CSPI01G22650 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G22650
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	Chr1: 18202349 .. 18203081 (-)
RNA-Seq Expression	CSPI01G22650
Synteny	CSPI01G22650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACGTCACTCCAAATTTCTCTGTTCTTGACACACCTGTAACGAGTTTTTTCAAATCCACCGCTGAATCAAAATATTAAATCAACTTACAACAGTGAAGCTAGATAGAAGCAACTATTTATTGTGGAAGACGCTAGCTCTACCCATTCTGAAAGGATACAAGTTAGAAAGACATTTAACAGGTGAGAAACCTTGCCCTGAAAAGTTTATCACTTCACTACCAGTGCATGTTCTAATACCTCGGTCATGGAAGGAGGAGATGACCATGAACAAAGGGCTCTTGAACAAATAGCTTCTTCGAGCACATCAACATATGCCATTATGAATCCCTTGTTTGAACAATGGATAACGATTGATTTGCTACTGCTTAGCTAGTTGTACAACTCAGTGACACCTGAAGTAGTTGTTCAACTAATAGGCTTCACAAATGCCAAAGATATGTGGGAAGCAACACATGATTTCTTTGGCGTTCGATCAAGAGCAGAGGAGGACTTCCTTCGACAAACCTTTCAAACAACAAGAAAAGGTAATTCTAACATGGAGGATTATCTAAGAATTATGAAAACTAATGCTGACAATCTTGGCCAAGCCGAAAGTCCTATTCCGAGACGTGCCCTTATTTCACAGGTTTTGTTGGGATTGGATGAAGTTTATAATCCTGTCATAGTAGTCATTCAAGGTAAGCCAGAGATATCATGGCTTGATATGCAGTCAACTTCTAATTTTTGA

mRNA sequence

ATGGCCAACGTCACTCCAAATTTCTCTGTTCTTGACACACCTACGCTAGCTCTACCCATTCTGAAAGGATACAAGTTAGAAAGACATTTAACAGGTGAGAAACCTTGCCCTGAAAAGTTTATCACTTCACTACCATTGTACAACTCAGTGACACCTGAAGTAGTTGTTCAACTAATAGGCTTCACAAATGCCAAAGATATGTGGGAAGCAACACATGATTTCTTTGGCGTTCGATCAAGAGCAGAGGAGGACTTCCTTCGACAAACCTTTCAAACAACAAGAAAAGGTAATTCTAACATGGAGGATTATCTAAGAATTATGAAAACTAATGCTGACAATCTTGGCCAAGCCGAAAGTCCTATTCCGAGACGTGCCCTTATTTCACAGGTTTTGTTGGGATTGGATGAAGTTTATAATCCTGTCATAGTAGTCATTCAAGGTAAGCCAGAGATATCATGGCTTGATATGCAGTCAACTTCTAATTTTTGA

Coding sequence (CDS)

ATGGCCAACGTCACTCCAAATTTCTCTGTTCTTGACACACCTACGCTAGCTCTACCCATTCTGAAAGGATACAAGTTAGAAAGACATTTAACAGGTGAGAAACCTTGCCCTGAAAAGTTTATCACTTCACTACCATTGTACAACTCAGTGACACCTGAAGTAGTTGTTCAACTAATAGGCTTCACAAATGCCAAAGATATGTGGGAAGCAACACATGATTTCTTTGGCGTTCGATCAAGAGCAGAGGAGGACTTCCTTCGACAAACCTTTCAAACAACAAGAAAAGGTAATTCTAACATGGAGGATTATCTAAGAATTATGAAAACTAATGCTGACAATCTTGGCCAAGCCGAAAGTCCTATTCCGAGACGTGCCCTTATTTCACAGGTTTTGTTGGGATTGGATGAAGTTTATAATCCTGTCATAGTAGTCATTCAAGGTAAGCCAGAGATATCATGGCTTGATATGCAGTCAACTTCTAATTTTTGA

Protein sequence

MANVTPNFSVLDTPTLALPILKGYKLERHLTGEKPCPEKFITSLPLYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLRIMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQSTSNF*

Homology

[Close]

BLAST of CSPI01G22650 vs. ExPASy TrEMBL
Match: A0A0A0LXB7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G496800 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.1e-56
Identity = 113/113 (100.00%), Postives = 113/113 (100.00%), Query Frame = 0


Query: 46  LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLR 105
           LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLR
Sbjct: 22  LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLR 81

Query: 106 IMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS 159
           IMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS
Sbjct: 82  IMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS 134

[Close]

BLAST of CSPI01G22650 vs. ExPASy TrEMBL
Match: A0A5A7SIT7 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G00360 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.4e-52
Identity = 112/187 (59.89%), Postives = 126/187 (67.38%), Query Frame = 0


Query: 15  TLALPILKGYKLERHLTGEKPCPEKFITSLP----------------------------- 74
           TLALPILKGYKLE HLTGE PCP  F+ S                               
Sbjct: 45  TLALPILKGYKLEGHLTGETPCPSHFVLSASSSNTTVTEEGADATIGASSSITPRIVNSL 104

Query: 75  --------------LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQ 134
                         LYNS+TP+V +QL+GFTN +D+W+AT DFFGV+SRAEEDFLRQ  Q
Sbjct: 105 FEQWVTTDLLLLGWLYNSMTPDVAIQLMGFTNVEDLWDATQDFFGVQSRAEEDFLRQMLQ 164

Query: 135 TTRKGNSNMEDYLRIMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEI 159
           TTRKGN+ ME+YL +MKTN DNLGQ  SP+PRRALISQVLLGLDEVYN VIVVIQGKP+I
Sbjct: 165 TTRKGNTKMEEYLLVMKTNVDNLGQVGSPVPRRALISQVLLGLDEVYNLVIVVIQGKPDI 224

[Close]

BLAST of CSPI01G22650 vs. ExPASy TrEMBL
Match: A0A5D3E3L7 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001590 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 5.7e-41
Identity = 84/113 (74.34%), Postives = 96/113 (84.96%), Query Frame = 0


Query: 46  LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLR 105
           +YNS+ P+V +QL+GF  AKD+WEA  + FG++SRAEE FLR TFQTTR+GN  MEDYLR
Sbjct: 83  IYNSMVPDVALQLMGFNTAKDLWEAIQNLFGIKSRAEEYFLRHTFQTTREGNYKMEDYLR 142

Query: 106 IMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS 159
           IMK NADNLGQA SP+P R LISQVLLGLDEVYNPV  VIQGKP+ISWLDMQS
Sbjct: 143 IMKINADNLGQAGSPVPHRYLISQVLLGLDEVYNPVTAVIQGKPDISWLDMQS 195

[Close]

BLAST of CSPI01G22650 vs. ExPASy TrEMBL
Match: A0A5D3C373 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G002430 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 4.8e-40
Identity = 82/105 (78.10%), Postives = 93/105 (88.57%), Query Frame = 0


Query: 54  VVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLRIMKTNADN 113
           + +QL+GFTNAKD+WEAT D FGV+SRAEEDFLRQ FQTTRK  ++ EDYLRIMKTN+D 
Sbjct: 45  IAIQLMGFTNAKDLWEATQDLFGVQSRAEEDFLRQMFQTTRKVRASYEDYLRIMKTNSDK 104

Query: 114 LGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS 159
           LGQA SP+P+RA ISQ LLGLDEVYNPVI VIQGKPEISW+DMQS
Sbjct: 105 LGQAGSPVPKRAFISQALLGLDEVYNPVIAVIQGKPEISWIDMQS 149

[Close]

BLAST of CSPI01G22650 vs. ExPASy TrEMBL
Match: A0A5D3BCH9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00140 PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 1.1e-36
Identity = 85/158 (53.80%), Postives = 98/158 (62.03%), Query Frame = 0


Query: 15  TLALPILKGYKLERHLTGEKPCPEKFITSLP----------------------------- 74
           TLALPILKGYKLE HLTGE PCP  F+ S                               
Sbjct: 45  TLALPILKGYKLEGHLTGETPCPSHFVLSASSSNTTVTEEGADATIGASSSITPRIVNSL 104

Query: 75  --------------LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQ 130
                         LYNS+TP+V +QL+GFTN +D+W+AT DFFGV+SRAEEDFLRQ  Q
Sbjct: 105 FEQWVTTDLLLLGWLYNSMTPDVAIQLMGFTNVEDLWDATQDFFGVQSRAEEDFLRQMLQ 164

[Close]

BLAST of CSPI01G22650 vs. NCBI nr
Match: KGN65684.1 (hypothetical protein Csa_019689 [Cucumis sativus])

HSP 1 Score: 228.4 bits (581), Expect = 4.4e-56
Identity = 113/113 (100.00%), Postives = 113/113 (100.00%), Query Frame = 0


Query: 46  LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLR 105
           LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLR
Sbjct: 22  LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLR 81

Query: 106 IMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS 159
           IMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS
Sbjct: 82  IMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS 134

[Close]

BLAST of CSPI01G22650 vs. NCBI nr
Match: KAA0026100.1 (uncharacterized protein E6C27_scaffold19G00360 [Cucumis melo var. makuwa])

HSP 1 Score: 215.7 bits (548), Expect = 3.0e-52
Identity = 112/187 (59.89%), Postives = 126/187 (67.38%), Query Frame = 0


Query: 15  TLALPILKGYKLERHLTGEKPCPEKFITSLP----------------------------- 74
           TLALPILKGYKLE HLTGE PCP  F+ S                               
Sbjct: 45  TLALPILKGYKLEGHLTGETPCPSHFVLSASSSNTTVTEEGADATIGASSSITPRIVNSL 104

Query: 75  --------------LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQ 134
                         LYNS+TP+V +QL+GFTN +D+W+AT DFFGV+SRAEEDFLRQ  Q
Sbjct: 105 FEQWVTTDLLLLGWLYNSMTPDVAIQLMGFTNVEDLWDATQDFFGVQSRAEEDFLRQMLQ 164

Query: 135 TTRKGNSNMEDYLRIMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEI 159
           TTRKGN+ ME+YL +MKTN DNLGQ  SP+PRRALISQVLLGLDEVYN VIVVIQGKP+I
Sbjct: 165 TTRKGNTKMEEYLLVMKTNVDNLGQVGSPVPRRALISQVLLGLDEVYNLVIVVIQGKPDI 224

[Close]

BLAST of CSPI01G22650 vs. NCBI nr
Match: KAA0057475.1 (uncharacterized protein E6C27_scaffold280G003560 [Cucumis melo var. makuwa] >TYK30171.1 uncharacterized protein E5676_scaffold216G001590 [Cucumis melo var. makuwa])

HSP 1 Score: 177.2 bits (448), Expect = 1.2e-40
Identity = 84/113 (74.34%), Postives = 96/113 (84.96%), Query Frame = 0


Query: 46  LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLR 105
           +YNS+ P+V +QL+GF  AKD+WEA  + FG++SRAEE FLR TFQTTR+GN  MEDYLR
Sbjct: 83  IYNSMVPDVALQLMGFNTAKDLWEAIQNLFGIKSRAEEYFLRHTFQTTREGNYKMEDYLR 142

Query: 106 IMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS 159
           IMK NADNLGQA SP+P R LISQVLLGLDEVYNPV  VIQGKP+ISWLDMQS
Sbjct: 143 IMKINADNLGQAGSPVPHRYLISQVLLGLDEVYNPVTAVIQGKPDISWLDMQS 195

[Close]

BLAST of CSPI01G22650 vs. NCBI nr
Match: TYK05754.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 174.1 bits (440), Expect = 9.9e-40
Identity = 82/105 (78.10%), Postives = 93/105 (88.57%), Query Frame = 0


Query: 54  VVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYLRIMKTNADN 113
           + +QL+GFTNAKD+WEAT D FGV+SRAEEDFLRQ FQTTRK  ++ EDYLRIMKTN+D 
Sbjct: 45  IAIQLMGFTNAKDLWEATQDLFGVQSRAEEDFLRQMFQTTRKVRASYEDYLRIMKTNSDK 104

Query: 114 LGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQS 159
           LGQA SP+P+RA ISQ LLGLDEVYNPVI VIQGKPEISW+DMQS
Sbjct: 105 LGQAGSPVPKRAFISQALLGLDEVYNPVIAVIQGKPEISWIDMQS 149

[Close]

BLAST of CSPI01G22650 vs. NCBI nr
Match: TYJ96311.1 (uncharacterized protein E5676_scaffold1970G00140 [Cucumis melo var. makuwa])

HSP 1 Score: 162.9 bits (411), Expect = 2.3e-36
Identity = 85/158 (53.80%), Postives = 98/158 (62.03%), Query Frame = 0


Query: 15  TLALPILKGYKLERHLTGEKPCPEKFITSLP----------------------------- 74
           TLALPILKGYKLE HLTGE PCP  F+ S                               
Sbjct: 45  TLALPILKGYKLEGHLTGETPCPSHFVLSASSSNTTVTEEGADATIGASSSITPRIVNSL 104

Query: 75  --------------LYNSVTPEVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQ 130
                         LYNS+TP+V +QL+GFTN +D+W+AT DFFGV+SRAEEDFLRQ  Q
Sbjct: 105 FEQWVTTDLLLLGWLYNSMTPDVAIQLMGFTNVEDLWDATQDFFGVQSRAEEDFLRQMLQ 164

[Close]

BLAST of CSPI01G22650 vs. TAIR 10
Match: AT1G34070.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G48050.1); Has 648 Blast hits to 647 proteins in 29 species: Archae - 0; Bacteria - 0; Metazoa - 16; Fungi - 25; Plants - 607; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 46.6 bits (109), Expect = 2.2e-05
Identity = 33/120 (27.50%), Postives = 56/120 (46.67%), Query Frame = 0


Query: 41  ITSLPLYNSVTP-EVVVQLIGFTNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSN 100
           I  L LY ++TP +     +  + ++D+W    + F     A    L    +T   G+  
Sbjct: 71  IVKLSLYGTLTPKQFQGSFVTSSTSRDIWLRIKNQFRNNKDARALRLDSELRTKDIGDMR 130

Query: 101 MEDYLRIMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGKPEISWLDMQST 160
           + DY R MK  AD+L   + P+  R L+  VL GL+  ++ +I VI+ +      D  +T
Sbjct: 131 VADYYRKMKKLADSLRNVDVPVTDRNLVMYVLNGLNPKFDNIINVIKHRQPFPSFDDAAT 190

[Close]

BLAST of CSPI01G22650 vs. TAIR 10
Match: AT5G48050.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G34070.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 44.7 bits (104), Expect = 8.4e-05
Identity = 27/104 (25.96%), Postives = 53/104 (50.96%), Query Frame = 0


Query: 46  LYNSVTPEVVVQLIGF-TNAKDMWEATHDFFGVRSRAEEDFLRQTFQTTRKGNSNMEDYL 105
           +Y ++T  ++  +I     A+D+W +  + F     A         +TT   + ++ +Y 
Sbjct: 78  IYGTITDSLLDTIIKVGCTARDLWLSLENLFRDNKEARALQFENELRTTTIDDLSVHEYC 137

Query: 106 RIMKTNADNLGQAESPIPRRALISQVLLGLDEVYNPVIVVIQGK 149
           + +K+ +D L   +SPI  R L+  +L GL E Y+ ++ VI+ K
Sbjct: 138 QKLKSLSDLLTNVDSPISDRVLVMHLLNGLTEKYDYILNVIKHK 181

The following BLAST results are available for this feature:

BLAST of CSPI01G22650 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI01G22650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXB7	2.1e-56	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G496800 PE=4 SV=1	[more]
A0A5A7SIT7	1.4e-52	59.89	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3E3L7	5.7e-41	74.34	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3C373	4.8e-40	78.10	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var....	[more]
A0A5D3BCH9	1.1e-36	53.80	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of CSPI01G22650 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN65684.1	4.4e-56	100.00	hypothetical protein Csa_019689 [Cucumis sativus]	[more]
KAA0026100.1	3.0e-52	59.89	uncharacterized protein E6C27_scaffold19G00360 [Cucumis melo var. makuwa]	[more]
KAA0057475.1	1.2e-40	74.34	uncharacterized protein E6C27_scaffold280G003560 [Cucumis melo var. makuwa] >TYK...	[more]
TYK05754.1	9.9e-40	78.10	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]
TYJ96311.1	2.3e-36	53.80	uncharacterized protein E5676_scaffold1970G00140 [Cucumis melo var. makuwa]	[more]

BLAST of CSPI01G22650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G34070.1	2.2e-05	27.50	CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BE...	[more]
AT5G48050.1	8.4e-05	25.96	CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BE...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF14223	Retrotran_gag_2	coord: 46..147 e-value: 3.2E-9 score: 36.7
None	No IPR available	PANTHER	PTHR34222:SF48	SUBFAMILY NOT NAMED	coord: 17..146
None	No IPR available	PANTHER	PTHR34222	FAMILY NOT NAMED	coord: 17..146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G22650.1	CSPI01G22650.1	mRNA