CSPI01G25280 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G25280
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	primase homolog protein isoform X1
Location	Chr1: 20745682 .. 20751671 (-)
RNA-Seq Expression	CSPI01G25280
Synteny	CSPI01G25280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAACTTTGTGTGTGTATGCGAAGCAGATAAGGCTGCTTCCACTCTGCTCTTCCATTTCTCTCCAAATGCCAATAAACTCAACTTTCACTTTCAATTCTAATTTCAACTTCCAATTCAGATTCATTAATCATCGCTCTTTCCCTTTCTCTCATTCTCTAAACAATTTCTCCATTTCCTCTTCCCCTTCCCGATTCCTTCGGTTCTGCACTACCAGACTATCTTCCGCCGTTTCTCATGCTCCCCCCTCCCCGCCCCCAGGTACTCGTTTTTTTTCTTGAGTTTAGAAAGTGAAACCCCACCCTTTTGTTCCTTTTCGGATTTTGAGGCTAATGTTTTGGGCTTCCATAATTAAAGTGAAGCATCTTATGGAGAGGATGGATCTTGTTGGAATAGTCTGTGATGAACATTGTATCCCTGGAAAATACTATTGCCTCTTCTGTCCCAAGGTTATCTGCTTCTCTTGTTTCTACATTTTGTGGTTTGAATTAATGCATGAAAATCCAAACCCATCTTTTCATTTCAATTGTCTTTTTCTTTTTTGTTAGATTATAGATCATAGACAAGCTCCTTTTTTCAATTTTTTTTTTATGTTCAACACTAAAAATGCATTTTTAAAATTTTGAGATAAAAATAAAACAAAGTTAGCCTAATTTATATATTTCCAGAAGCTTTTAAGTGATTGAAAGTTTAGAAGTTTTATCTATTTCACTTATCAACTTTAAAAATTTTATATTGTGTTCTTAACTTCAAGTTTTTTGTGTCTAGAATTTTTCTTCATTCTACTGGTAACTTCATAAGTTTAGGAGTTATCTCTCCATATGCATGATCTAGCCCGTATGCCAAGTAAGTACTAAATTGTTAAATGCTAATGACATGACATGGACTCATTGGATATAAAGGACCTAAGGAAAGTTGTAGGACTAAATAGAACACAATTTTAAAAGATAAAGACTTGTTGAAACCTCTTATAGGAGCCAAAATAAACATAACTGTTGAAGATAGGAGACCAAACTTGTAATACAACTTTCTTTTATTATTATCTCTATAATTAGATATGTTCACGGTTGACAGTGCAAGGGTGGTCGTTTAATGGAGAGGAGCTTATCTCTTCATGTGATTCCAACTGGGTAAGTAAATTACCATACTTGTTATTATTTTTTCATTTTCTTCATTTCTTATGGAGATTGATTGGAGAGTAAATTGTTGTTAATTACATCCTGTCATAATTTGGAACATAGAGATTTTGCAATGTGGAGGTGTTTCCAGTTTGAGTGTGGTTGGGCAGGGCAGGTAACGTTATGAAAACAATAATGTGTATTTGTAATATTTCTCTTCAATCTCATATCTTGTAATTAAACGTTGAGAATTTTGCTCAGCAAAATGATTGTATGGTTTAGATATTTGCAGATGGCAAATTGGCATTTAATGGATTCGGCAAAATTACAAAAATGGTTGGACGGAGTTCCAAGGAAAGTTTGGTTTTGGAGCCTCTTTGTGATGAGGTACTTCAAATTTCTTTGCATTTCGATGAATAAGTCGTGTTCTTGCTTAACACTTCTACGATTCTTGGATAAGAACTCTAGAAGTTTAGTCCCTTATTCACTTTCACATTTCTTTGTCAGTTTCAGTTTCGAATATCTTTTGTAAATATTCTATAGATGTTCTTCCGCATGGTTAGATCTCCTCAGGTGATGTTTTCGGGAGCTGAGTTTTTTTTAATATGTCCATGTATTCTTTCATTTTCTCTCTTGAGTGAAAGTTGTTGCTTTCATAAAAAAAAATGAACAAGCCATACTCTTACACTGATATGTCGTACCGATTCAATCTTTTGACACAAACATAAGTACTTATTGGTTCAAACAAGAAATTGAGTTAGCTCTTGATTTTGGTTGCTATTGAATTAAAAAATACTTAGAACGTAATTCAAGAGTGTTTTGATCTTTCTTTTTCAGCTAATCTCTTATTTCAGCATGAGAATGATATCCCAAGAAACCTTAGAACGGAATGTTGTTATGCAATTAGCTGGTCGTCAGGTACTTGAAATAATGATTTTGTGTACATGTCTTATAATTGAAATTTTAAACTTGGTTTCTTGTAAATACTTTGCTGCCTAATTTCGTTATAAGTATTGACTTGATATTCTTCGATCTACATGTGGTGGTATTTTCAATGAATCATTTCGGGAGAGACAATTTTTGTTCAAAGTATGTTGCATTCTTCTGTTTATATAATTAGGTGTTTGGTTGTTTTAAAACCTAAAATTTGCATTATATTTCTCTCCTCAATGAAGAGAGGATCTTAGCTCTAAATTGTGGATGGATATCCTAATTACCTCAAGGGCGAATGGTTTCTCTATTAAGGCTCATCATTATAGAATAGGCATTCAATTGGTGTTTAAATTAATGATGATTATTTTCTCTTTTTCCTTTGTATGTTGAACTTCTAACAAGTTTAAAGGCCAATTGCAGGTTACTATTGCATTTACTTATCGACAAAATGGACAGCTTGTTGGTTGCAAGTACCGAACCATGGACAAGAGATTTTGGCAGGTACGAAGTCCTTTTGCAACCATAGCAAATATAGCAATTAGGTCCAATGTTAACGCATTTTTAAATATAAGATAAAGCAAAAGAATTTGCAAAATTTACATTTAGATCAAACTCTTAAAGTCTATCAATGGTAGATCATATCGCATCGCTTATAGAAATATTAGAGACTAGACTATCACCAATAGATTTTACTATGTTTGTAATTCTTTTAAAATGTTACTATAAACTTAATTATTAGTCCTAAAAAGTGCTGCCGGGAACATATTGAACATATCTCTTATACAAAGAATCTAATATGTATATGATGTCAACATTCAGGAGAAGGGTACAGCAAAGTTCCTGTATGGGATTGATGACATTAATGATGCAGATGAACTCATCATTGTAATCCACGTTTTATACCTAATCTTTTAAGGTTCTATTTGATATGTTAATAGCTTTTGCCATCCATTTTCCGTAATATTCTGTATATACTGTTGAAGGTTGAAGGTGAAATGGATAAACTTTCAGTTGAAGAAGCTGGTTTCCTGAATTGTATTAGTGTTCCTGGTGGTGCCCCAAACAAGGTTTCAACTGATACAGTTCCACCCATTGAAAAGGTTATATTCTATACAAATACTTCTATCATGTCAGTCTTCTGTGCTTTGCATTCTTCTTAACTGCCTCAGTTATGCTTCTAGTTATCCATTTTCCCCTTTCCTTTGAAACAAAACTTTTAGTTGATATTTGAAAAGTTAGCCAAGGGTTGTCACTACAGCAACATCATATAAGAAATGCTCTATACCCTTTTTCAACCAGAAACCAACTAAGAACAAGAAACATCTAGACCCTATTTGATAGTCATTCTGTTCTTTCTTTTTAGTTTTAAAAAATTAAAGTCTATAAACATTTTTTCATCTTTAAATTATTATTTTGTCATCTACTCTGAAAGATGTATTATAAAACCAAGTCGAAATTTGAAAACTAAAAAAACGAACTTATAATTTTTAAATAATCTTGACCAAAAATAACATAATTTTTGTATTTAATAAAAAACGATTCGCATCATTCTAAAACATTCAAAGGAAATAGGCTTAATTTTAAAAAACCAAAAAGGAAGTTACCATACAGGAATCGAGTAAATTATTAATAAACATTCAAATTAGAGATTTTATTACATAACACATATAATCCATAGATAATTCATTGATAATTTCAAAAACTTTTGGTTTTTGAAAATTGTACTTTTGAAGAATTTGTTTAACTACACATTCAATTGATTTCTTGATCAAATTCTATAGAGAGAAGTTGAAAAACCACATTTTTTACCCTTTTCAAATTTTGGATTGGATTTTTGGAAACCTCCATAAAAGTTAGATAATAATTTTTTTTAAAAAAACAATAGTTAGAACTTGGAAGTGTTATGCTTCAAAACGAAATAGTTATCACATGGATAAGACTAAAGAAGTATGTCTAACCTTTAGTTTTTGTGATTATATATATAGTTTACAATTTCATCATTGTTGTTTTTTAATTTATATTGTTTGACATCTACAAACTTTTCAATTAGTGTACTTCAACATAATCTCTATGCATTGCATGGATGAATTCAGGATACTGCATACCAATATTTGTGGAGCTGCAAAGACTACGTAGATAAGGTATAACATTGTGCCTGAAATTAGTAAAGGATAACAAAGTGGTTTTTAGGAATATATCGTTAGATAATCATTTTGCTTAACCTACAAGGTTTCCCGCGTTATCTTGGCCACTGATAGCGATGTACCGGGGCAAGCTTTGGCAGAAGAGCTAGCACGTCGTCTTGGAAAAAATAGGTCTCAACTCATGTGTGAATTGGTTTGATAATCTTATAAATTAAGATCAAACTTTTATATATTTGACAAAATGAACCTAGCTCAACTGACATTAAACTGAAACTATCAAGCTCGAGATTAAATGTTTGATTATTCTCTTGACATGGATAAAATAACATGACTTTGTTTTATGAATTAACAGATGTTCGCGAGTATATTGGCCATATAAAGATGGCTTCAACCGTTTTAAGGATGCCAATGAGGTTACCTTCCTCCTTTCAATAATTATTCATAAAATTTTTGCACCTCTAAAAGTTGCTTGTGAATTGTATATAATAGGAATGAATCACGTCGATTATTTTATTATTATTTTTTTTTTGTATAATAAGAGGTGGGGATTTGAACCATAGATCACTTTGAACATTAACACATACTTTATGCTAGTTGAATTAGTTCACTTTAATAAAAAATTTAGCATCGTGTGAATTCTTGTTATTATAATCTTTACTCCCTTATTCTCTCATTAATACAATGTTAATTCCAAGCTCTTTTGCCATCAAGAGCTAAACTCAACAGACGTAGGTATGTGTTTGAGAATCAAGAGATCTTAGGTTCAAATTCTCACCATGTTTGTACTTACAAAAACTTTAAACAAAAATTCTAAATTTCTTTCTTTCATATAATCCTTTTTCTTCGTTTCACTTTTTTTTTTTATCTTATACACTATTAAATGTGATATTTTAAAAATATATAACAAAATAAATCAAAATATTTATAAAACATAGCAAAATTTTATTAATAAAAATTTATCAATATGTATAATCGATAGAATTTGACATTTTATTATATTTTAAAAATATTTTCAAATGTTATTTACACAAATTTTCCACATTAAATTATATTTATATACATACATATATATATATATACAGGTTCTAGTGCACTTGGGACCAGATGCTTTGAAGAAAGCAATTGAAGATGCCAAGTTTTACCAACTCTCAAGTTCAAATCAAATACAAAAAGGTCTGTATTTAAATATTTTAATAATTCTTCCCAATATATTCATTGGATGGCATATATTTAACCTTCATTTGAAAACAAATCCCTTAACTTTTCCATGGGTATTTTTTTTTAAAGAAAAGTTGTTTTAAATATAATTATAGGTTGAATATAATCTATATAAACTTAGATAGATATCTATTAGTGTCTATTCTAAATTTGTTGTAATCAATTACGTGTAGTTATATTTATCAATAAGTTAGTTATAATCAGTTTTATTTTTTAATTTGAAAACATCCATATTTTATATTGTATTTTAATGTGAGTAGCGTTTTTTTTTTATGTTTCTACCTTCTTATTTGAAAAACATATCTATGCCATTGCATTATTCATTGCAATGACATATATATATACACACACGTTTCTAGATTTGTTTTGCTTTCCATTTTACTTCATGGTCCCTTTTATTTTTATTATTTATTTTGATGACCTTTTCGTCATTTGACTTTGCAGAAGAGGACCTTTTCCAAGAGCATCGAGTGGAGTAATCCATAATAGTTAGGTTTTTAGTAGTTGCACTAGAGAGATATTGTGTGTTAATTCTTTGATAGGTAAGAAGCCTTGGATTAAATATTAAGGAAAAAAG

mRNA sequence

AGAAAACTTTGTGTGTGTATGCGAAGCAGATAAGGCTGCTTCCACTCTGCTCTTCCATTTCTCTCCAAATGCCAATAAACTCAACTTTCACTTTCAATTCTAATTTCAACTTCCAATTCAGATTCATTAATCATCGCTCTTTCCCTTTCTCTCATTCTCTAAACAATTTCTCCATTTCCTCTTCCCCTTCCCGATTCCTTCGGTTCTGCACTACCAGACTATCTTCCGCCGTTTCTCATGCTCCCCCCTCCCCGCCCCCAGTGAAGCATCTTATGGAGAGGATGGATCTTGTTGGAATAGTCTGTGATGAACATTGTATCCCTGGAAAATACTATTGCCTCTTCTGTCCCAAGTGCAAGGGTGGTCGTTTAATGGAGAGGAGCTTATCTCTTCATGTGATTCCAACTGGAGATTTTGCAATGTGGAGGTGTTTCCAGTTTGAGTGTGGTTGGGCAGGGCAGATATTTGCAGATGGCAAATTGGCATTTAATGGATTCGGCAAAATTACAAAAATGGTTGGACGGAGTTCCAAGGAAAGTTTGGTTTTGGAGCCTCTTTGTGATGAGCTAATCTCTTATTTCAGCATGAGAATGATATCCCAAGAAACCTTAGAACGGAATGTTGTTATGCAATTAGCTGGTCGTCAGGTTACTATTGCATTTACTTATCGACAAAATGGACAGCTTGTTGGTTGCAAGTACCGAACCATGGACAAGAGATTTTGGCAGGAGAAGGGTACAGCAAAGTTCCTGTATGGGATTGATGACATTAATGATGCAGATGAACTCATCATTGTTGAAGGTGAAATGGATAAACTTTCAGTTGAAGAAGCTGGTTTCCTGAATTGTATTAGTGTTCCTGGTGGTGCCCCAAACAAGGTTTCAACTGATACAGTTCCACCCATTGAAAAGGATACTGCATACCAATATTTGTGGAGCTGCAAAGACTACGTAGATAAGGTTTCCCGCGTTATCTTGGCCACTGATAGCGATGTACCGGGGCAAGCTTTGGCAGAAGAGCTAGCACGTCGTCTTGGAAAAAATAGATGTTCGCGAGTATATTGGCCATATAAAGATGGCTTCAACCGTTTTAAGGATGCCAATGAGGTTCTAGTGCACTTGGGACCAGATGCTTTGAAGAAAGCAATTGAAGATGCCAAGTTTTACCAACTCTCAAGTTCAAATCAAATACAAAAAGAAGAGGACCTTTTCCAAGAGCATCGAGTGGAGTAATCCATAATAGTTAGGTTTTTAGTAGTTGCACTAGAGAGATATTGTGTGTTAATTCTTTGATAGGTAAGAAGCCTTGGATTAAATATTAAGGAAAAAAG

Coding sequence (CDS)

ATGCCAATAAACTCAACTTTCACTTTCAATTCTAATTTCAACTTCCAATTCAGATTCATTAATCATCGCTCTTTCCCTTTCTCTCATTCTCTAAACAATTTCTCCATTTCCTCTTCCCCTTCCCGATTCCTTCGGTTCTGCACTACCAGACTATCTTCCGCCGTTTCTCATGCTCCCCCCTCCCCGCCCCCAGTGAAGCATCTTATGGAGAGGATGGATCTTGTTGGAATAGTCTGTGATGAACATTGTATCCCTGGAAAATACTATTGCCTCTTCTGTCCCAAGTGCAAGGGTGGTCGTTTAATGGAGAGGAGCTTATCTCTTCATGTGATTCCAACTGGAGATTTTGCAATGTGGAGGTGTTTCCAGTTTGAGTGTGGTTGGGCAGGGCAGATATTTGCAGATGGCAAATTGGCATTTAATGGATTCGGCAAAATTACAAAAATGGTTGGACGGAGTTCCAAGGAAAGTTTGGTTTTGGAGCCTCTTTGTGATGAGCTAATCTCTTATTTCAGCATGAGAATGATATCCCAAGAAACCTTAGAACGGAATGTTGTTATGCAATTAGCTGGTCGTCAGGTTACTATTGCATTTACTTATCGACAAAATGGACAGCTTGTTGGTTGCAAGTACCGAACCATGGACAAGAGATTTTGGCAGGAGAAGGGTACAGCAAAGTTCCTGTATGGGATTGATGACATTAATGATGCAGATGAACTCATCATTGTTGAAGGTGAAATGGATAAACTTTCAGTTGAAGAAGCTGGTTTCCTGAATTGTATTAGTGTTCCTGGTGGTGCCCCAAACAAGGTTTCAACTGATACAGTTCCACCCATTGAAAAGGATACTGCATACCAATATTTGTGGAGCTGCAAAGACTACGTAGATAAGGTTTCCCGCGTTATCTTGGCCACTGATAGCGATGTACCGGGGCAAGCTTTGGCAGAAGAGCTAGCACGTCGTCTTGGAAAAAATAGATGTTCGCGAGTATATTGGCCATATAAAGATGGCTTCAACCGTTTTAAGGATGCCAATGAGGTTCTAGTGCACTTGGGACCAGATGCTTTGAAGAAAGCAATTGAAGATGCCAAGTTTTACCAACTCTCAAGTTCAAATCAAATACAAAAAGAAGAGGACCTTTTCCAAGAGCATCGAGTGGAGTAA

Protein sequence

MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPPSPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSRVILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAIEDAKFYQLSSSNQIQKEEDLFQEHRVE*

Homology

[Close]

BLAST of CSPI01G25280 vs. ExPASy Swiss-Prot
Match: B5X582 (Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g30680 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.8e-87
Identity = 158/289 (54.67%), Postives = 205/289 (70.93%), Query Frame = 0


Query: 81  EHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQFECGWAGQIFADGKLAF 140
           E+C PG++  L CP C+GG   E+SLSL + P G  A W CF+ +CG  G + ADG LA 
Sbjct: 124 ENCPPGQHSGLICPTCEGGNSGEKSLSLFIAPDGSSATWNCFRGKCGLKGGVRADGGLA- 183

Query: 141 NGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQ-LAGRQVTIAFT 200
                I K+  + + E + LEPLCDE+  YF+ R IS++TLERN VMQ   G ++ IAFT
Sbjct: 184 -SADPIEKVERKITVEGIELEPLCDEIQDYFAARAISRKTLERNRVMQKRIGDEIVIAFT 243

Query: 201 YRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLN 260
           Y Q G+LV CKYR++ K F+QE+ T + LYG+DDI    E+IIVEGE+DKL++EEAGFLN
Sbjct: 244 YWQRGELVSCKYRSLTKMFFQERKTRRILYGLDDIEKTSEVIIVEGEIDKLAMEEAGFLN 303

Query: 261 CISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSRVILATDSDVPGQALAEELA 320
           C+SVP GAP KVS+  +P  +KDT Y++LW+C DY+ K SR+++ATD D PGQA+AEE+A
Sbjct: 304 CVSVPDGAPAKVSSKEIPSEDKDTKYKFLWNCNDYLKKASRIVIATDGDGPGQAMAEEIA 363

Query: 321 RRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAIEDAKFYQL 369
           RRLGK RC RV WP K     FKDANEVL+  GP  LK+AI DA+ Y +
Sbjct: 364 RRLGKERCWRVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILDAEPYPI 410

[Close]

BLAST of CSPI01G25280 vs. ExPASy Swiss-Prot
Match: F4I6E6 (Primase homolog protein OS=Arabidopsis thaliana OX=3702 GN=At1g30660 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 9.5e-84
Identity = 159/305 (52.13%), Postives = 209/305 (68.52%), Query Frame = 0


Query: 68  LMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQFECG 127
           LM ++   GI   ++C PG   CL CPKC+ G   E+SL+L++ P G  A W C + +CG
Sbjct: 38  LMRKLSEQGIDA-QNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC-RRKCG 97

Query: 128 WAGQIFADGKL-AFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVV 187
             G +  DGKL + +  GK+ + +   + ES+ LEPLCDE+  +F+ R IS +TLERN V
Sbjct: 98  LKGVLQVDGKLVSKDPIGKVERKI---TVESIKLEPLCDEIQDFFAARAISGKTLERNRV 157

Query: 188 MQ-LAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEG 247
           MQ     ++ IAFTY Q G+LV CKYR++ K+F QE+ T K LYG+DDI +  E+IIVEG
Sbjct: 158 MQKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEIIIVEG 217

Query: 248 EMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSRVILAT 307
           E DKL++EEAGF NC+SVP GAP  VS+  +P   KDTA++Y+W+C DY+ K SR+++AT
Sbjct: 218 EPDKLAMEEAGFFNCVSVPDGAPETVSSKEIPSESKDTAFKYIWNCNDYLKKASRIVIAT 277

Query: 308 DSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAIEDAKF 367
           D D PGQALAEELARRLGK RC  V WP K     FKDANEVL+  GP  LK+AI +A+ 
Sbjct: 278 DGDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILNAEP 337

Query: 368 YQLSS 371
           Y L S
Sbjct: 338 YPLKS 337

[Close]

BLAST of CSPI01G25280 vs. ExPASy TrEMBL
Match: A0A0A0LYN4 (Toprim domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G542430 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 4.4e-225
Identity = 383/387 (98.97%), Postives = 385/387 (99.48%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP 60
           MPINSTFTFNSNFNFQF+FINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP
Sbjct: 1   MPINSTFTFNSNFNFQFKFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP 60

Query: 61  SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR 120
           SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR
Sbjct: 61  SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR 120

Query: 121 CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET 180
           CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET
Sbjct: 121 CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET 180

Query: 181 LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL 240
           LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL
Sbjct: 181 LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL 240

Query: 241 IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSR 300
           IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDY+DKVSR
Sbjct: 241 IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYLDKVSR 300

Query: 301 VILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAI 360
           VILATDSDVPGQALAEELARRLGKNRC RV WPYKDGFNRFKDANEVLVHLGPDALKKAI
Sbjct: 301 VILATDSDVPGQALAEELARRLGKNRCWRVSWPYKDGFNRFKDANEVLVHLGPDALKKAI 360

Query: 361 EDAKFYQLSSSNQIQKEEDLFQEHRVE 388
           EDAKFYQLSSSNQIQKEEDLFQEHRVE
Sbjct: 361 EDAKFYQLSSSNQIQKEEDLFQEHRVE 387

[Close]

BLAST of CSPI01G25280 vs. ExPASy TrEMBL
Match: A0A1S3BDI8 (primase homolog protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 8.9e-202
Identity = 348/385 (90.39%), Postives = 363/385 (94.29%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRF-LRFCTTRLSSAVSHAP 60
           MPINSTFTFNSNFNF F+FI HRS P S SLNNFSISSSPSRF LRFCTTRLSSA+SHAP
Sbjct: 1   MPINSTFTFNSNFNFNFKFI-HRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHAP 60

Query: 61  PSPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120
           P PPPVK L E+MDL+GIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW
Sbjct: 61  PLPPPVKVLREKMDLIGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120

Query: 121 RCFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180
           RCF+FECGWAG+IFADG+LAFNGF K  KMVGRSSKESLVLEPLCDELISYFSMRMISQE
Sbjct: 121 RCFRFECGWAGRIFADGRLAFNGFSKTEKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180

Query: 181 TLERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADE 240
           TLERNVVMQ+ GRQV IAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADE
Sbjct: 181 TLERNVVMQVVGRQVAIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADE 240

Query: 241 LIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVS 300
           LIIVEGEMDKLSVEEAGFLNCISVPGGAP+KVSTDT+PPIEKD+AYQYLW CKDY+DKVS
Sbjct: 241 LIIVEGEMDKLSVEEAGFLNCISVPGGAPSKVSTDTIPPIEKDSAYQYLWGCKDYLDKVS 300

Query: 301 RVILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKA 360
           R+ILATDSDVPGQALAEELARRLGK+RC RV WPYKDGFNRFKDANEVLVHLGPDALK A
Sbjct: 301 RIILATDSDVPGQALAEELARRLGKHRCWRVDWPYKDGFNRFKDANEVLVHLGPDALKNA 360

Query: 361 IEDAKFYQLSSSNQIQKEEDLFQEH 385
           I+DAKFYQLS S  IQKEE LFQEH
Sbjct: 361 IQDAKFYQLSISISIQKEEGLFQEH 384

[Close]

BLAST of CSPI01G25280 vs. ExPASy TrEMBL
Match: A0A1S4DTX2 (primase homolog protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.2e-200
Identity = 348/386 (90.16%), Postives = 363/386 (94.04%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRF-LRFCTTRLSSAVSHAP 60
           MPINSTFTFNSNFNF F+FI HRS P S SLNNFSISSSPSRF LRFCTTRLSSA+SHAP
Sbjct: 1   MPINSTFTFNSNFNFNFKFI-HRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHAP 60

Query: 61  PSPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120
           P PPPVK L E+MDL+GIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW
Sbjct: 61  PLPPPVKVLREKMDLIGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120

Query: 121 RCFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180
           RCF+FECGWAG+IFADG+LAFNGF K  KMVGRSSKESLVLEPLCDELISYFSMRMISQE
Sbjct: 121 RCFRFECGWAGRIFADGRLAFNGFSKTEKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180

Query: 181 TLERNVVMQLAGR-QVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD 240
           TLERNVVMQ+ GR QV IAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD
Sbjct: 181 TLERNVVMQVVGRQQVAIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD 240

Query: 241 ELIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKV 300
           ELIIVEGEMDKLSVEEAGFLNCISVPGGAP+KVSTDT+PPIEKD+AYQYLW CKDY+DKV
Sbjct: 241 ELIIVEGEMDKLSVEEAGFLNCISVPGGAPSKVSTDTIPPIEKDSAYQYLWGCKDYLDKV 300

Query: 301 SRVILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKK 360
           SR+ILATDSDVPGQALAEELARRLGK+RC RV WPYKDGFNRFKDANEVLVHLGPDALK 
Sbjct: 301 SRIILATDSDVPGQALAEELARRLGKHRCWRVDWPYKDGFNRFKDANEVLVHLGPDALKN 360

Query: 361 AIEDAKFYQLSSSNQIQKEEDLFQEH 385
           AI+DAKFYQLS S  IQKEE LFQEH
Sbjct: 361 AIQDAKFYQLSISISIQKEEGLFQEH 385

[Close]

BLAST of CSPI01G25280 vs. ExPASy TrEMBL
Match: A0A1S4DT34 (primase homolog protein isoform X3 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.1e-187
Identity = 335/386 (86.79%), Postives = 348/386 (90.16%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRF-LRFCTTRLSSAVSHAP 60
           MPINSTFTFNSNFNF F+FI HRS P S SLNNFSISSSPSRF LRFCTTRLSSA+SHAP
Sbjct: 1   MPINSTFTFNSNFNFNFKFI-HRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHAP 60

Query: 61  PSPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120
           P PPPVK L E+MDL+GIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW
Sbjct: 61  PLPPPVKVLREKMDLIGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120

Query: 121 RCFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180
           RCF+FECGWAG+IFADG+LAFNGF K  KMVGRSSKESLVLEPLCDELISYFSMRMISQE
Sbjct: 121 RCFRFECGWAGRIFADGRLAFNGFSKTEKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180

Query: 181 TLERNVVMQLAGR-QVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD 240
           TLERNVVMQ+ GR QV IAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD
Sbjct: 181 TLERNVVMQVVGRQQVAIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD 240

Query: 241 ELIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKV 300
           ELIIVEGEMDKLSVEEAGFLNCISVPGGAP+KVSTDT+PPIE                KV
Sbjct: 241 ELIIVEGEMDKLSVEEAGFLNCISVPGGAPSKVSTDTIPPIE----------------KV 300

Query: 301 SRVILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKK 360
           SR+ILATDSDVPGQALAEELARRLGK+RC RV WPYKDGFNRFKDANEVLVHLGPDALK 
Sbjct: 301 SRIILATDSDVPGQALAEELARRLGKHRCWRVDWPYKDGFNRFKDANEVLVHLGPDALKN 360

Query: 361 AIEDAKFYQLSSSNQIQKEEDLFQEH 385
           AI+DAKFYQLS S  IQKEE LFQEH
Sbjct: 361 AIQDAKFYQLSISISIQKEEGLFQEH 369

[Close]

BLAST of CSPI01G25280 vs. ExPASy TrEMBL
Match: A0A1S4DT27 (primase homolog protein isoform X4 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.6e-182
Identity = 316/348 (90.80%), Postives = 330/348 (94.83%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRF-LRFCTTRLSSAVSHAP 60
           MPINSTFTFNSNFNF F+FI HRS P S SLNNFSISSSPSRF LRFCTTRLSSA+SHAP
Sbjct: 1   MPINSTFTFNSNFNFNFKFI-HRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHAP 60

Query: 61  PSPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120
           P PPPVK L E+MDL+GIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW
Sbjct: 61  PLPPPVKVLREKMDLIGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120

Query: 121 RCFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180
           RCF+FECGWAG+IFADG+LAFNGF K  KMVGRSSKESLVLEPLCDELISYFSMRMISQE
Sbjct: 121 RCFRFECGWAGRIFADGRLAFNGFSKTEKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180

Query: 181 TLERNVVMQLAGR-QVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD 240
           TLERNVVMQ+ GR QV IAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD
Sbjct: 181 TLERNVVMQVVGRQQVAIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD 240

Query: 241 ELIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKV 300
           ELIIVEGEMDKLSVEEAGFLNCISVPGGAP+KVSTDT+PPIEKD+AYQYLW CKDY+DKV
Sbjct: 241 ELIIVEGEMDKLSVEEAGFLNCISVPGGAPSKVSTDTIPPIEKDSAYQYLWGCKDYLDKV 300

Query: 301 SRVILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANE 347
           SR+ILATDSDVPGQALAEELARRLGK+RC RV WPYKDGFNRFKDANE
Sbjct: 301 SRIILATDSDVPGQALAEELARRLGKHRCWRVDWPYKDGFNRFKDANE 347

[Close]

BLAST of CSPI01G25280 vs. NCBI nr
Match: XP_011659247.1 (primase homolog protein isoform X1 [Cucumis sativus] >KGN65952.1 hypothetical protein Csa_023223 [Cucumis sativus])

HSP 1 Score: 790.0 bits (2039), Expect = 9.1e-225
Identity = 383/387 (98.97%), Postives = 385/387 (99.48%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP 60
           MPINSTFTFNSNFNFQF+FINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP
Sbjct: 1   MPINSTFTFNSNFNFQFKFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP 60

Query: 61  SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR 120
           SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR
Sbjct: 61  SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR 120

Query: 121 CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET 180
           CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET
Sbjct: 121 CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET 180

Query: 181 LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL 240
           LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL
Sbjct: 181 LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL 240

Query: 241 IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSR 300
           IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDY+DKVSR
Sbjct: 241 IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYLDKVSR 300

Query: 301 VILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAI 360
           VILATDSDVPGQALAEELARRLGKNRC RV WPYKDGFNRFKDANEVLVHLGPDALKKAI
Sbjct: 301 VILATDSDVPGQALAEELARRLGKNRCWRVSWPYKDGFNRFKDANEVLVHLGPDALKKAI 360

Query: 361 EDAKFYQLSSSNQIQKEEDLFQEHRVE 388
           EDAKFYQLSSSNQIQKEEDLFQEHRVE
Sbjct: 361 EDAKFYQLSSSNQIQKEEDLFQEHRVE 387

[Close]

BLAST of CSPI01G25280 vs. NCBI nr
Match: XP_031736131.1 (primase homolog protein isoform X3 [Cucumis sativus])

HSP 1 Score: 726.9 bits (1875), Expect = 9.4e-206
Identity = 356/387 (91.99%), Postives = 358/387 (92.51%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP 60
           MPINSTFTFNSNFNFQF+FINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP
Sbjct: 1   MPINSTFTFNSNFNFQFKFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP 60

Query: 61  SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR 120
           SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR
Sbjct: 61  SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR 120

Query: 121 CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET 180
           CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDE              
Sbjct: 121 CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDE-------------- 180

Query: 181 LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL 240
                        VTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL
Sbjct: 181 -------------VTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL 240

Query: 241 IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSR 300
           IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDY+DKVSR
Sbjct: 241 IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYLDKVSR 300

Query: 301 VILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAI 360
           VILATDSDVPGQALAEELARRLGKNRC RV WPYKDGFNRFKDANEVLVHLGPDALKKAI
Sbjct: 301 VILATDSDVPGQALAEELARRLGKNRCWRVSWPYKDGFNRFKDANEVLVHLGPDALKKAI 360

Query: 361 EDAKFYQLSSSNQIQKEEDLFQEHRVE 388
           EDAKFYQLSSSNQIQKEEDLFQEHRVE
Sbjct: 361 EDAKFYQLSSSNQIQKEEDLFQEHRVE 360

[Close]

BLAST of CSPI01G25280 vs. NCBI nr
Match: XP_008445790.1 (PREDICTED: primase homolog protein isoform X2 [Cucumis melo])

HSP 1 Score: 712.6 bits (1838), Expect = 1.8e-201
Identity = 348/385 (90.39%), Postives = 363/385 (94.29%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRF-LRFCTTRLSSAVSHAP 60
           MPINSTFTFNSNFNF F+FI HRS P S SLNNFSISSSPSRF LRFCTTRLSSA+SHAP
Sbjct: 1   MPINSTFTFNSNFNFNFKFI-HRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHAP 60

Query: 61  PSPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120
           P PPPVK L E+MDL+GIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW
Sbjct: 61  PLPPPVKVLREKMDLIGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120

Query: 121 RCFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180
           RCF+FECGWAG+IFADG+LAFNGF K  KMVGRSSKESLVLEPLCDELISYFSMRMISQE
Sbjct: 121 RCFRFECGWAGRIFADGRLAFNGFSKTEKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180

Query: 181 TLERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADE 240
           TLERNVVMQ+ GRQV IAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADE
Sbjct: 181 TLERNVVMQVVGRQVAIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADE 240

Query: 241 LIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVS 300
           LIIVEGEMDKLSVEEAGFLNCISVPGGAP+KVSTDT+PPIEKD+AYQYLW CKDY+DKVS
Sbjct: 241 LIIVEGEMDKLSVEEAGFLNCISVPGGAPSKVSTDTIPPIEKDSAYQYLWGCKDYLDKVS 300

Query: 301 RVILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKA 360
           R+ILATDSDVPGQALAEELARRLGK+RC RV WPYKDGFNRFKDANEVLVHLGPDALK A
Sbjct: 301 RIILATDSDVPGQALAEELARRLGKHRCWRVDWPYKDGFNRFKDANEVLVHLGPDALKNA 360

Query: 361 IEDAKFYQLSSSNQIQKEEDLFQEH 385
           I+DAKFYQLS S  IQKEE LFQEH
Sbjct: 361 IQDAKFYQLSISISIQKEEGLFQEH 384

[Close]

BLAST of CSPI01G25280 vs. NCBI nr
Match: XP_016899150.1 (PREDICTED: primase homolog protein isoform X1 [Cucumis melo])

HSP 1 Score: 708.0 bits (1826), Expect = 4.5e-200
Identity = 348/386 (90.16%), Postives = 363/386 (94.04%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRF-LRFCTTRLSSAVSHAP 60
           MPINSTFTFNSNFNF F+FI HRS P S SLNNFSISSSPSRF LRFCTTRLSSA+SHAP
Sbjct: 1   MPINSTFTFNSNFNFNFKFI-HRSSPSSLSLNNFSISSSPSRFLLRFCTTRLSSALSHAP 60

Query: 61  PSPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120
           P PPPVK L E+MDL+GIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW
Sbjct: 61  PLPPPVKVLREKMDLIGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMW 120

Query: 121 RCFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180
           RCF+FECGWAG+IFADG+LAFNGF K  KMVGRSSKESLVLEPLCDELISYFSMRMISQE
Sbjct: 121 RCFRFECGWAGRIFADGRLAFNGFSKTEKMVGRSSKESLVLEPLCDELISYFSMRMISQE 180

Query: 181 TLERNVVMQLAGR-QVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD 240
           TLERNVVMQ+ GR QV IAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD
Sbjct: 181 TLERNVVMQVVGRQQVAIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDAD 240

Query: 241 ELIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKV 300
           ELIIVEGEMDKLSVEEAGFLNCISVPGGAP+KVSTDT+PPIEKD+AYQYLW CKDY+DKV
Sbjct: 241 ELIIVEGEMDKLSVEEAGFLNCISVPGGAPSKVSTDTIPPIEKDSAYQYLWGCKDYLDKV 300

Query: 301 SRVILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKK 360
           SR+ILATDSDVPGQALAEELARRLGK+RC RV WPYKDGFNRFKDANEVLVHLGPDALK 
Sbjct: 301 SRIILATDSDVPGQALAEELARRLGKHRCWRVDWPYKDGFNRFKDANEVLVHLGPDALKN 360

Query: 361 AIEDAKFYQLSSSNQIQKEEDLFQEH 385
           AI+DAKFYQLS S  IQKEE LFQEH
Sbjct: 361 AIQDAKFYQLSISISIQKEEGLFQEH 385

[Close]

BLAST of CSPI01G25280 vs. NCBI nr
Match: XP_031736130.1 (primase homolog protein isoform X2 [Cucumis sativus])

HSP 1 Score: 698.0 bits (1800), Expect = 4.7e-197
Identity = 353/387 (91.21%), Postives = 360/387 (93.02%), Query Frame = 0


Query: 1   MPINSTFTFNSNFNFQFRFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP 60
           MPINSTFTFNSNFNFQF+FINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP
Sbjct: 1   MPINSTFTFNSNFNFQFKFINHRSFPFSHSLNNFSISSSPSRFLRFCTTRLSSAVSHAPP 60

Query: 61  SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWR 120
           SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPK    RL  +  S +    G+  +  
Sbjct: 61  SPPPVKHLMERMDLVGIVCDEHCIPGKYYCLFCPKI-CSRLTVQGWSFN----GEELISS 120

Query: 121 CFQFECGWAGQIFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET 180
           C   +  W   IFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET
Sbjct: 121 C---DSNW---IFADGKLAFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQET 180

Query: 181 LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL 240
           LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL
Sbjct: 181 LERNVVMQLAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADEL 240

Query: 241 IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSR 300
           IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDY+DKVSR
Sbjct: 241 IIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYLDKVSR 300

Query: 301 VILATDSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAI 360
           VILATDSDVPGQALAEELARRLGKNRC RV WPYKDGFNRFKDANEVLVHLGPDALKKAI
Sbjct: 301 VILATDSDVPGQALAEELARRLGKNRCWRVSWPYKDGFNRFKDANEVLVHLGPDALKKAI 360

Query: 361 EDAKFYQLSSSNQIQKEEDLFQEHRVE 388
           EDAKFYQLSSSNQIQKEEDLFQEHRVE
Sbjct: 361 EDAKFYQLSSSNQIQKEEDLFQEHRVE 376

[Close]

BLAST of CSPI01G25280 vs. TAIR 10
Match: AT1G30680.1 (toprim domain-containing protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.3e-88
Identity = 158/289 (54.67%), Postives = 205/289 (70.93%), Query Frame = 0


Query: 81  EHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQFECGWAGQIFADGKLAF 140
           E+C PG++  L CP C+GG   E+SLSL + P G  A W CF+ +CG  G + ADG LA 
Sbjct: 124 ENCPPGQHSGLICPTCEGGNSGEKSLSLFIAPDGSSATWNCFRGKCGLKGGVRADGGLA- 183

Query: 141 NGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVVMQ-LAGRQVTIAFT 200
                I K+  + + E + LEPLCDE+  YF+ R IS++TLERN VMQ   G ++ IAFT
Sbjct: 184 -SADPIEKVERKITVEGIELEPLCDEIQDYFAARAISRKTLERNRVMQKRIGDEIVIAFT 243

Query: 201 YRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEGEMDKLSVEEAGFLN 260
           Y Q G+LV CKYR++ K F+QE+ T + LYG+DDI    E+IIVEGE+DKL++EEAGFLN
Sbjct: 244 YWQRGELVSCKYRSLTKMFFQERKTRRILYGLDDIEKTSEVIIVEGEIDKLAMEEAGFLN 303

Query: 261 CISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSRVILATDSDVPGQALAEELA 320
           C+SVP GAP KVS+  +P  +KDT Y++LW+C DY+ K SR+++ATD D PGQA+AEE+A
Sbjct: 304 CVSVPDGAPAKVSSKEIPSEDKDTKYKFLWNCNDYLKKASRIVIATDGDGPGQAMAEEIA 363

Query: 321 RRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAIEDAKFYQL 369
           RRLGK RC RV WP K     FKDANEVL+  GP  LK+AI DA+ Y +
Sbjct: 364 RRLGKERCWRVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILDAEPYPI 410

[Close]

BLAST of CSPI01G25280 vs. TAIR 10
Match: AT1G30660.1 (nucleic acid binding;nucleic acid binding )

HSP 1 Score: 312.0 bits (798), Expect = 6.7e-85
Identity = 159/305 (52.13%), Postives = 209/305 (68.52%), Query Frame = 0


Query: 68  LMERMDLVGIVCDEHCIPGKYYCLFCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQFECG 127
           LM ++   GI   ++C PG   CL CPKC+ G   E+SL+L++ P G  A W C + +CG
Sbjct: 38  LMRKLSEQGIDA-QNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC-RRKCG 97

Query: 128 WAGQIFADGKL-AFNGFGKITKMVGRSSKESLVLEPLCDELISYFSMRMISQETLERNVV 187
             G +  DGKL + +  GK+ + +   + ES+ LEPLCDE+  +F+ R IS +TLERN V
Sbjct: 98  LKGVLQVDGKLVSKDPIGKVERKI---TVESIKLEPLCDEIQDFFAARAISGKTLERNRV 157

Query: 188 MQ-LAGRQVTIAFTYRQNGQLVGCKYRTMDKRFWQEKGTAKFLYGIDDINDADELIIVEG 247
           MQ     ++ IAFTY Q G+LV CKYR++ K+F QE+ T K LYG+DDI +  E+IIVEG
Sbjct: 158 MQKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEIIIVEG 217

Query: 248 EMDKLSVEEAGFLNCISVPGGAPNKVSTDTVPPIEKDTAYQYLWSCKDYVDKVSRVILAT 307
           E DKL++EEAGF NC+SVP GAP  VS+  +P   KDTA++Y+W+C DY+ K SR+++AT
Sbjct: 218 EPDKLAMEEAGFFNCVSVPDGAPETVSSKEIPSESKDTAFKYIWNCNDYLKKASRIVIAT 277

Query: 308 DSDVPGQALAEELARRLGKNRCSRVYWPYKDGFNRFKDANEVLVHLGPDALKKAIEDAKF 367
           D D PGQALAEELARRLGK RC  V WP K     FKDANEVL+  GP  LK+AI +A+ 
Sbjct: 278 DGDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKDANEVLMSKGPHLLKEAILNAEP 337

Query: 368 YQLSS 371
           Y L S
Sbjct: 338 YPLKS 337

The following BLAST results are available for this feature:

BLAST of CSPI01G25280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5X582	1.8e-87	54.67	Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=...	[more]
F4I6E6	9.5e-84	52.13	Primase homolog protein OS=Arabidopsis thaliana OX=3702 GN=At1g30660 PE=3 SV=1	[more]

BLAST of CSPI01G25280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYN4	4.4e-225	98.97	Toprim domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G542430 PE=4...	[more]
A0A1S3BDI8	8.9e-202	90.39	primase homolog protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 ...	[more]
A0A1S4DTX2	2.2e-200	90.16	primase homolog protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 ...	[more]
A0A1S4DT34	2.1e-187	86.79	primase homolog protein isoform X3 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 ...	[more]
A0A1S4DT27	1.6e-182	90.80	primase homolog protein isoform X4 OS=Cucumis melo OX=3656 GN=LOC103488713 PE=4 ...	[more]

BLAST of CSPI01G25280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659247.1	9.1e-225	98.97	primase homolog protein isoform X1 [Cucumis sativus] >KGN65952.1 hypothetical pr...	[more]
XP_031736131.1	9.4e-206	91.99	primase homolog protein isoform X3 [Cucumis sativus]	[more]
XP_008445790.1	1.8e-201	90.39	PREDICTED: primase homolog protein isoform X2 [Cucumis melo]	[more]
XP_016899150.1	4.5e-200	90.16	PREDICTED: primase homolog protein isoform X1 [Cucumis melo]	[more]
XP_031736130.1	4.7e-197	91.21	primase homolog protein isoform X2 [Cucumis sativus]	[more]

BLAST of CSPI01G25280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30680.1	1.3e-88	54.67	toprim domain-containing protein	[more]
AT1G30660.1	6.7e-85	52.13	nucleic acid binding;nucleic acid binding	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006171	TOPRIM domain	SMART	SM00493	toprim5	coord: 238..327 e-value: 1.2E-8 score: 44.7
IPR006171	TOPRIM domain	PFAM	PF13662	Toprim_4	coord: 238..330 e-value: 7.1E-19 score: 68.1
IPR006171	TOPRIM domain	PROSITE	PS50880	TOPRIM	coord: 238..337 score: 10.456876
None	No IPR available	GENE3D	3.40.1360.10		coord: 225..367 e-value: 5.0E-31 score: 109.3
None	No IPR available	PANTHER	PTHR12873:SF1	ACID-BINDING PROTEIN, PUTATIVE-RELATED	coord: 56..367
None	No IPR available	SUPERFAMILY	56731	DNA primase core	coord: 171..337
IPR027032	Twinkle-like protein	PANTHER	PTHR12873	T7-LIKE MITOCHONDRIAL DNA HELICASE	coord: 56..367
IPR034154	Archaeal primase DnaG/twinkle, TOPRIM domain	CDD	cd01029	TOPRIM_primases	coord: 238..333 e-value: 6.43308E-11 score: 56.1246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G25280.1	CSPI01G25280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
molecular_function	GO:0043139	5'-3' DNA helicase activity
molecular_function	GO:0003697	single-stranded DNA binding