WY+HAK+L  GG+L+T VW+LMHHLGY+K  ++ T    +
Sbjct: 601 WEIINHVWVEMLANISCECRWYEHAKKLRHGGNLLTHVWLLMHHLGYIKPANLST---MQ 660

BLAST of CSPI01G31600 vs. TAIR 10
Match: AT5G45540.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 198.0 bits (502), Expect = 2.4e-50
Identity = 193/785 (24.59%), Postives = 318/785 (40.51%), Query Frame = 0

Query: 2   EIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWIA 61
           ++ PP   +    W     + LSL +Q +L+     R+ ++ +     +  AY  +DW A
Sbjct: 3   DMIPPHLRKLWDKWNIRGVIILSLFLQTILIFFAPSRRRTAKKLFLVLIWSAYLLADWAA 62

Query: 62  NFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLL 121
           +++   + +        +       + AF + FLLLHLGG DTITA ++EDNELW R L 
Sbjct: 63  DYAVGQISDSQEEEAESNKPSKNRELLAFWSPFLLLHLGGPDTITALALEDNELWDRHLF 122

Query: 122 SMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVST 181
           S++    A++Y+ L + +P  L   ++ + + GVIK  E+  AL SAS ++ +D + +  
Sbjct: 123 SLVCQAVATVYVILLS-IPNRLLTPTLIMFVGGVIKYVERTAALFSASLDKFKDSM-LDD 182

Query: 182 PSP------------------------------------ITTHNEEEVQDFEVLRIAYYF 241
           P P                                         + E+   +V++ AY +
Sbjct: 183 PDPGANYAKLMEEYEARKKMNMPTDVIVVKDPEKGREGNTPVRPDNELTALQVIQYAYKY 242

Query: 242 FIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLC 301
           F   K L V L  T+ +  ++R  +++K  ++ A +IIE+ELG +YD  +TKA I H+  
Sbjct: 243 FNIFKGLIVDLIFTNQERDESR-KFFDKLTAEEALRIIEVELGLIYDCLFTKAEILHNWT 302

Query: 302 GRLFRLTTFSSLVIAFLTYCLIDKQEYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDWNV 361
           G +FR      LV +   + +  K +Y    V L + L    ++++  +L +F  SDW +
Sbjct: 303 GAVFRFIALGCLVASLCLFKMNKKDQYDGFDVVLTYALLICGIALDSIALLMFCVSDWTI 362

Query: 362 IWLLTTQSPSNPLPRLALKLIS------LCGWSLKK---------------RRCSNSISQ 421
             L   +        L  ++++         W   K               RR S  +  
Sbjct: 363 ARLRKLKEDLEEKDTLTDRVLNWILDFKTLRWKRSKCSQDGHQVLNRNFMFRRWSEYVHA 422

Query: 422 YNLISHCLE--------------------------------------------------- 481
           YNLI  CL                                                    
Sbjct: 423 YNLIGFCLGIRPKRIHYTKGKIHSFFHQTVHILSIDTAIENATRGTRQFHNWIGRFLSNL 482

Query: 482 QKNDS--------YYFKFPSTKTI-----------------AAFSVQRPISNNLEAHIFQ 541
            K D+        ++  FP    +                   F+V   ++  L   IF 
Sbjct: 483 SKRDNSVIRTGLRWFLFFPQLLGLLIYNFLDFFGIKDLVEEIRFTVSDRLTRELWEFIFT 542

Query: 542 QLKQK--LVLNQEYDYGYNEI--GWSL----------------------KLDLDQSILIW 601
           +++QK     +QE   G +     W+L                      + D DQSIL+W
Sbjct: 543 EVQQKHRFAEDQESAKGISSARGNWTLLETSSKKKEDGTDHTKLLQYVTEKDYDQSILLW 602

Query: 602 HIATDFCYHS--SPKFKESEESKSCIPPQD------SVSLSNFLAYFIVHHPSLFP--SG 606
           HIAT+  Y      K  E EE  +    ++      S  LS+++ Y ++  P+L    SG
Sbjct: 603 HIATELLYQKPIDKKVTEKEEHSTNREKEEHSNREFSKILSDYMMYLLIVQPTLMSAVSG 662

BLAST of CSPI01G31600 vs. TAIR 10
Match: AT5G45530.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 189.5 bits (480), Expect = 8.5e-48
Identity = 185/783 (23.63%), Postives = 319/783 (40.74%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           M++ PP+  + +  W     + +SL  Q  L+ L   RK +S + L   L  AY  +DW 
Sbjct: 1   MQVIPPAIKKILDKWNIRGLVIMSLLFQTSLIFLAPMRKRTSKKLLAAVLWTAYLLADWT 60

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           AN++   + +  G             + A  A FLLLHLGG DTITA ++EDN LW R L
Sbjct: 61  ANYAVSQITKNQGKETEPGDPPKNKKLLALWAPFLLLHLGGPDTITALALEDNALWQRHL 120

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
             +++   A +Y  +Q+L       I++ + I G IK  E+  AL SAS ++ +D +   
Sbjct: 121 FGLVSQALAGVYAVVQSLENVLWPPITL-LFITGTIKYVERTRALYSASLDKFKDRMLQR 180

Query: 181 TPS-----------------------------------PITTHNEEEVQDFEVLRIAYYF 240
             +                                   P     + ++ D E+++  + F
Sbjct: 181 ADAGSNYAKLMEEFASRKMSNLPTEIFLTDEPDKHERPPTLVKPDRDLTDLEIVQYGFKF 240

Query: 241 FIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLC 300
           F   K L V L  +  +  ++R  ++++ +   A +IIE ELGF+Y+  YTK +I H+  
Sbjct: 241 FNTFKGLVVDLIFSFRERDESR-DFFKELKPGEALRIIETELGFLYESMYTKTAILHTGI 300

Query: 301 GRLFRLTTFSSLVIAFLTYCL--IDKQEYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDW 360
           G LFRL +F SL+ +F  +    +  +++    V + ++LF   +++++ S+ +FL SDW
Sbjct: 301 GTLFRLISFGSLLSSFFVFHRRPLKSEDFHGADVVITYVLFIVGIALDLASMVIFLLSDW 360

Query: 361 NVIWLLTTQSPSNPLPRLALKLISLCGWSLK-------------------------KRRC 420
               L   +         +  + SL  W L+                          RR 
Sbjct: 361 TFAVLRNLKDDPE---EKSTSIDSLFNWFLEFRKPRWKKHTCNGNQTHEVLSTGFFTRRW 420

Query: 421 SNSISQYNLISHCLEQKNDSYYFK------------------------------------ 480
           S +I  +N I  CL+ K    + K                                    
Sbjct: 421 SGTIYGFNFIGFCLKAKVSRIHQKRNCNLLVWDYVVSLFDLVIRRIQMMIGWIKNVNRSI 480

Query: 481 ------------------FPSTKTIAA----------------FSV-----------QRP 540
                             +P      A                FSV           + P
Sbjct: 481 RSVLRQWSKKNPMIRCTVYPLYLVFFAGIPEVFRVLWKYIDRIFSVTSYLDGIRFISREP 540

Query: 541 ISNNLEAHIFQQLKQKLVLNQEYDYGYNEIGWSL---------------------KLDLD 600
           ++ N    IF ++K K    +  +    ++ W+                      K+D D
Sbjct: 541 LTKNQWEFIFNEVKDKSGFAETPEVA-KKVSWARGEWALRDSKLMEVDTLMRYIEKVDYD 600

Query: 601 QSILIWHIATDFCYHSSPKFKESEESKSCIPPQD-SVSLSNFLAYFIVHHPSLFP--SGM 606
           QS+L+WHIAT+ C+      K  + S+     ++ S  +S+++ Y ++  P L    +G+
Sbjct: 601 QSLLLWHIATELCFQKEEGGKMEKLSREGYDDREFSKIISDYMMYLLIMRPKLMSEVAGI 660

BLAST of CSPI01G31600 vs. TAIR 10
Match: AT5G45470.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 184.1 bits (466), Expect = 3.6e-46
Identity = 207/852 (24.30%), Postives = 335/852 (39.32%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           +E+ P    +    W    A+ LSL +Q +L+     RK +  + L   +  +Y  +DW 
Sbjct: 2   VEVIPKHIKDVWDRWNIRGAVILSLTLQAILICFSPLRKRTPRRLLIVLVWSSYLLADWS 61

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           ANF+  ++ +  G     D       + A  A FLLLHLGG DTITA+++EDN LWLR +
Sbjct: 62  ANFAVGLISKNQGKDLKPDDPPQDKKVMALWAPFLLLHLGGPDTITAFALEDNALWLRHV 121

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLAVS 180
             ++    A +Y+ + + LP SL  + + V ++G IK  E+  AL SAS ++ RD + + 
Sbjct: 122 FGLVFQAIAGVYVVVMS-LPNSLWVVIVLVFVSGTIKYLERTTALYSASLDKFRDSM-IQ 181

Query: 181 TPSP-------------------------------------------ITTHNEEEVQDFE 240
            P P                                            +   ++++ D E
Sbjct: 182 APDPGPNYAKLMEEYKAKKEARLPTKIVLIDEPDKENRPKKLEHPALASKKRKKDLTDLE 241

Query: 241 VLRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKF-ESKSAFKIIELELGFMYDFFYT 300
           +++ AY FF   K L V L   S+  +   L  +E   + + A +IIE+ELGF+YD  +T
Sbjct: 242 IVQYAYKFFNTFKGLVVNL-IFSFRERDESLEIFENLNDPEEALRIIEIELGFLYDALFT 301

Query: 301 KASINHSLCGRLFRLTTFSSLVIAFLTYCLIDKQ--EYPSTYVNLIFLLFSGALSIEIYS 360
           K +I H+  G + R+    +LV AF+ +     +  ++    V + + LF+  L ++  S
Sbjct: 302 KIAILHTGIGTVSRVFASGTLVAAFIIFHKKPNKGTDFHGADVVVTYTLFAVGLVLDFIS 361

Query: 361 LFLFLFSDWNVI---------------------WLL-----------TTQSPSNPLPRLA 420
           + LFLFSDW                        WLL             ++  +   +  
Sbjct: 362 ILLFLFSDWTCAAYSSLKDDPDELQSWQERCFNWLLKFRKLRWTPQECHKTGMHKCTKEG 421

Query: 421 LKLISLCGWSLKK--------------------------------------RRCSNSISQ 480
           LK   + G   K+                                      RR S SI+ 
Sbjct: 422 LKPCIMKGADKKEGGNKNEGGNKKEGDDKKEAADKCSLVDKHDVLTTRFFLRRWSGSINV 481

Query: 481 YNLISHC----LEQKNDS------------------------------------------ 540
           +N I++     +E+ +D+                                          
Sbjct: 482 FNFIAYATKADVERIHDARGSFRRYSWKIITFPFKKLNFVIKKIFGSIVKLINEVHRRIS 541

Query: 541 -------------------YYFKFPS-----TKTI-------------------AAFSVQ 600
                               YF+F S      K++                     F   
Sbjct: 542 HEVNALSRKHLWARRFLYPIYFEFISRIPHFIKSVWDILSEFFDISDTLDMVHKTLFVHG 601

Query: 601 RPISNNLEAHIFQQLKQK---------------------LVLNQEYDYGYNE-IGWSLKL 610
            P++  L   IF++LK K                     L  N   D    + + +  K+
Sbjct: 602 EPMTRELWKFIFEELKNKSKYGDSPENAKRISLARGEWTLRENLPVDAEREKLVRYVTKV 661

BLAST of CSPI01G31600 vs. TAIR 10
Match: AT5G45460.1 (unknown protein; BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF594) (TAIR:AT5G45470.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 146.0 bits (367), Expect = 1.1e-34
Identity = 112/383 (29.24%), Postives = 180/383 (47.00%), Query Frame = 0

Query: 1   MEIFPPSFWEFMYLWEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWI 60
           +++ P    +    W     +FLSL +Q  L+     RK +  + L   +  +Y  +DW 
Sbjct: 2   VDVIPKHIKDAWDRWNIRGTIFLSLTLQAFLICFSPLRKRTPRRHLIIVIWSSYLLADWS 61

Query: 61  ANFSFVMLVERYGTGCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTL 120
           ANF+  ++ +  G     D       + A  A FLLLHLGG DTITA+++EDN LWLR +
Sbjct: 62  ANFAVGLISKNQGKDLKPDDPPQDKKLMALWAPFLLLHLGGPDTITAFALEDNALWLRNV 121

Query: 121 LSMLAILAASIYIFLQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFL--- 180
             ++    A +Y+ LQ+ LP SL    + V I+G IK  E+  AL SAS ++ RD +   
Sbjct: 122 FGLVFQAIAGVYVVLQS-LPNSLWVTILLVFISGTIKYLERTTALYSASLDKFRDSMIQG 181

Query: 181 ---------------------------------------AVSTPSPITTHNEEEVQDFEV 240
                                                   +  PS  +    +E+   E+
Sbjct: 182 PDPGPNYAKLMEEYKAKKEAKLPTKIILIDEPDKEHRPKKLEHPSLASETKRKELTHLEI 241

Query: 241 LRIAYYFFIRDKRLFVGLGPTSYDLQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKA 300
            + AY FF   K L V L  +  +  Q+   +    + + A +IIE+ELGF+YD  +TK 
Sbjct: 242 AQYAYKFFNTFKGLVVNLIFSFRERDQSIEIFQNLEDPEEALRIIEIELGFLYDALFTKN 301

Query: 301 SINHSLCGRLFRLTTFSSLVIAFLTYCLIDK--QEYPSTYVNLIFLLFSGALSIEIYSLF 339
           ++ H++ G + R+    SLV AF+ +  I    +++    V + ++LF+  L ++  S+ 
Sbjct: 302 AVLHTVLGTVSRVVASGSLVAAFIIFHKISNKGRDFHGADVVITYILFAVGLVLDFISIL 361


HSP 2 Score: 40.8 bits (94), Expect = 4.9e-03
Identity = 15/24 (62.50%), Postives = 19/24 (79.17%), Query Frame = 0

Query: 432 KLDLDQSILIWHIATDFCYHSSPK 456
           K+D DQS+L+WHIAT+FCY    K
Sbjct: 632 KVDYDQSLLMWHIATEFCYQEHKK 655

BLAST of CSPI01G31600 vs. TAIR 10
Match: AT5G45480.1 (Protein of unknown function (DUF594) )

HSP 1 Score: 135.2 bits (339), Expect = 1.9e-31
Identity = 107/349 (30.66%), Postives = 162/349 (46.42%), Query Frame = 0

Query: 15  WEPSAALFLSLCVQLVLVPLGLKRKCSSSQFLRFFLLIAYTFSDWIANFSFVMLVERYGT 74
           W   + L  SL +Q  L+    +RK SS + L  F+  AY  +DW ANF+   + +  G 
Sbjct: 16  WSIRSTLIFSLSLQTFLIFFAPQRKRSSRKVLLSFIWSAYLLADWSANFAAGQISDSQGD 75

Query: 75  GCYDDFTDPMYMIRAFLAHFLLLHLGGSDTITAYSMEDNELWLRTLLSMLAILAASIYIF 134
                       + AF   FLLLHLGG DTITA ++EDNELWLR LL +     A++Y+ 
Sbjct: 76  DPEPGEPKKSAELFAFWVPFLLLHLGGPDTITALALEDNELWLRHLLGLFFQSVATVYVL 135

Query: 135 LQALLPTSLNYISIPVIIAGVIKNSEKIWALRSASAERLRDFLA---------------- 194
           LQ+ LP +L    + V   GVIK  E+  AL  AS ++ +D +                 
Sbjct: 136 LQS-LPNALWKPILLVFATGVIKYVERTLALYLASLDKFKDSMIQRPDPGPNYAKLMEEY 195

Query: 195 -----VSTPSPITTHNEEE--------------VQDFEVLRIAYYFFIRDKRLFVGLGPT 254
                +  P+ I    E E                   +L+ AY +F   K L V L  T
Sbjct: 196 AAKKDMKMPTQIIKVGEPEKDPRDDAPVKPPDGFTPLNILQYAYKYFNIFKGLVVDLIFT 255

Query: 255 SYDLQQNRLSYYEKFESKSAFKIIELELGFMYDFFYTKASINHSLCGRLFRLTTFSSLVI 314
                +++  +++  +++ A +I+E+EL F+Y   YTKA I H+  G LFR      L  
Sbjct: 256 FQQRAESK-RFFDSLKAEEALRILEVELNFIYAALYTKAEILHNWIGFLFRFIALGCLAA 315

Query: 315 AFLTYCLIDKQEYPSTYVNLIFLLFSGALSIEIYSLFLFLFSDWNVIWL 329
           A   +    K++Y    V L + L  G ++++  +L +F  SDW  + L
Sbjct: 316 ALRIFQYKSKKDYSGFDVGLTYALLLGGIALDCIALIMFCASDWTFVRL 362


HSP 2 Score: 89.7 bits (221), Expect = 9.2e-18
Identity = 58/197 (29.44%), Postives = 103/197 (52.28%), Query Frame = 0

Query: 431 LKLDLDQSILIWHIATDFCYHSSPKFKESEESKSCIPPQDSVSLSNFLAYFIVHHPSLFP 490
           +++D DQS+L+WHIAT+  Y +    K +  ++     + S  LS+++ Y ++  P+L  
Sbjct: 667 MEMDYDQSLLVWHIATELLYQTKKGTKANHSAR-----EFSKILSDYMMYLLMMQPTLMS 726

Query: 491 S--GMSQIRHKATSE---------HVLELLQDEKLDRCRSNMLKNLELKIEV------VK 550
           +  G+ +IR + T E         H++  +  +K    +   +  L + +        VK
Sbjct: 727 AVVGIGKIRFRDTCEEAQRFFDRRHIMG-ISAKKAPDAKEASVAILSVAVPAKAEPIDVK 786

Query: 551 EERKESRVLDAFRLAGFLE-----KLEQSQKWEIIGNVWVELLGRLSCECEWYDHAKQLT 606
            +R +S + D   LA  L+     K + S+ W+I+  VWVELL   + +C   +HA QL+
Sbjct: 787 GDRSKSVLFDGAMLAKELKGLRKNKEDDSEMWKIMSQVWVELLSYAATKCGAIEHAAQLS 846

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LZZ20.0e+0099.54DUF4220 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G638510 PE=... [more]
A0A5D3BS410.0e+0091.91DUF4220 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C8L70.0e+0091.15uncharacterized protein LOC103498043 OS=Cucumis melo OX=3656 GN=LOC103498043 PE=... [more]
A0A6J1CKT21.3e-13344.53uncharacterized protein LOC111012216 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A7J0H3V11.1e-10335.16Transmembrane protein, putative OS=Actinidia rufa OX=165716 GN=Acr_26g0009820 PE... [more]
Match NameE-valueIdentityDescription
XP_004139148.10.0e+0099.54uncharacterized protein LOC101222078 [Cucumis sativus] >KGN66604.1 hypothetical ... [more]
KAA0037446.10.0e+0091.91uncharacterized protein E6C27_scaffold277G00320 [Cucumis melo var. makuwa] >TYK0... [more]
XP_008458716.10.0e+0091.15PREDICTED: uncharacterized protein LOC103498043 [Cucumis melo][more]
XP_038880416.19.2e-14547.86uncharacterized protein LOC120072067 [Benincasa hispida][more]
XP_022141971.12.8e-13344.53uncharacterized protein LOC111012216 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G45540.12.4e-5024.59Protein of unknown function (DUF594) [more]
AT5G45530.18.5e-4823.63Protein of unknown function (DUF594) [more]
AT5G45470.13.6e-4624.30Protein of unknown function (DUF594) [more]
AT5G45460.11.1e-3429.24unknown protein; BEST Arabidopsis thaliana protein match is: Protein of unknown ... [more]
AT5G45480.11.9e-3130.66Protein of unknown function (DUF594) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 522..542
NoneNo IPR availablePANTHERPTHR31325:SF207OS08G0149333 PROTEINcoord: 12..606
NoneNo IPR availablePANTHERPTHR31325OS01G0798800 PROTEIN-RELATEDcoord: 12..606
IPR007658Protein of unknown function DUF594PFAMPF04578DUF594coord: 554..605
e-value: 4.2E-17
score: 61.5
IPR025315Domain of unknown function DUF4220PFAMPF13968DUF4220coord: 52..369
e-value: 3.7E-54
score: 184.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G31600.1CSPI01G31600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane