CSPI01G27320 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G27320
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Asparagine--tRNA ligase
Location	Chr1: 22197019 .. 22200180 (+)
RNA-Seq Expression	CSPI01G27320
Synteny	CSPI01G27320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACCATTTAAAATAACTGAGTATTTTTTCATTGTCTTTTCTTGTGGGGAAAGAAAATTTTTGAGAGAAAAACGAACCGGACCAAATTGAGAACCGAACCGGCGCGAACCTGTACGTGGACTTTAAATACAAAGCGTGGATTAGGGTTTAGAATTCAACTGCTGAAGTTGAGACATTCACCGGGAACGGACCAATGGCAGAGCACTCCGCGCCACCCACTGACCAACTCGCCGCCGTCGCCTTGAACAACGATGCTCCCGCTTACGAATCTCTTAAAGCTGAATTCTCTGACCGTGTTACGATCCGTACAATTGTCTCCCGGCCCGACGGCGGCGCAGGCCTCGCCGGCCAGAAAGTACGCGTCGGTGGTTGGGTCAAAACTGGAAGAAAGGCTGATAAGGACGCTTTTGCTTTTCTCGAGTTGAACGACGGGTCTTGCCCACAGAATCTTCAAGTGATCATCGAAGCGTCTGTGGCGGATCTTGGCCAGATTGTTCCCACTGGAACCTGCGTTGTTGTTGACGGCGTCTTCAAGCTTCCTCCTCCGGGTAAGCACCAGAAAGTTGAACTTCGGGCTGATGCTGTCGTTCATGTCGGTCCTGTTGATCCCGCTCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAATTTCTCAGAGATGTTGTTCATCTCCGCCCTAGAACTAACACGGTAAGAGAAATCATTCTCGTCTCTCGAAAATGAATTTAGTAACTGATTCAAATCTCTCCATTGGGTTTTTTTTTTTCATATTTTTCGATTTCATTTCTTATATTGTTCTGTAACTTAACATTTAAACAGGAAAGGCACAATTTTTATTCACGGGTTTATGTTTCTTCTATGATTACAGATATCTGCAGTTGCAAGAATTCGAAATGCACTTGCATATGCCACTCATACATTTTTTAACAACCATGGATTTCTGTATATTCACACACCGATTGTTACCACAAGCGATTGTGAAGGTGCTGGTGAAATGTTTCAAGTTACTACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAAAACCCACCTCCATCAGAGGCAGAGGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAGTCTGAAGGCTGCTAAAGCCAGCAAGGAGGAAATTGGTGCTTCTGTTTTGGAACTGAAGAAGGCAAAGGAGAATGTTGCTAGATTGGAAGAGAGAGCAAAGCTTAAACCAGGCATTCCAGAGAAAGATGGGAAAGTTGATTACACCAAGGATTTCTTCGCTCGGCAAGCGTTCTTGACTGTTTCGGGTCAGCTGCAAGTTGAAACTTATGCCTGTGCCCTAAGTAGTGTATACACCTTTGGACCGACCTTCCGCGCTGAAAACTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGTTTGTTACTTTGCTGAAATTTTGTTCCAAGTTGTATTTTTTGCTCTGCGTACTCAGTTGGAATTGCTTAGAAGATTAATCATTTACTATAACCATCATTAGATACAATACCTCTGACATTTATCTGGTAGTGTGGGCTTTGTGTTCTAGGATGAGTTCATCTTCTTTTTTAGTTTTCTAATTAACTTCTATACGGAATTGATTTACGTTGGCCTAGTGATCATTTGTAAAGAAATGAATTAAAAAAACGATTTCAAGCCATAGAGTCCACTTGCTCACGGTTCTGAAAGCGAAAAACAAAAAAAAGGTCTTCTAATTTATTAGTTGGAGTCCTACTTGATATATTTTTCATATGAACTCTAATTGAGAAGAAACTGCTAGAATGCTAGGTTTAAGCTTTCTTTTTCATCTGTACTACCAAGCTAAATGGCAATCAACCATGTTTAAGAGAGCTTGGTTTGGAAATTCTGGGATGAATAAGAAAAACAACAGAACTTTGCAAATAGGGTTTTTGTTCTCTTTGGTTGATTGTATTCTCTTCTTCTAGAATTTTAAATTAATTAACTGAAAATGAGATCTGTTTCTTCAATTGTTGAAGGACGATATGAATTGTGCGGAAGCCTATGTGAGATTTATGTGTCAATGGTTACTTGACAACTGCATAGATGACATGAAATTTATGGCTGAAAAGTATGACCGAACTTGTATTGATCGTCTCAAAATGGTCTCTTCAACCCCTTTCGTTCGAGTCTCCTATACAGAAGCAGTTGAACTACTTGAGGAAGCCGTAAAGAAGGGCAAGGAATTCGAGAACAAAGTGGAATGGGGTATTGATTTAGCTTCAGAGCATGAAAGGTTTGTGAACTACCTATCATTCTTTTCATTTACATTCCACTTGAACAATGGTGATTAATCTCCTCACTTTTTTCTGCTTTCTTTCTCAGATACTTGACTGAGGTTGTATTTAAAAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGACTCAATGACGACATGAAAACGGTAGCTGCAATGGATGTTCTTGTACCTAAGGTAGTAGCTTATTTGGTTTTCACTATTTCTTTAATGGCTTTGATTTGTTTCACCATCTTGATCTGTTCGTAAAAATTGAAAAATTTCCTCTGTTGAAGGTTGGGGAATTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGTGACAGGTAGAAGAACAATACATAGTTGTGTTTTCTGGTTGGTTCTCCCCATTTCTCTTTTGAACTATGAAATTCAAATTCTCACTTCCTGCAGGATCAAGGAGATGGGTCTGCCACTTGAACCATATGAATGGTACCTTGATCTGAGACGATACGGGACAGTCAAGCACTGTGGTTTTGGTCTCGGTTTCGAGCGGATGCTCCTTTTCGCCACTGGCATTGACAACATCAGAGACGTTATTCCCTTCCCCAGATATCCAGGACGAGCAGACCTATAATTACACAACACAAGATCTTAAGTTCCCTTTCTACCTGATGAAACTAGAGACCTTCGAATGAGAAGATTTGATTGTTTGTATTATACTTATCTTTCAAATTGTTGGGTTTTAATTTATCCTCAGAAATTTTGGATTGGTGGGTTTGTTTTATATATAGCACAATACTCTTTTGCATTTGAAATGACTTCAATTGGTTTCA

mRNA sequence

AAACCATTTAAAATAACTGAGTATTTTTTCATTGTCTTTTCTTGTGGGGAAAGAAAATTTTTGAGAGAAAAACGAACCGGACCAAATTGAGAACCGAACCGGCGCGAACCTGTACGTGGACTTTAAATACAAAGCGTGGATTAGGGTTTAGAATTCAACTGCTGAAGTTGAGACATTCACCGGGAACGGACCAATGGCAGAGCACTCCGCGCCACCCACTGACCAACTCGCCGCCGTCGCCTTGAACAACGATGCTCCCGCTTACGAATCTCTTAAAGCTGAATTCTCTGACCGTGTTACGATCCGTACAATTGTCTCCCGGCCCGACGGCGGCGCAGGCCTCGCCGGCCAGAAAGTACGCGTCGGTGGTTGGGTCAAAACTGGAAGAAAGGCTGATAAGGACGCTTTTGCTTTTCTCGAGTTGAACGACGGGTCTTGCCCACAGAATCTTCAAGTGATCATCGAAGCGTCTGTGGCGGATCTTGGCCAGATTGTTCCCACTGGAACCTGCGTTGTTGTTGACGGCGTCTTCAAGCTTCCTCCTCCGGGTAAGCACCAGAAAGTTGAACTTCGGGCTGATGCTGTCGTTCATGTCGGTCCTGTTGATCCCGCTCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAATTTCTCAGAGATGTTGTTCATCTCCGCCCTAGAACTAACACGATATCTGCAGTTGCAAGAATTCGAAATGCACTTGCATATGCCACTCATACATTTTTTAACAACCATGGATTTCTGTATATTCACACACCGATTGTTACCACAAGCGATTGTGAAGGTGCTGGTGAAATGTTTCAAGTTACTACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAAAACCCACCTCCATCAGAGGCAGAGGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAGTCTGAAGGCTGCTAAAGCCAGCAAGGAGGAAATTGGTGCTTCTGTTTTGGAACTGAAGAAGGCAAAGGAGAATGTTGCTAGATTGGAAGAGAGAGCAAAGCTTAAACCAGGCATTCCAGAGAAAGATGGGAAAGTTGATTACACCAAGGATTTCTTCGCTCGGCAAGCGTTCTTGACTGTTTCGGGTCAGCTGCAAGTTGAAACTTATGCCTGTGCCCTAAGTAGTGTATACACCTTTGGACCGACCTTCCGCGCTGAAAACTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGACGATATGAATTGTGCGGAAGCCTATGTGAGATTTATGTGTCAATGGTTACTTGACAACTGCATAGATGACATGAAATTTATGGCTGAAAAGTATGACCGAACTTGTATTGATCGTCTCAAAATGGTCTCTTCAACCCCTTTCGTTCGAGTCTCCTATACAGAAGCAGTTGAACTACTTGAGGAAGCCGTAAAGAAGGGCAAGGAATTCGAGAACAAAGTGGAATGGGGTATTGATTTAGCTTCAGAGCATGAAAGATACTTGACTGAGGTTGTATTTAAAAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGACTCAATGACGACATGAAAACGGTAGCTGCAATGGATGTTCTTGTACCTAAGGTTGGGGAATTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGTGACAGGATCAAGGAGATGGGTCTGCCACTTGAACCATATGAATGGTACCTTGATCTGAGACGATACGGGACAGTCAAGCACTGTGGTTTTGGTCTCGGTTTCGAGCGGATGCTCCTTTTCGCCACTGGCATTGACAACATCAGAGACGTTATTCCCTTCCCCAGATATCCAGGACGAGCAGACCTATAATTACACAACACAAGATCTTAAGTTCCCTTTCTACCTGATGAAACTAGAGACCTTCGAATGAGAAGATTTGATTGTTTGTATTATACTTATCTTTCAAATTGTTGGGTTTTAATTTATCCTCAGAAATTTTGGATTGGTGGGTTTGTTTTATATATAGCACAATACTCTTTTGCATTTGAAATGACTTCAATTGGTTTCA

Coding sequence (CDS)

ATGGCAGAGCACTCCGCGCCACCCACTGACCAACTCGCCGCCGTCGCCTTGAACAACGATGCTCCCGCTTACGAATCTCTTAAAGCTGAATTCTCTGACCGTGTTACGATCCGTACAATTGTCTCCCGGCCCGACGGCGGCGCAGGCCTCGCCGGCCAGAAAGTACGCGTCGGTGGTTGGGTCAAAACTGGAAGAAAGGCTGATAAGGACGCTTTTGCTTTTCTCGAGTTGAACGACGGGTCTTGCCCACAGAATCTTCAAGTGATCATCGAAGCGTCTGTGGCGGATCTTGGCCAGATTGTTCCCACTGGAACCTGCGTTGTTGTTGACGGCGTCTTCAAGCTTCCTCCTCCGGGTAAGCACCAGAAAGTTGAACTTCGGGCTGATGCTGTCGTTCATGTCGGTCCTGTTGATCCCGCTCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAATTTCTCAGAGATGTTGTTCATCTCCGCCCTAGAACTAACACGATATCTGCAGTTGCAAGAATTCGAAATGCACTTGCATATGCCACTCATACATTTTTTAACAACCATGGATTTCTGTATATTCACACACCGATTGTTACCACAAGCGATTGTGAAGGTGCTGGTGAAATGTTTCAAGTTACTACTTTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGATTAAAAACCCACCTCCATCAGAGGCAGAGGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAGTCTGAAGGCTGCTAAAGCCAGCAAGGAGGAAATTGGTGCTTCTGTTTTGGAACTGAAGAAGGCAAAGGAGAATGTTGCTAGATTGGAAGAGAGAGCAAAGCTTAAACCAGGCATTCCAGAGAAAGATGGGAAAGTTGATTACACCAAGGATTTCTTCGCTCGGCAAGCGTTCTTGACTGTTTCGGGTCAGCTGCAAGTTGAAACTTATGCCTGTGCCCTAAGTAGTGTATACACCTTTGGACCGACCTTCCGCGCTGAAAACTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGACGATATGAATTGTGCGGAAGCCTATGTGAGATTTATGTGTCAATGGTTACTTGACAACTGCATAGATGACATGAAATTTATGGCTGAAAAGTATGACCGAACTTGTATTGATCGTCTCAAAATGGTCTCTTCAACCCCTTTCGTTCGAGTCTCCTATACAGAAGCAGTTGAACTACTTGAGGAAGCCGTAAAGAAGGGCAAGGAATTCGAGAACAAAGTGGAATGGGGTATTGATTTAGCTTCAGAGCATGAAAGATACTTGACTGAGGTTGTATTTAAAAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGACTCAATGACGACATGAAAACGGTAGCTGCAATGGATGTTCTTGTACCTAAGGTTGGGGAATTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGTGACAGGATCAAGGAGATGGGTCTGCCACTTGAACCATATGAATGGTACCTTGATCTGAGACGATACGGGACAGTCAAGCACTGTGGTTTTGGTCTCGGTTTCGAGCGGATGCTCCTTTTCGCCACTGGCATTGACAACATCAGAGACGTTATTCCCTTCCCCAGATATCCAGGACGAGCAGACCTATAA

Protein sequence

MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGKHQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYATHTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDYTKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAVELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL*

Homology

[Close]

BLAST of CSPI01G27320 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 6.4e-254
Identity = 426/574 (74.22%), Postives = 497/574 (86.59%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MA+   PP  QLAAV+L ND    +  +A+FS+RV IRTI+ RPDGGAGLAGQ VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ S+ D+  +V TGTCV VDGV K+PP GK
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALA 180
              Q++EL    V+ VG VD ++YPLPKTKLTLE LRDV+HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF  H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IV E+G  VA LKAAKASKE I A+V ELK AKE  A ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYTKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+KDFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTE 420
           FADL+DDMNCAEAYV++MC WLL+ C  DM+ MA+ +D  CIDRLK+V+STPF R++YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AVELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           A+ELLEEAV KGKEF+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHC 540
           D KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+EMGLP+EPYEWYLDLRRYGTVKHC
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]

BLAST of CSPI01G27320 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 1.9e-245
Identity = 423/576 (73.44%), Postives = 486/576 (84.38%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           M +   PP +QLAA  L ND    +  KA+FSDRV IR+I+    GGA LAGQKVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSILG---GGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++S+ DL ++V TGTCV VDGV K+PP GK
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALA 180
              Q +EL  + V+ VG VDP  YPLPKTKLT EFLRDV+HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF  H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAVA LK AKASKEEI ASV +L  AK ++A +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYTKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+ DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTE 420
           FAD+ DDMNCAEAYV++MC+WL+D C DDM+ M +  D  C  RL MV+   F RV+YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AVELLEEAVKKGK-EFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           A+E LE+AV +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+EMGLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HCGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           HCGFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]

BLAST of CSPI01G27320 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.0e-142
Identity = 270/541 (49.91%), Postives = 340/541 (62.85%), Query Frame = 0


Query: 34  RVTIRTIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS 93
           R  I+++         L G  + + GWV+T R  ++  F F+E+NDGS   N Q+I    
Sbjct: 2   RTKIKSLRGTTPEVRALIGHDITLKGWVRTVR--NQKTFTFIEINDGSTLSNFQIIATPD 61

Query: 94  VADLGQIV---PTGTCVVVDGVFKLPPPGKHQKVELRADAVVHVGPVDPARYPLPKTKLT 153
           +A   Q++    TG  V   G   +  PGK Q +E++A A+  +G  DP  Y L K + T
Sbjct: 62  IAGYDQLINQLSTGVSVSAIGTI-VESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHT 121

Query: 154 LEFLRDVVHLRPRTNTISAVARIRNALAYATHTFFNNHGFLYIHTPIVTTSDCEGAGEMF 213
            EFLR + HLRPRTNTI AV R+RNALA+ATH FF   GFLYIHTPI+T SDCEGAG+MF
Sbjct: 122 FEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMF 181

Query: 214 QVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVASLKAAKASKEEIGASVLE 273
           QVTTL                                                       
Sbjct: 182 QVTTL------------------------------------------------------- 241

Query: 274 LKKAKENVARLEERAKLKPGIPEKDGKVDYTKDFFARQAFLTVSGQLQVETYACALSSVY 333
               + N AR  E            G+VDYT+DFF +  +LTVSGQL  E YACALS VY
Sbjct: 242 ---DQNNPARTPE------------GRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVY 301

Query: 334 TFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFMCQWLLDNCIDDMKF 393
           TFGPTFRAENS+TSRHLAEFWM+EPE+AFADL D+M+CAE Y++++ +++LDNC +DM+F
Sbjct: 302 TFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEF 361

Query: 394 MAEKYDRTCIDRLKMVSSTPFVRVSYTEAVELLEEAVKKGKEFENKVEWGIDLASEHERY 453
             +      I RL+ V +T F R SYT AV +LE+A KK   FE  V+WG+DL SEHER+
Sbjct: 362 FNKHVATDLISRLEHVINTSFERASYTYAVRILEKADKK---FEYPVKWGLDLQSEHERF 421

Query: 454 LTEVVFKKPVIVYNYPKGIKAFYMRLNDDMKTVAAMDVLVPKVGELIGGSQREERYEVIR 513
           L E  F KPVI+ +YPK IKAFYMR N+D KTVAAMDVLVPKVGE+IGGSQREER  V+ 
Sbjct: 422 LAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLE 466

Query: 514 DRIKEMGLPLEPYEWYLDLRRYGTVKHCGFGLGFERMLLFATGIDNIRDVIPFPRYPGRA 572
            ++KE  LP E Y WYL+LR++G+V H GFG GFER++ F TG++NIRDVIPFPR+PG+A
Sbjct: 482 SKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKA 466

[Close]

BLAST of CSPI01G27320 vs. ExPASy Swiss-Prot
Match: Q7MVE5 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=asnS PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.9e-141
Identity = 260/525 (49.52%), Postives = 331/525 (63.05%), Query Frame = 0


Query: 49  GLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQI--VPTGTC 108
           GL GQ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + +  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPGKHQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNT 168
           + V G   +   G  Q VE++A A+   G  DPA YPL K   TLEFLR++ HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
             A+ RIR+ +A A HTFF+N G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGAIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 IKNPPPSEAEVDEARKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAK 288
             NP                                             +++ R EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYTKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY KDFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F++E +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRVSYTEAVELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR++YTE + +LEEAVK G +FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  Y+ +  R  EMG+P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHCGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  H GFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]

BLAST of CSPI01G27320 vs. ExPASy Swiss-Prot
Match: B2RK42 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) OX=431947 GN=asnS PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 5.6e-141
Identity = 259/525 (49.33%), Postives = 330/525 (62.86%), Query Frame = 0


Query: 49  GLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQI--VPTGTC 108
           GL GQ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + +  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPGKHQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNT 168
           + V G   +   G  Q VE++A A+   G  DPA YPL K   TLEFLR++ HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
              + RIR+ +A A HTFF+N G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGVIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 IKNPPPSEAEVDEARKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAK 288
             NP                                             +++ R EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYTKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY KDFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F++E +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRVSYTEAVELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR++YTE + +LEEAVK G +FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  Y+ +  R  EMG+P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHCGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  H GFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]

BLAST of CSPI01G27320 vs. ExPASy TrEMBL
Match: A0A0A0LYN5 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_1G573730 PE=3 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. ExPASy TrEMBL
Match: A0A5D3CE86 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003180 PE=3 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 565/572 (98.78%), Postives = 568/572 (99.30%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPTDQLAAV LNNDA AYESLKAEFSDRV IRTIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R IVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RNIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAEAYVRFMCQWLLDNC+DDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD+
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. ExPASy TrEMBL
Match: A0A1S3BPW6 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103492241 PE=3 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 565/572 (98.78%), Postives = 568/572 (99.30%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPTDQLAAV LNNDA AYESLKAEFSDRV IRTIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R IVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RNIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAEAYVRFMCQWLLDNC+DDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD+
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. ExPASy TrEMBL
Match: A0A6J1HAP9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 558/572 (97.55%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQLAAV LN+DAP Y SLKAEFSDRV IR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVA LKA+KASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            KDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAE+YVRF+CQWLLDNC+DDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. ExPASy TrEMBL
Match: A0A6J1JKK1 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1)

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 557/572 (97.38%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQLAAV LN+DAP Y SLKAEFSDRV IR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVA LKA+KASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            KDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAEAYVRF+CQWLLDNC+DDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. NCBI nr
Match: XP_004135659.1 (asparagine--tRNA ligase, cytoplasmic 1 [Cucumis sativus] >KGN66154.1 hypothetical protein Csa_006936 [Cucumis sativus])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. NCBI nr
Match: XP_008450753.1 (PREDICTED: asparagine--tRNA ligase, cytoplasmic 1-like [Cucumis melo] >TYK10173.1 asparagine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 565/572 (98.78%), Postives = 568/572 (99.30%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPTDQLAAV LNNDA AYESLKAEFSDRV IRTIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R IVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RNIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAEAYVRFMCQWLLDNC+DDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD+
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. NCBI nr
Match: XP_038879816.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Benincasa hispida])

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 555/572 (97.03%), Postives = 564/572 (98.60%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQLAAV LNNDAP Y+SLKAEFSDRV IRTIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNNDAPTYDSLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADL QIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLSQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVA+LKA+KASKEEIGASVLELKKAKENVA+LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVANLKASKASKEEIGASVLELKKAKENVAKLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            KDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAEAYVRFMCQWLLDNC+DDMKFMAEKYDRTCIDRLKMVSSTPFVR+SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRISYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           E+LEEAVKKGKEFENKVEWGIDLASEHERYLTEV+FKKPVIVYNYPKGIKAFYMRLNDD+
Sbjct: 421 EVLEEAVKKGKEFENKVEWGIDLASEHERYLTEVIFKKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVI DRIKEMGLPLEPYEWYLDLRRYGTVKHCGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIHDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. NCBI nr
Match: XP_022961000.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 558/572 (97.55%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQLAAV LN+DAP Y SLKAEFSDRV IR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVA LKA+KASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            KDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAE+YVRF+CQWLLDNC+DDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. NCBI nr
Match: XP_023520843.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 557/572 (97.38%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MAEH APP DQLAAV LN+DAP Y SLKAEFSDRV IR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHPAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVA LKA+KASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            KDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420
           DLQDDMNCAEAYVRF+CQWLLDNC+DDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of CSPI01G27320 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 877.9 bits (2267), Expect = 4.6e-255
Identity = 426/574 (74.22%), Postives = 497/574 (86.59%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           MA+   PP  QLAAV+L ND    +  +A+FS+RV IRTI+ RPDGGAGLAGQ VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ S+ D+  +V TGTCV VDGV K+PP GK
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALA 180
              Q++EL    V+ VG VD ++YPLPKTKLTLE LRDV+HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF  H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IV E+G  VA LKAAKASKE I A+V ELK AKE  A ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYTKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+KDFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTE 420
           FADL+DDMNCAEAYV++MC WLL+ C  DM+ MA+ +D  CIDRLK+V+STPF R++YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AVELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           A+ELLEEAV KGKEF+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHC 540
           D KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+EMGLP+EPYEWYLDLRRYGTVKHC
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]

BLAST of CSPI01G27320 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 849.7 bits (2194), Expect = 1.3e-246
Identity = 423/576 (73.44%), Postives = 486/576 (84.38%), Query Frame = 0


Query: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60
           M +   PP +QLAA  L ND    +  KA+FSDRV IR+I+    GGA LAGQKVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSILG---GGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++S+ DL ++V TGTCV VDGV K+PP GK
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALA 180
              Q +EL  + V+ VG VDP  YPLPKTKLT EFLRDV+HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF  H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAVA LK AKASKEEI ASV +L  AK ++A +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYTKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+ DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTE 420
           FAD+ DDMNCAEAYV++MC+WL+D C DDM+ M +  D  C  RL MV+   F RV+YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AVELLEEAVKKGK-EFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           A+E LE+AV +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+EMGLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HCGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           HCGFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]

BLAST of CSPI01G27320 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 488.8 bits (1257), Expect = 5.9e-138
Identity = 267/562 (47.51%), Postives = 348/562 (61.92%), Query Frame = 0


Query: 19  NDAPAYE----SLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAF 78
           N   +YE    S   EF  ++ I  +    D G    GQ + + GWV+T R   + +  F
Sbjct: 72  NKVESYEKRFGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIMGWVRTLR--SQSSVTF 131

Query: 79  LELNDGSCPQNLQVIIEASVADLGQI----VPTGTCVVVDGVFKLPPPGKHQKVELRADA 138
           +E+NDGSC  NLQ ++ +      Q+    + TG  V V G   +   G  QKVEL+ + 
Sbjct: 132 IEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTI-VASQGTKQKVELKVEK 191

Query: 139 VVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYATHTFFNNHGF 198
           ++ VG  D + YP+ K +++ EFLR   HLRPRTNT  AVAR+RN LAYATH FF   GF
Sbjct: 192 IIVVGECD-SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGF 251

Query: 199 LYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEA 258
           +++ +PI+T SDCEGAGE F VTTL+                                  
Sbjct: 252 VWVASPIITASDCEGAGEQFCVTTLI---------------------------------- 311

Query: 259 VASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDYTKDFFARQAF 318
                   +S E    S+  + K                    K G +D+++DFF + AF
Sbjct: 312 -------PSSHENTDTSIDAIPKT-------------------KGGLIDWSQDFFGKPAF 371

Query: 319 LTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAE 378
           LTVSGQL  ETYA ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL DDM CA 
Sbjct: 372 LTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACAT 431

Query: 379 AYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAVELLEEAVKKG 438
           AY++++ +++LDNC +DM+F     ++  I RL  V+   F+++ YT+A+E+L   +K  
Sbjct: 432 AYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEIL---LKAN 491

Query: 439 KEFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRLNDDMKTVAAMDVL 498
           K+F+  V+WG+DL SEHERY+TE  F  +PVI+ +YPK IKAFYMR NDD KTVAAMD+L
Sbjct: 492 KKFDFPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDML 551

Query: 499 VPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGFGLGFERMLL 558
           VP++GELIGGSQREER EV+  R+ E+ L  E Y WYLDLRRYG+V H GFGLGFER++ 
Sbjct: 552 VPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQ 566

Query: 559 FATGIDNIRDVIPFPRYPGRAD 572
           F TGIDNIRDVIPFPR P  A+
Sbjct: 612 FVTGIDNIRDVIPFPRTPASAE 566

[Close]

BLAST of CSPI01G27320 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 477.2 bits (1227), Expect = 1.8e-134
Identity = 260/634 (41.01%), Postives = 382/634 (60.25%), Query Frame = 0


Query: 18  NNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDA------ 77
           +ND        +++S RV ++T++ R D GAGLAG++V +GGWVK+ R   K++      
Sbjct: 13  DNDLSPKPITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLP 72

Query: 78  ------------------------------------------------------------ 137
                                                                       
Sbjct: 73  VVAAPSPSSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKA 132

Query: 138 -------FAFLELNDGSCPQNLQVIIEASVADL--GQIVPTGTCVVVDGVFKLP--PPGK 197
                  F FL ++DGS   +LQV+++++++ +   Q++  GTC+V +GV +LP     K
Sbjct: 133 VPPPEYVFYFL-ISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAK 192

Query: 198 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 257
           H  +EL A+ ++HVG VDP +YPL K +L L  LRD  H RPRT T+ +V R+ +AL  A
Sbjct: 193 H-VIELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLA 252

Query: 258 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAE---V 317
           +HTF   HGF Y+  P++TT+   G GEMF+VTTLL + +  E+   K PP  E +   +
Sbjct: 253 SHTFLQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEE---KKPPVQEKDGFSI 312

Query: 318 DEARKIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGK 377
           D  + ++KEK   +  LK + +++E + A+V +LKK  +  +++E + K K G   K  K
Sbjct: 313 DTVKAVIKEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEK 372

Query: 378 VDYTKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEI 437
           +D++KDFF R  +LT SG+  +E+YA AL  VYTFGP F A+    +RHLAE W VE E+
Sbjct: 373 LDFSKDFFGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEM 432

Query: 438 AFADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYT 497
           AFA+L D M+CA+ Y +F+C+++L+N  +DMKF++++ D+T   RL+  +S+  +R SYT
Sbjct: 433 AFAELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYT 492

Query: 498 EAVELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLN 557
           E + LL++A     +FE K EWG+ L +EH  YLT+ ++K PVIV+ YPK IK FY+RLN
Sbjct: 493 EVISLLQKATT--TKFETKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLN 552

Query: 558 DDMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKH 572
           DD KTVAA D++VPKVG +I GSQ EER+E++  RI E G   E +EWYLDLRR+GTVKH
Sbjct: 553 DDKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKH 612

[Close]

BLAST of CSPI01G27320 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 1.2e-26
Identity = 92/291 (31.62%), Postives = 148/291 (50.86%), Query Frame = 0


Query: 286 KLKPGIPEKDGKVDYTKDFFARQAFLTVSGQLQVETYACA-LSSVYTFGPTFRAENSHTS 345
           KL  G  E  G   +  ++  + A L  S QL  +   C  L  V+  GP FRAE+S T 
Sbjct: 279 KLMAGSSE-GGSAVFRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTH 338

Query: 346 RHLAEFWMVEPEIAF-ADLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRL 405
           RHL EF  ++ E+       + M+  +    F+   L + C  +++ + ++Y     + L
Sbjct: 339 RHLCEFVGLDVEMEIRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYP---FEPL 398

Query: 406 KMVSSTPFVRVSYTEAVELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKP---- 465
           K +  T  +R+++ E V++L+EA   G E    V+   DL +E ER L ++V +K     
Sbjct: 399 KFLPKT--LRLTFEEGVQMLKEA---GVE----VDPLGDLNTESERKLGQLVLEKYNTEF 458

Query: 466 VIVYNYPKGIKAFY-MRLNDDMKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGL 525
            I++ YPK ++ FY M   D+     + DV + +  E+I G+QR    EV+  R  E G+
Sbjct: 459 YILHRYPKAVRPFYTMTCADNPLYSNSFDVFI-RGEEIISGAQRVHIPEVLEQRAGECGI 518

Query: 526 PLEPYEWYLDLRRYGTVKHCGFGLGFERMLLFATGIDNIRDVIPFPRYPGR 570
            ++    Y+D  RYG   H GFG+G ER+++    ++NIR    FPR P R
Sbjct: 519 DVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQR 555

The following BLAST results are available for this feature:

BLAST of CSPI01G27320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SW96	6.4e-254	74.22	Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ...	[more]
Q9SSK1	1.9e-245	73.44	Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ...	[more]
Q6MEC9	1.0e-142	49.91	Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G...	[more]
Q7MVE5	1.9e-141	49.52	Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) ...	[more]
B2RK42	5.6e-141	49.33	Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 207...	[more]

BLAST of CSPI01G27320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYN5	0.0e+00	100.00	Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_1G573730 PE=3 SV=1	[more]
A0A5D3CE86	0.0e+00	98.78	Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3BPW6	0.0e+00	98.78	Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103492241 PE=3 SV=1	[more]
A0A6J1HAP9	0.0e+00	94.58	Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1	[more]
A0A6J1JKK1	0.0e+00	94.58	Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1	[more]

BLAST of CSPI01G27320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135659.1	0.0e+00	100.00	asparagine--tRNA ligase, cytoplasmic 1 [Cucumis sativus] >KGN66154.1 hypothetica...	[more]
XP_008450753.1	0.0e+00	98.78	PREDICTED: asparagine--tRNA ligase, cytoplasmic 1-like [Cucumis melo] >TYK10173....	[more]
XP_038879816.1	0.0e+00	97.03	asparagine--tRNA ligase, cytoplasmic 1-like [Benincasa hispida]	[more]
XP_022961000.1	0.0e+00	94.58	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 A...	[more]
XP_023520843.1	0.0e+00	94.58	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI01G27320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56680.1	4.6e-255	74.22	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G70980.1	1.3e-246	73.44	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	5.9e-138	47.51	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT3G07420.1	1.8e-134	41.01	asparaginyl-tRNA synthetase 2	[more]
AT4G31180.1	1.2e-26	31.62	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 264..284
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 143..571 e-value: 1.5E-108 score: 365.1
None	No IPR available	GENE3D	2.40.50.140		coord: 28..138 e-value: 3.5E-10 score: 41.6
None	No IPR available	PANTHER	PTHR22594:SF52	BNAC03G13340D PROTEIN	coord: 1..572
None	No IPR available	PANTHER	PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE	coord: 1..572
None	No IPR available	CDD	cd00776	AsxRS_core	coord: 148..568 e-value: 9.21011E-144 score: 416.197
None	No IPR available	CDD	cd04318	EcAsnRS_like_N	coord: 54..135 e-value: 2.12058E-25 score: 98.0212
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 132..571
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 527..541 score: 46.21 coord: 327..340 score: 43.83 coord: 487..503 score: 44.79 coord: 311..323 score: 40.73
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 301..565 e-value: 5.1E-63 score: 213.0
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 153..214 e-value: 1.1E-11 score: 44.4
IPR004522	Asparagine-tRNA ligase	TIGRFAM	TIGR00457	TIGR00457	coord: 49..571 e-value: 1.4E-168 score: 559.3
IPR004522	Asparagine-tRNA ligase	HAMAP	MF_00534	Asn_tRNA_synth	coord: 37..572 score: 42.14632
IPR000738	WHEP-TRS domain	PROSITE	PS51185	WHEP_TRS_2	coord: 236..292 score: 10.162921
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 329..562 score: 13.378044
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 48..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G27320.1	CSPI01G27320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006421	asparaginyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004816	asparagine-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding