CSPI01G28030 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G28030
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytidine deaminase
LocationChr1: 22766632 .. 22767846 (+)
RNA-Seq ExpressionCSPI01G28030
SyntenyCSPI01G28030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCTCACAAAAAATAATTAATTTTTAGTTAATAATTGCTAATTAATCAATCAATTTCTTCTTCTCAACACTCTCTATTTATATTCCCTCTTCTTTTCGCTTCTCTTCCCCTTTCTCCTTTCTCCGCCACCGCCATTCTCACCGGGATATCAACTGAATTTCTCCCATCGCCGGCAATGGATGGAACTAGATTCGTAATCGAAGCACCAGAAGCTGAATCCTTGGCCAAACAATCAGCCCTCACAATTCCTCTCCTTCTTCCAACTCTCGTCAAATCGGCGCAAAGCTTAGCCCGGCCGCCGATCTCCAAGTACCACGTCGGCGCCGTCGGTCTCGGATCATCCGGTAGAGTGTTCTTCGGGGTCAATCTCGAGTTCCCCGGTCTTCCTCTTCATCAATCTGTTCACGCCGAACAATTCCTCGTCACAAATCTCGCCCTAAACGCCGAATCTCACCTCAATTACCTCGCCGTCTCCGCTGCCCCTTGCGGCCATTGCCGTCAATTCCTCCAAGAAGTTCGCTCTTCCGCAGATATCAAAATCCTCGTCTCCGATTCTGGTTCCGATTCTGGTTCCGATTCCAAACCCGACGTTTACGTTCCTCTCCCCCAATTTCTCCCCCACCGATTCGGCCCCTATGATCTTCTCGCTAAGGACGTTCCATTACTCCTCGAGCCACGATTTAACGGCTTTTCTCTTCCGAACGAATCCGCCGAAAATAATAAGCTCTGTAATGGCAATCACGGCGAGAATTTGGAGAAACTCAAACGCGCCGCCTTAGATGCCGCGAATATGTCCCACGCGCCGTACAGTAAATGTCCGTCGGGAGTTGCGCTGATGGACGACAATGGACGAATCTACAATGGACCCTACATGGAATCAGCTGCTTATAATCCGAGTATGGGGCCAGTACAGGCGGCGATAGTGGCGTACATCGCCGGGGGCGGTGCTGGGTATGAGAGAATCGTCGCGGCCGTGTTGGTGGAGAAGGACGGCGTGGAAGTCAAGCAAGAACGGGCGGCGAGGCTTTTACTGGAGACGATCTCGCCGGAGTGTGAATTTACGGTTGTTCATTGTATCGCGGCCGTGTGATAGTGGATCATTTTTTTTTTTTAATTTGTAAATTTTGAATCACAATTTCTTTGATGTTTTTAGACTGTTTGATGCATATCATGTAATATTGTAACGAAATAAATTATATAAAAATCCCTCTTTCT

mRNA sequence

GCCTCACAAAAAATAATTAATTTTTAGTTAATAATTGCTAATTAATCAATCAATTTCTTCTTCTCAACACTCTCTATTTATATTCCCTCTTCTTTTCGCTTCTCTTCCCCTTTCTCCTTTCTCCGCCACCGCCATTCTCACCGGGATATCAACTGAATTTCTCCCATCGCCGGCAATGGATGGAACTAGATTCGTAATCGAAGCACCAGAAGCTGAATCCTTGGCCAAACAATCAGCCCTCACAATTCCTCTCCTTCTTCCAACTCTCGTCAAATCGGCGCAAAGCTTAGCCCGGCCGCCGATCTCCAAGTACCACGTCGGCGCCGTCGGTCTCGGATCATCCGGTAGAGTGTTCTTCGGGGTCAATCTCGAGTTCCCCGGTCTTCCTCTTCATCAATCTGTTCACGCCGAACAATTCCTCGTCACAAATCTCGCCCTAAACGCCGAATCTCACCTCAATTACCTCGCCGTCTCCGCTGCCCCTTGCGGCCATTGCCGTCAATTCCTCCAAGAAGTTCGCTCTTCCGCAGATATCAAAATCCTCGTCTCCGATTCTGGTTCCGATTCTGGTTCCGATTCCAAACCCGACGTTTACGTTCCTCTCCCCCAATTTCTCCCCCACCGATTCGGCCCCTATGATCTTCTCGCTAAGGACGTTCCATTACTCCTCGAGCCACGATTTAACGGCTTTTCTCTTCCGAACGAATCCGCCGAAAATAATAAGCTCTGTAATGGCAATCACGGCGAGAATTTGGAGAAACTCAAACGCGCCGCCTTAGATGCCGCGAATATGTCCCACGCGCCGTACAGTAAATGTCCGTCGGGAGTTGCGCTGATGGACGACAATGGACGAATCTACAATGGACCCTACATGGAATCAGCTGCTTATAATCCGAGTATGGGGCCAGTACAGGCGGCGATAGTGGCGTACATCGCCGGGGGCGGTGCTGGGTATGAGAGAATCGTCGCGGCCGTGTTGGTGGAGAAGGACGGCGTGGAAGTCAAGCAAGAACGGGCGGCGAGGCTTTTACTGGAGACGATCTCGCCGGAGTGTGAATTTACGGTTGTTCATTGTATCGCGGCCGTGTGATAGTGGATCATTTTTTTTTTTTAATTTGTAAATTTTGAATCACAATTTCTTTGATGTTTTTAGACTGTTTGATGCATATCATGTAATATTGTAACGAAATAAATTATATAAAAATCCCTCTTTCT

Coding sequence (CDS)

ATGGATGGAACTAGATTCGTAATCGAAGCACCAGAAGCTGAATCCTTGGCCAAACAATCAGCCCTCACAATTCCTCTCCTTCTTCCAACTCTCGTCAAATCGGCGCAAAGCTTAGCCCGGCCGCCGATCTCCAAGTACCACGTCGGCGCCGTCGGTCTCGGATCATCCGGTAGAGTGTTCTTCGGGGTCAATCTCGAGTTCCCCGGTCTTCCTCTTCATCAATCTGTTCACGCCGAACAATTCCTCGTCACAAATCTCGCCCTAAACGCCGAATCTCACCTCAATTACCTCGCCGTCTCCGCTGCCCCTTGCGGCCATTGCCGTCAATTCCTCCAAGAAGTTCGCTCTTCCGCAGATATCAAAATCCTCGTCTCCGATTCTGGTTCCGATTCTGGTTCCGATTCCAAACCCGACGTTTACGTTCCTCTCCCCCAATTTCTCCCCCACCGATTCGGCCCCTATGATCTTCTCGCTAAGGACGTTCCATTACTCCTCGAGCCACGATTTAACGGCTTTTCTCTTCCGAACGAATCCGCCGAAAATAATAAGCTCTGTAATGGCAATCACGGCGAGAATTTGGAGAAACTCAAACGCGCCGCCTTAGATGCCGCGAATATGTCCCACGCGCCGTACAGTAAATGTCCGTCGGGAGTTGCGCTGATGGACGACAATGGACGAATCTACAATGGACCCTACATGGAATCAGCTGCTTATAATCCGAGTATGGGGCCAGTACAGGCGGCGATAGTGGCGTACATCGCCGGGGGCGGTGCTGGGTATGAGAGAATCGTCGCGGCCGTGTTGGTGGAGAAGGACGGCGTGGAAGTCAAGCAAGAACGGGCGGCGAGGCTTTTACTGGAGACGATCTCGCCGGAGTGTGAATTTACGGTTGTTCATTGTATCGCGGCCGTGTGA

Protein sequence

MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFFGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADIKILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNPSMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHCIAAV*
Homology
BLAST of CSPI01G28030 vs. ExPASy Swiss-Prot
Match: O65896 (Cytidine deaminase 1 OS=Arabidopsis thaliana OX=3702 GN=CDA1 PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.8e-99
Identity = 191/306 (62.42%), Postives = 231/306 (75.49%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MD   FVI++ EAES AKQ  +++  LLP+LVK AQS AR PISK++V  VGLGSSGR+F
Sbjct: 1   MDKPSFVIQSKEAESAAKQLGVSVIQLLPSLVKPAQSYARTPISKFNVAVVGLGSSGRIF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
            GVN+EFP LPLH S+HAEQFLVTNL LN E HLN+ AVSAAPCGHCRQFLQE+R + +I
Sbjct: 61  LGVNVEFPNLPLHHSIHAEQFLVTNLTLNGERHLNFFAVSAAPCGHCRQFLQEIRDAPEI 120

Query: 121 KILVSDSGSDSGSDSKPDV--YVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNES 180
           KIL++D  + + SDS  D   ++ L  FLPHRFGP DLL KD PLLLE   N   +    
Sbjct: 121 KILITDPNNSADSDSAADSDGFLRLGSFLPHRFGPDDLLGKDHPLLLESHDNHLKI---- 180

Query: 181 AENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAY 240
           ++ + +CNGN   + + LK+ AL AAN S+APYS CPSGV+L+D +G++Y G YMESAAY
Sbjct: 181 SDLDSICNGNTDSSAD-LKQTALAAANRSYAPYSLCPSGVSLVDCDGKVYRGWYMESAAY 240

Query: 241 NPSMGPVQAAIVAYIA-GGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTV 300
           NPSMGPVQAA+V Y+A GGG GYERIV AVLVEK+   V+QE  ARLLLETISP+CEF V
Sbjct: 241 NPSMGPVQAALVDYVANGGGGGYERIVGAVLVEKEDAVVRQEHTARLLLETISPKCEFKV 300

Query: 301 VHCIAA 304
            HC  A
Sbjct: 301 FHCYEA 301

BLAST of CSPI01G28030 vs. ExPASy Swiss-Prot
Match: Q9SU87 (Cytidine deaminase 7 OS=Arabidopsis thaliana OX=3702 GN=CDA7 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.2e-70
Identity = 158/309 (51.13%), Postives = 197/309 (63.75%), Query Frame = 0

Query: 2   DGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFF 61
           D  +FV  A EA S      +T P+ LP L++ A SLAR PISKY VGAVG  SSGRV+ 
Sbjct: 5   DKYKFVFTAKEAAS----EGVTEPIRLPKLIRKAMSLARGPISKYKVGAVGRASSGRVYL 64

Query: 62  GVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVS--------AAPCGHCRQFLQE 121
           GVN+EFPGLPLH S+H EQFLVTNLALN+E  L  LAV+         APCG+CRQFL E
Sbjct: 65  GVNVEFPGLPLHHSIHPEQFLVTNLALNSEKGLRQLAVAISSDCIEFGAPCGNCRQFLME 124

Query: 122 VRSSADIKILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFS 181
             +  DIKIL+       GS S       L   LP+RF P D+L K  PLLLE R N  +
Sbjct: 125 TSNELDIKILLKSKHEAEGSFSS------LKLLLPYRFTPDDVLPKGSPLLLEKRDNCLT 184

Query: 182 LPNESAENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYM 241
           L   S    ++C+     +   LK  AL AAN S +PY++ PSGVAL DD G+ Y G Y+
Sbjct: 185 L---SGSTEEICS----SDCSHLKCKALAAANNSFSPYTESPSGVALQDDEGKWYRGWYI 244

Query: 242 ESAAYNPSMGPVQAAIVAYIA-GGGAGYERIVAAVLVEKDGVEVKQERAARLLLETI-SP 301
           ES AY+PS+GPVQAA+V ++A   G G+ +IV AVLVEK+   V QER A+++L+TI +P
Sbjct: 245 ESVAYSPSLGPVQAALVDFVARSRGKGFNKIVEAVLVEKNNARVSQERTAKMILDTIAAP 296

BLAST of CSPI01G28030 vs. ExPASy Swiss-Prot
Match: Q9SU86 (Cytidine deaminase 6 OS=Arabidopsis thaliana OX=3702 GN=CDA6 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.7e-65
Identity = 143/293 (48.81%), Postives = 187/293 (63.82%), Query Frame = 0

Query: 15  SLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFFGVNLEFPGLPLHQ 74
           S A +  +  P  LP L+  A SLAR P+S + VGAVGL SSG VF GVN+EFP LPLH 
Sbjct: 8   SEAAEEGVRGPSDLPKLIDKAMSLARAPVSTFKVGAVGLTSSGEVFLGVNVEFPNLPLHH 67

Query: 75  SVHAEQFLVTNLALNAESHLNYLAVS------AAPCGHCRQFLQEVRSSADIKILVSDSG 134
           ++HAEQFLVTNLALN+   L ++AVS       APCGHCRQF QE+R++ +I+IL+    
Sbjct: 68  TIHAEQFLVTNLALNSMKKLTHIAVSVTGTIFGAPCGHCRQFYQEMRNAPEIEILIKRPK 127

Query: 135 SDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAENNKLCNGN 194
                    D ++ L   +P RFGP  +L +D  LLLE R N   L    ++  ++C  +
Sbjct: 128 DGI------DEFMSLKSLMPERFGPDSILPEDASLLLEQRDNSLVL----SDPEEIC--S 187

Query: 195 HGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNPSMGPVQAA 254
             E+    K  AL AAN S+APYSKCPSGVAL+   G +Y G Y+ES AYNPS+GPV+AA
Sbjct: 188 DPEDCSHTKCRALAAANKSYAPYSKCPSGVALI-CGGEVYKGWYIESVAYNPSLGPVEAA 247

Query: 255 IVAYIA-GGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 301
           +V ++A GGG  +  I   VLVE   V+V QE  AR  L+ I+P+C+F V+HC
Sbjct: 248 LVDFVARGGGKEFNEITEVVLVEMKDVKVSQEATARTFLDKIAPKCDFKVLHC 287

BLAST of CSPI01G28030 vs. ExPASy Swiss-Prot
Match: O65571 (Cytidine deaminase 2 OS=Arabidopsis thaliana OX=3702 GN=CDA2 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.6e-63
Identity = 146/308 (47.40%), Postives = 193/308 (62.66%), Query Frame = 0

Query: 2   DGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFF 61
           D  +FV  A E    A    +T P+ LP L++ A  LAR PISKY VGAVG  SSGRV+ 
Sbjct: 26  DRFKFVFTANE----AALEGVTDPIRLPNLIRKAMCLARAPISKYKVGAVGRASSGRVYL 85

Query: 62  GVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVS--------AAPCGHCRQFLQE 121
           GVN++FPGLPLH S+HAEQFLVTNLALN E  L  LAV+          PCG+C QFL E
Sbjct: 86  GVNVDFPGLPLHHSIHAEQFLVTNLALNYEKDLCKLAVAISTDGLEFGTPCGNCLQFLME 145

Query: 122 VRSSADIKILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFS 181
           + ++ D+KIL S    ++GS S   + +P            ++L K  P LLE R+N  +
Sbjct: 146 MSNALDMKIL-SKPKHEAGSFSSLRLLLP------------NVLPKGSPFLLEKRYNCLT 205

Query: 182 LPNESAENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYM 241
           L   + E   +C+     +   LKR AL AAN S +PY++ PSGVAL+D++G  Y G Y+
Sbjct: 206 LSGSAGE---ICS----LDCSHLKRRALAAANNSFSPYTESPSGVALLDNDGNWYRGWYI 265

Query: 242 ESAAYNPSMGPVQAAIVAYIA-GGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPE 301
           ES A NPS+GPVQAA+V ++A   G  + +IV AVLVEK+   V QER A+++L+TI+P 
Sbjct: 266 ESVASNPSLGPVQAALVDFVARSRGKMFNKIVQAVLVEKNNASVSQERTAKIILDTIAPN 309

BLAST of CSPI01G28030 vs. ExPASy Swiss-Prot
Match: Q9XEX4 (Cytidine deaminase 8 OS=Arabidopsis thaliana OX=3702 GN=CDA8 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.0e-54
Identity = 138/304 (45.39%), Postives = 181/304 (59.54%), Query Frame = 0

Query: 5   RFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFFGVN 64
           RF++   E ES     A T P  L  L+  A    R  IS   V AVG GSSGR FFGVN
Sbjct: 6   RFMLNHIETES---YGAFT-PQNLSPLINRAIPHTRAQISGSPVVAVGRGSSGRTFFGVN 65

Query: 65  LEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSA------APCGHCRQFLQEVRSSA 124
           +E PGLPL  S+HAEQFL+ NLAL+ E  L  +A+S        PCGHC Q L ++R  +
Sbjct: 66  VELPGLPLDHSIHAEQFLLANLALHFEQKLECIAISTNGYYFQEPCGHCCQLLHKIRDMS 125

Query: 125 DIKILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNES 184
           D KIL+++     G+      Y+ L  FLP       +   +VP LLE  FN   L N S
Sbjct: 126 DTKILLTNPTGQKGT------YMNLSTFLPQGL----ISQANVPRLLERNFNCIELINHS 185

Query: 185 AENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAY 244
              + +C  ++ E+   L   AL AA +S+AP SKCPSGVAL+D  G++Y+G YMES A+
Sbjct: 186 LYMD-IC--SYSEHCNHLNCRALKAATISYAPDSKCPSGVALIDHRGKVYSGGYMESVAH 245

Query: 245 NPSMGPVQAAIVAYIAGG-GAGYERIVAAVLVEKDGVEVKQERAARLLLETIS-PECEFT 301
           N S+GPVQAA+V ++A G G  ++ IV AVLVEK    + QE  AR++LE I+ P+C F 
Sbjct: 246 NTSLGPVQAALVDFVANGDGQEFKNIVEAVLVEKKCGVLSQEATARMILEKIADPDCIFR 292

BLAST of CSPI01G28030 vs. ExPASy TrEMBL
Match: A0A0A0LYU5 (Cytidine deaminase OS=Cucumis sativus OX=3659 GN=Csa_1G587400 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.3e-167
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI
Sbjct: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNG SLPNE+AE
Sbjct: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGLSLPNETAE 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP
Sbjct: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC
Sbjct: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300

Query: 301 IAAV 305
           IAAV
Sbjct: 301 IAAV 304

BLAST of CSPI01G28030 vs. ExPASy TrEMBL
Match: A0A5D3CE07 (Cytidine deaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002450 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.3e-158
Identity = 291/304 (95.72%), Postives = 295/304 (97.04%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDG RFVIEA EAE+LAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGPRFVIEASEAETLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRS+A+I
Sbjct: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSAAEI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILV    SDSGSDSKP+ YVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNG SLPNESA 
Sbjct: 121 KILV----SDSGSDSKPNDYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGLSLPNESAG 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP
Sbjct: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC
Sbjct: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300

Query: 301 IAAV 305
           IAAV
Sbjct: 301 IAAV 300

BLAST of CSPI01G28030 vs. ExPASy TrEMBL
Match: A0A1S3BQV2 (Cytidine deaminase OS=Cucumis melo OX=3656 GN=LOC103492314 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.3e-158
Identity = 291/304 (95.72%), Postives = 295/304 (97.04%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDG RFVIEA EAE+LAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGPRFVIEASEAETLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRS+A+I
Sbjct: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSAAEI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILV    SDSGSDSKP+ YVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNG SLPNESA 
Sbjct: 121 KILV----SDSGSDSKPNDYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGLSLPNESAG 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP
Sbjct: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC
Sbjct: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300

Query: 301 IAAV 305
           IAAV
Sbjct: 301 IAAV 300

BLAST of CSPI01G28030 vs. ExPASy TrEMBL
Match: A0A6J1JFD6 (Cytidine deaminase OS=Cucurbita maxima OX=3661 GN=LOC111485242 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.2e-139
Identity = 258/304 (84.87%), Postives = 275/304 (90.46%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDG RFVIEA EAES+AKQS LTIPLLLP+LVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGPRFVIEASEAESMAKQSGLTIPLLLPSLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLH SVHAEQFLVTN ALNAESHLN+LAVSAAPCGHCRQFLQEVRS+A+I
Sbjct: 61  FGVNLEFPGLPLHHSVHAEQFLVTNFALNAESHLNFLAVSAAPCGHCRQFLQEVRSAAEI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILVS   S   SDSK + YVPLPQFLPHRFGPYDLL KDVPLLLEPRFNG SL +ESA 
Sbjct: 121 KILVS---SIEESDSKSNDYVPLPQFLPHRFGPYDLLNKDVPLLLEPRFNGLSLSDESAG 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           N+ LCNG HG + EKLKRAALDAANMSHAPYS+CPSGVALMD +G+IY G YMESAAYNP
Sbjct: 181 NHTLCNGAHGSDWEKLKRAALDAANMSHAPYSRCPSGVALMDADGQIYKGSYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQ AIV+Y+AGGG GYERIVAAVLVEKDGV+VKQE  ARLLL+TISPECEF VVHC
Sbjct: 241 SMGPVQTAIVSYVAGGGGGYERIVAAVLVEKDGVDVKQEHTARLLLQTISPECEFRVVHC 300

Query: 301 IAAV 305
           +AAV
Sbjct: 301 VAAV 301

BLAST of CSPI01G28030 vs. ExPASy TrEMBL
Match: A0A6J1HAV6 (Cytidine deaminase OS=Cucurbita moschata OX=3662 GN=LOC111461704 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 6.5e-139
Identity = 257/304 (84.54%), Postives = 275/304 (90.46%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDG RFVIEA EAES+AKQS LTIPLLLP+LVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGPRFVIEASEAESMAKQSGLTIPLLLPSLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLH SVHAEQFLVTN ALNAES LN+LAVSAAPCGHCRQFLQEVRS+A+I
Sbjct: 61  FGVNLEFPGLPLHHSVHAEQFLVTNFALNAESQLNFLAVSAAPCGHCRQFLQEVRSAAEI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILVS   S   SDSK + YVPLPQFLPHRFGPYDLL KDVPLLLEPRFNG SL +ESAE
Sbjct: 121 KILVS---SIEESDSKSNDYVPLPQFLPHRFGPYDLLNKDVPLLLEPRFNGLSLSDESAE 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           N+ LCNG HG + EKLKRAALDAANMSHAPYS+CPSGVAL+D +G+IY G YMESAAYNP
Sbjct: 181 NHTLCNGAHGSDWEKLKRAALDAANMSHAPYSRCPSGVALIDTDGQIYKGSYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQ AIV+Y+AGGG GYERIVAAVLVEKDGV+VKQE  ARLLL+TISPECEF VVHC
Sbjct: 241 SMGPVQTAIVSYVAGGGGGYERIVAAVLVEKDGVDVKQEHTARLLLQTISPECEFRVVHC 300

Query: 301 IAAV 305
           +AAV
Sbjct: 301 VAAV 301

BLAST of CSPI01G28030 vs. NCBI nr
Match: XP_004135706.1 (cytidine deaminase 1 [Cucumis sativus] >KGN66229.1 hypothetical protein Csa_006974 [Cucumis sativus])

HSP 1 Score: 597.4 bits (1539), Expect = 6.8e-167
Identity = 302/304 (99.34%), Postives = 303/304 (99.67%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI
Sbjct: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNG SLPNE+AE
Sbjct: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGLSLPNETAE 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP
Sbjct: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC
Sbjct: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300

Query: 301 IAAV 305
           IAAV
Sbjct: 301 IAAV 304

BLAST of CSPI01G28030 vs. NCBI nr
Match: XP_008450845.1 (PREDICTED: cytidine deaminase 1-like [Cucumis melo] >TYK10103.1 cytidine deaminase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 568.9 bits (1465), Expect = 2.6e-158
Identity = 291/304 (95.72%), Postives = 295/304 (97.04%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDG RFVIEA EAE+LAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGPRFVIEASEAETLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRS+A+I
Sbjct: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSAAEI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILV    SDSGSDSKP+ YVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNG SLPNESA 
Sbjct: 121 KILV----SDSGSDSKPNDYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGLSLPNESAG 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP
Sbjct: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC
Sbjct: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300

Query: 301 IAAV 305
           IAAV
Sbjct: 301 IAAV 300

BLAST of CSPI01G28030 vs. NCBI nr
Match: XP_038878430.1 (cytidine deaminase 1-like [Benincasa hispida])

HSP 1 Score: 513.5 bits (1321), Expect = 1.3e-141
Identity = 265/304 (87.17%), Postives = 277/304 (91.12%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDG RFVIEAPE ES+AKQS  TIPLLLP+LVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGCRFVIEAPEVESMAKQSGRTIPLLLPSLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLHQSVHAEQFLVTNLALN ESHLNYLAVSA PCGHCRQFLQEVRS+A+I
Sbjct: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNGESHLNYLAVSAFPCGHCRQFLQEVRSAAEI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILVS+      SDS  + YVPLPQFLP+RFGP DLL KDVPLLLEPRFNG +L NESA 
Sbjct: 121 KILVSE------SDSSSNDYVPLPQFLPNRFGPDDLLDKDVPLLLEPRFNGLTLSNESAG 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           N KLCNG HG +LEKLKRAALDAANMSHAPYSKCPSGVALMD+NGRIYNG YMESAAYNP
Sbjct: 181 NEKLCNGTHGGDLEKLKRAALDAANMSHAPYSKCPSGVALMDNNGRIYNGSYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQAAIV+YIAGGG  YERIVAAVLVEKDGVEVKQE+AARLLLETISPECEF VVHC
Sbjct: 241 SMGPVQAAIVSYIAGGGGEYERIVAAVLVEKDGVEVKQEQAARLLLETISPECEFRVVHC 298

Query: 301 IAAV 305
           IAAV
Sbjct: 301 IAAV 298

BLAST of CSPI01G28030 vs. NCBI nr
Match: XP_022987806.1 (cytidine deaminase 1-like [Cucurbita maxima])

HSP 1 Score: 505.0 bits (1299), Expect = 4.6e-139
Identity = 258/304 (84.87%), Postives = 275/304 (90.46%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDG RFVIEA EAES+AKQS LTIPLLLP+LVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGPRFVIEASEAESMAKQSGLTIPLLLPSLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLH SVHAEQFLVTN ALNAESHLN+LAVSAAPCGHCRQFLQEVRS+A+I
Sbjct: 61  FGVNLEFPGLPLHHSVHAEQFLVTNFALNAESHLNFLAVSAAPCGHCRQFLQEVRSAAEI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILVS   S   SDSK + YVPLPQFLPHRFGPYDLL KDVPLLLEPRFNG SL +ESA 
Sbjct: 121 KILVS---SIEESDSKSNDYVPLPQFLPHRFGPYDLLNKDVPLLLEPRFNGLSLSDESAG 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           N+ LCNG HG + EKLKRAALDAANMSHAPYS+CPSGVALMD +G+IY G YMESAAYNP
Sbjct: 181 NHTLCNGAHGSDWEKLKRAALDAANMSHAPYSRCPSGVALMDADGQIYKGSYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQ AIV+Y+AGGG GYERIVAAVLVEKDGV+VKQE  ARLLL+TISPECEF VVHC
Sbjct: 241 SMGPVQTAIVSYVAGGGGGYERIVAAVLVEKDGVDVKQEHTARLLLQTISPECEFRVVHC 300

Query: 301 IAAV 305
           +AAV
Sbjct: 301 VAAV 301

BLAST of CSPI01G28030 vs. NCBI nr
Match: XP_023516485.1 (cytidine deaminase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.8 bits (1296), Expect = 1.0e-138
Identity = 256/304 (84.21%), Postives = 276/304 (90.79%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MDG RFVIEA EAES+AKQS LTIPLLLP+LVKSAQSLARPPISKYHVGAVGLGSSGRVF
Sbjct: 1   MDGPRFVIEASEAESMAKQSGLTIPLLLPSLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
           FGVNLEFPGLPLH SVHAEQFLVTN ALNAESHLN+LAVSAAPCGHCRQFLQEV S+A+I
Sbjct: 61  FGVNLEFPGLPLHHSVHAEQFLVTNFALNAESHLNFLAVSAAPCGHCRQFLQEVSSAAEI 120

Query: 121 KILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAE 180
           KILVS   S   SDSK + YVPLPQFLPHRFGPYDLL KDVPL+LEPRFNG SL +ESAE
Sbjct: 121 KILVS---SIEESDSKSNDYVPLPQFLPHRFGPYDLLNKDVPLILEPRFNGLSLSDESAE 180

Query: 181 NNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNP 240
           N+ LCNG HG + EKLKRAALDAANMSHAPYS+CPSGVAL+D +G+IY G YMESAAYNP
Sbjct: 181 NHTLCNGAHGSDWEKLKRAALDAANMSHAPYSRCPSGVALIDTDGQIYKGSYMESAAYNP 240

Query: 241 SMGPVQAAIVAYIAGGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 300
           SMGPVQ AIV+Y+AGGG GYERIVAAVLVEKDGV+VKQE+ ARLLL+TISPECEF VVHC
Sbjct: 241 SMGPVQTAIVSYVAGGGGGYERIVAAVLVEKDGVDVKQEQTARLLLQTISPECEFRVVHC 300

Query: 301 IAAV 305
           +AAV
Sbjct: 301 VAAV 301

BLAST of CSPI01G28030 vs. TAIR 10
Match: AT2G19570.1 (cytidine deaminase 1 )

HSP 1 Score: 362.5 bits (929), Expect = 3.4e-100
Identity = 191/306 (62.42%), Postives = 231/306 (75.49%), Query Frame = 0

Query: 1   MDGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVF 60
           MD   FVI++ EAES AKQ  +++  LLP+LVK AQS AR PISK++V  VGLGSSGR+F
Sbjct: 1   MDKPSFVIQSKEAESAAKQLGVSVIQLLPSLVKPAQSYARTPISKFNVAVVGLGSSGRIF 60

Query: 61  FGVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSAAPCGHCRQFLQEVRSSADI 120
            GVN+EFP LPLH S+HAEQFLVTNL LN E HLN+ AVSAAPCGHCRQFLQE+R + +I
Sbjct: 61  LGVNVEFPNLPLHHSIHAEQFLVTNLTLNGERHLNFFAVSAAPCGHCRQFLQEIRDAPEI 120

Query: 121 KILVSDSGSDSGSDSKPDV--YVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNES 180
           KIL++D  + + SDS  D   ++ L  FLPHRFGP DLL KD PLLLE   N   +    
Sbjct: 121 KILITDPNNSADSDSAADSDGFLRLGSFLPHRFGPDDLLGKDHPLLLESHDNHLKI---- 180

Query: 181 AENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAY 240
           ++ + +CNGN   + + LK+ AL AAN S+APYS CPSGV+L+D +G++Y G YMESAAY
Sbjct: 181 SDLDSICNGNTDSSAD-LKQTALAAANRSYAPYSLCPSGVSLVDCDGKVYRGWYMESAAY 240

Query: 241 NPSMGPVQAAIVAYIA-GGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTV 300
           NPSMGPVQAA+V Y+A GGG GYERIV AVLVEK+   V+QE  ARLLLETISP+CEF V
Sbjct: 241 NPSMGPVQAALVDYVANGGGGGYERIVGAVLVEKEDAVVRQEHTARLLLETISPKCEFKV 300

Query: 301 VHCIAA 304
            HC  A
Sbjct: 301 FHCYEA 301

BLAST of CSPI01G28030 vs. TAIR 10
Match: AT4G29600.1 (Cytidine/deoxycytidylate deaminase family protein )

HSP 1 Score: 268.1 bits (684), Expect = 8.8e-72
Identity = 158/309 (51.13%), Postives = 197/309 (63.75%), Query Frame = 0

Query: 2   DGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFF 61
           D  +FV  A EA S      +T P+ LP L++ A SLAR PISKY VGAVG  SSGRV+ 
Sbjct: 5   DKYKFVFTAKEAAS----EGVTEPIRLPKLIRKAMSLARGPISKYKVGAVGRASSGRVYL 64

Query: 62  GVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVS--------AAPCGHCRQFLQE 121
           GVN+EFPGLPLH S+H EQFLVTNLALN+E  L  LAV+         APCG+CRQFL E
Sbjct: 65  GVNVEFPGLPLHHSIHPEQFLVTNLALNSEKGLRQLAVAISSDCIEFGAPCGNCRQFLME 124

Query: 122 VRSSADIKILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFS 181
             +  DIKIL+       GS S       L   LP+RF P D+L K  PLLLE R N  +
Sbjct: 125 TSNELDIKILLKSKHEAEGSFSS------LKLLLPYRFTPDDVLPKGSPLLLEKRDNCLT 184

Query: 182 LPNESAENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYM 241
           L   S    ++C+     +   LK  AL AAN S +PY++ PSGVAL DD G+ Y G Y+
Sbjct: 185 L---SGSTEEICS----SDCSHLKCKALAAANNSFSPYTESPSGVALQDDEGKWYRGWYI 244

Query: 242 ESAAYNPSMGPVQAAIVAYIA-GGGAGYERIVAAVLVEKDGVEVKQERAARLLLETI-SP 301
           ES AY+PS+GPVQAA+V ++A   G G+ +IV AVLVEK+   V QER A+++L+TI +P
Sbjct: 245 ESVAYSPSLGPVQAALVDFVARSRGKGFNKIVEAVLVEKNNARVSQERTAKMILDTIAAP 296

BLAST of CSPI01G28030 vs. TAIR 10
Match: AT4G29610.1 (Cytidine/deoxycytidylate deaminase family protein )

HSP 1 Score: 250.4 bits (638), Expect = 1.9e-66
Identity = 143/293 (48.81%), Postives = 187/293 (63.82%), Query Frame = 0

Query: 15  SLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFFGVNLEFPGLPLHQ 74
           S A +  +  P  LP L+  A SLAR P+S + VGAVGL SSG VF GVN+EFP LPLH 
Sbjct: 8   SEAAEEGVRGPSDLPKLIDKAMSLARAPVSTFKVGAVGLTSSGEVFLGVNVEFPNLPLHH 67

Query: 75  SVHAEQFLVTNLALNAESHLNYLAVS------AAPCGHCRQFLQEVRSSADIKILVSDSG 134
           ++HAEQFLVTNLALN+   L ++AVS       APCGHCRQF QE+R++ +I+IL+    
Sbjct: 68  TIHAEQFLVTNLALNSMKKLTHIAVSVTGTIFGAPCGHCRQFYQEMRNAPEIEILIKRPK 127

Query: 135 SDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNESAENNKLCNGN 194
                    D ++ L   +P RFGP  +L +D  LLLE R N   L    ++  ++C  +
Sbjct: 128 DGI------DEFMSLKSLMPERFGPDSILPEDASLLLEQRDNSLVL----SDPEEIC--S 187

Query: 195 HGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAYNPSMGPVQAA 254
             E+    K  AL AAN S+APYSKCPSGVAL+   G +Y G Y+ES AYNPS+GPV+AA
Sbjct: 188 DPEDCSHTKCRALAAANKSYAPYSKCPSGVALI-CGGEVYKGWYIESVAYNPSLGPVEAA 247

Query: 255 IVAYIA-GGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPECEFTVVHC 301
           +V ++A GGG  +  I   VLVE   V+V QE  AR  L+ I+P+C+F V+HC
Sbjct: 248 LVDFVARGGGKEFNEITEVVLVEMKDVKVSQEATARTFLDKIAPKCDFKVLHC 287

BLAST of CSPI01G28030 vs. TAIR 10
Match: AT4G29620.1 (Cytidine/deoxycytidylate deaminase family protein )

HSP 1 Score: 242.7 bits (618), Expect = 4.0e-64
Identity = 146/308 (47.40%), Postives = 193/308 (62.66%), Query Frame = 0

Query: 2   DGTRFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFF 61
           D  +FV  A E    A    +T P+ LP L++ A  LAR PISKY VGAVG  SSGRV+ 
Sbjct: 26  DRFKFVFTANE----AALEGVTDPIRLPNLIRKAMCLARAPISKYKVGAVGRASSGRVYL 85

Query: 62  GVNLEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVS--------AAPCGHCRQFLQE 121
           GVN++FPGLPLH S+HAEQFLVTNLALN E  L  LAV+          PCG+C QFL E
Sbjct: 86  GVNVDFPGLPLHHSIHAEQFLVTNLALNYEKDLCKLAVAISTDGLEFGTPCGNCLQFLME 145

Query: 122 VRSSADIKILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFS 181
           + ++ D+KIL S    ++GS S   + +P            ++L K  P LLE R+N  +
Sbjct: 146 MSNALDMKIL-SKPKHEAGSFSSLRLLLP------------NVLPKGSPFLLEKRYNCLT 205

Query: 182 LPNESAENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYM 241
           L   + E   +C+     +   LKR AL AAN S +PY++ PSGVAL+D++G  Y G Y+
Sbjct: 206 LSGSAGE---ICS----LDCSHLKRRALAAANNSFSPYTESPSGVALLDNDGNWYRGWYI 265

Query: 242 ESAAYNPSMGPVQAAIVAYIA-GGGAGYERIVAAVLVEKDGVEVKQERAARLLLETISPE 301
           ES A NPS+GPVQAA+V ++A   G  + +IV AVLVEK+   V QER A+++L+TI+P 
Sbjct: 266 ESVASNPSLGPVQAALVDFVARSRGKMFNKIVQAVLVEKNNASVSQERTAKIILDTIAPN 309

BLAST of CSPI01G28030 vs. TAIR 10
Match: AT4G29570.1 (Cytidine/deoxycytidylate deaminase family protein )

HSP 1 Score: 212.2 bits (539), Expect = 5.7e-55
Identity = 138/304 (45.39%), Postives = 181/304 (59.54%), Query Frame = 0

Query: 5   RFVIEAPEAESLAKQSALTIPLLLPTLVKSAQSLARPPISKYHVGAVGLGSSGRVFFGVN 64
           RF++   E ES     A T P  L  L+  A    R  IS   V AVG GSSGR FFGVN
Sbjct: 6   RFMLNHIETES---YGAFT-PQNLSPLINRAIPHTRAQISGSPVVAVGRGSSGRTFFGVN 65

Query: 65  LEFPGLPLHQSVHAEQFLVTNLALNAESHLNYLAVSA------APCGHCRQFLQEVRSSA 124
           +E PGLPL  S+HAEQFL+ NLAL+ E  L  +A+S        PCGHC Q L ++R  +
Sbjct: 66  VELPGLPLDHSIHAEQFLLANLALHFEQKLECIAISTNGYYFQEPCGHCCQLLHKIRDMS 125

Query: 125 DIKILVSDSGSDSGSDSKPDVYVPLPQFLPHRFGPYDLLAKDVPLLLEPRFNGFSLPNES 184
           D KIL+++     G+      Y+ L  FLP       +   +VP LLE  FN   L N S
Sbjct: 126 DTKILLTNPTGQKGT------YMNLSTFLPQGL----ISQANVPRLLERNFNCIELINHS 185

Query: 185 AENNKLCNGNHGENLEKLKRAALDAANMSHAPYSKCPSGVALMDDNGRIYNGPYMESAAY 244
              + +C  ++ E+   L   AL AA +S+AP SKCPSGVAL+D  G++Y+G YMES A+
Sbjct: 186 LYMD-IC--SYSEHCNHLNCRALKAATISYAPDSKCPSGVALIDHRGKVYSGGYMESVAH 245

Query: 245 NPSMGPVQAAIVAYIAGG-GAGYERIVAAVLVEKDGVEVKQERAARLLLETIS-PECEFT 301
           N S+GPVQAA+V ++A G G  ++ IV AVLVEK    + QE  AR++LE I+ P+C F 
Sbjct: 246 NTSLGPVQAALVDFVANGDGQEFKNIVEAVLVEKKCGVLSQEATARMILEKIADPDCIFR 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O658964.8e-9962.42Cytidine deaminase 1 OS=Arabidopsis thaliana OX=3702 GN=CDA1 PE=1 SV=1[more]
Q9SU871.2e-7051.13Cytidine deaminase 7 OS=Arabidopsis thaliana OX=3702 GN=CDA7 PE=2 SV=1[more]
Q9SU862.7e-6548.81Cytidine deaminase 6 OS=Arabidopsis thaliana OX=3702 GN=CDA6 PE=3 SV=1[more]
O655715.6e-6347.40Cytidine deaminase 2 OS=Arabidopsis thaliana OX=3702 GN=CDA2 PE=2 SV=1[more]
Q9XEX48.0e-5445.39Cytidine deaminase 8 OS=Arabidopsis thaliana OX=3702 GN=CDA8 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LYU53.3e-16799.34Cytidine deaminase OS=Cucumis sativus OX=3659 GN=Csa_1G587400 PE=3 SV=1[more]
A0A5D3CE071.3e-15895.72Cytidine deaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00... [more]
A0A1S3BQV21.3e-15895.72Cytidine deaminase OS=Cucumis melo OX=3656 GN=LOC103492314 PE=3 SV=1[more]
A0A6J1JFD62.2e-13984.87Cytidine deaminase OS=Cucurbita maxima OX=3661 GN=LOC111485242 PE=3 SV=1[more]
A0A6J1HAV66.5e-13984.54Cytidine deaminase OS=Cucurbita moschata OX=3662 GN=LOC111461704 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135706.16.8e-16799.34cytidine deaminase 1 [Cucumis sativus] >KGN66229.1 hypothetical protein Csa_0069... [more]
XP_008450845.12.6e-15895.72PREDICTED: cytidine deaminase 1-like [Cucumis melo] >TYK10103.1 cytidine deamina... [more]
XP_038878430.11.3e-14187.17cytidine deaminase 1-like [Benincasa hispida][more]
XP_022987806.14.6e-13984.87cytidine deaminase 1-like [Cucurbita maxima][more]
XP_023516485.11.0e-13884.21cytidine deaminase 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G19570.13.4e-10062.42cytidine deaminase 1 [more]
AT4G29600.18.8e-7251.13Cytidine/deoxycytidylate deaminase family protein [more]
AT4G29610.11.9e-6648.81Cytidine/deoxycytidylate deaminase family protein [more]
AT4G29620.14.0e-6447.40Cytidine/deoxycytidylate deaminase family protein [more]
AT4G29570.15.7e-5545.39Cytidine/deoxycytidylate deaminase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002125Cytidine and deoxycytidylate deaminase domainPFAMPF00383dCMP_cyt_deam_1coord: 35..115
e-value: 3.3E-10
score: 39.7
IPR002125Cytidine and deoxycytidylate deaminase domainPROSITEPS51747CYT_DCMP_DEAMINASES_2coord: 23..154
score: 20.079088
IPR002125Cytidine and deoxycytidylate deaminase domainPROSITEPS51747CYT_DCMP_DEAMINASES_2coord: 191..304
score: 23.048012
IPR006263Cytidine deaminase, homodimericTIGRFAMTIGR01355TIGR01355coord: 5..300
e-value: 1.1E-109
score: 364.0
NoneNo IPR availableGENE3D3.40.140.10Cytidine Deaminase, domain 2coord: 5..162
e-value: 4.4E-34
score: 119.6
NoneNo IPR availablePIRSFPIRSF006334Cdd_plus_pseudocoord: 1..302
e-value: 1.2E-101
score: 337.7
NoneNo IPR availableGENE3D3.40.140.10Cytidine Deaminase, domain 2coord: 188..298
e-value: 1.7E-29
score: 104.2
NoneNo IPR availablePANTHERPTHR11644:SF25BNAA03G49610D PROTEINcoord: 1..299
NoneNo IPR availablePANTHERPTHR11644CYTIDINE DEAMINASEcoord: 1..299
NoneNo IPR availableCDDcd01283cytidine_deaminasecoord: 198..265
e-value: 1.95094E-8
score: 49.647
NoneNo IPR availableCDDcd01283cytidine_deaminasecoord: 39..124
e-value: 1.21938E-18
score: 77.7666
IPR013171Cytidine/deoxycytidylate deaminase, zinc-binding domainPFAMPF08211dCMP_cyt_deam_2coord: 144..283
e-value: 4.2E-50
score: 169.4
IPR016192APOBEC/CMP deaminase, zinc-bindingPROSITEPS00903CYT_DCMP_DEAMINASES_1coord: 77..111
IPR016193Cytidine deaminase-likeSUPERFAMILY53927Cytidine deaminase-likecoord: 142..297
IPR016193Cytidine deaminase-likeSUPERFAMILY53927Cytidine deaminase-likecoord: 11..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G28030.1CSPI01G28030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009972 cytidine deamination
cellular_component GO:0005829 cytosol
molecular_function GO:0004126 cytidine deaminase activity
molecular_function GO:0047844 deoxycytidine deaminase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016787 hydrolase activity