CSPI01G20830 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G20830
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhospholipase A2-alpha
LocationChr1: 16419432 .. 16422709 (-)
RNA-Seq ExpressionCSPI01G20830
SyntenyCSPI01G20830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAAAAGCACTTCAACTATTTTCCTCTTGTTTTTCTTTAATCCCAAAACACTTTTCTCTTTACTCAAAAACCCCACAATTTCTTGTCAAACCAAAATTCATTCAGTCTTTCCAACAACCAAAAGAACAAGAAAATGCCTCAATTTCTTCTTCTTCTTCTTCTGTTGCCTTCCATTGTCTTTCTTCCTTCTCATGCTCTCAACATTGGTGTCCAAGCCCTTGATGCCTCCGTCACTATGGTTCTTCCTCATTTCATTCCCATCTTCTTTTTTCTTTAATTTGATCTTCTTCCTTCTTAATTGGTTTAATTTGTTCATATTGTAGAGTAAGGACTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGGTTAGTTTAAGAAGCTGTTGTTTGGAACAACTCTTTTTAATAACTCACTTGAAAAGTCAATTCAAACCAGTTCTGATTTATGTTTGATTTTGGTTCGAGCTTGTATTTGTATTTAATTGTTTGTGTGTGTTTTGTAGTGCCTCCACTGTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGTGGGTGCCCCGGGGAACACCCCTGTGATGGCTTGGATGCTTGTTGTATGAAGCATGATGCTTGTGTTGTTGCCAAGAACGGTTAGTACTACACATACTTCTCTATTCCCATCCAATTCTTTTTCTTTCCAACAATGCGTTAGGTCTAGAATCCTCAAGGCTCAAATCTTTGGTCTGAGAGATTGACAGTACATATTTGATAATCAATATTGATGTCAGTTTTAATTTGTTTTTCAGTACAACAAATGGAGTAGAAAGAGTGTTACTTTTTCTAAATACACGGAGTCAATAGATAAATAAGCCTTAGAGAAATTAAGATAATGGCACAATTAATATGGAATTGACAAAATTTTGGAGAAAACCAATACTTGCTATGCTAGAGCTAGTTTTTGAATGGTTAATATTTATGATTTGGTCCTATTGAGTGAAGTTTCCTTTTATTAAAATTAATGAATTTGTGAGGATTTTATAGTTTTTTTTATTTTGTTAAAATTGAATTTAGAATCCAATTTGACGAAAATTTTAACTTTCAAATTTGTTACGCTATACGAGTTTTTACTATCAAAATTGTTTTATCGTTCTTATTACATTGTAGCACCATTTGATGTGTTCATTCGTTTATTGCTATGGTTTAGTTCATATCTCATTAAAGTTGTTGGTATGATTTTAATGTGACGTGTTTGTTGAATAAACTTTGGTGTAATTAGTGTGATATGATAGGAAGTTGGACAAATGACGAATACGTCACATTCCAAAAGCGTTTTATAAATTAATTAGAAGTAGAGTTTGTATGGAATTATGTATTTGTTGTCATTTTAAAATAAATTACTAATTTAATTTTTAGTTTGTCAAAATAAACATATATGATATGTGTATTATCAATCTAAAAATTAAAACATATGATTTTGAGTACTCAATATTTTTCTTTTAAAATAATATTAGTAAAAATAAGACAATAATTTTAAAATAAACTAAAAAATATTTATCCATTCAAGATTTTAAATTTGGTCACCAATTTTCTAAATGAGTGTTGTGGGGTGTTAATACCTTCTCCACACAAAAATGACTCTCGAACTCAAACTCTATTTTTGGCCGACCATTTTTTTTTAAAATTGTTTACTTTATTTCGGTATCCAATCACACCCTAAAAAAGATTGGTGGCGATTCCTATATGTTTTAAAAAATAACTCTTTTAAAGACGTCAACCACCTTGTGTTGTTTCGGCAGATGGTGACACATACTTCTGATTGAATTTTGTCTGATCAATAATTTTGAAGAGTTTAGGGTATATGTGATGAGATGGCTAAATTTGTCTCTTTGCTTTGAGTGAACCAAAAAGGGTCACATGTAAAGGTGGCTATGAAGATCCTTTTTTAAAGCTTAATTTTATGGTTTATTTATTAAAAGAAAAAACATGAAATTAAATTCAAGACTTTTTAATTAATTTTCATTTTCAATATATATATTGTTTAATTAATGTGCAATAGACTTAGGGTTGGAGACCAAATTGAAATAGAGAATTGTTCTCATTGGAATGTGAGGAATTTCTACAAACATCAAAATTTGGATTTTGAACCTTTTCCAATAAAGAAAAATATTAATAATAAAATGGGCCCAAAAAGAAAAAAAAACTTTTGAAATATTTAACTTGGTTATTCAACTTTCCCTTTTATTTGATTTTAGTTTAAACTCTTCCAACTTGTTAACTTAAACTAATCCAAAGTTTTTTTTTGAAAAAAATAATACTCCAATTTAAAAATAAAAAAGAAAAAAAATAATTAAGTGCAATCGAAGTTGCTCGGAATCCTATACTTTCGAGTTTCATCTTCTTTGCATGCTAAAAATAAAACATTTGCTACGTCTAAGGTGAGTCAACCCTACTTACGTATACGACGCTTTCCAATTATTATTTCTTTCAGGGTTCAACACAACACATGTTGTGTGAGAGATTTGAACTATTGATCTATTAGTAAAAACCCGACGTTCTTAATACATGAAGAAATAGAAAATAATTGTGAGATTAGAAGTGAAACACAAAATTACTAGAAACCATTCAGAATTCACTTAAAATTTGAAGATGAGAGATAAAAAATTATGGTTCACTAGAATACGAAATCTCCTATGTAAAAACGTTACCGAATAATTCTCTTTGCTAATCTCAATTACAAGATCAACACCATTCCAAAGCGTTAAAGTCACTCACACAACTTAGTCCTCACTCCCAACTAGAATAATATCGACACAATTTGAAACCAAAACAAAAACTATAAACTCTTTTTGAAGTGTTTGGAGTACTCCACTGAAGTGGTGTATATGTGTTTTTGTTACATAATTTCAGATGACTATCTAAGCCAAGAATGCAGCCAAAGCTTCTTGAATTGCATGGAGAATTTCAAGAGATCAAGATCAAGTCCTTTCAAAGGAAACAAATGCCAAGTTGATGAAGTGATTGAAGTCATCTCCTTAGTCATGGAAGCTGCTCTTATTGCAGGCAGAGTTTTTCACAAACCCTAGCTAGCTAGCCACCTCTAACCTACCTTTTTATTTACAAATACCCAATTTCAATCATCAATCTCTTCCTTTTCCCCCTTCATTATTATGTATTGTTGTTTGGCATAAAACACATACACCAATTTCTCTCATATATATGAAATCACTATTCTATAACATGTTTTGAAGTGTTCGTTTTTTCGAGGATGGAGGATTGAATCCTAGGTTTTTAGGGAGG

mRNA sequence

AAACAAAAGCACTTCAACTATTTTCCTCTTGTTTTTCTTTAATCCCAAAACACTTTTCTCTTTACTCAAAAACCCCACAATTTCTTGTCAAACCAAAATTCATTCAGTCTTTCCAACAACCAAAAGAACAAGAAAATGCCTCAATTTCTTCTTCTTCTTCTTCTGTTGCCTTCCATTGTCTTTCTTCCTTCTCATGCTCTCAACATTGGTGTCCAAGCCCTTGATGCCTCCGTCACTATGAGTAAGGACTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGTGCCTCCACTGTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGTGGGTGCCCCGGGGAACACCCCTGTGATGGCTTGGATGCTTGTTGTATGAAGCATGATGCTTGTGTTGTTGCCAAGAACGATGACTATCTAAGCCAAGAATGCAGCCAAAGCTTCTTGAATTGCATGGAGAATTTCAAGAGATCAAGATCAAGTCCTTTCAAAGGAAACAAATGCCAAGTTGATGAAGTGATTGAAGTCATCTCCTTAGTCATGGAAGCTGCTCTTATTGCAGGCAGAGTTTTTCACAAACCCTAGCTAGCTAGCCACCTCTAACCTACCTTTTTATTTACAAATACCCAATTTCAATCATCAATCTCTTCCTTTTCCCCCTTCATTATTATGTATTGTTGTTTGGCATAAAACACATACACCAATTTCTCTCATATATATGAAATCACTATTCTATAACATGTTTTGAAGTGTTCGTTTTTTCGAGGATGGAGGATTGAATCCTAGGTTTTTAGGGAGG

Coding sequence (CDS)

ATGCCTCAATTTCTTCTTCTTCTTCTTCTGTTGCCTTCCATTGTCTTTCTTCCTTCTCATGCTCTCAACATTGGTGTCCAAGCCCTTGATGCCTCCGTCACTATGAGTAAGGACTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGTGCCTCCACTGTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGTGGGTGCCCCGGGGAACACCCCTGTGATGGCTTGGATGCTTGTTGTATGAAGCATGATGCTTGTGTTGTTGCCAAGAACGATGACTATCTAAGCCAAGAATGCAGCCAAAGCTTCTTGAATTGCATGGAGAATTTCAAGAGATCAAGATCAAGTCCTTTCAAAGGAAACAAATGCCAAGTTGATGAAGTGATTGAAGTCATCTCCTTAGTCATGGAAGCTGCTCTTATTGCAGGCAGAGTTTTTCACAAACCCTAG

Protein sequence

MPQFLLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIEVISLVMEAALIAGRVFHKP*
Homology
[Close]
BLAST of CSPI01G20830 vs. ExPASy Swiss-Prot
Match: Q8S8N6 (Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 8.5e-57
Identity = 96/129 (74.42%), Postives = 112/129 (86.82%), Query Frame = 0 

Query: 21  ALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACC 80
           ALN+GVQ +  S++++K+CSRKCESEFCSVPP LRYGKYCGLLYSGCPGE PCDGLD+CC
Sbjct: 20  ALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDSCC 79

Query: 81  MKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIEVISLVMEAAL 140
           MKHDACV +KN+DYLSQECSQ F+NCM NF + +   FKGNKC  DEVI+VIS+VMEAAL
Sbjct: 80  MKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDVISIVMEAAL 139

Query: 141 IAGRVFHKP 150
           IAG+V  KP
Sbjct: 140 IAGKVLKKP 148

[Close]
BLAST of CSPI01G20830 vs. ExPASy Swiss-Prot
Match: Q10E50 (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.2e-44
Identity = 86/141 (60.99%), Postives = 106/141 (75.18%), Query Frame = 0 

Query: 9   LLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 68
           LLL ++V  P+ ALNIG+Q+     + +  CSR CES+ C+ PPLLRYGKYCG+LYSGCP
Sbjct: 25  LLLFAVVAPPAAALNIGLQSAGDGASKAGLCSRTCESDHCTTPPLLRYGKYCGILYSGCP 84

Query: 69  GEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEV 128
           GE PCD LDACCM HD CV AKN DYLS  C++  L C+    R  SS F+GNKC +DEV
Sbjct: 85  GEQPCDELDACCMHHDNCVQAKN-DYLSTACNEELLECLARL-REGSSTFQGNKCMIDEV 144

Query: 129 IEVISLVMEAALIAGRVFHKP 150
           I+VISLV+EAA++AGR+ HKP
Sbjct: 145 IDVISLVIEAAVVAGRLLHKP 163

[Close]
BLAST of CSPI01G20830 vs. ExPASy Swiss-Prot
Match: Q9XG81 (Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-II PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 6.3e-36
Identity = 75/147 (51.02%), Postives = 98/147 (66.67%), Query Frame = 0 

Query: 2   PQFLLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCG 61
           P+  +LLLLL   +   S  LNIG   L ++    + CSR CES+FC++ PLLRYGKYCG
Sbjct: 9   PRCSVLLLLL---LVTASRGLNIG-DLLGSTPAKDQGCSRTCESQFCTIAPLLRYGKYCG 68

Query: 62  LLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGN 121
           +LYSGCPGE PCD LDACCM HD CV   NDDYL+  C+++ L+C++   R   + F GN
Sbjct: 69  ILYSGCPGERPCDALDACCMVHDHCVDTHNDDYLNTMCNENLLSCID---RVSGATFPGN 128

Query: 122 KCQVDEVIEVISLVMEAALIAGRVFHK 149
           KC V +   VI  V+E A+ AG++ HK
Sbjct: 129 KCNVGQTASVIRGVIETAVFAGKILHK 148

[Close]
BLAST of CSPI01G20830 vs. ExPASy Swiss-Prot
Match: Q8GV50 (Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.6e-16
Identity = 43/93 (46.24%), Postives = 56/93 (60.22%), Query Frame = 0 

Query: 21  ALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACC 80
           AL + V  L A V   + CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 81  MKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 114
           M HD CV  K   Y+  +C + F  C+   K+S
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

[Close]
BLAST of CSPI01G20830 vs. ExPASy Swiss-Prot
Match: Q9XG80 (Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-I PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.6e-15
Identity = 48/135 (35.56%), Postives = 71/135 (52.59%), Query Frame = 0 

Query: 11  LPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGE 70
           L ++VFL +  L+       A+      CSR C +  C     +RYGKYCG+ +SGC GE
Sbjct: 7   LLALVFLAAGVLS------SATSPPPPPCSRSCAALNCDSVG-IRYGKYCGVGWSGCDGE 66

Query: 71  HPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIE 130
            PCD LDACC  HD CV  K    +S +C + F NCM   K++    F   KC  +  + 
Sbjct: 67  EPCDDLDACCRDHDHCVDKKG--LMSVKCHEKFKNCMRKVKKAGKIGF-SRKCPYEMAMA 126

Query: 131 VISLVMEAALIAGRV 146
            ++  M+ A++  ++
Sbjct: 127 TMTSGMDMAIMLSQL 131

[Close]
BLAST of CSPI01G20830 vs. ExPASy TrEMBL
Match: A0A0A0LUV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G433610 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.3e-79
Identity = 148/149 (99.33%), Postives = 148/149 (99.33%), Query Frame = 0 

Query: 1   MPQFLLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYC 60
           MPQF LLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYC
Sbjct: 1   MPQF-LLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYC 60

Query: 61  GLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKG 120
           GLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKG
Sbjct: 61  GLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKG 120

Query: 121 NKCQVDEVIEVISLVMEAALIAGRVFHKP 150
           NKCQVDEVIEVISLVMEAALIAGRVFHKP
Sbjct: 121 NKCQVDEVIEVISLVMEAALIAGRVFHKP 148

[Close]
BLAST of CSPI01G20830 vs. ExPASy TrEMBL
Match: A0A5D3DYM2 (Phospholipase A2-alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001950 PE=4 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 6.5e-76
Identity = 142/151 (94.04%), Postives = 145/151 (96.03%), Query Frame = 0 

Query: 1   MPQF--LLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGK 60
           MPQF  LLLLLLLPSI FLPSHALNIGVQALDAS+TMSKDCSRKCESEFCSVPPLLRYGK
Sbjct: 1   MPQFLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGK 60

Query: 61  YCGLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPF 120
           YCGLLYSGCPGE PCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRS PF
Sbjct: 61  YCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPF 120

Query: 121 KGNKCQVDEVIEVISLVMEAALIAGRVFHKP 150
           KGNKCQVDEVI+VIS VMEAALIAG+VFHKP
Sbjct: 121 KGNKCQVDEVIDVISFVMEAALIAGKVFHKP 151

[Close]
BLAST of CSPI01G20830 vs. ExPASy TrEMBL
Match: A0A1S3BWG7 (phospholipase A2-alpha OS=Cucumis melo OX=3656 GN=LOC103494216 PE=4 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.5e-75
Identity = 142/156 (91.03%), Postives = 145/156 (92.95%), Query Frame = 0 

Query: 1   MPQF-------LLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPL 60
           MPQF       LLLLLLLPSI FLPSHALNIGVQALDAS+TMSKDCSRKCESEFCSVPPL
Sbjct: 1   MPQFLLLLLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPL 60

Query: 61  LRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120
           LRYGKYCGLLYSGCPGE PCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120

Query: 121 RSSPFKGNKCQVDEVIEVISLVMEAALIAGRVFHKP 150
           RS PFKGNKCQVDEVI+VIS VMEAALIAG+VFHKP
Sbjct: 121 RSRPFKGNKCQVDEVIDVISFVMEAALIAGKVFHKP 156

[Close]
BLAST of CSPI01G20830 vs. ExPASy TrEMBL
Match: A0A6J1F1D2 (phospholipase A2-alpha OS=Cucurbita moschata OX=3662 GN=LOC111438456 PE=4 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.2e-64
Identity = 118/146 (80.82%), Postives = 132/146 (90.41%), Query Frame = 0 

Query: 4   FLLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 63
           F   LLL  +IV  P+HALN+GVQALDAS+T+SKDCSRKCESEFCSVPPLLRYGKYCGLL
Sbjct: 7   FNSFLLLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLL 66

Query: 64  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 123
           YSGCPGE PCDGLDACCM HDACV AKN+DYLS+ECS+SFLNCME FKRS+S+PFKGNKC
Sbjct: 67  YSGCPGERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKC 126

Query: 124 QVDEVIEVISLVMEAALIAGRVFHKP 150
           ++ EV+EVIS VMEAAL+AGRV HKP
Sbjct: 127 EISEVVEVISFVMEAALLAGRVLHKP 152

[Close]
BLAST of CSPI01G20830 vs. ExPASy TrEMBL
Match: A0A6J1C0I6 (phospholipase A2-alpha OS=Momordica charantia OX=3673 GN=LOC111007165 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.0e-64
Identity = 121/146 (82.88%), Postives = 134/146 (91.78%), Query Frame = 0 

Query: 6   LLLLLLPSIVFL--PSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 65
           LLLLL  S++ L  P+ ALNIGVQA+DAS+++SKDCSRKCESEFCSVPP LRYGKYCGLL
Sbjct: 10  LLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLL 69

Query: 66  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 125
           YSGCPGE PCDGLDACCMKHD CV AKN+DYLSQECSQSFLNCMENFKRS+ + FKGNKC
Sbjct: 70  YSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKGNKC 129

Query: 126 QVDEVIEVISLVMEAALIAGRVFHKP 150
           Q+DEVI+VISLVMEAALIAG+VFHKP
Sbjct: 130 QIDEVIDVISLVMEAALIAGKVFHKP 155

[Close]
BLAST of CSPI01G20830 vs. NCBI nr
Match: XP_004150744.1 (phospholipase A2-alpha [Cucumis sativus] >KGN65513.1 hypothetical protein Csa_019839 [Cucumis sativus])

HSP 1 Score: 303.1 bits (775), Expect = 1.3e-78
Identity = 148/149 (99.33%), Postives = 148/149 (99.33%), Query Frame = 0 

Query: 1   MPQFLLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYC 60
           MPQF LLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYC
Sbjct: 1   MPQF-LLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYC 60

Query: 61  GLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKG 120
           GLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKG
Sbjct: 61  GLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKG 120

Query: 121 NKCQVDEVIEVISLVMEAALIAGRVFHKP 150
           NKCQVDEVIEVISLVMEAALIAGRVFHKP
Sbjct: 121 NKCQVDEVIEVISLVMEAALIAGRVFHKP 148

[Close]
BLAST of CSPI01G20830 vs. NCBI nr
Match: TYK28548.1 (phospholipase A2-alpha [Cucumis melo var. makuwa])

HSP 1 Score: 293.1 bits (749), Expect = 1.3e-75
Identity = 142/151 (94.04%), Postives = 145/151 (96.03%), Query Frame = 0 

Query: 1   MPQF--LLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGK 60
           MPQF  LLLLLLLPSI FLPSHALNIGVQALDAS+TMSKDCSRKCESEFCSVPPLLRYGK
Sbjct: 1   MPQFLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGK 60

Query: 61  YCGLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPF 120
           YCGLLYSGCPGE PCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRS PF
Sbjct: 61  YCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPF 120

Query: 121 KGNKCQVDEVIEVISLVMEAALIAGRVFHKP 150
           KGNKCQVDEVI+VIS VMEAALIAG+VFHKP
Sbjct: 121 KGNKCQVDEVIDVISFVMEAALIAGKVFHKP 151

[Close]
BLAST of CSPI01G20830 vs. NCBI nr
Match: XP_008453533.1 (PREDICTED: phospholipase A2-alpha [Cucumis melo])

HSP 1 Score: 291.2 bits (744), Expect = 5.1e-75
Identity = 142/156 (91.03%), Postives = 145/156 (92.95%), Query Frame = 0 

Query: 1   MPQF-------LLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPL 60
           MPQF       LLLLLLLPSI FLPSHALNIGVQALDAS+TMSKDCSRKCESEFCSVPPL
Sbjct: 1   MPQFLLLLLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPL 60

Query: 61  LRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120
           LRYGKYCGLLYSGCPGE PCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120

Query: 121 RSSPFKGNKCQVDEVIEVISLVMEAALIAGRVFHKP 150
           RS PFKGNKCQVDEVI+VIS VMEAALIAG+VFHKP
Sbjct: 121 RSRPFKGNKCQVDEVIDVISFVMEAALIAGKVFHKP 156

[Close]
BLAST of CSPI01G20830 vs. NCBI nr
Match: XP_038878664.1 (phospholipase A2-alpha [Benincasa hispida])

HSP 1 Score: 282.7 bits (722), Expect = 1.8e-72
Identity = 136/149 (91.28%), Postives = 142/149 (95.30%), Query Frame = 0 

Query: 1   MPQFLLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYC 60
           MPQFLLLLLL   I FLPS ALNIGVQALDAS+TMSKDCSRKCESEFCSVPPLLRYGKYC
Sbjct: 1   MPQFLLLLLLF--IAFLPSRALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYC 60

Query: 61  GLLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKG 120
           GLLYSGCPGE PCDGLDACCMKHDACVVAKNDDYLSQECSQSF+NCMENFKRS+S+PFKG
Sbjct: 61  GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFINCMENFKRSKSAPFKG 120

Query: 121 NKCQVDEVIEVISLVMEAALIAGRVFHKP 150
           NKC+VDEVI+VIS VMEAALIAGRVFHKP
Sbjct: 121 NKCEVDEVIDVISFVMEAALIAGRVFHKP 147

[Close]
BLAST of CSPI01G20830 vs. NCBI nr
Match: XP_022932135.1 (phospholipase A2-alpha [Cucurbita moschata] >KAG6587708.1 Phospholipase A2-alpha, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021676.1 Phospholipase A2-alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 255.8 bits (652), Expect = 2.4e-64
Identity = 118/146 (80.82%), Postives = 132/146 (90.41%), Query Frame = 0 

Query: 4   FLLLLLLLPSIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 63
           F   LLL  +IV  P+HALN+GVQALDAS+T+SKDCSRKCESEFCSVPPLLRYGKYCGLL
Sbjct: 7   FNSFLLLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLL 66

Query: 64  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 123
           YSGCPGE PCDGLDACCM HDACV AKN+DYLS+ECS+SFLNCME FKRS+S+PFKGNKC
Sbjct: 67  YSGCPGERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKC 126

Query: 124 QVDEVIEVISLVMEAALIAGRVFHKP 150
           ++ EV+EVIS VMEAAL+AGRV HKP
Sbjct: 127 EISEVVEVISFVMEAALLAGRVLHKP 152

[Close]
BLAST of CSPI01G20830 vs. TAIR 10
Match: AT2G06925.1 (Phospholipase A2 family protein )

HSP 1 Score: 221.1 bits (562), Expect = 6.1e-58
Identity = 96/129 (74.42%), Postives = 112/129 (86.82%), Query Frame = 0 

Query: 21  ALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACC 80
           ALN+GVQ +  S++++K+CSRKCESEFCSVPP LRYGKYCGLLYSGCPGE PCDGLD+CC
Sbjct: 20  ALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDSCC 79

Query: 81  MKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKCQVDEVIEVISLVMEAAL 140
           MKHDACV +KN+DYLSQECSQ F+NCM NF + +   FKGNKC  DEVI+VIS+VMEAAL
Sbjct: 80  MKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDVISIVMEAAL 139

Query: 141 IAGRVFHKP 150
           IAG+V  KP
Sbjct: 140 IAGKVLKKP 148

[Close]
BLAST of CSPI01G20830 vs. TAIR 10
Match: AT4G29470.1 (Phospholipase A2 family protein )

HSP 1 Score: 85.5 bits (210), Expect = 4.0e-17
Identity = 43/93 (46.24%), Postives = 56/93 (60.22%), Query Frame = 0 

Query: 21  ALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACC 80
           AL + V  L A V   + CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 81  MKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 114
           M HD CV  K   Y+  +C + F  C+   K+S
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

[Close]
BLAST of CSPI01G20830 vs. TAIR 10
Match: AT2G19690.2 (phospholipase A2-beta )

HSP 1 Score: 81.3 bits (199), Expect = 7.5e-16
Identity = 43/122 (35.25%), Postives = 66/122 (54.10%), Query Frame = 0 

Query: 33  VTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAKND 92
           V  S++C+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV     
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVELNGM 84

Query: 93  DYLSQECSQSFLNCMENFKRS-RSSPFK----GNKCQVDEVIEVISLVMEAALIAGRV-F 149
             +S  C + F  C+    ++ + S  K      KC    VI  ++  M+  ++  ++ +
Sbjct: 85  TNIS--CHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGILFSQLAY 143

[Close]
BLAST of CSPI01G20830 vs. TAIR 10
Match: AT4G29460.1 (Phospholipase A2 family protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.3e-15
Identity = 38/83 (45.78%), Postives = 50/83 (60.24%), Query Frame = 0 

Query: 31  ASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAK 90
           A V+  + CS  C ++ C+    +RYGKYCG+ Y GCPGE PCD LDACCM HD CV  K
Sbjct: 21  AVVSSQEKCSNTCIAQNCNSLG-IRYGKYCGIGYFGCPGEPPCDDLDACCMTHDNCVDLK 80

Query: 91  NDDYLSQECSQSFLNCMENFKRS 114
              Y++  C + F  C+    +S
Sbjct: 81  GMTYVN--CHKQFKRCVNKLSKS 100

[Close]
BLAST of CSPI01G20830 vs. TAIR 10
Match: AT2G19690.1 (phospholipase A2-beta )

HSP 1 Score: 80.1 bits (196), Expect = 1.7e-15
Identity = 42/114 (36.84%), Postives = 61/114 (53.51%), Query Frame = 0 

Query: 33  VTMSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGEHPCDGLDACCMKHDACVVAKND 92
           V  S++C+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV     
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVELNGM 84

Query: 93  DYLSQECSQSFLNCMENFKRS-RSSPFK----GNKCQVDEVIEVISLVMEAALI 142
             +S  C + F  C+    ++ + S  K      KC    VI  ++  M+  ++
Sbjct: 85  TNIS--CHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGIL 135

The following BLAST results are available for this feature:
BLAST of CSPI01G20830 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8S8N68.5e-5774.42Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1[more]
Q10E502.2e-4460.99Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III ... [more]
Q9XG816.3e-3651.02Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q8GV505.6e-1646.24Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1[more]
Q9XG801.6e-1535.56Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
BLAST of CSPI01G20830 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LUV26.3e-7999.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G433610 PE=4 SV=1[more]
A0A5D3DYM26.5e-7694.04Phospholipase A2-alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6... [more]
A0A1S3BWG72.5e-7591.03phospholipase A2-alpha OS=Cucumis melo OX=3656 GN=LOC103494216 PE=4 SV=1[more]
A0A6J1F1D21.2e-6480.82phospholipase A2-alpha OS=Cucurbita moschata OX=3662 GN=LOC111438456 PE=4 SV=1[more]
A0A6J1C0I62.0e-6482.88phospholipase A2-alpha OS=Momordica charantia OX=3673 GN=LOC111007165 PE=4 SV=1[more]
BLAST of CSPI01G20830 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004150744.11.3e-7899.33phospholipase A2-alpha [Cucumis sativus] >KGN65513.1 hypothetical protein Csa_01... [more]
TYK28548.11.3e-7594.04phospholipase A2-alpha [Cucumis melo var. makuwa][more]
XP_008453533.15.1e-7591.03PREDICTED: phospholipase A2-alpha [Cucumis melo][more]
XP_038878664.11.8e-7291.28phospholipase A2-alpha [Benincasa hispida][more]
XP_022932135.12.4e-6480.82phospholipase A2-alpha [Cucurbita moschata] >KAG6587708.1 Phospholipase A2-alpha... [more]
BLAST of CSPI01G20830 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G06925.16.1e-5874.42Phospholipase A2 family protein [more]
AT4G29470.14.0e-1746.24Phospholipase A2 family protein [more]
AT2G19690.27.5e-1635.25phospholipase A2-beta [more]
AT4G29460.11.3e-1545.78Phospholipase A2 family protein [more]
AT2G19690.11.7e-1536.84phospholipase A2-beta [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036444Phospholipase A2 domain superfamilyGENE3D1.20.90.10Phospholipase A2 domaincoord: 20..149
e-value: 1.7E-46
score: 159.0
IPR036444Phospholipase A2 domain superfamilySUPERFAMILY48619Phospholipase A2, PLA2coord: 54..148
IPR001211Phospholipase A2PANTHERPTHR11716PHOSPHOLIPASE A2 FAMILY MEMBERcoord: 7..146
NoneNo IPR availablePANTHERPTHR11716:SF87PHOSPHOLIPASE A2 HOMOLOG 2-RELATEDcoord: 7..146
NoneNo IPR availableCDDcd04706PLA2_plantcoord: 33..149
e-value: 5.95106E-58
score: 174.159
IPR033113Phospholipase A2, histidine active sitePROSITEPS00118PA2_HIScoord: 79..86

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G20830.1CSPI01G20830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050482 arachidonic acid secretion
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006644 phospholipid metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0047498 calcium-dependent phospholipase A2 activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005543 phospholipid binding
molecular_function GO:0004623 phospholipase A2 activity