CSPI01G25160 (gene) Cucumber (PI 183967) v1

BLAST of CSPI01G25160 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 3.4e-141
Identity = 247/519 (47.59%), Postives = 356/519 (68.59%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCS--SSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPIN 60
           MKF       L F    +   S   P +++  N   FLQCL      S  ++K+++TP +
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  TSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFE 120
           TS+SSVL  SI+N RFS P+ PKP+ I+TP   S VQ+AV C++  G+ IR RSGGHD+E
Sbjct: 61  TSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYE 120

Query: 121 GLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSP 180
           GLSYV + PF+I+DL +L S+T++V   + WVQ+GAT+GELYY I +K+RTLAFPAG  P
Sbjct: 121 GLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCP 180

Query: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGS 240
           TVGVGGHFSGGG+GTLLRK+GLAAD+VIDA +VDA G   +R+ MGED FWAIRGGGG S
Sbjct: 181 TVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSS 240

Query: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKI 300
           F +V++WK+ L+ VP+TVT+  +++  E+ A+K++ +WQ+VA+K+ +DLF+       ++
Sbjct: 241 FCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFI-------RV 300

Query: 301 SAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360
             Q  K    A F  L+LG    L+ ++NK FP+LGL +++C E SWIESV++     ++
Sbjct: 301 MLQRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---EL 360

Query: 361 EDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
            ++P+ VL  RT  A+   K KSD+V+EP+PK  I ++W+RL+  + E A L+F P+GG+
Sbjct: 361 GEEPINVLTKRT-RASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGK 420

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           MS+I+D E PF HR GN+Y+I YL  W     + ++ ++ W+  +Y  M+ FV+KSPR A
Sbjct: 421 MSEIADYETPFPHRKGNIYEIQYLNYW---RGDVKEKYMRWVERVYDDMSEFVAKSPRGA 480

Query: 481 YVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           Y+N RDLD+G      ++ Y+  + WG+KYF NNF R++
Sbjct: 481 YINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLV 505

BLAST of CSPI01G25160 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 7.5e-141
Identity = 250/519 (48.17%), Postives = 357/519 (68.79%), Query Frame = 0

Query: 6   LLPLSLAFIAVCSSSSVWPSASS-HVNHEEFLQCLLHHSPHSKSIAKLVYTPINTSYSSV 65
           LL L + F+ +  S S +PS S+   NHE FL+CL H      S  ++++T  + SY S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSI 66

Query: 66  LNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVA 125
           LN SI+N RF +  TPKP+ IITP   + VQ+ + C++ HG+ IR RSGGHD+EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 -YHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVG 185
              PF+++DL +L S+T++V   T WVQSGAT+GELYY I + S++LAFPAG  PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVV 245
           G F GGG+GTL+RKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGD 305
           +WK++L+ VP+ VT+  + +T E+EA+ ++++WQY+A+K+  DLF+   L          
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML--------QK 306

Query: 306 KINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPL 365
           +    A F  L+LG   +L+A++   FP+LGL    C+E SWIESV++      I+ + +
Sbjct: 307 ETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWF-----IKGESM 366

Query: 366 EVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQIS 425
           E+L  R    + + K K D+++EPIPK  I+ +W+R E+ +   A ++  P+GG+MS+I+
Sbjct: 367 EILAKR-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIA 426

Query: 426 DSEIPFSHRAGNLYKIGYLTGWFEP----GVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 485
           D+EIPF HR GNLY+I YL  W E       N EK +L W+  +Y +MTP+VSKSPR AY
Sbjct: 427 DNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEK-YLRWVESVYEFMTPYVSKSPRRAY 486

Query: 486 VNYRDLDIGSNSKYG-KTSYKRARVWGLKYFGNNFNRML 518
           VN+RD+D+G       KT Y+ A+VWG+KYF NNF+R++
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLV 508

BLAST of CSPI01G25160 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.7e-140
Identity = 243/490 (49.59%), Postives = 342/490 (69.80%), Query Frame = 0

Query: 33  EEFLQCLLHHSPHSKSIAKLVYTPINTSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNIS 92
           E FL+C   + P++ +  KLVYT  N  Y SVLN +I NLRF+   TPKPL I+TP+++S
Sbjct: 32  ENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVS 91

Query: 93  HVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQS 152
           H+Q  ++CSK  GLQIR RSGGHD EG+SY++  PF+IVDL ++ S+ I+V   TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY + EK+  L+  AG  PTV  GGHF GGG+G L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVN 211

Query: 213 ANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE-EEAIK 272
            +G   DRKSMGEDLFWA+RGGG  SFGI+VAWK++LV VP + T+ ++ + +E  E +K
Sbjct: 212 VHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIMEIHELVK 271

Query: 273 LVDQWQYVANKLDEDLFLGINLLGGKIS-AQGDKINPIALFF-SLFLGKADELMAILNKT 332
           LV++WQ +A K D+DL L  + +   I+  QG     I  +F S+FLG  D L+ ++NK+
Sbjct: 272 LVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKS 331

Query: 333 FPQLGLTKEECKETSWIESVVYTGNGLQIEDQPL--EVLLNRTPLATGNIKMKSDYVKEP 392
           FP+LG+ K +C++ SWI+++++    +  +      E+LL+R+    G  K+K DYVK+P
Sbjct: 332 FPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKP 391

Query: 393 IPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEIPFSHRAGNLYKIGYLTGWFE 452
           IP++   +I ++L  +DI        PYGG M +IS+S IPF HRAG LY++ Y+  W +
Sbjct: 392 IPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEK 451

Query: 453 PGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNSKYGKTSYKRARVWGLK 512
              N  + HLNWIR+IY +MTP+VSK+PR AY+NYRDLDIG N      +Y +AR+WG K
Sbjct: 452 QEDN--EKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGEK 511

Query: 513 YFGNNFNRML 518
           YFG NF+R++
Sbjct: 512 YFGKNFDRLV 518

BLAST of CSPI01G25160 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 8.3e-140
Identity = 244/513 (47.56%), Postives = 358/513 (69.79%), Query Frame = 0

Query: 10  SLAFIAVCSSSSVWPSASSH-VNHEEFLQCLLHHSPHSKSIAKLVYTPINTSYSSVLNFS 69
           S  F  +  S ++ P  S+H  NHE+FL+CL +    +    K+++T  ++S+ S+L+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 70  IRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYH-P 129
           I+N RFS+  TPKP+ IITP   S VQ  + C++ HG+ +R RS GH +EGLSY+AY+ P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 130 FIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFS 189
           F ++DL +L S++++V   T WVQ+GAT GELYY I + +++LAFPAG  PTVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 190 GGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKV 249
           GGG+GTLLRKYGLAADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 250 KLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGDKINP 309
           KLV VP+T+T+  + +T ++EA++++ +WQY A+K+ +DLF+   L       + +K   
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL------ERSNKNAV 304

Query: 310 IALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLL 369
            ALF  L++G  + L+A++ + FP+LGL KE C+E SWIESV++  +  + E   L VL 
Sbjct: 305 HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGES--LGVLT 364

Query: 370 NRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEI 429
           NR   +  + K K D+V+EPIP+A I+EIW+RLE+ +     ++  P+GG+MS++++ E 
Sbjct: 365 NRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 424

Query: 430 PFSHRAGNLYKIGYLTGW-FEPGVNAEKT--HLNWIRDIYGYMTPFVSKSPRAAYVNYRD 489
           PF HR GNLY+I Y+  W  E   N  +T  +L W+  +Y +MTP+VSKSPR AYVN++D
Sbjct: 425 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 490 LDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           +D+G      KT Y+  + WG+KYF NNF R++
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLV 508

BLAST of CSPI01G25160 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.8e-139
Identity = 241/490 (49.18%), Postives = 340/490 (69.39%), Query Frame = 0

Query: 33  EEFLQCLLHHSPHSKSIAKLVYTPINTSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNIS 92
           E FL+C   + P + + AKLVYT  +  Y S+LN +I+NLRF+   TPKPL IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQS 152
           H+Q  ++CSK  GLQIR RSGGHD EG+SY++  PF+IVDL ++ SV I+V   TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +  L+FPAG  PTVG GGHFSGGG+G L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTISRTLE-EEAIK 272
            +G   DRKSMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI ++ + +E  E +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LVDQWQYVANKLDEDLFLGINLLGGKIS-AQGDKINPIALFF-SLFLGKADELMAILNKT 332
           LV++WQ +A   +++L L  + +   I+  QG     I  +F S+F G  D L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPQLGLTKEECKETSWIESVVYTGNGLQIEDQ--PLEVLLNRTPLATGNIKMKSDYVKEP 392
           FP+LG+ K +CK+ SWI+++++    +         E+LL+R+        +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 IPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEIPFSHRAGNLYKIGYLTGWFE 452
           IP+  +  I ++L  +D+     VF PYGG M +IS+S IPF HRAG +Y+I Y+  W +
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 453 PGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRDLDIGSNSKYGKTSYKRARVWGLK 512
              N  + H+NWIR++Y + TP+VS++PR AY+NYRDLD+G  +     +Y +AR+WG K
Sbjct: 452 QEDN--EKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRML 518
           YFG NFNR++
Sbjct: 512 YFGKNFNRLV 519

BLAST of CSPI01G25160 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 1.2e-285
Identity = 483/517 (93.42%), Postives = 502/517 (97.10%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINTS 60
           MKFSLLLPLSLAFI VCSSSSVWPSASSHVNHEEFL CLLHHSP SKSI KLVYTPINTS
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YS VLNFSIRNLRFS PNTPKPL IITP+NISHVQAAVICSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYHPF++VDLI+LSSVTIEV+QSTAWVQSGATLGELYYRIAEKSRTLAFPAGN PTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGFGTLLRKYGLAADNVIDAY+VDANG FHDRKSMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTICTISRTLEEEAIKL+DQWQYVANKLDEDLFLGINLLGGKI A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360
           QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED
Sbjct: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360

Query: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420
           QPLEVLLNRTPLA GNIKMKSDYVKEPI KATI+E+WQRL+SQDIEGANLVF+PYGGRMS
Sbjct: 361 QPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRMS 420

Query: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480
           QIS+SEIPFSHRAGNLYKIGYLTGWFEPG+NAEKTHLNWIRDIY YMTPFV+KSPRAAYV
Sbjct: 421 QISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYV 480

Query: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           NYRDLDIGSNSKYGKTSYKRA VWG KYFG+NF+R++
Sbjct: 481 NYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLV 517

BLAST of CSPI01G25160 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 1.2e-285
Identity = 483/517 (93.42%), Postives = 502/517 (97.10%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINTS 60
           MKFSLLLPLSLAFI VCSSSSVWPSASSHVNHEEFL CLLHHSP SKSI KLVYTPINTS
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YS VLNFSIRNLRFS PNTPKPL IITP+NISHVQAAVICSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYHPF++VDLI+LSSVTIEV+QSTAWVQSGATLGELYYRIAEKSRTLAFPAGN PTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGFGTLLRKYGLAADNVIDAY+VDANG FHDRKSMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTICTISRTLEEEAIKL+DQWQYVANKLDEDLFLGINLLGGKI A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360
           QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED
Sbjct: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360

Query: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420
           QPLEVLLNRTPLA GNIKMKSDYVKEPI KATI+E+WQRL+SQDIEGANLVF+PYGGRMS
Sbjct: 361 QPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRMS 420

Query: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480
           QIS+SEIPFSHRAGNLYKIGYLTGWFEPG+NAEKTHLNWIRDIY YMTPFV+KSPRAAYV
Sbjct: 421 QISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYV 480

Query: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           NYRDLDIGSNSKYGKTSYKRA VWG KYFG+NF+R++
Sbjct: 481 NYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLV 517

BLAST of CSPI01G25160 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 3.9e-233
Identity = 397/519 (76.49%), Postives = 449/519 (86.51%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINTS 60
           M  S L+PLSL FI VCSSSS+W + SS + HEEFLQCLLHHSP + SI KLV+TPIN+S
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           +S++ NF  RN RFS PNTPKPL IITP+NISH+QAAVICSKSHGLQIRIRSGGHDFEGL
Sbjct: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYH FI+VDLI+L SVT++V+QSTAWVQSGATLGELYY+I +KSRTL FPAG  PTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           +VVAWKVKLVPVPA+VTIC+  R++EEEAIKL+D+WQYVANKL+EDLFL I   GGK+SA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360
           Q G KINPIALFF LFLG  +ELMAILNKTFP+LG TKEECKE SWIES     N  QIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEVLLNRTPLATG-NIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
           DQPLE LLNRTP+  G + K+KSDYVKEP+ K  IE IW+RLESQDIEG  L  +PYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           MS+IS+S IPFSHRAG LY+IGY+ GW E GV AE+ HLNWIR+IY YMTPFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           YVNYRDLDIGSN+KYGK +YK+A V+G KYFGNNFNR++
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLV 532

BLAST of CSPI01G25160 vs. ExPASy TrEMBL
Match: A0A5D3C1K3 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00270 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.4e-222
Identity = 382/501 (76.25%), Postives = 429/501 (85.63%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINTS 60
           M  S L+PLSL FI VCSSSS+W + SS + HEEFLQCLLHHSP + SI KLV+TPIN+S
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           +S++ NF  RN RFS PNTPKPL IITP+NISH+QAAVICSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYH FI+VDLI+L SVT++V+QSTAWVQSGATLGELYY+I +KSRTL FPAG  PTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           +VVAWKVKLVPVPA+VTIC+  R++EEEAIKL+D+WQYVANKL+EDLFL I   GGK+SA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360
           Q G KINPIALFF LFLG  +ELMAILNKTFP+LG TKEECKE SWIES     N  QIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEVLLNRTPLATG-NIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
           DQPLE LLNRTP+  G + K+KSDYVKEP+ K  IE IW+RLESQDIEG  L  +PYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           MS+IS+S IPFSHRAG LY+IGY+ GW E GV AE+ HLNWIR+IY YMTPFVSKSPR A
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 481 YVNYRDLDIGSNSKYGKTSYK 500
           YVNYRDLDIGSN+K  K   K
Sbjct: 481 YVNYRDLDIGSNNKLVKVKTK 501

BLAST of CSPI01G25160 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 3.3e-216
Identity = 377/517 (72.92%), Postives = 443/517 (85.69%), Query Frame = 0

Query: 4   SLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPH--SKSIAKLVYTPINTSY 63
           S L+ LSLAFI + SSSS+W SA  +  HE FLQCLL HS    + SI+K+++TPIN+SY
Sbjct: 5   SPLISLSLAFIFL-SSSSLWASAFPN-KHESFLQCLLDHSLEYSNYSISKVIHTPINSSY 64

Query: 64  SSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLS 123
           SSVL+FSIRNLRFS   TPKPL I+TP+++SH+QAAVICSKS+G QIR RSGGHDFEGLS
Sbjct: 65  SSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLS 124

Query: 124 YVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVG 183
           YVA+ PFIIVDLI+L S++I++K +TAWVQSGATLGELYY IA+KSRTLAFPAG  PTVG
Sbjct: 125 YVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVG 184

Query: 184 VGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGI 243
           VGGHFSGGG+G LLRKYGLAADNVIDAYLVDA+G FH+R+SMGEDLFWAIRGGGGGSFGI
Sbjct: 185 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGI 244

Query: 244 VVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQ 303
           VVAWKVKLVPVPATVT+C+ +RTLEE A+K+  +WQYVANKLDE+LFLGI  LGGKI++Q
Sbjct: 245 VVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQ 304

Query: 304 -GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 363
            G K NP+A FFSLFLGKADEL++ILN  FP+L L K++C ETSWIES V  G G   E 
Sbjct: 305 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTE- 364

Query: 364 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 423
             LE LL+RTPL   + K+KSDYVKEP+ +A ++ IW+RL+SQDIEG N++FVPYGGRMS
Sbjct: 365 LTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMS 424

Query: 424 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 483
           QIS+SE PFSHRAG LYKIGY+ GW E G++AEK HL WIR++Y YMTPFVSKSPRAAY 
Sbjct: 425 QISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYA 484

Query: 484 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           NYRDLDIGSN+K+GKTSYK+A VWGLKYFGNNFNR++
Sbjct: 485 NYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLV 518

BLAST of CSPI01G25160 vs. NCBI nr
Match: XP_011659204.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653317.1 hypothetical protein Csa_023357 [Cucumis sativus])

HSP 1 Score: 1043.9 bits (2698), Expect = 5.3e-301
Identity = 515/517 (99.61%), Postives = 516/517 (99.81%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINTS 60
           MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKL YTPINTS
Sbjct: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV
Sbjct: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300

Query: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360
           QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED
Sbjct: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360

Query: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420
           QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS
Sbjct: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420

Query: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480
           QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV
Sbjct: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480

Query: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRM+
Sbjct: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMV 517

BLAST of CSPI01G25160 vs. NCBI nr
Match: XP_008445709.1 (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo] >KAA0047097.1 cannabidiolic acid synthase-like [Cucumis melo var. makuwa] >TYK05061.1 cannabidiolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 991.9 bits (2563), Expect = 2.4e-285
Identity = 483/517 (93.42%), Postives = 502/517 (97.10%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINTS 60
           MKFSLLLPLSLAFI VCSSSSVWPSASSHVNHEEFL CLLHHSP SKSI KLVYTPINTS
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YS VLNFSIRNLRFS PNTPKPL IITP+NISHVQAAVICSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYHPF++VDLI+LSSVTIEV+QSTAWVQSGATLGELYYRIAEKSRTLAFPAGN PTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGFGTLLRKYGLAADNVIDAY+VDANG FHDRKSMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTICTISRTLEEEAIKL+DQWQYVANKLDEDLFLGINLLGGKI A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360
           QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED
Sbjct: 301 QGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIED 360

Query: 361 QPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMS 420
           QPLEVLLNRTPLA GNIKMKSDYVKEPI KATI+E+WQRL+SQDIEGANLVF+PYGGRMS
Sbjct: 361 QPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRMS 420

Query: 421 QISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYV 480
           QIS+SEIPFSHRAGNLYKIGYLTGWFEPG+NAEKTHLNWIRDIY YMTPFV+KSPRAAYV
Sbjct: 421 QISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYV 480

Query: 481 NYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           NYRDLDIGSNSKYGKTSYKRA VWG KYFG+NF+R++
Sbjct: 481 NYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLV 517

BLAST of CSPI01G25160 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 869.0 bits (2244), Expect = 2.3e-248
Identity = 422/518 (81.47%), Postives = 463/518 (89.38%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVW-PSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINT 60
           MKFS L+PLSLAFI +CSSSS+   ++SS++ +EEFLQCLLHHSP++ SI  LVY P N+
Sbjct: 1   MKFSPLIPLSLAFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 60

Query: 61  SYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEG 120
           SYSS+LNFSIRNLRFS P  PKPL IITP+NISH+QAAV+CSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPT 180
           LSYVAYHPFI+VDLI+LSSV ++V+QSTAWVQSGATLGELYYRIAEKSRTLAFPAG  PT
Sbjct: 121 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 180

Query: 181 VGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGG+GTLLRKYGLAADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKIS 300
           GIVVAWKVKLVPVPATVTICT +RT +E AIKL+DQWQYVANKLDEDLFL I L GGKIS
Sbjct: 241 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 300

Query: 301 AQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360
           AQG KINP ALFF+LFLGKADEL+ IL   FP+L L KE+CKETSWIES VYT NGLQI+
Sbjct: 301 AQGGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQID 360

Query: 361 DQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRM 420
           DQPLE  LNRTPLA G+ K+KSDYVKEPI KA IE IWQRL+SQDIEG NL  +PYGGRM
Sbjct: 361 DQPLEAFLNRTPLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPYGGRM 420

Query: 421 SQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 480
           SQIS+SEIPF HR GNLYKIGY+ GW E GV+AEK HLNWIR+IY YMTPFVSKSPRAAY
Sbjct: 421 SQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           VNYRDLDIGSN KYGKTSYKRA +WG KYFGNNFNR++
Sbjct: 481 VNYRDLDIGSNDKYGKTSYKRACIWGKKYFGNNFNRLV 518

BLAST of CSPI01G25160 vs. NCBI nr
Match: XP_011659211.2 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653319.1 hypothetical protein Csa_023346 [Cucumis sativus])

HSP 1 Score: 836.6 bits (2160), Expect = 1.3e-238
Identity = 403/522 (77.20%), Postives = 458/522 (87.74%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINTS 60
           MKFS L+PLSL F+ VCSSSS+W +ASS +NHEEF+QCLLHHSP +  I KLV+TPIN+S
Sbjct: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           +SS+LNF  RN RFS PNTPKPL IITP+NISH+QAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180
           SYVAYH FI+VDLI+L SVT++V+QSTAWV+SGATLGELYY+I +KSRTL FPAG  PTV
Sbjct: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKL+DQWQYVANKL+E+LFL I   GGK+SA
Sbjct: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300

Query: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRM 420
           DQPLE LLNRTP+  G+ K+KSDYVKEP+ KA I+ IW+RLESQDIEG  L  +PYGGRM
Sbjct: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420

Query: 421 SQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 480
           +QIS+S+IPF HRAG LY+IGY+ GW E GV AEK HLNWIR+IY YMTPFVSKSPRAAY
Sbjct: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMLAANS 522
           VNYRDLDIGSN+KYGK +YK+A V+G KYFGNNFNR++   S
Sbjct: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKS 522

BLAST of CSPI01G25160 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 823.9 bits (2127), Expect = 8.6e-235
Identity = 405/519 (78.03%), Postives = 451/519 (86.90%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVW-PSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINT 60
           MKFS L+ LSLAFI VCSSSS+   ++SS++ HEEFLQCLLHHSP++ SI KLVY PIN+
Sbjct: 1   MKFSPLILLSLAFIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINS 60

Query: 61  SYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEG 120
           SYSS+LNF  RN RFS PN P PL IITPTNISH+QAA+ICSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPT 180
           LSYVAY PFI+VDLI+L SVT++ ++S+AWVQSGATLGELYYRIAEKS+TLAFPAG  PT
Sbjct: 121 LSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGG+G LLRKYGLAADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGG+F
Sbjct: 181 VGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNF 240

Query: 241 GIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKIS 300
           G+VVAWKVKLVPVP TVTIC   RTLEE AI+L+DQWQYV NKL+EDLFL I L GGK S
Sbjct: 241 GVVVAWKVKLVPVPETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNS 300

Query: 301 AQ-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360
           AQ G K+NP ALFFSLFLGKADEL+AIL K FP+LGLTKE+CKE SWI S V   N  QI
Sbjct: 301 AQGGGKVNPTALFFSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQI 360

Query: 361 EDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
           EDQPLE LLNRTPLA G++K+KSDYVKE I K  IE IW+RL+SQDIEG  L  +PYGGR
Sbjct: 361 EDQPLEALLNRTPLANGSLKIKSDYVKELITKVGIEGIWERLKSQDIEGVTLALIPYGGR 420

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           M QISDSEIPFSHRAG  Y+IGY+ GW E G++AEK H+NWIR+IY YMTPFVSKSPRAA
Sbjct: 421 MGQISDSEIPFSHRAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           YVNYRD DIGSN+KYGK SYKRARVWGLKYFGNNFNR++
Sbjct: 481 YVNYRDHDIGSNNKYGKVSYKRARVWGLKYFGNNFNRLV 519

BLAST of CSPI01G25160 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 503.4 bits (1295), Expect = 2.4e-142
Identity = 247/519 (47.59%), Postives = 356/519 (68.59%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCS--SSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPIN 60
           MKF       L F    +   S   P +++  N   FLQCL      S  ++K+++TP +
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  TSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFE 120
           TS+SSVL  SI+N RFS P+ PKP+ I+TP   S VQ+AV C++  G+ IR RSGGHD+E
Sbjct: 61  TSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYE 120

Query: 121 GLSYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSP 180
           GLSYV + PF+I+DL +L S+T++V   + WVQ+GAT+GELYY I +K+RTLAFPAG  P
Sbjct: 121 GLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCP 180

Query: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGS 240
           TVGVGGHFSGGG+GTLLRK+GLAAD+VIDA +VDA G   +R+ MGED FWAIRGGGG S
Sbjct: 181 TVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSS 240

Query: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKI 300
           F +V++WK+ L+ VP+TVT+  +++  E+ A+K++ +WQ+VA+K+ +DLF+       ++
Sbjct: 241 FCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFI-------RV 300

Query: 301 SAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQI 360
             Q  K    A F  L+LG    L+ ++NK FP+LGL +++C E SWIESV++     ++
Sbjct: 301 MLQRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---EL 360

Query: 361 EDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGR 420
            ++P+ VL  RT  A+   K KSD+V+EP+PK  I ++W+RL+  + E A L+F P+GG+
Sbjct: 361 GEEPINVLTKRT-RASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGK 420

Query: 421 MSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAA 480
           MS+I+D E PF HR GN+Y+I YL  W     + ++ ++ W+  +Y  M+ FV+KSPR A
Sbjct: 421 MSEIADYETPFPHRKGNIYEIQYLNYW---RGDVKEKYMRWVERVYDDMSEFVAKSPRGA 480

Query: 481 YVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           Y+N RDLD+G      ++ Y+  + WG+KYF NNF R++
Sbjct: 481 YINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLV 505

BLAST of CSPI01G25160 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 502.3 bits (1292), Expect = 5.3e-142
Identity = 250/519 (48.17%), Postives = 357/519 (68.79%), Query Frame = 0

Query: 6   LLPLSLAFIAVCSSSSVWPSASS-HVNHEEFLQCLLHHSPHSKSIAKLVYTPINTSYSSV 65
           LL L + F+ +  S S +PS S+   NHE FL+CL H      S  ++++T  + SY S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSI 66

Query: 66  LNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVA 125
           LN SI+N RF +  TPKP+ IITP   + VQ+ + C++ HG+ IR RSGGHD+EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 -YHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVG 185
              PF+++DL +L S+T++V   T WVQSGAT+GELYY I + S++LAFPAG  PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVV 245
           G F GGG+GTL+RKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGD 305
           +WK++L+ VP+ VT+  + +T E+EA+ ++++WQY+A+K+  DLF+   L          
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML--------QK 306

Query: 306 KINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPL 365
           +    A F  L+LG   +L+A++   FP+LGL    C+E SWIESV++      I+ + +
Sbjct: 307 ETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWF-----IKGESM 366

Query: 366 EVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQIS 425
           E+L  R    + + K K D+++EPIPK  I+ +W+R E+ +   A ++  P+GG+MS+I+
Sbjct: 367 EILAKR-KRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIA 426

Query: 426 DSEIPFSHRAGNLYKIGYLTGWFEP----GVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 485
           D+EIPF HR GNLY+I YL  W E       N EK +L W+  +Y +MTP+VSKSPR AY
Sbjct: 427 DNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEK-YLRWVESVYEFMTPYVSKSPRRAY 486

Query: 486 VNYRDLDIGSNSKYG-KTSYKRARVWGLKYFGNNFNRML 518
           VN+RD+D+G       KT Y+ A+VWG+KYF NNF+R++
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLV 508

BLAST of CSPI01G25160 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 498.8 bits (1283), Expect = 5.9e-141
Identity = 244/513 (47.56%), Postives = 358/513 (69.79%), Query Frame = 0

Query: 10  SLAFIAVCSSSSVWPSASSH-VNHEEFLQCLLHHSPHSKSIAKLVYTPINTSYSSVLNFS 69
           S  F  +  S ++ P  S+H  NHE+FL+CL +    +    K+++T  ++S+ S+L+ S
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 70  IRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYH-P 129
           I+N RFS+  TPKP+ IITP   S VQ  + C++ HG+ +R RS GH +EGLSY+AY+ P
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 130 FIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFS 189
           F ++DL +L S++++V   T WVQ+GAT GELYY I + +++LAFPAG  PTVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 190 GGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKV 249
           GGG+GTLLRKYGLAADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 250 KLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGDKINP 309
           KLV VP+T+T+  + +T ++EA++++ +WQY A+K+ +DLF+   L       + +K   
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL------ERSNKNAV 304

Query: 310 IALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLL 369
            ALF  L++G  + L+A++ + FP+LGL KE C+E SWIESV++  +  + E   L VL 
Sbjct: 305 HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGES--LGVLT 364

Query: 370 NRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRMSQISDSEI 429
           NR   +  + K K D+V+EPIP+A I+EIW+RLE+ +     ++  P+GG+MS++++ E 
Sbjct: 365 NRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 424

Query: 430 PFSHRAGNLYKIGYLTGW-FEPGVNAEKT--HLNWIRDIYGYMTPFVSKSPRAAYVNYRD 489
           PF HR GNLY+I Y+  W  E   N  +T  +L W+  +Y +MTP+VSKSPR AYVN++D
Sbjct: 425 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 490 LDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           +D+G      KT Y+  + WG+KYF NNF R++
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLV 508

BLAST of CSPI01G25160 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 484.6 bits (1246), Expect = 1.1e-136
Identity = 253/522 (48.47%), Postives = 347/522 (66.48%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPINTS 60
           MK++L+L L   F  V     +W S+SS  N E F QCL  +S     I+  ++   N S
Sbjct: 1   MKYALILVL---FFVV----FIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGS 60

Query: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120
           YSSVL  +IRNLRF+  +TPKP  II  T+ SHVQAA+ C K H LQ++IRSGGHD++GL
Sbjct: 61  YSSVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGL 120

Query: 121 SYVAY--HPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSP 180
           SYV Y   PF ++D+ +L SV ++V   TAWVQ+GA LGE+YY I EKS+TLA+PAG  P
Sbjct: 121 SYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICP 180

Query: 181 TVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGS 240
           TVGVGGH SGGG+G ++RKYGL  DN IDA +VD NG   DRK MGEDL+WAI GGGGGS
Sbjct: 181 TVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGS 240

Query: 241 FGIVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLG--INLLGG 300
           +G+V+A+K+ LV VP  VT+  ISRTLE+ A  ++ +WQ VA KL ++LF+   I+++ G
Sbjct: 241 YGVVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNG 300

Query: 301 KISAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGL 360
            +S+Q         F ++FLG    L++ILN+ FP+LGL + +C ETSWI+SV++  N  
Sbjct: 301 TVSSQK---TVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN-- 360

Query: 361 QIEDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYG 420
            I+    E LL +       +K KSDYV+EPI +  +E IW+++   ++E   + F PYG
Sbjct: 361 -IQVGSSETLLLQRNQPVNYLKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPYG 420

Query: 421 GRMSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPR 480
           G M +IS +  PF +RAGNL+KI Y   W +  +     ++   R +Y +MTPFVSK+PR
Sbjct: 421 GEMGRISSTVTPFPYRAGNLWKIQYGANWRDETLT--DRYMELTRKLYQFMTPFVSKNPR 480

Query: 481 AAYVNYRDLDIGSNSKYGK-TSYKRARVWGLKYFGNNFNRML 518
            ++ NYRD+D+G NS  GK +SY   + +G KYF  NF R++
Sbjct: 481 QSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLV 505

BLAST of CSPI01G25160 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 480.7 bits (1236), Expect = 1.7e-135
Identity = 240/522 (45.98%), Postives = 352/522 (67.43%), Query Frame = 0

Query: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLVYTPIN-- 60
           M  S  LPL    +   S  ++ P++S     ++F+ C+  ++     + K  + P    
Sbjct: 1   MGISKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNV 60

Query: 61  TSYSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFE 120
           + +S VL  + +NLRF   + PKP FI +P + SHVQA++ICSK   + +R+RSGGHD+E
Sbjct: 61  SMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYE 120

Query: 121 GLSYVAY--HPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGN 180
           GLSYV+    PFI++DL  +  V I ++ ++AWVQSGAT+GELYYRIAEKS+   FPAG 
Sbjct: 121 GLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGL 180

Query: 181 SPTVGVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGG 240
             ++G+GGH +GG +G+++RKYGL ADNV+DA +VDANG   DR +MGED FWAIRGG G
Sbjct: 181 CSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAG 240

Query: 241 GSFGIVVAWKVKLVPVPATVTICTISRTLEEE-AIKLVDQWQYVANKLDEDLFLGINLLG 300
           GSFGI++AWK+KLVPVP TVT+ T+++TL+++   K++ +WQ VA+KL E+LF+ +    
Sbjct: 241 GSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF-- 300

Query: 301 GKISAQGDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNG 360
             ++  G        + +LFLG    LM ++ K+FP+LGLT ++C E SW+ES+ Y  +G
Sbjct: 301 -NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYI-SG 360

Query: 361 LQIEDQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPY 420
                    +L  ++P    + K KSD+VK PIP++ ++ I+++L  +DI    +++ PY
Sbjct: 361 FPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPY 420

Query: 421 GGRMSQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSP 480
           GG M++I +S+IPF HR G L+K+ Y+T W +      + H+NWIRD+Y YMTP+VS +P
Sbjct: 421 GGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSR-HINWIRDLYSYMTPYVSSNP 480

Query: 481 RAAYVNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRML 518
           R AYVNYRDLD+G N+K  KT  K+A+VWG  YF NNFNR++
Sbjct: 481 REAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLM 515