CSPI01G25150 (gene) Cucumber (PI 183967) v1

BLAST of CSPI01G25150 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 6.6e-152
Identity = 268/533 (50.28%), Postives = 373/533 (69.98%), Query Frame = 0

Query: 5   SQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSSYSSV 64
           S ++  F   T++LS  P  VSA      +F+QCLS   + S  +SKV+HTP ++S+SSV
Sbjct: 8   SSVLIFFTTSTLLLSI-PHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 65  LNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLSYVA 124
           L  SI+N RFS P+ PKP+LI+TP   S +Q+AV C++   + IRTRSGGHDYEGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 125 YHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGG 184
           + PF+I+DL NLRSI++D+ N + WV++GAT+GELYY IG+K+RTLAFPAG+CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 185 HFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFGIVVA 244
           HFSGGGYG LLRK+GLAAD+VIDA +VDA G +L+R  MGED FWAIRGG G SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 245 WKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGI 304
           WK+ L+ VP+ VT+ +  K  E  A+K+IH+WQ+VA+K+ +D+F+ ++L           
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQ--------RY 307

Query: 305 KNPI-ATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTIESL 364
           KN + A+F  L+LG V  L+  ++ +FPELGL + DC E+ W+ES +  A       E +
Sbjct: 308 KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAE---LGEEPI 367

Query: 365 EPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQIS 424
             L  RT  +L + K KSD++++P+PK AI  +W+RL+  + E  Q +F P+G +MS+I+
Sbjct: 368 NVLTKRTRASL-AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 427

Query: 425 KSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYR 484
             ETPF HR G +Y+I Y+  W+G     K +++ W+  +Y+ M+ FV+KSPR AY N R
Sbjct: 428 DYETPFPHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINLR 487

Query: 485 DLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 537
           DLD+G      ++ Y++   WG+KYF NNF+RLV VKT VDP DFF  EQSIP
Sbjct: 488 DLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G25150 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 8.7e-152
Identity = 270/532 (50.75%), Postives = 374/532 (70.30%), Query Frame = 0

Query: 10  LFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSI 69
           LF +L   L+ SP  VSA    H++F++CLS   + +    KV+HT  +SS+ S+L+ SI
Sbjct: 7   LFTILLFSLNISP-LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSI 66

Query: 70  RNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLSYVAYH-PF 129
           +N RFS  ETPKP+ IITP   S +Q  + C++ H + +RTRS GH YEGLSY+AY+ PF
Sbjct: 67  QNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPF 126

Query: 130 IIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSG 189
            ++DL NLRSIS+D+ N T WV++GAT GELYY IG+ +++LAFPAGI PTVGVGG FSG
Sbjct: 127 AVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSG 186

Query: 190 GGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFGIVVAWKVK 249
           GGYG LLRKYGLAADN+IDA +VDA+G +LDR++MGED FWAIRGG G SFG++++WKVK
Sbjct: 187 GGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVK 246

Query: 250 LVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKNPI 309
           LV VP+ +T+    K  + +AV++I +WQY A+K+ +D+F+   L   N       KN +
Sbjct: 247 LVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN-------KNAV 306

Query: 310 -ATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTIESLEPLL 369
            A F  L++G V+ L+A +  KFPELGL K+ C E+ W+ES L  A   F   ESL  L 
Sbjct: 307 HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FPKGESLGVLT 366

Query: 370 NRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISKSET 429
           NR  T+L S K K D++++PIP+AAI+ IW+RL+A +    + +  P+G +MS++++ ET
Sbjct: 367 NRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 426

Query: 430 PFSHRAGYLYQIGYMVGWKGQNLKAK---NRHISWIRELYEYMAPFVSKSPRAAYANYRD 489
           PF HR G LY+I Y+  W+ +  K K   ++++ W+  +YE+M P+VSKSPR AY N++D
Sbjct: 427 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 486

Query: 490 LDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 537
           +D+G      KT Y++   WG+KYF NNF+RLV VKT+VDP DFF  EQSIP
Sbjct: 487 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of CSPI01G25150 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 8.1e-150
Identity = 266/512 (51.95%), Postives = 358/512 (69.92%), Query Frame = 0

Query: 33  QNFVQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSIRNLRFSKPETPKPLLIITPSHVS 92
           +NF++C S++   +   +K+V+T  +  Y S+LN +I+NLRF+   TPKPL+IITP +VS
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HIQAAVICSKSHDLQIRTRSGGHDYEGLSYVAYHPFIIVDLINLRSISIDIVNNTAWVES 152
           HIQ  ++CSK   LQIRTRSGGHD EG+SY++  PF+IVDL N+ S+ ID+ + TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGMVLDRESMGEDLFWAIRGGSGGSFGIVVAWKVKLVPVPAIVTICSTIKNLE-DDAVK 272
            +G VLDR+SMGEDLFWAIRGG G +FGI+ AWK++LV VP++ TI S  KN+E  + VK
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 VIHQWQYVANKLHEDIFLGI-VLTGGNTSTQGGIKNPIATFY-SLFLGKVDELVATLSTK 332
           ++++WQ +A    +++ L    +T   T  QG  K  I +++ S+F G VD LV  ++  
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLIKQDCLEVRWVESTLIIASGVFQ---TIESLEPLLNRTPTTLDSTKIKSDYIKK 392
           FPELG+ K DC ++ W++ T+I  SGV     T    E LL+R+     +  IK DY+KK
Sbjct: 332 FPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 393 PIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISKSETPFSHRAGYLYQIGYMVGWK 452
           PIP+ A+  I ++L   D+    FVF PYG  M +IS+S  PF HRAG +Y+I Y+  W+
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWE 451

Query: 453 GQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGSNNKYGKTSYKQASIWGL 512
            Q  +   +HI+WIR +Y +  P+VS++PR AY NYRDLD+G  N     +Y QA IWG 
Sbjct: 452 KQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 513 KYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           KYFG NF RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G25150 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.4e-149
Identity = 266/512 (51.95%), Postives = 357/512 (69.73%), Query Frame = 0

Query: 33  QNFVQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSIRNLRFSKPETPKPLLIITPSHVS 92
           +NF++CLS++   +   +K+V+T  +  Y S+LN +++NLRF+   TPKPL+I TP +VS
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 93  HIQAAVICSKSHDLQIRTRSGGHDYEGLSYVAYHPFIIVDLINLRSISIDIVNNTAWVES 152
           HIQ  ++CSK   LQIRTRSGGHD EG+SY++  PF+IVDL N+ S+ ID+ + TAWVES
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 153 GATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGMVLDRESMGEDLFWAIRGGSGGSFGIVVAWKVKLVPVPAIVTICSTIKNLE-DDAVK 272
            +G VLDR+SMGEDLFWAIRGG G +FGI+ AWK++LV VP++ TI S  KN+E  + VK
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 VIHQWQYVANKLHEDIFLGI-VLTGGNTSTQGGIKNPIATFY-SLFLGKVDELVATLSTK 332
           ++++WQ +A    +++ L    +T   T  QG  K  I +++ S+F G VD LV  ++  
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLIKQDCLEVRWVESTLIIASGVFQ---TIESLEPLLNRTPTTLDSTKIKSDYIKK 392
           FPELG+ K DC ++ W++ T+I  SGV     T    E LL+R+     +  IK DY+KK
Sbjct: 332 FPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 393 PIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISKSETPFSHRAGYLYQIGYMVGWK 452
           PIP+ A+  I ++L   D+    FVF PYG  M +IS+S  PF HRAG  Y+I Y+  W+
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWE 451

Query: 453 GQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGSNNKYGKTSYKQASIWGL 512
            Q  +   +HI+WIR +Y +  P+VS++PR AY NYRDLD+G  N     +Y QA IWG 
Sbjct: 452 KQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 513 KYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           KYFG NF RLV VKTKVDP +FFR+EQSIP L
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G25150 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 4.0e-149
Identity = 265/511 (51.86%), Postives = 351/511 (68.69%), Query Frame = 0

Query: 33  QNFVQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSIRNLRFSKPETPKPLLIITPSHVS 92
           +NF++C S+H   +    K+V+T  +  Y S+LN +I+NLRF    TPKPL+I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 93  HIQAAVICSKSHDLQIRTRSGGHDYEGLSYVAYHPFIIVDLINLRSISIDIVNNTAWVES 152
           HIQA ++CSK   LQIRTRSGGHD EG+SY++  PF++VDL N+ SI ID+ + TAWVE+
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 153 GATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVD 212
           GATLGE+YY I EK+  L+FP G CPTVGVGGHFSGGGYG L+R YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGMVLDRESMGEDLFWAIRGGSGGSFGIVVAWKVKLVPVPAIVTICSTIKNLE-DDAVK 272
            +G VLDR+SMGEDLFWAIRGG G +FGI+ AWK+KLV VP+  TI S  KN+E    VK
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 273 VIHQWQYVANKLHEDIFLGI-VLTGGNTSTQGGIKNPI-ATFYSLFLGKVDELVATLSTK 332
           + ++WQ +A K  +D+ L    +T   T   G  K  +   F S+F G VD LV  ++  
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLIKQDCLEVRWVESTLIIASGV-FQTIE-SLEPLLNRTPTTLDSTKIKSDYIKKP 392
           FPELG+ K DC E  W+++T+  +  V F T     E LL+R+     +  IK DY+KKP
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 393 IPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISKSETPFSHRAGYLYQIGYMVGWKG 452
           IP+ A+  I ++L   D+    +V  PYG  M +IS+S  PF HRAG +Y++ Y   W+ 
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

Query: 453 QNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYRDLDIGSNNKYGKTSYKQASIWGLK 512
           Q  +   +HI+W+R +Y +  P+VS++PR AY NYRDLD+G  N     +Y QA IWG K
Sbjct: 452 Q--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511

Query: 513 YFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           YFG NF RLV VKTKVDP++FFR+EQSIP L
Sbjct: 512 YFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CSPI01G25150 vs. ExPASy TrEMBL
Match: A0A1S3BDB7 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488652 PE=3 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 5.8e-292
Identity = 496/539 (92.02%), Postives = 512/539 (94.99%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTI-VLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINS 60
           MKSSSQL PLF VL I V+S+SP WVSAFPH+HQNF+ CL EHSSKSYPISKVV+TPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEG 120
           SYSSVLNFSIRN RFSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHDYEG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180
           LSYVAY PFIIVDLIN+RSIS+DIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSF 240
           VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGM  DRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300
           GIVVAWKVKLVPVPA+VTICSTIKNLE DAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQGGIK P ATFYSLFLGKVDELVATLST FPE GLIK+DCLEVRW+ESTLIIASGVFQT
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 IESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           +ESL+PLLNRTP+T+DSTKIKSDYIKKPIPKAAIEGIWQRLKA DIE PQ VF PYGARM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQIS+SETPFSHRAGYLY+IGYMVGWKGQNLKAKNRHISW RELYEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
            NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVK KVDPHDFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of CSPI01G25150 vs. ExPASy TrEMBL
Match: A0A5D3C1B2 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00230 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.8e-291
Identity = 495/539 (91.84%), Postives = 511/539 (94.81%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTI-VLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINS 60
           MKSSSQL PLF VL I V+S+SP WVSAFPH+HQNF+ CL EHSSKSYPISKVV+TPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEG 120
           SYSSVLNFSIRN RFSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHDYEG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180
           LSYVAY PFIIVDLIN+RSIS+DIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSF 240
           VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGM  DRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300
           GIVVAWKVKLVPVPA+VTICSTIKNLE DAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQGGIK P ATFYSLFLGKVDELVATLST FPE GLIK+DCLEVRW+ESTLIIASGVFQT
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 IESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           +ESL+PLLNRTP+T+DSTKIKSDYIKKPIPKAA EGIWQRLKA DIE PQ VF PYGARM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAANEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQIS+SETPFSHRAGYLY+IGYMVGWKGQNLKAKNRHISW RELYEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
            NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVK KVDPHDFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of CSPI01G25150 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.1e-258
Identity = 442/538 (82.16%), Postives = 484/538 (89.96%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSS 60
           MK SS LIPL LV  +V SAS    SA P K+QNF+QCLSEHSSKSYPISKVV+TPINSS
Sbjct: 1   MKFSSTLIPLSLVFIVVSSAS-LCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120
           YSSVL+FSIRNL FSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHD+EGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180
           SYVAY PFI+VDLINLRS+ +++ NNTAWVESGATLGELYYRIGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFG 240
           G+GGHFSGGGYG +LRK+GLAADNVIDAYLVDANG VLD+ESMGEDLFWAIRGG GGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300
           IVVAWK+KLVPVP  VTICST +NLE+DA+K+IH+WQYV NKL E+I+LGI+LTGGN ST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTI 360
           Q GI NP A F+SLFLG+ DELV TLST FPELGLIKQDC+E  WVESTLII  GV Q I
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGV-QPI 360

Query: 361 ESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMS 420
           E+LEPLLNRT TTLDSTKIKSDYI +PIP AAI+ IWQRLK +DIE  Q VF PYG RMS
Sbjct: 361 ETLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMS 420

Query: 421 QISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYA 480
           QIS+SETPFSHRA YL++IGY+VGWK Q+LKAK RHISW+RE+YEYMAPFVSKSPRAAY 
Sbjct: 421 QISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYT 480

Query: 481 NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           NYRDLDIGSNNKYGKTSYK+AS+WGLKYFGNNF RLVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 NYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of CSPI01G25150 vs. ExPASy TrEMBL
Match: A0A1S3BDA8 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.1e-258
Identity = 442/538 (82.16%), Postives = 484/538 (89.96%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSS 60
           MK SS LIPL LV  +V SAS    SA P K+QNF+QCLSEHSSKSYPISKVV+TPINSS
Sbjct: 1   MKFSSTLIPLSLVFIVVSSAS-LCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120
           YSSVL+FSIRNL FSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHD+EGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180
           SYVAY PFI+VDLINLRS+ +++ NNTAWVESGATLGELYYRIGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFG 240
           G+GGHFSGGGYG +LRK+GLAADNVIDAYLVDANG VLD+ESMGEDLFWAIRGG GGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300
           IVVAWK+KLVPVP  VTICST +NLE+DA+K+IH+WQYV NKL E+I+LGI+LTGGN ST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTI 360
           Q GI NP A F+SLFLG+ DELV TLST FPELGLIKQDC+E  WVESTLII  GV Q I
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGV-QPI 360

Query: 361 ESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMS 420
           E+LEPLLNRT TTLDSTKIKSDYI +PIP AAI+ IWQRLK +DIE  Q VF PYG RMS
Sbjct: 361 ETLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMS 420

Query: 421 QISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYA 480
           QIS+SETPFSHRA YL++IGY+VGWK Q+LKAK RHISW+RE+YEYMAPFVSKSPRAAY 
Sbjct: 421 QISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYT 480

Query: 481 NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           NYRDLDIGSNNKYGKTSYK+AS+WGLKYFGNNF RLVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 NYRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of CSPI01G25150 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.3e-255
Identity = 435/539 (80.71%), Postives = 482/539 (89.42%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSS 60
           MK SS LIP  L+L I++S+S SWVSA PHKHQNF+QCLSEHSSK YPISKVVHTPINSS
Sbjct: 1   MKYSSPLIPFSLIL-ILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120
           YSSVL+FSIRNL FSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHD+EGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180
           SYVAYHPFIIVDLINLRSI ID+  NTAWVESGATLGELYYRIGEKSRTLAFP G+CPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFG 240
           GVGGHFSGGGYG +LRK+GLAADNVIDAYLVDANG V DR+SMGEDLFWAIRGG GGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGN-TS 300
           IVVAWK+KLV VPA VTICST +NL+ D +K++HQWQYVANKL E++FLGI+LTGGN T+
Sbjct: 241 IVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITT 300

Query: 301 TQGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQ GI NPIATF+SLFLGKVD LVATLS  FPELG  K+DC+E  W+ESTLI A+GV  T
Sbjct: 301 TQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGV-GT 360

Query: 361 IESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           +ESLEPLLNRTP+ L++ K+KSDY K+PI  A IEG+WQRLKA+DIE  Q +F PYG RM
Sbjct: 361 VESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRM 420

Query: 421 SQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQIS+ ETPFSHRAG LY+IGY V WK Q+LKAK +HISWIR++Y+YM PFVSKSPR+AY
Sbjct: 421 SQISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           ANYRDLDIG+N KYGKTS +QASIWGLKYFGNNFKRLVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 ANYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of CSPI01G25150 vs. NCBI nr
Match: XP_011659198.2 (berberine bridge enzyme-like 18 [Cucumis sativus])

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 535/542 (98.71%), Postives = 537/542 (99.08%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSS 60
           MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVV+TPINSS
Sbjct: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120
           YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL
Sbjct: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180
           SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV
Sbjct: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFG 240
           GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMV DRESMGEDLFWAIRGGSGGSFG
Sbjct: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFG 240

Query: 241 IVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300
           IVVAWKVKLVPVPA+VTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTST
Sbjct: 241 IVVAWKVKLVPVPAMVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300

Query: 301 QGG----IKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGV 360
           QGG    IKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGV
Sbjct: 301 QGGIKNPIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGV 360

Query: 361 FQTIESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYG 420
           FQTIESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYG
Sbjct: 361 FQTIESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYG 420

Query: 421 ARMSQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPR 480
           ARMSQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPR
Sbjct: 421 ARMSQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPR 480

Query: 481 AAYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 539
           AAYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP
Sbjct: 481 AAYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 540

BLAST of CSPI01G25150 vs. NCBI nr
Match: XP_008445695.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.2e-291
Identity = 496/539 (92.02%), Postives = 512/539 (94.99%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTI-VLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINS 60
           MKSSSQL PLF VL I V+S+SP WVSAFPH+HQNF+ CL EHSSKSYPISKVV+TPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEG 120
           SYSSVLNFSIRN RFSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHDYEG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180
           LSYVAY PFIIVDLIN+RSIS+DIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSF 240
           VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGM  DRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300
           GIVVAWKVKLVPVPA+VTICSTIKNLE DAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQGGIK P ATFYSLFLGKVDELVATLST FPE GLIK+DCLEVRW+ESTLIIASGVFQT
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 IESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           +ESL+PLLNRTP+T+DSTKIKSDYIKKPIPKAAIEGIWQRLKA DIE PQ VF PYGARM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQIS+SETPFSHRAGYLY+IGYMVGWKGQNLKAKNRHISW RELYEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
            NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVK KVDPHDFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of CSPI01G25150 vs. NCBI nr
Match: TYK05058.1 (tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1010.0 bits (2610), Expect = 7.7e-291
Identity = 495/539 (91.84%), Postives = 511/539 (94.81%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTI-VLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINS 60
           MKSSSQL PLF VL I V+S+SP WVSAFPH+HQNF+ CL EHSSKSYPISKVV+TPINS
Sbjct: 1   MKSSSQLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINS 60

Query: 61  SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEG 120
           SYSSVLNFSIRN RFSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHDYEG
Sbjct: 61  SYSSVLNFSIRNFRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120

Query: 121 LSYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180
           LSYVAY PFIIVDLIN+RSIS+DIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT
Sbjct: 121 LSYVAYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 180

Query: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSF 240
           VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGM  DRESMGEDLFWAIRGG GGSF
Sbjct: 181 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300
           GIVVAWKVKLVPVPA+VTICSTIKNLE DAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS
Sbjct: 241 GIVVAWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTS 300

Query: 301 TQGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQGGIK P ATFYSLFLGKVDELVATLST FPE GLIK+DCLEVRW+ESTLIIASGVFQT
Sbjct: 301 TQGGIKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQT 360

Query: 361 IESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           +ESL+PLLNRTP+T+DSTKIKSDYIKKPIPKAA EGIWQRLKA DIE PQ VF PYGARM
Sbjct: 361 VESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAANEGIWQRLKAGDIEAPQLVFVPYGARM 420

Query: 421 SQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQIS+SETPFSHRAGYLY+IGYMVGWKGQNLKAKNRHISW RELYEYM PFVSKSPR AY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
            NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVK KVDPHDFFRHEQSIPTL
Sbjct: 481 VNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of CSPI01G25150 vs. NCBI nr
Match: XP_004151192.3 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical protein Csa_023212 [Cucumis sativus])

HSP 1 Score: 909.4 bits (2349), Expect = 1.4e-260
Identity = 445/539 (82.56%), Postives = 489/539 (90.72%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSS 60
           MK SS LIP  L+L IV+S+S  WVSA PHKH NF+QCLSEHSSKSYPISKVVHTPINSS
Sbjct: 1   MKYSSPLIPFSLIL-IVVSSSSLWVSASPHKHHNFLQCLSEHSSKSYPISKVVHTPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120
           YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHDYEGL
Sbjct: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180
           SYVA HPFII+DLINLRS+ ID+ +NTAWVESG TLGELYYRIGEKSRTLAFPAGICPTV
Sbjct: 121 SYVASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFG 240
           GVGGHFSGGGYG +LRK+GLAADNVIDAYLVDANG V DRESMGEDLFWAIRGG GGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGN-TS 300
           IVVAWK+KLV VPA VT+C+T +NL+ D +K++HQWQYVANKL E++FLGI+LTGGN T+
Sbjct: 241 IVVAWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITT 300

Query: 301 TQGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQT 360
           TQ GI NPIATF+SLFLGKV+ELVATLST FPELGLIKQDC+E  W+ESTLI ++GV QT
Sbjct: 301 TQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGV-QT 360

Query: 361 IESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARM 420
           +ESLEPLLNRTP+ L++ KIKSDYIK+PI  A IEGIWQRLKA+DIE  Q +F PYG RM
Sbjct: 361 VESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRM 420

Query: 421 SQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAY 480
           SQIS+SETPFSHR G LY+IGY++GWK Q+LKAK +HISWIRE+YEYM PFVSKSPR+AY
Sbjct: 421 SQISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAY 480

Query: 481 ANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           ANYRDLDIG N KYGKTS KQASIWGLKYFGNNFKRLVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 ANYRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of CSPI01G25150 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 906.0 bits (2340), Expect = 1.6e-259
Identity = 438/538 (81.41%), Postives = 494/538 (91.82%), Query Frame = 0

Query: 1   MKSSSQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSS 60
           MK SS LIPL LVL IVL + P W +A P K+Q+F+QCLSEHSS SYPISKVV++PINSS
Sbjct: 1   MKFSSPLIPLALVL-IVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSS 60

Query: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGL 120
           YSSVL+FSI+NLRFSKP+TPKPLLIITPSHVSHIQAAVICSKSH LQIRTRSGGHD+EGL
Sbjct: 61  YSSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVAYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTV 180
           SYVAY PFI+VDLINLRSIS+D  NNTAWVESGATLGELYYRIGEKSRTLAFPAG+CPTV
Sbjct: 121 SYVAYRPFIVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFG 240
           G+GGHFSGGGYG +LRK+GLAADNVIDA+LVDANG V DRESMGEDLFWAIRGG GGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTST 300
           IVVAWK+KLVPVPA VTICST +NLE+DA+K++H+WQYVANKL E+++LGI+LTGG  ST
Sbjct: 241 IVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKAST 300

Query: 301 QGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTI 360
           QGGI NP ATF+SLFLG++DEL+ATLST FPELGLIKQDC+E  WVESTLIIA+GV Q I
Sbjct: 301 QGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGV-QPI 360

Query: 361 ESLEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMS 420
           ESLEPLLNRTP T+++TKIKSDY+K+P+P+AAIEGI QRLKA+DIE  Q +F PYG +MS
Sbjct: 361 ESLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMS 420

Query: 421 QISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYA 480
           QIS+SETPFSHRAG LY+IGY+VGWK Q+LKAK RHI WIRE+YEYM PFVSKSPRAAYA
Sbjct: 421 QISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYA 480

Query: 481 NYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           NYRDLDIG+NNKYG+TS +QAS+WGLKYFGNNFK+LVYVKTKVDP+DFFRHEQSIPTL
Sbjct: 481 NYRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of CSPI01G25150 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 538.9 bits (1387), Expect = 4.7e-153
Identity = 268/533 (50.28%), Postives = 373/533 (69.98%), Query Frame = 0

Query: 5   SQLIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSSYSSV 64
           S ++  F   T++LS  P  VSA      +F+QCLS   + S  +SKV+HTP ++S+SSV
Sbjct: 8   SSVLIFFTTSTLLLSI-PHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 67

Query: 65  LNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLSYVA 124
           L  SI+N RFS P+ PKP+LI+TP   S +Q+AV C++   + IRTRSGGHDYEGLSYV 
Sbjct: 68  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 127

Query: 125 YHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGG 184
           + PF+I+DL NLRSI++D+ N + WV++GAT+GELYY IG+K+RTLAFPAG+CPTVGVGG
Sbjct: 128 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 187

Query: 185 HFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFGIVVA 244
           HFSGGGYG LLRK+GLAAD+VIDA +VDA G +L+R  MGED FWAIRGG G SF +V++
Sbjct: 188 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 247

Query: 245 WKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGI 304
           WK+ L+ VP+ VT+ +  K  E  A+K+IH+WQ+VA+K+ +D+F+ ++L           
Sbjct: 248 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQ--------RY 307

Query: 305 KNPI-ATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTIESL 364
           KN + A+F  L+LG V  L+  ++ +FPELGL + DC E+ W+ES +  A       E +
Sbjct: 308 KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAE---LGEEPI 367

Query: 365 EPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQIS 424
             L  RT  +L + K KSD++++P+PK AI  +W+RL+  + E  Q +F P+G +MS+I+
Sbjct: 368 NVLTKRTRASL-AFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 427

Query: 425 KSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANYR 484
             ETPF HR G +Y+I Y+  W+G     K +++ W+  +Y+ M+ FV+KSPR AY N R
Sbjct: 428 DYETPFPHRKGNIYEIQYLNYWRGD---VKEKYMRWVERVYDDMSEFVAKSPRGAYINLR 487

Query: 485 DLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 537
           DLD+G      ++ Y++   WG+KYF NNF+RLV VKT VDP DFF  EQSIP
Sbjct: 488 DLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G25150 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 538.5 bits (1386), Expect = 6.2e-153
Identity = 270/532 (50.75%), Postives = 374/532 (70.30%), Query Frame = 0

Query: 10  LFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSI 69
           LF +L   L+ SP  VSA    H++F++CLS   + +    KV+HT  +SS+ S+L+ SI
Sbjct: 7   LFTILLFSLNISP-LVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSI 66

Query: 70  RNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLSYVAYH-PF 129
           +N RFS  ETPKP+ IITP   S +Q  + C++ H + +RTRS GH YEGLSY+AY+ PF
Sbjct: 67  QNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPF 126

Query: 130 IIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSG 189
            ++DL NLRSIS+D+ N T WV++GAT GELYY IG+ +++LAFPAGI PTVGVGG FSG
Sbjct: 127 AVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSG 186

Query: 190 GGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFGIVVAWKVK 249
           GGYG LLRKYGLAADN+IDA +VDA+G +LDR++MGED FWAIRGG G SFG++++WKVK
Sbjct: 187 GGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVK 246

Query: 250 LVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKNPI 309
           LV VP+ +T+    K  + +AV++I +WQY A+K+ +D+F+   L   N       KN +
Sbjct: 247 LVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN-------KNAV 306

Query: 310 -ATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTIESLEPLL 369
            A F  L++G V+ L+A +  KFPELGL K+ C E+ W+ES L  A   F   ESL  L 
Sbjct: 307 HALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FPKGESLGVLT 366

Query: 370 NRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISKSET 429
           NR  T+L S K K D++++PIP+AAI+ IW+RL+A +    + +  P+G +MS++++ ET
Sbjct: 367 NRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 426

Query: 430 PFSHRAGYLYQIGYMVGWKGQNLKAK---NRHISWIRELYEYMAPFVSKSPRAAYANYRD 489
           PF HR G LY+I Y+  W+ +  K K   ++++ W+  +YE+M P+VSKSPR AY N++D
Sbjct: 427 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 486

Query: 490 LDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIP 537
           +D+G      KT Y++   WG+KYF NNF+RLV VKT+VDP DFF  EQSIP
Sbjct: 487 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of CSPI01G25150 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.1e-149
Identity = 263/533 (49.34%), Postives = 367/533 (68.86%), Query Frame = 0

Query: 11  FLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSIR 70
           FL+L + LS  PS +SA    H+NF++CLS   ++    S+++HT  + SY S+LN SI+
Sbjct: 14  FLLLNLSLSHFPS-ISAQRTNHENFLKCLSHRINED--DSRIIHTSKDPSYFSILNSSIQ 73

Query: 71  NLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLSYVA-YHPFI 130
           N RF   ETPKP+ IITP   + +Q+ + C++ H + IRTRSGGHDYEGLSY+A   PF+
Sbjct: 74  NPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFV 133

Query: 131 IVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGG 190
           ++DL NLRSI++D+ N T WV+SGAT+GELYY IG+ S++LAFPAG+ PTVG+GG F GG
Sbjct: 134 VIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGG 193

Query: 191 GYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFGIVVAWKVKL 250
           GYG L+RKYGL+ADNVIDA++VDANG  LDR+ MGED FWAIRGG G SF +V++WK++L
Sbjct: 194 GYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRL 253

Query: 251 VPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKNPIA 310
           + VP++VT+   +K  E +AV +I++WQY+A+K+  D+F+  +L               A
Sbjct: 254 LDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKET--------EVYA 313

Query: 311 TFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTIESLEPLLNR 370
           +F  L+LG V +L+A +  KFPELGL   +C E+ W+ES L    G     ES+E L  R
Sbjct: 314 SFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKG-----ESMEILAKR 373

Query: 371 TPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQISKSETPF 430
             T+  S K K D+I++PIPK AI+ +W+R +A +    + +  P+G +MS+I+ +E PF
Sbjct: 374 KRTS-RSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPF 433

Query: 431 SHRAGYLYQIGYMVGWKGQNLKAK---NRHISWIRELYEYMAPFVSKSPRAAYANYRDLD 490
            HR G LY+I Y+  W  +  K K    +++ W+  +YE+M P+VSKSPR AY N+RD+D
Sbjct: 434 PHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDID 493

Query: 491 IGSNNKYG-KTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           +G       KT Y++A +WG+KYF NNF RLV VKT VDP DFF  EQSIP +
Sbjct: 494 LGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of CSPI01G25150 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 520.0 bits (1338), Expect = 2.3e-147
Identity = 259/541 (47.87%), Postives = 363/541 (67.10%), Query Frame = 0

Query: 5   SQLIPLFLVLTIVLSA-SPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPIN--SSY 64
           S+ +PLF +L +  S  + +  S+       F+ C+  ++   +P+ K    P    S +
Sbjct: 4   SKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMF 63

Query: 65  SSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLS 124
           S VL  + +NLRF K   PKP  I +P H SH+QA++ICSK   + +R RSGGHDYEGLS
Sbjct: 64  SQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 123

Query: 125 YVAY--HPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPT 184
           YV+    PFI++DL  +R ++I+I +N+AWV+SGAT+GELYYRI EKS+   FPAG+C +
Sbjct: 124 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 183

Query: 185 VGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSF 244
           +G+GGH +GG YG ++RKYGL ADNV+DA +VDANG +LDR +MGED FWAIRGG+GGSF
Sbjct: 184 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 243

Query: 245 GIVVAWKVKLVPVPAIVTICSTIKNLEDD-AVKVIHQWQYVANKLHEDIFLGIVLTGGNT 304
           GI++AWK+KLVPVP  VT+ +  K L+ D   K+I +WQ VA+KL E++F+ ++    N 
Sbjct: 244 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NV 303

Query: 305 STQGGIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQ 364
           +  GG K    ++ +LFLG    L+  +   FPELGL  +DC+E+ W+ES   I+   F 
Sbjct: 304 AGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISG--FP 363

Query: 365 TIESLEPLL-NRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGA 424
           T      LL  ++P    S K KSD++K PIP++ ++GI+++L   DI  P  ++ PYG 
Sbjct: 364 THTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGG 423

Query: 425 RMSQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRA 484
            M++I +S+ PF HR G L+++ Y+  W   + K  +RHI+WIR+LY YM P+VS +PR 
Sbjct: 424 MMAKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSNPRE 483

Query: 485 AYANYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPT 539
           AY NYRDLD+G N K  KT  KQA +WG  YF NNF RL+ +K KVDP +FFRHEQSIP 
Sbjct: 484 AYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 536

BLAST of CSPI01G25150 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 3.3e-146
Identity = 260/537 (48.42%), Postives = 364/537 (67.78%), Query Frame = 0

Query: 7   LIPLFLVLTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVHTPINSSYSSVLN 66
           ++ LF V+ I  S+S S  S      + F QCL+ +S   +PIS  +    N SYSSVL 
Sbjct: 6   ILVLFFVVFIWQSSSSSANS------ETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQ 65

Query: 67  FSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLSYVAY- 126
            +IRNLRF+   TPKP LII  +H SH+QAA+ C K H+LQ++ RSGGHDY+GLSYV Y 
Sbjct: 66  ANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYS 125

Query: 127 -HPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVGG 186
             PF ++D+ NLRS+ +D+ + TAWV++GA LGE+YY I EKS+TLA+PAGICPTVGVGG
Sbjct: 126 GKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGG 185

Query: 187 HFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVLDRESMGEDLFWAIRGGSGGSFGIVVA 246
           H SGGGYG ++RKYGL  DN IDA +VD NG +LDR+ MGEDL+WAI GG GGS+G+V+A
Sbjct: 186 HISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLA 245

Query: 247 WKVKLVPVPAIVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVL--TGGNTSTQG 306
           +K+ LV VP  VT+    + LE +A  +IH+WQ VA KL +++F+  V+    G  S+Q 
Sbjct: 246 YKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQK 305

Query: 307 GIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQTIES 366
            ++    TF ++FLG    L++ L+ +FPELGL++ DC E  W++S L   +     + S
Sbjct: 306 TVR---TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN---IQVGS 365

Query: 367 LEPLLNRTPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGARMSQI 426
            E LL +    ++  K KSDY+++PI +  +E IW+  K  ++E P   F PYG  M +I
Sbjct: 366 SETLLLQRNQPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRI 425

Query: 427 SKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAAYANY 486
           S + TPF +RAG L++I Y   W+ + L   +R++   R+LY++M PFVSK+PR ++ NY
Sbjct: 426 SSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNY 485

Query: 487 RDLDIGSNNKYGK-TSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 539
           RD+D+G N+  GK +SY +   +G KYF  NF+RLV +KT+VD  +FFR+EQSIP L
Sbjct: 486 RDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526