CSPI01G22000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G22000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	phosphatidylinositol-glycan biosynthesis class X protein isoform X2
Location	Chr1: 17550030 .. 17551427 (+)
RNA-Seq Expression	CSPI01G22000
Synteny	CSPI01G22000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTGGAAGACATATTATGGAATCCTATTATGAAAAACACGAAAGTTTTATTGATTCACTTTTTGATGATTTCCTGACACGAGATCTTCCCGTCAACATATGTGAAGTTTTACCAAGCGATCTAAATACATTGCTAAGTCTCTCAATTTGGTCAAGAATCAAGGGTGACGGTTCTCACCGTCAACTTTCATCGACCATCAAGTTCAACACTGAAAAATCCCTGTTTCAGGTTCCCACACACCACTGTAAGTTTATAATCATTGAACGACTACCATCGGGAGTTTTTGTTGACCCATTTGAATTGGAACATCTTCTTCATCGAGCTGGTAAATCAGATTTAAATTTCTCTTGTTTCTTTCCATGATTTTTTTCCGTTTTCTAGCCTAAGAAAATGGCATTGTCTTTATGGTAAGTTCAACCCTTTTCTTGTACTGTTTTCTTCTTTTGTCAGTATTTAGTGATGTAGCTGTTTTTGGAGATACAAATTTGGAATTGCCTTCAGTCCTCTCCAACATTTCTGTTGTCGAGGTTCACAAGGACATTGGTCTCAATATCTTGTCACACAACAAGAATCTGATTGATTTCACCGTTGATCTGCCTTTACATTCACGATATCCAGTAAGCCTGAAATATCTTTTAGAATACTGACCTAGGTGATTTTGGTTTTTATCAAGTTTATTATGAATAATCTGGGATCATGGATGTAATGGAATTTGAAAACTTCTTACTGTAAACTTAGCTTATATAACATTGGATAAAGTATTCATGTCTCATGCTCCAACACTCATGAAGCGCGTTAGATTTTTCCAGAATTGAGTGCGCATTCTTTGAATTCATATCAAATTCTTGATAAGTACAATAAACAGATGTCAATGCTTTGAATATTTTCTGAGCAAATGTTTGGAGTGCAAAGACATAATGCATAGCGGTTATTGACGTTGAATTTTTTTTTCATAATGTGTTGATATTTTAAGATTGTTTCACCACAAACCGTTCATTTTATTCACTTAAATTGGTTAGTAATTAAATTTTCCTGCTGTTTTGCAGCGATTGGATGAAAGTGGTTACGTGGAAGTTAGACTGAAGGCTCCAGACTTGTTTTTGCAGTGTAATATACAGGAAAAACCACACAATAGGACCTGTTTTTTTAGGTTGGAAACTGATGATGAAGAGGCAGATCTTACATGGTCGATACCAGCAGGTAAACGATCTGATGCTGGAGTTGTTAGCGCTATTACTTTTGTTTCTGCTTTGTTATCAGTACTATGCATTGTATTTTCTACACAAGTTAGACAACCCAAAGTTTTGAAGCAATGTTAACTGATTCAACAATTCTTCTTCTTTTCAAGAGTACGTTTAGTCTTGAGCCTGCTCAAGAGGTGTAACATTTTCGTTGGG

mRNA sequence

GGTTGGAAGACATATTATGGAATCCTATTATGAAAAACACGAAAGTTTTATTGATTCACTTTTTGATGATTTCCTGACACGAGATCTTCCCGTCAACATATGTGAAGTTTTACCAAGCGATCTAAATACATTGCTAAGTCTCTCAATTTGGTCAAGAATCAAGGGTGACGGTTCTCACCGTCAACTTTCATCGACCATCAAGTTCAACACTGAAAAATCCCTGTTTCAGGTTCCCACACACCACTGTAAGTTTATAATCATTGAACGACTACCATCGGGAGTTTTTGTTGACCCATTTGAATTGGAACATCTTCTTCATCGAGCTGTATTTAGTGATGTAGCTGTTTTTGGAGATACAAATTTGGAATTGCCTTCAGTCCTCTCCAACATTTCTGTTGTCGAGGTTCACAAGGACATTGGTCTCAATATCTTGTCACACAACAAGAATCTGATTGATTTCACCGTTGATCTGCCTTTACATTCACGATATCCACGATTGGATGAAAGTGGTTACGTGGAAGTTAGACTGAAGGCTCCAGACTTGTTTTTGCAGTGTAATATACAGGAAAAACCACACAATAGGACCTGTTTTTTTAGGTTGGAAACTGATGATGAAGAGGCAGATCTTACATGGTCGATACCAGCAGGTAAACGATCTGATGCTGGAGTTGTTAGCGCTATTACTTTTGTTTCTGCTTTGTTATCAGTACTATGCATTGTATTTTCTACACAAGTTAGACAACCCAAAGTTTTGAAGCAATGTTAACTGATTCAACAATTCTTCTTCTTTTCAAGAGTACGTTTAGTCTTGAGCCTGCTCAAGAGGTGTAACATTTTCGTTGGG

Coding sequence (CDS)

ATGGAATCCTATTATGAAAAACACGAAAGTTTTATTGATTCACTTTTTGATGATTTCCTGACACGAGATCTTCCCGTCAACATATGTGAAGTTTTACCAAGCGATCTAAATACATTGCTAAGTCTCTCAATTTGGTCAAGAATCAAGGGTGACGGTTCTCACCGTCAACTTTCATCGACCATCAAGTTCAACACTGAAAAATCCCTGTTTCAGGTTCCCACACACCACTGTAAGTTTATAATCATTGAACGACTACCATCGGGAGTTTTTGTTGACCCATTTGAATTGGAACATCTTCTTCATCGAGCTGTATTTAGTGATGTAGCTGTTTTTGGAGATACAAATTTGGAATTGCCTTCAGTCCTCTCCAACATTTCTGTTGTCGAGGTTCACAAGGACATTGGTCTCAATATCTTGTCACACAACAAGAATCTGATTGATTTCACCGTTGATCTGCCTTTACATTCACGATATCCACGATTGGATGAAAGTGGTTACGTGGAAGTTAGACTGAAGGCTCCAGACTTGTTTTTGCAGTGTAATATACAGGAAAAACCACACAATAGGACCTGTTTTTTTAGGTTGGAAACTGATGATGAAGAGGCAGATCTTACATGGTCGATACCAGCAGGTAAACGATCTGATGCTGGAGTTGTTAGCGCTATTACTTTTGTTTCTGCTTTGTTATCAGTACTATGCATTGTATTTTCTACACAAGTTAGACAACCCAAAGTTTTGAAGCAATGTTAA

Protein sequence

MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSSTIKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPSVLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQCNIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQVRQPKVLKQC*

Homology

[Close]

BLAST of CSPI01G22000 vs. ExPASy Swiss-Prot
Match: Q8TBF5 (Phosphatidylinositol-glycan biosynthesis class X protein OS=Homo sapiens OX=9606 GN=PIGX PE=2 SV=3)

HSP 1 Score: 64.7 bits (156), Expect = 1.7e-09
Identity = 45/138 (32.61%), Postives = 67/138 (48.55%), Query Frame = 0


Query: 51  DGSHRQLSSTIKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAV 110
           DG HR L   +KF           H C+ +I + +P+G++VDP+EL  L  R +   V V
Sbjct: 55  DGFHRDLLIKVKFGESIE----DLHTCRLLIKQDIPAGLYVDPYELASLRERNITEAVMV 114

Query: 111 FGDTNLELPSVLSNISVVEVHKDIGLNILSHNKNLID-FTVDLPLHSRYPR-LDESGYVE 170
             + ++E P+ LS  S V ++          +   ID F   LP+H RY R   E G   
Sbjct: 115 SENFDIEAPNYLSKESEVLIY-------ARRDSQCIDCFQAFLPVHCRYHRPHSEDGEAS 174

Query: 171 VRLKAPDLFLQCNIQEKP 187
           + +  PDL + C+ QE P
Sbjct: 175 IVVNNPDLLMFCD-QEFP 180

[Close]

BLAST of CSPI01G22000 vs. ExPASy Swiss-Prot
Match: Q60GF7 (Phosphatidylinositol-glycan biosynthesis class X protein OS=Rattus norvegicus OX=10116 GN=Pigx PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 62/204 (30.39%), Postives = 93/204 (45.59%), Query Frame = 0


Query: 51  DGSHRQLSSTIKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAV 110
           DG HR L   +KF   +S+  + T  C+ +I   +P+G+FVDP+EL  L  R +   V V
Sbjct: 49  DGFHRDLLIKVKFG--ESIEDLQT--CRLLIKHYIPTGLFVDPYELASLRERNITEAVMV 108

Query: 111 FGDTNLELPSVLSNISVVEVHKDIGLNILSHNKNLID-FTVDLPLHSRYPR-LDESGYVE 170
               NLE P+ LS  S V ++          +   ID F   LP+H RY R   + G   
Sbjct: 109 SESFNLEAPNYLSTESAVLIY-------ARQDAQCIDCFQAFLPVHYRYHRPHKKDGDTL 168

Query: 171 VRLKAPDLFLQCNIQEKPHNRTCFFRLET------DDEE-------------ADLTWSIP 230
           + +  PDL + C+ QE P  + C+ + E         EE              +LT  +P
Sbjct: 169 IVVNNPDLLMHCD-QEFPILK-CWAQSEVAAPCSLKSEEICQWKNMQYKSILKNLTVQVP 228

Query: 231 AGKRSDAGVVSAITFVSALLSVLC 234
            G      +V ++T    L++VLC
Sbjct: 229 VGLTIHTSLVCSVTL---LITVLC 236

[Close]

BLAST of CSPI01G22000 vs. ExPASy Swiss-Prot
Match: Q99LV7 (Phosphatidylinositol-glycan biosynthesis class X protein OS=Mus musculus OX=10090 GN=Pigx PE=2 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 8.4e-09
Identity = 60/204 (29.41%), Postives = 93/204 (45.59%), Query Frame = 0


Query: 51  DGSHRQLSSTIKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAV 110
           DG HR L   +KF   +S+  + T  C+ +I   +P G+FVDP+EL  L  R +   V +
Sbjct: 51  DGFHRDLLIKVKFG--ESIEDLQT--CRLLIKHYIPPGLFVDPYELASLRERNLTEAVML 110

Query: 111 FGDTNLELPSVLSNISVVEVHKDIGLNILSHNKNLID-FTVDLPLHSRYPR-LDESGYVE 170
               N+E P+ LSN S V ++          +   ID F   LP+H RY R   + G   
Sbjct: 111 SESFNIEAPNYLSNESAVLIY-------ARQDAQCIDCFQAFLPVHYRYHRPHKKDGDTL 170

Query: 171 VRLKAPDLFLQCNIQEKPHNRTCFFRLET------DDEE-------------ADLTWSIP 230
           + +  PDL + C+ QE P  + C+ + E         EE              +LT  +P
Sbjct: 171 IVVNNPDLLMYCD-QEFPILK-CWAQSEVAAPCALKSEEICQWKSMQYKSILKNLTVQVP 230

Query: 231 AGKRSDAGVVSAITFVSALLSVLC 234
            G      +V ++T    L+++LC
Sbjct: 231 VGLTIHTSLVCSVTL---LITILC 238

[Close]

BLAST of CSPI01G22000 vs. ExPASy TrEMBL
Match: A0A0A0LV34 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G470340 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.2e-124
Identity = 225/230 (97.83%), Postives = 227/230 (98.70%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST
Sbjct: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKF  EKSLF VPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDT+LELPS
Sbjct: 61  IKFKIEKSLFHVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTHLELPS 120

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLS 231
           +IQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLS
Sbjct: 181 SIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLS 230

[Close]

BLAST of CSPI01G22000 vs. ExPASy TrEMBL
Match: A0A1S3B562 (phosphatidylinositol-glycan biosynthesis class X protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486129 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.2e-124
Identity = 223/244 (91.39%), Postives = 235/244 (96.31%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLT+DLPVN+C++LP DLNT+LSLSI SRIKGDGSHRQLSST
Sbjct: 43  MESYYEKHESFIDSLFDDFLTQDLPVNLCKILPCDLNTVLSLSIQSRIKGDGSHRQLSST 102

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKFN  KSL QVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 103 IKFNIGKSLSQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 162

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSN SVVEVHKDIGLNILSHNKNL+DFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 163 VLSNFSVVEVHKDIGLNILSHNKNLLDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 222

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 240
           +IQEKP NR+C F+LETDDEEADLTWSIPAGKRSDAG+VS +TFVSAL+SVLCIVFSTQV
Sbjct: 223 SIQEKPLNRSCLFKLETDDEEADLTWSIPAGKRSDAGIVSVVTFVSALVSVLCIVFSTQV 282

Query: 241 RQPK 245
           RQPK
Sbjct: 283 RQPK 286

[Close]

BLAST of CSPI01G22000 vs. ExPASy TrEMBL
Match: A0A1S3B564 (phosphatidylinositol-glycan biosynthesis class X protein isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486129 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.2e-124
Identity = 223/244 (91.39%), Postives = 235/244 (96.31%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLT+DLPVN+C++LP DLNT+LSLSI SRIKGDGSHRQLSST
Sbjct: 1   MESYYEKHESFIDSLFDDFLTQDLPVNLCKILPCDLNTVLSLSIQSRIKGDGSHRQLSST 60

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKFN  KSL QVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 61  IKFNIGKSLSQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSN SVVEVHKDIGLNILSHNKNL+DFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 121 VLSNFSVVEVHKDIGLNILSHNKNLLDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 240
           +IQEKP NR+C F+LETDDEEADLTWSIPAGKRSDAG+VS +TFVSAL+SVLCIVFSTQV
Sbjct: 181 SIQEKPLNRSCLFKLETDDEEADLTWSIPAGKRSDAGIVSVVTFVSALVSVLCIVFSTQV 240

Query: 241 RQPK 245
           RQPK
Sbjct: 241 RQPK 244

[Close]

BLAST of CSPI01G22000 vs. ExPASy TrEMBL
Match: A0A5D3BTA5 (Phosphatidylinositol-glycan biosynthesis class X protein isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1738G00130 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 4.8e-116
Identity = 208/229 (90.83%), Postives = 220/229 (96.07%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLT+DLPVN+C++LP DLNT+LSLSI SRIKGDGSHRQLSST
Sbjct: 1   MESYYEKHESFIDSLFDDFLTQDLPVNVCKILPCDLNTVLSLSIQSRIKGDGSHRQLSST 60

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKFN  KSL QVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 61  IKFNIGKSLSQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSN SVVEVHKDIGLNILSHNKNL+DFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 121 VLSNFSVVEVHKDIGLNILSHNKNLLDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALL 230
           +IQEKP NR+C F+LETDDEEADLTWSIPAGKRSDAG+VS +TFVSAL+
Sbjct: 181 SIQEKPLNRSCLFKLETDDEEADLTWSIPAGKRSDAGIVSVVTFVSALI 229

[Close]

BLAST of CSPI01G22000 vs. ExPASy TrEMBL
Match: A0A5A7TCL5 (Phosphatidylinositol-glycan biosynthesis class X protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G001530 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 6.3e-116
Identity = 208/229 (90.83%), Postives = 220/229 (96.07%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLT+DLPVN+C++LP DLNT+LSLSI SRIKGDGSHRQLSST
Sbjct: 62  MESYYEKHESFIDSLFDDFLTQDLPVNLCKILPCDLNTVLSLSIQSRIKGDGSHRQLSST 121

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKFN  KSL QVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 122 IKFNIGKSLSQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 181

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSN SVVEVHKDIGLNILSHNKNL+DFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 182 VLSNFSVVEVHKDIGLNILSHNKNLLDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 241

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALL 230
           +IQEKP NR+C F+LETDDEEADLTWSIPAGKRSDAG+VS +TFVSAL+
Sbjct: 242 SIQEKPLNRSCLFKLETDDEEADLTWSIPAGKRSDAGIVSVVTFVSALI 290

[Close]

BLAST of CSPI01G22000 vs. NCBI nr
Match: XP_011657626.2 (phosphatidylinositol-glycan biosynthesis class X protein isoform X1 [Cucumis sativus])

HSP 1 Score: 493.8 bits (1270), Expect = 8.7e-136
Identity = 245/249 (98.39%), Postives = 246/249 (98.80%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST
Sbjct: 51  MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 110

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKF  EKSLF VPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 111 IKFKIEKSLFHVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 170

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 171 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 230

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 240
           +IQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV
Sbjct: 231 SIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 290

Query: 241 RQPKVLKQC 250
           RQPKVLKQC
Sbjct: 291 RQPKVLKQC 299

[Close]

BLAST of CSPI01G22000 vs. NCBI nr
Match: XP_031736304.1 (phosphatidylinositol-glycan biosynthesis class X protein isoform X2 [Cucumis sativus])

HSP 1 Score: 493.8 bits (1270), Expect = 8.7e-136
Identity = 245/249 (98.39%), Postives = 246/249 (98.80%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST
Sbjct: 44  MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 103

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKF  EKSLF VPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 104 IKFKIEKSLFHVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 163

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 164 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 223

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 240
           +IQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV
Sbjct: 224 SIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 283

Query: 241 RQPKVLKQC 250
           RQPKVLKQC
Sbjct: 284 RQPKVLKQC 292

[Close]

BLAST of CSPI01G22000 vs. NCBI nr
Match: XP_008442206.1 (PREDICTED: phosphatidylinositol-glycan biosynthesis class X protein isoform X1 [Cucumis melo])

HSP 1 Score: 454.9 bits (1169), Expect = 4.5e-124
Identity = 223/244 (91.39%), Postives = 235/244 (96.31%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLT+DLPVN+C++LP DLNT+LSLSI SRIKGDGSHRQLSST
Sbjct: 43  MESYYEKHESFIDSLFDDFLTQDLPVNLCKILPCDLNTVLSLSIQSRIKGDGSHRQLSST 102

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKFN  KSL QVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 103 IKFNIGKSLSQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 162

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSN SVVEVHKDIGLNILSHNKNL+DFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 163 VLSNFSVVEVHKDIGLNILSHNKNLLDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 222

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 240
           +IQEKP NR+C F+LETDDEEADLTWSIPAGKRSDAG+VS +TFVSAL+SVLCIVFSTQV
Sbjct: 223 SIQEKPLNRSCLFKLETDDEEADLTWSIPAGKRSDAGIVSVVTFVSALVSVLCIVFSTQV 282

Query: 241 RQPK 245
           RQPK
Sbjct: 283 RQPK 286

[Close]

BLAST of CSPI01G22000 vs. NCBI nr
Match: XP_008442207.1 (PREDICTED: phosphatidylinositol-glycan biosynthesis class X protein isoform X2 [Cucumis melo])

HSP 1 Score: 454.9 bits (1169), Expect = 4.5e-124
Identity = 223/244 (91.39%), Postives = 235/244 (96.31%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESYYEKHESFIDSLFDDFLT+DLPVN+C++LP DLNT+LSLSI SRIKGDGSHRQLSST
Sbjct: 1   MESYYEKHESFIDSLFDDFLTQDLPVNLCKILPCDLNTVLSLSIQSRIKGDGSHRQLSST 60

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKFN  KSL QVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 61  IKFNIGKSLSQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSN SVVEVHKDIGLNILSHNKNL+DFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC
Sbjct: 121 VLSNFSVVEVHKDIGLNILSHNKNLLDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 240
           +IQEKP NR+C F+LETDDEEADLTWSIPAGKRSDAG+VS +TFVSAL+SVLCIVFSTQV
Sbjct: 181 SIQEKPLNRSCLFKLETDDEEADLTWSIPAGKRSDAGIVSVVTFVSALVSVLCIVFSTQV 240

Query: 241 RQPK 245
           RQPK
Sbjct: 241 RQPK 244

[Close]

BLAST of CSPI01G22000 vs. NCBI nr
Match: XP_038881271.1 (phosphatidylinositol-glycan biosynthesis class X protein isoform X2 [Benincasa hispida] >XP_038881272.1 phosphatidylinositol-glycan biosynthesis class X protein isoform X2 [Benincasa hispida] >XP_038881274.1 phosphatidylinositol-glycan biosynthesis class X protein isoform X2 [Benincasa hispida])

HSP 1 Score: 434.9 bits (1117), Expect = 4.8e-118
Identity = 214/249 (85.94%), Postives = 231/249 (92.77%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           M SYYEK+ESFID+ FD FL++DLPVNICEVLP DLNT LSLSI SRI G+GSHRQLSST
Sbjct: 1   MGSYYEKYESFIDAHFDGFLSQDLPVNICEVLPCDLNTALSLSIRSRINGEGSHRQLSST 60

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IKFN +KS+ QVP  HCKFIIIERLPSGVF DPFELEHLLHRAVFSDVAVFGDTNLELPS
Sbjct: 61  IKFNIKKSMSQVPIQHCKFIIIERLPSGVFADPFELEHLLHRAVFSDVAVFGDTNLELPS 120

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFLQC 180
           VLSNISVVEVHKDIG NILSHNKNL+DFTVDLPLHSRYPRLDESGYV+VRLKAPDLFLQC
Sbjct: 121 VLSNISVVEVHKDIGPNILSHNKNLLDFTVDLPLHSRYPRLDESGYVQVRLKAPDLFLQC 180

Query: 181 NIQEKPHNRTCFFRLETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCIVFSTQV 240
           +IQEKPHNR+C F+LE+DD EADLTWSIPAGKRSDAG+V A+TFVSALLSVLCIVF TQ+
Sbjct: 181 SIQEKPHNRSCLFKLESDDAEADLTWSIPAGKRSDAGIVRAVTFVSALLSVLCIVFFTQI 240

Query: 241 RQPKVLKQC 250
           RQ KV+KQC
Sbjct: 241 RQLKVMKQC 249

[Close]

BLAST of CSPI01G22000 vs. TAIR 10
Match: AT5G46850.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 151.4 bits (381), Expect = 9.8e-37
Identity = 104/256 (40.62%), Postives = 135/256 (52.73%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESY+  + S  DS F +FL      +      S++    S      I G+GSHR L ++
Sbjct: 48  MESYFTTYTSLDDSEFRNFLLLQQCSDKVPTQDSNIVPATSFIQHRNITGEGSHRNLITS 107

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IK     S  Q      + +I+ERLP GVF DPFEL+ L  R  FSDV+VFGDTNLELPS
Sbjct: 108 IKLLLHHSESQFR----ELVIVERLPLGVFADPFELQSLQQRRAFSDVSVFGDTNLELPS 167

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFL-Q 180
             SN SVVE+H +I     S N    + +  LPLH+RY  LD+SGY  V    PDLFL  
Sbjct: 168 FRSNRSVVEIHVEIS----SRNYENGEISFKLPLHARYQPLDDSGYSRVEFGEPDLFLCS 227

Query: 181 CNIQ-EKPHNRTCFFRL--ETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCI-- 240
            ++Q ++   R C       +  E   + W IPAG R     VSA+TF +A+ S   I  
Sbjct: 228 SHVQNQRRERRRCLVLSIGRSKTETRSVFWDIPAGIRGHTEYVSALTFAAAVFSAFSILV 287

Query: 241 --VFSTQVRQPKVLKQ 249
             V S++    K LKQ
Sbjct: 288 ASVLSSKGESCKNLKQ 295

[Close]

BLAST of CSPI01G22000 vs. TAIR 10
Match: AT5G46850.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic process; LOCATED IN: endoplasmic reticulum membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: PIG-X/PBN1 (InterPro:IPR013233). )

HSP 1 Score: 151.4 bits (381), Expect = 9.8e-37
Identity = 104/256 (40.62%), Postives = 135/256 (52.73%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESY+  + S  DS F +FL      +      S++    S      I G+GSHR L ++
Sbjct: 77  MESYFTTYTSLDDSEFRNFLLLQQCSDKVPTQDSNIVPATSFIQHRNITGEGSHRNLITS 136

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IK     S  Q      + +I+ERLP GVF DPFEL+ L  R  FSDV+VFGDTNLELPS
Sbjct: 137 IKLLLHHSESQFR----ELVIVERLPLGVFADPFELQSLQQRRAFSDVSVFGDTNLELPS 196

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFL-Q 180
             SN SVVE+H +I     S N    + +  LPLH+RY  LD+SGY  V    PDLFL  
Sbjct: 197 FRSNRSVVEIHVEIS----SRNYENGEISFKLPLHARYQPLDDSGYSRVEFGEPDLFLCS 256

Query: 181 CNIQ-EKPHNRTCFFRL--ETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCI-- 240
            ++Q ++   R C       +  E   + W IPAG R     VSA+TF +A+ S   I  
Sbjct: 257 SHVQNQRRERRRCLVLSIGRSKTETRSVFWDIPAGIRGHTEYVSALTFAAAVFSAFSILV 316

Query: 241 --VFSTQVRQPKVLKQ 249
             V S++    K LKQ
Sbjct: 317 ASVLSSKGESCKNLKQ 324

[Close]

BLAST of CSPI01G22000 vs. TAIR 10
Match: AT5G46850.3 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic process; LOCATED IN: endomembrane system, endoplasmic reticulum membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: PIG-X/PBN1 (InterPro:IPR013233). )

HSP 1 Score: 151.4 bits (381), Expect = 9.8e-37
Identity = 104/256 (40.62%), Postives = 135/256 (52.73%), Query Frame = 0


Query: 1   MESYYEKHESFIDSLFDDFLTRDLPVNICEVLPSDLNTLLSLSIWSRIKGDGSHRQLSST 60
           MESY+  + S  DS F +FL      +      S++    S      I G+GSHR L ++
Sbjct: 51  MESYFTTYTSLDDSEFRNFLLLQQCSDKVPTQDSNIVPATSFIQHRNITGEGSHRNLITS 110

Query: 61  IKFNTEKSLFQVPTHHCKFIIIERLPSGVFVDPFELEHLLHRAVFSDVAVFGDTNLELPS 120
           IK     S  Q      + +I+ERLP GVF DPFEL+ L  R  FSDV+VFGDTNLELPS
Sbjct: 111 IKLLLHHSESQFR----ELVIVERLPLGVFADPFELQSLQQRRAFSDVSVFGDTNLELPS 170

Query: 121 VLSNISVVEVHKDIGLNILSHNKNLIDFTVDLPLHSRYPRLDESGYVEVRLKAPDLFL-Q 180
             SN SVVE+H +I     S N    + +  LPLH+RY  LD+SGY  V    PDLFL  
Sbjct: 171 FRSNRSVVEIHVEIS----SRNYENGEISFKLPLHARYQPLDDSGYSRVEFGEPDLFLCS 230

Query: 181 CNIQ-EKPHNRTCFFRL--ETDDEEADLTWSIPAGKRSDAGVVSAITFVSALLSVLCI-- 240
            ++Q ++   R C       +  E   + W IPAG R     VSA+TF +A+ S   I  
Sbjct: 231 SHVQNQRRERRRCLVLSIGRSKTETRSVFWDIPAGIRGHTEYVSALTFAAAVFSAFSILV 290

Query: 241 --VFSTQVRQPKVLKQ 249
             V S++    K LKQ
Sbjct: 291 ASVLSSKGESCKNLKQ 298

The following BLAST results are available for this feature:

BLAST of CSPI01G22000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8TBF5	1.7e-09	32.61	Phosphatidylinositol-glycan biosynthesis class X protein OS=Homo sapiens OX=9606...	[more]
Q60GF7	2.2e-09	30.39	Phosphatidylinositol-glycan biosynthesis class X protein OS=Rattus norvegicus OX...	[more]
Q99LV7	8.4e-09	29.41	Phosphatidylinositol-glycan biosynthesis class X protein OS=Mus musculus OX=1009...	[more]

BLAST of CSPI01G22000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LV34	2.2e-124	97.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G470340 PE=3 SV=1	[more]
A0A1S3B562	2.2e-124	91.39	phosphatidylinositol-glycan biosynthesis class X protein isoform X1 OS=Cucumis m...	[more]
A0A1S3B564	2.2e-124	91.39	phosphatidylinositol-glycan biosynthesis class X protein isoform X2 OS=Cucumis m...	[more]
A0A5D3BTA5	4.8e-116	90.83	Phosphatidylinositol-glycan biosynthesis class X protein isoform X2 OS=Cucumis m...	[more]
A0A5A7TCL5	6.3e-116	90.83	Phosphatidylinositol-glycan biosynthesis class X protein isoform X1 OS=Cucumis m...	[more]

BLAST of CSPI01G22000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011657626.2	8.7e-136	98.39	phosphatidylinositol-glycan biosynthesis class X protein isoform X1 [Cucumis sat...	[more]
XP_031736304.1	8.7e-136	98.39	phosphatidylinositol-glycan biosynthesis class X protein isoform X2 [Cucumis sat...	[more]
XP_008442206.1	4.5e-124	91.39	PREDICTED: phosphatidylinositol-glycan biosynthesis class X protein isoform X1 [...	[more]
XP_008442207.1	4.5e-124	91.39	PREDICTED: phosphatidylinositol-glycan biosynthesis class X protein isoform X2 [...	[more]
XP_038881271.1	4.8e-118	85.94	phosphatidylinositol-glycan biosynthesis class X protein isoform X2 [Benincasa h...	[more]

BLAST of CSPI01G22000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46850.1	9.8e-37	40.63	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]
AT5G46850.2	9.8e-37	40.63	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic p...	[more]
AT5G46850.3	9.8e-37	40.63	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: GPI anchor biosynthetic p...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013233	Glycosylphosphatidylinositol-mannosyltransferase I, PIG-X/PBN1	SMART	SM00780	pig_x_1	coord: 49..242 e-value: 1.2E-8 score: 20.6
IPR013233	Glycosylphosphatidylinositol-mannosyltransferase I, PIG-X/PBN1	PFAM	PF08320	PIG-X	coord: 52..236 e-value: 1.9E-27 score: 96.5
IPR040039	Phosphatidylinositol-glycan biosynthesis class X protein	PANTHER	PTHR28650	PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS X PROTEIN	coord: 16..245

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G22000.1	CSPI01G22000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006338	chromatin remodeling
biological_process	GO:0006506	GPI anchor biosynthetic process
biological_process	GO:0034721	histone H3-K4 demethylation, trimethyl-H3-K4-specific
biological_process	GO:0010468	regulation of gene expression
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0034647	histone H3-tri/di/monomethyl-lysine-4 demethylase activity