CSPI01G25660 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G25660
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionagamous-like MADS-box protein AGL80
LocationChr1: 21082546 .. 21083378 (-)
RNA-Seq ExpressionCSPI01G25660
SyntenyCSPI01G25660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAGGAAGAAAGTGAAACTTGCTTACATTGCAAATGACTCTGCAAGAAAGGCAACATACAAGAAGAGGAAGAGAGGGCTTATGAAAAAGGTTAGTGAATTAAGCACTCTTTGTGGGATTGAGGCATGTGCAATCATCTTTAGTCCCTATGATTCCCAACCCGAGCTTTGGCCTTCCCCTATTGGCGTCCAGCGCGTTCTTTCCCAGTTCAAGAAGATGCCCGAGATGGAGCAAAGTAAGAAGATGGTAAGTGTAGTAGTAGTGCATGCTCTTGAAACGTTTATAAAAAAACATATTAATAAGGTGATCATATTGCCATATTATATGTATATAATTAAACGTGTAGCTTCTTAAAGAAAATAATAGAGAGGTATGAATGTTTTAAACAGGTGAACCAGGAGACATTTTTGCGACAAAGGATTGCAAAGGCGAACGAGCAATTGAAGAAAATGAGAAAGGACAACCGGGAGAAAGAGATCACACGGTTGATGTTTCAGAGCCTCACCGCTGCAAAGGGACTACATGGTCTTAACATGCTTGATTTGAACGACCTCGGTTGGCTCATTGATCAAAATCTTAAAGATATTACTATACGCATTGACTCTCTTAAAATTAAACCATCTTCTTCACAACCTCAAGCTCAAGTTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTCCACCGACGCAGCCACAAACTGCGGCATGGCTGATGGAGCTTGTGAGCCCTCAAGATCAAATGGGGTTTGTTGGGGATGATATGCTTTTGCCTTTTGGGGATCAAACTTATAACCATAACAATGCAATGTGGTCTAATGCCTTTTTCCCTTAG

mRNA sequence

ATGACAAGGAAGAAAGTGAAACTTGCTTACATTGCAAATGACTCTGCAAGAAAGGCAACATACAAGAAGAGGAAGAGAGGGCTTATGAAAAAGGTTAGTGAATTAAGCACTCTTTGTGGGATTGAGGCATGTGCAATCATCTTTAGTCCCTATGATTCCCAACCCGAGCTTTGGCCTTCCCCTATTGGCGTCCAGCGCGTTCTTTCCCAGTTCAAGAAGATGCCCGAGATGGAGCAAAGTAAGAAGATGGTGAACCAGGAGACATTTTTGCGACAAAGGATTGCAAAGGCGAACGAGCAATTGAAGAAAATGAGAAAGGACAACCGGGAGAAAGAGATCACACGGTTGATGTTTCAGAGCCTCACCGCTGCAAAGGGACTACATGGTCTTAACATGCTTGATTTGAACGACCTCGGTTGGCTCATTGATCAAAATCTTAAAGATATTACTATACGCATTGACTCTCTTAAAATTAAACCATCTTCTTCACAACCTCAAGCTCAAGTTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTCCACCGACGCAGCCACAAACTGCGGCATGGCTGATGGAGCTTGTGAGCCCTCAAGATCAAATGGGGTTTGTTGGGGATGATATGCTTTTGCCTTTTGGGGATCAAACTTATAACCATAACAATGCAATGTGGTCTAATGCCTTTTTCCCTTAG

Coding sequence (CDS)

ATGACAAGGAAGAAAGTGAAACTTGCTTACATTGCAAATGACTCTGCAAGAAAGGCAACATACAAGAAGAGGAAGAGAGGGCTTATGAAAAAGGTTAGTGAATTAAGCACTCTTTGTGGGATTGAGGCATGTGCAATCATCTTTAGTCCCTATGATTCCCAACCCGAGCTTTGGCCTTCCCCTATTGGCGTCCAGCGCGTTCTTTCCCAGTTCAAGAAGATGCCCGAGATGGAGCAAAGTAAGAAGATGGTGAACCAGGAGACATTTTTGCGACAAAGGATTGCAAAGGCGAACGAGCAATTGAAGAAAATGAGAAAGGACAACCGGGAGAAAGAGATCACACGGTTGATGTTTCAGAGCCTCACCGCTGCAAAGGGACTACATGGTCTTAACATGCTTGATTTGAACGACCTCGGTTGGCTCATTGATCAAAATCTTAAAGATATTACTATACGCATTGACTCTCTTAAAATTAAACCATCTTCTTCACAACCTCAAGCTCAAGTTCAGGCTCAGGCTCAGGCTCAGGCTCAGGCTCCACCGACGCAGCCACAAACTGCGGCATGGCTGATGGAGCTTGTGAGCCCTCAAGATCAAATGGGGTTTGTTGGGGATGATATGCTTTTGCCTTTTGGGGATCAAACTTATAACCATAACAATGCAATGTGGTCTAATGCCTTTTTCCCTTAG

Protein sequence

MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPSPIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQSLTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAPPTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP*
Homology
[Close]
BLAST of CSPI01G25660 vs. ExPASy Swiss-Prot
Match: Q9FJK3 (Agamous-like MADS-box protein AGL80 OS=Arabidopsis thaliana OX=3702 GN=AGL80 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 9.1e-50
Identity = 112/194 (57.73%), Postives = 142/194 (73.20%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYI+NDS+RKAT+KKRK+GLMKKV ELSTLCGI ACAII+SPYD+ PE+WPS
Sbjct: 1   MTRKKVKLAYISNDSSRKATFKKRKKGLMKKVHELSTLCGITACAIIYSPYDTNPEVWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
             GVQRV+S+F+ +PEM+Q KKMV+QE FL+QRIAKA E L++ RKD+RE E+T +MFQ 
Sbjct: 61  NSGVQRVVSEFRTLPEMDQHKKMVDQEGFLKQRIAKATETLRRQRKDSRELEMTEVMFQC 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK-----IKPSSSQPQAQVQAQAQA 180
           L     +  LN++DLNDLG++I+Q LKD+  RI+ L+     I  SSS   A  +     
Sbjct: 121 LIGNMEMFHLNIVDLNDLGYMIEQYLKDVNRRIEILRNSGTEIGESSSVAVAASEGNIPM 180

Query: 181 QAQAPPTQPQTAAW 190
                 T P T  +
Sbjct: 181 PNLVATTAPTTTIY 194

[Close]
BLAST of CSPI01G25660 vs. ExPASy Swiss-Prot
Match: O80805 (MADS-box transcription factor PHERES 1 OS=Arabidopsis thaliana OX=3702 GN=PHE1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.2e-32
Identity = 77/161 (47.83%), Postives = 111/161 (68.94%), Query Frame = 0 

Query: 3   RKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPSPI 62
           R K+KL++I NDS RK T+ KRK+G++KK +EL TLCG++ACA+I SPY+S  E WPS  
Sbjct: 2   RGKMKLSFIENDSVRKTTFTKRKKGMLKKFNELVTLCGVDACAVIRSPYNSIQEPWPSRE 61

Query: 63  GVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQSLT 122
           GV+ V+S+F +   ++++KKMV+QETFLRQRIAK  E+L+K+R +NR  +I  LMF  L 
Sbjct: 62  GVEEVMSKFMEFSVLDRTKKMVDQETFLRQRIAKETERLQKLRDENRNSQIRDLMFGCLK 121

Query: 123 AAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSS 164
               +  L+  DL DL   +++ L  +  R++ LK    SS
Sbjct: 122 GEVDVSHLHGRDLLDLNVFLNKYLNGVIRRVEILKENGESS 162

[Close]
BLAST of CSPI01G25660 vs. ExPASy Swiss-Prot
Match: Q7XJK8 (MADS-box transcription factor PHERES 2 OS=Arabidopsis thaliana OX=3702 GN=PHE2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.8e-32
Identity = 79/174 (45.40%), Postives = 118/174 (67.82%), Query Frame = 0 

Query: 3   RKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPSPI 62
           ++K+KL+ I N  +RK T+ KRK+G+ KK++EL TLCG+EACA+++SP++S PE WPS  
Sbjct: 2   KRKMKLSLIENSVSRKTTFTKRKKGMTKKLTELVTLCGVEACAVVYSPFNSIPEAWPSRE 61

Query: 63  GVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQSLT 122
           GV+ V+S+F ++  ++++KKMV+QETF+ QRIAK  EQL+K+R +N   +I  LMF  L 
Sbjct: 62  GVEDVVSKFMELSVLDRTKKMVDQETFISQRIAKEKEQLQKLRDENHNSQIRELMFGCLK 121

Query: 123 AAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSL-KIKPSSSQPQAQVQAQAQA 176
               ++ L+  DL DL   ID+ L  +T RI+ L +   SSS     + A A A
Sbjct: 122 GETNVYNLDGRDLQDLSLYIDKYLNGLTRRIEILIENGESSSSLPLPIVANAAA 175

[Close]
BLAST of CSPI01G25660 vs. ExPASy Swiss-Prot
Match: Q7XJK6 (Agamous-like MADS-box protein AGL36 OS=Arabidopsis thaliana OX=3702 GN=AGL36 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.1e-30
Identity = 71/154 (46.10%), Postives = 104/154 (67.53%), Query Frame = 0 

Query: 4   KKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPSPIG 63
           KKVKL+ IAN+ +RK ++ KRK G+ KK+ ELSTLCG++ACA+I+SP+   PE WPS  G
Sbjct: 2   KKVKLSLIANERSRKTSFIKRKDGIFKKLHELSTLCGVQACALIYSPFIPVPESWPSREG 61

Query: 64  VQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQSLTA 123
            ++V S+F +MP   ++KKM++QET+L +RI KA EQLK +  +NRE ++ R MF  +  
Sbjct: 62  AKKVASRFLEMPPTARTKKMMDQETYLMERITKAKEQLKNLAAENRELQVRRFMFDCVEG 121

Query: 124 AKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK 158
               +  +  DL DL   I+  L  +  RI+S+K
Sbjct: 122 KMSQYHYDAKDLQDLQSCINLYLDQLNGRIESIK 155

[Close]
BLAST of CSPI01G25660 vs. ExPASy Swiss-Prot
Match: Q7XJK5 (Agamous-like MADS-box protein AGL90 OS=Arabidopsis thaliana OX=3702 GN=AGL90 PE=1 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 1.4e-29
Identity = 69/154 (44.81%), Postives = 103/154 (66.88%), Query Frame = 0 

Query: 4   KKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPSPIG 63
           KKVKL+ IAN+ +RK ++ KRK G+ KK+ ELSTLCG++ACA+I+SP+   PE WPS  G
Sbjct: 2   KKVKLSLIANERSRKTSFMKRKNGIFKKLHELSTLCGVQACALIYSPFIPVPESWPSREG 61

Query: 64  VQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQSLTA 123
            ++V S+F +MP   +++KM++QET L +RI KA EQLK +  +NRE ++ R MF  +  
Sbjct: 62  AKKVASKFLEMPRTARTRKMMDQETHLMERITKAKEQLKNLAAENRELQVRRFMFDCVEG 121

Query: 124 AKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK 158
               +  +  DL DL   ++  L  +  RI+S+K
Sbjct: 122 KMSQYRYDAKDLQDLLSCMNLYLDQLNGRIESIK 155

[Close]
BLAST of CSPI01G25660 vs. ExPASy TrEMBL
Match: A0A0A0LVS3 (MADS-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G560740 PE=4 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.8e-117
Identity = 225/229 (98.25%), Postives = 225/229 (98.25%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS
Sbjct: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQV    QAQAQAP
Sbjct: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQV----QAQAQAP 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
           PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP
Sbjct: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 225

[Close]
BLAST of CSPI01G25660 vs. ExPASy TrEMBL
Match: A0A5A7U988 (Agamous-like MADS-box protein AGL80 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004670 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 8.7e-112
Identity = 218/229 (95.20%), Postives = 218/229 (95.20%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS
Sbjct: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK KPS  QPQAQ QAQA      P
Sbjct: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK-KPSLPQPQAQAQAQA-----PP 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
            TQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP
Sbjct: 181 TTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 223

[Close]
BLAST of CSPI01G25660 vs. ExPASy TrEMBL
Match: A0A1S3BPF2 (agamous-like MADS-box protein AGL80 OS=Cucumis melo OX=3656 GN=LOC103492094 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 8.7e-112
Identity = 218/229 (95.20%), Postives = 218/229 (95.20%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS
Sbjct: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK KPS  QPQAQ QAQA      P
Sbjct: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK-KPSLPQPQAQAQAQA-----PP 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
            TQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP
Sbjct: 181 TTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 223

[Close]
BLAST of CSPI01G25660 vs. ExPASy TrEMBL
Match: A0A6J1HW01 (agamous-like MADS-box protein AGL80 OS=Cucurbita maxima OX=3661 GN=LOC111467976 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.3e-103
Identity = 200/229 (87.34%), Postives = 206/229 (89.96%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYI NDSARKAT+KKRKRGLMKKVSELSTLCGIEACAI+FSPYD+QPELWPS
Sbjct: 1   MTRKKVKLAYIVNDSARKATFKKRKRGLMKKVSELSTLCGIEACAIVFSPYDAQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKAN+QLKKMRKDNREKE+TRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANDQLKKMRKDNREKEMTRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLH LNMLDLNDL WLIDQNLKDIT RIDSLK  P  SQP      Q       P
Sbjct: 121 LTAAKGLHALNMLDLNDLTWLIDQNLKDITKRIDSLKKPPPRSQPHPHPHPQ-------P 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
           P  PQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTY+HNNAMWSNAFFP
Sbjct: 181 PPPPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYSHNNAMWSNAFFP 222

[Close]
BLAST of CSPI01G25660 vs. ExPASy TrEMBL
Match: A0A6J1HBW4 (agamous-like MADS-box protein AGL80 OS=Cucurbita moschata OX=3662 GN=LOC111462033 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.1e-102
Identity = 199/229 (86.90%), Postives = 205/229 (89.52%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYI NDSARKAT+KKRKRGLMKKVSELSTLCGIEACAI+FSPYD+QPELWPS
Sbjct: 1   MTRKKVKLAYIVNDSARKATFKKRKRGLMKKVSELSTLCGIEACAIVFSPYDAQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKAN+QLKKMRKDNREKE+TRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANDQLKKMRKDNREKEMTRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLH LNMLDLNDL WLIDQNLKDIT RIDSLK  P  SQP      Q       P
Sbjct: 121 LTAAKGLHALNMLDLNDLTWLIDQNLKDITKRIDSLKKPPPHSQPHPHPHPQ-------P 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
           P  PQTAAWLMELVSPQDQMGFV DDMLLPFGDQTY+HNNAMWSNAFFP
Sbjct: 181 PPPPQTAAWLMELVSPQDQMGFVADDMLLPFGDQTYSHNNAMWSNAFFP 222

[Close]
BLAST of CSPI01G25660 vs. NCBI nr
Match: XP_004135760.1 (agamous-like MADS-box protein AGL80 [Cucumis sativus] >KGN65990.1 hypothetical protein Csa_006882 [Cucumis sativus])

HSP 1 Score: 431.8 bits (1109), Expect = 3.7e-117
Identity = 225/229 (98.25%), Postives = 225/229 (98.25%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS
Sbjct: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQV    QAQAQAP
Sbjct: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQV----QAQAQAP 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
           PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP
Sbjct: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 225

[Close]
BLAST of CSPI01G25660 vs. NCBI nr
Match: XP_008450513.1 (PREDICTED: agamous-like MADS-box protein AGL80 [Cucumis melo] >KAA0050967.1 agamous-like MADS-box protein AGL80 [Cucumis melo var. makuwa] >TYK10313.1 agamous-like MADS-box protein AGL80 [Cucumis melo var. makuwa])

HSP 1 Score: 412.9 bits (1060), Expect = 1.8e-111
Identity = 218/229 (95.20%), Postives = 218/229 (95.20%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS
Sbjct: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK KPS  QPQAQ QAQA      P
Sbjct: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK-KPSLPQPQAQAQAQA-----PP 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
            TQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP
Sbjct: 181 TTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 223

[Close]
BLAST of CSPI01G25660 vs. NCBI nr
Match: XP_022968841.1 (agamous-like MADS-box protein AGL80 [Cucurbita maxima])

HSP 1 Score: 384.0 bits (985), Expect = 8.9e-103
Identity = 200/229 (87.34%), Postives = 206/229 (89.96%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYI NDSARKAT+KKRKRGLMKKVSELSTLCGIEACAI+FSPYD+QPELWPS
Sbjct: 1   MTRKKVKLAYIVNDSARKATFKKRKRGLMKKVSELSTLCGIEACAIVFSPYDAQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKAN+QLKKMRKDNREKE+TRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANDQLKKMRKDNREKEMTRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLH LNMLDLNDL WLIDQNLKDIT RIDSLK  P  SQP      Q       P
Sbjct: 121 LTAAKGLHALNMLDLNDLTWLIDQNLKDITKRIDSLKKPPPRSQPHPHPHPQ-------P 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
           P  PQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTY+HNNAMWSNAFFP
Sbjct: 181 PPPPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYSHNNAMWSNAFFP 222

[Close]
BLAST of CSPI01G25660 vs. NCBI nr
Match: XP_023538956.1 (agamous-like MADS-box protein AGL80 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 384.0 bits (985), Expect = 8.9e-103
Identity = 200/229 (87.34%), Postives = 206/229 (89.96%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYI NDSARKAT+KKRKRGLMKKVSELSTLCGIEACAI+FSPYD+QPELWPS
Sbjct: 1   MTRKKVKLAYIVNDSARKATFKKRKRGLMKKVSELSTLCGIEACAIVFSPYDAQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKAN+QLKKMRKDNREKE+TRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANDQLKKMRKDNREKEMTRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLH LNMLDLNDL WLIDQNLKDIT RIDSLK  P  SQP        Q     P
Sbjct: 121 LTAAKGLHALNMLDLNDLTWLIDQNLKDITKRIDSLKKPPPHSQPHPHPHPHPQ-----P 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
           P  PQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTY+HNNAMWSNAFFP
Sbjct: 181 PPPPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYSHNNAMWSNAFFP 224

[Close]
BLAST of CSPI01G25660 vs. NCBI nr
Match: XP_022961458.1 (agamous-like MADS-box protein AGL80 [Cucurbita moschata] >KAG6590517.1 Agamous-like MADS-box protein AGL80, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024050.1 Agamous-like MADS-box protein AGL80, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 381.7 bits (979), Expect = 4.4e-102
Identity = 199/229 (86.90%), Postives = 205/229 (89.52%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYI NDSARKAT+KKRKRGLMKKVSELSTLCGIEACAI+FSPYD+QPELWPS
Sbjct: 1   MTRKKVKLAYIVNDSARKATFKKRKRGLMKKVSELSTLCGIEACAIVFSPYDAQPELWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
           PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKAN+QLKKMRKDNREKE+TRLMFQS
Sbjct: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANDQLKKMRKDNREKEMTRLMFQS 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSSQPQAQVQAQAQAQAQAP 180
           LTAAKGLH LNMLDLNDL WLIDQNLKDIT RIDSLK  P  SQP      Q       P
Sbjct: 121 LTAAKGLHALNMLDLNDLTWLIDQNLKDITKRIDSLKKPPPHSQPHPHPHPQ-------P 180

Query: 181 PTQPQTAAWLMELVSPQDQMGFVGDDMLLPFGDQTYNHNNAMWSNAFFP 230
           P  PQTAAWLMELVSPQDQMGFV DDMLLPFGDQTY+HNNAMWSNAFFP
Sbjct: 181 PPPPQTAAWLMELVSPQDQMGFVADDMLLPFGDQTYSHNNAMWSNAFFP 222

[Close]
BLAST of CSPI01G25660 vs. TAIR 10
Match: AT5G48670.1 (AGAMOUS-like 80 )

HSP 1 Score: 198.4 bits (503), Expect = 6.4e-51
Identity = 112/194 (57.73%), Postives = 142/194 (73.20%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKKVKLAYI+NDS+RKAT+KKRK+GLMKKV ELSTLCGI ACAII+SPYD+ PE+WPS
Sbjct: 1   MTRKKVKLAYISNDSSRKATFKKRKKGLMKKVHELSTLCGITACAIIYSPYDTNPEVWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
             GVQRV+S+F+ +PEM+Q KKMV+QE FL+QRIAKA E L++ RKD+RE E+T +MFQ 
Sbjct: 61  NSGVQRVVSEFRTLPEMDQHKKMVDQEGFLKQRIAKATETLRRQRKDSRELEMTEVMFQC 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK-----IKPSSSQPQAQVQAQAQA 180
           L     +  LN++DLNDLG++I+Q LKD+  RI+ L+     I  SSS   A  +     
Sbjct: 121 LIGNMEMFHLNIVDLNDLGYMIEQYLKDVNRRIEILRNSGTEIGESSSVAVAASEGNIPM 180

Query: 181 QAQAPPTQPQTAAW 190
                 T P T  +
Sbjct: 181 PNLVATTAPTTTIY 194

[Close]
BLAST of CSPI01G25660 vs. TAIR 10
Match: AT5G26630.1 (MADS-box transcription factor family protein )

HSP 1 Score: 156.4 bits (394), Expect = 2.8e-38
Identity = 81/165 (49.09%), Postives = 118/165 (71.52%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTR+KVK+ +I N++ARK+T+KKRK+GL+KK  EL  LCG+   A++ SPY+  PE+WPS
Sbjct: 1   MTRQKVKMTFIENETARKSTFKKRKKGLLKKAQELGILCGVPIFAVVNSPYELNPEVWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
                +V+SQ+K M  M+++KKMVNQETFL+QRI KA E  KK+RK+N+E E+  +MF  
Sbjct: 61  REAANQVVSQWKTMSVMDKTKKMVNQETFLQQRITKATESWKKLRKENKELEMKNIMFDC 120

Query: 121 LTAAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLK--IKPSSS 164
           L+    +  +   +L D G++I+Q LKD+  RI+ LK   +PSS+
Sbjct: 121 LSGKTLVSSIEKTELRDFGYVIEQQLKDVNRRIEILKRNNEPSSA 165

[Close]
BLAST of CSPI01G25660 vs. TAIR 10
Match: AT1G65330.1 (MADS-box transcription factor family protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.6e-33
Identity = 77/161 (47.83%), Postives = 111/161 (68.94%), Query Frame = 0 

Query: 3   RKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPSPI 62
           R K+KL++I NDS RK T+ KRK+G++KK +EL TLCG++ACA+I SPY+S  E WPS  
Sbjct: 2   RGKMKLSFIENDSVRKTTFTKRKKGMLKKFNELVTLCGVDACAVIRSPYNSIQEPWPSRE 61

Query: 63  GVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQSLT 122
           GV+ V+S+F +   ++++KKMV+QETFLRQRIAK  E+L+K+R +NR  +I  LMF  L 
Sbjct: 62  GVEEVMSKFMEFSVLDRTKKMVDQETFLRQRIAKETERLQKLRDENRNSQIRDLMFGCLK 121

Query: 123 AAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSLKIKPSSS 164
               +  L+  DL DL   +++ L  +  R++ LK    SS
Sbjct: 122 GEVDVSHLHGRDLLDLNVFLNKYLNGVIRRVEILKENGESS 162

[Close]
BLAST of CSPI01G25660 vs. TAIR 10
Match: AT1G65300.1 (AGAMOUS-like 38 )

HSP 1 Score: 139.8 bits (351), Expect = 2.7e-33
Identity = 79/174 (45.40%), Postives = 118/174 (67.82%), Query Frame = 0 

Query: 3   RKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPSPI 62
           ++K+KL+ I N  +RK T+ KRK+G+ KK++EL TLCG+EACA+++SP++S PE WPS  
Sbjct: 2   KRKMKLSLIENSVSRKTTFTKRKKGMTKKLTELVTLCGVEACAVVYSPFNSIPEAWPSRE 61

Query: 63  GVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQSLT 122
           GV+ V+S+F ++  ++++KKMV+QETF+ QRIAK  EQL+K+R +N   +I  LMF  L 
Sbjct: 62  GVEDVVSKFMELSVLDRTKKMVDQETFISQRIAKEKEQLQKLRDENHNSQIRELMFGCLK 121

Query: 123 AAKGLHGLNMLDLNDLGWLIDQNLKDITIRIDSL-KIKPSSSQPQAQVQAQAQA 176
               ++ L+  DL DL   ID+ L  +T RI+ L +   SSS     + A A A
Sbjct: 122 GETNVYNLDGRDLQDLSLYIDKYLNGLTRRIEILIENGESSSSLPLPIVANAAA 175

[Close]
BLAST of CSPI01G25660 vs. TAIR 10
Match: AT3G05860.1 (MADS-box transcription factor family protein )

HSP 1 Score: 138.3 bits (347), Expect = 7.9e-33
Identity = 78/151 (51.66%), Postives = 105/151 (69.54%), Query Frame = 0 

Query: 1   MTRKKVKLAYIANDSARKATYKKRKRGLMKKVSELSTLCGIEACAIIFSPYDSQPELWPS 60
           MTRKK+ L+YI N+S RKAT+ KRK+GL+KK+ ELS LCGIEACA+I+SP++S PE+WPS
Sbjct: 1   MTRKKLNLSYITNESMRKATFNKRKKGLVKKIHELSVLCGIEACAVIYSPFNSNPEVWPS 60

Query: 61  PIGVQRVLSQFKKMPEMEQSKKMVNQETFLRQRIAKANEQLKKMRKDNREKEITRLMFQS 120
              V+ V+  F+ + ++EQ KKMV+ E F+RQ I+K  E   K   DN E+ +   MFQ 
Sbjct: 61  NSEVKNVMENFEMLTKLEQEKKMVSHEGFIRQNISKTMESNNKKMIDNAERTMKEAMFQ- 120

Query: 121 LTAAKGLHGLNMLDLN--DLGWLIDQNLKDI 150
           L + KG   LN+ D N  DL   IDQ LK++
Sbjct: 121 LLSGKG-EKLNLTDRNREDLCKYIDQYLKEL 149

The following BLAST results are available for this feature:
BLAST of CSPI01G25660 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FJK39.1e-5057.73Agamous-like MADS-box protein AGL80 OS=Arabidopsis thaliana OX=3702 GN=AGL80 PE=... [more]
O808052.2e-3247.83MADS-box transcription factor PHERES 1 OS=Arabidopsis thaliana OX=3702 GN=PHE1 P... [more]
Q7XJK83.8e-3245.40MADS-box transcription factor PHERES 2 OS=Arabidopsis thaliana OX=3702 GN=PHE2 P... [more]
Q7XJK62.1e-3046.10Agamous-like MADS-box protein AGL36 OS=Arabidopsis thaliana OX=3702 GN=AGL36 PE=... [more]
Q7XJK51.4e-2944.81Agamous-like MADS-box protein AGL90 OS=Arabidopsis thaliana OX=3702 GN=AGL90 PE=... [more]
BLAST of CSPI01G25660 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LVS31.8e-11798.25MADS-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G560740 PE... [more]
A0A5A7U9888.7e-11295.20Agamous-like MADS-box protein AGL80 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3BPF28.7e-11295.20agamous-like MADS-box protein AGL80 OS=Cucumis melo OX=3656 GN=LOC103492094 PE=4... [more]
A0A6J1HW014.3e-10387.34agamous-like MADS-box protein AGL80 OS=Cucurbita maxima OX=3661 GN=LOC111467976 ... [more]
A0A6J1HBW42.1e-10286.90agamous-like MADS-box protein AGL80 OS=Cucurbita moschata OX=3662 GN=LOC11146203... [more]
BLAST of CSPI01G25660 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004135760.13.7e-11798.25agamous-like MADS-box protein AGL80 [Cucumis sativus] >KGN65990.1 hypothetical p... [more]
XP_008450513.11.8e-11195.20PREDICTED: agamous-like MADS-box protein AGL80 [Cucumis melo] >KAA0050967.1 agam... [more]
XP_022968841.18.9e-10387.34agamous-like MADS-box protein AGL80 [Cucurbita maxima][more]
XP_023538956.18.9e-10387.34agamous-like MADS-box protein AGL80 [Cucurbita pepo subsp. pepo][more]
XP_022961458.14.4e-10286.90agamous-like MADS-box protein AGL80 [Cucurbita moschata] >KAG6590517.1 Agamous-l... [more]
BLAST of CSPI01G25660 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G48670.16.4e-5157.73AGAMOUS-like 80 [more]
AT5G26630.12.8e-3849.09MADS-box transcription factor family protein [more]
AT1G65330.11.6e-3347.83MADS-box transcription factor family protein [more]
AT1G65300.12.7e-3345.40AGAMOUS-like 38 [more]
AT3G05860.17.9e-3351.66MADS-box transcription factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 87..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 164..184
NoneNo IPR availablePANTHERPTHR48019:SF17AGAMOUS-LIKE MADS-BOX PROTEIN AGL80coord: 1..188
NoneNo IPR availablePANTHERPTHR48019SERUM RESPONSE FACTOR HOMOLOGcoord: 1..188
IPR002100Transcription factor, MADS-boxPRINTSPR00404MADSDOMAINcoord: 23..38
score: 62.47
coord: 38..59
score: 29.53
coord: 3..23
score: 36.0
IPR002100Transcription factor, MADS-boxSMARTSM00432madsneu2coord: 1..60
e-value: 4.6E-25
score: 99.3
IPR002100Transcription factor, MADS-boxPFAMPF00319SRF-TFcoord: 11..51
e-value: 5.1E-18
score: 64.3
IPR002100Transcription factor, MADS-boxPROSITEPS50066MADS_BOX_2coord: 1..49
score: 17.282614
IPR036879Transcription factor, MADS-box superfamilyGENE3D3.40.1810.10coord: 13..97
e-value: 1.2E-19
score: 72.2
IPR036879Transcription factor, MADS-box superfamilySUPERFAMILY55455SRF-likecoord: 2..95
IPR033897MADS SRF-likeCDDcd00266MADS_SRF_likecoord: 2..85
e-value: 4.6704E-35
score: 117.746

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G25660.1CSPI01G25660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045944 positive regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0000987 cis-regulatory region sequence-specific DNA binding
molecular_function GO:0003677 DNA binding