CSPI01G28220 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G28220
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytokinin dehydrogenase
LocationChr1: 22956155 .. 22958633 (-)
RNA-Seq ExpressionCSPI01G28220
SyntenyCSPI01G28220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGCTCCTAACCCTTTTCTTCTCATAATTTTCACTTGCTTTCTTTTCCTTCAAAACGCACCGTTTTTTGGAGTGGCTTTCAGTGTAGCTTTTCCAAAAGCAATTTCAGATAAGCTTAAATATGATCTTCAAACCATTAGCTTAGCTTCCAAAGATTATGGCTTGATGGTTCAACACAATCCTTATGCCGTTTTCTTTCCTGTGACTGGTCACGACGTCGCCGGCTTGATCCGCTTCATGTATATGCTTCCGGTCCCCTTGCATATCGCTGCTCGAGGTCAAGGCCATTGTGTTCGAGGCCAAGCGTTGGTTGATCAGAATGGTGTTGTTGTAAACATGACGTCCCTTGGTGGTTTACGACAAAAGACAAGTAGAATTGTGGTGACGACGACGTCGCCGTTGGGTCCGTATGCCGACGTTGGTGCTGAGCAACTTTGGATCGATGTACTACATGCAACCACACAGAAGGGTTTAAGCCCTGTCTCGTGGGTGGATTATTTGCATATTACCGTTGGCGGGACGTTGTCCAATGCTGGAATTAGCGGCCAAACGTTTCGGTTCGGTCCTCAGATCAACAATGTTTATGAACTTGATGTCATAACTGGTACGTATTTCTTCAATGACTCTTTTACATTTTTCTACATCTTACTTATTACGCTCCATTTTTTTTCATTTTGAACATATAACAACCTACCAAAAGCAAAAACTTTTAATTACTTTCATACACATCCTATCATTACTACCATGTATGGAAAGGTAATAAGAAAAACCAAACCTCATAATTGTGAATAAAATAAGTTTTAGAATTGTCTTTTGGTCATCATTTGTGGGTGTTGGGATTATTGTATGAAAAAGGACAAAGAATAAGTTAGATGAATAATTAAAATGTGTAAGGCTCTTTGGAGATGTCGTCGTATATAGACTATGCAGTTATTGTCTTTTGACGATAATTTCAGTAAGTGTACGTAAGACTTAGTTGTTGCAAGCTTAGACATCATTTATCTCCATTCATTTTTTTTCAAATACAAAATGAAATTACACATAGATCGTTTTCTATATGTACAACTCAGCCCTGTTACACCAAAGTATACACAAACACACCACTTTCCATATGTTATTAACCTCTTTTTTCTCCAAACCCAAACCTCAATCAAATTCAGCTTTTTCCACGACAATGACATTGATAATTATTTTTATTGAAAAAGTTTATAACCCTAATAACAATCCTTTTTTGTTTAAATGACAGGAAAAGGAGAATCCTTGACTTGCTCTCCCACCAACAATCCCGAACTCTTTTACTCCCTCCTCGGCGGTTTGGGTCAGTTCGGCATAATAACCCGAGCTAGAATCGCTCTTGCTCCAACTCCCACCAGGGTCTCTCTCTCTCTCTCTTTCTCTCTTTAGTTTAATTCATTACCTTATAATAATAAAACACAAATGAATTGAGATTTCACTAAACAGGCCTTAGCCGGCTAATCATTATTCAATTCAATGACGTTTTTCTAAAATCAAATATTTATTTACCACTTTTTAAAATTTATAACTTTCCTTTAACCCTATTTTTTATTTTCCATTTTTTTCATAATATAATACTAATAATTTCTTTTGACTTGAAAAAAAATACATTTGAAATATATATAATGATTTTAATTAACGACCTTACGTGCACTTAACGGTTTCTTTGACTTTTAGAATTGATATTATTTTTTAATCTTTTTTTTTTTTTTTTTTTGGGGACAACATTTTGATTAAATTAAGGTTAATAAGATTTTATTTGCTGACATGGATTTTTTTTGTTTATTTAAATGTATAAATTTGCTTAAGGTTAAATGGGTTCGGATGCTTTACACCAACTTCTCTTCCTTTACAAGTGATCAAGAGAGTTTGATATCTAGAGATCAATCCAACGCCCCAGATTACCTTGAAGGATTGCTTTTGCTACAACTCAATGCAGGTGATAAGTCTTCGTTCTACCCTCTCCCAGACCAACCCAAGATTTCATCCCTTGTTTCTCAATACGGCATCGTTTACGTCCTTGAACTTGTCAAATATTATGATCAACATTCTTCTTCTTCTGTTGACCAGGTTCGTTCTATTGATTATTCATTCCTTCGTGTTTGAGTAATTTTGTTTCAATTCGGTTTTTAAAATGTTTTTTTTTTTCTAATAATGACATGTGCAGGAATTGGAGACTTTGTTGGGGGGGTTGAAGTTTGAGGCAGGGATGAAGTTTGTTAAGGATGCTTCTTACGAAGAGTTTTTAGATCGAGTTCATACCGACGAGGTAGCTTTAAGAGCTTTAGGGTTATGGGAAGTTCCTCATCCGTGGATAAATCTTTTCGTTCCGAAGTCGAGAATTGCCGATTTTGATTCTGGTGTTTTTAGAGGCATTATTCAAAAGCGTAATCTCACTTCTGGCGTCTTCCTCTTCTACCCCATGTTCAAAAACAAGTACGTGTTCTCTTTTTTTTTTTAA

mRNA sequence

ATGGATGCTCCTAACCCTTTTCTTCTCATAATTTTCACTTGCTTTCTTTTCCTTCAAAACGCACCGTTTTTTGGAGTGGCTTTCAGTGTAGCTTTTCCAAAAGCAATTTCAGATAAGCTTAAATATGATCTTCAAACCATTAGCTTAGCTTCCAAAGATTATGGCTTGATGGTTCAACACAATCCTTATGCCGTTTTCTTTCCTGTGACTGGTCACGACGTCGCCGGCTTGATCCGCTTCATGTATATGCTTCCGGTCCCCTTGCATATCGCTGCTCGAGGTCAAGGCCATTGTGTTCGAGGCCAAGCGTTGGTTGATCAGAATGGTGTTGTTGTAAACATGACGTCCCTTGGTGGTTTACGACAAAAGACAAGTAGAATTGTGGTGACGACGACGTCGCCGTTGGGTCCGTATGCCGACGTTGGTGCTGAGCAACTTTGGATCGATGTACTACATGCAACCACACAGAAGGGTTTAAGCCCTGTCTCGTGGGTGGATTATTTGCATATTACCGTTGGCGGGACGTTGTCCAATGCTGGAATTAGCGGCCAAACGTTTCGGTTCGGTCCTCAGATCAACAATGTTTATGAACTTGATGTCATAACTGGAAAAGGAGAATCCTTGACTTGCTCTCCCACCAACAATCCCGAACTCTTTTACTCCCTCCTCGGCGGTTTGGGTCAGTTCGGCATAATAACCCGAGCTAGAATCGCTCTTGCTCCAACTCCCACCAGGGTTAAATGGGTTCGGATGCTTTACACCAACTTCTCTTCCTTTACAAGTGATCAAGAGAGTTTGATATCTAGAGATCAATCCAACGCCCCAGATTACCTTGAAGGATTGCTTTTGCTACAACTCAATGCAGGTGATAAGTCTTCGTTCTACCCTCTCCCAGACCAACCCAAGATTTCATCCCTTGTTTCTCAATACGGCATCGTTTACGTCCTTGAACTTGTCAAATATTATGATCAACATTCTTCTTCTTCTGTTGACCAGGAATTGGAGACTTTGTTGGGGGGGTTGAAGTTTGAGGCAGGGATGAAGTTTGTTAAGGATGCTTCTTACGAAGAGTTTTTAGATCGAGTTCATACCGACGAGGTAGCTTTAAGAGCTTTAGGGTTATGGGAAGTTCCTCATCCGTGGATAAATCTTTTCGTTCCGAAGTCGAGAATTGCCGATTTTGATTCTGGTGTTTTTAGAGGCATTATTCAAAAGCGTAATCTCACTTCTGGCGTCTTCCTCTTCTACCCCATGTTCAAAAACAAGTACGTGTTCTCTTTTTTTTTTTAA

Coding sequence (CDS)

ATGGATGCTCCTAACCCTTTTCTTCTCATAATTTTCACTTGCTTTCTTTTCCTTCAAAACGCACCGTTTTTTGGAGTGGCTTTCAGTGTAGCTTTTCCAAAAGCAATTTCAGATAAGCTTAAATATGATCTTCAAACCATTAGCTTAGCTTCCAAAGATTATGGCTTGATGGTTCAACACAATCCTTATGCCGTTTTCTTTCCTGTGACTGGTCACGACGTCGCCGGCTTGATCCGCTTCATGTATATGCTTCCGGTCCCCTTGCATATCGCTGCTCGAGGTCAAGGCCATTGTGTTCGAGGCCAAGCGTTGGTTGATCAGAATGGTGTTGTTGTAAACATGACGTCCCTTGGTGGTTTACGACAAAAGACAAGTAGAATTGTGGTGACGACGACGTCGCCGTTGGGTCCGTATGCCGACGTTGGTGCTGAGCAACTTTGGATCGATGTACTACATGCAACCACACAGAAGGGTTTAAGCCCTGTCTCGTGGGTGGATTATTTGCATATTACCGTTGGCGGGACGTTGTCCAATGCTGGAATTAGCGGCCAAACGTTTCGGTTCGGTCCTCAGATCAACAATGTTTATGAACTTGATGTCATAACTGGAAAAGGAGAATCCTTGACTTGCTCTCCCACCAACAATCCCGAACTCTTTTACTCCCTCCTCGGCGGTTTGGGTCAGTTCGGCATAATAACCCGAGCTAGAATCGCTCTTGCTCCAACTCCCACCAGGGTTAAATGGGTTCGGATGCTTTACACCAACTTCTCTTCCTTTACAAGTGATCAAGAGAGTTTGATATCTAGAGATCAATCCAACGCCCCAGATTACCTTGAAGGATTGCTTTTGCTACAACTCAATGCAGGTGATAAGTCTTCGTTCTACCCTCTCCCAGACCAACCCAAGATTTCATCCCTTGTTTCTCAATACGGCATCGTTTACGTCCTTGAACTTGTCAAATATTATGATCAACATTCTTCTTCTTCTGTTGACCAGGAATTGGAGACTTTGTTGGGGGGGTTGAAGTTTGAGGCAGGGATGAAGTTTGTTAAGGATGCTTCTTACGAAGAGTTTTTAGATCGAGTTCATACCGACGAGGTAGCTTTAAGAGCTTTAGGGTTATGGGAAGTTCCTCATCCGTGGATAAATCTTTTCGTTCCGAAGTCGAGAATTGCCGATTTTGATTCTGGTGTTTTTAGAGGCATTATTCAAAAGCGTAATCTCACTTCTGGCGTCTTCCTCTTCTACCCCATGTTCAAAAACAAGTACGTGTTCTCTTTTTTTTTTTAA

Protein sequence

MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQESLISRDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQPKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNKYVFSFFF*
Homology
[Close]
BLAST of CSPI01G28220 vs. ExPASy Swiss-Prot
Match: Q9FUJ2 (Cytokinin dehydrogenase 4 OS=Arabidopsis thaliana OX=3702 GN=CKX4 PE=1 SV=2)

HSP 1 Score: 398.7 bits (1023), Expect = 8.5e-110
Identity = 209/404 (51.73%), Postives = 271/404 (67.08%), Query Frame = 0 

Query: 30  VAFPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRF--------- 89
           V  P +++  +  D  +IS AS D+G +   NP AV  P +  +VA L+RF         
Sbjct: 32  VFLPISLNLTVLTDPFSISAASHDFGNITDENPGAVLCPSSTTEVARLLRFANGGFSYNK 91

Query: 90  -MYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYA 149
                     +AARGQGH +RGQA     GVVVNMT L  +  K + +V++     G YA
Sbjct: 92  GSTSPASTFKVAARGQGHSLRGQASA-PGGVVVNMTCL-AMAAKPAAVVISAD---GTYA 151

Query: 150 DVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELD 209
           DV A  +W+DVL A   +G+SPV+W DYL+++VGGTLSNAGI GQTFR GPQI+NV+ELD
Sbjct: 152 DVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVGGTLSNAGIGGQTFRHGPQISNVHELD 211

Query: 210 VITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSF 269
           VITGKGE +TCSP  NPELFY +LGGLGQFGIITRARIAL   PTRVKW R+LY++FS+F
Sbjct: 212 VITGKGEMMTCSPKLNPELFYGVLGGLGQFGIITRARIALDHAPTRVKWSRILYSDFSAF 271

Query: 270 TSDQESLISRDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQPKISSLVSQYGIVYVLELV 329
             DQE LIS       D+LEG L++  N    +SF+PL DQ +++SLV+ + I+YVLE+ 
Sbjct: 272 KRDQERLISMTNDLGVDFLEGQLMMS-NGFVDTSFFPLSDQTRVASLVNDHRIIYVLEVA 331

Query: 330 KYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPH 389
           KYYD+ +   +DQ ++TL   L F  G  FV+D  Y +FL+RV  +E  LR+LGLWEVPH
Sbjct: 332 KYYDRTTLPIIDQVIDTLSRTLGFAPGFMFVQDVPYFDFLNRVRNEEDKLRSLGLWEVPH 391

Query: 390 PWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           PW+N+FVP SRI DF  GV  G++  +  TSGV LFYP  +NK+
Sbjct: 392 PWLNIFVPGSRIQDFHDGVINGLLLNQTSTSGVTLFYPTNRNKW 429

[Close]
BLAST of CSPI01G28220 vs. ExPASy Swiss-Prot
Match: Q6YW50 (Cytokinin dehydrogenase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX7 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 4.9e-105
Identity = 198/398 (49.75%), Postives = 264/398 (66.33%), Query Frame = 0 

Query: 32  FPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRFMYMLPVPLHIA 91
           F  +++ KL+ D  + + AS D+G +V   P AV  P T  ++A L+RF    P P  +A
Sbjct: 29  FALSVASKLRVDRNSTARASSDFGRIVAAAPEAVLHPATPAEIAELVRFSASSPSPFPVA 88

Query: 92  ARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQLWIDVL 151
            RGQGH  RGQ+L    GVVV+M +L   R +     V  ++   PY D G EQLW DVL
Sbjct: 89  PRGQGHSARGQSLA-PGGVVVDMRALASRRGR-----VNVSAGAAPYVDAGGEQLWADVL 148

Query: 152 HATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKGESLTCS 211
            AT + GL+P  W DYL ITV GTLSNAGI GQ FR GPQI NV ELDVITG G+ +TCS
Sbjct: 149 RATLEHGLAPRVWTDYLRITVAGTLSNAGIGGQAFRHGPQIANVLELDVITGTGDMVTCS 208

Query: 212 PTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQESLISRDQ 271
              + +LF+++LGGLGQFGIITRARI L P P RV+WVR+ Y++ ++FT DQE LIS+  
Sbjct: 209 RDKDSDLFFAVLGGLGQFGIITRARIGLMPAPKRVRWVRLAYSDVATFTKDQELLISKRA 268

Query: 272 SNAP-DYLEGLLLLQ--LNAGDKSS-FYPLPDQPKISSLVSQ--YGIVYVLELVKYYDQH 331
           S A  DY+EG + L   L  G KS+ F+   D  +++ L S+   G++YV+E   YY++ 
Sbjct: 269 SEAGFDYVEGQVQLNRTLTEGPKSTPFFSSSDIGRLAGLASKSVSGVIYVIEGTMYYNES 328

Query: 332 SSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLF 391
           +S+++DQ+LE++LG L FE G  F KD  Y +FLDRV  +E  LR++G+W+VPHPW+NLF
Sbjct: 329 TSTTMDQKLESILGQLSFEEGFVFTKDVRYVQFLDRVREEERVLRSIGMWDVPHPWLNLF 388

Query: 392 VPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           VP+SRI DFD+GVF+G+    N   GV L YPM  N +
Sbjct: 389 VPRSRILDFDAGVFKGVFAGAN-PVGVILMYPMNTNMW 419

[Close]
BLAST of CSPI01G28220 vs. ExPASy Swiss-Prot
Match: Q9FUJ3 (Cytokinin dehydrogenase 2 OS=Arabidopsis thaliana OX=3702 GN=CKX2 PE=1 SV=2)

HSP 1 Score: 382.5 bits (981), Expect = 6.3e-105
Identity = 198/394 (50.25%), Postives = 257/394 (65.23%), Query Frame = 0 

Query: 30  VAFPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRFMYMLPVPLH 89
           +  PK+++  L  D   IS AS D+G +    P  V  P +  D++ L+++         
Sbjct: 25  IDLPKSLNLTLSTDPSIISAASHDFGNITTVTPGGVICPSSTADISRLLQYAANGKSTFQ 84

Query: 90  IAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQLWID 149
           +AARGQGH + GQA V   GV+VNMT +       + +VV+       YADV A  LW+D
Sbjct: 85  VAARGQGHSLNGQASV-SGGVIVNMTCI-------TDVVVSKDK---KYADVAAGTLWVD 144

Query: 150 VLHATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKGESLT 209
           VL  T +KG+SPVSW DYLHITVGGTLSN GI GQ FR GP ++NV ELDVITGKGE LT
Sbjct: 145 VLKKTAEKGVSPVSWTDYLHITVGGTLSNGGIGGQVFRNGPLVSNVLELDVITGKGEMLT 204

Query: 210 CSPTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQESLISR 269
           CS   NPELFY +LGGLGQFGIITRARI L   P R KW RMLY++F++FT DQE LIS 
Sbjct: 205 CSRQLNPELFYGVLGGLGQFGIITRARIVLDHAPKRAKWFRMLYSDFTTFTKDQERLISM 264

Query: 270 DQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQPKISSLVSQYGIVYVLELVKYYDQHSSSS 329
                 DYLEG + L     D +SF+P  DQ K++ LV Q+GI+YVLE+ KYYD  +   
Sbjct: 265 ANDIGVDYLEGQIFLSNGVVD-TSFFPPSDQSKVADLVKQHGIIYVLEVAKYYDDPNLPI 324

Query: 330 VDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLFVPKS 389
           + + ++TL   L +  G   + D +Y +FL+RVH +E  LR+LGLWE+PHPW+NL+VPKS
Sbjct: 325 ISKVIDTLTKTLSYLPGFISMHDVAYFDFLNRVHVEENKLRSLGLWELPHPWLNLYVPKS 384

Query: 390 RIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           RI DF +GV + I+ K+   SG+ L YP  +NK+
Sbjct: 385 RILDFHNGVVKDILLKQKSASGLALLYPTNRNKW 406

[Close]
BLAST of CSPI01G28220 vs. ExPASy Swiss-Prot
Match: Q6YW51 (Cytokinin dehydrogenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX6 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 2.7e-103
Identity = 197/398 (49.50%), Postives = 262/398 (65.83%), Query Frame = 0 

Query: 32  FPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRFMYMLPVPLHIA 91
           F  +++ KL+ D  T + AS D+G +V   P AV  P T  ++A L+RF    P P  +A
Sbjct: 29  FAHSVASKLRVDRDTTARASSDFGRIVAAAPEAVLHPATPAEIAELVRFSASSPSPFPVA 88

Query: 92  ARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQLWIDVL 151
            RGQGH  RGQ+L    GVVV+M +L   R + +  V    +   PY D G EQLW DVL
Sbjct: 89  PRGQGHSARGQSLA-PGGVVVDMRALAARRGRVN--VSAGGAGAAPYVDAGGEQLWADVL 148

Query: 152 HATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKGESLTCS 211
            AT + GL+P  W DYL ITV GTLSNAGI GQ FR GPQI NV ELDVITG+G+ +TCS
Sbjct: 149 RATLEHGLAPRVWTDYLRITVAGTLSNAGIGGQAFRHGPQIANVLELDVITGRGDMVTCS 208

Query: 212 PTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQESLISRDQ 271
               P+LF+++LGGLGQFGIITRARI L P P RV+WVR+ Y++  +FT DQE LIS+  
Sbjct: 209 RDKEPDLFFAVLGGLGQFGIITRARIGLEPAPKRVRWVRLAYSDVVTFTRDQELLISKRA 268

Query: 272 SNAP-DYLEGLLLLQ--LNAGDKSS-FYPLPDQPKISSLVSQ--YGIVYVLELVKYYDQH 331
           S A  DY+EG + L   L  G KS+ F+   D  +++ L S+   G++Y +E   YY++ 
Sbjct: 269 SEAGFDYVEGQVQLNRTLTEGPKSTPFFSRFDIDRLAGLASESVSGVIYFIEGAMYYNES 328

Query: 332 SSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLF 391
           +++SVDQ+L ++L  L F+ G  F KD SY +FLDRV  +E  LR++G+W+VPHPW+NLF
Sbjct: 329 TTASVDQKLTSVLEQLSFDKGFVFTKDVSYVQFLDRVREEERILRSIGMWDVPHPWLNLF 388

Query: 392 VPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           VP+SRI DFD+GV +G+    N   GV L YPM +N +
Sbjct: 389 VPQSRILDFDTGVLKGVFVGAN-PVGVILMYPMNRNMW 422

[Close]
BLAST of CSPI01G28220 vs. ExPASy Swiss-Prot
Match: Q9LTS3 (Cytokinin dehydrogenase 3 OS=Arabidopsis thaliana OX=3702 GN=CKX3 PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 3.3e-101
Identity = 188/387 (48.58%), Postives = 261/387 (67.44%), Query Frame = 0 

Query: 39  KLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHC 98
           KL     ++  A+ D+G + +  P AV  P +  D+  LI+  +   +   +AARG GH 
Sbjct: 47  KLTSSSSSVESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFDSQLSFPLAARGHGHS 106

Query: 99  VRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKG 158
            RGQA   ++GVVVNM S+   R +  ++  T       Y DV A  LWI+VL+ T + G
Sbjct: 107 HRGQASA-KDGVVVNMRSMVN-RDRGIKVSRTCL-----YVDVDAAWLWIEVLNKTLELG 166

Query: 159 LSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPEL 218
           L+PVSW DYL++TVGGTLSN GISGQTFR+GPQI NV E+DVITGKGE  TCS   N +L
Sbjct: 167 LTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMNSDL 226

Query: 219 FYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQESLISRDQSNAPDYL 278
           F+++LGGLGQFGIITRARI L   P R KW+R LY +FS FT DQE +IS+  ++  D+L
Sbjct: 227 FFAVLGGLGQFGIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQERVISK--TDGVDFL 286

Query: 279 EGLLLLQLNAGD--KSSFYPLPDQPKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELET 338
           EG +++     D  +S++YP  D  +I+S+V ++ ++Y LE+VKYYD+ S  +V++E+E 
Sbjct: 287 EGSIMVDHGPPDNWRSTYYPPSDHLRIASMVKRHRVIYCLEVVKYYDETSQYTVNEEMEE 346

Query: 339 LLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDS 398
           L   L    G  + KD +Y +FL+RV T E+ L++ G W+VPHPW+NLFVPK++I+ FD 
Sbjct: 347 LSDSLNHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHPWLNLFVPKTQISKFDD 406

Query: 399 GVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           GVF+GII + N+TSG  L YPM +NK+
Sbjct: 407 GVFKGIILRNNITSGPVLVYPMNRNKW 424

[Close]
BLAST of CSPI01G28220 vs. ExPASy TrEMBL
Match: A0A1S3BQU4 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103492529 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 3.7e-217
Identity = 374/423 (88.42%), Postives = 396/423 (93.62%), Query Frame = 0 

Query: 1   MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH 60
           M++PN FLLIIFTCFLFLQNAPF  VA S+A PK + DKLK D  TISLASKDYGLMVQ 
Sbjct: 1   MESPNLFLLIIFTCFLFLQNAPFSAVALSLALPKTLLDKLKNDPLTISLASKDYGLMVQE 60

Query: 61  NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL 120
           NP  VFFP+TGHDV GLIRFMY  PVPLHIAARGQGHCVRGQALV +NG+VVNMTSLGG 
Sbjct: 61  NPSGVFFPLTGHDVVGLIRFMYTSPVPLHIAARGQGHCVRGQALV-ENGLVVNMTSLGGF 120

Query: 121 RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG 180
           RQKTSRIVV+TTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSW DYLHITVGGTLSNAG
Sbjct: 121 RQKTSRIVVSTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWTDYLHITVGGTLSNAG 180

Query: 181 ISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALA 240
           ISGQTFRFGPQINNV+ELDVITG+GE LTCSPTNNPELFYS+LGGLGQFGIITRARIALA
Sbjct: 181 ISGQTFRFGPQINNVHELDVITGRGELLTCSPTNNPELFYSVLGGLGQFGIITRARIALA 240

Query: 241 PTPTRVKWVRMLYTNFSSFTSDQESLISRDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQ 300
           PTPTRVKWVRMLYTNF+SFTSDQE+LISRD+SNAPDYLEGLL+LQ NA D SSFYPLPDQ
Sbjct: 241 PTPTRVKWVRMLYTNFASFTSDQETLISRDKSNAPDYLEGLLMLQFNANDDSSFYPLPDQ 300

Query: 301 PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD 360
           PKISSLVSQYGIVYVLE+VKYYDQ +SSSVDQE+E L+ GLKFEAGMKF+KD SYE+FLD
Sbjct: 301 PKISSLVSQYGIVYVLEIVKYYDQTTSSSVDQEVENLVAGLKFEAGMKFIKDVSYEQFLD 360

Query: 361 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 420
           RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK
Sbjct: 361 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 420

Query: 421 NKY 424
           NK+
Sbjct: 421 NKW 422

[Close]
BLAST of CSPI01G28220 vs. ExPASy TrEMBL
Match: A0A6J1HW04 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111466785 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 3.8e-185
Identity = 323/423 (76.36%), Postives = 367/423 (86.76%), Query Frame = 0 

Query: 4   PNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQHNPY 63
           PN FLLIIFT FL L NAPF   A +++ P+++++KL+ DLQTISLAS DYG ++Q NP 
Sbjct: 4   PNLFLLIIFTSFLSLHNAPFRASADNLSLPESLAEKLRDDLQTISLASTDYGRIIQENPI 63

Query: 64  AVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQK 123
            VFFPVTG+D+A  IR MY  P+ L IAARGQGH VRGQAL   NGVV+NMTSL   +Q 
Sbjct: 64  GVFFPVTGNDIAVFIRVMYAGPMRLDIAARGQGHSVRGQALAG-NGVVMNMTSLANSQQG 123

Query: 124 TSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISG 183
             RIVV TTSPLGPYADVG EQLWIDVLH T +KG SPVSW DYLHITVGGTLSNAGISG
Sbjct: 124 GGRIVVKTTSPLGPYADVGGEQLWIDVLHETMRKGFSPVSWTDYLHITVGGTLSNAGISG 183

Query: 184 QTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTP 243
           QTFRFGPQI+NVYELDV+TGKGESLTCSPT NPELFY++LGGLGQFG+ TRA+IALAPTP
Sbjct: 184 QTFRFGPQISNVYELDVVTGKGESLTCSPTTNPELFYAVLGGLGQFGVXTRAKIALAPTP 243

Query: 244 TRVKWVRMLYTNFSSFTSDQESLIS---RDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQ 303
           TRVKWVRMLYTNFS+FTSDQE LIS    ++SN  +++EGLLLLQL+  D SSF+PL DQ
Sbjct: 244 TRVKWVRMLYTNFSAFTSDQELLISNNGNEKSNGLNFVEGLLLLQLHTPDNSSFFPLADQ 303

Query: 304 PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD 363
           PKI+SLVSQYGIVYVLE+VKYYDQHS+SSVDQEL+ LLGGLKFE G+KFV+DASYE+FLD
Sbjct: 304 PKIASLVSQYGIVYVLEVVKYYDQHSASSVDQELDELLGGLKFEEGLKFVRDASYEQFLD 363

Query: 364 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 423
           RVHTDEV LR+LGLW+VPHPW+NLF+PKSRIADFDSGVFRGIIQKRN+TSGVFL YPM K
Sbjct: 364 RVHTDEVVLRSLGLWDVPHPWLNLFIPKSRIADFDSGVFRGIIQKRNITSGVFLIYPMLK 423

[Close]
BLAST of CSPI01G28220 vs. ExPASy TrEMBL
Match: A0A6J1EZ50 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111440877 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 7.9e-183
Identity = 322/423 (76.12%), Postives = 363/423 (85.82%), Query Frame = 0 

Query: 4   PNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQHNPY 63
           PN FLLIIFT FL L NAPF+  A +++ P +++ KL+ D QTISLAS DYG ++Q NP 
Sbjct: 4   PNLFLLIIFTSFLSLHNAPFWAAADNLSLPASLAGKLRDDPQTISLASTDYGRIIQENPI 63

Query: 64  AVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQK 123
            VFFPVTG+D+A  I  MY  P+ L IAARGQGH VRGQAL  +NGVV+NMTSL   RQ 
Sbjct: 64  GVFFPVTGNDIAFFIWVMYAGPIRLDIAARGQGHSVRGQALA-RNGVVMNMTSLANSRQG 123

Query: 124 TSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISG 183
             RIVV TTS LGPYADVG EQLWIDVLH T +KG SPVSW DYLHITVGGTLSNAGISG
Sbjct: 124 GGRIVVKTTSSLGPYADVGGEQLWIDVLHETMRKGFSPVSWTDYLHITVGGTLSNAGISG 183

Query: 184 QTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTP 243
           QTFRFGPQI+NVYELDV+TGKGESLTCSPT NPELFY++LGGLGQFG+ITRARIALAPTP
Sbjct: 184 QTFRFGPQISNVYELDVVTGKGESLTCSPTTNPELFYAVLGGLGQFGVITRARIALAPTP 243

Query: 244 TRVKWVRMLYTNFSSFTSDQESLIS---RDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQ 303
           TRVKWVRMLYTNFS+FTSDQE LIS    ++SN  +++EGLLLLQL+  D SSFYPL DQ
Sbjct: 244 TRVKWVRMLYTNFSAFTSDQELLISNKGNEKSNGLNFVEGLLLLQLHTPDNSSFYPLADQ 303

Query: 304 PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD 363
           PKI+SLVSQYGIVYVLE+VKYYDQHS+SSVDQEL  LLG LKFE G+KFV+DASYE+FLD
Sbjct: 304 PKIASLVSQYGIVYVLEVVKYYDQHSASSVDQELNELLGELKFEEGLKFVRDASYEQFLD 363

Query: 364 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 423
           RVHTDEVALR+LGLW+VPHPW+NLF+PKSRIADF+ GVFRGIIQKRN+TSGVFL YPM K
Sbjct: 364 RVHTDEVALRSLGLWDVPHPWLNLFIPKSRIADFELGVFRGIIQKRNITSGVFLVYPMLK 423

[Close]
BLAST of CSPI01G28220 vs. ExPASy TrEMBL
Match: A0A6J1D1E6 (Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111016468 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.2e-178
Identity = 313/424 (73.82%), Postives = 358/424 (84.43%), Query Frame = 0 

Query: 1   MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH 60
           M +PN F   +FT FL LQNAPF  VA S+  PKAI++KL+ DLQTI+LAS DYG +++ 
Sbjct: 1   MASPN-FFFFLFTYFLILQNAPFCAVARSLVLPKAIAEKLRTDLQTINLASTDYGRIIKQ 60

Query: 61  NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL 120
           NP+AVFFPVT +D+AGLIRFMY  P P ++AARGQGH VRGQALV  NGVVVNMTSL   
Sbjct: 61  NPFAVFFPVTANDIAGLIRFMYASPTPYYMAARGQGHSVRGQALV-PNGVVVNMTSLASF 120

Query: 121 RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG 180
           RQ   RIVV+T +    YADVG EQLWIDVLHAT  KGL+PVSW DYLHITVGGTLSNAG
Sbjct: 121 RQGAGRIVVSTAA---SYADVGGEQLWIDVLHATMHKGLTPVSWTDYLHITVGGTLSNAG 180

Query: 181 ISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALA 240
           ISGQTFRFGPQI+NVYELDV+TGKGE LTCSP  NPELFY++LGGLGQFG+ITRARI LA
Sbjct: 181 ISGQTFRFGPQISNVYELDVVTGKGEFLTCSPNTNPELFYAVLGGLGQFGVITRARIGLA 240

Query: 241 PTPTRVKWVRMLYTNFSSFTSDQESLIS---RDQSNAPDYLEGLLLLQLNAGDKSSFYPL 300
           P PT+VKWVRMLYT+FS+ T DQE LIS   +++SN  +Y+EGLL+L L+  D SSF+PL
Sbjct: 241 PAPTKVKWVRMLYTDFSAITRDQELLISNNGKEKSNGLNYVEGLLMLHLSTPDNSSFFPL 300

Query: 301 PDQPKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEE 360
            DQP+ISSLVSQYGIVYVLE+ KYYDQ S+SSVDQELE LLGGL+FE G KF+KD +YEE
Sbjct: 301 ADQPRISSLVSQYGIVYVLEVAKYYDQQSASSVDQELEGLLGGLRFEPGFKFIKDVTYEE 360

Query: 361 FLDRVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYP 420
           FLDRVH DE+ALRALGLW+VPHPW+NLF+PKSRI DFDSGVF+GIIQKRNLTSGV LFYP
Sbjct: 361 FLDRVHNDELALRALGLWDVPHPWLNLFIPKSRIVDFDSGVFKGIIQKRNLTSGVVLFYP 419

Query: 421 MFKN 422
           MFKN
Sbjct: 421 MFKN 419

[Close]
BLAST of CSPI01G28220 vs. ExPASy TrEMBL
Match: A0A6J1HCQ3 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111461627 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 5.5e-176
Identity = 310/426 (72.77%), Postives = 356/426 (83.57%), Query Frame = 0 

Query: 1   MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH 60
           M A N FLLIIF C L LQNAPF  VA  +  P+ IS KL+ D QTI LAS DYG +V+ 
Sbjct: 1   MAAHNNFLLIIFNCVLILQNAPFLAVA--LPLPEPISAKLRNDSQTIKLASTDYGRIVEE 60

Query: 61  NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL 120
            PY VFFPV G D+AGLI+FM   P P++IAARG+GH V+GQ+LV +NG+VVNMTSLGGL
Sbjct: 61  KPYGVFFPVNGSDIAGLIQFMNGSPTPMNIAARGRGHSVQGQSLV-ENGIVVNMTSLGGL 120

Query: 121 RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG 180
                RIVV+TT+ LGPYADVG EQLWIDVLHAT  KGLSP+SW DYL +TVGGTLSNAG
Sbjct: 121 ---DGRIVVSTTTALGPYADVGGEQLWIDVLHATMAKGLSPLSWTDYLRLTVGGTLSNAG 180

Query: 181 ISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALA 240
           I GQTFRFGPQI+NV ELDV+TGKGE LTCSPTNNPELFY  LGGLGQFG+ITRARI L 
Sbjct: 181 IGGQTFRFGPQISNVLELDVVTGKGELLTCSPTNNPELFYGALGGLGQFGVITRARIPLG 240

Query: 241 PTPTRVKWVRMLYTNFSSFTSDQESLIS---RDQSNAPDYLEGLLLLQLNAGDKSSFYPL 300
           P PTR KWVRMLYTNFS+FTSDQE LIS   +++SN  +YLEGLLLL+L A D SSFYPL
Sbjct: 241 PAPTRAKWVRMLYTNFSAFTSDQELLISNNGKEKSNGLNYLEGLLLLELKAPDNSSFYPL 300

Query: 301 PDQPKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEE 360
           PDQPKISSL+SQYGIVYVLE+VKYYDQ S+ SVDQ LE LL GL+FEAG+KF+KD SYEE
Sbjct: 301 PDQPKISSLISQYGIVYVLEVVKYYDQQSAGSVDQNLEKLLTGLRFEAGLKFIKDVSYEE 360

Query: 361 FLDRVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYP 420
           FLDRVHTDE+ALR+LGLW+VPHPW+NLF+PKS++ +F+ GV+R IIQKR L+SGV LFYP
Sbjct: 361 FLDRVHTDELALRSLGLWDVPHPWLNLFIPKSKLFEFEWGVYRSIIQKRKLSSGVLLFYP 420

Query: 421 MFKNKY 424
           +FKNK+
Sbjct: 421 IFKNKW 420

[Close]
BLAST of CSPI01G28220 vs. NCBI nr
Match: XP_004144113.1 (cytokinin dehydrogenase 2 [Cucumis sativus] >KAE8653432.1 hypothetical protein Csa_006855 [Cucumis sativus])

HSP 1 Score: 855.1 bits (2208), Expect = 2.5e-244
Identity = 421/423 (99.53%), Postives = 422/423 (99.76%), Query Frame = 0 

Query: 1   MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH 60
           MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH
Sbjct: 1   MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH 60

Query: 61  NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL 120
           NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL
Sbjct: 61  NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL 120

Query: 121 RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG 180
           RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG
Sbjct: 121 RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG 180

Query: 181 ISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALA 240
           ISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALA
Sbjct: 181 ISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALA 240

Query: 241 PTPTRVKWVRMLYTNFSSFTSDQESLISRDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQ 300
           PTPTRVKWVRMLYTNFSSFTSDQESLISRD SNAPDYLEGLLLLQLNAGDKSSFYPLPDQ
Sbjct: 241 PTPTRVKWVRMLYTNFSSFTSDQESLISRDPSNAPDYLEGLLLLQLNAGDKSSFYPLPDQ 300

Query: 301 PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD 360
           PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD
Sbjct: 301 PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD 360

Query: 361 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 420
           RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK
Sbjct: 361 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 420

Query: 421 NKY 424
           NK+
Sbjct: 421 NKW 423

[Close]
BLAST of CSPI01G28220 vs. NCBI nr
Match: XP_008451152.1 (PREDICTED: cytokinin dehydrogenase 4 [Cucumis melo])

HSP 1 Score: 763.8 bits (1971), Expect = 7.7e-217
Identity = 374/423 (88.42%), Postives = 396/423 (93.62%), Query Frame = 0 

Query: 1   MDAPNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQH 60
           M++PN FLLIIFTCFLFLQNAPF  VA S+A PK + DKLK D  TISLASKDYGLMVQ 
Sbjct: 1   MESPNLFLLIIFTCFLFLQNAPFSAVALSLALPKTLLDKLKNDPLTISLASKDYGLMVQE 60

Query: 61  NPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGL 120
           NP  VFFP+TGHDV GLIRFMY  PVPLHIAARGQGHCVRGQALV +NG+VVNMTSLGG 
Sbjct: 61  NPSGVFFPLTGHDVVGLIRFMYTSPVPLHIAARGQGHCVRGQALV-ENGLVVNMTSLGGF 120

Query: 121 RQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAG 180
           RQKTSRIVV+TTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSW DYLHITVGGTLSNAG
Sbjct: 121 RQKTSRIVVSTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWTDYLHITVGGTLSNAG 180

Query: 181 ISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALA 240
           ISGQTFRFGPQINNV+ELDVITG+GE LTCSPTNNPELFYS+LGGLGQFGIITRARIALA
Sbjct: 181 ISGQTFRFGPQINNVHELDVITGRGELLTCSPTNNPELFYSVLGGLGQFGIITRARIALA 240

Query: 241 PTPTRVKWVRMLYTNFSSFTSDQESLISRDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQ 300
           PTPTRVKWVRMLYTNF+SFTSDQE+LISRD+SNAPDYLEGLL+LQ NA D SSFYPLPDQ
Sbjct: 241 PTPTRVKWVRMLYTNFASFTSDQETLISRDKSNAPDYLEGLLMLQFNANDDSSFYPLPDQ 300

Query: 301 PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD 360
           PKISSLVSQYGIVYVLE+VKYYDQ +SSSVDQE+E L+ GLKFEAGMKF+KD SYE+FLD
Sbjct: 301 PKISSLVSQYGIVYVLEIVKYYDQTTSSSVDQEVENLVAGLKFEAGMKFIKDVSYEQFLD 360

Query: 361 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 420
           RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK
Sbjct: 361 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 420

Query: 421 NKY 424
           NK+
Sbjct: 421 NKW 422

[Close]
BLAST of CSPI01G28220 vs. NCBI nr
Match: XP_038879415.1 (cytokinin dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 688.0 bits (1774), Expect = 5.4e-194
Identity = 340/398 (85.43%), Postives = 362/398 (90.95%), Query Frame = 0 

Query: 26  VAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRFMYMLP 85
           VA S+A PKAI +KL+ D QTISLAS DYG +VQ NPY VFFPVTGHD+AGLIRFMY  P
Sbjct: 56  VALSLALPKAILEKLRNDPQTISLASTDYGRIVQENPYGVFFPVTGHDIAGLIRFMYASP 115

Query: 86  VPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQ 145
           VP  IAARGQGHCVRGQALV +NGVVVNMTSLG  RQKTSRIVV+TTS LGPYADVGAEQ
Sbjct: 116 VPWDIAARGQGHCVRGQALV-ENGVVVNMTSLGVFRQKTSRIVVSTTSQLGPYADVGAEQ 175

Query: 146 LWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKG 205
           LWIDVLHAT QKG SPVSW DYLHITVGGTLSNAGISGQTFRFGPQI+NV+ELDV+TGKG
Sbjct: 176 LWIDVLHATMQKGFSPVSWTDYLHITVGGTLSNAGISGQTFRFGPQISNVHELDVVTGKG 235

Query: 206 ESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQES 265
           E LTCSPTNNPELFYS+LGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQE 
Sbjct: 236 ELLTCSPTNNPELFYSVLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQEF 295

Query: 266 LISRDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQPKISSLVSQYGIVYVLELVKYYDQH 325
           L S+  SN PDY+EGLL+LQ N  D SSFYPL DQPKISSLVSQYGIVYVLE+VKYYDQ 
Sbjct: 296 LTSK--SNGPDYVEGLLMLQFNTPDNSSFYPLADQPKISSLVSQYGIVYVLEIVKYYDQQ 355

Query: 326 SSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLF 385
           SSSS+DQE+E LL GL FEAGMKF+KDASYEEFLDRVHTDEVAL ALGLW+VPHPW+NLF
Sbjct: 356 SSSSIDQEVEKLLRGLSFEAGMKFIKDASYEEFLDRVHTDEVALTALGLWDVPHPWMNLF 415

Query: 386 VPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           VPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK+K+
Sbjct: 416 VPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFKSKW 450

[Close]
BLAST of CSPI01G28220 vs. NCBI nr
Match: XP_023531793.1 (cytokinin dehydrogenase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.8 bits (1701), Expect = 1.6e-185
Identity = 325/423 (76.83%), Postives = 369/423 (87.23%), Query Frame = 0 

Query: 4   PNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQHNPY 63
           PN FLLIIFT FL LQNAPF+  A +++ P+++++KL+ D QTISLAS DYG ++Q NP 
Sbjct: 4   PNLFLLIIFTYFLSLQNAPFWAAADNLSLPESLAEKLRDDPQTISLASTDYGRIIQENPI 63

Query: 64  AVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQK 123
           AVFFPVTG+D+A  IR MY  P+ L IAARGQGH VRGQAL  +NGVV+NMTSL   RQ 
Sbjct: 64  AVFFPVTGNDIAFFIRVMYAGPIRLDIAARGQGHSVRGQALA-RNGVVMNMTSLANSRQG 123

Query: 124 TSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISG 183
             RIVV TTS LGPYADVG EQLWIDVLH T +KG SPVSW DYLH+TVGGTLSNAGISG
Sbjct: 124 GGRIVVKTTSSLGPYADVGGEQLWIDVLHETMRKGFSPVSWTDYLHMTVGGTLSNAGISG 183

Query: 184 QTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTP 243
           QTFRFGPQI+NVYELDV+TGKGESLTCSPT NPELFY++LGGLGQFG+ITRA+IALAPTP
Sbjct: 184 QTFRFGPQISNVYELDVVTGKGESLTCSPTTNPELFYAVLGGLGQFGVITRAKIALAPTP 243

Query: 244 TRVKWVRMLYTNFSSFTSDQESLIS---RDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQ 303
           TRVKWVRMLYTNFS+FTSDQE LIS    ++SN  +++EGLLLLQL+  D SSFYPL DQ
Sbjct: 244 TRVKWVRMLYTNFSAFTSDQELLISNNGNEKSNGLNFVEGLLLLQLHTPDNSSFYPLADQ 303

Query: 304 PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD 363
           PKI+SLVSQYGIVYVLE+VKYYDQHS+SSVDQEL  LLG LKFE G+KFV+DASYE+FLD
Sbjct: 304 PKIASLVSQYGIVYVLEVVKYYDQHSASSVDQELNELLGELKFEEGLKFVRDASYEQFLD 363

Query: 364 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 423
           RVHTDEVALR+LGLW+VPHPW+NLF+PKSRIADFDSGVFRGIIQKRN+TSGVFL YPM K
Sbjct: 364 RVHTDEVALRSLGLWDVPHPWLNLFIPKSRIADFDSGVFRGIIQKRNITSGVFLIYPMLK 423

[Close]
BLAST of CSPI01G28220 vs. NCBI nr
Match: XP_022967169.1 (cytokinin dehydrogenase 2-like [Cucurbita maxima])

HSP 1 Score: 657.5 bits (1695), Expect = 7.8e-185
Identity = 323/423 (76.36%), Postives = 367/423 (86.76%), Query Frame = 0 

Query: 4   PNPFLLIIFTCFLFLQNAPFFGVAFSVAFPKAISDKLKYDLQTISLASKDYGLMVQHNPY 63
           PN FLLIIFT FL L NAPF   A +++ P+++++KL+ DLQTISLAS DYG ++Q NP 
Sbjct: 4   PNLFLLIIFTSFLSLHNAPFRASADNLSLPESLAEKLRDDLQTISLASTDYGRIIQENPI 63

Query: 64  AVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQK 123
            VFFPVTG+D+A  IR MY  P+ L IAARGQGH VRGQAL   NGVV+NMTSL   +Q 
Sbjct: 64  GVFFPVTGNDIAVFIRVMYAGPMRLDIAARGQGHSVRGQALAG-NGVVMNMTSLANSQQG 123

Query: 124 TSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISG 183
             RIVV TTSPLGPYADVG EQLWIDVLH T +KG SPVSW DYLHITVGGTLSNAGISG
Sbjct: 124 GGRIVVKTTSPLGPYADVGGEQLWIDVLHETMRKGFSPVSWTDYLHITVGGTLSNAGISG 183

Query: 184 QTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTP 243
           QTFRFGPQI+NVYELDV+TGKGESLTCSPT NPELFY++LGGLGQFG+ TRA+IALAPTP
Sbjct: 184 QTFRFGPQISNVYELDVVTGKGESLTCSPTTNPELFYAVLGGLGQFGVXTRAKIALAPTP 243

Query: 244 TRVKWVRMLYTNFSSFTSDQESLIS---RDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQ 303
           TRVKWVRMLYTNFS+FTSDQE LIS    ++SN  +++EGLLLLQL+  D SSF+PL DQ
Sbjct: 244 TRVKWVRMLYTNFSAFTSDQELLISNNGNEKSNGLNFVEGLLLLQLHTPDNSSFFPLADQ 303

Query: 304 PKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLD 363
           PKI+SLVSQYGIVYVLE+VKYYDQHS+SSVDQEL+ LLGGLKFE G+KFV+DASYE+FLD
Sbjct: 304 PKIASLVSQYGIVYVLEVVKYYDQHSASSVDQELDELLGGLKFEEGLKFVRDASYEQFLD 363

Query: 364 RVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFK 423
           RVHTDEV LR+LGLW+VPHPW+NLF+PKSRIADFDSGVFRGIIQKRN+TSGVFL YPM K
Sbjct: 364 RVHTDEVVLRSLGLWDVPHPWLNLFIPKSRIADFDSGVFRGIIQKRNITSGVFLIYPMLK 423

[Close]
BLAST of CSPI01G28220 vs. TAIR 10
Match: AT4G29740.2 (cytokinin oxidase 4 )

HSP 1 Score: 398.7 bits (1023), Expect = 6.1e-111
Identity = 209/404 (51.73%), Postives = 271/404 (67.08%), Query Frame = 0 

Query: 30  VAFPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRF--------- 89
           V  P +++  +  D  +IS AS D+G +   NP AV  P +  +VA L+RF         
Sbjct: 32  VFLPISLNLTVLTDPFSISAASHDFGNITDENPGAVLCPSSTTEVARLLRFANGGFSYNK 91

Query: 90  -MYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYA 149
                     +AARGQGH +RGQA     GVVVNMT L  +  K + +V++     G YA
Sbjct: 92  GSTSPASTFKVAARGQGHSLRGQASA-PGGVVVNMTCL-AMAAKPAAVVISAD---GTYA 151

Query: 150 DVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELD 209
           DV A  +W+DVL A   +G+SPV+W DYL+++VGGTLSNAGI GQTFR GPQI+NV+ELD
Sbjct: 152 DVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVGGTLSNAGIGGQTFRHGPQISNVHELD 211

Query: 210 VITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSF 269
           VITGKGE +TCSP  NPELFY +LGGLGQFGIITRARIAL   PTRVKW R+LY++FS+F
Sbjct: 212 VITGKGEMMTCSPKLNPELFYGVLGGLGQFGIITRARIALDHAPTRVKWSRILYSDFSAF 271

Query: 270 TSDQESLISRDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQPKISSLVSQYGIVYVLELV 329
             DQE LIS       D+LEG L++  N    +SF+PL DQ +++SLV+ + I+YVLE+ 
Sbjct: 272 KRDQERLISMTNDLGVDFLEGQLMMS-NGFVDTSFFPLSDQTRVASLVNDHRIIYVLEVA 331

Query: 330 KYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPH 389
           KYYD+ +   +DQ ++TL   L F  G  FV+D  Y +FL+RV  +E  LR+LGLWEVPH
Sbjct: 332 KYYDRTTLPIIDQVIDTLSRTLGFAPGFMFVQDVPYFDFLNRVRNEEDKLRSLGLWEVPH 391

Query: 390 PWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           PW+N+FVP SRI DF  GV  G++  +  TSGV LFYP  +NK+
Sbjct: 392 PWLNIFVPGSRIQDFHDGVINGLLLNQTSTSGVTLFYPTNRNKW 429

[Close]
BLAST of CSPI01G28220 vs. TAIR 10
Match: AT4G29740.1 (cytokinin oxidase 4 )

HSP 1 Score: 398.3 bits (1022), Expect = 7.9e-111
Identity = 209/403 (51.86%), Postives = 270/403 (67.00%), Query Frame = 0 

Query: 30  VAFPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRF--------- 89
           V  P +++  +  D  +IS AS D+G +   NP AV  P +  +VA L+RF         
Sbjct: 32  VFLPISLNLTVLTDPFSISAASHDFGNITDENPGAVLCPSSTTEVARLLRFANGGFSYNK 91

Query: 90  -MYMLPVPLHIAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYA 149
                     +AARGQGH +RGQA     GVVVNMT L  +  K + +V++     G YA
Sbjct: 92  GSTSPASTFKVAARGQGHSLRGQASA-PGGVVVNMTCL-AMAAKPAAVVISAD---GTYA 151

Query: 150 DVGAEQLWIDVLHATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELD 209
           DV A  +W+DVL A   +G+SPV+W DYL+++VGGTLSNAGI GQTFR GPQI+NV+ELD
Sbjct: 152 DVAAGTMWVDVLKAAVDRGVSPVTWTDYLYLSVGGTLSNAGIGGQTFRHGPQISNVHELD 211

Query: 210 VITGKGESLTCSPTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSF 269
           VITGKGE +TCSP  NPELFY +LGGLGQFGIITRARIAL   PTRVKW R+LY++FS+F
Sbjct: 212 VITGKGEMMTCSPKLNPELFYGVLGGLGQFGIITRARIALDHAPTRVKWSRILYSDFSAF 271

Query: 270 TSDQESLISRDQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQPKISSLVSQYGIVYVLELV 329
             DQE LIS       D+LEG L++  N    +SF+PL DQ +++SLV+ + I+YVLE+ 
Sbjct: 272 KRDQERLISMTNDLGVDFLEGQLMMS-NGFVDTSFFPLSDQTRVASLVNDHRIIYVLEVA 331

Query: 330 KYYDQHSSSSVDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPH 389
           KYYD+ +   +DQ ++TL   L F  G  FV+D  Y +FL+RV  +E  LR+LGLWEVPH
Sbjct: 332 KYYDRTTLPIIDQVIDTLSRTLGFAPGFMFVQDVPYFDFLNRVRNEEDKLRSLGLWEVPH 391

Query: 390 PWINLFVPKSRIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNK 423
           PW+N+FVP SRI DF  GV  G++  +  TSGV LFYP  +NK
Sbjct: 392 PWLNIFVPGSRIQDFHDGVINGLLLNQTSTSGVTLFYPTNRNK 428

[Close]
BLAST of CSPI01G28220 vs. TAIR 10
Match: AT2G19500.1 (cytokinin oxidase 2 )

HSP 1 Score: 382.5 bits (981), Expect = 4.5e-106
Identity = 198/394 (50.25%), Postives = 257/394 (65.23%), Query Frame = 0 

Query: 30  VAFPKAISDKLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRFMYMLPVPLH 89
           +  PK+++  L  D   IS AS D+G +    P  V  P +  D++ L+++         
Sbjct: 25  IDLPKSLNLTLSTDPSIISAASHDFGNITTVTPGGVICPSSTADISRLLQYAANGKSTFQ 84

Query: 90  IAARGQGHCVRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQLWID 149
           +AARGQGH + GQA V   GV+VNMT +       + +VV+       YADV A  LW+D
Sbjct: 85  VAARGQGHSLNGQASV-SGGVIVNMTCI-------TDVVVSKDK---KYADVAAGTLWVD 144

Query: 150 VLHATTQKGLSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKGESLT 209
           VL  T +KG+SPVSW DYLHITVGGTLSN GI GQ FR GP ++NV ELDVITGKGE LT
Sbjct: 145 VLKKTAEKGVSPVSWTDYLHITVGGTLSNGGIGGQVFRNGPLVSNVLELDVITGKGEMLT 204

Query: 210 CSPTNNPELFYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQESLISR 269
           CS   NPELFY +LGGLGQFGIITRARI L   P R KW RMLY++F++FT DQE LIS 
Sbjct: 205 CSRQLNPELFYGVLGGLGQFGIITRARIVLDHAPKRAKWFRMLYSDFTTFTKDQERLISM 264

Query: 270 DQSNAPDYLEGLLLLQLNAGDKSSFYPLPDQPKISSLVSQYGIVYVLELVKYYDQHSSSS 329
                 DYLEG + L     D +SF+P  DQ K++ LV Q+GI+YVLE+ KYYD  +   
Sbjct: 265 ANDIGVDYLEGQIFLSNGVVD-TSFFPPSDQSKVADLVKQHGIIYVLEVAKYYDDPNLPI 324

Query: 330 VDQELETLLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLFVPKS 389
           + + ++TL   L +  G   + D +Y +FL+RVH +E  LR+LGLWE+PHPW+NL+VPKS
Sbjct: 325 ISKVIDTLTKTLSYLPGFISMHDVAYFDFLNRVHVEENKLRSLGLWELPHPWLNLYVPKS 384

Query: 390 RIADFDSGVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           RI DF +GV + I+ K+   SG+ L YP  +NK+
Sbjct: 385 RILDFHNGVVKDILLKQKSASGLALLYPTNRNKW 406

[Close]
BLAST of CSPI01G28220 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 370.2 bits (949), Expect = 2.3e-102
Identity = 188/387 (48.58%), Postives = 261/387 (67.44%), Query Frame = 0 

Query: 39  KLKYDLQTISLASKDYGLMVQHNPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHC 98
           KL     ++  A+ D+G + +  P AV  P +  D+  LI+  +   +   +AARG GH 
Sbjct: 47  KLTSSSSSVESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFDSQLSFPLAARGHGHS 106

Query: 99  VRGQALVDQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKG 158
            RGQA   ++GVVVNM S+   R +  ++  T       Y DV A  LWI+VL+ T + G
Sbjct: 107 HRGQASA-KDGVVVNMRSMVN-RDRGIKVSRTCL-----YVDVDAAWLWIEVLNKTLELG 166

Query: 159 LSPVSWVDYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPEL 218
           L+PVSW DYL++TVGGTLSN GISGQTFR+GPQI NV E+DVITGKGE  TCS   N +L
Sbjct: 167 LTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMNSDL 226

Query: 219 FYSLLGGLGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQESLISRDQSNAPDYL 278
           F+++LGGLGQFGIITRARI L   P R KW+R LY +FS FT DQE +IS+  ++  D+L
Sbjct: 227 FFAVLGGLGQFGIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQERVISK--TDGVDFL 286

Query: 279 EGLLLLQLNAGD--KSSFYPLPDQPKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELET 338
           EG +++     D  +S++YP  D  +I+S+V ++ ++Y LE+VKYYD+ S  +V++E+E 
Sbjct: 287 EGSIMVDHGPPDNWRSTYYPPSDHLRIASMVKRHRVIYCLEVVKYYDETSQYTVNEEMEE 346

Query: 339 LLGGLKFEAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDS 398
           L   L    G  + KD +Y +FL+RV T E+ L++ G W+VPHPW+NLFVPK++I+ FD 
Sbjct: 347 LSDSLNHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHPWLNLFVPKTQISKFDD 406

Query: 399 GVFRGIIQKRNLTSGVFLFYPMFKNKY 424
           GVF+GII + N+TSG  L YPM +NK+
Sbjct: 407 GVFKGIILRNNITSGPVLVYPMNRNKW 424

[Close]
BLAST of CSPI01G28220 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 358.2 bits (918), Expect = 9.1e-99
Identity = 189/380 (49.74%), Postives = 241/380 (63.42%), Query Frame = 0 

Query: 47  ISLASKDYGLMVQ-HNPYAVFFPVTGHDVAGLIRFMYMLPVPLHIAARGQGHCVRGQALV 106
           ++  S D+G++     P AV  P +  DVA L+R  Y       ++ARG GH + GQA  
Sbjct: 51  LASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAYGSATAFPVSARGHGHSINGQAAA 110

Query: 107 DQNGVVVNMTSLGGLRQKTSRIVVTTTSPLGPYADVGAEQLWIDVLHATTQKGLSPVSWV 166
            +NGVVV M    G+      +V     P   Y DV   +LW+DVL  T + GL+P SW 
Sbjct: 111 GRNGVVVEMNH--GVTGTPKPLV----RPDEMYVDVWGGELWVDVLKKTLEHGLAPKSWT 170

Query: 167 DYLHITVGGTLSNAGISGQTFRFGPQINNVYELDVITGKGESLTCSPTNNPELFYSLLGG 226
           DYL++TVGGTLSNAGISGQ F  GPQI+NV ELDV+TGKGE + CS   N  LF+ +LGG
Sbjct: 171 DYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGVLGG 230

Query: 227 LGQFGIITRARIALAPTPTRVKWVRMLYTNFSSFTSDQESLISRDQSNAPDYLEGLLLLQ 286
           LGQFGIITRARI+L P P RV+W+R+LY++F  FT DQE LIS       DY+EG +++ 
Sbjct: 231 LGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKFDYVEGFVIVD 290

Query: 287 --LNAGDKSSFYPLPDQPKISSLVSQYGIVYVLELVKYYDQHSSSSVDQELETLLGGLKF 346
             L    +SSF+   +  KISS+ S   ++Y LE+ K Y    S  VDQE+E L+  L F
Sbjct: 291 EGLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSDSEIVDQEVEILMKKLNF 350

Query: 347 EAGMKFVKDASYEEFLDRVHTDEVALRALGLWEVPHPWINLFVPKSRIADFDSGVFRGII 406
                F  D  Y +FLDRVH  E+ LR+  LWEVPHPW+NLFVPKSRI+DFD GVF+GI+
Sbjct: 351 IPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKGVFKGIL 410

Query: 407 QKRNLTSGVFLFYPMFKNKY 424
              N TSG  L YPM K+K+
Sbjct: 411 --GNKTSGPILIYPMNKDKW 422

The following BLAST results are available for this feature:
BLAST of CSPI01G28220 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FUJ28.5e-11051.73Cytokinin dehydrogenase 4 OS=Arabidopsis thaliana OX=3702 GN=CKX4 PE=1 SV=2[more]
Q6YW504.9e-10549.75Cytokinin dehydrogenase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX7 PE=3 ... [more]
Q9FUJ36.3e-10550.25Cytokinin dehydrogenase 2 OS=Arabidopsis thaliana OX=3702 GN=CKX2 PE=1 SV=2[more]
Q6YW512.7e-10349.50Cytokinin dehydrogenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX6 PE=3 ... [more]
Q9LTS33.3e-10148.58Cytokinin dehydrogenase 3 OS=Arabidopsis thaliana OX=3702 GN=CKX3 PE=1 SV=1[more]
BLAST of CSPI01G28220 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BQU43.7e-21788.42Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103492529 PE=3 SV=1[more]
A0A6J1HW043.8e-18576.36Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111466785 PE=3 SV=1[more]
A0A6J1EZ507.9e-18376.12Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111440877 PE=3 SV=1[more]
A0A6J1D1E61.2e-17873.82Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111016468 PE=3 SV=1[more]
A0A6J1HCQ35.5e-17672.77Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111461627 PE=3 SV=1[more]
BLAST of CSPI01G28220 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004144113.12.5e-24499.53cytokinin dehydrogenase 2 [Cucumis sativus] >KAE8653432.1 hypothetical protein C... [more]
XP_008451152.17.7e-21788.42PREDICTED: cytokinin dehydrogenase 4 [Cucumis melo][more]
XP_038879415.15.4e-19485.43cytokinin dehydrogenase 2-like [Benincasa hispida][more]
XP_023531793.11.6e-18576.83cytokinin dehydrogenase 4-like [Cucurbita pepo subsp. pepo][more]
XP_022967169.17.8e-18576.36cytokinin dehydrogenase 2-like [Cucurbita maxima][more]
BLAST of CSPI01G28220 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G29740.26.1e-11151.73cytokinin oxidase 4 [more]
AT4G29740.17.9e-11151.86cytokinin oxidase 4 [more]
AT2G19500.14.5e-10650.25cytokinin oxidase 2 [more]
AT5G56970.12.3e-10248.58cytokinin oxidase 3 [more]
AT1G75450.19.1e-9949.74cytokinin oxidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 114..244
e-value: 1.6E-144
score: 484.1
IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domainPFAMPF09265Cytokin-bindcoord: 243..423
e-value: 3.3E-61
score: 207.2
IPR016170Cytokinin dehydrogenase, C-terminal domain superfamilyGENE3D3.40.462.10coord: 245..423
e-value: 1.6E-144
score: 484.1
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 63..211
e-value: 7.6E-12
score: 45.2
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 44..113
e-value: 1.6E-144
score: 484.1
NoneNo IPR availablePANTHERPTHR13878:SF115CYTOKININ DEHYDROGENASE 1, FAD AND CYTOKININ-BINDING PROTEINcoord: 34..423
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 34..423
IPR006093Oxygen oxidoreductase covalent FAD-binding sitePROSITEPS00862OX2_COVAL_FADcoord: 62..97
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 58..242
score: 13.715775
IPR016164FAD-linked oxidase-like, C-terminalSUPERFAMILY55103FAD-linked oxidases, C-terminal domaincoord: 243..422
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 38..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G28220.1CSPI01G28220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009690 cytokinin metabolic process
molecular_function GO:0019139 cytokinin dehydrogenase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity