CSPI01G29090 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G29090
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionvacuolar protein sorting-associated protein 27-like isoform X2
LocationChr1: 23580818 .. 23585197 (-)
RNA-Seq ExpressionCSPI01G29090
SyntenyCSPI01G29090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGTATGAGATAGTGTATCCATATAGATAGAATGGTAGTTTTTTTCTTCTTTAATAAAAGAATCCTTTGGAATTATTGTTAATTTGGATAAGACGTCTATTTCCTTTTTCTTTTTATTTATTGTAATATTTCGCAGTCGGAGTTCCCAAGTTCCGCTGCCACTTTCTTCCTCAGCTCGGAACTCGGAGGAGATCCCCGCACCTCCTCTGCCCACCACGGTGGTTCCTCTCCCTCAAAATTATCTGAAAGCATCAATTAAACCAATCGGACAATCGTGCAATCGGCGGATTTTCACAATAATGTCGGTGGACCCGCCTCCTTTCCAAGAAGCAGCACGCTGCGATGTCTGCAAATGTAGTTTCAATACCTTCCGGCGACGGGTACCGAATTTCTGTTTCCTCTATAATATAATCAACTTCTTTCAGATGATTCAGATTGAGCTACTGCGTATATGATCGATTTTGATTCTGTTTTGAGTCTTTTGACTTTTGAGCTGAGATTAGATTTGTTCTGCGTTTTTTTTTTTTTGTTGGAATCTTTGTTTGCAGCATCATTGCCGATGCTGTGGAAGGACGTTGTGTAATGAGCATTCTTCTTTTCAAATGGTATTCTTCTTTTTCTTTTTCTCTTTCTCGTTGATTGATTGGTTTAATGGGGAATTTATTGTGATCTGATTGGTATATTGTGTGTTCTTGTTTGTGTGAAATTCAGGCCTTGCCGCAGTTTGGTATTCATTCCTCTGTTAGAGTTTGTGCTGACTGTTTTAATAACTCCTCACAGTAAGTCATGTTTAAATTTAATCATACGCTCTGTCACATTTTTCTTTCCTTATGCGAAGTCTTTTGCAGCTTCGAACATGAAATTTCATGTTATTAGTTTCAAGTTCTATATTATTTTTCCCATAAAGATACTTGAAAGAAGACGGTTAGAAACTTACAAATTCTAACTCGAACAAATTCATCAGCGGGATAGTTTCGAAACTTTTGAAATTTTGGAGCTTGTTAATAGATATTCAGGACAAGTTAGAATCTGTGGTCCTTGTTTCTGTTGAAGAAGGGGCTTACACTAGTGCGTGCTGTTCATGAAGTTTGCACCTTTGTTTTTTATTCCATTTCTTATTTTTAGCTTTCATGTGAAGAAACCATTGAAATTTTTAGTGGGTTTCCAATCTCATTGACAATGAGAATTTTCTGCTCCTCTGTGAATTAGTGTGACTGGAACCAACATTCTAAAACCTTCTTTGAACAGTCTTTAAAGCTTAACGTCATTCAAAACCACCAATGTTGAGGTAGTTGGATATTGATGAGTGATCTGAAGGCTGATGCTTGTGTGGGATGAAGCATTAAATTTCTTACATCAGCTTTCAGCACATTCTTCTTGGATTTAATTATAAAAAATCATATACAGATTTTGGAAGCTCTTGCCTTCCCTTGTTTCCTCCTAATACTTGGTCTCTGAGGCATCCTTTTGAGCTCTCAATTTTAGGCAAAGATTGAACCTAGTATTTAGCATCTATGCCATATAGGATTACATCTATAGAGTTCAGCTTTTTTAAAAAATTATCTTTCAGTATTTAAACTTTAATGTAAAAATTATCTTCATCATATAAACAAAAACTAGTGTAAATTTATTGAATCTACTTTATTACAGTTCTAAAGGAGTTGGTCAACCACCATCGTTAGCTGTAGTTGATCAAGTAGGAGATGCAGTTTCCCAATTGAACCTTGATGGGGATCTAACTTCTGAGCCTAAACCAGCTGTGGAGCATCAACCTGCGTTGGGAATTCCAGATTGCAAGTGTGGAATGCCATTATGCATTTGTGAAACTCCAGCCCAGCCTATGGAAACAGAAGCAGTATCTTCGCAGGTTTTAATATTCAGTAATTTCTAGTGCTCCTCATGCATCAGCCCTCCCCTGATGAAACTTGATTGTTCTCATGGCAGAAAAAAACAGCTTCTACCTCTACTCAGTACAATTCAAAACCAAAGAAGACTGACTCCAATCTAAGGAATAGAGCATCTACCTCCAACAGCAAGCTTCGGTCTGTTATCTGGAGTTTGTTTTATTCTGATAGGATTGAGCCTGTTTAGATATTTTATTACCCTCTTTATCACTGTTAGTATTATTGAATTTGAAGGATAAGTCCGATTTTAATCTGCAGTTGTAACTCATATCATTAAACAGGAATCTTGCTGATGAATCGAGAATTAATTATGAAGCAAGTGGCGAGGTATATAGTCTGTATCATCTTAATATTCCTTTGATGTCTGTCAAAGGCTTTGAAGTTCCGCTTCATCTTTAGAATTTCATGTTCTTGCAGGGGCTAAGGGAAGCAGTCAAGAATGGTGACACGATTGCTGTTAAGAAACTTTTGAGCGAGGTACTTACTAAATGCGTTTATAAAAATCAAATTATGTAATGGAATTATTGACTCTTCTCATGTTGGCAGGGTGTAGATGCAAATTATCACGATAAACAAGGACTGTCTTTGCTGCATGTGGTATAAAATCTCTCTTTCTTCACCCCACCCAGGAAAACATGGCATTCATTTCAGTTTAGAGGTATAACATCAGGATTAATATTTATTTGGATGTAGGCGGCGGTCTTTAATCAGACCGATATTGTTTTTGCCCTCATTGAAGGCGGGGCTAGCCTGGACTACAAAAATGCTCAAGGTAACTACTTATCAATGTTACTGGATCTTGATAATAGTATCGAGCATAAACTGAGTGTCTTAACTTTTCTATCCATTTATACTTAAGGTGGGTCTTTGAGAATTTTGAGTGTTTAAAAAAACTGTCTTGATAACAGCTTTTGTTTTTGTATTTTATTGTTTTTCTTCTTCATAATATTATTATGTGCATTGAAATTACTGTTTTTATTTTTCAAAAAAGGAATGTCTCATTCAATTATTCAAATGTTTCTTCTGCAAAGTTTCATTCTAAAAGGCAGAGGCTGTTCCAAGAAACAAATTTCAAACAACCCTTGCTCATCAGTTATCAGTTATCCTTTTCTCTTTCTTCAAGCTAGGCGATTAAAAACCAAAGAATAGGAATAAGCATAGCTAGTTTCCGTGTGACTACAGTGAGTATAATGTTCTGGTGGTGTGTGAAAGCTGTTAAATTATGGTTAGCTTCTCTCTAGAAATTAAAGATCTTAACTCACGATTAACTTTTTATATTACTAAGGTATTATTCATTCACCATCTTGAAAAAAAGAAAAAAAACCTTTGGCCGATGTTTGTGACTGAGTTAAAGCTTGGATGTAAGATTCTTAAAGATAAGATCAGGCAATAAGTTTGCTAGAATTTTAAGTTTAGGGATGTGAAATATCTAAGAAATCTCCTATATTTTCCCTTTGGCTTTTGAAAAACTAAGCTTACAAATAGTCTGTGAGTTTCTTTGTTTTGTTATCCACTTTCTAACCATGTTTTTCTTCTAAAATCATGTATAACCTTCAACAATATCTGTTCTATCGGTTGTTTTTTTTAAATTCATGCAAAGTTTGAAAACTGAGAAAAAGTAGTTTTTGAGAACATATTTTTGTTTTTTGGAATCTAGAAATCAAATCTTACCTTAAAAGAGATGAAAGAAAATTTCAGTGTAAAGAACGGTGAGAAAACAAACACAAATTTCAAAACTTACAAAACCAAACAGTGGACTTTCACTTAGAAGTGAGCATAATTAGAGAGCTATGAAAAGGAGAGGATCTCTCTTATTTAGAGTTTTATGCGATTTGTAGACTTACACAAGTACAAGAGAGGAGGACTAAATTAGGGTACAACATTTTGTGATTACCTAAGAAAAAAAGCTGAGACTAGGGGTGAGGGTTAGTTTTTAAGAAAACCTAGTTCAGCTCGAATCAATTCGAATTATAACAGAAAATCGAACCAATTCAGTTTGGTTTCTTACACCCTGCATAATGATTCATTGTTGCCATTGCCACAGGAGAGACACCATTGGATTGTGCTCCAGCTTCTTTGCAATATAAAATACGAGAGAAGATGGAAGGACAATGAACAAATGAAGAGATGACTTAACCATCTTTTTCCAGTAAATACTTCCATAATCTCTGCACTGGTGGCTTTACTTCTAAGTAATTATATAAACCATAAATCTTTAAATTAATTTATCCTTTTAGATGCTCTCTGTAGTGAGCGGTGTTTGAGAGGCTGGATGGAAGCTTAGTTGAAAAGCTGTATTTACTTTCTAAAACAAAATAGGCAGAGAGATATTTGAATGTCACTTTGTCTCTTTAGCTTTTATATTTGTTTTTGTGCTTCCTTTGACTTTTGAGAACTGAGTTGCAAAGTTTGATCTGTGTAAGTTTGTGATATGTGATCTTATTTTATTTTATCTTTGAGTTGGTTAGTCTAGG

mRNA sequence

ATTTGTATGAGATAGTGTATCCATATAGATAGAATGGTAGTTTTTTTCTTCTTTAATAAAAGAATCCTTTGGAATTATTGTTAATTTGGATAAGACGTCTATTTCCTTTTTCTTTTTATTTATTGTAATATTTCGCAGTCGGAGTTCCCAAGTTCCGCTGCCACTTTCTTCCTCAGCTCGGAACTCGGAGGAGATCCCCGCACCTCCTCTGCCCACCACGGTGGTTCCTCTCCCTCAAAATTATCTGAAAGCATCAATTAAACCAATCGGACAATCGTGCAATCGGCGGATTTTCACAATAATGTCGGTGGACCCGCCTCCTTTCCAAGAAGCAGCACGCTGCGATGTCTGCAAATGTAGTTTCAATACCTTCCGGCGACGGCATCATTGCCGATGCTGTGGAAGGACGTTGTGTAATGAGCATTCTTCTTTTCAAATGGCCTTGCCGCAGTTTGGTATTCATTCCTCTGTTAGAGTTTGTGCTGACTGTTTTAATAACTCCTCACATTCTAAAGGAGTTGGTCAACCACCATCGTTAGCTGTAGTTGATCAAGTAGGAGATGCAGTTTCCCAATTGAACCTTGATGGGGATCTAACTTCTGAGCCTAAACCAGCTGTGGAGCATCAACCTGCGTTGGGAATTCCAGATTGCAAGTGTGGAATGCCATTATGCATTTGTGAAACTCCAGCCCAGCCTATGGAAACAGAAGCAGTATCTTCGCAGAAAAAAACAGCTTCTACCTCTACTCAGTACAATTCAAAACCAAAGAAGACTGACTCCAATCTAAGGAATAGAGCATCTACCTCCAACAGCAAGCTTCGGAATCTTGCTGATGAATCGAGAATTAATTATGAAGCAAGTGGCGAGGGGCTAAGGGAAGCAGTCAAGAATGGTGACACGATTGCTGTTAAGAAACTTTTGAGCGAGGGTGTAGATGCAAATTATCACGATAAACAAGGACTGTCTTTGCTGCATGTGGCGGCGGTCTTTAATCAGACCGATATTGTTTTTGCCCTCATTGAAGGCGGGGCTAGCCTGGACTACAAAAATGCTCAAGGAGAGACACCATTGGATTGTGCTCCAGCTTCTTTGCAATATAAAATACGAGAGAAGATGGAAGGACAATGAACAAATGAAGAGATGACTTAACCATCTTTTTCCAGTAAATACTTCCATAATCTCTGCACTGGTGGCTTTACTTCTAAGTAATTATATAAACCATAAATCTTTAAATTAATTTATCCTTTTAGATGCTCTCTGTAGTGAGCGGTGTTTGAGAGGCTGGATGGAAGCTTAGTTGAAAAGCTGTATTTACTTTCTAAAACAAAATAGGCAGAGAGATATTTGAATGTCACTTTGTCTCTTTAGCTTTTATATTTGTTTTTGTGCTTCCTTTGACTTTTGAGAACTGAGTTGCAAAGTTTGATCTGTGTAAGTTTGTGATATGTGATCTTATTTTATTTTATCTTTGAGTTGGTTAGTCTAGG

Coding sequence (CDS)

ATGTCGGTGGACCCGCCTCCTTTCCAAGAAGCAGCACGCTGCGATGTCTGCAAATGTAGTTTCAATACCTTCCGGCGACGGCATCATTGCCGATGCTGTGGAAGGACGTTGTGTAATGAGCATTCTTCTTTTCAAATGGCCTTGCCGCAGTTTGGTATTCATTCCTCTGTTAGAGTTTGTGCTGACTGTTTTAATAACTCCTCACATTCTAAAGGAGTTGGTCAACCACCATCGTTAGCTGTAGTTGATCAAGTAGGAGATGCAGTTTCCCAATTGAACCTTGATGGGGATCTAACTTCTGAGCCTAAACCAGCTGTGGAGCATCAACCTGCGTTGGGAATTCCAGATTGCAAGTGTGGAATGCCATTATGCATTTGTGAAACTCCAGCCCAGCCTATGGAAACAGAAGCAGTATCTTCGCAGAAAAAAACAGCTTCTACCTCTACTCAGTACAATTCAAAACCAAAGAAGACTGACTCCAATCTAAGGAATAGAGCATCTACCTCCAACAGCAAGCTTCGGAATCTTGCTGATGAATCGAGAATTAATTATGAAGCAAGTGGCGAGGGGCTAAGGGAAGCAGTCAAGAATGGTGACACGATTGCTGTTAAGAAACTTTTGAGCGAGGGTGTAGATGCAAATTATCACGATAAACAAGGACTGTCTTTGCTGCATGTGGCGGCGGTCTTTAATCAGACCGATATTGTTTTTGCCCTCATTGAAGGCGGGGCTAGCCTGGACTACAAAAATGCTCAAGGAGAGACACCATTGGATTGTGCTCCAGCTTCTTTGCAATATAAAATACGAGAGAAGATGGAAGGACAATGA

Protein sequence

MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVCADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCGMPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADESRINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALIEGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ*
Homology
BLAST of CSPI01G29090 vs. ExPASy Swiss-Prot
Match: Q6FQJ1 (Vacuolar protein sorting-associated protein 27 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=VPS27 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.5e-11
Identity = 48/168 (28.57%), Postives = 74/168 (44.05%), Query Frame = 0

Query: 2   SVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVCA 61
           S  P  + ++  C +C   F+   RRHHCR CG   C +HSS  + LP  GI+ SVRVC 
Sbjct: 164 SKTPADWIDSDACMICSKKFSLLNRRHHCRSCGGVFCQDHSSKSIPLPDLGIYDSVRVCD 223

Query: 62  DCFNNSSHSKGVGQPPSL---------AVVDQVGDAVSQLNLD---GDLTSEPK-PAVEH 121
           +C+++  + KG G              A  D+  D    + L     + + EP  P VE 
Sbjct: 224 NCYDDYDYKKGSGSQGKKRKHRKSHRHATEDEDEDLRKAIELSLRASNSSVEPVIPVVES 283

Query: 122 QPALGI-----PDCKCGMPLCICETPAQPMETEAVSSQKKTASTSTQY 152
           +P +       PD K  +   + E   +    E  + Q+ T +   Q+
Sbjct: 284 EPQIPAVEEEDPDLKAAIEASLREAEEEKRRREEHAQQQNTNAYPQQF 331

BLAST of CSPI01G29090 vs. ExPASy Swiss-Prot
Match: Q755J9 (Vacuolar protein sorting-associated protein 27 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=VPS27 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.7e-10
Identity = 27/61 (44.26%), Postives = 38/61 (62.30%), Query Frame = 0

Query: 5   PPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVCADCF 64
           P  + ++  C +C  +F    R+HHCR CG   CNEHSS Q+ LP+ GI   VRVC +C+
Sbjct: 172 PADWVDSDACMICSNAFTFLNRKHHCRSCGGIFCNEHSSHQLPLPEMGITEPVRVCDNCY 231

Query: 65  N 66
           +
Sbjct: 232 D 232

BLAST of CSPI01G29090 vs. ExPASy Swiss-Prot
Match: Q17AN2 (Lateral signaling target protein 2 homolog OS=Aedes aegypti OX=7159 GN=AAEL005241 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.8e-10
Identity = 29/58 (50.00%), Postives = 35/58 (60.34%), Query Frame = 0

Query: 7   PFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVCADCF 65
           P  +A RC  C  SF  FRRRHHCR CG   C   SS    LP++G+  +VRVC DC+
Sbjct: 849 PDGDAPRCMACASSFTPFRRRHHCRNCGGVFCGVCSSASAPLPKYGLTKAVRVCRDCY 906

BLAST of CSPI01G29090 vs. ExPASy Swiss-Prot
Match: B4NFJ7 (Lateral signaling target protein 2 homolog OS=Drosophila willistoni OX=7260 GN=GK22512 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.8e-10
Identity = 30/68 (44.12%), Postives = 38/68 (55.88%), Query Frame = 0

Query: 7   PFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVCADCFNN 66
           P  +A RC  C+  F  FRRRHHCR CG   C   S+    LP++G+  +VRVC DC+  
Sbjct: 904 PDGKAPRCMSCQTPFTAFRRRHHCRNCGGVFCGVCSNASAPLPKYGLTKAVRVCRDCYAR 963

Query: 67  SSHSKGVG 75
              S G G
Sbjct: 964 EIRSSGGG 971

BLAST of CSPI01G29090 vs. ExPASy Swiss-Prot
Match: Q7QAJ2 (Lateral signaling target protein 2 homolog OS=Anopheles gambiae OX=7165 GN=AGAP003678 PE=3 SV=6)

HSP 1 Score: 66.6 bits (161), Expect = 4.9e-10
Identity = 30/67 (44.78%), Postives = 39/67 (58.21%), Query Frame = 0

Query: 1    MSVDPP---PFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSV 60
            M  +PP   P  +A RC  C  +F  FRRRHHCR CG   C   S+    LP++G+  +V
Sbjct: 1089 MPEEPPRWIPDCDAPRCMACASAFTPFRRRHHCRNCGGVFCGVCSNLSKPLPKYGLTKAV 1148

Query: 61   RVCADCF 65
            RVC DC+
Sbjct: 1149 RVCRDCY 1155

BLAST of CSPI01G29090 vs. ExPASy TrEMBL
Match: A0A0A0LWS8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G598850 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.4e-153
Identity = 273/275 (99.27%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC
Sbjct: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
           ADCFNNSS SKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG
Sbjct: 61  ADCFNNSSQSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180
           MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES
Sbjct: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180

Query: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240
           RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI
Sbjct: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240

Query: 241 EGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           EGGASLDYKNAQGETP+DCAPASLQYKIREKMEGQ
Sbjct: 241 EGGASLDYKNAQGETPMDCAPASLQYKIREKMEGQ 275

BLAST of CSPI01G29090 vs. ExPASy TrEMBL
Match: A0A5A7UKZ2 (Vacuolar protein sorting-associated protein 27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001170 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-147
Identity = 263/275 (95.64%), Postives = 267/275 (97.09%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSV+PPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLC+EHSSFQMALPQFGIHSSVRVC
Sbjct: 1   MSVEPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFGIHSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
           ADCFNNSS SK VGQPPSLAVVDQVGD VSQL+LDGDL SEPKPAVEHQPALGIPDCKCG
Sbjct: 61  ADCFNNSSQSKVVGQPPSLAVVDQVGDTVSQLSLDGDLNSEPKPAVEHQPALGIPDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180
           MPLCICETPAQPMETEAV SQKKTASTSTQYN KPKKTDSNLRNR STSNSKLRNLADES
Sbjct: 121 MPLCICETPAQPMETEAVPSQKKTASTSTQYNPKPKKTDSNLRNRGSTSNSKLRNLADES 180

Query: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240
           RINYEASGEGLREA+KNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI
Sbjct: 181 RINYEASGEGLREAIKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240

Query: 241 EGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           E GASLDYKNAQGETPLDCAPASLQYKIREKMEGQ
Sbjct: 241 ENGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 275

BLAST of CSPI01G29090 vs. ExPASy TrEMBL
Match: A0A6J1JJ55 (myotubularin-related protein 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485580 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.5e-139
Identity = 250/275 (90.91%), Postives = 256/275 (93.09%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSV+PPPFQEAARC+VCKCSFNTFRRRHHCRCCGRTLC EHSSFQMALPQFGI SSVRVC
Sbjct: 1   MSVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCREHSSFQMALPQFGIQSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
            DCFN SS S+ VGQPPSLAVVDQV D VS+LNLDGD+ SEPKPAVEHQPA GI DCKCG
Sbjct: 61  GDCFNVSSQSREVGQPPSLAVVDQVADTVSKLNLDGDVNSEPKPAVEHQPAFGISDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180
           MPLCICETP Q METEAV SQKKTASTSTQYNSKPKKTDSNLRNR STSNSKLRN ADES
Sbjct: 121 MPLCICETPVQSMETEAVPSQKKTASTSTQYNSKPKKTDSNLRNRGSTSNSKLRNHADES 180

Query: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240
           RINYEASGEGLREA+KNGDTIAVK LLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI
Sbjct: 181 RINYEASGEGLREAIKNGDTIAVKNLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240

Query: 241 EGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           E GASLDYKNAQGETPLDCAPASLQYKIREKM GQ
Sbjct: 241 ESGASLDYKNAQGETPLDCAPASLQYKIREKMGGQ 275

BLAST of CSPI01G29090 vs. ExPASy TrEMBL
Match: A0A6J1H9N2 (vacuolar protein sorting-associated protein 27-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461389 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-138
Identity = 251/276 (90.94%), Postives = 259/276 (93.84%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSV+PPPFQEAARC+VCKCSFNTFRRRHHCRCCGRTLC+EHSSFQMALPQFGI SSVRVC
Sbjct: 1   MSVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFGIQSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
            DCFN SS S+ VGQPPSLAVVDQV D VS+LNLDGD+ SEPKPAVEHQPA GI DCKCG
Sbjct: 61  GDCFNVSSQSREVGQPPSLAVVDQVADTVSKLNLDGDVNSEPKPAVEHQPAFGISDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNL-ADE 180
           MPLCICETP Q METEAV SQKKTASTSTQY+SKPKKTDSNLRNR STSNSKLRNL ADE
Sbjct: 121 MPLCICETPVQSMETEAVPSQKKTASTSTQYSSKPKKTDSNLRNRGSTSNSKLRNLAADE 180

Query: 181 SRINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL 240
           SRINYEASGEGLREA+KNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL
Sbjct: 181 SRINYEASGEGLREAIKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL 240

Query: 241 IEGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           IE GASLDYKNAQGETPLDCAPASLQYKIREKM GQ
Sbjct: 241 IESGASLDYKNAQGETPLDCAPASLQYKIREKMGGQ 276

BLAST of CSPI01G29090 vs. ExPASy TrEMBL
Match: A0A6J1JGT2 (myotubularin-related protein 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485580 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.2e-137
Identity = 249/275 (90.55%), Postives = 255/275 (92.73%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSV+PPPFQEAARC+VCKCSFNTFRRRHHCRCCGRTLC EHSSFQMALPQFGI SSVRVC
Sbjct: 1   MSVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCREHSSFQMALPQFGIQSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
            DCFN SS S+ VGQPPSLAVVDQV D VS+LNLDGD+ SEPKPAVEHQPA GI DCKCG
Sbjct: 61  GDCFNVSSQSREVGQPPSLAVVDQVADTVSKLNLDGDVNSEPKPAVEHQPAFGISDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180
           MPLCICETP Q METEAV SQ KTASTSTQYNSKPKKTDSNLRNR STSNSKLRN ADES
Sbjct: 121 MPLCICETPVQSMETEAVPSQ-KTASTSTQYNSKPKKTDSNLRNRGSTSNSKLRNHADES 180

Query: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240
           RINYEASGEGLREA+KNGDTIAVK LLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI
Sbjct: 181 RINYEASGEGLREAIKNGDTIAVKNLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240

Query: 241 EGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           E GASLDYKNAQGETPLDCAPASLQYKIREKM GQ
Sbjct: 241 ESGASLDYKNAQGETPLDCAPASLQYKIREKMGGQ 274

BLAST of CSPI01G29090 vs. NCBI nr
Match: XP_004144059.1 (vacuolar protein sorting-associated protein 27 [Cucumis sativus] >KGN66340.1 hypothetical protein Csa_007134 [Cucumis sativus])

HSP 1 Score: 552.0 bits (1421), Expect = 3.0e-153
Identity = 273/275 (99.27%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC
Sbjct: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
           ADCFNNSS SKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG
Sbjct: 61  ADCFNNSSQSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180
           MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES
Sbjct: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180

Query: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240
           RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI
Sbjct: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240

Query: 241 EGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           EGGASLDYKNAQGETP+DCAPASLQYKIREKMEGQ
Sbjct: 241 EGGASLDYKNAQGETPMDCAPASLQYKIREKMEGQ 275

BLAST of CSPI01G29090 vs. NCBI nr
Match: KAA0055738.1 (vacuolar protein sorting-associated protein 27 [Cucumis melo var. makuwa] >TYK09987.1 vacuolar protein sorting-associated protein 27 [Cucumis melo var. makuwa])

HSP 1 Score: 532.3 bits (1370), Expect = 2.4e-147
Identity = 263/275 (95.64%), Postives = 267/275 (97.09%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSV+PPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLC+EHSSFQMALPQFGIHSSVRVC
Sbjct: 1   MSVEPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFGIHSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
           ADCFNNSS SK VGQPPSLAVVDQVGD VSQL+LDGDL SEPKPAVEHQPALGIPDCKCG
Sbjct: 61  ADCFNNSSQSKVVGQPPSLAVVDQVGDTVSQLSLDGDLNSEPKPAVEHQPALGIPDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180
           MPLCICETPAQPMETEAV SQKKTASTSTQYN KPKKTDSNLRNR STSNSKLRNLADES
Sbjct: 121 MPLCICETPAQPMETEAVPSQKKTASTSTQYNPKPKKTDSNLRNRGSTSNSKLRNLADES 180

Query: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240
           RINYEASGEGLREA+KNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI
Sbjct: 181 RINYEASGEGLREAIKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240

Query: 241 EGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           E GASLDYKNAQGETPLDCAPASLQYKIREKMEGQ
Sbjct: 241 ENGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 275

BLAST of CSPI01G29090 vs. NCBI nr
Match: XP_038878461.1 (vacuolar protein sorting-associated protein 27 [Benincasa hispida])

HSP 1 Score: 504.2 bits (1297), Expect = 7.1e-139
Identity = 250/274 (91.24%), Postives = 258/274 (94.16%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSV+PPPFQEAARC+VCKCSFNTFRRRHHCRCCGRTLC+EHSSFQMALPQFGIH SVRVC
Sbjct: 1   MSVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFGIHFSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
           ADCFNNSS S+   QPP LAVVDQV D VSQL+LD D+ SE KPAVEHQPALGIPDCKCG
Sbjct: 61  ADCFNNSSQSRDDVQPPLLAVVDQVADTVSQLSLDQDVNSESKPAVEHQPALGIPDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNLADES 180
           MPLCICETP Q METEAV SQ+KTASTSTQYNSKPKKTDSNLRNR STSNSKLRNLADES
Sbjct: 121 MPLCICETPVQSMETEAVPSQRKTASTSTQYNSKPKKTDSNLRNRGSTSNSKLRNLADES 180

Query: 181 RINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240
           RINYEASGEGLREA+KNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI
Sbjct: 181 RINYEASGEGLREAIKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALI 240

Query: 241 EGGASLDYKNAQGETPLDCAPASLQYKIREKMEG 275
           E GASLDYKNAQGETPLDCAPASLQYKIREKMEG
Sbjct: 241 ESGASLDYKNAQGETPLDCAPASLQYKIREKMEG 274

BLAST of CSPI01G29090 vs. NCBI nr
Match: XP_023516961.1 (vacuolar protein sorting-associated protein 27-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 504.2 bits (1297), Expect = 7.1e-139
Identity = 252/276 (91.30%), Postives = 259/276 (93.84%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSV+PPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLC+EHSSFQMALPQFGI SSVRVC
Sbjct: 1   MSVEPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFGIQSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
            DCFN SS S+ VGQPPSLAVVDQV D VS+LNLDGD+ SEPKPAVEHQPA GI DCKCG
Sbjct: 61  GDCFNVSSQSREVGQPPSLAVVDQVADTVSKLNLDGDVNSEPKPAVEHQPAFGISDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNL-ADE 180
           MPLCICETP Q METEAV SQKKTASTSTQYNSKPKK+DSNLRNR STSNSKLRNL ADE
Sbjct: 121 MPLCICETPVQSMETEAVPSQKKTASTSTQYNSKPKKSDSNLRNRGSTSNSKLRNLAADE 180

Query: 181 SRINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL 240
           SRINYEASGEGLREA+KNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL
Sbjct: 181 SRINYEASGEGLREAIKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL 240

Query: 241 IEGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           IE GASLDYKNAQGETPLDCAPASLQYKIREKM GQ
Sbjct: 241 IESGASLDYKNAQGETPLDCAPASLQYKIREKMGGQ 276

BLAST of CSPI01G29090 vs. NCBI nr
Match: KAG6590065.1 (Lateral signaling target protein 2-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 504.2 bits (1297), Expect = 7.1e-139
Identity = 252/276 (91.30%), Postives = 259/276 (93.84%), Query Frame = 0

Query: 1   MSVDPPPFQEAARCDVCKCSFNTFRRRHHCRCCGRTLCNEHSSFQMALPQFGIHSSVRVC 60
           MSV+PPPFQEAARC+VCKCSFNTFRRRHHCRCCGRTLC+EHSSFQMALPQFGI SSVRVC
Sbjct: 1   MSVEPPPFQEAARCEVCKCSFNTFRRRHHCRCCGRTLCHEHSSFQMALPQFGIQSSVRVC 60

Query: 61  ADCFNNSSHSKGVGQPPSLAVVDQVGDAVSQLNLDGDLTSEPKPAVEHQPALGIPDCKCG 120
            DCFN SS S+ VGQPPSLAVVDQV D VS+LNLDGD+ SEPKPAVEHQPA GI DCKCG
Sbjct: 61  GDCFNVSSQSREVGQPPSLAVVDQVADTVSKLNLDGDVNSEPKPAVEHQPAFGISDCKCG 120

Query: 121 MPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRNL-ADE 180
           MPLCICETP Q METEAV SQKKTASTSTQYNSKPKKTDSNLRNR STSNSKLRNL ADE
Sbjct: 121 MPLCICETPVQSMETEAVPSQKKTASTSTQYNSKPKKTDSNLRNRGSTSNSKLRNLAADE 180

Query: 181 SRINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL 240
           SRINYEASGEGLREA+KNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL
Sbjct: 181 SRINYEASGEGLREAIKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFAL 240

Query: 241 IEGGASLDYKNAQGETPLDCAPASLQYKIREKMEGQ 276
           IE GASLDYKNAQGETPLDCAPASLQYKIREKM GQ
Sbjct: 241 IESGASLDYKNAQGETPLDCAPASLQYKIREKMGGQ 276

BLAST of CSPI01G29090 vs. TAIR 10
Match: AT2G26210.1 (Ankyrin repeat family protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-40
Identity = 88/162 (54.32%), Postives = 116/162 (71.60%), Query Frame = 0

Query: 116 DCKCGMPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRN 175
           +CKCGMPLCIC  P  P  T+    +    +T         K++++ +++ STS+S  R+
Sbjct: 33  ECKCGMPLCICVAP--PKSTD----KPNPPATIAPVVLPQLKSEASAKSKGSTSSSNARS 92

Query: 176 L----ADESRINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFN 235
                 D  + +YEASGEGLREA+KNGD+   KKLL EGVDANY D+QG+S+LH+A +FN
Sbjct: 93  ALNAGLDTPQRDYEASGEGLREAIKNGDSAGAKKLLKEGVDANYRDRQGMSVLHLAVLFN 152

Query: 236 QTDIVFALIEGGASLDYKNAQGETPLDCAPASLQYKIREKME 274
           QTDI   L++ GASL+YKNAQGETPLDCAPA+LQYK+REKM+
Sbjct: 153 QTDIALMLMDHGASLEYKNAQGETPLDCAPATLQYKMREKMK 188

BLAST of CSPI01G29090 vs. TAIR 10
Match: AT2G26210.2 (Ankyrin repeat family protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-40
Identity = 88/162 (54.32%), Postives = 116/162 (71.60%), Query Frame = 0

Query: 116 DCKCGMPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRN 175
           +CKCGMPLCIC  P  P  T+    +    +T         K++++ +++ STS+S  R+
Sbjct: 10  ECKCGMPLCICVAP--PKSTD----KPNPPATIAPVVLPQLKSEASAKSKGSTSSSNARS 69

Query: 176 L----ADESRINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFN 235
                 D  + +YEASGEGLREA+KNGD+   KKLL EGVDANY D+QG+S+LH+A +FN
Sbjct: 70  ALNAGLDTPQRDYEASGEGLREAIKNGDSAGAKKLLKEGVDANYRDRQGMSVLHLAVLFN 129

Query: 236 QTDIVFALIEGGASLDYKNAQGETPLDCAPASLQYKIREKME 274
           QTDI   L++ GASL+YKNAQGETPLDCAPA+LQYK+REKM+
Sbjct: 130 QTDIALMLMDHGASLEYKNAQGETPLDCAPATLQYKMREKMK 165

BLAST of CSPI01G29090 vs. TAIR 10
Match: AT2G26210.3 (Ankyrin repeat family protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-40
Identity = 88/162 (54.32%), Postives = 116/162 (71.60%), Query Frame = 0

Query: 116 DCKCGMPLCICETPAQPMETEAVSSQKKTASTSTQYNSKPKKTDSNLRNRASTSNSKLRN 175
           +CKCGMPLCIC  P  P  T+    +    +T         K++++ +++ STS+S  R+
Sbjct: 10  ECKCGMPLCICVAP--PKSTD----KPNPPATIAPVVLPQLKSEASAKSKGSTSSSNARS 69

Query: 176 L----ADESRINYEASGEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFN 235
                 D  + +YEASGEGLREA+KNGD+   KKLL EGVDANY D+QG+S+LH+A +FN
Sbjct: 70  ALNAGLDTPQRDYEASGEGLREAIKNGDSAGAKKLLKEGVDANYRDRQGMSVLHLAVLFN 129

Query: 236 QTDIVFALIEGGASLDYKNAQGETPLDCAPASLQYKIREKME 274
           QTDI   L++ GASL+YKNAQGETPLDCAPA+LQYK+REKM+
Sbjct: 130 QTDIALMLMDHGASLEYKNAQGETPLDCAPATLQYKMREKMK 165

BLAST of CSPI01G29090 vs. TAIR 10
Match: AT3G58760.1 (Integrin-linked protein kinase family )

HSP 1 Score: 50.8 bits (120), Expect = 2.0e-06
Identity = 24/60 (40.00%), Postives = 34/60 (56.67%), Query Frame = 0

Query: 198 GDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALIEGGASLDYKNAQGETPL 257
           GD   + K+L  G + +Y D    + LHVAA   +TD+V  L+  GA +D K+  G TPL
Sbjct: 60  GDIDGINKMLDSGTNVDYRDIDARTALHVAACQGRTDVVELLLSRGAKVDTKDRWGSTPL 119

BLAST of CSPI01G29090 vs. TAIR 10
Match: AT4G18950.1 (Integrin-linked protein kinase family )

HSP 1 Score: 49.7 bits (117), Expect = 4.4e-06
Identity = 26/69 (37.68%), Postives = 36/69 (52.17%), Query Frame = 0

Query: 188 GEGLREAVKNGDTIAVKKLLSEGVDANYHDKQGLSLLHVAAVFNQTDIVFALIEGGASLD 247
           G  L      GD   +K+L+  G+DANY D    + LHVAA     D+V  L++  A +D
Sbjct: 44  GVRLMYLANEGDIEGIKELIDSGIDANYRDIDDRTALHVAACQGLKDVVELLLDRKAEVD 103

Query: 248 YKNAQGETP 257
            K+  G TP
Sbjct: 104 PKDRWGSTP 112

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6FQJ11.5e-1128.57Vacuolar protein sorting-associated protein 27 OS=Candida glabrata (strain ATCC ... [more]
Q755J91.7e-1044.26Vacuolar protein sorting-associated protein 27 OS=Ashbya gossypii (strain ATCC 1... [more]
Q17AN23.8e-1050.00Lateral signaling target protein 2 homolog OS=Aedes aegypti OX=7159 GN=AAEL00524... [more]
B4NFJ73.8e-1044.12Lateral signaling target protein 2 homolog OS=Drosophila willistoni OX=7260 GN=G... [more]
Q7QAJ24.9e-1044.78Lateral signaling target protein 2 homolog OS=Anopheles gambiae OX=7165 GN=AGAP0... [more]
Match NameE-valueIdentityDescription
A0A0A0LWS81.4e-15399.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G598850 PE=4 SV=1[more]
A0A5A7UKZ21.2e-14795.64Vacuolar protein sorting-associated protein 27 OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1JJ554.5e-13990.91myotubularin-related protein 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1H9N21.3e-13890.94vacuolar protein sorting-associated protein 27-like isoform X1 OS=Cucurbita mosc... [more]
A0A6J1JGT24.2e-13790.55myotubularin-related protein 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_004144059.13.0e-15399.27vacuolar protein sorting-associated protein 27 [Cucumis sativus] >KGN66340.1 hyp... [more]
KAA0055738.12.4e-14795.64vacuolar protein sorting-associated protein 27 [Cucumis melo var. makuwa] >TYK09... [more]
XP_038878461.17.1e-13991.24vacuolar protein sorting-associated protein 27 [Benincasa hispida][more]
XP_023516961.17.1e-13991.30vacuolar protein sorting-associated protein 27-like isoform X1 [Cucurbita pepo s... [more]
KAG6590065.17.1e-13991.30Lateral signaling target protein 2-like protein, partial [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
AT2G26210.11.2e-4054.32Ankyrin repeat family protein [more]
AT2G26210.21.2e-4054.32Ankyrin repeat family protein [more]
AT2G26210.31.2e-4054.32Ankyrin repeat family protein [more]
AT3G58760.12.0e-0640.00Integrin-linked protein kinase family [more]
AT4G18950.14.4e-0637.68Integrin-linked protein kinase family [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000306FYVE zinc fingerSMARTSM00064fyve_4coord: 1..69
e-value: 2.1E-16
score: 70.5
IPR000306FYVE zinc fingerPFAMPF01363FYVEcoord: 9..67
e-value: 6.3E-14
score: 51.9
IPR036770Ankyrin repeat-containing domain superfamilyGENE3D1.25.40.20coord: 134..269
e-value: 2.5E-18
score: 68.1
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILY48403Ankyrin repeatcoord: 191..262
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 4..71
e-value: 1.4E-17
score: 65.3
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 193..263
e-value: 5.4E-8
score: 33.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 137..174
NoneNo IPR availablePANTHERPTHR47794VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 27coord: 2..273
NoneNo IPR availablePROSITEPS50297ANK_REP_REGIONcoord: 219..250
score: 9.993605
NoneNo IPR availableCDDcd15760FYVE_scVPS27p_likecoord: 7..64
e-value: 2.49473E-23
score: 88.1227
IPR017455Zinc finger, FYVE-relatedPROSITEPS50178ZF_FYVEcoord: 8..68
score: 11.714371
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 219..251
score: 11.19366
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 8..69

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G29090.1CSPI01G29090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006623 protein targeting to vacuole
biological_process GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component GO:0033565 ESCRT-0 complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0032266 phosphatidylinositol-3-phosphate binding
molecular_function GO:0043130 ubiquitin binding
molecular_function GO:0005515 protein binding