CSPI01G21160 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G21160
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
LocationChr1: 16695258 .. 16697350 (+)
RNA-Seq ExpressionCSPI01G21160
SyntenyCSPI01G21160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTTAACAAACTTTAATTCTCCTATAACTGGTGTTGCCCATGGGAAGAAACCTGAAAAATTTGGTGGTGTTGATTTCAAACGTTGGCAACAAAAAATGTTGTTCTATCTCACCACTTTAAATTTGGCAAAGTTTCTCACAGAGGATGCTCCCATTTTACCTGAGGGGGGATCTGACAAAGAAAAAAAACTTGTAGTTGTGCATGGAAACATGCAGAATATTTTTGCAACAATTACATTTTGAATCGGTTAGATAACACATTATACAATGTGTATAGTAGTGTTGATTTGGTCAAAAATCTATTGACTTCATTAGAGAAGAAATACAAAACTGAAGTTGTTGGTTCAAAGAAATTTATCGTTGGAAAATTTCTGGATTACAAAATGGTGGACTTCAAAACTGTAATCAATCAGGTTCAGGAAATTCAGGTAATTCTATATGATATATATGCTGAGAATATGACTTTGAGTGAGTCTTTTCAGGTAGCATCAATAATTGAAAAATTGTCACCCTCATGGAAGGACTTCAAAAATTATCTCAAGCATAAACGCAAAGAGATAAAACTTTAGGAACTTGTGGTCCAACTTGGGATTGAAGAAAATAATAGAAAGGCAGAAAAGTGTACTATGGATAATACAATGGATTCCAAGGCCAACATTGTGGAAAATAGATCACAAAGTAACAAGAAAAGGAAGTTTTTTGGTGAAGGTTCAGATAAAAAACCACGATTCACAAAATAGTTCAATGGCAAGTGTTACAACTGCAACAAAATGGGACATCAATCAAAAGATTGTCGTAAGCCAAAGAACTTTAAAAAAAAACATGCCCAAGCTCATATCACAGAAGTTGATGAAGTATCAGATGGTGTTGCAAATATTGACCTTTGTGCAGTCATTTTAGAATGCAACATGGTGGACAATTCGAAGGAGTGGTGGGTAGACACTGGGGCTACTCGTCATATTTGTGCCAACAAGGATATGTTCACATCATATGTGCCAGTTTCTAGTGGATAACAACTATTTATGGGTAACTCCTCTACTTCAAAGGTTGAAGGACAAGGCAAAGTGATTCTTAAGATGACCTCTGGCAAGGAACTCACTCTCAACAATGCGCTTCATGTTCCTAACATTCGCAAGAACTTAGTTTCTGGTTCATTGCTTAGTAAGAATGGCTTTAAGTTGGTTTTTGTATCTAATAAGTTTGTACTTTCCAAGAATGAGATGTACATTGGAAAGGGTTATTTGAGTGATGGTCTATTTAAATTAAATGTACTCACAGTTGTACACAAAAGTATTATTAATAAGGTATCTACTCTTGCTTATATTGTTGAGTCATTTGTTTGGCATGGCAGACTAGGACATGTTAATTTTAATTCTTTGCAAAGACTAATTAATATGAACTTGATTCCAAAATTCACTTTTGACACAAATCATGGATGTGAGATGTGTGTGGAATAAAAAATGACTAAAACACCTTTTCATTCAACTCAGAGAATTACCAAACCTTTAGAGTTAATTCATAGTGATATTTGTGACTTGAAATTTGTGCAAACTAGATGTGGAAAAAAGTATTTTATTACTTTTATCGATGATAGCACAAGATATTGTTATGTTTATCTGCTAAAAAGCAAAGATGAGGCAATTGAAGTGTTTAAGCTTTATAAAAAAGAGGTTGAAAATCAACTTAGCACAAAAATTAAGGCAATAAGAAGTGATCGAGGTGGTGAATATGGTCCTCCTTTTGAACAATTTTGTTCAGAACATGGCATTATTCACCAAACTACAGCTCCTTACTCATCTCAATCGAATGAAATTGTTGAACGAAAAAACCGACCACTTAAGGAAATGATGAACGCAACGCTTATAAGTTCAGGTTTACCCCAAAATTTGTGGGAAGAAGCTTTGTTAACAGCAAATTATTTATTAAACAGAATTCCTCATAAGAAGTCACAAAATATTCCTTATGAAAAGTGGAAAGGAAGAAAACCTTCATATAAATTCTTAAAAGTATGGGGGTGCCTAACAAAGGTTGTCATGCCTAAACCTAAAATGGTTAACATTGGAACAAAAACTGTTGATTGGTTATGCTAG

mRNA sequence

ATGACTTTAACAAACTTTAATTCTCCTATAACTGGTGTTGCCCATGGGAAGAAACCTGAAAAATTTGGTGGTGTTGATTTCAAACGTTGGCAACAAAAAATGTTGTTCTATCTCACCACTTTAAATTTGGCAAAGTTTCTCACAGAGGATGCTCCCATTTTACCTGAGGGGGGATCTGACAAAGAAAAAAAACTTAAGAAATACAAAACTGAAGTTGTTGGTTCAAAGAAATTTATCGTTGGAAAATTTCTGGATTACAAAATGGTGGACTTCAAAACTGTAATCAATCAGGTTCAGGAAATTCAGGTAATTCTATATGATATATATGCTGAGAATATGACTTTGAGTGAGTCTTTTCAGGAACTTGTGGTCCAACTTGGGATTGAAGAAAATAATAGAAAGGCAGAAAAGTGTACTATGGATAATACAATGGATTCCAAGGCCAACATTGTGGAAAATAGATCACAAAGTAACAAGAAAAGGAAGTTTTTTGGTGAAGAAGTTGATGAAGTATCAGATGGTGTTGCAAATATTGACCTTTGTGCAGTCATTTTAGAATGCAACATGGTGGACAATTCGAAGGAGTGGTGGGTAGACACTGGGGCTACTCGTCATATTTGTGCCAACAAGGATATGTTCACATCATATGTGCCAGTTGAAGGACAAGGCAAAGTGATTCTTAAGATGACCTCTGGCAAGGAACTCACTCTCAACAATGCGCTTCATGTTCCTAACATTCGCAAGAACTTAGTTTCTGGTTCATTGCTTAGTAAGAATGGCTTTAAGTTGGTTTTTGTATCTAATAAGTTTGTACTTTCCAAGAATGAGATGTACATTGGAAAGGGTTATTTGAGTGATGGTCTATTTAAATTAAATGTACTCACAGTTGTACACAAAAGTATTATTAATAAGGTATCTACTCTTGCTTATATTGTTGAGTCATTTGTTTGGCATGGCAGACTAGGACATGTTAATTTTAATTCTTTGCAAAGACTAATTAATATGAACTTGATTCCAAAATTCACTTTTGACACAAATCATGGATGTGAGATGTGTAGAATTACCAAACCTTTAGAGTTAATTCATAGTGATATTTGTGACTTGAAATTTGTGCAAACTAGATGTGGAAAAAAGTATTTTATTACTTTTATCGATGATAGCACAAGATATTGTTATGTTTATCTGCTAAAAAGCAAAGATGAGGCAATTGAAGTGTTTAAGCTTTATAAAAAAGAGGTTGAAAATCAACTTAGCACAAAAATTAAGGCAATAAGAAGTGATCGAGGTGGTGAATATGGTCCTCCTTTTGAACAATTTTGTTCAGAACATGGCATTATTCACCAAACTACAGCTCCTTACTCATCTCAATCGAATGAAATTGTTGAACGAAAAAACCGACCACTTAAGGAAATGATGAACGCAACGCTTATAAGTTCAGGTTTACCCCAAAATTTGTGGGAAGAAGCTTTGTTAACAGCAAATTATTTATTAAACAGAATTCCTCATAAGAAGTCACAAAATATTCCTTATGAAAAGTGGAAAGGAAGAAAACCTTCATATAAATTCTTAAAAGTATGGGGGTGCCTAACAAAGGTTGTCATGCCTAAACCTAAAATGGTTAACATTGGAACAAAAACTGTTGATTGGTTATGCTAG

Coding sequence (CDS)

ATGACTTTAACAAACTTTAATTCTCCTATAACTGGTGTTGCCCATGGGAAGAAACCTGAAAAATTTGGTGGTGTTGATTTCAAACGTTGGCAACAAAAAATGTTGTTCTATCTCACCACTTTAAATTTGGCAAAGTTTCTCACAGAGGATGCTCCCATTTTACCTGAGGGGGGATCTGACAAAGAAAAAAAACTTAAGAAATACAAAACTGAAGTTGTTGGTTCAAAGAAATTTATCGTTGGAAAATTTCTGGATTACAAAATGGTGGACTTCAAAACTGTAATCAATCAGGTTCAGGAAATTCAGGTAATTCTATATGATATATATGCTGAGAATATGACTTTGAGTGAGTCTTTTCAGGAACTTGTGGTCCAACTTGGGATTGAAGAAAATAATAGAAAGGCAGAAAAGTGTACTATGGATAATACAATGGATTCCAAGGCCAACATTGTGGAAAATAGATCACAAAGTAACAAGAAAAGGAAGTTTTTTGGTGAAGAAGTTGATGAAGTATCAGATGGTGTTGCAAATATTGACCTTTGTGCAGTCATTTTAGAATGCAACATGGTGGACAATTCGAAGGAGTGGTGGGTAGACACTGGGGCTACTCGTCATATTTGTGCCAACAAGGATATGTTCACATCATATGTGCCAGTTGAAGGACAAGGCAAAGTGATTCTTAAGATGACCTCTGGCAAGGAACTCACTCTCAACAATGCGCTTCATGTTCCTAACATTCGCAAGAACTTAGTTTCTGGTTCATTGCTTAGTAAGAATGGCTTTAAGTTGGTTTTTGTATCTAATAAGTTTGTACTTTCCAAGAATGAGATGTACATTGGAAAGGGTTATTTGAGTGATGGTCTATTTAAATTAAATGTACTCACAGTTGTACACAAAAGTATTATTAATAAGGTATCTACTCTTGCTTATATTGTTGAGTCATTTGTTTGGCATGGCAGACTAGGACATGTTAATTTTAATTCTTTGCAAAGACTAATTAATATGAACTTGATTCCAAAATTCACTTTTGACACAAATCATGGATGTGAGATGTGTAGAATTACCAAACCTTTAGAGTTAATTCATAGTGATATTTGTGACTTGAAATTTGTGCAAACTAGATGTGGAAAAAAGTATTTTATTACTTTTATCGATGATAGCACAAGATATTGTTATGTTTATCTGCTAAAAAGCAAAGATGAGGCAATTGAAGTGTTTAAGCTTTATAAAAAAGAGGTTGAAAATCAACTTAGCACAAAAATTAAGGCAATAAGAAGTGATCGAGGTGGTGAATATGGTCCTCCTTTTGAACAATTTTGTTCAGAACATGGCATTATTCACCAAACTACAGCTCCTTACTCATCTCAATCGAATGAAATTGTTGAACGAAAAAACCGACCACTTAAGGAAATGATGAACGCAACGCTTATAAGTTCAGGTTTACCCCAAAATTTGTGGGAAGAAGCTTTGTTAACAGCAAATTATTTATTAAACAGAATTCCTCATAAGAAGTCACAAAATATTCCTTATGAAAAGTGGAAAGGAAGAAAACCTTCATATAAATTCTTAAAAGTATGGGGGTGCCTAACAAAGGTTGTCATGCCTAAACCTAAAATGGTTAACATTGGAACAAAAACTGTTGATTGGTTATGCTAG

Protein sequence

MTLTNFNSPITGVAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSDKEKKLKKYKTEVVGSKKFIVGKFLDYKMVDFKTVINQVQEIQVILYDIYAENMTLSESFQELVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQSNKKRKFFGEEVDEVSDGVANIDLCAVILECNMVDNSKEWWVDTGATRHICANKDMFTSYVPVEGQGKVILKMTSGKELTLNNALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVHKSIINKVSTLAYIVESFVWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMCRITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAIEVFKLYKKEVENQLSTKIKAIRSDRGGEYGPPFEQFCSEHGIIHQTTAPYSSQSNEIVERKNRPLKEMMNATLISSGLPQNLWEEALLTANYLLNRIPHKKSQNIPYEKWKGRKPSYKFLKVWGCLTKVVMPKPKMVNIGTKTVDWLC*
Homology
BLAST of CSPI01G21160 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 9.7e-58
Identity = 128/385 (33.25%), Postives = 201/385 (52.21%), Query Frame = 0

Query: 195 EWWVDTGATRHICANKDMF---------------TSYVPVEGQGKVILKMTSGKELTLNN 254
           EW VDT A+ H    +D+F               TSY  + G G + +K   G  L L +
Sbjct: 293 EWVVDTAASHHATPVRDLFCRYVAGDFGTVKMGNTSYSKIAGIGDICIKTNVGCTLVLKD 352

Query: 255 ALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVHK 314
             HVP++R NL+SG  L ++G++  F + K+ L+K  + I KG     L++ N      +
Sbjct: 353 VRHVPDLRMNLISGIALDRDGYESYFANQKWRLTKGSLVIAKGVARGTLYRTNA-----E 412

Query: 315 SIINKVSTLAYIVESFVWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMCRITKP-- 374
               +++     +   +WH R+GH++   LQ L   +LI      T   C+ C   K   
Sbjct: 413 ICQGELNAAQDEISVDLWHKRMGHMSEKGLQILAKKSLISYAKGTTVKPCDYCLFGKQHR 472

Query: 375 -------------LELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAIE 434
                        L+L++SD+C    +++  G KYF+TFIDD++R  +VY+LK+KD+  +
Sbjct: 473 VSFQTSSERKLNILDLVYSDVCGPMEIESMGGNKYFVTFIDDASRKLWVYILKTKDQVFQ 532

Query: 435 VFKLYKKEVENQLSTKIKAIRSDRGGEY-GPPFEQFCSEHGIIHQTTAPYSSQSNEIVER 494
           VF+ +   VE +   K+K +RSD GGEY    FE++CS HGI H+ T P + Q N + ER
Sbjct: 533 VFQKFHALVERETGRKLKRLRSDNGGEYTSREFEEYCSSHGIRHEKTVPGTPQHNGVAER 592

Query: 495 KNRPLKEMMNATLISSGLPQNLWEEALLTANYLLNRIPH-KKSQNIPYEKWKGRKPSYKF 548
            NR + E + + L  + LP++ W EA+ TA YL+NR P    +  IP   W  ++ SY  
Sbjct: 593 MNRTIVEKVRSMLRMAKLPKSFWGEAVQTACYLINRSPSVPLAFEIPERVWTNKEVSYSH 652

BLAST of CSPI01G21160 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.5e-37
Identity = 111/381 (29.13%), Postives = 182/381 (47.77%), Query Frame = 0

Query: 185 LECNMVDNSKEWWVDTGATRHICANKDMFTSYVPVEGQGKVILK---------------M 244
           L  N   N+  W +D+GAT HI ++ +  + + P  G   V++                 
Sbjct: 299 LAVNSPYNANNWLLDSGATHHITSDFNNLSFHQPYTGGDDVMIADGSTIPITHTGSASLP 358

Query: 245 TSGKELTLNNALHVPNIRKNLVS-GSLLSKNGFKLVFVSNKFVLS--KNEMYIGKGYLSD 304
           TS + L LN  L+VPNI KNL+S   L + N   + F    F +      + + +G   D
Sbjct: 359 TSSRSLDLNKVLYVPNIHKNLISVYRLCNTNRVSVEFFPASFQVKDLNTGVPLLQGKTKD 418

Query: 305 GLFKLNVLTVVHKSIINKVSTLAYIVESFVWHGRLGHVNFNSLQRLINMNLIPKFTFDTN 364
            L++     +     ++  ++         WH RLGH +   L  +I+ + +P    + +
Sbjct: 419 ELYE---WPIASSQAVSMFASPCSKATHSSWHSRLGHPSLAILNSVISNHSLP--VLNPS 478

Query: 365 H---GCEMCRI---------------TKPLELIHSDICDLKFVQTRCGKKYFITFIDDST 424
           H    C  C I               +KPLE I+SD+     +      +Y++ F+D  T
Sbjct: 479 HKLLSCSDCFINKSHKVPFSNSTITSSKPLEYIYSDVWSSPILSID-NYRYYVIFVDHFT 538

Query: 425 RYCYVYLLKSKDEAIEVFKLYKKEVENQLSTKIKAIRSDRGGEYGPPFEQFCSEHGIIHQ 484
           RY ++Y LK K +  + F ++K  VEN+  T+I  + SD GGE+      + S+HGI H 
Sbjct: 539 RYTWLYPLKQKSQVKDTFIIFKSLVENRFQTRIGTLYSDNGGEF-VVLRDYLSQHGISHF 598

Query: 485 TTAPYSSQSNEIVERKNRPLKEMMNATLISSGLPQNLWEEALLTANYLLNRIPHKKSQ-N 529
           T+ P++ + N + ERK+R + EM    L  + +P+  W  A   A YL+NR+P    Q  
Sbjct: 599 TSPPHTPEHNGLSERKHRHIVEMGLTLLSHASVPKTYWPYAFSVAVYLINRLPTPLLQLQ 658

BLAST of CSPI01G21160 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.7e-36
Identity = 113/377 (29.97%), Postives = 179/377 (47.48%), Query Frame = 0

Query: 192 NSKEWWVDTGATRHICANKDMFTSYVPVEGQGKVILK---------------MTSGKELT 251
           +S  W +D+GAT HI ++ +  + + P  G   V++                 T  + L 
Sbjct: 327 SSNNWLLDSGATHHITSDFNNLSLHQPYTGGDDVMVADGSTIPISHTGSTSLSTKSRPLN 386

Query: 252 LNNALHVPNIRKNLVS-GSLLSKNGFKLVFVSNKFVLS--KNEMYIGKGYLSDGLFKLNV 311
           L+N L+VPNI KNL+S   L + NG  + F    F +      + + +G   D L++  +
Sbjct: 387 LHNILYVPNIHKNLISVYRLCNANGVSVEFFPASFQVKDLNTGVPLLQGKTKDELYEWPI 446

Query: 312 LTVVHKSIINKVSTLAYIVESFVWHGRLGHVNFNSLQRLIN------MNLIPKFTFDTNH 371
            +    S+    S+ A       WH RLGH   + L  +I+      +N   KF      
Sbjct: 447 ASSQPVSLFASPSSKA---THSSWHARLGHPAPSILNSVISNYSLSVLNPSHKFL----- 506

Query: 372 GCEMCRI---------------TKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCY 431
            C  C I               T+PLE I+SD+     + +    +Y++ F+D  TRY +
Sbjct: 507 SCSDCLINKSNKVPFSQSTINSTRPLEYIYSDVWSSP-ILSHDNYRYYVIFVDHFTRYTW 566

Query: 432 VYLLKSKDEAIEVFKLYKKEVENQLSTKIKAIRSDRGGEYGPPFEQFCSEHGIIHQTTAP 491
           +Y LK K +  E F  +K  +EN+  T+I    SD GGE+   +E F S+HGI H T+ P
Sbjct: 567 LYPLKQKSQVKETFITFKNLLENRFQTRIGTFYSDNGGEFVALWEYF-SQHGISHLTSPP 626

Query: 492 YSSQSNEIVERKNRPLKEMMNATLISSGLPQNLWEEALLTANYLLNRIPHKKSQ-NIPYE 529
           ++ + N + ERK+R + E     L  + +P+  W  A   A YL+NR+P    Q   P++
Sbjct: 627 HTPEHNGLSERKHRHIVETGLTLLSHASIPKTYWPYAFAVAVYLINRLPTPLLQLESPFQ 686

BLAST of CSPI01G21160 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 151.0 bits (380), Expect = 4.0e-35
Identity = 116/429 (27.04%), Postives = 193/429 (44.99%), Query Frame = 0

Query: 150 IVENRSQSNKKRKFFGEEVDEVSDGVA----NIDLCAVILECNMVDNSKEWWVDTGATRH 209
           I+ N+++ N+K     +     S G+A     ++  +V+  C  V       +D+GA+ H
Sbjct: 251 ILNNKNKENEK-----QVQTATSHGIAFMVKEVNNTSVMDNCGFV-------LDSGASDH 310

Query: 210 ICANKDMFTSYVPVEGQGKV---------------ILKMTSGKELTLNNALHVPNIRKNL 269
           +  ++ ++T  V V    K+               I+++ +  E+TL + L       NL
Sbjct: 311 LINDESLYTDSVEVVPPLKIAVAKQGEFIYATKRGIVRLRNDHEITLEDVLFCKEAAGNL 370

Query: 270 VSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVHKSIINKVSTLAY 329
           +S   L + G  + F  +   +SKN + + K   + G+  LN + V++       S  A 
Sbjct: 371 MSVKRLQEAGMSIEFDKSGVTISKNGLMVVK---NSGM--LNNVPVIN---FQAYSINAK 430

Query: 330 IVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTN--HGCEMC--------------- 389
              +F +WH R GH++   L  +   N+    +   N    CE+C               
Sbjct: 431 HKNNFRLWHERFGHISDGKLLEIKRKNMFSDQSLLNNLELSCEICEPCLNGKQARLPFKQ 490

Query: 390 -----RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAIEVFK 449
                 I +PL ++HSD+C      T   K YF+ F+D  T YC  YL+K K +   +F+
Sbjct: 491 LKDKTHIKRPLFVVHSDVCGPITPVTLDDKNYFVIFVDQFTHYCVTYLIKYKSDVFSMFQ 550

Query: 450 LYKKEVENQLSTKIKAIRSDRGGEY-GPPFEQFCSEHGIIHQTTAPYSSQSNEIVERKNR 509
            +  + E   + K+  +  D G EY      QFC + GI +  T P++ Q N + ER  R
Sbjct: 551 DFVAKSEAHFNLKVVYLYIDNGREYLSNEMRQFCVKKGISYHLTVPHTPQLNGVSERMIR 610

Query: 510 PLKEMMNATLISSGLPQNLWEEALLTANYLLNRIPHK---KSQNIPYEKWKGRKPSYKFL 533
            + E     +  + L ++ W EA+LTA YL+NRIP +    S   PYE W  +KP  K L
Sbjct: 611 TITEKARTMVSGAKLDKSFWGEAVLTATYLINRIPSRALVDSSKTPYEMWHNKKPYLKHL 659

BLAST of CSPI01G21160 vs. ExPASy Swiss-Prot
Match: Q12491 (Transposon Ty2-B Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY2B-B PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 5.8e-18
Identity = 81/330 (24.55%), Postives = 137/330 (41.52%), Query Frame = 0

Query: 217 VPVEGQGKVILKMTSGKELTLNNALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNE 276
           +P+   G +     +G + ++  ALH PNI  +L+S S L+       F  N    S   
Sbjct: 490 IPINAIGNLHFNFQNGTKTSI-KALHTPNIAYDLLSLSELANQNITACFTRNTLERSDGT 549

Query: 277 M-----------YIGKGYL-SDGLFKLNVLTVVHKSIINKVSTLAYIVESFVWHGRLGHV 336
           +           ++ K YL    + KL +  V     +NK           + H  LGH 
Sbjct: 550 VLAPIVKHGDFYWLSKKYLIPSHISKLTINNVNKSKSVNKYPYP-------LIHRMLGHA 609

Query: 337 NFNSLQRLINMNLIP-------KFTFDTNHGCEMCRITK-------------------PL 396
           NF S+Q+ +  N +        +++  + + C  C I K                   P 
Sbjct: 610 NFRSIQKSLKKNAVTYLKESDIEWSNASTYQCPDCLIGKSTKHRHVKGSRLKYQESYEPF 669

Query: 397 ELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLL--KSKDEAIEVFKLYKKEVENQ 456
           + +H+DI        +    YFI+F D+ TR+ +VY L  + ++  + VF      ++NQ
Sbjct: 670 QYLHTDIFGPVHHLPKSAPSYFISFTDEKTRFQWVYPLHDRREESILNVFTSILAFIKNQ 729

Query: 457 LSTKIKAIRSDRGGEY-GPPFEQFCSEHGIIHQTTAPYSSQSNEIVERKNRPLKEMMNAT 506
            + ++  I+ DRG EY      +F +  GI    T    S+++ + ER NR L       
Sbjct: 730 FNARVLVIQMDRGSEYTNKTLHKFFTNRGITACYTTTADSRAHGVAERLNRTLLNDCRTL 789

BLAST of CSPI01G21160 vs. ExPASy TrEMBL
Match: A0A438E836 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_2120 PE=4 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 4.7e-188
Identity = 357/648 (55.09%), Postives = 447/648 (68.98%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 35  VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 94

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE  G+KKF+VG+FLDYKMVD
Sbjct: 95  SDFLCRNYVMNGLADSLYNVYSDKKTAKELWESLDRKYKTEDAGAKKFVVGRFLDYKMVD 154

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 155 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 214

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQSNKK------RKFFGEE---VDEVS 252
           L+++L IEE+NR+ EK       ++KAN VE+  QS+K       +K   +E   +D+++
Sbjct: 215 LIIRLRIEEDNRRFEKKGAHTLNEAKANFVEH-GQSHKSVDCRLPKKNKPKEANVIDDIT 274

Query: 253 DGVANIDLCAVILECNMV-DNSKEWWVDTGATRHICANKDMFTSYVPVE----------- 312
             V++IDL AV+ E N+V  N KEWW+DTGATRH+C++K MF+++ P+E           
Sbjct: 275 KNVSDIDLTAVVSEVNLVGSNPKEWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSA 334

Query: 313 -----GQGKVILKMTSGKELTLNNALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKN 372
                GQGKVILKMTSGKELTL N L+VP IRKNLVSGSLL+ +GF+LVF SNKF+LSK+
Sbjct: 335 TSEIKGQGKVILKMTSGKELTLTNVLYVPEIRKNLVSGSLLNNHGFRLVFESNKFILSKS 394

Query: 373 EMYIGKGYLSDGLFKLNVLTVVHKSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLIN 432
            MY+GKGY+SDG++KLNV+ ++ KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN
Sbjct: 395 GMYVGKGYMSDGMWKLNVMAII-KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLIN 454

Query: 433 MNLIPKFTFDTNHGCEMC--------------RITKPLELIHSDICDLKFVQTRCGKKYF 492
           +N IP F  ++NH CE C              R T+PL+LIHSDICDLKFVQTR G KYF
Sbjct: 455 LNHIPTFQINSNHKCETCAEAKLTRSSFQSVERNTEPLDLIHSDICDLKFVQTRGGNKYF 514

Query: 493 ITFIDDSTRYCYVYLLKSKDEAIEVFKLYKKEVENQLSTKIKAIRSDRGGEYGPPFEQFC 549
           ITF+DDST+YCYVYLLKSKDEAIE F LYK EVENQL+ KIK +RSDRGGEY  PF   C
Sbjct: 515 ITFVDDSTKYCYVYLLKSKDEAIEKFVLYKNEVENQLNKKIKVLRSDRGGEYESPFVDIC 574

BLAST of CSPI01G21160 vs. ExPASy TrEMBL
Match: A0A438K0G8 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_784 PE=4 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 5.7e-186
Identity = 360/685 (52.55%), Postives = 448/685 (65.40%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 35  VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 94

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE  G+KKF+VG+FLDYKMVD
Sbjct: 95  SDFLCRNYVMNGLVDSLYNVYSDKKTAKELWESLDRKYKTEDAGAKKFVVGRFLDYKMVD 154

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 155 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 214

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQS--------------------NKKR 252
           L+++L IEE+NR++EK    N  ++KAN VE+   S                    +KK 
Sbjct: 215 LIIRLRIEEDNRRSEKKGAHNLNEAKANFVEHGQSSKAKTNNNKGKGSKLGPKGGISKKP 274

Query: 253 KFFGE--------------------------EVDEVSDGVANIDLCAVILECNMV-DNSK 312
           KF G+                           +D+++  V +IDL AV+ E N+V  N K
Sbjct: 275 KFQGKCFNCGKQGHKSVDCRLPKKNKPKEANVIDDITKNVYDIDLTAVVSEVNLVGSNPK 334

Query: 313 EWWVDTGATRHICANKDMFTSYVPVE----------------GQGKVILKMTSGKELTLN 372
           EWW+DTGATRH+C++K MF+++ P+E                GQGKVILKMTSGKELTL 
Sbjct: 335 EWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLT 394

Query: 373 NALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVH 432
           N L+VP IRKNLVSGSLL+ +GF+LVF SNK VLSK+ MY+GKGY+SDG++KLNV+T++ 
Sbjct: 395 NVLYVPEIRKNLVSGSLLNNHGFRLVFDSNKVVLSKSGMYVGKGYMSDGMWKLNVMTII- 454

Query: 433 KSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMC----- 492
           KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN+N IP F  ++NH CE C     
Sbjct: 455 KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLINLNHIPTFQINSNHKCETCVEAKL 514

Query: 493 ---------RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAI 549
                    R T+PL+LIHSDICDLKFVQTR G KYFITF+DDST+YCYVYLLKSKDEAI
Sbjct: 515 TRSSFQSVERNTEPLDLIHSDICDLKFVQTRGGNKYFITFVDDSTKYCYVYLLKSKDEAI 574

BLAST of CSPI01G21160 vs. ExPASy TrEMBL
Match: A0A438K1P8 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3292 PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 9.8e-186
Identity = 360/685 (52.55%), Postives = 448/685 (65.40%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 35  VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 94

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE VG+KKF+VG+FLDYKMVD
Sbjct: 95  SDFLCRNYVMNGLADSLYNVYSDKKTAKELWESLDRKYKTEDVGAKKFVVGRFLDYKMVD 154

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 155 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 214

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQS--------------------NKKR 252
           L+++L IEE+NR++EK       ++KAN VE+   S                    +KK 
Sbjct: 215 LIIRLRIEEDNRRSEKKGAHTLNEAKANFVEHGQSSKAKMNNNKGKGSKLGPKGGISKKP 274

Query: 253 KFFGE--------------------------EVDEVSDGVANIDLCAVILECNMV-DNSK 312
           KF G+                           +D+++  V +IDL AV+ E N+V  N K
Sbjct: 275 KFQGKCFNCGKQGHKSVDCRLPKKNKPKEANVIDDITKNVYDIDLTAVVSEVNLVGSNPK 334

Query: 313 EWWVDTGATRHICANKDMFTSYVPVE----------------GQGKVILKMTSGKELTLN 372
           EWW+DTGATRH+C++K MF+++ P+E                GQGKVILKMTSGKELTL 
Sbjct: 335 EWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLT 394

Query: 373 NALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVH 432
           N L+VP IRKNLVSGSLL+ +GF+LVF SNK VLSK+ MY+GKGY+SDG++KLNV+T++ 
Sbjct: 395 NVLYVPEIRKNLVSGSLLNNHGFRLVFDSNKVVLSKSGMYVGKGYMSDGMWKLNVMTII- 454

Query: 433 KSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMC----- 492
           KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN+N IP F  ++NH CE C     
Sbjct: 455 KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLINLNHIPTFQINSNHKCETCVEAKL 514

Query: 493 ---------RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAI 549
                    R T+PL+LIHSDICDLKFVQTR G KYFITF+DDST+YCYVYLLKSKDEAI
Sbjct: 515 TRSSFQIVERNTEPLDLIHSDICDLKFVQTRGGNKYFITFVDDSTKYCYVYLLKSKDEAI 574

BLAST of CSPI01G21160 vs. ExPASy TrEMBL
Match: A5AEN5 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_011340 PE=4 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.7e-185
Identity = 359/685 (52.41%), Postives = 447/685 (65.26%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 35  VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 94

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE  G+KKF+VG+FLDYKMVD
Sbjct: 95  SDFLCRNYVMNGLADSLYNVYSDKKTAKELWESLDRKYKTEDAGAKKFVVGRFLDYKMVD 154

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 155 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 214

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQS--------------------NKKR 252
           L+++L IEE+NR++EK       ++KAN VE+   S                    +KK 
Sbjct: 215 LIIRLRIEEDNRRSEKKGAHTLNEAKANFVEHGQSSKAKTNNNKGKGSKLGPKGGISKKP 274

Query: 253 KFFGE--------------------------EVDEVSDGVANIDLCAVILECNMV-DNSK 312
           KF G+                           +D+++  V +IDL AV+ E N+V  N K
Sbjct: 275 KFQGKCFNCGKQGHKSVDCRLPKKNKPKEANVIDDITKNVYDIDLTAVVSEVNLVGSNPK 334

Query: 313 EWWVDTGATRHICANKDMFTSYVPVE----------------GQGKVILKMTSGKELTLN 372
           EWW+DTGATRH+C++K MF+++ P+E                GQGKVILKMTSGKELTL 
Sbjct: 335 EWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLT 394

Query: 373 NALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVH 432
           N L+VP IRKNLVSGSLL+ +GF+LVF SNK VLSK+ MY+GKGY+SDG++KLNV+T++ 
Sbjct: 395 NVLYVPEIRKNLVSGSLLNNHGFRLVFXSNKVVLSKSGMYVGKGYMSDGMWKLNVMTII- 454

Query: 433 KSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMC----- 492
           KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN+N IP F  ++NH CE C     
Sbjct: 455 KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLINLNHIPTFQINSNHKCETCVEAKL 514

Query: 493 ---------RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAI 549
                    R T+PL+LIHSDICDLKFVQTR G KYFITF+DDST+YCYVYLLKSKDEAI
Sbjct: 515 TRSSFQSVERNTEPLDLIHSDICDLKFVQTRGGNKYFITFVDDSTKYCYVYLLKSKDEAI 574

BLAST of CSPI01G21160 vs. ExPASy TrEMBL
Match: A0A438IVT6 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_4199 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 2.2e-185
Identity = 359/685 (52.41%), Postives = 447/685 (65.26%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 9   VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 68

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE  G+KKF+VG+FLDYKMVD
Sbjct: 69  SDFLCRNYVMNGLADSLYNVYSDKKTAKELWESLDRKYKTEDAGAKKFVVGRFLDYKMVD 128

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 129 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 188

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQS--------------------NKKR 252
           L+++L IEE+NR++EK       ++KAN VE+   S                    +KK 
Sbjct: 189 LIIRLRIEEDNRRSEKKGAHTLNEAKANFVEHGQSSKAKTNNNKGKGSKLGPKGGISKKP 248

Query: 253 KFFGE--------------------------EVDEVSDGVANIDLCAVILECNMV-DNSK 312
           KF G+                           +D+++  V +IDL AV+ E N+V  N K
Sbjct: 249 KFQGKCFNCGKQGHKSVDCRLPKKNKPKEANVIDDITKNVYDIDLTAVVSEVNLVGSNPK 308

Query: 313 EWWVDTGATRHICANKDMFTSYVPVE----------------GQGKVILKMTSGKELTLN 372
           EWW+DTGATRH+C++K MF+++ P+E                GQGKVILKMTSGKELTL 
Sbjct: 309 EWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLT 368

Query: 373 NALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVH 432
           N L+VP IRKNLVSGSLL+ +GF+LVF SNK VLSK+ MY+GKGY+SDG++KLNV+T++ 
Sbjct: 369 NVLYVPEIRKNLVSGSLLNNHGFRLVFESNKVVLSKSGMYVGKGYMSDGMWKLNVMTII- 428

Query: 433 KSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMC----- 492
           KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN+N IP F  ++NH CE C     
Sbjct: 429 KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLINLNHIPTFQINSNHKCETCVEAKL 488

Query: 493 ---------RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAI 549
                    R T+PL+LIHSDICDLKFVQTR G KYFITF+DDST+YCYVYLLKSKDEAI
Sbjct: 489 TRSSFQSVERNTEPLDLIHSDICDLKFVQTRGGNKYFITFVDDSTKYCYVYLLKSKDEAI 548

BLAST of CSPI01G21160 vs. NCBI nr
Match: RVW43863.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 667.5 bits (1721), Expect = 9.7e-188
Identity = 357/648 (55.09%), Postives = 447/648 (68.98%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 35  VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 94

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE  G+KKF+VG+FLDYKMVD
Sbjct: 95  SDFLCRNYVMNGLADSLYNVYSDKKTAKELWESLDRKYKTEDAGAKKFVVGRFLDYKMVD 154

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 155 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 214

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQSNKK------RKFFGEE---VDEVS 252
           L+++L IEE+NR+ EK       ++KAN VE+  QS+K       +K   +E   +D+++
Sbjct: 215 LIIRLRIEEDNRRFEKKGAHTLNEAKANFVEH-GQSHKSVDCRLPKKNKPKEANVIDDIT 274

Query: 253 DGVANIDLCAVILECNMV-DNSKEWWVDTGATRHICANKDMFTSYVPVE----------- 312
             V++IDL AV+ E N+V  N KEWW+DTGATRH+C++K MF+++ P+E           
Sbjct: 275 KNVSDIDLTAVVSEVNLVGSNPKEWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSA 334

Query: 313 -----GQGKVILKMTSGKELTLNNALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKN 372
                GQGKVILKMTSGKELTL N L+VP IRKNLVSGSLL+ +GF+LVF SNKF+LSK+
Sbjct: 335 TSEIKGQGKVILKMTSGKELTLTNVLYVPEIRKNLVSGSLLNNHGFRLVFESNKFILSKS 394

Query: 373 EMYIGKGYLSDGLFKLNVLTVVHKSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLIN 432
            MY+GKGY+SDG++KLNV+ ++ KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN
Sbjct: 395 GMYVGKGYMSDGMWKLNVMAII-KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLIN 454

Query: 433 MNLIPKFTFDTNHGCEMC--------------RITKPLELIHSDICDLKFVQTRCGKKYF 492
           +N IP F  ++NH CE C              R T+PL+LIHSDICDLKFVQTR G KYF
Sbjct: 455 LNHIPTFQINSNHKCETCAEAKLTRSSFQSVERNTEPLDLIHSDICDLKFVQTRGGNKYF 514

Query: 493 ITFIDDSTRYCYVYLLKSKDEAIEVFKLYKKEVENQLSTKIKAIRSDRGGEYGPPFEQFC 549
           ITF+DDST+YCYVYLLKSKDEAIE F LYK EVENQL+ KIK +RSDRGGEY  PF   C
Sbjct: 515 ITFVDDSTKYCYVYLLKSKDEAIEKFVLYKNEVENQLNKKIKVLRSDRGGEYESPFVDIC 574

BLAST of CSPI01G21160 vs. NCBI nr
Match: RVX14679.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 660.6 bits (1703), Expect = 1.2e-185
Identity = 360/685 (52.55%), Postives = 448/685 (65.40%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 35  VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 94

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE  G+KKF+VG+FLDYKMVD
Sbjct: 95  SDFLCRNYVMNGLVDSLYNVYSDKKTAKELWESLDRKYKTEDAGAKKFVVGRFLDYKMVD 154

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 155 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 214

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQS--------------------NKKR 252
           L+++L IEE+NR++EK    N  ++KAN VE+   S                    +KK 
Sbjct: 215 LIIRLRIEEDNRRSEKKGAHNLNEAKANFVEHGQSSKAKTNNNKGKGSKLGPKGGISKKP 274

Query: 253 KFFGE--------------------------EVDEVSDGVANIDLCAVILECNMV-DNSK 312
           KF G+                           +D+++  V +IDL AV+ E N+V  N K
Sbjct: 275 KFQGKCFNCGKQGHKSVDCRLPKKNKPKEANVIDDITKNVYDIDLTAVVSEVNLVGSNPK 334

Query: 313 EWWVDTGATRHICANKDMFTSYVPVE----------------GQGKVILKMTSGKELTLN 372
           EWW+DTGATRH+C++K MF+++ P+E                GQGKVILKMTSGKELTL 
Sbjct: 335 EWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLT 394

Query: 373 NALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVH 432
           N L+VP IRKNLVSGSLL+ +GF+LVF SNK VLSK+ MY+GKGY+SDG++KLNV+T++ 
Sbjct: 395 NVLYVPEIRKNLVSGSLLNNHGFRLVFDSNKVVLSKSGMYVGKGYMSDGMWKLNVMTII- 454

Query: 433 KSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMC----- 492
           KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN+N IP F  ++NH CE C     
Sbjct: 455 KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLINLNHIPTFQINSNHKCETCVEAKL 514

Query: 493 ---------RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAI 549
                    R T+PL+LIHSDICDLKFVQTR G KYFITF+DDST+YCYVYLLKSKDEAI
Sbjct: 515 TRSSFQSVERNTEPLDLIHSDICDLKFVQTRGGNKYFITFVDDSTKYCYVYLLKSKDEAI 574

BLAST of CSPI01G21160 vs. NCBI nr
Match: RVX15136.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 659.8 bits (1701), Expect = 2.0e-185
Identity = 360/685 (52.55%), Postives = 448/685 (65.40%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 35  VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 94

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE VG+KKF+VG+FLDYKMVD
Sbjct: 95  SDFLCRNYVMNGLADSLYNVYSDKKTAKELWESLDRKYKTEDVGAKKFVVGRFLDYKMVD 154

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 155 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 214

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQS--------------------NKKR 252
           L+++L IEE+NR++EK       ++KAN VE+   S                    +KK 
Sbjct: 215 LIIRLRIEEDNRRSEKKGAHTLNEAKANFVEHGQSSKAKMNNNKGKGSKLGPKGGISKKP 274

Query: 253 KFFGE--------------------------EVDEVSDGVANIDLCAVILECNMV-DNSK 312
           KF G+                           +D+++  V +IDL AV+ E N+V  N K
Sbjct: 275 KFQGKCFNCGKQGHKSVDCRLPKKNKPKEANVIDDITKNVYDIDLTAVVSEVNLVGSNPK 334

Query: 313 EWWVDTGATRHICANKDMFTSYVPVE----------------GQGKVILKMTSGKELTLN 372
           EWW+DTGATRH+C++K MF+++ P+E                GQGKVILKMTSGKELTL 
Sbjct: 335 EWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLT 394

Query: 373 NALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVH 432
           N L+VP IRKNLVSGSLL+ +GF+LVF SNK VLSK+ MY+GKGY+SDG++KLNV+T++ 
Sbjct: 395 NVLYVPEIRKNLVSGSLLNNHGFRLVFDSNKVVLSKSGMYVGKGYMSDGMWKLNVMTII- 454

Query: 433 KSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMC----- 492
           KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN+N IP F  ++NH CE C     
Sbjct: 455 KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLINLNHIPTFQINSNHKCETCVEAKL 514

Query: 493 ---------RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAI 549
                    R T+PL+LIHSDICDLKFVQTR G KYFITF+DDST+YCYVYLLKSKDEAI
Sbjct: 515 TRSSFQIVERNTEPLDLIHSDICDLKFVQTRGGNKYFITFVDDSTKYCYVYLLKSKDEAI 574

BLAST of CSPI01G21160 vs. NCBI nr
Match: CAN66637.1 (hypothetical protein VITISV_011340 [Vitis vinifera])

HSP 1 Score: 659.1 bits (1699), Expect = 3.4e-185
Identity = 359/685 (52.41%), Postives = 447/685 (65.26%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 35  VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 94

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE  G+KKF+VG+FLDYKMVD
Sbjct: 95  SDFLCRNYVMNGLADSLYNVYSDKKTAKELWESLDRKYKTEDAGAKKFVVGRFLDYKMVD 154

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 155 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 214

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQS--------------------NKKR 252
           L+++L IEE+NR++EK       ++KAN VE+   S                    +KK 
Sbjct: 215 LIIRLRIEEDNRRSEKKGAHTLNEAKANFVEHGQSSKAKTNNNKGKGSKLGPKGGISKKP 274

Query: 253 KFFGE--------------------------EVDEVSDGVANIDLCAVILECNMV-DNSK 312
           KF G+                           +D+++  V +IDL AV+ E N+V  N K
Sbjct: 275 KFQGKCFNCGKQGHKSVDCRLPKKNKPKEANVIDDITKNVYDIDLTAVVSEVNLVGSNPK 334

Query: 313 EWWVDTGATRHICANKDMFTSYVPVE----------------GQGKVILKMTSGKELTLN 372
           EWW+DTGATRH+C++K MF+++ P+E                GQGKVILKMTSGKELTL 
Sbjct: 335 EWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLT 394

Query: 373 NALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVH 432
           N L+VP IRKNLVSGSLL+ +GF+LVF SNK VLSK+ MY+GKGY+SDG++KLNV+T++ 
Sbjct: 395 NVLYVPEIRKNLVSGSLLNNHGFRLVFXSNKVVLSKSGMYVGKGYMSDGMWKLNVMTII- 454

Query: 433 KSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMC----- 492
           KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN+N IP F  ++NH CE C     
Sbjct: 455 KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLINLNHIPTFQINSNHKCETCVEAKL 514

Query: 493 ---------RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAI 549
                    R T+PL+LIHSDICDLKFVQTR G KYFITF+DDST+YCYVYLLKSKDEAI
Sbjct: 515 TRSSFQSVERNTEPLDLIHSDICDLKFVQTRGGNKYFITFVDDSTKYCYVYLLKSKDEAI 574

BLAST of CSPI01G21160 vs. NCBI nr
Match: RVX00859.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 658.7 bits (1698), Expect = 4.5e-185
Identity = 359/685 (52.41%), Postives = 447/685 (65.26%), Query Frame = 0

Query: 13  VAHGKKPEKFGGVDFKRWQQKMLFYLTTLNLAKFLTEDAPILPEGGSD------------ 72
           V+ G+KPEKF G++FKRWQQKMLFYLTTLNLA+FLTEDAP L E   D            
Sbjct: 9   VSPGEKPEKFSGLNFKRWQQKMLFYLTTLNLARFLTEDAPKLKEDEHDIQVISAIDAWKH 68

Query: 73  ---------------------KEKKL---------KKYKTEVVGSKKFIVGKFLDYKMVD 132
                                 +KK          +KYKTE  G+KKF+VG+FLDYKMVD
Sbjct: 69  SDFLCRNYVMNGLADSLYNVYSDKKTAKELWESLDRKYKTEDAGAKKFVVGRFLDYKMVD 128

Query: 133 FKTVINQVQEIQVILYDIYAENMTLSESFQ-----------------------------E 192
            KTV++QVQE+QVIL++I+AE M LSE+FQ                             +
Sbjct: 129 SKTVVSQVQELQVILHEIHAEGMMLSETFQVAAIIEKLPPAWKDFKNYLKHKRKEMSIED 188

Query: 193 LVVQLGIEENNRKAEKCTMDNTMDSKANIVENRSQS--------------------NKKR 252
           L+++L IEE+NR++EK       ++KAN VE+   S                    +KK 
Sbjct: 189 LIIRLRIEEDNRRSEKKGAHTLNEAKANFVEHGQSSKAKTNNNKGKGSKLGPKGGISKKP 248

Query: 253 KFFGE--------------------------EVDEVSDGVANIDLCAVILECNMV-DNSK 312
           KF G+                           +D+++  V +IDL AV+ E N+V  N K
Sbjct: 249 KFQGKCFNCGKQGHKSVDCRLPKKNKPKEANVIDDITKNVYDIDLTAVVSEVNLVGSNPK 308

Query: 313 EWWVDTGATRHICANKDMFTSYVPVE----------------GQGKVILKMTSGKELTLN 372
           EWW+DTGATRH+C++K MF+++ P+E                GQGKVILKMTSGKELTL 
Sbjct: 309 EWWIDTGATRHVCSDKKMFSTFEPIENGEKVFMGNSATSEIKGQGKVILKMTSGKELTLT 368

Query: 373 NALHVPNIRKNLVSGSLLSKNGFKLVFVSNKFVLSKNEMYIGKGYLSDGLFKLNVLTVVH 432
           N L+VP IRKNLVSGSLL+ +GF+LVF SNK VLSK+ MY+GKGY+SDG++KLNV+T++ 
Sbjct: 369 NVLYVPEIRKNLVSGSLLNNHGFRLVFESNKVVLSKSGMYVGKGYMSDGMWKLNVMTII- 428

Query: 433 KSIINKVSTLAYIVESF-VWHGRLGHVNFNSLQRLINMNLIPKFTFDTNHGCEMC----- 492
           KS +NK ST  Y++ES  +WHGRLGHVN+++L+RLIN+N IP F  ++NH CE C     
Sbjct: 429 KSNMNKASTSTYMLESSNLWHGRLGHVNYDTLRRLINLNHIPTFQINSNHKCETCVEAKL 488

Query: 493 ---------RITKPLELIHSDICDLKFVQTRCGKKYFITFIDDSTRYCYVYLLKSKDEAI 549
                    R T+PL+LIHSDICDLKFVQTR G KYFITF+DDST+YCYVYLLKSKDEAI
Sbjct: 489 TRSSFQSVERNTEPLDLIHSDICDLKFVQTRGGNKYFITFVDDSTKYCYVYLLKSKDEAI 548

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P109789.7e-5833.25Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Q9ZT941.5e-3729.13Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Q94HW24.7e-3629.97Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
P041464.0e-3527.04Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
Q124915.8e-1824.55Transposon Ty2-B Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20... [more]
Match NameE-valueIdentityDescription
A0A438E8364.7e-18855.09Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A0A438K0G85.7e-18652.55Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A0A438K1P89.8e-18652.55Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A5AEN51.7e-18552.41Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_011340 PE=4 SV=1[more]
A0A438IVT62.2e-18552.41Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
Match NameE-valueIdentityDescription
RVW43863.19.7e-18855.09Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
RVX14679.11.2e-18552.55Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
RVX15136.12.0e-18552.55Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
CAN66637.13.4e-18552.41hypothetical protein VITISV_011340 [Vitis vinifera][more]
RVX00859.14.5e-18552.41Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025724GAG-pre-integrase domainPFAMPF13976gag_pre-integrscoord: 305..356
e-value: 2.9E-12
score: 46.3
IPR001584Integrase, catalytic corePFAMPF00665rvecoord: 355..454
e-value: 3.1E-15
score: 56.3
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 353..517
score: 24.374893
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 350..526
e-value: 1.9E-39
score: 137.0
NoneNo IPR availablePANTHERPTHR47592:SF11SUBFAMILY NOT NAMEDcoord: 10..163
NoneNo IPR availablePANTHERPTHR47592:SF11SUBFAMILY NOT NAMEDcoord: 179..275
NoneNo IPR availablePANTHERPTHR47592PBF68 PROTEINcoord: 179..275
NoneNo IPR availablePANTHERPTHR47592PBF68 PROTEINcoord: 10..163
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 353..511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G21160.1CSPI01G21160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0003676 nucleic acid binding