CSPI01G23350 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G23350
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	Chr1: 18955574 .. 18959239 (+)
RNA-Seq Expression	CSPI01G23350
Synteny	CSPI01G23350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTGACACATTTGGTAGTGTGGTTATTAGATTAGTCTAACACTAAAATTTGATTGTCCACGAACTATTTTCCATTGTGAGTTGCTGTGATTTTATAAACTGAACTTCTTATTTGAGTGTTTTTTTCCATTGTTAAGAAAAAGCTTGTATAATATTTAGCACAGCAAATCGCATGGTAGCAAAACCTGCAATCTGTTGAGGCCATCCCCCAAAGCGAGAGGGCAGAAGAATGTTATTGGTTCTTGTTTCTCCTTCATCCTTTCCATCATTCTCTTCTTCTACCTTCAACAAATCCCCTTTCTCAACATTCTCTTCATTTCCCATTTTGTGCTCATTCAACTCTTTTTGCCTTTTCCCATCACATTCTACTCACAAACTCATCAAGCGCCTGAACCCCATTAGAAATCAAACTGAATCAGACTTCAATAGCAAATATGGTCGTACCCCAGTTGACGATCGGTGGTCTCTTCGCGGAAAGACGGCTCTCGTTACCGGTGGCACTCGTGGAATCGGGTAAATCAATCTGTATCAACGAATAGAGATTTTCATTTTTCTTTGTTGCATTTACTGTTTCTTTCCTCTGTTTTATTGAGAATTTGGAATTGGGTTTTGGTCTAGGCGTGCGATTGTGGAGGAATTAGTCGGTTTTGGGGCGAGAGTGCATACTTGTTCTCGGAATGAAGGTGAGTTGAGACAGTGTTTGAGGCATTGGAAGGATTCGGAATTTGAGGTTTCTGGGTCAGTCTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGTTAATGGAGAATGCGGGTAATACTTTTGATGGGAAGCTCAACATTCTTGTAAGGTGCACCCTTTCTTGCGTTTTTCTCTGCTTTAGATTAAGATGCCATTTTTCTTTGTTGGGCTACTTTCCTTTCAATCACTCATCTTACTGACCTTAAATTGATGAAATTATTGGAAGGAACCTTCTAGCTGCCACTCTCATTAGAAGTCTAATAAGAACATCAATTTTCTTTTCTCTATTGTGCTTGCAATTGTTATAAAGGTGACAACATTGATTGTTATTCTCGATAAAAAGTATTTGGCTAAGAATAAGACCTTTTTGCAACTATGAATCATTGCAATCAACGGTCAAGGAAGCTATATGAAGTTTTTTTTTTTTCTTTTGATATCCGTGAGTGTCCAGGATAGTATTGGGCACCGGGCACCTCAATTGGCGCTTAATCCATTTGGATGTTAAGGAAACTCATAGGATATTAAATCTTAGGTAGATGACCAACATTGATTGAACTCGTCCTTTCTTAGCCCTTTATCAAACTCAATTTACTAAGAACATGTGGCCTAGGTCCAAGTCATAGGATGAAGAAAGCTTTTAGTTGACATGGAATTAAAGAAGTTTCCACATATAGGAGCACTTGAAGACAAGAGCAATTAGAGAAGGCCAAATTCTAGAGACAACAAGCCTTGGAAGCACATACGTGATACATCTTGCACATTTTGAACTATGCATTGGGATTTACTTTGAGGCTTTATACTTTTAGCTAAACTCCTTGTTAGGATTCTTAATGTGTTTTTAAGTACGTTTACGTTAGTCACTCACCCTCAAAGGCGAAAGATGAAGATTTTAGGTTTTAGCTATTAAGCATCTTGTCATTTTCATTATTTTTCTTTATTCCCTCTATTATTTATGCAAGTTAATACTAAGGGTTTGCTTTTGAGAGTTTATCATGTCTCGTTGTCGTTCACTTAAGGCTTTGTAAGGCCTGATTCATACTTGTAAAATTGTTTGATACCTTGATGATGACATGAATCTGAAAATTATCTTTTTTGCCCATTGTTGAGAGCATGCGTGTATGGGCAATTCTATACCTAGTTTTCAATTTTCATTTTCATTTAAAAACATAGTTCGACTACTTGGCCAATTTGTGGAGTGTTTCACACTTGAGAATAGGAACTTGTTTTCCTTGCTTCTTGGTATTGGACGTCTAAAGGCAACCCCATCCTAACTGGTCCTTTGAGCGCTTGTGAAGTATTTGTGTTACAAGGTCCACGCCAAAGGGTTTTAGATCAATTTATCAGTCTCTCTTGTTGTTTGGTATCAAAGCTTTCGATTTGTATCAACTATTTAAAATGGGTAATGCCAATGTCTTCAAAGACTACACCCTTTGTGGGATATTTTCTCAAGAAGAAATAAGGGTTAAGTCTTGAGACAGCATGACCCATAAGGTTCTTATATTAGATACATAAATCTGTTCTAGTCCAAAGAAGAGTAAGTAAGATTAAAAGGTTAACAATTTTAGTCTCAGCCTTGAGATGAGCGAGCTTAAGGTGTAAGCCTCAAAATCCTTGCAAAACATTAGATGAGGATGAGTCAATAGAGTGGTAGCTTTATTCAGTAGATGCTGATCATTTAGCTAAAACAATTTTAGTTTACTATTTCTTAAATAAATGAATTTCGCCTTTAAGTATTCTTCAATGAAATCCTTTGGTGGATCTTCACTTTTTAAATGTGTATGTTACTTGTATGTTATTTCCTACTGACATTTAATCCTTAGGATATTTGCTGCTTTTTGGCATATGGAATTTTTACTTCTCTTTTCATAATGTGAAGTTAACGCTGAGGTGATTTTAAATTAGTTACGAACTAATCTTCCAACATCTTATGGGAAAATCCAAAATTTAAGAATAAAAAAGAACATCGATTTTCATCATTCTTTTAATCCTTATGGACTATGAATACCATTTCTGCTATCATGTAGGTGAATAATGTGGGAAGAAATATACGAAAGCCAACAACAGAATTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTTGAATCAGTCTTTCATTTATCACAACTTGCTTATCCTCTTCTAAAATCATCTGGCGAGGGAAGCATTGTATTCATGTCCTCGATCTCAAGTTTTGTGTCTCTGAAGTCCATGTCTGTTCAGGGAGCGACCAAAGGTGATCAACTTTATCCGTTGTATTTAAATATTATAGATGCAAACATCAATTTCTTTCTTGGAATATTTCCTAGCAATTTGTGATGGTTCTTTTCAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAATGGGCAAAAGACAACATAAGGAGCAATGCAGTTGCCCCATGGTACATCAAAACATCAATGGTGGAACAGGTAAATCTCAACGCCATTATGTTTTAACCTGTGAAGAATCAATCATAATGTTCATCTTCTTGATCTTTCGCTATGTTTCCTTTTAGGTGCTGAGTAACAAAGCATATGAAGAAGAGGTATATTCCCGAACCCCGCTTCGACGCTTGGGCGAACCAAGTGAAGTATCATCCTTAGTTGCATTCTTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAAATAACATTATAAATGAACATCTTCTATTTATCCCACTCCCCTCTCGGATATGTCTAGTTCGAGTGTTGATTTTTCTTTCACTTGCAAGGATGACACTCGCAAACAATTGATTGAAGCTTTTTGTTTCTTTTTCTTCAAAATGTTATTATGCTGAATGGTTCATGATATTTGAAAGGAAAAATGAGTTCTATAGTTTGGGGAACATTGAGGTATGATAGGAACCATGACTACGGC

mRNA sequence

ATTTTGACACATTTGGTAGTGTGGTTATTAGATTAGTCTAACACTAAAATTTGATTGTCCACGAACTATTTTCCATTGTGAGTTGCTGTGATTTTATAAACTGAACTTCTTATTTGAGTGTTTTTTTCCATTGTTAAGAAAAAGCTTGTATAATATTTAGCACAGCAAATCGCATGGTAGCAAAACCTGCAATCTGTTGAGGCCATCCCCCAAAGCGAGAGGGCAGAAGAATGTTATTGGTTCTTGTTTCTCCTTCATCCTTTCCATCATTCTCTTCTTCTACCTTCAACAAATCCCCTTTCTCAACATTCTCTTCATTTCCCATTTTGTGCTCATTCAACTCTTTTTGCCTTTTCCCATCACATTCTACTCACAAACTCATCAAGCGCCTGAACCCCATTAGAAATCAAACTGAATCAGACTTCAATAGCAAATATGGTCGTACCCCAGTTGACGATCGGTGGTCTCTTCGCGGAAAGACGGCTCTCGTTACCGGTGGCACTCGTGGAATCGGGCGTGCGATTGTGGAGGAATTAGTCGGTTTTGGGGCGAGAGTGCATACTTGTTCTCGGAATGAAGGTGAGTTGAGACAGTGTTTGAGGCATTGGAAGGATTCGGAATTTGAGGTTTCTGGGTCAGTCTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGTTAATGGAGAATGCGGGTAATACTTTTGATGGGAAGCTCAACATTCTTGTGAATAATGTGGGAAGAAATATACGAAAGCCAACAACAGAATTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTTGAATCAGTCTTTCATTTATCACAACTTGCTTATCCTCTTCTAAAATCATCTGGCGAGGGAAGCATTGTATTCATGTCCTCGATCTCAAGTTTTGTGTCTCTGAAGTCCATGTCTGTTCAGGGAGCGACCAAAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAATGGGCAAAAGACAACATAAGGAGCAATGCAGTTGCCCCATGGTACATCAAAACATCAATGGTGGAACAGGTGCTGAGTAACAAAGCATATGAAGAAGAGGTATATTCCCGAACCCCGCTTCGACGCTTGGGCGAACCAAGTGAAGTATCATCCTTAGTTGCATTCTTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAAATAACATTATAAATGAACATCTTCTATTTATCCCACTCCCCTCTCGGATATGTCTAGTTCGAGTGTTGATTTTTCTTTCACTTGCAAGGATGACACTCGCAAACAATTGATTGAAGCTTTTTGTTTCTTTTTCTTCAAAATGTTATTATGCTGAATGGTTCATGATATTTGAAAGGAAAAATGAGTTCTATAGTTTGGGGAACATTGAGGTATGATAGGAACCATGACTACGGC

Coding sequence (CDS)

ATGTTATTGGTTCTTGTTTCTCCTTCATCCTTTCCATCATTCTCTTCTTCTACCTTCAACAAATCCCCTTTCTCAACATTCTCTTCATTTCCCATTTTGTGCTCATTCAACTCTTTTTGCCTTTTCCCATCACATTCTACTCACAAACTCATCAAGCGCCTGAACCCCATTAGAAATCAAACTGAATCAGACTTCAATAGCAAATATGGTCGTACCCCAGTTGACGATCGGTGGTCTCTTCGCGGAAAGACGGCTCTCGTTACCGGTGGCACTCGTGGAATCGGGCGTGCGATTGTGGAGGAATTAGTCGGTTTTGGGGCGAGAGTGCATACTTGTTCTCGGAATGAAGGTGAGTTGAGACAGTGTTTGAGGCATTGGAAGGATTCGGAATTTGAGGTTTCTGGGTCAGTCTGTGATGTGTCTGTTCGAGCTCAGAGAGAGGAGTTAATGGAGAATGCGGGTAATACTTTTGATGGGAAGCTCAACATTCTTGTGAATAATGTGGGAAGAAATATACGAAAGCCAACAACAGAATTCACAGATGAAGAATTTTCTTTTCTTATGAAAACCAATGTTGAATCAGTCTTTCATTTATCACAACTTGCTTATCCTCTTCTAAAATCATCTGGCGAGGGAAGCATTGTATTCATGTCCTCGATCTCAAGTTTTGTGTCTCTGAAGTCCATGTCTGTTCAGGGAGCGACCAAAGGAGCTATCAATCAACTCACAAAATATTTAGCTTGTGAATGGGCAAAAGACAACATAAGGAGCAATGCAGTTGCCCCATGGTACATCAAAACATCAATGGTGGAACAGGTGCTGAGTAACAAAGCATATGAAGAAGAGGTATATTCCCGAACCCCGCTTCGACGCTTGGGCGAACCAAGTGAAGTATCATCCTTAGTTGCATTCTTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTGTTAATGGCTTTGACCCAAAATAA

Protein sequence

MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQTESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYITGQIIGVDGGMSVNGFDPK*

Homology

[Close]

BLAST of CSPI01G23350 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 9.9e-106
Identity = 200/286 (69.93%), Postives = 220/286 (76.92%), Query Frame = 0


Query: 52  KRLNPIRNQTESDFNSKYG-----RTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFG 111
           KR+NP    + S     Y           +RWSL G +ALVTGGTRGIGRAIVEEL G G
Sbjct: 35  KRINPKHKSSSSSVFVPYASQSSIAITSKERWSLNGMSALVTGGTRGIGRAIVEELAGLG 94

Query: 112 ARVHTCSRNEGELRQCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVN 171
           A VHTC+RNE EL  CL  W  S F V+GSVCDVS R+QRE LME   + F+GKL+ILVN
Sbjct: 95  AEVHTCARNEYELENCLSDWNRSGFRVAGSVCDVSDRSQREALMETVSSVFEGKLHILVN 154

Query: 172 NVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSL 231
           NVG NIRKP  EFT  EFS LM TN ESVFHL QLAYPLL+ S  GS+VF+SS+S FVSL
Sbjct: 155 NVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSL 214

Query: 232 KSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSR 291
           K+MSVQ +TKGAINQLT+ LACEWAKDNIR NAVAPWYIKTSMVEQVLSNK Y EEVYS 
Sbjct: 215 KNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTSMVEQVLSNKEYLEEVYSV 274

Query: 292 TPLRRLGEPSEVSSLVAFFCLPASSYITGQIIGVDGGMSVNGFDPK 333
           TPL RLGEP EVSS VAF CLPASSYITGQI+ VDGGMS+NGF P+
Sbjct: 275 TPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSINGFFPR 320

[Close]

BLAST of CSPI01G23350 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.3e-99
Identity = 176/255 (69.02%), Postives = 208/255 (81.57%), Query Frame = 0


Query: 75  DDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEVS 134
           D RWSL GKTALVTGGTRGIGRA+VEEL  FGA+VHTCSRN+ EL  CL  WK +   VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 135 GSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVES 194
           GSVCD SVR QRE+L++ A + F GKLNIL+NNVG N+RKPT E++ EE++ +M TN+ES
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLES 123

Query: 195 VFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKDN 254
            FHLSQ+A+PLLK+SG GSIVF+SS++  V L S S+ GATKGA+NQLT+ LACEWA DN
Sbjct: 124 AFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDN 183

Query: 255 IRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYIT 314
           IR+N VAPWYIKTS+VE +L  K + E V SRTPL R+GEP EVSSLVAF CLPASSYIT
Sbjct: 184 IRTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYIT 243

Query: 315 GQIIGVDGGMSVNGF 330
           GQ+I VDGG +VNGF
Sbjct: 244 GQVISVDGGFTVNGF 258

[Close]

BLAST of CSPI01G23350 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.1e-87
Identity = 158/256 (61.72%), Postives = 202/256 (78.91%), Query Frame = 0


Query: 74  VDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEV 133
           +D RWSL+G  ALVTGGT+GIG A+VEEL   GARVHTC+R+E +L++ LR W++  F+V
Sbjct: 1   MDKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQV 60

Query: 134 SGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVE 193
           + S+CDVS+R QRE+LME   + F GKLNILVNNVG  + KPTTE+T EEFSFLM TN++
Sbjct: 61  TTSICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLD 120

Query: 194 SVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 253
           S FH+SQLA+PLLK+SG GSIV MSSI+  V +   S+ GATKGA+NQL + LACEWA D
Sbjct: 121 SAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASD 180

Query: 254 NIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYI 313
           NIR+NA+ PW I T ++  +LS +  ++E   RTP+ R+GE +EVS LVAF CLPA+SYI
Sbjct: 181 NIRTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYI 240

Query: 314 TGQIIGVDGGMSVNGF 330
           TGQ+I VDGG++VNGF
Sbjct: 241 TGQVICVDGGLTVNGF 256

[Close]

BLAST of CSPI01G23350 vs. ExPASy Swiss-Prot
Match: Q9ZW19 (Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.9e-85
Identity = 157/256 (61.33%), Postives = 198/256 (77.34%), Query Frame = 0


Query: 77  RWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEVSGS 136
           RWSL G TALVTGG++GIG A+VEEL   GAR+HTC+R+E +L++ LR W+   F+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 137 VCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVF 196
           VCDVS R +RE+LME     F+GKLNILVNNVG  I KPT + T E+FSF M TN+ES F
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLESAF 132

Query: 197 HLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIR 256
           HLSQLA+PLLK+SG GSIV +SS+S  V +   S+ G +KGA+NQL + LACEWA DNIR
Sbjct: 133 HLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDNIR 192

Query: 257 SNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYITGQ 316
           +N+V PW+I+T +V + LSN+ + +EV SR P+ R+GE +EVSSLVAF CLPA+SYITGQ
Sbjct: 193 TNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQ 252

Query: 317 IIGVDGGMSVNGFDPK 333
            I VDGG +VNGF  K
Sbjct: 253 TICVDGGFTVNGFSFK 268

[Close]

BLAST of CSPI01G23350 vs. ExPASy Swiss-Prot
Match: Q9ZW20 (Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g29370 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-84
Identity = 154/253 (60.87%), Postives = 197/253 (77.87%), Query Frame = 0


Query: 77  RWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEVSGS 136
           +WSL G TALVTGG++G+G+A+VEEL   GARVHTC+R+E +L++ LR W+    +V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 137 VCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVF 196
           VCDVS R QRE+LME   + F GKL+ILV NVG  + KPTTE T EEFSF++ TN+ES F
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVGIGVLKPTTECTAEEFSFIIATNLESTF 132

Query: 197 HLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIR 256
           H SQLA+PLLK+SG G+IV MSS++  V+L + S+ GATKGA+NQL + LACEWA DNIR
Sbjct: 133 HFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDNIR 192

Query: 257 SNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYITGQ 316
           +N+V PW+I T   +  L +K  +E+V S TPLRR+GE +EVSSLVAF CLPA+SYITGQ
Sbjct: 193 ANSVCPWFITTPSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASYITGQ 252

Query: 317 IIGVDGGMSVNGF 330
            I VDGG ++NGF
Sbjct: 253 TICVDGGFTINGF 265

[Close]

BLAST of CSPI01G23350 vs. ExPASy TrEMBL
Match: A0A5D3BGS1 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001920 PE=4 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.2e-176
Identity = 319/332 (96.08%), Postives = 320/332 (96.39%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60
           MLL LVSPSSFPSFSSSTFNKS FSTFSSFPILCS N FCL PSHSTHKL KRLNPIRNQ
Sbjct: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60

Query: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDF SKYG TPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKD EFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSS+SSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK Y EEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFFCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAF CLPASSYITGQIIGVDGGMSVNGFDPK
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

[Close]

BLAST of CSPI01G23350 vs. ExPASy TrEMBL
Match: A0A1S3C5I5 (tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE=4 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.2e-176
Identity = 319/332 (96.08%), Postives = 320/332 (96.39%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60
           MLL LVSPSSFPSFSSSTFNKS FSTFSSFPILCS N FCL PSHSTHKL KRLNPIRNQ
Sbjct: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60

Query: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDF SKYG TPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKD EFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSS+SSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK Y EEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFFCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAF CLPASSYITGQIIGVDGGMSVNGFDPK
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

[Close]

BLAST of CSPI01G23350 vs. ExPASy TrEMBL
Match: A0A6J1KAL5 (tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111493193 PE=4 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.0e-150
Identity = 284/333 (85.29%), Postives = 295/333 (88.59%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSP--FSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIR 60
           M L LVSPS F S SSSTFN S    STFSS PI  S   F L  S+STHKL+KRL  IR
Sbjct: 1   MFLALVSPSPFDSLSSSTFNPSQPHRSTFSSSPISRSLKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120
           NQ ESDF +K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQAESDFKAKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNESE 120

Query: 121 LRQCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180
           L+QCLR WKD EFEVSGSVCDVSVRAQREELM+   +TF+GKLNILVNNVGRNIRKPTTE
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFNGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTN+ESVFHLSQLA+P LK+SGEGSIVFMSS+SSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNIESVFHLSQLAHPFLKASGEGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAY EEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFFCLPASSYITGQIIGVDGGMSVNGFDP 332
           SSLVAF CLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

[Close]

BLAST of CSPI01G23350 vs. ExPASy TrEMBL
Match: A0A6J1GAE7 (tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC111452252 PE=4 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.0e-149
Identity = 283/333 (84.98%), Postives = 293/333 (87.99%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSP--FSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIR 60
           M L LVSPS F S SSSTF+ S    STFSS PI  SF  F L  S+STHKL+KRL  IR
Sbjct: 1   MFLALVSPSPFDSLSSSTFSPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120
           NQ  SDF +K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQAASDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 121 LRQCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180
           L+QCLR WKD EFEVSGSVCDVSVRAQREELM+   +TFDGKLNILVNNVGRNIRKPTTE
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLA+P LK+SG GSIVFMSS+SSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWA DNIRSNAVAPWYIKTSMVEQVLSNKAY EEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFFCLPASSYITGQIIGVDGGMSVNGFDP 332
           SSLVAF CLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

[Close]

BLAST of CSPI01G23350 vs. ExPASy TrEMBL
Match: A0A6J1CL23 (tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC111012129 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.2e-147
Identity = 275/332 (82.83%), Postives = 288/332 (86.75%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60
           MLL LVSP+SF SFSS TF        S   I CSFN F L PS+ST K++ RL PI NQ
Sbjct: 1   MLLALVSPTSFDSFSSFTFITPTNPPLSPISISCSFNPFGLPPSYSTRKILHRLKPITNQ 60

Query: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           T+ D  S  G TP+DDRWSLRG TALVTGGTRGIGRAIVEELVGFGARVHTCSRN  EL+
Sbjct: 61  TQPDLKSMNGTTPIDDRWSLRGATALVTGGTRGIGRAIVEELVGFGARVHTCSRNLSELQ 120

Query: 121 QCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLR WK+ EF VSGSVCDVSVRAQREELME     FDGKLNILVNNVGRNIRKPT EFT
Sbjct: 121 QCLRDWKNLEFGVSGSVCDVSVRAQREELMETVAANFDGKLNILVNNVGRNIRKPTAEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAIN 240
           +EEFSFLMKTNVESVFHLSQLAYPLLK+SG GSIVFMSS+SSFVSLKSMSVQGATKGAIN
Sbjct: 181 EEEFSFLMKTNVESVFHLSQLAYPLLKASGRGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK Y EEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFFCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAF CLPASSYITGQIIGVDGGMS+NGFDP+
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSINGFDPR 332

[Close]

BLAST of CSPI01G23350 vs. NCBI nr
Match: XP_031741141.1 (tropinone reductase homolog At5g06060 [Cucumis sativus])

HSP 1 Score: 651.0 bits (1678), Expect = 5.6e-183
Identity = 329/332 (99.10%), Postives = 330/332 (99.40%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60
           MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ
Sbjct: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60

Query: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKD EFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSS+SSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFFCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAF CLPASSYITGQIIGVDGGMSVNGFDPK
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

[Close]

BLAST of CSPI01G23350 vs. NCBI nr
Match: XP_008457446.1 (PREDICTED: tropinone reductase homolog At5g06060 [Cucumis melo] >TYJ97378.1 tropinone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 627.5 bits (1617), Expect = 6.7e-176
Identity = 319/332 (96.08%), Postives = 320/332 (96.39%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60
           MLL LVSPSSFPSFSSSTFNKS FSTFSSFPILCS N FCL PSHSTHKL KRLNPIRNQ
Sbjct: 1   MLLALVSPSSFPSFSSSTFNKSHFSTFSSFPILCSSNPFCLSPSHSTHKLTKRLNPIRNQ 60

Query: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDF SKYG TPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFKSKYGHTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKD EFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSS+SSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSS 300
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK Y EEVYSRTPLRRLGEPSEVSS
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSS 300

Query: 301 LVAFFCLPASSYITGQIIGVDGGMSVNGFDPK 333
           LVAF CLPASSYITGQIIGVDGGMSVNGFDPK
Sbjct: 301 LVAFLCLPASSYITGQIIGVDGGMSVNGFDPK 332

[Close]

BLAST of CSPI01G23350 vs. NCBI nr
Match: XP_038893799.1 (tropinone reductase homolog At5g06060-like [Benincasa hispida])

HSP 1 Score: 577.4 bits (1487), Expect = 7.9e-161
Identity = 297/335 (88.66%), Postives = 308/335 (91.94%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNK---SPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPI 60
           MLL LVSPSSF SFSSSTFN+    P STFSSFPI CSF  F L PSHS+HKL+KRL PI
Sbjct: 1   MLLALVSPSSFYSFSSSTFNRPTNPPLSTFSSFPISCSFKPFWLSPSHSSHKLVKRLKPI 60

Query: 61  RNQTESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEG 120
            NQTESDF  KYG TPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEG
Sbjct: 61  TNQTESDFKIKYGSTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEG 120

Query: 121 ELRQCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTT 180
           ELRQCLR W + +FEVSGSVCDVSVRAQR+ELME   +TFDGKL+ILVNNVGRNIRKPTT
Sbjct: 121 ELRQCLRDWNNLKFEVSGSVCDVSVRAQRDELMEIVADTFDGKLSILVNNVGRNIRKPTT 180

Query: 181 EFTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKG 240
           EFTDEEFSFLMKTNVE+VFHLSQLAYPLLKSSGEGSIVFMSS+SS VSLKSMSVQGATKG
Sbjct: 181 EFTDEEFSFLMKTNVEAVFHLSQLAYPLLKSSGEGSIVFMSSVSSLVSLKSMSVQGATKG 240

Query: 241 AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSE 300
           AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNK Y EEVYSRTPLRRLGEPSE
Sbjct: 241 AINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKTYVEEVYSRTPLRRLGEPSE 300

Query: 301 VSSLVAFFCLPASSYITGQIIGVDGGMSVNGFDPK 333
           VSSLVAF CLPASSYITGQIIGVDGGMSVNGFDP+
Sbjct: 301 VSSLVAFLCLPASSYITGQIIGVDGGMSVNGFDPR 335

[Close]

BLAST of CSPI01G23350 vs. NCBI nr
Match: KAE8653260.1 (hypothetical protein Csa_019809 [Cucumis sativus])

HSP 1 Score: 554.3 bits (1427), Expect = 7.2e-154
Identity = 281/283 (99.29%), Postives = 282/283 (99.65%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60
           MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ
Sbjct: 1   MLLVLVSPSSFPSFSSSTFNKSPFSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIRNQ 60

Query: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120
           TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR
Sbjct: 61  TESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELR 120

Query: 121 QCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180
           QCLRHWKD EFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT
Sbjct: 121 QCLRHWKDLEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFT 180

Query: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAIN 240
           DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSS+SSFVSLKSMSVQGATKGAIN
Sbjct: 181 DEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSVSSFVSLKSMSVQGATKGAIN 240

Query: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEV 284
           QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEV
Sbjct: 241 QLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEV 283

[Close]

BLAST of CSPI01G23350 vs. NCBI nr
Match: KAG6607269.1 (Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542.0 bits (1395), Expect = 3.7e-150
Identity = 285/333 (85.59%), Postives = 294/333 (88.29%), Query Frame = 0


Query: 1   MLLVLVSPSSFPSFSSSTFNKSP--FSTFSSFPILCSFNSFCLFPSHSTHKLIKRLNPIR 60
           M L LVSPS   SFSSSTFN S    STFSS PI  SF  F L  S+STHKL+KRL  IR
Sbjct: 1   MFLALVSPSPSDSFSSSTFNPSQPHLSTFSSSPISRSFKPFWLPTSNSTHKLVKRLKSIR 60

Query: 61  NQTESDFNSKYGRTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGE 120
           NQ ESDF +K G TP D RWSL G TALVTGGTRGIGRAIVEELVGFGARVHTCSRNE E
Sbjct: 61  NQAESDFKTKNGSTPGDHRWSLHGTTALVTGGTRGIGRAIVEELVGFGARVHTCSRNENE 120

Query: 121 LRQCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTE 180
           L+QCLR WKD EFEVSGSVCDVSVRAQREELM+   +TFDGKLNILVNNVGRNIRKPTTE
Sbjct: 121 LKQCLRDWKDLEFEVSGSVCDVSVRAQREELMKTVADTFDGKLNILVNNVGRNIRKPTTE 180

Query: 181 FTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGA 240
           FTDEEFSFLMKTNVESVFHLSQLA+P LK+SG GSIVFMSS+SSFVSLKSMSVQGATKGA
Sbjct: 181 FTDEEFSFLMKTNVESVFHLSQLAHPFLKASGAGSIVFMSSVSSFVSLKSMSVQGATKGA 240

Query: 241 INQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEV 300
           INQLTKYLACEWA DNIRSNAVAPWYIKTSMVEQVLSNKAY EEVYSRTPLRRLGEPSEV
Sbjct: 241 INQLTKYLACEWANDNIRSNAVAPWYIKTSMVEQVLSNKAYVEEVYSRTPLRRLGEPSEV 300

Query: 301 SSLVAFFCLPASSYITGQIIGVDGGMSVNGFDP 332
           SSLVAF CLPASSYITGQIIGVDGGMSVNGFDP
Sbjct: 301 SSLVAFLCLPASSYITGQIIGVDGGMSVNGFDP 333

[Close]

BLAST of CSPI01G23350 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 384.8 bits (987), Expect = 7.0e-107
Identity = 200/286 (69.93%), Postives = 220/286 (76.92%), Query Frame = 0


Query: 52  KRLNPIRNQTESDFNSKYG-----RTPVDDRWSLRGKTALVTGGTRGIGRAIVEELVGFG 111
           KR+NP    + S     Y           +RWSL G +ALVTGGTRGIGRAIVEEL G G
Sbjct: 35  KRINPKHKSSSSSVFVPYASQSSIAITSKERWSLNGMSALVTGGTRGIGRAIVEELAGLG 94

Query: 112 ARVHTCSRNEGELRQCLRHWKDSEFEVSGSVCDVSVRAQREELMENAGNTFDGKLNILVN 171
           A VHTC+RNE EL  CL  W  S F V+GSVCDVS R+QRE LME   + F+GKL+ILVN
Sbjct: 95  AEVHTCARNEYELENCLSDWNRSGFRVAGSVCDVSDRSQREALMETVSSVFEGKLHILVN 154

Query: 172 NVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSL 231
           NVG NIRKP  EFT  EFS LM TN ESVFHL QLAYPLL+ S  GS+VF+SS+S FVSL
Sbjct: 155 NVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSL 214

Query: 232 KSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSR 291
           K+MSVQ +TKGAINQLT+ LACEWAKDNIR NAVAPWYIKTSMVEQVLSNK Y EEVYS 
Sbjct: 215 KNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTSMVEQVLSNKEYLEEVYSV 274

Query: 292 TPLRRLGEPSEVSSLVAFFCLPASSYITGQIIGVDGGMSVNGFDPK 333
           TPL RLGEP EVSS VAF CLPASSYITGQI+ VDGGMS+NGF P+
Sbjct: 275 TPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSINGFFPR 320

[Close]

BLAST of CSPI01G23350 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 3.7e-100
Identity = 176/255 (69.02%), Postives = 208/255 (81.57%), Query Frame = 0


Query: 75  DDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEVS 134
           D RWSL GKTALVTGGTRGIGRA+VEEL  FGA+VHTCSRN+ EL  CL  WK +   VS
Sbjct: 4   DKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVS 63

Query: 135 GSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVES 194
           GSVCD SVR QRE+L++ A + F GKLNIL+NNVG N+RKPT E++ EE++ +M TN+ES
Sbjct: 64  GSVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLES 123

Query: 195 VFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKDN 254
            FHLSQ+A+PLLK+SG GSIVF+SS++  V L S S+ GATKGA+NQLT+ LACEWA DN
Sbjct: 124 AFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDN 183

Query: 255 IRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYIT 314
           IR+N VAPWYIKTS+VE +L  K + E V SRTPL R+GEP EVSSLVAF CLPASSYIT
Sbjct: 184 IRTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYIT 243

Query: 315 GQIIGVDGGMSVNGF 330
           GQ+I VDGG +VNGF
Sbjct: 244 GQVISVDGGFTVNGF 258

[Close]

BLAST of CSPI01G23350 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.5e-88
Identity = 158/256 (61.72%), Postives = 202/256 (78.91%), Query Frame = 0


Query: 74  VDDRWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEV 133
           +D RWSL+G  ALVTGGT+GIG A+VEEL   GARVHTC+R+E +L++ LR W++  F+V
Sbjct: 1   MDKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQV 60

Query: 134 SGSVCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVE 193
           + S+CDVS+R QRE+LME   + F GKLNILVNNVG  + KPTTE+T EEFSFLM TN++
Sbjct: 61  TTSICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLD 120

Query: 194 SVFHLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKD 253
           S FH+SQLA+PLLK+SG GSIV MSSI+  V +   S+ GATKGA+NQL + LACEWA D
Sbjct: 121 SAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASD 180

Query: 254 NIRSNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYI 313
           NIR+NA+ PW I T ++  +LS +  ++E   RTP+ R+GE +EVS LVAF CLPA+SYI
Sbjct: 181 NIRTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYI 240

Query: 314 TGQIIGVDGGMSVNGF 330
           TGQ+I VDGG++VNGF
Sbjct: 241 TGQVICVDGGLTVNGF 256

[Close]

BLAST of CSPI01G23350 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.4e-86
Identity = 157/256 (61.33%), Postives = 198/256 (77.34%), Query Frame = 0


Query: 77  RWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEVSGS 136
           RWSL G TALVTGG++GIG A+VEEL   GAR+HTC+R+E +L++ LR W+   F+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 137 VCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVF 196
           VCDVS R +RE+LME     F+GKLNILVNNVG  I KPT + T E+FSF M TN+ES F
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLESAF 132

Query: 197 HLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIR 256
           HLSQLA+PLLK+SG GSIV +SS+S  V +   S+ G +KGA+NQL + LACEWA DNIR
Sbjct: 133 HLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDNIR 192

Query: 257 SNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYITGQ 316
           +N+V PW+I+T +V + LSN+ + +EV SR P+ R+GE +EVSSLVAF CLPA+SYITGQ
Sbjct: 193 TNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQ 252

Query: 317 IIGVDGGMSVNGFDPK 333
            I VDGG +VNGF  K
Sbjct: 253 TICVDGGFTVNGFSFK 268

[Close]

BLAST of CSPI01G23350 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 313.9 bits (803), Expect = 1.5e-85
Identity = 154/253 (60.87%), Postives = 197/253 (77.87%), Query Frame = 0


Query: 77  RWSLRGKTALVTGGTRGIGRAIVEELVGFGARVHTCSRNEGELRQCLRHWKDSEFEVSGS 136
           +WSL G TALVTGG++G+G+A+VEEL   GARVHTC+R+E +L++ LR W+    +V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 137 VCDVSVRAQREELMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVF 196
           VCDVS R QRE+LME   + F GKL+ILV NVG  + KPTTE T EEFSF++ TN+ES F
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVPNVGIGVLKPTTECTAEEFSFIIATNLESTF 132

Query: 197 HLSQLAYPLLKSSGEGSIVFMSSISSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIR 256
           H SQLA+PLLK+SG G+IV MSS++  V+L + S+ GATKGA+NQL + LACEWA DNIR
Sbjct: 133 HFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDNIR 192

Query: 257 SNAVAPWYIKTSMVEQVLSNKAYEEEVYSRTPLRRLGEPSEVSSLVAFFCLPASSYITGQ 316
           +N+V PW+I T   +  L +K  +E+V S TPLRR+GE +EVSSLVAF CLPA+SYITGQ
Sbjct: 193 ANSVCPWFITTPSTKDFLGDKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASYITGQ 252

Query: 317 IIGVDGGMSVNGF 330
            I VDGG ++NGF
Sbjct: 253 TICVDGGFTINGF 265

The following BLAST results are available for this feature:

BLAST of CSPI01G23350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZW12	9.9e-106	69.93	Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=...	[more]
Q9LHT0	5.3e-99	69.02	Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060...	[more]
Q9ZW13	2.1e-87	61.72	Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292...	[more]
Q9ZW19	1.9e-85	61.33	Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=...	[more]
Q9ZW20	2.1e-84	60.87	Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g293...	[more]

BLAST of CSPI01G23350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BGS1	3.2e-176	96.08	Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C5I5	3.2e-176	96.08	tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE...	[more]
A0A6J1KAL5	3.0e-150	85.29	tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1GAE7	2.0e-149	84.98	tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1CL23	3.2e-147	82.83	tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC...	[more]

BLAST of CSPI01G23350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031741141.1	5.6e-183	99.10	tropinone reductase homolog At5g06060 [Cucumis sativus]	[more]
XP_008457446.1	6.7e-176	96.08	PREDICTED: tropinone reductase homolog At5g06060 [Cucumis melo] >TYJ97378.1 trop...	[more]
XP_038893799.1	7.9e-161	88.66	tropinone reductase homolog At5g06060-like [Benincasa hispida]	[more]
KAE8653260.1	7.2e-154	99.29	hypothetical protein Csa_019809 [Cucumis sativus]	[more]
KAG6607269.1	3.7e-150	85.59	Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of CSPI01G23350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29260.1	7.0e-107	69.93	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G06060.1	3.7e-100	69.02	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29290.2	1.5e-88	61.72	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29360.1	1.4e-86	61.33	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29370.1	1.5e-85	60.87	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 232..251 score: 31.56 coord: 212..220 score: 42.61 coord: 159..170 score: 43.41
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 232..251 score: 36.93 coord: 253..270 score: 39.59 coord: 84..101 score: 51.89 coord: 159..170 score: 50.8 coord: 288..308 score: 30.89 coord: 206..222 score: 29.32
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 91..325 e-value: 1.6E-58 score: 198.1
None	No IPR available	GENE3D	3.40.50.720		coord: 73..328 e-value: 2.7E-79 score: 267.9
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 79..329 e-value: 5.6E-21 score: 72.7
None	No IPR available	PANTHER	PTHR42898:SF2	TROPINONE REDUCTASE-LIKE PROTEIN, CHLOROPLASTIC	coord: 59..332
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 59..332
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 81..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G23350.1	CSPI01G23350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity