CSPI01G20490 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G20490
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Chr1: 16170484 .. 16172193 (+)
RNA-Seq Expression	CSPI01G20490
Synteny	CSPI01G20490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTTCTACATGTTATTAAATATGGTTTCATTCTGCTTTGTGAGATCACCACGGTTTCGTTCTCCATTGTATAACACATCAGAAGCAACATAATGATTCTGTTAGCTTCTCTCCATCATCTTTTACCTTCCCTGATCTTGGCATTTTATTTGTCAACAGCCATAATCTCGTCCACATTGATCACGACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCATATCTACATCCACTCTATGACCAAAATGAGACAGTTGAGGATCGATCGAAGAGAGAGCAGACAAGCTCAATCGAACGCTTTGGTTTTCTCGAGTCAAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCAATCGGTTCGCCACCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGACCTCATGGTTCGATCCTTTGAAATCAGTAAGTTTCAAAACATTGGGTTGTGGGTTTCCTGGGTATAACTACATTAATGGTTACAAATGCAATCGGTTTAATCAAGCTGAGTACAAGTTGAGGTACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCCAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGTAGAGTTTTTCAATACAATGCTATTTCAACACAAATAAGTTGATAAATAAATTTGACTTTCTTAATTGTTTTAGTAACATATTTTACCGTGTTTTCTATAATTATTATTTTGTGATATCATATGATTTGTAATACACTATATGATTTAACAGAAAAGAATTGAAATTATAACATAGAAAAACTATATGAACTTTAATTTGTAATTACAACATTTGTCAAACTCACAATGGTATTCGATTGTTGTAACAGGCAAAATAAAAAAGTCGAACGTAACATTTGGGTGTGGTCATATGAACATCAAAACCAACAATGACGATGCCTACAATGGCGTATTTGGATTAGGAGCATACCCCCACATAACAATGGCCACTCAATTAGGCAACAAATTTTCCTATTGCATTGGCGATATCAACAATCCTCTCTACACTCACAATCATCTTGTCTTAGGACAAGGATCTTACATTGAAGGCGATTCCACTCCTCTTCAAATCCACTTTGGCCATTATTATGTCACTTTACAATCCATCAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAAATCTCATCGGACGGCAGCGGTGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGCTGGAACGAATCCCAACTCAGAGGAAATTCGAAGGGTTGTGTTTTAAAGGTGTCGTCAGTCGAGACCTTGTTGGGTTTCCGGCGGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACATGGTGGGGATCGGTTTTGTTTAGCTATTCTGCCAAGTAATTCTGAGTTGTTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTTGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGACGAGTAA

mRNA sequence

TGGTTCTACATGTTATTAAATATGGTTTCATTCTGCTTTGTGAGATCACCACGGTTTCGTTCTCCATTGTATAACACATCAGAAGCAACATAATGATTCTGTTAGCTTCTCTCCATCATCTTTTACCTTCCCTGATCTTGGCATTTTATTTGTCAACAGCCATAATCTCGTCCACATTGATCACGACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCATATCTACATCCACTCTATGACCAAAATGAGACAGTTGAGGATCGATCGAAGAGAGAGCAGACAAGCTCAATCGAACGCTTTGGTTTTCTCGAGTCAAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCAATCGGTTCGCCACCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGACCTCATGGTTCGATCCTTTGAAATCAGTAAGTTTCAAAACATTGGGTTGTGGGTTTCCTGGGTATAACTACATTAATGGTTACAAATGCAATCGGTTTAATCAAGCTGAGTACAAGTTGAGGTACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCCAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGCAAAATAAAAAAGTCGAACGTAACATTTGGGTGTGGTCATATGAACATCAAAACCAACAATGACGATGCCTACAATGGCGTATTTGGATTAGGAGCATACCCCCACATAACAATGGCCACTCAATTAGGCAACAAATTTTCCTATTGCATTGGCGATATCAACAATCCTCTCTACACTCACAATCATCTTGTCTTAGGACAAGGATCTTACATTGAAGGCGATTCCACTCCTCTTCAAATCCACTTTGGCCATTATTATGTCACTTTACAATCCATCAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAAATCTCATCGGACGGCAGCGGTGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGCTGGAACGAATCCCAACTCAGAGGAAATTCGAAGGGTTGTGTTTTAAAGGTGTCGTCAGTCGAGACCTTGTTGGGTTTCCGGCGGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACATGGTGGGGATCGGTTTTGTTTAGCTATTCTGCCAAGTAATTCTGAGTTGTTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTTGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGACGAGTAA

Coding sequence (CDS)

ATGATTCTGTTAGCTTCTCTCCATCATCTTTTACCTTCCCTGATCTTGGCATTTTATTTGTCAACAGCCATAATCTCGTCCACATTGATCACGACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCATATCTACATCCACTCTATGACCAAAATGAGACAGTTGAGGATCGATCGAAGAGAGAGCAGACAAGCTCAATCGAACGCTTTGGTTTTCTCGAGTCAAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCAATCGGTTCGCCACCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGACCTCATGGTTCGATCCTTTGAAATCAGTAAGTTTCAAAACATTGGGTTGTGGGTTTCCTGGGTATAACTACATTAATGGTTACAAATGCAATCGGTTTAATCAAGCTGAGTACAAGTTGAGGTACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCCAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGCAAAATAAAAAAGTCGAACGTAACATTTGGGTGTGGTCATATGAACATCAAAACCAACAATGACGATGCCTACAATGGCGTATTTGGATTAGGAGCATACCCCCACATAACAATGGCCACTCAATTAGGCAACAAATTTTCCTATTGCATTGGCGATATCAACAATCCTCTCTACACTCACAATCATCTTGTCTTAGGACAAGGATCTTACATTGAAGGCGATTCCACTCCTCTTCAAATCCACTTTGGCCATTATTATGTCACTTTACAATCCATCAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAAATCTCATCGGACGGCAGCGGTGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGCTGGAACGAATCCCAACTCAGAGGAAATTCGAAGGGTTGTGTTTTAAAGGTGTCGTCAGTCGAGACCTTGTTGGGTTTCCGGCGGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACATGGTGGGGATCGGTTTTGTTTAGCTATTCTGCCAAGTAATTCTGAGTTGTTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTTGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGACGAGTAA

Protein sequence

MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVEDRSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE*

Homology

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BLAST of CSPI01G20490 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 9.2e-46
Identity = 140/461 (30.37%), Postives = 232/461 (50.33%), Query Frame = 0


Query: 14  LILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVEDRSKREQTSSIERF 73
           ++L F+L  ++  S+  +  P   + +LIHR+S L P+Y+   TV DR       S+ R 
Sbjct: 5   ILLCFFLFFSVTLSS--SGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS 64

Query: 74  GFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLP 133
                ++ +      + +S LI       F ++++IG+PP+    + DTGS L WVQC P
Sbjct: 65  RRFNHQLSQ-----TDLQSGLI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKP 124

Query: 134 CINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYK--CNRFNQAEYKLRYLGGDS--S 193
           C  C++++   FD  KS ++K+  C       ++  +  C+  N    K RY  GD   S
Sbjct: 125 CQQCYKENGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNI-CKYRYSYGDQSFS 184

Query: 194 QGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPHITMATQLG 253
           +G +A E++  ++     +      FGCG+ N  T  D+  +G+ GLG   H+++ +QLG
Sbjct: 185 KGDVATETVSIDSASGSPVSFPGTVFGCGYNNGGT-FDETGSGIIGLGG-GHLSLISQLG 244

Query: 254 N----KFSYCIGDINNPLYTHNHLVLGQG-----SYIEGD----STPL--QIHFGHYYVT 313
           +    KFSYC+   +    T+   V+  G     S +  D    STPL  +    +YY+T
Sbjct: 245 SSISKKFSYCLS--HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLT 304

Query: 314 LQSISVGSKTL-----KIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLM 373
           L++ISVG K +       +PN   I S+ SG ++IDSG T T L  G F+     + + +
Sbjct: 305 LEAISVGKKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESV 364

Query: 374 KGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCL 433
            G  +R+   +     CFK   +   +G P +T HF  GAD+ L   + F +   D  CL
Sbjct: 365 TG-AKRVSDPQGLLSHCFKSGSAE--IGLPEITVHFT-GADVRLSPINAFVKLSEDMVCL 424

Query: 434 AILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 451
           +++P+      +++ G  AQ ++ VG+DLE   V F+ +DC
Sbjct: 425 SMVPTT----EVAIYGNFAQMDFLVGYDLETRTVSFQHMDC 443

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BLAST of CSPI01G20490 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 6.0e-45
Identity = 141/457 (30.85%), Postives = 212/457 (46.39%), Query Frame = 0


Query: 11  LPSLILAFYLSTAIISSTLITTKPSR----LATKLIHRNSYLHPLYDQNETVEDRSKREQ 70
           + SL  +  LS  ++SS  ++   ++        LIHR+S   P Y+  ET   R +   
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAI 60

Query: 71  TSSIER-FGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSS 130
             S+ R F F E          N  +  +   +    +L+N+SIG+PP   + + DTGS 
Sbjct: 61  HRSVNRVFHFTEK--------DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSD 120

Query: 131 LLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYI-NGYKCN-RFNQAEYKLRY 190
           LLW QC PC +C+ Q    FDP  S ++K + C       + N   C+   N   Y L Y
Sbjct: 121 LLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSY 180

Query: 191 LGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPHIT 250
                ++G +A ++L   + D   ++  N+  GCGH N  T N    +G+ GLG  P ++
Sbjct: 181 GDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKG-SGIVGLGGGP-VS 240

Query: 251 MATQLGN----KFSYCIGDINNPLYTHNHLVLGQGSYIEGD---STPLQIHFGH---YYV 310
           +  QLG+    KFSYC+  + +     + +  G  + + G    STPL         YY+
Sbjct: 241 LIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYL 300

Query: 311 TLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLL 370
           TL+SISVGSK ++   +    S    G ++IDSG T T L        Y E+ D +   +
Sbjct: 301 TLKSISVGSKQIQYSGSD---SESSEGNIIIDSGTTLTLLPTE----FYSELEDAVASSI 360

Query: 371 ERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILP 430
           +    Q    GL      + DL   P +T HF  GAD+ L+S + F Q   D  C A   
Sbjct: 361 DAEKKQDPQSGLSLCYSATGDL-KVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAFRG 420

Query: 431 SNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 451
           S S     S+ G +AQ N+ VG+D     V F+  DC
Sbjct: 421 SPS----FSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 434

[Close]

BLAST of CSPI01G20490 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.3e-43
Identity = 121/356 (33.99%), Postives = 172/356 (48.31%), Query Frame = 0


Query: 103 FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPG 162
           +L+NLSIG+P      ++DTGS L+W QC PC  CF QST  F+P  S SF TL C    
Sbjct: 95  YLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQL 154

Query: 163 YNYINGYKCNRFNQAEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNI 222
              ++   C+  N  +Y   Y  G  +QG +  E+L F     G +   N+TFGCG  N 
Sbjct: 155 CQALSSPTCSN-NFCQYTYGYGDGSETQGSMGTETLTF-----GSVSIPNITFGCGENNQ 214

Query: 223 KTNNDDAYNGVFGLGAYPHITMATQLG-NKFSYCIGDINNPLYTHNHLVLGQ--GSYIEG 282
                +   G+ G+G  P +++ +QL   KFSYC+  I +   T ++L+LG    S   G
Sbjct: 215 GFGQGNG-AGLVGMGRGP-LSLPSQLDVTKFSYCMTPIGSS--TPSNLLLGSLANSVTAG 274

Query: 283 DSTPLQIHFGH----YYVTLQSISVGSKTLKIDPNAFKI-SSDGSGGVLIDSGMTYTKLA 342
                 I        YY+TL  +SVGS  L IDP+AF + S++G+GG++IDSG T T   
Sbjct: 275 SPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFV 334

Query: 343 NGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLE 402
           N  ++ +  E +  +   L  +        LCF+       +  P    HF GG DL L 
Sbjct: 335 NNAYQSVRQEFISQIN--LPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELP 394

Query: 403 SGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 451
           S + F        CLA+    S    +S+ G + QQN  V +D     V F    C
Sbjct: 395 SENYFISPSNGLICLAM---GSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of CSPI01G20490 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.4e-40
Identity = 128/446 (28.70%), Postives = 206/446 (46.19%), Query Frame = 0


Query: 11  LPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVEDRSKREQTSSI 70
           L S++L   + +AI++ T  T++ + L          L    +Q ++ ++ +K E     
Sbjct: 5   LYSVVLGLAIVSAIVAPTSSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKRA 64

Query: 71  ERFGFLESKIKELKSVGNEARSSLIPFNRGSG-FLVNLSIGSPPVTQLVVVDTGSSLLWV 130
            + G  E +++ + ++   +     P   G G +L+N++IG+P  +   ++DTGS L+W 
Sbjct: 65  IKRG--ERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWT 124

Query: 131 QCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYKLRYLGGDSS 190
           QC PC  CF Q T  F+P  S SF TL C       +    CN  N+ +Y   Y  G ++
Sbjct: 125 QCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNN-NECQYTYGYGDGSTT 184

Query: 191 QGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPHITMATQLG 250
           QG +A E+  FET         N+ FGCG  N      +   G+ G+G  P +++ +QLG
Sbjct: 185 QGYMATETFTFET-----SSVPNIAFGCGEDNQGFGQGNG-AGLIGMGWGP-LSLPSQLG 244

Query: 251 -NKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFG----HYYVTLQSISVGSKT 310
             +FSYC+    +   +   L        EG  +   IH      +YY+TLQ I+VG   
Sbjct: 245 VGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDN 304

Query: 311 LKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEG 370
           L I  + F++  DG+GG++IDSG T T L    +  +     D +   L  +        
Sbjct: 305 LGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQIN--LPTVDESSSGLS 364

Query: 371 LCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVI 430
            CF+       V  P ++  F GG   + E   L     G   CLA+   +S  L +S+ 
Sbjct: 365 TCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILISPAEG-VICLAM--GSSSQLGISIF 424

Query: 431 GILAQQNYNVGFDLEQMKVFFRRIDC 451
           G + QQ   V +DL+ + V F    C
Sbjct: 425 GNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of CSPI01G20490 vs. ExPASy Swiss-Prot
Match: Q9SV77 (Aspartyl protease UND OS=Arabidopsis thaliana OX=3702 GN=UND PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.2e-38
Identity = 112/360 (31.11%), Postives = 175/360 (48.61%), Query Frame = 0


Query: 99  RGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQST-SWFDPLKSVSFKTLG 158
           RG  F+  +  GSP   Q + +DTGSSL W QC PC +C+ Q     + P  S++++   
Sbjct: 54  RGLAFMAEIHFGSPQKKQFLHMDTGSSLTWTQCFPCSDCYAQKIYPKYRPAASITYRDAM 113

Query: 159 CGFPGYNYINGYKCNRFNQ-AEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFG 218
           C          +  +   +   Y+  YL   + +G LA+E +  +T D G  +   V FG
Sbjct: 114 CEDSHPKSNPHFAFDPLTRICTYQQHYLDETNIKGTLAQEMITVDTHDGGFKRVHGVYFG 173

Query: 219 CGHMNIKTNNDDAY---NGVFGLGAYPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQ 278
           C      T +D +Y    G+ GLG   + ++  + G+KFS+C+G+I+ P  +HN L+LG 
Sbjct: 174 C-----NTLSDGSYFTGTGILGLGVGKY-SIIGEFGSKFSFCLGEISEPKASHN-LILGD 233

Query: 279 GSYIEGDSTPLQIHFGHYYVTLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKL 338
           G+ ++G  T + I  GH    L+SI VG           +I+ D    V +D+G T + L
Sbjct: 234 GANVQGHPTVINITEGHTIFQLESIIVGE----------EITLDDPVQVFVDTGSTLSHL 293

Query: 339 ANGGFELLYDEIVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVL 398
           +      LY + VD    L+   P   +   LC+K      L     V F F  GA+L +
Sbjct: 294 STN----LYYKFVDAFDDLIGSRPLSYE-PTLCYKADTIERLEKMD-VGFKFDVGAELSV 353

Query: 399 ESGSLFRQHGGDRF-CLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQL 453
              ++F Q G     CLAI  +N E  +  +IG++A Q YNVG+DL     +  + DC +
Sbjct: 354 NIHNIFIQQGPPEIRCLAI-QNNKESFSHVIIGVIAMQGYNVGYDLSAKTAYINKQDCDM 389

[Close]

BLAST of CSPI01G20490 vs. ExPASy TrEMBL
Match: A0A0A0LUP5 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G424350 PE=3 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 6.9e-262
Identity = 452/455 (99.34%), Postives = 453/455 (99.56%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           MILLASLHHLLPSL LAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED
Sbjct: 2   MILLASLHHLLPSLTLAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 61

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKREQTSSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV
Sbjct: 62  RSKREQTSSIERFDFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 121

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK
Sbjct: 122 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 181

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKKSN+TFGCGHMNIKTNNDDAYNGVFGLGAYP
Sbjct: 182 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNITFGCGHMNIKTNNDDAYNGVFGLGAYP 241

Query: 241 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 300
           HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV
Sbjct: 242 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 301

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 302 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 361

Query: 361 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 362 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 421

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 422 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456

[Close]

BLAST of CSPI01G20490 vs. ExPASy TrEMBL
Match: A0A5D3DZ20 (Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.0e-253
Identity = 440/455 (96.70%), Postives = 443/455 (97.36%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           MILLASLHHLLPSL LAFYLSTAII ST I TKPSRLATKLIHRNSYLHPLYD NETVED
Sbjct: 391 MILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVED 450

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKREQ SSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV
Sbjct: 451 RSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 510

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCINCFQQSTSWFDPLKS SFKTLGCGFPGYNYINGYKCN  NQAEYK
Sbjct: 511 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYK 570

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAYP
Sbjct: 571 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 630

Query: 241 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 300
           HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQ+ISV
Sbjct: 631 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISV 690

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 691 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 750

Query: 361 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 751 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 810

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 811 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

[Close]

BLAST of CSPI01G20490 vs. ExPASy TrEMBL
Match: A0A5A7UU11 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005510 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.0e-253
Identity = 440/455 (96.70%), Postives = 444/455 (97.58%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           MILLASLHHLLPSL LAFYLSTAII ST ITTKPSRLATKLIHRNSYLHPLYD NETVED
Sbjct: 391 MILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVED 450

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKREQ SSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV
Sbjct: 451 RSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 510

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCINCFQQSTSWFDPLKS SFKTLGCGFPGYNYINGYKCN  NQAEYK
Sbjct: 511 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYK 570

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAYP
Sbjct: 571 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 630

Query: 241 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 300
           +ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQ+ISV
Sbjct: 631 YITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISV 690

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 691 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 750

Query: 361 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 751 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 810

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 811 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

[Close]

BLAST of CSPI01G20490 vs. ExPASy TrEMBL
Match: A0A1S3BY28 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103494349 PE=3 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.3e-249
Identity = 438/471 (92.99%), Postives = 443/471 (94.06%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           MILLASLHHLLPSL LAFYLSTAII ST ITTKPSRLATKLIHRNSYLHPLYD NETVED
Sbjct: 15  MILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVED 74

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKREQ SSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV
Sbjct: 75  RSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 134

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCINCFQQSTSWFDPLKS SFKTLGCGFPGYNYINGYKCN  NQAEYK
Sbjct: 135 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYK 194

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKI----------------KKSNVTFGCGHMNIKT 240
           LRYLGGDSSQGILAKESLLFETLDEG +                KK+N+TFGCGHMN KT
Sbjct: 195 LRYLGGDSSQGILAKESLLFETLDEGGVFQYNALFQHKQVNKQNKKTNLTFGCGHMNFKT 254

Query: 241 NNDDAYNGVFGLGAYPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPL 300
           NNDD YNGVFGLGAYP+ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPL
Sbjct: 255 NNDDTYNGVFGLGAYPYITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPL 314

Query: 301 QIHFGHYYVTLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE 360
           QIHFGHYYVTLQ+ISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE
Sbjct: 315 QIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE 374

Query: 361 IVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGG 420
           IVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGG
Sbjct: 375 IVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGG 434

Query: 421 DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 435 DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 484

[Close]

BLAST of CSPI01G20490 vs. ExPASy TrEMBL
Match: A0A6J1C0L1 (probable aspartic protease At2g35615 OS=Momordica charantia OX=3673 GN=LOC111006977 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.6e-224
Identity = 380/454 (83.70%), Postives = 414/454 (91.19%), Query Frame = 0


Query: 3   LLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVEDRS 62
           LL SLHHLLP L  AFYLSTA+ISST +TTKPSRL TKLIHRNSYLHPLYD NETVEDRS
Sbjct: 4   LLLSLHHLLPFLTSAFYLSTAVISSTDVTTKPSRLVTKLIHRNSYLHPLYDPNETVEDRS 63

Query: 63  KREQTSSIERFGFLESKIKELKSV-GNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVD 122
           KRE+TSS ERF +LESKIKEL SV GNEAR++LIPFN+GSGFLVN SIG PPVTQL VVD
Sbjct: 64  KREETSSTERFAYLESKIKELNSVGGNEARANLIPFNQGSGFLVNFSIGQPPVTQLAVVD 123

Query: 123 TGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYKL 182
           TGSSLLWVQCLPC+NCF+QS SWFDPLKS SFK L C FPG+NY+ GYKCN F+QAEYKL
Sbjct: 124 TGSSLLWVQCLPCVNCFRQSGSWFDPLKSWSFKILDCDFPGHNYVRGYKCNDFHQAEYKL 183

Query: 183 RYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPH 242
           RYLGGD+S+GILAKESLLFET DEGKI+K+N+TFGCGHMN KTN DD YNGVFGLG YPH
Sbjct: 184 RYLGGDTSEGILAKESLLFETPDEGKIRKANLTFGCGHMNTKTNRDDTYNGVFGLGGYPH 243

Query: 243 ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISVG 302
           ITMATQLGNKFSYCIGDI +PLY HNHL LG G++IEGDSTPLQI FGHYYV+L+ ISVG
Sbjct: 244 ITMATQLGNKFSYCIGDITDPLYAHNHLFLGHGAFIEGDSTPLQIIFGHYYVSLEGISVG 303

Query: 303 SKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRK 362
           SK LKIDPNAF+++SDG GGVLIDSGMTYTKL NGGFELL+DEI DLMKG+LERIPT+RK
Sbjct: 304 SKRLKIDPNAFQMTSDGRGGVLIDSGMTYTKLTNGGFELLFDEIADLMKGVLERIPTRRK 363

Query: 363 FEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNL 422
           FEGLC+KGVV RDLVG P VTFHFAGGADLVLESGSLFRQHGGDRFCLA+LPSNSE++NL
Sbjct: 364 FEGLCYKGVVGRDLVGLPPVTFHFAGGADLVLESGSLFRQHGGDRFCLAVLPSNSEMMNL 423

Query: 423 SVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           SVIG+LAQQNYNVGFDLEQMKVFFRRIDCQLL +
Sbjct: 424 SVIGVLAQQNYNVGFDLEQMKVFFRRIDCQLLGD 457

[Close]

BLAST of CSPI01G20490 vs. NCBI nr
Match: XP_031742409.1 (aspartic proteinase CDR1 [Cucumis sativus] >KGN65478.1 hypothetical protein Csa_020065 [Cucumis sativus])

HSP 1 Score: 912.5 bits (2357), Expect = 1.4e-261
Identity = 452/455 (99.34%), Postives = 453/455 (99.56%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           MILLASLHHLLPSL LAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED
Sbjct: 2   MILLASLHHLLPSLTLAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 61

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKREQTSSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV
Sbjct: 62  RSKREQTSSIERFDFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 121

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK
Sbjct: 122 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 181

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKKSN+TFGCGHMNIKTNNDDAYNGVFGLGAYP
Sbjct: 182 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNITFGCGHMNIKTNNDDAYNGVFGLGAYP 241

Query: 241 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 300
           HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV
Sbjct: 242 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 301

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 302 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 361

Query: 361 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 362 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 421

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 422 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456

[Close]

BLAST of CSPI01G20490 vs. NCBI nr
Match: KAA0058227.1 (aspartic proteinase CDR1-like [Cucumis melo var. makuwa])

HSP 1 Score: 884.4 bits (2284), Expect = 4.2e-253
Identity = 440/455 (96.70%), Postives = 444/455 (97.58%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           MILLASLHHLLPSL LAFYLSTAII ST ITTKPSRLATKLIHRNSYLHPLYD NETVED
Sbjct: 391 MILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVED 450

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKREQ SSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV
Sbjct: 451 RSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 510

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCINCFQQSTSWFDPLKS SFKTLGCGFPGYNYINGYKCN  NQAEYK
Sbjct: 511 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYK 570

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAYP
Sbjct: 571 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 630

Query: 241 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 300
           +ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQ+ISV
Sbjct: 631 YITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISV 690

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 691 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 750

Query: 361 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 751 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 810

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 811 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

[Close]

BLAST of CSPI01G20490 vs. NCBI nr
Match: TYK28585.1 (Peptidase A1 [Cucumis melo var. makuwa])

HSP 1 Score: 884.4 bits (2284), Expect = 4.2e-253
Identity = 440/455 (96.70%), Postives = 443/455 (97.36%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           MILLASLHHLLPSL LAFYLSTAII ST I TKPSRLATKLIHRNSYLHPLYD NETVED
Sbjct: 391 MILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVED 450

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKREQ SSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV
Sbjct: 451 RSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 510

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCINCFQQSTSWFDPLKS SFKTLGCGFPGYNYINGYKCN  NQAEYK
Sbjct: 511 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYK 570

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAYP
Sbjct: 571 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 630

Query: 241 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 300
           HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQ+ISV
Sbjct: 631 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISV 690

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 691 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 750

Query: 361 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 751 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 810

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 811 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

[Close]

BLAST of CSPI01G20490 vs. NCBI nr
Match: XP_008453701.1 (PREDICTED: aspartic proteinase CDR1-like [Cucumis melo])

HSP 1 Score: 870.9 bits (2249), Expect = 4.8e-249
Identity = 438/471 (92.99%), Postives = 443/471 (94.06%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           MILLASLHHLLPSL LAFYLSTAII ST ITTKPSRLATKLIHRNSYLHPLYD NETVED
Sbjct: 15  MILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVED 74

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKREQ SSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV
Sbjct: 75  RSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 134

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCINCFQQSTSWFDPLKS SFKTLGCGFPGYNYINGYKCN  NQAEYK
Sbjct: 135 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEYK 194

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKI----------------KKSNVTFGCGHMNIKT 240
           LRYLGGDSSQGILAKESLLFETLDEG +                KK+N+TFGCGHMN KT
Sbjct: 195 LRYLGGDSSQGILAKESLLFETLDEGGVFQYNALFQHKQVNKQNKKTNLTFGCGHMNFKT 254

Query: 241 NNDDAYNGVFGLGAYPHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPL 300
           NNDD YNGVFGLGAYP+ITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPL
Sbjct: 255 NNDDTYNGVFGLGAYPYITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPL 314

Query: 301 QIHFGHYYVTLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE 360
           QIHFGHYYVTLQ+ISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE
Sbjct: 315 QIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE 374

Query: 361 IVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGG 420
           IVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGG
Sbjct: 375 IVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGG 434

Query: 421 DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 435 DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 484

[Close]

BLAST of CSPI01G20490 vs. NCBI nr
Match: XP_038878960.1 (aspartic proteinase CDR1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 834.7 bits (2155), Expect = 3.8e-238
Identity = 406/455 (89.23%), Postives = 430/455 (94.51%), Query Frame = 0


Query: 1   MILLASLHHLLPSLILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVED 60
           +ILL S +HLLPSL LAFYLSTAIISS   TTKPSRLATKLIHRNSYLHPLYD  ET+ED
Sbjct: 2   VILLVSQYHLLPSLTLAFYLSTAIISSMAFTTKPSRLATKLIHRNSYLHPLYDPTETIED 61

Query: 61  RSKREQTSSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVV 120
           RSKRE+TSSIERF +LESKIKELKSVGNEARSSLIPFN+GSGFLVNLSIGSPPVTQLVV 
Sbjct: 62  RSKREETSSIERFAYLESKIKELKSVGNEARSSLIPFNQGSGFLVNLSIGSPPVTQLVVA 121

Query: 121 DTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYK 180
           DTGSSLLWVQCLPCI+CF+QS SWFDPLKS SFK LGCGF GYNYI+GY+CN FNQAEYK
Sbjct: 122 DTGSSLLWVQCLPCIDCFRQSNSWFDPLKSTSFKILGCGFAGYNYISGYRCNGFNQAEYK 181

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYP 240
           LRYLGGD+SQG+LAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAYP
Sbjct: 182 LRYLGGDTSQGVLAKESLLFETLDEGKIKKTNLTFGCGHMNSKTNNDDTYNGVFGLGAYP 241

Query: 241 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSISV 300
           +ITMATQLGNKFSYCIGDINNP+YTHNHLVLG+GSYIEGDSTPLQIHFGHYYVTLQ ISV
Sbjct: 242 YITMATQLGNKFSYCIGDINNPVYTHNHLVLGEGSYIEGDSTPLQIHFGHYYVTLQGISV 301

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSK LKIDP AF+++ DG GGVLIDSGMTYTKLANGGFELLYDEI+DLM GLLERIPT+R
Sbjct: 302 GSKRLKIDPKAFQMTWDGRGGVLIDSGMTYTKLANGGFELLYDEILDLMTGLLERIPTER 361

Query: 361 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEGLCFKGVVSRDL+GFP VTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSE+LN
Sbjct: 362 KFEGLCFKGVVSRDLIGFPTVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEMLN 421

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
           LSVIGILAQQNYNV FDLEQMKVFFRRIDCQLLDE
Sbjct: 422 LSVIGILAQQNYNVAFDLEQMKVFFRRIDCQLLDE 456

[Close]

BLAST of CSPI01G20490 vs. TAIR 10
Match: AT4G30030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 315.1 bits (806), Expect = 9.4e-86
Identity = 167/396 (42.17%), Postives = 239/396 (60.35%), Query Frame = 0


Query: 61  RSKREQTSSIERFGFLESKIKELKSVGN-EARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           R+K +++S I + G+L SK      + N    S + P    + FL N+SIG+PPV QL++
Sbjct: 36  RTKTQESSKI-KIGYLHSKSTPASRLDNLWTVSHVTPIPNPAAFLANISIGNPPVPQLLL 95

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEY 180
           +DTGS L W+ CLPC  C+ Q+  +F P +S +++   C    +     ++  +    +Y
Sbjct: 96  IDTGSDLTWIHCLPC-KCYPQTIPFFHPSRSSTYRNASCVSAPHAMPQIFRDEKTGNCQY 155

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAY 240
            LRY    +++GILA+E L FET D+G I K N+ FGCG  N   +    Y+GV GLG  
Sbjct: 156 HLRYRDFSNTRGILAEEKLTFETSDDGLISKQNIVFGCGQDN---SGFTKYSGVLGLGPG 215

Query: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSIS 300
               +    G+KFSYC G + NP Y HN L+LG G+ IEGD TPLQI    YY+ LQ+IS
Sbjct: 216 TFSIVTRNFGSKFSYCFGSLTNPTYPHNILILGNGAKIEGDPTPLQIFQDRYYLDLQAIS 275

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
            G K L I+P  F+      GG +ID+G + T LA   +E L +EI  L+  +L R+   
Sbjct: 276 FGEKLLDIEPGTFQ-RYRSQGGTVIDTGCSPTILAREAYETLSEEIDFLLGEVLRRVKDW 335

Query: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLF-RQHGGDRFCLAILPSNSEL 420
            ++   C++G +  DL GFP VTFHFAGGA+L L+  SLF     GD FCLA+  +  + 
Sbjct: 336 DQYTTPCYEGNLKLDLYGFPVVTFHFAGGAELALDVESLFVSSESGDSFCLAMTMNTFD- 395

Query: 421 LNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLD 455
            ++SVIG +AQQNYNVG++L  MKV+F+R DC+++D
Sbjct: 396 -DMSVIGAMAQQNYNVGYNLRTMKVYFQRTDCEIID 423

[Close]

BLAST of CSPI01G20490 vs. TAIR 10
Match: AT2G23945.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-78
Identity = 179/457 (39.17%), Postives = 262/457 (57.33%), Query Frame = 0


Query: 13  SLILAFYLSTAIISSTLITTKPSRLATKLIHRNSY--LHPLYDQNETVEDRSKREQTSSI 72
           SL+L   +S  +++ ++   KP+R+A KLIHR S   L+P      T ED  K     S 
Sbjct: 8   SLLLFITVSYFVVTESI---KPNRMAMKLIHRESVARLNPNARVPITPEDHIKHLTDISS 67

Query: 73  ERFGFLESKI-KELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWV 132
            RF +L++ I KEL S  +  +  +    + S FLVN S+G PPV QL ++DTGSSLLW+
Sbjct: 68  ARFKYLQNSIDKELGS--SNFQVDVEQAIKTSLFLVNFSVGQPPVPQLTIMDTGSSLLWI 127

Query: 133 QCLPCINCFQQST--SWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYKLRYLGGD 192
           QC PC +C         F+P  S +F    C      Y     C   N+  Y+  Y+ G 
Sbjct: 128 QCQPCKHCSSDHMIHPVFNPALSSTFVECSCDDRFCRYAPNGHCGSSNKCVYEQVYISGT 187

Query: 193 SSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPHITMATQ 252
            S+G+LAKE L F T +   +    + FGCG+ N     +  + G+ GLGA P  ++A Q
Sbjct: 188 GSKGVLAKERLTFTTPNGNTVVTQPIAFGCGYEN-GEQLESHFTGILGLGAKP-TSLAVQ 247

Query: 253 LGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGH--YYVTLQSISVGSKTL 312
           LG+KFSYCIGD+ N  Y +N LVLG+ + I GD TP++    +  YY+ L+ ISVG   L
Sbjct: 248 LGSKFSYCIGDLANKNYGYNQLVLGEDADILGDPTPIEFETENSIYYMNLEGISVGDTQL 307

Query: 313 KIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGL 372
            I+P  FK     + GV++DSG  YT LA+  +  LY+EI  ++   LER   +   + L
Sbjct: 308 NIEPVVFKRRGPRT-GVILDSGTLYTWLADIAYRELYNEIKSILDPKLERFWFR---DFL 367

Query: 373 CFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLF----RQHGGDRFCLAILPS---NSEL 432
           C+ G VS +L+GFP VTFHFAGGA+L +E+ S+F      +  + FC+++ P+     E 
Sbjct: 368 CYHGRVSEELIGFPVVTFHFAGGAELAMEATSMFYPLSEPNTFNVFCMSVKPTKEHGGEY 427

Query: 433 LNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456
              + IG++AQQ YN+G+DL++  ++ +RIDC  LD+
Sbjct: 428 KEFTAIGLMAQQYYNIGYDLKEKNIYLQRIDCVQLDD 453

[Close]

BLAST of CSPI01G20490 vs. TAIR 10
Match: AT4G30040.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 272.3 bits (695), Expect = 7.0e-73
Identity = 161/388 (41.49%), Postives = 220/388 (56.70%), Query Frame = 0


Query: 68  SSIERFGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLL 127
           +S+ER  +L++K              +IP      FLVN+SIGSPP+TQL+ +DT S LL
Sbjct: 54  ASVERLEYLKAKTTGDIIAHLSPNVPIIP----QAFLVNISIGSPPITQLLHMDTASDLL 113

Query: 128 WVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEYKLRYLGGD 187
           W+QCLPCINC+ QS   FDP +S + +   C    Y+  +          EY +RY+   
Sbjct: 114 WIQCLPCINCYAQSLPIFDPSRSYTHRNETCRTSQYSMPSLKFNANTRSCEYSMRYVDDT 173

Query: 188 SSQGILAKESLLFETL--DEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPHITMA 247
            S+GILA+E LLF T+  +       +V FGCGH N          G+ GLG Y   ++ 
Sbjct: 174 GSKGILAREMLLFNTIYDESSSAALHDVVFGCGHDNY--GEPLVGTGILGLG-YGEFSLV 233

Query: 248 TQLGNKFSYCIGDINNPLYTHNHLVLG-QGSYIEGDSTPLQIHFGHYYVTLQSISVGSKT 307
            + G KFSYC G +++P Y HN LVLG  G+ I GD+TPL+IH G YYVT+++ISV    
Sbjct: 234 HRFGKKFSYCFGSLDDPSYPHNVLVLGDDGANILGDTTPLEIHNGFYYVTIEAISVDGII 293

Query: 308 LKIDPNAF-KISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR--- 367
           L IDP  F +    G GG +ID+G + T L    ++ L + I D+ +G        +   
Sbjct: 294 LPIDPRVFNRNHQTGLGGTIIDTGNSLTSLVEEAYKPLKNRIEDIFEGRFTAADVSQDDM 353

Query: 368 -KFEGLCFKGVVSRDLV--GFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSE 427
            K E  C+ G   RDLV  GFP VTFHF+ GA+L L+  SLF +   + FCLA+ P    
Sbjct: 354 IKME--CYNGNFERDLVESGFPIVTFHFSEGAELSLDVKSLFMKLSPNVFCLAVTPG--- 413

Query: 428 LLNLSVIGILAQQNYNVGFDLEQMKVFF 446
             NL+ IG  AQQ+YN+G+DLE M+V F
Sbjct: 414 --NLNSIGATAQQSYNIGYDLEAMEVSF 427

[Close]

BLAST of CSPI01G20490 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 186.0 bits (471), Expect = 6.6e-47
Identity = 140/461 (30.37%), Postives = 232/461 (50.33%), Query Frame = 0


Query: 14  LILAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVEDRSKREQTSSIERF 73
           ++L F+L  ++  S+  +  P   + +LIHR+S L P+Y+   TV DR       S+ R 
Sbjct: 5   ILLCFFLFFSVTLSS--SGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS 64

Query: 74  GFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLP 133
                ++ +      + +S LI       F ++++IG+PP+    + DTGS L WVQC P
Sbjct: 65  RRFNHQLSQ-----TDLQSGLI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKP 124

Query: 134 CINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYK--CNRFNQAEYKLRYLGGDS--S 193
           C  C++++   FD  KS ++K+  C       ++  +  C+  N    K RY  GD   S
Sbjct: 125 CQQCYKENGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNI-CKYRYSYGDQSFS 184

Query: 194 QGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPHITMATQLG 253
           +G +A E++  ++     +      FGCG+ N  T  D+  +G+ GLG   H+++ +QLG
Sbjct: 185 KGDVATETVSIDSASGSPVSFPGTVFGCGYNNGGT-FDETGSGIIGLGG-GHLSLISQLG 244

Query: 254 N----KFSYCIGDINNPLYTHNHLVLGQG-----SYIEGD----STPL--QIHFGHYYVT 313
           +    KFSYC+   +    T+   V+  G     S +  D    STPL  +    +YY+T
Sbjct: 245 SSISKKFSYCLS--HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLT 304

Query: 314 LQSISVGSKTL-----KIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLM 373
           L++ISVG K +       +PN   I S+ SG ++IDSG T T L  G F+     + + +
Sbjct: 305 LEAISVGKKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESV 364

Query: 374 KGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCL 433
            G  +R+   +     CFK   +   +G P +T HF  GAD+ L   + F +   D  CL
Sbjct: 365 TG-AKRVSDPQGLLSHCFKSGSAE--IGLPEITVHFT-GADVRLSPINAFVKLSEDMVCL 424

Query: 434 AILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 451
           +++P+      +++ G  AQ ++ VG+DLE   V F+ +DC
Sbjct: 425 SMVPTT----EVAIYGNFAQMDFLVGYDLETRTVSFQHMDC 443

[Close]

BLAST of CSPI01G20490 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 183.3 bits (464), Expect = 4.3e-46
Identity = 141/457 (30.85%), Postives = 212/457 (46.39%), Query Frame = 0


Query: 11  LPSLILAFYLSTAIISSTLITTKPSR----LATKLIHRNSYLHPLYDQNETVEDRSKREQ 70
           + SL  +  LS  ++SS  ++   ++        LIHR+S   P Y+  ET   R +   
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAI 60

Query: 71  TSSIER-FGFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSS 130
             S+ R F F E          N  +  +   +    +L+N+SIG+PP   + + DTGS 
Sbjct: 61  HRSVNRVFHFTEK--------DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSD 120

Query: 131 LLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYI-NGYKCN-RFNQAEYKLRY 190
           LLW QC PC +C+ Q    FDP  S ++K + C       + N   C+   N   Y L Y
Sbjct: 121 LLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSY 180

Query: 191 LGGDSSQGILAKESLLFETLDEGKIKKSNVTFGCGHMNIKTNNDDAYNGVFGLGAYPHIT 250
                ++G +A ++L   + D   ++  N+  GCGH N  T N    +G+ GLG  P ++
Sbjct: 181 GDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKG-SGIVGLGGGP-VS 240

Query: 251 MATQLGN----KFSYCIGDINNPLYTHNHLVLGQGSYIEGD---STPLQIHFGH---YYV 310
           +  QLG+    KFSYC+  + +     + +  G  + + G    STPL         YY+
Sbjct: 241 LIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYL 300

Query: 311 TLQSISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLL 370
           TL+SISVGSK ++   +    S    G ++IDSG T T L        Y E+ D +   +
Sbjct: 301 TLKSISVGSKQIQYSGSD---SESSEGNIIIDSGTTLTLLPTE----FYSELEDAVASSI 360

Query: 371 ERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILP 430
           +    Q    GL      + DL   P +T HF  GAD+ L+S + F Q   D  C A   
Sbjct: 361 DAEKKQDPQSGLSLCYSATGDL-KVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAFRG 420

Query: 431 SNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 451
           S S     S+ G +AQ N+ VG+D     V F+  DC
Sbjct: 421 SPS----FSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 434

The following BLAST results are available for this feature:

BLAST of CSPI01G20490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3EBM5	9.2e-46	30.37	Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561...	[more]
Q6XBF8	6.0e-45	30.85	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q766C3	7.3e-43	33.99	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	4.4e-40	28.70	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9SV77	4.2e-38	31.11	Aspartyl protease UND OS=Arabidopsis thaliana OX=3702 GN=UND PE=2 SV=1	[more]

BLAST of CSPI01G20490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUP5	6.9e-262	99.34	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G42435...	[more]
A0A5D3DZ20	2.0e-253	96.70	Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 ...	[more]
A0A5A7UU11	2.0e-253	96.70	Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]
A0A1S3BY28	2.3e-249	92.99	aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103494349 PE=3 SV=1	[more]
A0A6J1C0L1	2.6e-224	83.70	probable aspartic protease At2g35615 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]

BLAST of CSPI01G20490 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031742409.1	1.4e-261	99.34	aspartic proteinase CDR1 [Cucumis sativus] >KGN65478.1 hypothetical protein Csa_...	[more]
KAA0058227.1	4.2e-253	96.70	aspartic proteinase CDR1-like [Cucumis melo var. makuwa]	[more]
TYK28585.1	4.2e-253	96.70	Peptidase A1 [Cucumis melo var. makuwa]	[more]
XP_008453701.1	4.8e-249	92.99	PREDICTED: aspartic proteinase CDR1-like [Cucumis melo]	[more]
XP_038878960.1	3.8e-238	89.23	aspartic proteinase CDR1-like isoform X1 [Benincasa hispida]	[more]

BLAST of CSPI01G20490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30030.1	9.4e-86	42.17	Eukaryotic aspartyl protease family protein	[more]
AT2G23945.1	1.4e-78	39.17	Eukaryotic aspartyl protease family protein	[more]
AT4G30040.1	7.0e-73	41.49	Eukaryotic aspartyl protease family protein	[more]
AT2G35615.1	6.6e-47	30.37	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	4.3e-46	30.85	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 290..446 e-value: 6.1E-30 score: 104.1
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 103..271 e-value: 8.5E-33 score: 114.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 85..272 e-value: 7.5E-36 score: 125.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 277..453 e-value: 2.1E-41 score: 143.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 101..451
None	No IPR available	PANTHER	PTHR47967:SF14	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 26..452
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 26..452
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 118..129
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 103..446 score: 31.791201
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 102..450 e-value: 3.98848E-71 score: 224.064

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G20490.1	CSPI01G20490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0016757	glycosyltransferase activity