CSPI01G23560 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G23560
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDNA-directed RNA polymerases I and III subunit RPAC2
LocationChr1: 19071835 .. 19075500 (+)
RNA-Seq ExpressionCSPI01G23560
SyntenyCSPI01G23560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAGTATTTTACAAAATTGTTACTTCTTGATTTTAGGTTTAAAGGTATACTTCCAATGTCACCAAAACTCAGGAACCGGATTTGGGACGGTTCGGTTCAAATAGACCATCTCAGCTTGGTATCGGTTCAAACGGACCAGCGTGACCGAATTATCAAATCAAAAGGGCCCAAGCCTTAAACCCCATTCAACCTCTTTTTCGTTTTTGTGATTTGTTCCCTCTCCCGGTGTCTCTCCTCCCCAACTCACCGGCCGGCCGGCCACCGACGGCAGCTACTTCATCTTCTCGCCCTTCTTCTCTCTCTTGGTAATCAGTTCTCTCTCTCTCTCATATATATATGTATGTATGTATGTGTGTGTGTATGTATGTATGGTCGAGACACCTATAAACAAGTGAGCAATGCGAATGTCTGTTGCTTATTCATGTGTGTGTGTAAGCAATAGCTGGGTGACATTGTTGAATTGACTGATTTTTGCAGTTTTAGTTCTGAAGAATCTAATGGTTATTTTTTTAATGTAATTTTCTTTGTGTTTTGGGCTACGTTAAGGTATGGTCCAGTTATTTTAGATAGTTGAATTTAGAATTTTTGGTGATGTCCGTGAAAACTCGTTTAGTCATGTCGCTAGGGAACTAAAGAATAGTGAATCGATCTGGAATTTTACTCATTAGTACTATCCTATAAGGGGAACTAGAATACATGAAAGAGGCTCAATGCTGAATGCCCAAAACCTGAAAAGTGAAGTTAATGTTGTATTAGTTTTGATTCACCATATCTGAATGTTATCGTGAAAGAGGTGATGATAAAATTGGAGTTAGCTTAAGATCTCCCAAGTAGTAAGATTGAAACAGACTTAATCATGCCAATTTGTTCCTTGGTACTTTTTAGATTAAAAAACCAATAGGTAGCCCTCTTGTAAAAGTGATTTCTTAAGGCGCCCTATGAAACTAGACATGTTACTCTAGCTCTTACATTCTGTTTAGAAAGTTGCAGTCAATTCTCCCCAAAAGTGTAATTGATAGGAAAGAATGAATTAGTAGTCATATATTTATTGCTCAATAGTGCTTTCTAATCTTCTTCATACTTAGACCTTAAACTTCAGTGGCTTCTCTTTCTAGCCTTTTAGTAGTAACAACATCCGTACAAGTAAAGCTTGTAAGGATAGGATAGTAATATAGTCTTGGCGAAATAACTGATCAAAAATTTCTTTTTCAAGCTACGTATATGTATGTGTTATAGATTTTTTAGGTTTGTGGGAATAATTGAAGAATCATGAAAGTAATCTAGCTAATCTTACATGCCTGCACTTCTGTTAATGCCCGAAGATTGATATTTCAATTCTTGAGAGTTGGTGGAAAAGAGATTTCAACTTCTTGGCTCCATTGGTTTTGTAGAATTTTGGCTTTTTTTTTCCTTATGTTATCAATTATTTGCTATTTTTGAGAATTTTGGTTTATTTTCCTTGATCTTCTCAATTTATTTGCCTTCATTTTTAATCAAGATGTTGGTTTTGTCTGGTTTTTCTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTAGCAATGGAACATGGATCCTTTACAGATGAGACCAAATCAACATTTTCTATGGCTGATGAAGATCATACACTTGCAAATGCTCTCAGATGCACTTTGAATCAAGAGTAGGAGCTCTTATTCAGTTATTCTTAAGTGAATACTTTGAAATGTCCACGATCATTTTTTCTAACCATTTCATCATGGACATCTATTAATTCATGACATTAATAATTAAATAGATTCTTGGTTCTTGTGAACATATAATCAACAAGGTCCTTGAAGAATTGATTAGGTTCTTGGATAATCCAGGAGGCATAAATTGATAAATAATGTACAGCCATTGCATGTGTTAATATGGGTTCTTTTACCGTCTATTCAGAAGAGTTATAAATATGTTGACATCTTTTTCACTTATTCAAGCAATAGGAATTGATCATTTGGTTGTGGTTTCTTTCCCTGTTAAAAAAAACTGGTTTAGATTGTTGTTATACTTTAGTAAACCATATCTGACATACTTTTAAATAACTTGTAATATCATCCTGCAGTCCAAGAGTGACATTCTGTGGGTATAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTAGAGTCCAGACAACAGGTAATATTTTGAGGTTGTTTACTCTCTAATTGGTTCACATTTCAGAAATTTTTTTCTAATGATTGGATTTGTTCTATCTTGATTTTTCTTCTTTATACACATCTAATTTATGCATTCATGAGATATATAGCTTTTGGATCCATGCAATGAGTGATCCTTCTAAGAAATTTTTTTTCCAACAAAATCGCCTTTTTTTGCAGAACACATGTTGGATCCTTGTAGCTTTGCTAGAACCCCATTATTTTTATAATTGAATAGAACCCAAGGAAACAGCTGTACAACATATAGAATTGATTCTGTTCAAGTAAGGTCGTGGTAAAAAATAAATTTTACTACTTGAAATTAGGGTCTATCTATTTAGTTCTCTAACTTTAACAAGATTCAATTATTTGTCCAAATTTTGAATAGCTATTGTCCATAGTTCTCACCATCGACACTGGTAACTCACGTTGACATGATATATGTATTCGAAAAATATTAGTTGATTGGATGTAACATGACAGAAAAATAGTATGAAGTATATAACTATAGAAAATAATAGTAGCACATAAGAGGGATTTGATACGTTGCTTTGAAAAGATATTATGTTGCATATGCTTGTTTATTTTATTCAATTGTCCCCTTTTTTCCACATTACAATGTTAGTAAGTACGACTATACGTGATTAATTACACTTCAAAGTTTGAAAGCCATGTTTAAAACTTTTTAAAATGATAATACCTATTGGGCACAACTATGAAGTTACACTAGAACACAGTTGTACAACCTAGAGAATAGATCCTATTCAAGTAGGGTAATAGATCTATAATTTAATCTCAAACATATATGCATAAGCATACCCATTTGCAATTGTAAAGTAAATCCCAAGGTACCAAGATGTTTGAGGCTGAGGCCTAAAAAATAGTGTCCCAAATGAATGGTCTTTACTCTTTCTCCATGGCTGGATTTGTTGAACCCAGTGATCTGCAGTTGAAAGTAGACAAAGCTATTATTGATTACTAGGGAAAAGCAAAGCACATGGGAAAATGGTAGGGGCAATATAGCAAACAGAAGGCTATTTGTTGAAGTGACTATGCATTATTAGTAGCATTAACAAATAGCATTAGAGATCACTGATGAAAGGGGTTTTTGATTGAACATGGCAGGTGATCCTGCTAGAGAAGTTTTGAAAGATGCTTGTCAGAACTTGATGGTGGTGTGTCAGCATGTGAGGAACACATTTGACAAGGCTGTGCTTGATTTCAATCTCACCAAACCAAAAGAAAAACCTGTGGGAGACATTAATATCAAATAGAATGGGACCTTTGTTTTTTCTTTGCCTTATAATTTTACCCTTTTTTTTGTTAGTTTACAGTTTATTGAGATGGGTTAGTTAGAAACAGATTAATTCCATTGAGTTTTGCTTGTTTTTATTGGTATCTCAACTGATGGCTTATCCCTCCCTTCAGGGATTTCATGTTAAATATTAGGTTTGGTTGATTTTACATTTAATTAGAAGAATGC

mRNA sequence

CGAGTATTTTACAAAATTGTTACTTCTTGATTTTAGGTTTAAAGGTATACTTCCAATGTCACCAAAACTCAGGAACCGGATTTGGGACGGTTCGGTTCAAATAGACCATCTCAGCTTGGTATCGGTTCAAACGGACCAGCGTGACCGAATTATCAAATCAAAAGGGCCCAAGCCTTAAACCCCATTCAACCTCTTTTTCGTTTTTGTGATTTGTTCCCTCTCCCGGTGTCTCTCCTCCCCAACTCACCGGCCGGCCGGCCACCGACGGCAGCTACTTCATCTTCTCGCCCTTCTTCTCTCTCTTGCAATGGAACATGGATCCTTTACAGATGAGACCAAATCAACATTTTCTATGGCTGATGAAGATCATACACTTGCAAATGCTCTCAGATGCACTTTGAATCAAGATCCAAGAGTGACATTCTGTGGGTATAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTAGAGTCCAGACAACAGGTGATCCTGCTAGAGAAGTTTTGAAAGATGCTTGTCAGAACTTGATGGTGGTGTGTCAGCATGTGAGGAACACATTTGACAAGGCTGTGCTTGATTTCAATCTCACCAAACCAAAAGAAAAACCTGTGGGAGACATTAATATCAAATAGAATGGGACCTTTGTTTTTTCTTTGCCTTATAATTTTACCCTTTTTTTTGTTAGTTTACAGTTTATTGAGATGGGTTAGTTAGAAACAGATTAATTCCATTGAGTTTTGCTTGTTTTTATTGGTATCTCAACTGATGGCTTATCCCTCCCTTCAGGGATTTCATGTTAAATATTAGGTTTGGTTGATTTTACATTTAATTAGAAGAATGC

Coding sequence (CDS)

ATGGAACATGGATCCTTTACAGATGAGACCAAATCAACATTTTCTATGGCTGATGAAGATCATACACTTGCAAATGCTCTCAGATGCACTTTGAATCAAGATCCAAGAGTGACATTCTGTGGGTATAGCATCCCTCATCCTTCAGATAATCGAGTTAATATTAGAGTCCAGACAACAGGTGATCCTGCTAGAGAAGTTTTGAAAGATGCTTGTCAGAACTTGATGGTGGTGTGTCAGCATGTGAGGAACACATTTGACAAGGCTGTGCTTGATTTCAATCTCACCAAACCAAAAGAAAAACCTGTGGGAGACATTAATATCAAATAG

Protein sequence

MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK*
Homology
[Close]
BLAST of CSPI01G23560 vs. ExPASy Swiss-Prot
Match: Q5XK67 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Xenopus laevis OX=8355 GN=polr1d PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 3.1e-16
Identity = 40/90 (44.44%), Postives = 59/90 (65.56%), Query Frame = 0 

Query: 8   DETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQT-TGDPAREV 67
           +E+  TF + +EDHTL N+LR  + ++P V FCGYSI HPS+ ++N R+QT  G PA E 
Sbjct: 20  EESCVTFVLHEEDHTLGNSLRYMVMKNPEVEFCGYSITHPSETKINFRIQTRNGIPAVEP 79

Query: 68  LKDACQNLMVVCQHVRNTFDKAVLDFNLTK 97
            +     L+ VCQHV NTF+ ++ ++   K
Sbjct: 80  FRRGLNELVDVCQHVLNTFETSIKEYKEQK 109

[Close]
BLAST of CSPI01G23560 vs. ExPASy Swiss-Prot
Match: P97304 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Mus musculus OX=10090 GN=Polr1d PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.5e-15
Identity = 40/101 (39.60%), Postives = 60/101 (59.41%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           M   + TD    TF + +EDHTL N LR  + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRQCVTFVLHEEDHTLGNCLRYIIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 61  D-PAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEK 101
             PA E  +     L+ VCQHV   F+ ++ D+   K  +K
Sbjct: 89  ALPAVEPFQKGLNELLNVCQHVLVKFEASIKDYKAKKASKK 129

[Close]
BLAST of CSPI01G23560 vs. ExPASy Swiss-Prot
Match: P0DPB6 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Homo sapiens OX=9606 GN=POLR1D PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.0e-15
Identity = 39/93 (41.94%), Postives = 59/93 (63.44%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           M   + TD    TF + +EDHTL N+LR  + ++P V FCGY+  HPS++++N+R+QT G
Sbjct: 29  MVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSESKINLRIQTRG 88

Query: 61  D-PAREVLKDACQNLMVVCQHVRNTFDKAVLDF 93
             PA E  +     LM VCQHV + F+ ++ D+
Sbjct: 89  TLPAVEPFQRGLNELMNVCQHVLDKFEASIKDY 121

[Close]
BLAST of CSPI01G23560 vs. ExPASy Swiss-Prot
Match: Q6DRI4 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Danio rerio OX=7955 GN=polr1d PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 2.6e-15
Identity = 40/86 (46.51%), Postives = 56/86 (65.12%), Query Frame = 0 

Query: 8   DETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQT-TGDPAREV 67
           DE   TF + +EDHTL N+LR  + +   V FCGYSI HPS++++N R+QT  G PA E 
Sbjct: 19  DEGCVTFVLNEEDHTLGNSLRYMIMKSQDVEFCGYSITHPSESKINFRIQTRDGVPASEP 78

Query: 68  LKDACQNLMVVCQHVRNTFDKAVLDF 93
           L++   NL  VC+HV  TF+  + +F
Sbjct: 79  LRNGLNNLTDVCKHVLQTFEARMKEF 104

[Close]
BLAST of CSPI01G23560 vs. ExPASy Swiss-Prot
Match: P28000 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPC19 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 4.5e-15
Identity = 38/83 (45.78%), Postives = 57/83 (68.67%), Query Frame = 0 

Query: 8   DETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTGD-PAREV 67
           D T ++F + +EDHTL NALR  + ++P V FCGYSIPHPS+N +NIR+QT G+  A + 
Sbjct: 57  DGTSASFQIVEEDHTLGNALRYVIMKNPDVEFCGYSIPHPSENLLNIRIQTYGETTAVDA 116

Query: 68  LKDACQNLMVVCQHVRNTFDKAV 90
           L+   ++LM +C  V + F + +
Sbjct: 117 LQKGLKDLMDLCDVVESKFTEKI 139

[Close]
BLAST of CSPI01G23560 vs. ExPASy TrEMBL
Match: A0A0A0LV21 (RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G527910 PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.1e-56
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 108

[Close]
BLAST of CSPI01G23560 vs. ExPASy TrEMBL
Match: A0A1S3C5G1 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo OX=3656 GN=LOC103497108 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.4e-53
Identity = 103/108 (95.37%), Postives = 105/108 (97.22%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALR TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTK KE+P GDINI+
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKAKEEPEGDINIE 108

[Close]
BLAST of CSPI01G23560 vs. ExPASy TrEMBL
Match: A0A5D3BBZ7 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001620 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.4e-53
Identity = 103/108 (95.37%), Postives = 105/108 (97.22%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALR TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTK KE+P GDINI+
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKAKEEPEGDINIE 108

[Close]
BLAST of CSPI01G23560 vs. ExPASy TrEMBL
Match: A0A5A7SR68 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00190 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.8e-52
Identity = 102/108 (94.44%), Postives = 105/108 (97.22%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALR TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTK KE+P GDI+I+
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKAKEEPEGDISIE 108

[Close]
BLAST of CSPI01G23560 vs. ExPASy TrEMBL
Match: A0A6J1D9Q8 (DNA-directed RNA polymerases I and III subunit RPAC2 OS=Momordica charantia OX=3673 GN=LOC111018936 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.1e-48
Identity = 97/108 (89.81%), Postives = 101/108 (93.52%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSF+DE+KSTFSMADEDHTLANALR TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFSDESKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           D AREVLKD+CQNLMVVCQHVRNTFDK+VLDF LT    KPVGDINIK
Sbjct: 61  DSAREVLKDSCQNLMVVCQHVRNTFDKSVLDFKLT----KPVGDINIK 104

[Close]
BLAST of CSPI01G23560 vs. NCBI nr
Match: XP_004145267.1 (DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis sativus] >KGN65775.1 hypothetical protein Csa_023153 [Cucumis sativus])

HSP 1 Score: 228.8 bits (582), Expect = 2.3e-56
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 108

[Close]
BLAST of CSPI01G23560 vs. NCBI nr
Match: XP_008457416.1 (PREDICTED: DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis melo] >XP_008457417.1 PREDICTED: DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis melo] >TYJ97352.1 DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis melo var. makuwa])

HSP 1 Score: 216.1 bits (549), Expect = 1.5e-52
Identity = 103/108 (95.37%), Postives = 105/108 (97.22%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALR TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTK KE+P GDINI+
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKAKEEPEGDINIE 108

[Close]
BLAST of CSPI01G23560 vs. NCBI nr
Match: KAA0031779.1 (DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis melo var. makuwa])

HSP 1 Score: 214.2 bits (544), Expect = 5.8e-52
Identity = 102/108 (94.44%), Postives = 105/108 (97.22%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALR TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTK KE+P GDI+I+
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKAKEEPEGDISIE 108

[Close]
BLAST of CSPI01G23560 vs. NCBI nr
Match: XP_038893936.1 (DNA-directed RNA polymerases I and III subunit RPAC2 [Benincasa hispida])

HSP 1 Score: 205.7 bits (522), Expect = 2.1e-49
Identity = 101/108 (93.52%), Postives = 101/108 (93.52%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLANALR TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANALRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLMVVCQHVRNTFDKAVLD  LT    KPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDIKLT----KPVGDINIK 104

[Close]
BLAST of CSPI01G23560 vs. NCBI nr
Match: XP_023522054.1 (DNA-directed RNA polymerases I and III subunit RPAC2-like [Cucurbita pepo subsp. pepo] >XP_023524948.1 DNA-directed RNA polymerases I and III subunit RPAC2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 199.5 bits (506), Expect = 1.5e-47
Identity = 97/108 (89.81%), Postives = 99/108 (91.67%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFTDETKSTFSMADEDHTLAN++R TLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG
Sbjct: 1   MEHGSFTDETKSTFSMADEDHTLANSIRYTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREVLKDACQNLM VCQHVRNT DKAVLD  LT    KPVGDINIK
Sbjct: 61  DPAREVLKDACQNLMAVCQHVRNTLDKAVLDHKLT----KPVGDINIK 104

[Close]
BLAST of CSPI01G23560 vs. TAIR 10
Match: AT2G29540.1 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 134.4 bits (337), Expect = 5.4e-32
Identity = 62/108 (57.41%), Postives = 84/108 (77.78%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFT+ + ++F++++EDHTLANA+R  LNQDPRVT   Y+IPHPS  +VNIRVQTTG
Sbjct: 1   MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREV KDACQ LM + +HVR+ FDKAV ++   + +++   +  +K
Sbjct: 61  DPAREVFKDACQELMQMNRHVRSVFDKAVAEYKDEQKRKEEAEEEELK 108

[Close]
BLAST of CSPI01G23560 vs. TAIR 10
Match: AT2G29540.2 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 134.4 bits (337), Expect = 5.4e-32
Identity = 62/108 (57.41%), Postives = 84/108 (77.78%), Query Frame = 0 

Query: 1   MEHGSFTDETKSTFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 60
           MEHGSFT+ + ++F++++EDHTLANA+R  LNQDPRVT   Y+IPHPS  +VNIRVQTTG
Sbjct: 1   MEHGSFTNVSHASFTLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTTG 60

Query: 61  DPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNLTKPKEKPVGDINIK 109
           DPAREV KDACQ LM + +HVR+ FDKAV ++   + +++   +  +K
Sbjct: 61  DPAREVFKDACQELMQMNRHVRSVFDKAVAEYKDEQKRKEEAEEEELK 108

[Close]
BLAST of CSPI01G23560 vs. TAIR 10
Match: AT2G29540.3 (RNApolymerase 14 kDa subunit )

HSP 1 Score: 89.7 bits (221), Expect = 1.5e-18
Identity = 41/74 (55.41%), Postives = 54/74 (72.97%), Query Frame = 0 

Query: 35  PRVTFCGYSIPHPSDNRVNIRVQTTGDPAREVLKDACQNLMVVCQHVRNTFDKAVLDFNL 94
           PRVT   Y+IPHPS  +VNIRVQTTGDPAREV KDACQ LM + +HVR+ FDKAV ++  
Sbjct: 52  PRVTVAAYTIPHPSLEQVNIRVQTTGDPAREVFKDACQELMQMNRHVRSVFDKAVAEYKD 111

Query: 95  TKPKEKPVGDINIK 109
            + +++   +  +K
Sbjct: 112 EQKRKEEAEEEELK 125

[Close]
BLAST of CSPI01G23560 vs. TAIR 10
Match: AT3G52090.1 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 55.8 bits (133), Expect = 2.4e-08
Identity = 28/94 (29.79%), Postives = 51/94 (54.26%), Query Frame = 0 

Query: 5   SFTDETK----STFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 64
           S+  +TK    ++F++  EDHT+ N +R  L++D  V F GY +PHP   ++ +R+ TT 
Sbjct: 20  SYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVRIHTTS 79

Query: 65  DPA-REVLKDACQNLMVVCQHVRNTFDKAVLDFN 94
             +  +    A  +L     +++N F+  V  F+
Sbjct: 80  QSSPMQAYNQAINDLDKELDYLKNQFEAEVAKFS 113

[Close]
BLAST of CSPI01G23560 vs. TAIR 10
Match: AT3G52090.2 (DNA-directed RNA polymerase, RBP11-like )

HSP 1 Score: 47.0 bits (110), Expect = 1.1e-05
Identity = 28/77 (36.36%), Postives = 43/77 (55.84%), Query Frame = 0 

Query: 5  SFTDETK----STFSMADEDHTLANALRCTLNQDPRVTFCGYSIPHPSDNRVNIRVQTTG 64
          S+  +TK    ++F++  EDHT+ N +R  L++D  V F GY +PHP   ++ +R     
Sbjct: 20 SYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAGYQLPHPLKYKIIVR----- 79

Query: 65 DPAREVLKDACQNLMVV 78
          DP     KD  +NL VV
Sbjct: 80 DP-----KDWFRNLKVV 86

The following BLAST results are available for this feature:
BLAST of CSPI01G23560 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q5XK673.1e-1644.44DNA-directed RNA polymerases I and III subunit RPAC2 OS=Xenopus laevis OX=8355 G... [more]
P973041.5e-1539.60DNA-directed RNA polymerases I and III subunit RPAC2 OS=Mus musculus OX=10090 GN... [more]
P0DPB62.0e-1541.94DNA-directed RNA polymerases I and III subunit RPAC2 OS=Homo sapiens OX=9606 GN=... [more]
Q6DRI42.6e-1546.51DNA-directed RNA polymerases I and III subunit RPAC2 OS=Danio rerio OX=7955 GN=p... [more]
P280004.5e-1545.78DNA-directed RNA polymerases I and III subunit RPAC2 OS=Saccharomyces cerevisiae... [more]
BLAST of CSPI01G23560 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LV211.1e-56100.00RNA_pol_L_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G527910... [more]
A0A1S3C5G17.4e-5395.37DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo OX=3656 GN=... [more]
A0A5D3BBZ77.4e-5395.37DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo var. makuwa... [more]
A0A5A7SR682.8e-5294.44DNA-directed RNA polymerases I and III subunit RPAC2 OS=Cucumis melo var. makuwa... [more]
A0A6J1D9Q87.1e-4889.81DNA-directed RNA polymerases I and III subunit RPAC2 OS=Momordica charantia OX=3... [more]
BLAST of CSPI01G23560 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004145267.12.3e-56100.00DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis sativus] >KGN65775... [more]
XP_008457416.11.5e-5295.37PREDICTED: DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis melo] >... [more]
KAA0031779.15.8e-5294.44DNA-directed RNA polymerases I and III subunit RPAC2 [Cucumis melo var. makuwa][more]
XP_038893936.12.1e-4993.52DNA-directed RNA polymerases I and III subunit RPAC2 [Benincasa hispida][more]
XP_023522054.11.5e-4789.81DNA-directed RNA polymerases I and III subunit RPAC2-like [Cucurbita pepo subsp.... [more]
BLAST of CSPI01G23560 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G29540.15.4e-3257.41RNApolymerase 14 kDa subunit [more]
AT2G29540.25.4e-3257.41RNApolymerase 14 kDa subunit [more]
AT2G29540.31.5e-1855.41RNApolymerase 14 kDa subunit [more]
AT3G52090.12.4e-0829.79DNA-directed RNA polymerase, RBP11-like [more]
AT3G52090.21.1e-0536.36DNA-directed RNA polymerase, RBP11-like [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009025DNA-directed RNA polymerase, RBP11-like dimerisation domainPFAMPF13656RNA_pol_L_2coord: 12..83
e-value: 1.2E-28
score: 98.7
IPR036603RNA polymerase, RBP11-like subunitGENE3D3.30.1360.10coord: 2..94
e-value: 1.1E-33
score: 117.3
IPR036603RNA polymerase, RBP11-like subunitSUPERFAMILY55257RBP11-like subunits of RNA polymerasecoord: 8..92
NoneNo IPR availablePANTHERPTHR13946:SF37SUBFAMILY NOT NAMEDcoord: 1..97
NoneNo IPR availablePANTHERPTHR13946DNA-DIRECTED RNA POLYMERASE I,II,IIIcoord: 1..97
IPR008193DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved sitePROSITEPS01154RNA_POL_L_13KDcoord: 16..47
IPR033898DNA-directed RNA polymerases I and III subunit AC19CDDcd07029RNAP_I_III_AC19coord: 2..85
e-value: 8.10564E-43
score: 133.126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G23560.1CSPI01G23560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0046983 protein dimerization activity