CSPI01G24640 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G24640
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	AB hydrolase-1 domain-containing protein
Location	Chr1: 20034650 .. 20034992 (+)
RNA-Seq Expression	CSPI01G24640
Synteny	CSPI01G24640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCGTGGTCGGCGATCCCACAAAAAAGAAGACCCACTTTGTGCTTGTTCATGGATCTTTCCATGGCGCTTGGTCATGGTACAAGCTCTCCACCATTCTCCAATCGTGGGGCCACAACGTCACCGCCCTCGACATGGCTGCCTCCAGGCGTCGACCCCATACAGCCTAAGACTATATCCTCAGCCTTTCAGTACTTTCACCCCTTGATGGAGTTCTTGGCTGGTTTGTCCTCCGACGAGAGGGTGGTCCTCATTGGCCACAGCTTCGGCGGCAATGGAGGATTTCCCAGAGAAGATCTCTGTTGGTGTCTTTGTCACCGCCACCATGCCTGGTCCCAATCTTAG

mRNA sequence

CCCGTGGTCGGCGATCCCACAAAAAAGAAGACCCACTTTGTGCTTGTTCATGGATCTTTCCATGGCGCTTGGTCATGGTACAAGCTCTCCACCATTCTCCAATCGTGGGGCCACAACGTCACCGCCCTCGACATGGCTGCCTCCAGGCGTCGACCCCATACAGCCTAAGACTATATCCTCAGCCTTTCAGTACTTTCACCCCTTGATGGAGTTCTTGGCTGGTTTGTCCTCCGACGAGAGGGTGGTCCTCATTGGCCACAGCTTCGGCGGCAATGGAGGATTTCCCAGAGAAGATCTCTGTTGGTGTCTTTGTCACCGCCACCATGCCTGGTCCCAATCTTAG

Coding sequence (CDS)

ATGGATCTTTCCATGGCGCTTGGTCATGGTACAAGCTCTCCACCATTCTCCAATCGTGGGGCCACAACGTCACCGCCCTCGACATGGCTGCCTCCAGGCGTCGACCCCATACAGCCTAAGACTATATCCTCAGCCTTTCAGTACTTTCACCCCTTGATGGAGTTCTTGGCTGGTTTGTCCTCCGACGAGAGGGTGGTCCTCATTGGCCACAGCTTCGGCGGCAATGGAGGATTTCCCAGAGAAGATCTCTGTTGGTGTCTTTGTCACCGCCACCATGCCTGGTCCCAATCTTAG

Protein sequence

MDLSMALGHGTSSPPFSNRGATTSPPSTWLPPGVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNGGFPREDLCWCLCHRHHAWSQS*

Homology

[Close]

BLAST of CSPI01G24640 vs. ExPASy Swiss-Prot
Match: Q9LVL9 (Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.1e-04
Identity = 19/42 (45.24%), Postives = 28/42 (66.67%), Query Frame = 0


Query: 33 GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGG 75
          G++P  P T+SS   Y  PL  FL+ L +D++V+L+ HS GG
Sbjct: 42 GINPTDPNTVSSLDDYDEPLYAFLSQLPNDQKVILVSHSVGG 83

[Close]

BLAST of CSPI01G24640 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-04
Identity = 22/43 (51.16%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 33 GVDPIQPKT-ISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGG 75
          G+D  +  T IS+  QY  PLM+ +  L +DE+VVL+GHSFGG
Sbjct: 46 GIDTTRSITDISTCEQYSEPLMQLMTSLPNDEKVVLVGHSFGG 88

[Close]

BLAST of CSPI01G24640 vs. ExPASy Swiss-Prot
Match: O80475 (Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 4.2e-04
Identity = 19/42 (45.24%), Postives = 28/42 (66.67%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGG 75
           G+D      IS+  QY  PL++ +  L +DE+VVL+GHS+GG
Sbjct: 64  GIDTRSITDISTCEQYSEPLIQLMTSLPNDEKVVLVGHSYGG 105

[Close]

BLAST of CSPI01G24640 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 4.2e-04
Identity = 20/42 (47.62%), Postives = 28/42 (66.67%), Query Frame = 0


Query: 33 GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGG 75
          G++P +   I +   Y  PLME +A +  DE+VVL+GHSFGG
Sbjct: 49 GINPRRLDEIHTFRDYSEPLMEVMASIPPDEKVVLLGHSFGG 90

[Close]

BLAST of CSPI01G24640 vs. ExPASy Swiss-Prot
Match: Q8S9K8 (Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 7.1e-04
Identity = 20/44 (45.45%), Postives = 26/44 (59.09%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           GVD  Q   +     Y  PLM F+  L  +E+VVL+GHS+GG G
Sbjct: 58  GVDTRQLHEVRLVSAYLEPLMSFMESLPENEKVVLVGHSYGGIG 101

[Close]

BLAST of CSPI01G24640 vs. ExPASy TrEMBL
Match: A0A5D3BD36 (Salicylic acid-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10G00510 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.3e-13
Identity = 38/44 (86.36%), Postives = 41/44 (93.18%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           G+DPIQPKT+SSAFQYFHPL EFLAGL SDERVVL+GHSFGG G
Sbjct: 151 GIDPIQPKTVSSAFQYFHPLTEFLAGLPSDERVVLVGHSFGGIG 194

[Close]

BLAST of CSPI01G24640 vs. ExPASy TrEMBL
Match: A0A1S3BAM5 (salicylic acid-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103488011 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.3e-13
Identity = 38/44 (86.36%), Postives = 41/44 (93.18%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           G+DPIQPKT+SSAFQYFHPL EFLAGL SDERVVL+GHSFGG G
Sbjct: 73  GIDPIQPKTVSSAFQYFHPLTEFLAGLPSDERVVLVGHSFGGIG 116

[Close]

BLAST of CSPI01G24640 vs. ExPASy TrEMBL
Match: A0A6J1KLA2 (LOW QUALITY PROTEIN: putative inactive methylesterase 20 OS=Cucurbita maxima OX=3661 GN=LOC111495596 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 8.2e-11
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           GVDP QPKTISSAFQYF PL EFLAG  S ERVVL+GHSFGG G
Sbjct: 68  GVDPRQPKTISSAFQYFQPLTEFLAGFPSGERVVLVGHSFGGLG 111

[Close]

BLAST of CSPI01G24640 vs. ExPASy TrEMBL
Match: A0A6J1CDF0 (salicylic acid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC111010668 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 8.5e-08
Identity = 31/44 (70.45%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           G+DP Q KTISS F YF PL E LA L S ERVVL+GHSFGG G
Sbjct: 76  GIDPRQAKTISSTFHYFQPLTELLAALPSGERVVLVGHSFGGLG 119

[Close]

BLAST of CSPI01G24640 vs. ExPASy TrEMBL
Match: A0A6J1CFI6 (methylesterase 3-like OS=Momordica charantia OX=3673 GN=LOC111010761 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.2e-07
Identity = 30/44 (68.18%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           G+DP QPKTISSA  YF PL +FLA L   ERVVL+ HSFGG G
Sbjct: 72  GIDPRQPKTISSAADYFQPLNDFLAALPPGERVVLVAHSFGGLG 115

[Close]

BLAST of CSPI01G24640 vs. NCBI nr
Match: KAE8653299.1 (hypothetical protein Csa_023327 [Cucumis sativus])

HSP 1 Score: 171.4 bits (433), Expect = 3.9e-39
Identity = 81/83 (97.59%), Postives = 81/83 (97.59%), Query Frame = 0


Query: 1  MDLSMALGHGTSSPPFSNRGATTSPPSTWLPPGVDPIQPKTISSAFQYFHPLMEFLAGLS 60
          MDLSM LGHGTSSPPFSNRG TTSPPSTWLPPGVDPIQPKTISSAFQYFHPLMEFLAGLS
Sbjct: 1  MDLSMGLGHGTSSPPFSNRGDTTSPPSTWLPPGVDPIQPKTISSAFQYFHPLMEFLAGLS 60

Query: 61 SDERVVLIGHSFGGNGGFPREDL 84
          SDERVVLIGHSFGGNGGFPREDL
Sbjct: 61 SDERVVLIGHSFGGNGGFPREDL 83

[Close]

BLAST of CSPI01G24640 vs. NCBI nr
Match: TYJ96886.1 (salicylic acid-binding protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 85.5 bits (210), Expect = 2.8e-13
Identity = 38/44 (86.36%), Postives = 41/44 (93.18%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           G+DPIQPKT+SSAFQYFHPL EFLAGL SDERVVL+GHSFGG G
Sbjct: 151 GIDPIQPKTVSSAFQYFHPLTEFLAGLPSDERVVLVGHSFGGIG 194

[Close]

BLAST of CSPI01G24640 vs. NCBI nr
Match: XP_008444767.1 (PREDICTED: salicylic acid-binding protein 2-like [Cucumis melo])

HSP 1 Score: 85.5 bits (210), Expect = 2.8e-13
Identity = 38/44 (86.36%), Postives = 41/44 (93.18%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           G+DPIQPKT+SSAFQYFHPL EFLAGL SDERVVL+GHSFGG G
Sbjct: 73  GIDPIQPKTVSSAFQYFHPLTEFLAGLPSDERVVLVGHSFGGIG 116

[Close]

BLAST of CSPI01G24640 vs. NCBI nr
Match: XP_023001470.1 (LOW QUALITY PROTEIN: putative inactive methylesterase 20 [Cucurbita maxima])

HSP 1 Score: 76.3 bits (186), Expect = 1.7e-10
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           GVDP QPKTISSAFQYF PL EFLAG  S ERVVL+GHSFGG G
Sbjct: 68  GVDPRQPKTISSAFQYFQPLTEFLAGFPSGERVVLVGHSFGGLG 111

[Close]

BLAST of CSPI01G24640 vs. NCBI nr
Match: KAG6583867.1 (Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 75.9 bits (185), Expect = 2.2e-10
Identity = 36/44 (81.82%), Postives = 37/44 (84.09%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           GVDP QPKTISSAF YF PL EFLAGL S ERVVL+GHSFGG G
Sbjct: 68  GVDPRQPKTISSAFHYFQPLTEFLAGLPSGERVVLVGHSFGGLG 111

[Close]

BLAST of CSPI01G24640 vs. TAIR 10
Match: AT5G58310.1 (methyl esterase 18 )

HSP 1 Score: 47.4 bits (111), Expect = 7.8e-06
Identity = 19/42 (45.24%), Postives = 28/42 (66.67%), Query Frame = 0


Query: 33 GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGG 75
          G++P  P T+SS   Y  PL  FL+ L +D++V+L+ HS GG
Sbjct: 42 GINPTDPNTVSSLDDYDEPLYAFLSQLPNDQKVILVSHSVGG 83

[Close]

BLAST of CSPI01G24640 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 47.0 bits (110), Expect = 1.0e-05
Identity = 22/43 (51.16%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 33 GVDPIQPKT-ISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGG 75
          G+D  +  T IS+  QY  PLM+ +  L +DE+VVL+GHSFGG
Sbjct: 46 GIDTTRSITDISTCEQYSEPLMQLMTSLPNDEKVVLVGHSFGG 88

[Close]

BLAST of CSPI01G24640 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 45.4 bits (106), Expect = 3.0e-05
Identity = 19/42 (45.24%), Postives = 28/42 (66.67%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGG 75
           G+D      IS+  QY  PL++ +  L +DE+VVL+GHS+GG
Sbjct: 64  GIDTRSITDISTCEQYSEPLIQLMTSLPNDEKVVLVGHSYGG 105

[Close]

BLAST of CSPI01G24640 vs. TAIR 10
Match: AT3G50440.1 (methyl esterase 10 )

HSP 1 Score: 44.7 bits (104), Expect = 5.1e-05
Identity = 20/44 (45.45%), Postives = 26/44 (59.09%), Query Frame = 0


Query: 33  GVDPIQPKTISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGGNG 77
           GVD  Q   +     Y  PLM F+  L  +E+VVL+GHS+GG G
Sbjct: 71  GVDTRQLHEVRLVSAYLEPLMSFMESLPENEKVVLVGHSYGGIG 114

[Close]

BLAST of CSPI01G24640 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 43.9 bits (102), Expect = 8.7e-05
Identity = 21/43 (48.84%), Postives = 28/43 (65.12%), Query Frame = 0


Query: 33 GVDPIQPKT-ISSAFQYFHPLMEFLAGLSSDERVVLIGHSFGG 75
          G+D  +  T IS+  QY  PLM+ +  L  DE+VVL+GHS GG
Sbjct: 46 GIDMTRSITDISTCEQYSEPLMQLMTSLPDDEKVVLVGHSLGG 88

The following BLAST results are available for this feature:

BLAST of CSPI01G24640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVL9	1.1e-04	45.24	Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1	[more]
O80476	1.4e-04	51.16	Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1	[more]
O80475	4.2e-04	45.24	Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1	[more]
Q9SE93	4.2e-04	47.62	Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=...	[more]
Q8S9K8	7.1e-04	45.45	Methylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=MES10 PE=2 SV=1	[more]

BLAST of CSPI01G24640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BD36	1.3e-13	86.36	Salicylic acid-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3BAM5	1.3e-13	86.36	salicylic acid-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103488011 PE...	[more]
A0A6J1KLA2	8.2e-11	81.82	LOW QUALITY PROTEIN: putative inactive methylesterase 20 OS=Cucurbita maxima OX=...	[more]
A0A6J1CDF0	8.5e-08	70.45	salicylic acid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1CFI6	3.2e-07	68.18	methylesterase 3-like OS=Momordica charantia OX=3673 GN=LOC111010761 PE=4 SV=1	[more]

BLAST of CSPI01G24640 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8653299.1	3.9e-39	97.59	hypothetical protein Csa_023327 [Cucumis sativus]	[more]
TYJ96886.1	2.8e-13	86.36	salicylic acid-binding protein 2-like [Cucumis melo var. makuwa]	[more]
XP_008444767.1	2.8e-13	86.36	PREDICTED: salicylic acid-binding protein 2-like [Cucumis melo]	[more]
XP_023001470.1	1.7e-10	81.82	LOW QUALITY PROTEIN: putative inactive methylesterase 20 [Cucurbita maxima]	[more]
KAG6583867.1	2.2e-10	81.82	Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of CSPI01G24640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58310.1	7.8e-06	45.24	methyl esterase 18	[more]
AT2G23600.1	1.0e-05	51.16	acetone-cyanohydrin lyase	[more]
AT2G23590.1	3.0e-05	45.24	methyl esterase 8	[more]
AT3G50440.1	5.1e-05	45.45	methyl esterase 10	[more]
AT2G23610.1	8.7e-05	48.84	methyl esterase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 5..77 e-value: 7.6E-6 score: 28.0
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 8..75
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 10..28
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..34

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G24640.1	CSPI01G24640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009694	jasmonic acid metabolic process
biological_process	GO:0009696	salicylic acid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0080030	methyl indole-3-acetate esterase activity
molecular_function	GO:0080032	methyl jasmonate esterase activity
molecular_function	GO:0080031	methyl salicylate esterase activity