CSPI01G28240 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G28240
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionTranslation machinery-associated protein 22
LocationChr1: 22968749 .. 22971788 (-)
RNA-Seq ExpressionCSPI01G28240
SyntenyCSPI01G28240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTGTTTAATTTATATGGAAAAAATAAATTAGAAAATAGCGTTCTAACGCAAAATACCCTTTCACTGTTCATTGCCCCTCCCAATCTCATTCTCAAGCAACAAACCAAAGAACAGCGAAGGAAAAAGAAAATCTCTTTCGCACCAACAAGGGGGCGGAGAGACTGTTCTTGAGGTGCTTCAATGGCGGAGAAACCCCAACCGGTGCGCGTTTTGTACTGTCCGGTCTGCAGTCTACCGGCCGAGTATTGTGAATTTGGACCCGATTTCGAGAAATGCAAGCCCTGGTTGATCCAAAATGCTCCCGACCTCTATCCTGATCTCCTCAAAGGTTCTTCCCACTCCCTAACCCTAATCCCAACTCTTACTTCATATCCCAGTTTATTTGATTAGCGTTTCTTTGTCTGCAGAGGCCAATGCGAAGGAAGCTGGTGAAGTTTCTAATCAACTGCAGTCCACCAGTATCTCATCTGCTGCTGGCGATGGAGCTGCTTCTTCAGGTATACTTGTCAATTTTGTTTATGTGTGTGTTTGAATCTATGTGTATAGTTTTTTGTTTTGTTGTAATTATATGTTAGGTTCAGTTGAATGAACTCACCACCTGTTGATTCGATTTAGTTCATTACGAAGGCTATTTAGCATTGTAAATGGGTAGGGGTAGTGGATAATCTATTTTCCTAGTAGTATTAGGTGTTGGCGGTGCTTTCCTTGCACGTTTGAGATCCGGGGCCATTGAATAATGTTTTCCATGATTTGGTTATGTGTGGAAAGATAGAAATAATATTATTATCATTTTTGGTAAATTATGGAATTCCTTAGGAGGTTAGGTACGATTGTTCTGCCAAAGAGTTTGCTGGAAACTGGGATCAATTGCATTGTTTGAGTTTGAGCAGATTTAACTTTGTGATCCAACCTGCCTAGAGTTAGTGTTTCTGACATTGGAACTTTGTGGATAGTCGGATATTGAACATTTAATACCAGTATGTGCACTGCTTAGAAAAGCCATCCTGGGCGCCCGCCAAAGCTCTAGGCGTAGGTTTGCACCCTCGGCTTGAAAAAGCAAGGCAAGGCTCACAAAATCAGATGCGTGCTCTTTGTGAAGCCTCAAGGATCAAAGCCTTGGACCTCAAGGATCAAATAATTGGGGGTTTTCTTTCTTTATTTCAACTGTGGTTTCTCTTCTTAATGTAATCCATTTATATGTAGTGCGGCTCACAAATAAAAACTGCATTCTATTTTTCTTTTTTGTGCTTTGTGCTTTGTGCTTAGGCCCCAGAAGACTATTGAACTTTATTGCACCTTGAGTGAGCGAGTTTATGTATGATTAATTGATCCTAAAATATATTTCTTGAAGTTAGGCAAATCATTTTCATCTCAAAGACACTGCAGCAAATAATCCCATAGATCATTTTTCCCCTTTTGCCAATTGGTTCGCTTTCATACCCCTATTCTCTAGGTAGCTATTGATTTGGTTGTCTTATGTTTGACATGGTCTGATTGTATGGTATTTTAACTGATGTTTGCTTTTAATAATTTTATATTTGTTTGGTCTCAACCTATGACTTATAACTCTAGTCATTCTTTTCTCTTTTGGTGGTAAACTACAGGACCTAAGCAAGAGGAAGTGAAACGTCTTCCCGGTGGCAAGATAAAGAAAAAAGTAATTTAGTCACTAAATTCAATCTTATTGTCACTAATAAATAAAGATTCATACGATGGAACTAATTTATTGGATGTACATATAGGATAAGCAAGAAGTTATTATTGAGAAGGTTACACGAAATAAGAGAAAGTGCATTACAACTGTAAAAGGATTAGACCTTTTTGGTGAGCAATACTTTGTTTCCTACTCCTACTTAACATTTCTTCATTGTTACATTTATTTCCAATAATAATGTCTCTTAACTGGATTTAGGAGTTAAGCTAAGTGATGCTTCAAAGAAACTTGGGAAAAAGTTTGCTACTGGTGCTTCTGTGGTTAAGGTTGTTAATTTCTTGTGTCATGACTTAGATATCTGTCTACTGTAGATGCCTCTTGATTTCTTATCATCTGTTTAATACTTGGTTTTAGGGGCCAACTGAGAAAGAGCAAATTGATGTTCAAGGTGACATAGCCTATGACATCGTGGATTTCATTACGGAGACTTGGCCTGATGTAAGATACTTATTCATTATTTTATTTATTTATTATTATTATTTTTGGATAGCCATGAGTTAGAGCTAGGCTCCCCTGTACAATCTGGGAGAAAAGCATATACCAGCCTCCCATTGCCTCTGAACTCATTGCAGCATTGTACTTGAATTTTTTGCTTATACTGTACCATATATTTTTGAATTTGGTTTGGTTATCATGGATGTAGTTAGTTTTTTAAAAATCGTCAACACTTTCAACTTCTAATGATAAAAATCCGAATGGGTGCTTTTTGTTTTTTTCTGAAAGTACAATGTAAAAGGTCTACATGGTCAAATATGTTTCTCTATTATCTAGTCATTCTATAATTATATCAATTTTGTGGCATATTATTTAACCAAAATAATAACAACAATAATATGATGATAAATGAAAGAAACAACTTTATTGAAGTTTAGCTTTGAATTTTGATGTAACTTTATTTGCCATCCTGAATTCTTAGAATTTATTTGTGTCATGAACCAAGGGAGTATGGGATGCCTTTGTCCCACATTGGTTAGAATGCAATGACTAATGTGGTACTTAAGTAGCTTGGCTCTCCCACCCCAATAGCTAGTTTTTGGGGTGTGGTTCTCCAAGGTGCTTAAGTACCTAGCAATGGTATCAGAATCGATTATCGATGTTTCGAAGTGGAACTGATCAGGTGTGACTGAGTGAAGTGTTGTTGGAGTAGCCGTTGGGACTTCGACTTTTCAGGGATGGGGTATTGTTAGGAACCAAGGTTGTATGGGATGTCTTTGTCCCACATTGGTTAGAATGGGAGGACCAATGTGGTACTTGAGTAGCTTGGCTCTCCCACCCCAACAACTGCTTTTGGAGTGTGGTTCTCCAATGTGCTTAAGTACATAA

mRNA sequence

AAATTGTTTAATTTATATGGAAAAAATAAATTAGAAAATAGCGTTCTAACGCAAAATACCCTTTCACTGTTCATTGCCCCTCCCAATCTCATTCTCAAGCAACAAACCAAAGAACAGCGAAGGAAAAAGAAAATCTCTTTCGCACCAACAAGGGGGCGGAGAGACTGTTCTTGAGGTGCTTCAATGGCGGAGAAACCCCAACCGGTGCGCGTTTTGTACTGTCCGGTCTGCAGTCTACCGGCCGAGTATTGTGAATTTGGACCCGATTTCGAGAAATGCAAGCCCTGGTTGATCCAAAATGCTCCCGACCTCTATCCTGATCTCCTCAAAGAGGCCAATGCGAAGGAAGCTGGTGAAGTTTCTAATCAACTGCAGTCCACCAGTATCTCATCTGCTGCTGGCGATGGAGCTGCTTCTTCAGGACCTAAGCAAGAGGAAGTGAAACGTCTTCCCGGTGGCAAGATAAAGAAAAAAGATAAGCAAGAAGTTATTATTGAGAAGGTTACACGAAATAAGAGAAAGTGCATTACAACTGTAAAAGGATTAGACCTTTTTGGAGTTAAGCTAAGTGATGCTTCAAAGAAACTTGGGAAAAAGTTTGCTACTGGTGCTTCTGTGGTTAAGGGGCCAACTGAGAAAGAGCAAATTGATGTTCAAGGTGACATAGCCTATGACATCGTGGATTTCATTACGGAGACTTGGCCTGATCTAGTTTTTGGGGTGTGGTTCTCCAAGGTGCTTAAGTACCTAGCAATGGGATGGGGTATTGTTAGGAACCAAGGTTGTATGGGATGTCTTTGTCCCACATTGGTTAGAATGGGAGGACCAATGTGGTACTTGAGTAGCTTGGCTCTCCCACCCCAACAACTGCTTTTGGAGTGTGGTTCTCCAATGTGCTTAAGTACATAA

Coding sequence (CDS)

ATGGCGGAGAAACCCCAACCGGTGCGCGTTTTGTACTGTCCGGTCTGCAGTCTACCGGCCGAGTATTGTGAATTTGGACCCGATTTCGAGAAATGCAAGCCCTGGTTGATCCAAAATGCTCCCGACCTCTATCCTGATCTCCTCAAAGAGGCCAATGCGAAGGAAGCTGGTGAAGTTTCTAATCAACTGCAGTCCACCAGTATCTCATCTGCTGCTGGCGATGGAGCTGCTTCTTCAGGACCTAAGCAAGAGGAAGTGAAACGTCTTCCCGGTGGCAAGATAAAGAAAAAAGATAAGCAAGAAGTTATTATTGAGAAGGTTACACGAAATAAGAGAAAGTGCATTACAACTGTAAAAGGATTAGACCTTTTTGGAGTTAAGCTAAGTGATGCTTCAAAGAAACTTGGGAAAAAGTTTGCTACTGGTGCTTCTGTGGTTAAGGGGCCAACTGAGAAAGAGCAAATTGATGTTCAAGGTGACATAGCCTATGACATCGTGGATTTCATTACGGAGACTTGGCCTGATCTAGTTTTTGGGGTGTGGTTCTCCAAGGTGCTTAAGTACCTAGCAATGGGATGGGGTATTGTTAGGAACCAAGGTTGTATGGGATGTCTTTGTCCCACATTGGTTAGAATGGGAGGACCAATGTGGTACTTGAGTAGCTTGGCTCTCCCACCCCAACAACTGCTTTTGGAGTGTGGTTCTCCAATGTGCTTAAGTACATAA

Protein sequence

MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVSNQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKGLDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDLVFGVWFSKVLKYLAMGWGIVRNQGCMGCLCPTLVRMGGPMWYLSSLALPPQQLLLECGSPMCLST*
Homology
BLAST of CSPI01G28240 vs. ExPASy Swiss-Prot
Match: O42929 (Translation machinery-associated protein 22 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tma22 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.0e-27
Identity = 74/174 (42.53%), Postives = 104/174 (59.77%), Query Frame = 0

Query: 5   PQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVSNQLQ 64
           P+   VLYC VC+LP EYCEF    +KCK WL  + PD+Y  L  E       ++S  L+
Sbjct: 7   PKLKSVLYCDVCTLPVEYCEFEGTLKKCKEWLKSSHPDVYDKLYGEQ------DLSKDLE 66

Query: 65  STSISSAAGDGAASSGPKQ--EEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKGLD 124
           +T   S   D  A   P +  +E KR+   + K+    +V+I+ + R KRK +TTV+GLD
Sbjct: 67  NTLNVSGTKDSNAEEQPAKLTKEEKRVEREEAKRM-ASKVLIKTIERTKRKRVTTVQGLD 126

Query: 125 LFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
            FG++   A+K L  KFATGASV K   +K++I VQGD+ YDI DFI E + ++
Sbjct: 127 AFGIETKKAAKMLANKFATGASVTKTADKKDEIVVQGDLNYDIFDFILEKFKEV 173

BLAST of CSPI01G28240 vs. ExPASy Swiss-Prot
Match: Q2H5Z7 (Translation machinery-associated protein 22 OS=Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970) OX=306901 GN=TMA22 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.5e-26
Identity = 73/178 (41.01%), Postives = 105/178 (58.99%), Query Frame = 0

Query: 1   MAEKPQPV--RVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGE 60
           MAE P PV   ++YC VC+LP EYCE+G   +KC+ WL +  PDLY  +     + EA E
Sbjct: 1   MAE-PTPVGREIIYCGVCTLPPEYCEYGGTTKKCQEWLEKKHPDLYARIW----SPEALE 60

Query: 61  VSNQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTV 120
            +    S +    A   AA    K E  ++    K+    K  V I+++ RNKRK +T+V
Sbjct: 61  AATASLSLAAQERAAKDAAKKAAKAEAAEQKHADKLA---KSVVTIKRIERNKRKFVTSV 120

Query: 121 KGLDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
            GL+ FG++L   +K  GKKFATGASV K P+  E+I VQGD++ +I +F+ E + D+
Sbjct: 121 TGLEAFGLELKKVAKDFGKKFATGASVTKVPSGGEEIVVQGDVSGEIEEFLLEKYKDI 170

BLAST of CSPI01G28240 vs. ExPASy Swiss-Prot
Match: Q6GLZ1 (Density-regulated protein OS=Xenopus laevis OX=8355 GN=denr PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 7.4e-26
Identity = 72/176 (40.91%), Postives = 104/176 (59.09%), Query Frame = 0

Query: 7   PVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDL-LKEANAKEAGEVSNQLQS 66
           P++VLYC VCSLP EYCE+ PD  KC+ WL +N PD++  L L     +E G V      
Sbjct: 28  PLKVLYCGVCSLPTEYCEYMPDVAKCRQWLEKNFPDVFAKLTLGHIPKQETGTV------ 87

Query: 67  TSISSAAGDGAASSGPKQEEVKRLPGGK--IKKKDK---QEVIIEKVTRNKRKCITTVKG 126
                   +G A+SG ++E+ K+  GG+  IK+K K   Q + I K+ R K+K +T V G
Sbjct: 88  --------EGQATSGEEEEKKKQKRGGRGQIKQKKKTVPQRITIAKIPRAKKKYVTRVCG 147

Query: 127 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           L  F ++L DA +   +KF+ GASV    T +++I +QGD   DI+D I E WP++
Sbjct: 148 LATFEIELKDAQRFFAQKFSCGASV----TGEDEIIIQGDFTDDIIDVIQEKWPEV 185

BLAST of CSPI01G28240 vs. ExPASy Swiss-Prot
Match: Q5ZJ39 (Density-regulated protein OS=Gallus gallus OX=9031 GN=DENR PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 9.6e-26
Identity = 70/175 (40.00%), Postives = 103/175 (58.86%), Query Frame = 0

Query: 7   PVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVSNQLQST 66
           P+RVLYC VCSLP EYCE+ PD  KC+ WL +N PD +  L  E + K+           
Sbjct: 28  PLRVLYCGVCSLPTEYCEYMPDVTKCRQWLEKNFPDEFAKLTVENSPKQ----------- 87

Query: 67  SISSAAGDGAASSGPKQEEVKRLPGGK--IKKKDK---QEVIIEKVTRNKRKCITTVKGL 126
              +  G+G  ++G ++E+ K+  GG+  IK+K K   Q+V I K+ R K+K +T V GL
Sbjct: 88  --EAGVGEGQGTAGEEEEKKKQKRGGRGQIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGL 147

Query: 127 DLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
             F + L +A +   +KF+ GASV    T +++I +QGD   DI+D I E WP++
Sbjct: 148 ATFEIDLKEAQRFFAQKFSCGASV----TGEDEIIIQGDFTDDIIDVIQEKWPEV 185

BLAST of CSPI01G28240 vs. ExPASy Swiss-Prot
Match: Q5RFP5 (Density-regulated protein OS=Pongo abelii OX=9601 GN=DENR PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.3e-25
Identity = 70/174 (40.23%), Postives = 101/174 (58.05%), Query Frame = 0

Query: 7   PVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVSNQLQST 66
           P+RVLYC VCSLP EYCE+ PD  KC+ WL +N P+ +  L  E + K+   +S      
Sbjct: 28  PLRVLYCGVCSLPTEYCEYMPDVAKCRQWLEKNFPNEFAKLTVENSPKQEAGISE----- 87

Query: 67  SISSAAGDGAASSGPKQEEVKRLPGGKIKKKDK---QEVIIEKVTRNKRKCITTVKGLDL 126
                 G G A    ++++ KR   G+IK+K K   Q+V I K+ R K+K +T V GL  
Sbjct: 88  ------GQGTAGEEEEKKKQKRGGRGQIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGLAT 147

Query: 127 FGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDLV 178
           F + L +A +   +KF+ GASV    T +++I +QGD   DI+D I E WP++V
Sbjct: 148 FEIDLKEAQRFFAQKFSCGASV----TGEDEIIIQGDFTDDIIDVIQEKWPEVV 186

BLAST of CSPI01G28240 vs. ExPASy TrEMBL
Match: A0A5A7UNG3 (Translation machinery-associated protein 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G002210 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 9.1e-104
Identity = 201/238 (84.45%), Postives = 210/238 (88.24%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEV+IEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVVIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDLVFGV 180
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPD+   V
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDV---V 180

Query: 181 WFSKVLKYLAMGWGIVRNQGC--------MGCLCPTLVRMGGPMWYLSSLALPPQQLL 231
           W + V  +L      V+            + CLCPTLVR+G P+WYLSSLALPPQ +L
Sbjct: 181 WDASVPHWLECNDQCVKPPESFDRVWPSEVPCLCPTLVRIGEPVWYLSSLALPPQWML 235

BLAST of CSPI01G28240 vs. ExPASy TrEMBL
Match: A0A0A0LWI1 (Translation machinery-associated protein 22 OS=Cucumis sativus OX=3659 GN=Csa_1G589080 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.1e-92
Identity = 175/176 (99.43%), Postives = 176/176 (100.00%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPD+
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDV 176

BLAST of CSPI01G28240 vs. ExPASy TrEMBL
Match: A0A6J1H953 (Translation machinery-associated protein 22 OS=Cucurbita moschata OX=3662 GN=LOC111461628 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.1e-88
Identity = 168/176 (95.45%), Postives = 173/176 (98.30%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MA+KPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MADKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGA SS PKQEEVKRLPGGKIKKK++QEVIIEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGATSSAPKQEEVKRLPGGKIKKKERQEVIIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDI+YDIVDFITETW D+
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDISYDIVDFITETWRDV 176

BLAST of CSPI01G28240 vs. ExPASy TrEMBL
Match: A0A6J1JK26 (Translation machinery-associated protein 22 OS=Cucurbita maxima OX=3661 GN=LOC111485287 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.4e-88
Identity = 167/176 (94.89%), Postives = 173/176 (98.30%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MA+KPQPVRVLYCP+CSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MADKPQPVRVLYCPICSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGA SS PKQEEVKRLPGGKIKKK++QEVIIEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGATSSAPKQEEVKRLPGGKIKKKERQEVIIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDI+YDIVDFITETW D+
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDISYDIVDFITETWRDV 176

BLAST of CSPI01G28240 vs. ExPASy TrEMBL
Match: A0A6J1D0S6 (Translation machinery-associated protein 22 OS=Momordica charantia OX=3673 GN=LOC111016533 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.8e-88
Identity = 167/176 (94.89%), Postives = 172/176 (97.73%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MAEKPQPV VLYCPVCSLPAEYCEFGPDFEKCKPWLI+NAPDLYPDLLKEANAKEA E S
Sbjct: 1   MAEKPQPVLVLYCPVCSLPAEYCEFGPDFEKCKPWLIRNAPDLYPDLLKEANAKEAAEAS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGAASS PKQEEVKRLPGGKIKKK+KQEV+IEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGAASSAPKQEEVKRLPGGKIKKKEKQEVVIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDF+TETWPD+
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFLTETWPDV 176

BLAST of CSPI01G28240 vs. NCBI nr
Match: KAA0055826.1 (translation machinery-associated protein 22 [Cucumis melo var. makuwa])

HSP 1 Score: 386.3 bits (991), Expect = 1.9e-103
Identity = 201/238 (84.45%), Postives = 210/238 (88.24%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEV+IEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVVIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDLVFGV 180
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPD+   V
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDV---V 180

Query: 181 WFSKVLKYLAMGWGIVRNQGC--------MGCLCPTLVRMGGPMWYLSSLALPPQQLL 231
           W + V  +L      V+            + CLCPTLVR+G P+WYLSSLALPPQ +L
Sbjct: 181 WDASVPHWLECNDQCVKPPESFDRVWPSEVPCLCPTLVRIGEPVWYLSSLALPPQWML 235

BLAST of CSPI01G28240 vs. NCBI nr
Match: XP_004144004.1 (translation machinery-associated protein 22 [Cucumis sativus] >KAE8653433.1 hypothetical protein Csa_007666 [Cucumis sativus])

HSP 1 Score: 348.6 bits (893), Expect = 4.4e-92
Identity = 175/176 (99.43%), Postives = 176/176 (100.00%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPD+
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDV 176

BLAST of CSPI01G28240 vs. NCBI nr
Match: XP_038879416.1 (translation machinery-associated protein 22 [Benincasa hispida])

HSP 1 Score: 342.0 bits (876), Expect = 4.1e-90
Identity = 172/176 (97.73%), Postives = 174/176 (98.86%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGAASS  KQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGAASSASKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDI+YDIVDFITETWPD+
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDISYDIVDFITETWPDV 176

BLAST of CSPI01G28240 vs. NCBI nr
Match: KAG6590002.1 (hypothetical protein SDJN03_15425, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 336.3 bits (861), Expect = 2.2e-88
Identity = 168/176 (95.45%), Postives = 173/176 (98.30%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MA+KPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MADKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGA SS PKQEEVKRLPGGKIKKK++QEVIIEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGATSSAPKQEEVKRLPGGKIKKKERQEVIIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDI+YDIVDFITETW D+
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDISYDIVDFITETWRDV 176

BLAST of CSPI01G28240 vs. NCBI nr
Match: XP_022960996.1 (translation machinery-associated protein 22-like [Cucurbita moschata] >KAG7023664.1 tma22 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 336.3 bits (861), Expect = 2.2e-88
Identity = 168/176 (95.45%), Postives = 173/176 (98.30%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MA+KPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS
Sbjct: 1   MADKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60

Query: 61  NQLQSTSISSAAGDGAASSGPKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCITTVKG 120
           NQLQSTSISSAAGDGA SS PKQEEVKRLPGGKIKKK++QEVIIEKVTRNKRKCITTVKG
Sbjct: 61  NQLQSTSISSAAGDGATSSAPKQEEVKRLPGGKIKKKERQEVIIEKVTRNKRKCITTVKG 120

Query: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPDL 177
           LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDI+YDIVDFITETW D+
Sbjct: 121 LDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDISYDIVDFITETWRDV 176

BLAST of CSPI01G28240 vs. TAIR 10
Match: AT5G11900.1 (Translation initiation factor SUI1 family protein )

HSP 1 Score: 285.4 bits (729), Expect = 4.2e-77
Identity = 143/181 (79.01%), Postives = 158/181 (87.29%), Query Frame = 0

Query: 1   MAEKPQPVRVLYCPVCSLPAEYCEFGPDFEKCKPWLIQNAPDLYPDLLKEANAKEAGEVS 60
           MAEK +PV+VLYC VCSLPAEYCEFGPDF +CKPWL++NAPDLYPDLLKEAN K A  VS
Sbjct: 1   MAEKLEPVKVLYCGVCSLPAEYCEFGPDFARCKPWLVENAPDLYPDLLKEANEKAADNVS 60

Query: 61  NQLQSTSISSAAGDGAASSG-----PKQEEVKRLPGGKIKKKDKQEVIIEKVTRNKRKCI 120
           ++LQS  ISS   DGA SS       K+EEVKRLPGGK+KKKD+QEVIIEKV RNKRKCI
Sbjct: 61  DKLQSVGISSGGADGAPSSAQTGGTSKKEEVKRLPGGKVKKKDRQEVIIEKVVRNKRKCI 120

Query: 121 TTVKGLDLFGVKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIAYDIVDFITETWPD 177
           T VKGL+LFG+KLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDI YDIV+FIT+TWPD
Sbjct: 121 TIVKGLELFGIKLSDASKKLGKKFATGASVVKGPTEKEQIDVQGDIIYDIVEFITDTWPD 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O429293.0e-2742.53Translation machinery-associated protein 22 OS=Schizosaccharomyces pombe (strain... [more]
Q2H5Z72.5e-2641.01Translation machinery-associated protein 22 OS=Chaetomium globosum (strain ATCC ... [more]
Q6GLZ17.4e-2640.91Density-regulated protein OS=Xenopus laevis OX=8355 GN=denr PE=2 SV=1[more]
Q5ZJ399.6e-2640.00Density-regulated protein OS=Gallus gallus OX=9031 GN=DENR PE=2 SV=1[more]
Q5RFP51.3e-2540.23Density-regulated protein OS=Pongo abelii OX=9601 GN=DENR PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UNG39.1e-10484.45Translation machinery-associated protein 22 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A0A0LWI12.1e-9299.43Translation machinery-associated protein 22 OS=Cucumis sativus OX=3659 GN=Csa_1G... [more]
A0A6J1H9531.1e-8895.45Translation machinery-associated protein 22 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JK261.4e-8894.89Translation machinery-associated protein 22 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1D0S61.8e-8894.89Translation machinery-associated protein 22 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
KAA0055826.11.9e-10384.45translation machinery-associated protein 22 [Cucumis melo var. makuwa][more]
XP_004144004.14.4e-9299.43translation machinery-associated protein 22 [Cucumis sativus] >KAE8653433.1 hypo... [more]
XP_038879416.14.1e-9097.73translation machinery-associated protein 22 [Benincasa hispida][more]
KAG6590002.12.2e-8895.45hypothetical protein SDJN03_15425, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022960996.12.2e-8895.45translation machinery-associated protein 22-like [Cucurbita moschata] >KAG702366... [more]
Match NameE-valueIdentityDescription
AT5G11900.14.2e-7779.01Translation initiation factor SUI1 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001950SUI1 domainPFAMPF01253SUI1coord: 99..171
e-value: 1.9E-19
score: 69.9
IPR001950SUI1 domainPROSITEPS50296SUI1coord: 102..173
score: 19.364788
NoneNo IPR availableGENE3D3.30.780.10coord: 52..176
e-value: 5.6E-19
score: 70.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..91
NoneNo IPR availablePANTHERPTHR12789:SF3TRANSLATION MACHINERY-ASSOCIATED PROTEIN 22coord: 1..176
NoneNo IPR availablePANTHERPTHR12789DENSITY-REGULATED PROTEIN HOMOLOGcoord: 1..176
NoneNo IPR availableCDDcd11607DENR_Ccoord: 100..176
e-value: 3.76243E-42
score: 136.533
IPR005873DENR family, eukaryotesTIGRFAMTIGR01159TIGR01159coord: 7..176
e-value: 2.3E-52
score: 175.3
IPR036877SUI1 domain superfamilySUPERFAMILY55159eIF1-likecoord: 19..174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G28240.1CSPI01G28240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001731 formation of translation preinitiation complex
biological_process GO:0002188 translation reinitiation
biological_process GO:0006413 translational initiation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005840 ribosome
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003743 translation initiation factor activity