CSPI01G26690 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G26690
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionamidase 1-like
LocationChr1: 21772587 .. 21776899 (-)
RNA-Seq ExpressionCSPI01G26690
SyntenyCSPI01G26690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAGATCAGCTCTGCAATTTCTGGCCATTATTAGGGCGGTTGTGGTGGAGTCTGATTAGGGAGAGCGATCTTAGGCTTTTGCAATGGCTGTGGATGATTATGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCTTCCGATAAACTTCCCTTAAGTGGACTCACTTTCGCTGTTAAAGACATGTGAGTTTCTGATTTTTTACTGTTTAAGTTCATCAAGTCTTTGGTGATCAATAGTCTGTGGAATCTGAAATATCTGTATAGAATCAAAAGATTTCAAGAACAGTTTTGAAATATTGTTACTGTTATATTTGTAGATATTGATGTTTCCATTTTTACAAGCCATCAACTGTTGGTTTTTCTATTTCAATGGAGCTTAGATCGATAAAATAATTCTTGGTTCTTATGTCTTGAAATGACAACATGGCAGTTTTTTTTTTTTCTACATGAAATTGGAGTATTGAAGTCAAACTTCTTTTGGACTTTAAACCAAACAGCTTTGATATAGAGGGGTATGTGGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCACCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACTTGCATTGGCAAGACTATCATGGATGAAATGGCCTTTAGGTAACAAGAAAACTTACCCTGTACTTTATTTTTTTGTCTAGTTAGCTTTCATTTTTATATCGAGTATGAAAATATCGTGGGAATGAAGATTGAACTTTCGACCTTTTTAGAATGAGGGATTGAACCTTCAACTTTTAGGGGATGAAATGATGGAATGATGGAATGTGTATATTGCAATTGTGGGGTTGGGATTAATCCTTGGACCTCTAGGAAAAAGAATGTATGTCAATTGCGAGAGAGTTAAAGCTTCTATAGACACACACCTCATAACCTTTGCGATTCCTTAGTTGGTTTAGCTATATTATTATCATTCTTTTTCAAGCAGACAATTCTGCCTTCAAATTGGTTAAGACATGACATCAAGGTGAACTTGTGAGGTTTCTAATTTGTAATTGAAATGTTTGACCAGTTGGTTCGTTCCAATTCCTAACATACACAGGCTTTTGAATGAATCTTAATCATATTTTTGGCAGTATAAATGGGGAAAACTTTCATTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGCGTGATTTTATTTCTAAGTCCTTGAAAAACTTGTGATTCCATGTCTTGAACTATTGTAAAAGACCATAACTCAATTTGTAGGAACTGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAATTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACGTCTGGAGTAATACCTATGACACAGAGCTTTGATACAGTAGGTAATGTGTAATCTCTCATTGTCACATTGTCTTTTGTAGTAGATAAGCACAATAGGCTGATTTTTTCTCTGAATTTTAAGTCAACTCTTGATCTCCTTTGTGAAACTGTCTATTTTTTTCAACTAAGGCCCTTTGGTGCTGTCATGCAAAGTTGTTTCCTTTTTTCTTTTTTAACTTTTACTTTTTAATTTTATAGGTAAAAAGGTTTTTTTGACAAACATTTGTGTTTTTTTTTTAAAAAAAAGAACCTAAAGTTTGGAAATACATTACTCCATAATTTCCATTAAAATCATGACATCATTTTGATGATAATGTCTATGTTATTCATGATTTTGCATATAGATGAGCAAAGTGATGTAGATTCATAGCAGAGCCATTGGCGTCTCCTCTTTCCTTCAATACCAACTATATTTCGCTAGCTATCCTGCCATGTGAAAATGGTGTAGATTCCGCTAACACCATCTTCTCGTTTTAGGTTGGTTTGCCAGAGATTCTGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAACCAGAGATTGAGCATTACAAGCCTACGAAGGTGTTCATTGCAGAAGATTGTTTCAAGCTTCTCTCAAACATTACTAGTGAACGATTGACACAAGCTTTTGTTAGCTCAGTGGAGAAGTTATTTGGTGGTAAGTTTAAGCTGACATTCTTGTATTAGTGATTTTGTGTTTTTTAAATTGGGTACCCTTTCATTTAGGAAGAATAAAGAAAGAGATCAAAGGCATACAAAAATTAGTTCACAATATGAATTCCAATCAAGCATAATACGACCTAAAGTGTAATTACAAAATCTTTTTAACACCAAGGCTGGAAGAGAGGTATCAAATCTAACAAGGGACTAAAAACTTCTCCCCAAGAACTTGCAAACCTCTTAACATCAAGCCAATGAATCCATAAAACTGCTCAATAATGGGGTCTAGATTTTTTTTTAAAAATAATGTGTTATGATGTTTGTTTCTGTGCTTCTTAGATAACTAATATACTGGATGGAAGAAAATTTTGAAAACTACTTAGAATTGGTGCATTTGATCCCTGAGTTTTCAAATACACCTTTTTAGTCTCCAAATAGTTTTAAGAGGCCCTTGAAGTATTTTTTTTTTTATTTCTATAATAATTATATGACATTTTGATTTACGAAATAAGTTTAAAAAATAATTTTAGGGAGAAGATGTAGTTTTAAAAAAATCATTTTTCAAACTTAAAAGAACTAAGAAAGAGGCACTTTCTAAACCTCTATTTAAAACTTCATGGACTAAAAAGGTGTATAAAACTTAGGGGACCAAACACATATACTCTAAAGAACTTGAGGACTTAAAAATGTATGTTTGGAAACTCAGAGACTAAACACACGATTCTTCAAAACTCTGGACTAAAAAGGAAAATTTTTCAAAAAGATTACTAATTTGATATTGGAGCTCATCTTTTAGATATACATTTAGGCGATTACTTATTTGACTCTTCAACTGCAGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAAGTTCCAAGCTTGAAGCATTTCATGATTGAAGAAAATGATGGCTATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTTCTTCAGAGGTTTTGATCTTGTGATGAACCACAACTCCAATTCTGTCGAGCATATTGACTCTGATATATAATTGTGTTGAATGAATGAATGATCTTTGCCCATGTTTTTGCTGCCCGCTTGCAGGCATGAATTCAACATCAATCATGGAGAATGGGTTCGGTCCTACCATTCTCATTTGGGTCCAGGAATATCAGAACGAATATTAGAATTCATCAGGGAGGTAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGCTCTTGCTGCTCTTGTTGAAGTATTTCCTTTTTCCCACACACACAATTTACTAAATATTAAACTAATATAATCAGTATCTTAAACATGTGTGCATTTTGATATGTACTCAACTTTCATTGCTTTTTTGTGTAGAAAGAAAAAAAGCTATAACCAATGTAGTTTCCATTTGATATTTCAACATAATTATAGTGTCACTGGTCATTTTATTACTGATTTGATAGTTGCTATTGGATCGTCTACGTCGGAATAACGCAATGCTTCTTTTAGAAAAAAGGTTTACATGTATGATTACATTGTGTCATCTAACAAACATATCAAATTTCTTGGAAATTTATTTCTAGAACTATAATAGGACATTGGTGTTTGTTTCCCGAGAAGATTTATTCTATCTCAAAAATCTATTATTAGACATTTCACTATAGATATAAGCAGTTTGTGAATTTTAAAAAAATCTTGAGTTGCTATTGTTTATCATTTCCTTTCAGGATTTTGGAGTTCTTGCAATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATCTCAGAGCAATACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTCTCTCTCTCTCTTGAAAGTCTTTTCTTTCAAGTACAAGTGAGGTTGGGGGGATTTGAACCATAGACCTCTTGATTCTTAGCACTTGTAGTTATTGGTTGACCTCTCTTACTTGAAAGTTAAAAAGCAACCATTTTGATGTATTTTTTTTCTCACCAACTAATCCGCAGGTTAGCGTACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGTGCAGATGGATTTTTGCTCAATCTTGTTGATAGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAA

mRNA sequence

TAAAGATCAGCTCTGCAATTTCTGGCCATTATTAGGGCGGTTGTGGTGGAGTCTGATTAGGGAGAGCGATCTTAGGCTTTTGCAATGGCTGTGGATGATTATGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCTTCCGATAAACTTCCCTTAAGTGGACTCACTTTCGCTGTTAAAGACATCTTTGATATAGAGGGGTATGTGGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCACCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACTTGCATTGGCAAGACTATCATGGATGAAATGGCCTTTAGTATAAATGGGGAAAACTTTCATTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGAACTGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAATTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACGTCTGGAGTAATACCTATGACACAGAGCTTTGATACAGTAGGTTGGTTTGCCAGAGATTCTGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAACCAGAGATTGAGCATTACAAGCCTACGAAGGTGTTCATTGCAGAAGATTGTTTCAAGCTTCTCTCAAACATTACTAGTGAACGATTGACACAAGCTTTTGTTAGCTCAGTGGAGAAGTTATTTGGTGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAAGTTCCAAGCTTGAAGCATTTCATGATTGAAGAAAATGATGGCTATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTTCTTCAGAGGCATGAATTCAACATCAATCATGGAGAATGGGTTCGGTCCTACCATTCTCATTTGGGTCCAGGAATATCAGAACGAATATTAGAATTCATCAGGGAGGTAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGCTCTTGCTGCTCTTGTTGAAGATTTTGGAGTTCTTGCAATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATCTCAGAGCAATACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTAGCGTACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGTGCAGATGGATTTTTGCTCAATCTTGTTGATAGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAA

Coding sequence (CDS)

ATGGCTGTGGATGATTATGGAGCTTTCATAGAGAAATTTCTCCTGCAGATGAGCTCTCCTTCCGATAAACTTCCCTTAAGTGGACTCACTTTCGCTGTTAAAGACATCTTTGATATAGAGGGGTATGTGGCTGGTTTTGGAAATCCTGAATGGCTAAGGACTCACCCACCTGCCAATCAAACGGCACCAGCTGTGTTGACTATCCTAAGAGGAGGGGCCACTTGCATTGGCAAGACTATCATGGATGAAATGGCCTTTAGTATAAATGGGGAAAACTTTCATTATGGCACACCCCAAAACCCACATGCATCAGATCGGATACCTGGAGGATCTTCCAGTGGTTCTGGTGTTGCTGTTGCTGCAAAGCTTGTGGATTTCTCCTTAGGAACTGATACCGGAGGTAGTGTAAGAGTGCCTGCATCCTACTGTGGAATTCTCGGATTCCGGCCTTCACATGGTGTGGTCTCTACGTCTGGAGTAATACCTATGACACAGAGCTTTGATACAGTAGGTTGGTTTGCCAGAGATTCTGCCATATTAAAGAGAGTAGGTTGGCTGCTGCTGCAAGAACCAGAGATTGAGCATTACAAGCCTACGAAGGTGTTCATTGCAGAAGATTGTTTCAAGCTTCTCTCAAACATTACTAGTGAACGATTGACACAAGCTTTTGTTAGCTCAGTGGAGAAGTTATTTGGTGGCCATCTTATTAAACAAATTAGCCTTGGAAAGTATGTCGAGGATAAAGTTCCAAGCTTGAAGCATTTCATGATTGAAGAAAATGATGGCTATAAGCATAGCATACCATCCTTGGCAGCCCTTGTAAGATCTAAGCGATTTCTTCAGAGGCATGAATTCAACATCAATCATGGAGAATGGGTTCGGTCCTACCATTCTCATTTGGGTCCAGGAATATCAGAACGAATATTAGAATTCATCAGGGAGGTAACAGATGAAAATATTGATCTGAGTCGCTCCATTCAAATCGAACTGCGCGAAGCTCTTGCTGCTCTTGTTGAAGATTTTGGAGTTCTTGCAATTCCTACAGTCCCAGGCCCTCCTCCAAAACTAAACACAGACATCTCAGAGCAATACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCACCATTGCTGCTGTCTCTGGAGTCTGCCAGGTTAGCGTACCTCTAGGCTTGTACAATGGTCTTCCTGTATCAATATCTTTGCTAGCAAAACATGGTGCAGATGGATTTTTGCTCAATCTTGTTGATAGTCTTTACAGCACTCTAAAAGAAGAAGTTGAGGCAATCTACTAA

Protein sequence

MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYSTLKEEVEAIY*
Homology
BLAST of CSPI01G26690 vs. ExPASy Swiss-Prot
Match: Q9FR37 (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.1e-144
Identity = 261/419 (62.29%), Postives = 319/419 (76.13%), Query Frame = 0

Query: 4   DDYGAFIEKFLLQMSSPSDKLP-LSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTA 63
           +D+GAFIEK  +  +S S   P L GLTFA+KDIFD+EG V GFGNP+WLRTH  A  TA
Sbjct: 5   NDFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTA 64

Query: 64  PAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAK 123
           P V ++L  GAT +G TIMDEMA+SINGEN HYGTP+NP A DR+PGGSSSGS VAVAA+
Sbjct: 65  PVVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAAR 124

Query: 124 LVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKR 183
           LVDFS+GTDTGGSVRVPASYCGI GFRPSHG VST G+ PM QSFDTVGWFARD+A LKR
Sbjct: 125 LVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKR 184

Query: 184 VGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGH-LIKQISL 243
           VG +LLQ+  +   +P+++ IA+DCFKL S +  + L Q  V SVEK FGG+ ++K+++L
Sbjct: 185 VGCVLLQQHHLNPIEPSQLIIADDCFKLCS-VPHDLLVQPLVGSVEKSFGGNTVVKKVNL 244

Query: 244 GKYVEDKVPSLKHFMIEEN--DGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           G+Y+   VPSLKHFM  ++     +  IPSL AL  S R LQRHEF INHG W+ S    
Sbjct: 245 GEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPE 304

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTD 363
            GPGISERI E IR  +DE ID  RS++ EL  AL+ L+ + GVL IPTVPGPPP L  +
Sbjct: 305 FGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQAN 364

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSL 419
           ++    FR++AFSLL+IA VSG CQVS+PLGL+  LPVS+SL+AK+G+DGFLL+LVDSL
Sbjct: 365 VAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of CSPI01G26690 vs. ExPASy Swiss-Prot
Match: Q7XTK3 (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 5.0e-134
Identity = 239/421 (56.77%), Postives = 307/421 (72.92%), Query Frame = 0

Query: 5   DYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPA 64
           DYGAF+E+F+L    PS +LPL GLTFA+KDIFDI G V GFGNP+W RTH PA  T+P 
Sbjct: 10  DYGAFMERFVLP-PPPSQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPV 69

Query: 65  VLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLV 124
           VL  L  GAT +G TIMDEMA+SINGEN HYGTP NP A  R+PGGSSSGS VAVAA LV
Sbjct: 70  VLAALAAGATSLGTTIMDEMAYSINGENTHYGTPTNPCAPGRVPGGSSSGSAVAVAANLV 129

Query: 125 DFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKRVG 184
           DFSLGTDTGGSVRVPA+YCGI G RPSHG+VS   VIPM Q FDTVGWF+RD + L RV 
Sbjct: 130 DFSLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLSTLSRVT 189

Query: 185 WLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKY 244
            +LL  P+    +PT+V I  DCF++L ++  +R  Q   +SV K F   ++   +LG +
Sbjct: 190 KVLLPLPDDIVKQPTQVTIPMDCFQILGSL-DDRTYQIINASVAKRFDSQILDNRNLGDF 249

Query: 245 VEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGI 304
           + D VPS+  F+ + ++    S+P+L+ +    R LQR +F  NH EWV +   +LGPG+
Sbjct: 250 ISDNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPNLGPGL 309

Query: 305 SERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTDISEQY 364
            ERILE I    +E+++  ++I+ E + ALAAL++D G+LAIPTVPGPPPK+  + +   
Sbjct: 310 RERILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGMEAAPLE 369

Query: 365 DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYSTLKE 424
           +FRA+AFSLL+IA +SG CQVS+PLG+ NGLPVS+SL+A+HGAD FLLN+V+ LY TL +
Sbjct: 370 NFRARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEELYQTLID 428

Query: 425 E 426
           E
Sbjct: 430 E 428

BLAST of CSPI01G26690 vs. ExPASy Swiss-Prot
Match: Q9MUK5 (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 2.1e-116
Identity = 211/428 (49.30%), Postives = 294/428 (68.69%), Query Frame = 0

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAF+ K  L      + P    PLS L FA+ DIFDIEG+V+ FG+PEW RTH PA+
Sbjct: 35  EDFGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPAS 94

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
            TA AV  ++  GATCIG T++DE+A+ I+GEN H+GTP NP   +R+PGGSSSG+ VAV
Sbjct: 95  STASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAV 154

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           AA  VDFSLG DT G VRVPA +CGILGFRPSHG VS  G+IP++ S DTVGWFA+D  +
Sbjct: 155 AANFVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVGWFAKDPDV 214

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P +    P ++ IA+DCF+ L N+  +R +Q  + + EKLFG  ++K I
Sbjct: 215 LRRVGHILLQAPFVMQRNPRQIIIADDCFQHL-NVPLDRTSQVVIKATEKLFGKQVLKHI 274

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           +   Y+  KV SLK   I++++G   S  SL  L    + LQRHEF   H EW+      
Sbjct: 275 NFEDYISSKVSSLKACSIQKSNGVLKS-SSLKLLANVMQSLQRHEFEHTHSEWMSIVKPD 334

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTD 363
           L P +S ++ E   EV++  I+ S+S++ ELR A+ +L++D GVL IPTV  PPPKL   
Sbjct: 335 LHPAVSAQLHEKF-EVSELEIENSKSVRSELRVAVNSLLKDEGVLVIPTVADPPPKLGGK 394

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLY 423
               +D++++A SLL+IA++SG CQV+VPLG ++  PVS+SL+A+HG D FLL+ + ++Y
Sbjct: 395 EFLSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLDTLKTMY 454

Query: 424 STLKEEVE 428
           + L+E+ +
Sbjct: 455 TVLQEQAD 459

BLAST of CSPI01G26690 vs. ExPASy Swiss-Prot
Match: Q9LVH5 (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 8.3e-113
Identity = 204/425 (48.00%), Postives = 285/425 (67.06%), Query Frame = 0

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAFI+K +L    Q + P    PL+GLTFAV D+FDI GYV GFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
            T+P V T++ GGATC+GKT++DE AFSI+GEN HY +P NP A  RIPGG+ SG+ VAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           A   VDF+LG DT G VRVPA YCG+LGF+ S+G +S +G+IP++ S D+VGWFARD   
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P      P ++ +A+DCF+LL  I  +R+TQ    S EKLFG  L+K  
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLL-KIPVDRITQVVTKSAEKLFGRQLLKHQ 271

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           +L  Y E KVPSLK F   +       + +   L    + LQRHEF  NHG+W+ +    
Sbjct: 272 NLETYFETKVPSLKEFARTKAIA-NTKVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPA 331

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTD 363
           + P I  ++ E   E+T+E  +   +I+ E R A+ +L++D G+L IPT+P  PPKL + 
Sbjct: 332 IDPVILSQVCE-NPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSK 391

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLY 423
                D++ +A SLL+IA++SG CQV+VPLG +   P+S+S + +HG D FLL+ V ++Y
Sbjct: 392 EITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMY 451

Query: 424 STLKE 425
            +L+E
Sbjct: 452 PSLQE 453

BLAST of CSPI01G26690 vs. ExPASy Swiss-Prot
Match: F4KCL7 (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 3.3e-101
Identity = 189/429 (44.06%), Postives = 279/429 (65.03%), Query Frame = 0

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAF+++F L        P+ K  LSGLTF++ D FD++ Y+ GFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
           +TA  V T+L+ GATC+GKTIMDE+ F I GEN HYGTP NP   D +PGG SSGS V+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
            A+LVDFSLG DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA D ++
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L +VG  LL    + H +   +  A+D F+ LS+I  ++  Q    ++E L G    K +
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFE-LSDIPKQKSVQVVRKAIENLSGYKTPKHV 284

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           ++G+YV   VPSL  F  E++   ++S  +L AL      +QRHEF  NH EW ++  S 
Sbjct: 285 NVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSF 344

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTD 363
           LGP  S  ++  ++   +E+I     ++ E+R  + +L+++ G+L IPTV  PPP+LNT 
Sbjct: 345 LGPRFSNDVVTALKS-KNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTK 404

Query: 364 ISEQY-DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSL 423
            ++   +F  + ++L  IA++SG CQV++PLG +   P+S+SLL  +G D FLL+    +
Sbjct: 405 RNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDV 464

Query: 424 YSTLKEEVE 428
           Y++L+++ +
Sbjct: 465 YASLQDQAK 470

BLAST of CSPI01G26690 vs. ExPASy TrEMBL
Match: A0A0A0LZ24 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570120 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 5.9e-247
Identity = 429/430 (99.77%), Postives = 430/430 (100.00%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISERILEFIREVTDENIDLSRSIQIELREALAAL+EDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS
Sbjct: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of CSPI01G26690 vs. ExPASy TrEMBL
Match: A0A1S3BQD5 (amidase 1-like OS=Cucumis melo OX=3656 GN=LOC103492181 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.8e-235
Identity = 409/430 (95.12%), Postives = 420/430 (97.67%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEG+VAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV SY SHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISE+ILEFIRE TDENIDLSRSIQIELRE LAAL+EDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLNLVD LYS
Sbjct: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of CSPI01G26690 vs. ExPASy TrEMBL
Match: A0A5D3CEN2 (Amidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003780 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.2e-233
Identity = 409/437 (93.59%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEG+VAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSL-------GTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSL       GTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLGTIPQFVGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG 240
           ARDSAILKRVGWLLLQEPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGG
Sbjct: 181 ARDSAILKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGG 240

Query: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV 300
           HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV
Sbjct: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWV 300

Query: 301 RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPP 360
            SY SHLGPGISE+ILEFIRE TDENIDLSRSIQIELRE LAAL+EDFGVLAIPTVPGPP
Sbjct: 301 GSYSSHLGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPP 360

Query: 361 PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN 420
           PKLNTDISEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLN
Sbjct: 361 PKLNTDISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLN 420

Query: 421 LVDSLYSTLKEEVEAIY 431
           LVD LYSTLKEEVEAIY
Sbjct: 421 LVDCLYSTLKEEVEAIY 437

BLAST of CSPI01G26690 vs. ExPASy TrEMBL
Match: A0A5A7UBG8 (Amidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00040 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.1e-232
Identity = 408/437 (93.36%), Postives = 418/437 (95.65%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSL-------GTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSL       GTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLGTIPQFVGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG 240
           ARDSAILKRVGWLLL EPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGG
Sbjct: 181 ARDSAILKRVGWLLLHEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGG 240

Query: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV 300
           HLIKQISLGKYVEDKVPSLKHF IEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV
Sbjct: 241 HLIKQISLGKYVEDKVPSLKHFTIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWV 300

Query: 301 RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPP 360
            SY SHLGPGISE+ILEFIRE TDENIDLSRSIQIELRE LAAL+EDFGVLAIPTVPGPP
Sbjct: 301 GSYSSHLGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPP 360

Query: 361 PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN 420
           PKLNTDISEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLN
Sbjct: 361 PKLNTDISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLN 420

Query: 421 LVDSLYSTLKEEVEAIY 431
           LVD LYSTLKEEVEAIY
Sbjct: 421 LVDCLYSTLKEEVEAIY 437

BLAST of CSPI01G26690 vs. ExPASy TrEMBL
Match: A0A6J1I0H5 (amidase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.1e-215
Identity = 373/430 (86.74%), Postives = 401/430 (93.26%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAV D+GAFIEKF+LQ+SSPSD LPL+GLTFAVKDIFD+EGYV GFGNPEWLRTHPPANQ
Sbjct: 1   MAVQDHGAFIEKFILQLSSPSDPLPLAGLTFAVKDIFDMEGYVTGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVL ILRGGATCIGKTIMDEMAFSI+GENFHYGTP+NP+ASDRIPGGSSSGS VAV 
Sbjct: 61  TAPAVLAILRGGATCIGKTIMDEMAFSISGENFHYGTPRNPYASDRIPGGSSSGSAVAVG 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFA DSA+ 
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFAIDSAVF 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVG LLLQEPE+EHYKPT+V IAEDCFK LS+I SERLTQAFV+SV+KLFG HLIKQIS
Sbjct: 181 KRVGRLLLQEPEVEHYKPTQVLIAEDCFK-LSSIPSERLTQAFVNSVKKLFGDHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           +G YVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSK  LQRHEF INHGEWVRSY SHL
Sbjct: 241 IGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTDI 360
           GPG SERI E+IR  TDEN DLS S+QIELR ALAAL+EDFGVL IPTVPGPPPKLNTDI
Sbjct: 301 GPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPPKLNTDI 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SE +DFRAKAFSLL+IA+VSG+CQ ++PLGLYNGLPVSISLLAKHG+DGFLLN+VD+LYS
Sbjct: 361 SELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLYS 420

Query: 421 TLKEEVEAIY 431
           TLK+EVEA Y
Sbjct: 421 TLKQEVEASY 429

BLAST of CSPI01G26690 vs. NCBI nr
Match: XP_004135625.1 (amidase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 862.8 bits (2228), Expect = 1.2e-246
Identity = 429/430 (99.77%), Postives = 430/430 (100.00%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISERILEFIREVTDENIDLSRSIQIELREALAAL+EDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS
Sbjct: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of CSPI01G26690 vs. NCBI nr
Match: KAE8653373.1 (hypothetical protein Csa_007619 [Cucumis sativus])

HSP 1 Score: 855.9 bits (2210), Expect = 1.5e-244
Identity = 429/437 (98.17%), Postives = 430/437 (98.40%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSL-------GTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSL       GTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLGTITQFVGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG 240
           ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG
Sbjct: 181 ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG 240

Query: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV 300
           HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV
Sbjct: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV 300

Query: 301 RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPP 360
           RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAAL+EDFGVLAIPTVPGPP
Sbjct: 301 RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALLEDFGVLAIPTVPGPP 360

Query: 361 PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN 420
           PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN
Sbjct: 361 PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN 420

Query: 421 LVDSLYSTLKEEVEAIY 431
           LVDSLYSTLKEEVEAIY
Sbjct: 421 LVDSLYSTLKEEVEAIY 437

BLAST of CSPI01G26690 vs. NCBI nr
Match: XP_008450659.1 (PREDICTED: amidase 1-like [Cucumis melo])

HSP 1 Score: 824.7 bits (2129), Expect = 3.7e-235
Identity = 409/430 (95.12%), Postives = 420/430 (97.67%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEG+VAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180
           AKLVDFSLGTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL
Sbjct: 121 AKLVDFSLGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAIL 180

Query: 181 KRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQIS 240
           KRVGWLLLQEPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGGHLIKQIS
Sbjct: 181 KRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIKQIS 240

Query: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHL 300
           LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV SY SHL
Sbjct: 241 LGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYSSHL 300

Query: 301 GPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTDI 360
           GPGISE+ILEFIRE TDENIDLSRSIQIELRE LAAL+EDFGVLAIPTVPGPPPKLNTDI
Sbjct: 301 GPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLNTDI 360

Query: 361 SEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLYS 420
           SEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLNLVD LYS
Sbjct: 361 SEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDCLYS 420

Query: 421 TLKEEVEAIY 431
           TLKEEVEAIY
Sbjct: 421 TLKEEVEAIY 430

BLAST of CSPI01G26690 vs. NCBI nr
Match: TYK10231.1 (amidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 817.8 bits (2111), Expect = 4.5e-233
Identity = 409/437 (93.59%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEG+VAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGHVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSL-------GTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSL       GTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLGTIPQFVGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG 240
           ARDSAILKRVGWLLLQEPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGG
Sbjct: 181 ARDSAILKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGG 240

Query: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV 300
           HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV
Sbjct: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWV 300

Query: 301 RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPP 360
            SY SHLGPGISE+ILEFIRE TDENIDLSRSIQIELRE LAAL+EDFGVLAIPTVPGPP
Sbjct: 301 GSYSSHLGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPP 360

Query: 361 PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN 420
           PKLNTDISEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLN
Sbjct: 361 PKLNTDISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLN 420

Query: 421 LVDSLYSTLKEEVEAIY 431
           LVD LYSTLKEEVEAIY
Sbjct: 421 LVDCLYSTLKEEVEAIY 437

BLAST of CSPI01G26690 vs. NCBI nr
Match: KAA0050879.1 (amidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 815.5 bits (2105), Expect = 2.2e-232
Identity = 408/437 (93.36%), Postives = 418/437 (95.65%), Query Frame = 0

Query: 1   MAVDDYGAFIEKFLLQMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60
           MAVDD GAFIEKFLLQMSSPSD+LPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ
Sbjct: 1   MAVDDCGAFIEKFLLQMSSPSDQLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQ 60

Query: 61  TAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVA 120
           TAPAVLTILRGGATCIGKT+MDEMAFSINGENFHYGTPQNP AS RIPGGSSSGSGVAVA
Sbjct: 61  TAPAVLTILRGGATCIGKTMMDEMAFSINGENFHYGTPQNPCASGRIPGGSSSGSGVAVA 120

Query: 121 AKLVDFSL-------GTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWF 180
           AKLVDFSL       GTDTGGSVRVPASYCG+LGFRPSHGVVSTSGVIPMTQSFDTVGWF
Sbjct: 121 AKLVDFSLGTIPQFVGTDTGGSVRVPASYCGVLGFRPSHGVVSTSGVIPMTQSFDTVGWF 180

Query: 181 ARDSAILKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGG 240
           ARDSAILKRVGWLLL EPE+EHYKPTKVFIAEDCFKLLS+ITSERLTQAFVSSVEKLFGG
Sbjct: 181 ARDSAILKRVGWLLLHEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGG 240

Query: 241 HLIKQISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWV 300
           HLIKQISLGKYVEDKVPSLKHF IEENDGYKHSIPSLAALVRSKR LQRHEF INHGEWV
Sbjct: 241 HLIKQISLGKYVEDKVPSLKHFTIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWV 300

Query: 301 RSYHSHLGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPP 360
            SY SHLGPGISE+ILEFIRE TDENIDLSRSIQIELRE LAAL+EDFGVLAIPTVPGPP
Sbjct: 301 GSYSSHLGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPP 360

Query: 361 PKLNTDISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLN 420
           PKLNTDISEQ+DFRAKAFSLLTIAAVSGVCQVS+PLGLYNGLPVSISLLAKHG+DGFLLN
Sbjct: 361 PKLNTDISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLN 420

Query: 421 LVDSLYSTLKEEVEAIY 431
           LVD LYSTLKEEVEAIY
Sbjct: 421 LVDCLYSTLKEEVEAIY 437

BLAST of CSPI01G26690 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 513.1 bits (1320), Expect = 2.2e-145
Identity = 261/419 (62.29%), Postives = 319/419 (76.13%), Query Frame = 0

Query: 4   DDYGAFIEKFLLQMSSPSDKLP-LSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTA 63
           +D+GAFIEK  +  +S S   P L GLTFA+KDIFD+EG V GFGNP+WLRTH  A  TA
Sbjct: 5   NDFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTA 64

Query: 64  PAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAK 123
           P V ++L  GAT +G TIMDEMA+SINGEN HYGTP+NP A DR+PGGSSSGS VAVAA+
Sbjct: 65  PVVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAAR 124

Query: 124 LVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAILKR 183
           LVDFS+GTDTGGSVRVPASYCGI GFRPSHG VST G+ PM QSFDTVGWFARD+A LKR
Sbjct: 125 LVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKR 184

Query: 184 VGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGH-LIKQISL 243
           VG +LLQ+  +   +P+++ IA+DCFKL S +  + L Q  V SVEK FGG+ ++K+++L
Sbjct: 185 VGCVLLQQHHLNPIEPSQLIIADDCFKLCS-VPHDLLVQPLVGSVEKSFGGNTVVKKVNL 244

Query: 244 GKYVEDKVPSLKHFMIEEN--DGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           G+Y+   VPSLKHFM  ++     +  IPSL AL  S R LQRHEF INHG W+ S    
Sbjct: 245 GEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPE 304

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTD 363
            GPGISERI E IR  +DE ID  RS++ EL  AL+ L+ + GVL IPTVPGPPP L  +
Sbjct: 305 FGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQAN 364

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSL 419
           ++    FR++AFSLL+IA VSG CQVS+PLGL+  LPVS+SL+AK+G+DGFLL+LVDSL
Sbjct: 365 VAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of CSPI01G26690 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 408.7 bits (1049), Expect = 5.9e-114
Identity = 204/425 (48.00%), Postives = 285/425 (67.06%), Query Frame = 0

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAFI+K +L    Q + P    PL+GLTFAV D+FDI GYV GFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
            T+P V T++ GGATC+GKT++DE AFSI+GEN HY +P NP A  RIPGG+ SG+ VAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
           A   VDF+LG DT G VRVPA YCG+LGF+ S+G +S +G+IP++ S D+VGWFARD   
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L+RVG +LLQ P      P ++ +A+DCF+LL  I  +R+TQ    S EKLFG  L+K  
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLL-KIPVDRITQVVTKSAEKLFGRQLLKHQ 271

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           +L  Y E KVPSLK F   +       + +   L    + LQRHEF  NHG+W+ +    
Sbjct: 272 NLETYFETKVPSLKEFARTKAIA-NTKVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPA 331

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTD 363
           + P I  ++ E   E+T+E  +   +I+ E R A+ +L++D G+L IPT+P  PPKL + 
Sbjct: 332 IDPVILSQVCE-NPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSK 391

Query: 364 ISEQYDFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSLY 423
                D++ +A SLL+IA++SG CQV+VPLG +   P+S+S + +HG D FLL+ V ++Y
Sbjct: 392 EITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMY 451

Query: 424 STLKE 425
            +L+E
Sbjct: 452 PSLQE 453

BLAST of CSPI01G26690 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 370.2 bits (949), Expect = 2.3e-102
Identity = 189/429 (44.06%), Postives = 279/429 (65.03%), Query Frame = 0

Query: 4   DDYGAFIEKFLL----QMSSPSDKLPLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPAN 63
           +D+GAF+++F L        P+ K  LSGLTF++ D FD++ Y+ GFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  QTAPAVLTILRGGATCIGKTIMDEMAFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAV 123
           +TA  V T+L+ GATC+GKTIMDE+ F I GEN HYGTP NP   D +PGG SSGS V+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 AAKLVDFSLGTDTGGSVRVPASYCGILGFRPSHGVVSTSGVIPMTQSFDTVGWFARDSAI 183
            A+LVDFSLG DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA D ++
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 LKRVGWLLLQEPEIEHYKPTKVFIAEDCFKLLSNITSERLTQAFVSSVEKLFGGHLIKQI 243
           L +VG  LL    + H +   +  A+D F+ LS+I  ++  Q    ++E L G    K +
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFE-LSDIPKQKSVQVVRKAIENLSGYKTPKHV 284

Query: 244 SLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSH 303
           ++G+YV   VPSL  F  E++   ++S  +L AL      +QRHEF  NH EW ++  S 
Sbjct: 285 NVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSF 344

Query: 304 LGPGISERILEFIREVTDENIDLSRSIQIELREALAALVEDFGVLAIPTVPGPPPKLNTD 363
           LGP  S  ++  ++   +E+I     ++ E+R  + +L+++ G+L IPTV  PPP+LNT 
Sbjct: 345 LGPRFSNDVVTALKS-KNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTK 404

Query: 364 ISEQY-DFRAKAFSLLTIAAVSGVCQVSVPLGLYNGLPVSISLLAKHGADGFLLNLVDSL 423
            ++   +F  + ++L  IA++SG CQV++PLG +   P+S+SLL  +G D FLL+    +
Sbjct: 405 RNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDV 464

Query: 424 YSTLKEEVE 428
           Y++L+++ +
Sbjct: 465 YASLQDQAK 470

BLAST of CSPI01G26690 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 104.0 bits (258), Expect = 3.1e-22
Identity = 60/149 (40.27%), Postives = 80/149 (53.69%), Query Frame = 0

Query: 25  PLSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTIMDEM 84
           PL+G+   VKD    +G  +   +       PP + T  AV  I   G   +GKT MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDAT--AVKKIKELGGIVVGKTNMDEF 166

Query: 85  AFSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCG 144
                 E   +    NP    R+PGGSS GS  AVAA+    SLG+DTGGSVR PAS+CG
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCG 226

Query: 145 ILGFRPSHGVVSTSGVIPMTQSFDTVGWF 174
           ++G +P++G VS  G++    S D +G F
Sbjct: 227 VVGLKPTYGRVSRFGLMAYASSLDVIGCF 253

BLAST of CSPI01G26690 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 102.1 bits (253), Expect = 1.2e-21
Identity = 102/407 (25.06%), Postives = 163/407 (40.05%), Query Frame = 0

Query: 26  LSGLTFAVKDIFDIEGYVAGFGNPEWLRTHPPANQTAPAVLTILRGGATCIGKTIMDEMA 85
           L G+   +KD  D   +    G   WL       + +  V  +   GA  +GK  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 86  FSINGENFHYGTPQNPHASDRIPGGSSSGSGVAVAAKLVDFSLGTDTGGSVRVPASYCGI 145
               G N +YGT +NPH   R  GGSSSGS   VAA L   +LGTD GGSVR+P++ CGI
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGI 316

Query: 146 LGFRPSHGVVSTSGVIPMTQSFDTVGWFAR--DSAILKRVGWLLLQEPEIEHYKPTKVFI 205
            G + ++G    +G +    + + +G  A   + A L     L     +  + KP     
Sbjct: 317 TGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSADRYNLKP----- 376

Query: 206 AEDCF-KLLSNITSERLTQAFVSSVEKLFGGHLIKQISLGKYVEDKVPSLKHFMIEENDG 265
           +  CF KLLS+  S  +    +    K F       IS      DK   +   ++  N G
Sbjct: 377 SPPCFPKLLSHNGSNAIGSLRLGKYTKWFNDVSSSDIS------DKCEDILK-LLSNNHG 436

Query: 266 YK---HSIPSLAALVRSKRFLQRHEFNINHGEWVRSYHSHLGPGISERI-------LEFI 325
            K     +P L      +     H  +I     + S   +   G + ++           
Sbjct: 437 CKVVEIVVPEL------EEMRAAHVISIG-SPTLSSLTPYCEAGKNSKLSYDTRTSFAIF 496

Query: 326 REVTDENIDLSRSIQIELREALAALVEDFGVLAIP----TVPGPPPKLNTDISEQYDFRA 385
           R  +  +   ++ ++  L E    + +D  V+  P    T P  PP    +         
Sbjct: 497 RSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVIPPDALKNGETNIQVTT 556

Query: 386 KAFSLLTIAAVSGVCQVSVPLGL-YNGLPVSISLLAKHGADGFLLNL 415
                +  A + G   +SVP+G    GLP+ + ++ +  A+  +L L
Sbjct: 557 DLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGL 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FR373.1e-14462.29Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1[more]
Q7XTK35.0e-13456.77Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1[more]
Q9MUK52.1e-11649.30Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=... [more]
Q9LVH58.3e-11348.00Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP6... [more]
F4KCL73.3e-10144.06Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64... [more]
Match NameE-valueIdentityDescription
A0A0A0LZ245.9e-24799.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570120 PE=3 SV=1[more]
A0A1S3BQD51.8e-23595.12amidase 1-like OS=Cucumis melo OX=3656 GN=LOC103492181 PE=3 SV=1[more]
A0A5D3CEN22.2e-23393.59Amidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003780... [more]
A0A5A7UBG81.1e-23293.36Amidase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00040... [more]
A0A6J1I0H52.1e-21586.74amidase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135625.11.2e-24699.77amidase 1 isoform X1 [Cucumis sativus][more]
KAE8653373.11.5e-24498.17hypothetical protein Csa_007619 [Cucumis sativus][more]
XP_008450659.13.7e-23595.12PREDICTED: amidase 1-like [Cucumis melo][more]
TYK10231.14.5e-23393.59amidase 1-like [Cucumis melo var. makuwa][more]
KAA0050879.12.2e-23293.36amidase 1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G08980.12.2e-14562.29amidase 1 [more]
AT3G17970.15.9e-11448.00translocon at the outer membrane of chloroplasts 64-III [more]
AT5G09420.12.3e-10244.06translocon at the outer membrane of chloroplasts 64-V [more]
AT3G25660.13.1e-2240.27Amidase family protein [more]
AT5G64440.11.2e-2125.06fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 9..424
e-value: 2.3E-89
score: 302.5
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 12..414
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 306..412
e-value: 3.4E-7
score: 29.7
coord: 21..176
e-value: 8.3E-51
score: 173.4
NoneNo IPR availablePANTHERPTHR46310AMIDASE 1coord: 4..427
NoneNo IPR availablePANTHERPTHR46310:SF7AMIDASE 1coord: 4..427
IPR020556Amidase, conserved sitePROSITEPS00571AMIDASEScoord: 109..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G26690.1CSPI01G26690.1mRNA