CSPI01G24720 (gene) Cucumber (PI 183967) v1

BLAST of CSPI01G24720 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 2.0e-156
Identity = 274/530 (51.70%), Postives = 369/530 (69.62%), Query Frame = 0

Query: 14  IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
           I+  F+S +  +S     E FL+C  +  + TN + + L+YT  +  + S+LN +IQNLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKC-FSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLR 73

Query: 74  FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
           F+   TPKPL IITP +VSHIQ TILCSK   LQIR RSGGHD EG+SY+S VPF+IVDL
Sbjct: 74  FTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDL 133

Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
            N+ S+ IDV ++ AWV++GATLGE YY I E ++ L+FPAG CPTVG GGH SGGG+G 
Sbjct: 134 RNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGA 193

Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
           LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVP 253

Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPT--AL 313
           +  TI   ++++E  + +KL++KWQ I    +K LLL       N T   GK   T  + 
Sbjct: 254 SMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSY 313

Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
           FSS F G V+ L+ ++N +FPEL + K +C ++SWI T++  +G  N        E+LL+
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLD 373

Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
           R+     +  IK DY+KKP+ E A  T+L++L  +D+ V   +F PYGG M EISES IP
Sbjct: 374 RSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIP 433

Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIG 493
           FPHRAG +Y++ Y   W+ Q  +  ++H+NWIR++Y++ TP+VS++PR  Y NYRDLD+G
Sbjct: 434 FPHRAGIMYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLG 493

Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
             N      Y+ AR+WG KYFGKNF+RLV +KTK+DP++FFRNEQSIP L
Sbjct: 494 KTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G24720 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.6e-156
Identity = 274/530 (51.70%), Postives = 368/530 (69.43%), Query Frame = 0

Query: 14  IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
           I+  F+S +  +S     E FL+C L+  + TN + + L+YT  +  + S+LN ++QNLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKC-LSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLR 73

Query: 74  FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
           F+   TPKPL I TP +VSHIQ TILCSK   LQIR RSGGHD EG+SY+S VPF+IVDL
Sbjct: 74  FTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDL 133

Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
            N+ S+ IDV ++ AWV+SGATLGE YY I E ++ L+FPAG CPTVG GGH SGGG+G 
Sbjct: 134 RNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGA 193

Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
           LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVP 253

Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPT--AL 313
           +  TI   ++++E  + +KL++KWQ I    +K LLL       N T   GK   T  + 
Sbjct: 254 SMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSY 313

Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
           FSS F G V+ L+ ++N +FPEL + K +C ++SWI T++  +G  N        E+LL+
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLD 373

Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
           R+     +  IK DY+KKP+ E A  T+L++L  +D+ V   +F PYGG M EISES IP
Sbjct: 374 RSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIP 433

Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIG 493
           FPHRAG  Y++ Y   W+ Q  +  ++H+NWIR++Y++ TP+VS++PR  Y NYRDLD+G
Sbjct: 434 FPHRAGITYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLG 493

Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
             N      Y+ AR+WG KYFGKNF+RLV +KTK+DP++FFRNEQSIP L
Sbjct: 494 KTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of CSPI01G24720 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.7e-155
Identity = 286/541 (52.87%), Postives = 371/541 (68.58%), Query Frame = 0

Query: 3   SFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYS 62
           SF+     +FF     I  S A      E FL+C  +  +  N +   L+YT  N  + S
Sbjct: 7   SFWFVCKIIFFFFSFNIQTSIA---NPRENFLKC-FSQYIPNNATNLKLVYTQNNPLYMS 66

Query: 63  VLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYV 122
           VLN +I NLRF+   TPKPL I+TPSHVSHIQ TILCSK   LQIR RSGGHD EG+SY+
Sbjct: 67  VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 126

Query: 123 SDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIG 182
           S VPF+IVDL N+RSI IDV ++ AWV++GATLGE YY + EK++ L+  AG CPTV  G
Sbjct: 127 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 186

Query: 183 GHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIV 242
           GH  GGG+G LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWA+RGGG  SFGIIV
Sbjct: 187 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 246

Query: 243 AWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQES 302
           AWK++LV VP + T+   ++ +E  + +KL++KWQ I  K DK+LLL       N T   
Sbjct: 247 AWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQ 306

Query: 303 GKTNPTAL---FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQE 362
           GK N TA+   FSS FLG V+ L+ ++N +FPEL + K +C ++SWI T++  +G  N +
Sbjct: 307 GK-NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYD 366

Query: 363 P----FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGG 422
                 E+LL+R+     + KIK DY+KKP+ E+ F  +L++L  +DI        PYGG
Sbjct: 367 TDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGG 426

Query: 423 RMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRA 482
            M EISES IPFPHRAG +Y+L Y   W+ Q  +  ++HLNWIR+IY++MTP+VSK+PR 
Sbjct: 427 IMDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRL 486

Query: 483 TYSNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPA 537
            Y NYRDLDIG+N+      Y+ AR+WG KYFGKNFDRLV +KT +DPN+FFRNEQSIP 
Sbjct: 487 AYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPP 539

BLAST of CSPI01G24720 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 3.2e-154
Identity = 277/540 (51.30%), Postives = 363/540 (67.22%), Query Frame = 0

Query: 3   SFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYS 62
           SF+     +FF +   I  S A      E FL+C  +  +  N +   L+YT  +  + S
Sbjct: 7   SFWFVCKIIFFFLSFHIQISIA---NPRENFLKC-FSKHIPNNVANPKLVYTQHDQLYMS 66

Query: 63  VLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYV 122
           +LN +IQNLRF    TPKPL I+TPS+ SHIQATILCSK   LQIR RSGGHD EG+SY+
Sbjct: 67  ILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYI 126

Query: 123 SDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIG 182
           S VPF++VDL N+ SI IDV ++ AWV++GATLGE YY I EK++ L+FP G CPTVG+G
Sbjct: 127 SQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVG 186

Query: 183 GHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIV 242
           GH SGGG+G LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWAIRGGGG +FGII 
Sbjct: 187 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 246

Query: 243 AWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQES 302
           AWK+KLV VP+  TI   ++++E    +KL +KWQ I  K DK+L+L       N T   
Sbjct: 247 AWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNH 306

Query: 303 GKTNPT--ALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 362
           GK   T    FSS F G V+ L+ ++N +FPEL + K +C E SWI T +  +G  N   
Sbjct: 307 GKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNT 366

Query: 363 F----EVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGR 422
                E+LL+R+     +  IK DY+KKP+ E A   +L++L  +D+     +  PYGG 
Sbjct: 367 ANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGI 426

Query: 423 MSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAT 482
           M EISES IPFPHRAG +Y+L Y   W+ Q  +  ++H+NW+R +Y++ TP+VS++PR  
Sbjct: 427 MEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 486

Query: 483 YSNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
           Y NYRDLD+G  N      Y+ AR+WG KYFGKNF+RLV +KTK+DPN+FFRNEQSIP L
Sbjct: 487 YLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CSPI01G24720 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 7.9e-153
Identity = 271/530 (51.13%), Postives = 360/530 (67.92%), Query Frame = 0

Query: 14  IVLSFISPSWAVS-TKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQNLR 73
           I+  F+S +  +S     E FL+C  +  +  N +    IYT  +  + SVLN +IQNLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKC-FSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLR 73

Query: 74  FSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIVDL 133
           F+   TPKPL I+TPS+VSHIQA+ILCSK   LQIR RSGGHD EGLSY+S VPF IVDL
Sbjct: 74  FTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDL 133

Query: 134 INLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGFGW 193
            N+ ++ +D+ ++ AWV++GATLGE YY I E ++  +FP G CPTVG+GGH SGGG+G 
Sbjct: 134 RNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGA 193

Query: 194 LMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVRVP 253
           LMR YGLAADN+IDA  V+ +GKV DR+SMG+DLFWAIRGGGG +FGII AWK+KLV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVP 253

Query: 254 ATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTNPT--AL 313
           +  TI   ++++E    +KL +KWQ I  K DK+L+L       N T   GK   T    
Sbjct: 254 SKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGY 313

Query: 314 FSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPF----EVLLN 373
           FSS FLG V+ L+ ++N +FPEL + K +C E+SWI T +  +G  N        E+LL+
Sbjct: 314 FSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLD 373

Query: 374 RTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIP 433
           R+     +  IK DY+KK + E A   +L++L  +++ V   +  PYGG M EISES IP
Sbjct: 374 RSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIP 433

Query: 434 FPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIG 493
           FPHRAG +Y+L Y   W+ Q  +  ++H+NW+R +Y++ TP+VS++PR  Y NYRDLD+G
Sbjct: 434 FPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLG 493

Query: 494 MNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
             N      Y+ AR+WG KYFGKNF+RLV +KTK DPN+FFRNEQSIP L
Sbjct: 494 KTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CSPI01G24720 vs. ExPASy TrEMBL
Match: A0A1S3BE36 (cannabidiolic acid synthase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488617 PE=3 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 9.9e-300
Identity = 509/544 (93.57%), Postives = 525/544 (96.51%), Query Frame = 0

Query: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
           MKSF LTPFALFFIVL+F+SPSWAVSTK HEAFLQCL N SLTTNYSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
           YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQA I+CSKSHALQIRIRSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
           YVSDVPFI+VDLINLRSITIDVE ENAWVQSGATLGEFYYRIGEKS+TLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
           IGGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMG+DLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
           IVAWKVKLVRVPATVTICGS+RSLEEEDTIKLIH+WQYITN LDKNLLLGISLTGGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
           ESGKTNP ALFSSFFLGKVNELMPIL+T FPELNLSKEECSEMSWIKTVL MAGFP  EP
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLLNRTP FGLSTKIKSDYIKKPMSEAAFKTMLKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNY 480
           S+SE PFPHR GNIYKLGYYVKWKDQSIDAEKRHL+WIRDIYDYMTPFVSKSPRATYSNY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
           RDLDIG+NNKYGKATYSHARVWGFKYFGKNFDRLVH+KT IDPNDFFRNEQSIPAL +IK
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDIK 542

Query: 541 YSAI 545
           YS I
Sbjct: 543 YSVI 546

BLAST of CSPI01G24720 vs. ExPASy TrEMBL
Match: A0A5D3DMS2 (Cannabidiolic acid synthase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5482G00040 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.1e-285
Identity = 490/542 (90.41%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
           MKSF LTPFALFFIVL+F+SPSWAVSTK HEAFLQCL N SLTTNYSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
           YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQA I+CSKSHALQIRIRSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
           YVSDVPFI+VDLINLRSITIDVE ENAWVQSGATLGEFYYRIGEKS+TLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
           IGGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMG+DLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
           IVAWKVKLVRVPATVTICGS+RSLEEEDTIKLIH+WQYITN LDKNLLLGISLTGGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
           ESGKTNP ALFSSFFLGKVNELMPIL+T FPELNLSKEECSEMSWIKTVL MAGFP  EP
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLLNRTP FGLSTKIKSDYIKKPMSEAAFKTMLKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNY 480
           S+SE PFPHR GNIYKLGYYVKWKDQSIDAEKRHL+WIRDIYDYMTPFVSKSPRATYSNY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
           RDLDIG+NNKYGKATYSHARVWGFKYFGKNFDR    K   D  D  R    +P ++  K
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRKGVDKASSDTRDPSREPIPMPIMQLSK 542

Query: 541 YS 543
            S
Sbjct: 543 ES 544

BLAST of CSPI01G24720 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 7.8e-212
Identity = 360/541 (66.54%), Postives = 434/541 (80.22%), Query Frame = 0

Query: 1   MKSFFLTPFALFFIVLSFISPS--WAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINS 60
           MKSF L P A  F     +S S  WA S   HE FLQCL  +S  +N SIS  IYTP N 
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHS-PSNNSISKFIYTPANF 60

Query: 61  SFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEG 120
           S+ S+LNFSIQNLRFS  +TPKPL +I PSH+SHIQA ++CSK+H LQIR RSGGHDFEG
Sbjct: 61  SYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEG 120

Query: 121 LSYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPT 180
           LSYVS +PFI++DLI+LRSITID +N  AWVQSGATLGE YYRI +KSQTLAFPAGSCPT
Sbjct: 121 LSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPT 180

Query: 181 VGIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSF 240
           VG+GGHLSGGG+G+L+RKYGLAAD+VIDA  VDANG++ +RESM DDLFW IRGGGGGSF
Sbjct: 181 VGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSF 240

Query: 241 GIIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGN- 300
           GI+VAWK++LV VP T+T C   R+L + D +KLIH+WQY+ +KL+ NLLLGISLTGG+ 
Sbjct: 241 GIVVAWKLRLVPVPTTLTTCTVNRTL-DRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHI 300

Query: 301 STQESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPN 360
            TQ+ GKTNP A F ++FLGKV+EL+ I+NT FPEL L+KE+C E SWI++ L  AGF  
Sbjct: 301 LTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSA 360

Query: 361 QEPFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRM 420
            E  E LL R P   +STKIKSDY+K+P+SEA FK + +RL  QDIE+AQ+MF+PYGGRM
Sbjct: 361 GESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRM 420

Query: 421 SEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY 480
           SE SESE P+PHRA N+YK+GY VKW++QS  AEKRHLNW+R+IYDYM P+V+KSPRA Y
Sbjct: 421 SEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAY 480

Query: 481 SNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALK 539
            NYRDLDIG NN+Y K  Y  A+VWG KYFGKNF+RLV +KT++DP+DFFR+EQSIP   
Sbjct: 481 VNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 539

BLAST of CSPI01G24720 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.5e-210
Identity = 359/529 (67.86%), Postives = 428/529 (80.91%), Query Frame = 0

Query: 8   PFALFFIVLSFISPSWA-VSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNF 67
           P  L FI++   SP WA  S   H+ FL CL ++S  T Y IS L+YTPINSS+  VLNF
Sbjct: 5   PLTLIFIIVLSSSPLWASPSLHKHQHFLHCLSHHSPHT-YPISKLVYTPINSSYSPVLNF 64

Query: 68  SIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVP 127
           SI+NL FS  QTPKPL IITPSH+SHIQA I+CSK+H LQIR RSGGHDFEGLSYV++ P
Sbjct: 65  SIRNLIFSNPQTPKPLLIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQP 124

Query: 128 FIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLS 187
           FIIVDLINLRSI +DV+   AWVQSGATLGE YYRIGE S++LAFPAG CPTVG+GGH S
Sbjct: 125 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHFS 184

Query: 188 GGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKV 247
           GGG+G ++RKYGLAADNV+DA  VDANG+V+DRESMG+DLFWAIRGGGGGSFGI+VAWKV
Sbjct: 185 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 244

Query: 248 KLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKTN 307
           KLV VPA VT+C + R + E+D IKLIH+WQY+ N+LD NL LGI LTGGN +   G TN
Sbjct: 245 KLVMVPAVVTMCSTNRDV-EDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITN 304

Query: 308 PTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIK-TVLTMAGFPNQEPFEVLL 367
           PTA F S +LGK++EL+ IL  NF EL L+K++C E SWI+ T++   G    EP E LL
Sbjct: 305 PTATFFSLYLGKLDELITILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALL 364

Query: 368 NRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEI 427
           NRTPP   STK KSDY+K P+ EA  + + +RLKAQDIEV QI+FIPYGGRMS+ISESE 
Sbjct: 365 NRTPPTIESTKFKSDYVKDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESET 424

Query: 428 PFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDI 487
           PF HRAGN+YK+GY V WK+QS+DA+ +H+NWIR++Y+YMTPFVSKSPRA Y+NYRDLDI
Sbjct: 425 PFSHRAGNLYKIGYAVSWKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDI 484

Query: 488 GMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIP 535
           G NNKYGK +Y  A VWG KYFG NF+RLV++KTK+DP+DFFR+EQSIP
Sbjct: 485 GTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of CSPI01G24720 vs. ExPASy TrEMBL
Match: A0A5D3C112 (Cannabidiolic acid synthase-like 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00400 PE=3 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 3.6e-209
Identity = 357/544 (65.62%), Postives = 435/544 (79.96%), Query Frame = 0

Query: 1   MKSF--FLTPFAL--FFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPI 60
           MK F     PF L  F IV+   + + +  T+  ++F+QCL +     +  I+N+IYTP 
Sbjct: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60

Query: 61  NSSFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDF 120
           NSS+ S+LNFSIQNLRF  K   KPLAIITPSHVSHIQA ++CSKSH  QIR RSGGHDF
Sbjct: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120

Query: 121 EGLSYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSC 180
           EGLSY+S VPF++VDLINL SI+IDVEN+NAWV+SGATLGE YYRI EKSQTLAFPAGSC
Sbjct: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180

Query: 181 PTVGIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGG 240
           PTVG+GGHLSGGG+GWL+RKYGLAAD+VIDA  VDANGKV+++ESMG+DLFWAIRGGGGG
Sbjct: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240

Query: 241 SFGIIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGG 300
            FGI+VAWKVKLV VPA VTIC + R+LEE+  +KLIH+WQY+  KLD NLLLGISLTGG
Sbjct: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300

Query: 301 NSTQ-ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGF 360
             T+ E GK NP A FS  FLGK  E++ IL   FP+L+L+KE+C EMSWI++VL M  F
Sbjct: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360

Query: 361 PNQEPFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGG 420
              +P EVLLNRT  +   +KIKSDY+K+ +   A K M +RLK++D+E++QIMF+PYGG
Sbjct: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420

Query: 421 RMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRA 480
           +MSE+ +SE PF HR+   Y +GY  KWK+ S++AE+RHLNWIR+IYDYMTPFVSKSPRA
Sbjct: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480

Query: 481 TYSNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPA 540
            Y NYRDLDIG NNKYG+ +Y  ARVWG KYFGKNFD+LV++KTK+DP+DFFR++QSIP 
Sbjct: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540

BLAST of CSPI01G24720 vs. NCBI nr
Match: XP_011648520.1 (cannabidiolic acid synthase [Cucumis sativus] >KAE8653301.1 hypothetical protein Csa_023300 [Cucumis sativus])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 542/544 (99.63%), Postives = 542/544 (99.63%), Query Frame = 0

Query: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
           MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF
Sbjct: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60

Query: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
           YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS
Sbjct: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120

Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
           YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG
Sbjct: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180

Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
           IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240

Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
           IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ
Sbjct: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300

Query: 301 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
           ESGK NPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP
Sbjct: 301 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360

Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI
Sbjct: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420

Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNY 480
           SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY NY
Sbjct: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNY 480

Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
           RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK
Sbjct: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540

Query: 541 YSAI 545
           YSAI
Sbjct: 541 YSAI 544

BLAST of CSPI01G24720 vs. NCBI nr
Match: XP_008445658.1 (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis melo])

HSP 1 Score: 1038.5 bits (2684), Expect = 2.1e-299
Identity = 509/544 (93.57%), Postives = 525/544 (96.51%), Query Frame = 0

Query: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
           MKSF LTPFALFFIVL+F+SPSWAVSTK HEAFLQCL N SLTTNYSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
           YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQA I+CSKSHALQIRIRSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
           YVSDVPFI+VDLINLRSITIDVE ENAWVQSGATLGEFYYRIGEKS+TLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
           IGGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMG+DLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
           IVAWKVKLVRVPATVTICGS+RSLEEEDTIKLIH+WQYITN LDKNLLLGISLTGGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
           ESGKTNP ALFSSFFLGKVNELMPIL+T FPELNLSKEECSEMSWIKTVL MAGFP  EP
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLLNRTP FGLSTKIKSDYIKKPMSEAAFKTMLKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNY 480
           S+SE PFPHR GNIYKLGYYVKWKDQSIDAEKRHL+WIRDIYDYMTPFVSKSPRATYSNY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
           RDLDIG+NNKYGKATYSHARVWGFKYFGKNFDRLVH+KT IDPNDFFRNEQSIPAL +IK
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDIK 542

Query: 541 YSAI 545
           YS I
Sbjct: 543 YSVI 546

BLAST of CSPI01G24720 vs. NCBI nr
Match: KAA0047118.1 (cannabidiolic acid synthase-like 1 [Cucumis melo var. makuwa] >TYK24888.1 cannabidiolic acid synthase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 990.3 bits (2559), Expect = 6.4e-285
Identity = 490/542 (90.41%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60
           MKSF LTPFALFFIVL+F+SPSWAVSTK HEAFLQCL N SLTTNYSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120
           YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQA I+CSKSHALQIRIRSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180
           YVSDVPFI+VDLINLRSITIDVE ENAWVQSGATLGEFYYRIGEKS+TLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240
           IGGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMG+DLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300
           IVAWKVKLVRVPATVTICGS+RSLEEEDTIKLIH+WQYITN LDKNLLLGISLTGGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360
           ESGKTNP ALFSSFFLGKVNELMPIL+T FPELNLSKEECSEMSWIKTVL MAGFP  EP
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLLNRTP FGLSTKIKSDYIKKPMSEAAFKTMLKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNY 480
           S+SE PFPHR GNIYKLGYYVKWKDQSIDAEKRHL+WIRDIYDYMTPFVSKSPRATYSNY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540
           RDLDIG+NNKYGKATYSHARVWGFKYFGKNFDR    K   D  D  R    +P ++  K
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRKGVDKASSDTRDPSREPIPMPIMQLSK 542

Query: 541 YS 543
            S
Sbjct: 543 ES 544

BLAST of CSPI01G24720 vs. NCBI nr
Match: XP_038895208.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 944.9 bits (2441), Expect = 3.1e-271
Identity = 463/545 (84.95%), Postives = 500/545 (91.74%), Query Frame = 0

Query: 1   MKSFFLTPFALFFIVLSFISPSWAV-STKTHEAFLQCLLNNSLTTNYSISNLIYTPINSS 60
           MKSF LTPFAL FIVLS ISPSW V STK HE F+QCLLNNSL+T Y ISNLIYTPINSS
Sbjct: 1   MKSFLLTPFALTFIVLS-ISPSWVVFSTKKHENFVQCLLNNSLST-YPISNLIYTPINSS 60

Query: 61  FYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGL 120
           + S+LNFSIQNLRFS K+TPKPLAIITPSHVSHIQA I+CSKSHALQIR RSGGHDFEGL
Sbjct: 61  YSSILNFSIQNLRFSSKETPKPLAIITPSHVSHIQAAIICSKSHALQIRTRSGGHDFEGL 120

Query: 121 SYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTV 180
           SY+SDVPF++VDLINL+SI IDVENE AWVQSGATLGE YYRI EKS+TLAFPAGSCPTV
Sbjct: 121 SYISDVPFVVVDLINLKSIAIDVENETAWVQSGATLGELYYRIAEKSRTLAFPAGSCPTV 180

Query: 181 GIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFG 240
           G+GGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMG+DLFWAIRGGGGGSFG
Sbjct: 181 GVGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNST 300
           I+VAWKVKLVRVPATVTICG  R+LEEEDTIKLIH+WQY+TNKLD+NLLLGISL GGNST
Sbjct: 241 IVVAWKVKLVRVPATVTICGINRNLEEEDTIKLIHRWQYVTNKLDENLLLGISLIGGNST 300

Query: 301 QESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQE 360
           QE GKTNP ALFSSFFLGKVNEL+ ILNTNF EL L+KE+CSEMSWIK+VL MAGFP QE
Sbjct: 301 QEGGKTNPMALFSSFFLGKVNELVTILNTNFSELGLAKEDCSEMSWIKSVLIMAGFPKQE 360

Query: 361 PFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSE 420
             EVLLNR PP GLSTKIKSDY+K+P+S+A+FKTM KRLKA+DIEVAQIMFIPYGGRMSE
Sbjct: 361 ALEVLLNRAPPSGLSTKIKSDYVKEPISKASFKTMFKRLKAEDIEVAQIMFIPYGGRMSE 420

Query: 421 ISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSN 480
           ISES  PFPHRAGNIYKLGYYVKWK+QSIDAEKRHLNW+R IYDYMTPFVSKSPRA Y+N
Sbjct: 421 ISESLTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWVRVIYDYMTPFVSKSPRAAYNN 480

Query: 481 YRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 540
           YRDLDIG NNK GK +Y+ ARVWG KYFGKNFDRLVH+KTKIDP +FFRNEQSIP L +I
Sbjct: 481 YRDLDIGTNNKCGKTSYNRARVWGLKYFGKNFDRLVHVKTKIDPTNFFRNEQSIPTLTDI 540

Query: 541 KYSAI 545
           KYS I
Sbjct: 541 KYSTI 543

BLAST of CSPI01G24720 vs. NCBI nr
Match: XP_004135742.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653322.1 hypothetical protein Csa_023372 [Cucumis sativus])

HSP 1 Score: 753.8 bits (1945), Expect = 1.0e-213
Identity = 359/530 (67.74%), Postives = 433/530 (81.70%), Query Frame = 0

Query: 11  LFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNFSIQN 70
           +  +VLS  +PS+  ST+  E+F+QCL +   ++   I+NLIYTP NSS+ S+LNFSIQN
Sbjct: 19  IIVVVLSTATPSFC-STQESESFVQCLFDYGSSSTNPIANLIYTPTNSSYSSILNFSIQN 78

Query: 71  LRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVSDVPFIIV 130
           LRF  K  PKP AIITP HVSHIQA ++CSKSH  QIR RSGGHDFEGLSY+SD+PF++V
Sbjct: 79  LRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDIPFVLV 138

Query: 131 DLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHLSGGGF 190
           DLINL+SI+IDVEN+NAWV SGATLGE YYRI EKSQTLAFPAGSCPTVG+GGHLSGGG+
Sbjct: 139 DLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPAGSCPTVGVGGHLSGGGY 198

Query: 191 GWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWKVKLVR 250
           GWL+RKYGLAAD+VIDA  VDANGKV+++ESMG+DLFWAIRGGGGGSFGI+VAWKVKLV 
Sbjct: 199 GWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVKLVE 258

Query: 251 VPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ-ESGKTNPTA 310
           VPA VTIC + R+LEE+  +KLIH+WQY+  KLD NLLLGISLTGG  T+ E GK NP A
Sbjct: 259 VPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIA 318

Query: 311 LFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLLNRTP 370
            FS  FLGK  E++ IL   FP+L L+KE+C EMSWI++VL M  F  ++P EVLLNR+ 
Sbjct: 319 SFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKEDPLEVLLNRSR 378

Query: 371 PFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEIPFPH 430
            +   +KIKSDY+K+ +   A K M +RLK+QD+E++QIMF+PYGG+MSE+ +SE PF H
Sbjct: 379 LYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVPYGGKMSEVDDSETPFSH 438

Query: 431 RAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRDLDIGMNN 490
           R+   Y +GY  KWK+ S++AEK HLNWIR+IYDYMTPFVSKSPRA Y NYRDLDIG NN
Sbjct: 439 RSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNN 498

Query: 491 KYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 540
           KYG+ +Y  ARVWG KYFGKNFDRLVH+KTK+DP+DFFR+EQSIP L  I
Sbjct: 499 KYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQSIPTLSGI 547

BLAST of CSPI01G24720 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 513.5 bits (1321), Expect = 2.1e-145
Identity = 252/537 (46.93%), Postives = 365/537 (67.97%), Query Frame = 0

Query: 10  ALFFIVLSF---ISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFYSVLNF 69
           +  F +L F   ISP  +     HE FL+C L+  +  N     +I+T  +SSF+S+L+ 
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKC-LSYRMNDNTVEPKVIHTSKDSSFFSILDS 64

Query: 70  SIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVS-DV 129
           SIQN RFS  +TPKP++IITP   S +Q  I C++ H + +R RS GH +EGLSY++ + 
Sbjct: 65  SIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNK 124

Query: 130 PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGIGGHL 189
           PF ++DL NLRSI++DV+N   WVQ+GAT GE YY IG+ +++LAFPAG  PTVG+GG  
Sbjct: 125 PFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQF 184

Query: 190 SGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGIIVAWK 249
           SGGG+G L+RKYGLAADN+IDA  VDA+G++ DR++MG+D FWAIRGGGG SFG+I++WK
Sbjct: 185 SGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWK 244

Query: 250 VKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQESGKT 309
           VKLV VP+T+T+   Q++ ++E  +++I KWQY  +K+  +L +  +L      + S K 
Sbjct: 245 VKLVDVPSTITVFKVQKTSKKE-AVRIIKKWQYAADKVPDDLFIRTTL------ERSNKN 304

Query: 310 NPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEPFEVLL 369
              ALF+  ++G VN L+ ++   FPEL L KE C EMSWI++VL  A FP  E   VL 
Sbjct: 305 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLT 364

Query: 370 NRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEISESEI 429
           NR     LS K K D++++P+ EAA + + +RL+A +  + +I+  P+GG+MSE++E E 
Sbjct: 365 NRERT-SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 424

Query: 430 PFPHRAGNIYKLGYYVKWK---DQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSNYRD 489
           PFPHR GN+Y++ Y   W+   D++     ++L W+  +Y++MTP+VSKSPR  Y N++D
Sbjct: 425 PFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKD 484

Query: 490 LDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNI 540
           +D+GM     K  Y   + WG KYF  NF+RLV +KT++DP DFF +EQSIP +  +
Sbjct: 485 MDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVNKV 532

BLAST of CSPI01G24720 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 5.8e-143
Identity = 249/535 (46.54%), Postives = 359/535 (67.10%), Query Frame = 0

Query: 2   KSFFLTPFALFFIVLSFISPSWAVST--KTHEAFLQCLLNNSLTTNYSISNLIYTPINSS 61
           +SFF +    F      +S    VS       +FLQC L+  L  +  +S +I+TP ++S
Sbjct: 4   QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQC-LSLQLNDSNIVSKVIHTPNDTS 63

Query: 62  FYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGL 121
           F SVL  SIQN RFS    PKP+ I+TP   S +Q+ + C++   + IR RSGGHD+EGL
Sbjct: 64  FSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGL 123

Query: 122 SYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTV 181
           SYV+  PF+I+DL NLRSIT+DV+N + WVQ+GAT+GE YY IG+K++TLAFPAG CPTV
Sbjct: 124 SYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTV 183

Query: 182 GIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFG 241
           G+GGH SGGG+G L+RK+GLAAD+VIDA  VDA G++ +R  MG+D FWAIRGGGG SF 
Sbjct: 184 GVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFC 243

Query: 242 IIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNST 301
           ++++WK+ L+ VP+TVT+    +   E+  +K+IH+WQ++ +K+  +L + + L      
Sbjct: 244 VVLSWKIGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVML------ 303

Query: 302 QESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQE 361
            +  K    A F   +LG V  L+ ++N  FPEL L +++C+EMSWI++V+  A    +E
Sbjct: 304 -QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEE 363

Query: 362 PFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSE 421
           P  VL  RT    L+ K KSD++++PM + A   + +RL+  + E AQ++F P+GG+MSE
Sbjct: 364 PINVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSE 423

Query: 422 ISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYSN 481
           I++ E PFPHR GNIY++ Y   W+    D +++++ W+  +YD M+ FV+KSPR  Y N
Sbjct: 424 IADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYIN 483

Query: 482 YRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIP 535
            RDLD+GM     ++ Y   + WG KYF  NF+RLV +KT +DP+DFF +EQSIP
Sbjct: 484 LRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G24720 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 503.4 bits (1295), Expect = 2.2e-142
Identity = 255/542 (47.05%), Postives = 358/542 (66.05%), Query Frame = 0

Query: 8   PFALFFIVLSFISPSWAVSTKT----HEAFLQCLLNNSLTTNYSISNLIYTPIN--SSFY 67
           P  LF I++ + S      T +     + F+ C+  N+    + +    + P    S F 
Sbjct: 6   PLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNT-HVYFPLEKTFFAPTKNVSMFS 65

Query: 68  SVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSY 127
            VL  + QNLRF +K  PKP  I +P H SH+QA+I+CSK   + +R+RSGGHD+EGLSY
Sbjct: 66  QVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSY 125

Query: 128 VS--DVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTV 187
           VS  D PFI++DL  +R + I++++ +AWVQSGAT+GE YYRI EKS+   FPAG C ++
Sbjct: 126 VSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSL 185

Query: 188 GIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFG 247
           GIGGH++GG +G +MRKYGL ADNV+DA  VDANGK+ DR +MG+D FWAIRGG GGSFG
Sbjct: 186 GIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFG 245

Query: 248 IIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNL----LLGISLTG 307
           II+AWK+KLV VP TVT+    ++L+++   K+I KWQ + +KL + L    L  ++ TG
Sbjct: 246 IILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTG 305

Query: 308 GNSTQESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGF 367
           GN T        T  +++ FLG    LM ++  +FPEL L+ ++C EMSW++++  ++GF
Sbjct: 306 GNKT-------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGF 365

Query: 368 PNQEPFEVLLNRTPPF-GLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYG 427
           P   P  VLL    PF  +S K KSD++K P+ E+  + + K+L  +DI +  +++ PYG
Sbjct: 366 PTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYG 425

Query: 428 GRMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPR 487
           G M++I ES+IPFPHR G ++K+ Y   W D S     RH+NWIRD+Y YMTP+VS +PR
Sbjct: 426 GMMAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPR 485

Query: 488 ATYSNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIP 537
             Y NYRDLD+G N K  K     A+VWG  YF  NF+RL+ +K K+DP +FFR+EQSIP
Sbjct: 486 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536

BLAST of CSPI01G24720 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 497.7 bits (1280), Expect = 1.2e-140
Identity = 251/539 (46.57%), Postives = 354/539 (65.68%), Query Frame = 0

Query: 6   LTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPIN--SSFYSV 65
           ++ FAL+F   SF + +   ST   + F++CL  N+    +++    +TP    S F  V
Sbjct: 13  ISVFALYF---SFYTITLTSSTSLQDDFIKCLYRNT-NVRFTLDKTFFTPERNASIFTEV 72

Query: 66  LNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSYVS 125
           L  + QN R+  K  PKP  I  P H SH+QA+++CSK   +  R+RSGGHD+EG+SYVS
Sbjct: 73  LESTAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVS 132

Query: 126 DV--PFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVGI 185
            +  PF+++DL  LR I +D+++ +AWV++GAT+GE YYRI EKS+   FPAG  P++GI
Sbjct: 133 QIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGI 192

Query: 186 GGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGII 245
           GGH++GG +G LMRKYGLAADNV+DA  VDANGK+ DR SMG+DLFWAIRGG GGSFGII
Sbjct: 193 GGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGII 252

Query: 246 VAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLT-GGNSTQ 305
           ++WK+KLV VP T+T+    ++ E++ + K++ KWQ I + L   L L +  T  GN   
Sbjct: 253 LSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGNKAN 312

Query: 306 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQ-- 365
           ++     T  +   FLG+   LM ++  +FPEL L++++C EMSWI +++  +GFP    
Sbjct: 313 KT----VTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPP 372

Query: 366 EPFEVLLNRTPPFG-LSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRM 425
            P E+LL    P G +  K KSD+ KKP+     + M K+L  +D   A +++ PYGG+M
Sbjct: 373 PPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEED--AALVIWTPYGGKM 432

Query: 426 SEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY 485
            +I ESEIPFPHR G  + + YY  W D S     R   WIR++Y YMTP+VS +PR  Y
Sbjct: 433 DKIPESEIPFPHRNGTNFMIQYYRSWSD-SEKRPNRRTKWIRELYGYMTPYVSSNPRQAY 492

Query: 486 SNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 537
            NYRDLD+G N    K+ +  A++WG  YF  NF+RLV +K+K+DP++FFR+EQSIP L
Sbjct: 493 VNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of CSPI01G24720 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 496.5 bits (1277), Expect = 2.7e-140
Identity = 253/543 (46.59%), Postives = 361/543 (66.48%), Query Frame = 0

Query: 5   FLTPFALFFIVLSFI---SPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSFY 64
           FL    ++F++L+      PS +     HE FL+CL   S   N   S +I+T  + S++
Sbjct: 6   FLLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCL---SHRINEDDSRIIHTSKDPSYF 65

Query: 65  SVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLSY 124
           S+LN SIQN RF   +TPKP++IITP   + +Q+TI C++ H + IR RSGGHD+EGLSY
Sbjct: 66  SILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSY 125

Query: 125 VS-DVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 184
           ++   PF+++DL NLRSIT+DV+N   WVQSGAT+GE YY IG+ S++LAFPAG  PTVG
Sbjct: 126 MAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVG 185

Query: 185 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 244
           IGG   GGG+G LMRKYGL+ADNVIDA  VDANG   DR+ MG+D FWAIRGGGG SF +
Sbjct: 186 IGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSV 245

Query: 245 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 304
           +++WK++L+ VP+ VT+    ++ E+E  + +I+KWQYI +K+  +L +   L       
Sbjct: 246 VLSWKIRLLDVPSVVTVFKVVKTSEKE-AVSIINKWQYIADKVPNDLFIRAML------- 305

Query: 305 ESGKTNPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 364
              +T   A F   +LG V++L+ ++   FPEL L    C EMSWI++VL    F   E 
Sbjct: 306 -QKETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGES 365

Query: 365 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 424
            E+L  R      S K K D+I++P+ + A + + +R +A +  +A+I+  P+GG+MSEI
Sbjct: 366 MEILAKRKRT-SRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 425

Query: 425 SESEIPFPHRAGNIYKLGYYVKW---KDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATY 484
           +++EIPFPHR GN+Y++ Y   W   +D++    +++L W+  +Y++MTP+VSKSPR  Y
Sbjct: 426 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 485

Query: 485 SNYRDLDIGMNNKYG-KATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPAL 540
            N+RD+D+GM      K  Y  A+VWG KYF  NFDRLV +KT +DP DFF +EQSIP +
Sbjct: 486 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 532