CSPI01G19410 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G19410
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRibonuclease H
LocationChr1: 14926049 .. 14928824 (-)
RNA-Seq ExpressionCSPI01G19410
SyntenyCSPI01G19410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAGAATGCAAGCCAGTAAGACAAAAGTTACGTAAAATGAAGTCTGATGTATTGATTAAAATAAAAGAGAAAGTACAGAAACAAATTGAAGCAGAGTTCATCACAGTCTCCAAATATCCTGAATGGGTGGCAAACATTGTTCCAGTACACAAAAAAGACAAAAAAGTGAGAATGTGTGTGAACTATAGAAATTTAAACCGAGTTAGTCCAAAAGACAACTTCCCTTTACCTCATATTGACATGTTGGTGGATAACACTACATGATACTTAACTTTCTCCTTCATGGATGGTTTTTCAAGATATAATCAAATCAAATAAGCTGAAAAAAATAGAGAAAAAACAACATTCATTACCCTTTGGGGAACATTCTGCTACAAAGTAATGCCTTTTGATTTGAAAAATACTGGGACAACGTATCAGCGAGCAATGATTACATTTTTTCATGATATGATGCATAAGGAAATAGAGGTGTATGTTAATGATATGATTGCAAAATCCAAGAACAATGAATATCATACAACTATACTCCAAAAATTATTTAATTGGTTGACAAAGAATCAATTGAAATTAAATCCATCCAAATGTACATTTGGGGCAACCTAGGGAAAGCTGTTGGGATTCATTGTCAGTGAAGAAGGGATCAAAGTAGACCTAGATAAAGTGAAGGCTATCATGGAAATACCATCCCCTGAAACAGAAAAAGAAGTTTGAGGTCTTATTGGAAGATTGAACTACATATCCAGATTTATCTCGCATTTGACACCAGCATGCGAACTAATCTTCAAGCTTTTACGTAAAAACAATCCAGAAAAATGGAATGAGGATTGTGAAGAAGCTTTCAACAAAATTAAGCAGTATTTGTAGAGCCCACCTGTATTGATACCTTCAACTCCAGGGTGACCGTTAATCTTATACTTGACAGTATTAGAAGGATCTATGAGTGGTGTTCTAGGACAACATGACTTATCAGGGAAGAAAGAACACGCTATTTATTATTTGAGCAAAAAATTCACCGATTATGAGTCGAAATACTCAATGTTAGAGCGAACTTGTTGTTCTTACTTCAAAAAATTCACCGTTTGAGGCAATATATGTTATATCATACGACATGGCTTATTTCAAAAATGGATCCAATAAAATACAATTTTTAGAAATCGTCATTATTTGGGAGAATTGCAAAATGAAAAATTTTATTGTCAGAGTATGATATTGTTTATGTTACTAAAAGAGCAATAAAGGGAAGCGCAATTGCCGATCATTTAGCTGCCCAACTAGTAGTAGATTACGAACCAATAAGTGTTGATTTCCCTGATGAAAACATATTTTTAGTTGAAAAGGATGCTACAGATCATGAGACATGGATTATGCTTTTTGATGGTGCCTCAAATGAGTTGGGACATGGGATTAGAGTTGCACTAATTTTCCCAGAAGGAAAGGTATTTCCCCTAATAGCTAAGTTATGTTTTGAATGCACTCACAATATCGTTGAATATGAAGCCTGTATTATGGGACTTCAAGCAGTAATCGACATGAGTCTTAAAAAGTTGAAAGTTTTGGGTGACTCAATGCTAGTAATACACCGAGTCAAGGAAGAATGGGAAACAAGAGATGCTAAATTGGTGCCTTACAGCCAATACGTTGCAAAATTATCTCAAAATTTTGAGAAAATTTCATTTGACCATGTTCATAAGGAAGACAATTGGATGACAGATGCATTAGCCACCCTGGCAGTAATGTTTAATCTTAACCTTGAGTGTGAACTTTATCCGATCCAAATTACAAAGCAAGATGTGTCAGCATATTGCATGAATATTATAAATGATAATAAATCATGATATTTGGATATCAAGCAATACATAAAGTGTAGATAATATTCATATGGAGCTCCAGAGAATAACAAGCGCACAATAAGAAGGTTGGCCATGAACTTTTTCTTAAGTGGTAAAGTTCTTTATAAAAGAAATCATGATATGGTGCTCCTTCGATGTGTTTGATGTGGAAGAAGCAAAACAAATTATGACAGAAATTCATGAAGGAATATGTGGAACACATGCAAGGGAATGTAAAAAAATACTTAGATTTGGTTATTATTGGACAACGATGGAATCAGACTGCATAAAATATACAAGGGAATGTAAAAAGTGTCAAATTTATATGGATAAGATCCGTGCAGCAACATCTTCATTGCATGTCTTGTCAGCACCCTGGCCGTTTTCTTTGTAGGGAATGGTTGCTATTGGACCCATTGATCCTAAAGCCTCAAATGATCACCGTTTTATTCTTGTAGCCATTGATCACTTCACTAAATGGATAGAGGCGGCATCTTACTACGATGTTACAAGAGGGGTGGTGCTCAAGTTTATCAAGAAAGAGTTGATCTATCGTTATGGTCTTCCAGAGGGTATTATTATAGATAATGCCAAGAATCTTAATAACAAAATGATGGACCAACTTTTATGGGCAATTCAAGATAAATCACAGAAATTCGACTCCATATCACCCCAACATGAATGGGGCAATTGAGACAGCCAACAAAAATATTAAAAAAATCATTGGGAAGATGACAACAACATACAAAGATTGGCATGAAATTTTACCGTTTGCGCTGCATGGATATCGCACATCAGTTCGTGCTTCAACAAGGACAACACCATTTTCTTTGGTTTATGGTATGGAAGTTGTCCTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAATCAATTGTATACGAGGTCGTTATGTGCAGTTGA

mRNA sequence

CCAGAATGCAAGCCAGTAAGACAAAAGTTACGTAAAATGAAGTCTGATGTATTGATTAAAATAAAAGAGAAAGTACAGAAACAAATTGAAGCAGAGTTCATCACAGTCTCCAAATATCCTGAATGGGTGGCAAACATTGTTCCAGGAAAGCTGTTGGGATTCATTGTCAGTGAAGAAGGGATCAAAGTAGACCTAGATAAAGTGAAGGCTATCATGGAAATACCATCCCCTGAAACAGAAAAAGAAGATTGTGAAGAAGCTTTCAACAAAATTAAGCATGGTGTTCTAGGACAACATGACTTATCAGGGAAGAAAGAACACGCTATTTATTATTTGAGCAAAAAATTCACCGATTATGAGTCGAAATACTCAATGTTAGAGCGAACTTGTTGTTCTTACTTCAAAAAATTCACCTATGATATTGTTTATGTTACTAAAAGAGCAATAAAGGGAAGCGCAATTGCCGATCATTTAGCTGCCCAACTAGTAGTAGATTACGAACCAATAAGTGTTGATTTCCCTGATGAAAACATATTTTTAGTTGAAAAGGATGCTACAGATCATGAGACATGGATTATGCTTTTTGATGGTGCCTCAAATGAGTTGGGACATGGGATTAGAGTTGCACTAATTTTCCCAGAAGGAAAGGTATTTCCCCTAATAGCTAAGTTATGTTTTGAATGCACTCACAATATCGTTGAATATGAAGCCTGTATTATGGGACTTCAAGCAGTAATCGACATGAGTCTTAAAAAGTTGAAAGTTTTGGGTGACTCAATGCTAGTAATACACCGAGTCAAGGAAGAATGGGAAACAAGAGATGCTAAATTGGTGCCTTACAGCCAATACGTTGCAAAATTATCTCAAAATTTTGAGAAAATTTCATTTGACCATGTTCATAAGGAAGACAATTGGATGACAGATGCATTAGCCACCCTGGCAGTAATGTTTAATCTTAACCTTGAGTGTGAACTTTATCCGATCCAAATTACAAAGCAAGATAATAACAAGCGCACAATAAGAAGGTTGGCCATGAACTTTTTCTTAAGTGGTAAAGTTCTTTATAAAAGAAATCATGATATGGTGCTCCTTCGATGTTGTCAAATTTATATGGATAAGATCCGTGCAGCAACATCTTCATTGCATGGAATGGTTGCTATTGGACCCATTGATCCTAAAGCCTCAAATGATCACCGTTTTATTCTTGTAGCCATTGATCACTTCACTAAATGGATAGAGGCGGCATCTTACTACGATGTTACAAGAGGGGTGATAAATCACAGAAATTCGACTCCATATCACCCCAACATGAATGGGGCAATTGAGACAGCCAACAAAAATATTAAAAAAATCATTGGGAAGATGACAACAACATACAAAGATTGGCATGAAATTTTACCGTTTGCGCTGCATGGATATCGCACATCAGTTCGTGCTTCAACAAGGACAACACCATTTTCTTTGGTTTATGGTATGGAAGTTGTCCTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAATCAATTGTATACGAGGTCGTTATGTGCAGTTGA

Coding sequence (CDS)

CCAGAATGCAAGCCAGTAAGACAAAAGTTACGTAAAATGAAGTCTGATGTATTGATTAAAATAAAAGAGAAAGTACAGAAACAAATTGAAGCAGAGTTCATCACAGTCTCCAAATATCCTGAATGGGTGGCAAACATTGTTCCAGGAAAGCTGTTGGGATTCATTGTCAGTGAAGAAGGGATCAAAGTAGACCTAGATAAAGTGAAGGCTATCATGGAAATACCATCCCCTGAAACAGAAAAAGAAGATTGTGAAGAAGCTTTCAACAAAATTAAGCATGGTGTTCTAGGACAACATGACTTATCAGGGAAGAAAGAACACGCTATTTATTATTTGAGCAAAAAATTCACCGATTATGAGTCGAAATACTCAATGTTAGAGCGAACTTGTTGTTCTTACTTCAAAAAATTCACCTATGATATTGTTTATGTTACTAAAAGAGCAATAAAGGGAAGCGCAATTGCCGATCATTTAGCTGCCCAACTAGTAGTAGATTACGAACCAATAAGTGTTGATTTCCCTGATGAAAACATATTTTTAGTTGAAAAGGATGCTACAGATCATGAGACATGGATTATGCTTTTTGATGGTGCCTCAAATGAGTTGGGACATGGGATTAGAGTTGCACTAATTTTCCCAGAAGGAAAGGTATTTCCCCTAATAGCTAAGTTATGTTTTGAATGCACTCACAATATCGTTGAATATGAAGCCTGTATTATGGGACTTCAAGCAGTAATCGACATGAGTCTTAAAAAGTTGAAAGTTTTGGGTGACTCAATGCTAGTAATACACCGAGTCAAGGAAGAATGGGAAACAAGAGATGCTAAATTGGTGCCTTACAGCCAATACGTTGCAAAATTATCTCAAAATTTTGAGAAAATTTCATTTGACCATGTTCATAAGGAAGACAATTGGATGACAGATGCATTAGCCACCCTGGCAGTAATGTTTAATCTTAACCTTGAGTGTGAACTTTATCCGATCCAAATTACAAAGCAAGATAATAACAAGCGCACAATAAGAAGGTTGGCCATGAACTTTTTCTTAAGTGGTAAAGTTCTTTATAAAAGAAATCATGATATGGTGCTCCTTCGATGTTGTCAAATTTATATGGATAAGATCCGTGCAGCAACATCTTCATTGCATGGAATGGTTGCTATTGGACCCATTGATCCTAAAGCCTCAAATGATCACCGTTTTATTCTTGTAGCCATTGATCACTTCACTAAATGGATAGAGGCGGCATCTTACTACGATGTTACAAGAGGGGTGATAAATCACAGAAATTCGACTCCATATCACCCCAACATGAATGGGGCAATTGAGACAGCCAACAAAAATATTAAAAAAATCATTGGGAAGATGACAACAACATACAAAGATTGGCATGAAATTTTACCGTTTGCGCTGCATGGATATCGCACATCAGTTCGTGCTTCAACAAGGACAACACCATTTTCTTTGGTTTATGGTATGGAAGTTGTCCTACCTTTAGAAGTTGAGATACCTTCATTGAGAGTTCTCATGGAAGCTAAGTTAGATGAATCAATTGTATACGAGGTCGTTATGTGCAGTTGA

Protein sequence

PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVPGKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEKEDCEEAFNKIKHGVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCSYFKKFTYDIVYVTKRAIKGSAIADHLAAQLVVDYEPISVDFPDENIFLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEACIMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFDHVHKEDNWMTDALATLAVMFNLNLECELYPIQITKQDNNKRTIRRLAMNFFLSGKVLYKRNHDMVLLRCCQIYMDKIRAATSSLHGMVAIGPIDPKASNDHRFILVAIDHFTKWIEAASYYDVTRGVINHRNSTPYHPNMNGAIETANKNIKKIIGKMTTTYKDWHEILPFALHGYRTSVRASTRTTPFSLVYGMEVVLPLEVEIPSLRVLMEAKLDESIVYEVVMCS*
Homology
BLAST of CSPI01G19410 vs. ExPASy Swiss-Prot
Match: P27401 (Pro-Pol polyprotein OS=Simian foamy virus type 3 (strain LK3) OX=11644 GN=pol PE=3 SV=2)

HSP 1 Score: 48.1 bits (113), Expect = 3.5e-04
Identity = 22/69 (31.88%), Postives = 38/69 (55.07%), Query Frame = 0

Query: 430  STPYHPNMNGAIETANKNIKKIIGKMTT-TYKDWHEILPFALHGYRTSVRASTRTTPFSL 489
            STPYHP  +G +E  N +IK+++ K+       W+++LP        S   S++ TP  L
Sbjct: 959  STPYHPQSSGKVERKNSDIKRLLTKLLVGRPAKWYDLLPVVQLALNNSYSPSSKYTPHQL 1018

Query: 490  VYGMEVVLP 498
            ++G++   P
Sbjct: 1019 LFGIDSNTP 1027

BLAST of CSPI01G19410 vs. ExPASy Swiss-Prot
Match: P23074 (Pro-Pol polyprotein OS=Simian foamy virus type 1 OX=338478 GN=pol PE=1 SV=3)

HSP 1 Score: 47.8 bits (112), Expect = 4.5e-04
Identity = 22/69 (31.88%), Postives = 38/69 (55.07%), Query Frame = 0

Query: 430  STPYHPNMNGAIETANKNIKKIIGKMTT-TYKDWHEILPFALHGYRTSVRASTRTTPFSL 489
            STPYHP  +G +E  N +IK+++ K+       W+++LP        S   S++ TP  L
Sbjct: 959  STPYHPQSSGKVERKNSDIKRLLTKLLIGRPAKWYDLLPVVQLALNNSYSPSSKYTPHQL 1018

Query: 490  VYGMEVVLP 498
            ++G++   P
Sbjct: 1019 LFGVDSNTP 1027

BLAST of CSPI01G19410 vs. ExPASy TrEMBL
Match: A0A5A7T7R4 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002820 PE=4 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 3.1e-197
Identity = 434/887 (48.93%), Postives = 467/887 (52.65%), Query Frame = 0

Query: 1    PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVP------------ 60
            PEC PVRQKLRKMK DVLIKIKE+VQKQIEA F+TVSKYPEWVAN+VP            
Sbjct: 944  PECNPVRQKLRKMKPDVLIKIKEEVQKQIEAGFLTVSKYPEWVANVVPVPKKDGKGRMIL 1003

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 1004 ISFMDGFSGYNQIKMAKEDREKTTFITLWGTFCYKVMPFGLKNDGATYQRPMVTLFHDMM 1063

Query: 121  --------------------------------------------------GKLLGFIVSE 180
                                                              GKLLGFIVSE
Sbjct: 1064 HKEIEVYVDDMIAKSKVDEDHTTTLQKLFDRLRKYQLKLNPSKCIFGATSGKLLGFIVSE 1123

Query: 181  EGIKVDLDKVKAIMEIPSPETEK------------------------------------- 240
            EGIKVD DKV+AIME+PSP+TEK                                     
Sbjct: 1124 EGIKVDPDKVRAIMEMPSPKTEKEIRGFLGRLNYISRFISHLTPTCEPIFKLLHKNNPGK 1183

Query: 241  --EDCEEAFNKIKH-----------------------------GVLGQHDLSGKKEHAIY 300
              EDCEEAFNKIK                              GVLGQHDLSGKK+H IY
Sbjct: 1184 WNEDCEEAFNKIKQYLQSPPVLMPPAPGRPLILYLTVLESSMGGVLGQHDLSGKKKHVIY 1243

Query: 301  YLSKKFTDYESKYSMLERTCCS----------------------------YFKK------ 360
            YLSKKFT+YES+YSMLERTCC+                             F+K      
Sbjct: 1244 YLSKKFTNYESRYSMLERTCCTLVWTAHRLRQYMLYHTTCLISKMDPIKYIFEKPSLSGR 1303

Query: 361  --------FTYDIVYVTKRAIKGSAIADHLAAQLVVDYEPISVDFPDENIFLVEKDATDH 420
                      YDIVYVTK+AIK SA+ADHLAAQ V DYEP+ +DFPD+NIFLVEK+A DH
Sbjct: 1304 IAKWQVLLSEYDIVYVTKKAIKESAVADHLAAQPVADYEPMRIDFPDDNIFLVEKNARDH 1363

Query: 421  ETWIMLFDGASNELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEACIMGLQAVIDM 480
            ETW MLFDGASNELGH I V LI PEGKVFPL AKLCFECTHNI EYEACIMGLQ   DM
Sbjct: 1364 ETWTMLFDGASNELGHEIGVVLISPEGKVFPLTAKLCFECTHNIAEYEACIMGLQVACDM 1423

Query: 481  SLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFDHVHKEDNWMTD 517
            S+KKLKVLGDSMLVIH+VKEEWETR AKLVPYSQYV KLSQNFEKISFDHV +EDN M D
Sbjct: 1424 SIKKLKVLGDSMLVIHQVKEEWETRHAKLVPYSQYVTKLSQNFEKISFDHVPREDNRMAD 1483

BLAST of CSPI01G19410 vs. ExPASy TrEMBL
Match: A0A5A7T0A1 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00070 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 4.3e-191
Identity = 400/717 (55.79%), Postives = 425/717 (59.27%), Query Frame = 0

Query: 49  GKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEK--------------------------- 108
           GKLLGFIVSEEGIKVD DKV+AIME+PSP+TEK                           
Sbjct: 60  GKLLGFIVSEEGIKVDPDKVRAIMEMPSPKTEKEIRGFLGRLNYISRFISHLTPTCEPIF 119

Query: 109 ------------EDCEEAFNKIKH-----------------------------GVLGQHD 168
                       EDCEEAFNKIK                              GVLGQHD
Sbjct: 120 KLLRKNNPGKWNEDCEEAFNKIKQYLQSPPVLIPPAPGRPLILYLTVLESSVDGVLGQHD 179

Query: 169 LSGKKEHAIYYLSKKFTDYESKYSMLERTCCS---------------------------- 228
           LSGKKEHAIYYLSKKFTDYES+YSMLERTCC+                            
Sbjct: 180 LSGKKEHAIYYLSKKFTDYESRYSMLERTCCALVWTAHHLRQYMLYHTTCLISKMDPIKY 239

Query: 229 YFKK--------------FTYDIVYVTKRAIKGSAIADHLAAQLVVDYEPISVDFPDENI 288
            F+K                YDIVYVTK+AIKGSA+ADHLAAQ V DYEP+ +DF DENI
Sbjct: 240 IFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSAVADHLAAQPVADYEPMRIDFLDENI 299

Query: 289 FLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEAC 348
           FLVEK+A DHETW MLFDGASNELGHGI V LI  EGKVF L AKLCFECTHNI EYEAC
Sbjct: 300 FLVEKNARDHETWTMLFDGASNELGHGIGVVLISSEGKVFSLTAKLCFECTHNIAEYEAC 359

Query: 349 IMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFDH 408
           IMGLQ   DMS+KKLKVLGDSMLVIH+VKEEWE RDAKLVPYSQYV KLSQNFEKISFDH
Sbjct: 360 IMGLQVAWDMSIKKLKVLGDSMLVIHQVKEEWEIRDAKLVPYSQYVTKLSQNFEKISFDH 419

Query: 409 VHKEDNWMTDALATLAVMFNLNLECELYPIQITKQD------------------------ 468
           V +EDN M DALATLAVMF+LNLE EL+PIQITK+D                        
Sbjct: 420 VPREDNRMADALATLAVMFDLNLEFELHPIQITKRDVPAYCMNVGNDNKPWYFDIKQYIK 479

Query: 469 ----------NNKRTIRRLAMNFFLSGKVLYKRNHDMVLLRC------------------ 517
                     N+KRTIRRLAMNFFLSG+VLYKRNHDMVLLRC                  
Sbjct: 480 CREYPYEASENDKRTIRRLAMNFFLSGEVLYKRNHDMVLLRCVDEEEAKQIMTDIYEGIC 539

BLAST of CSPI01G19410 vs. ExPASy TrEMBL
Match: A0A5D3DMD2 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold717G00160 PE=4 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 6.9e-189
Identity = 395/716 (55.17%), Postives = 420/716 (58.66%), Query Frame = 0

Query: 49  GKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEK--------------------------- 108
           GKLLGFIVSEEGIKVD DKV+AIME+PSP+TEK                           
Sbjct: 145 GKLLGFIVSEEGIKVDPDKVRAIMEMPSPKTEKEIRGFLGRLNYISRFISYLTPTCEPIF 204

Query: 109 ------------EDCEEAFNKIKH-----------------------------GVLGQHD 168
                       EDCEEAFNKIK                              GVLGQHD
Sbjct: 205 KLLRKNNPGKWNEDCEEAFNKIKQYLQSPPVLIPPAPGRPLILYLTVLESSMGGVLGQHD 264

Query: 169 LSGKKEHAIYYLSKKFTDYESKYSMLERTCCS---------------------------- 228
           LSGKKEHAIYYLSKKFTDYES+YSMLERTCC+                            
Sbjct: 265 LSGKKEHAIYYLSKKFTDYESRYSMLERTCCALVWTAHRLRQYMLYHTTCLISKMDRIKY 324

Query: 229 YFKK--------------FTYDIVYVTKRAIKGSAIADHLAAQLVVDYEPISVDFPDENI 288
            F+K                YDIVYVTK+AIKGSA+ADHLAAQ V DYEP+ +DFPD+NI
Sbjct: 325 IFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSAVADHLAAQPVADYEPMRIDFPDDNI 384

Query: 289 FLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEAC 348
           FLVEK+A DHETW MLFDGASNELGHGI V LI PEGKVFPL AKLCFECTHNI EYEAC
Sbjct: 385 FLVEKNARDHETWTMLFDGASNELGHGIGVVLISPEGKVFPLTAKLCFECTHNIAEYEAC 444

Query: 349 IMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFDH 408
           IMGLQ   DMS+KKLKVLGDSMLVIH+VKEEWETRDAKLVPYSQYV KLSQNFEKISFDH
Sbjct: 445 IMGLQVACDMSIKKLKVLGDSMLVIHQVKEEWETRDAKLVPYSQYVTKLSQNFEKISFDH 504

Query: 409 VHKEDNWMTDALATLAVMFNLNLECELYPIQITKQD------------------------ 468
           V +EDN M DALATLAVMF+LNLE EL+PIQITK+D                        
Sbjct: 505 VPREDNRMADALATLAVMFDLNLEFELHPIQITKRDVPAYCMNVGNDNKPWYFDIKQYIK 564

Query: 469 ----------NNKRTIRRLAMNFFLSGKVLYKRNHDMVLLRC------------------ 516
                     N+KR IRRL MNFFLSG+VLYKRNHDMVLLRC                  
Sbjct: 565 CREYPYEASENDKRAIRRLTMNFFLSGEVLYKRNHDMVLLRCVDEEEAKQIMTDIHEGIC 624

BLAST of CSPI01G19410 vs. ExPASy TrEMBL
Match: A0A5D3BAL8 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10G00460 PE=4 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 9.3e-178
Identity = 386/744 (51.88%), Postives = 412/744 (55.38%), Query Frame = 0

Query: 1    PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVP------------ 60
            PEC PVRQKLRKMK +VLIKIKE+VQKQIEA F+TVSKYPEWV NIVP            
Sbjct: 549  PECNPVRQKLRKMKLNVLIKIKEEVQKQIEAGFLTVSKYPEWVTNIVPVPKKDRKDTQLS 608

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 609  PSWMIFQDIIRSKWLKKIEKKRHSLPFGKHSAKKIEVYVVNMIAKSKADEDHTTTLQKLF 668

Query: 121  ---------------------GKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEKEDCEEA 180
                                 GKLLGFIVSEEGIKVD DKV+AIME+PSP+TEKE  E  
Sbjct: 669  DRLRKYQLKLNPSKCTFGETSGKLLGFIVSEEGIKVDPDKVRAIMEMPSPKTEKEIRE-- 728

Query: 181  FNKIKHGVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCSYFKKFTYDIVYVTKR 240
                                                               YDIVYVTK+
Sbjct: 729  ---------------------------------------------------YDIVYVTKK 788

Query: 241  AIKGSAIADHLAAQLVVDYEPISVDFPDENIFLVEKDATDHETWIMLFDGASNELGHGIR 300
            AIKGSAIADHLAAQ V DYEP+ +DFPDENIF VEK+A DHETW MLFDGASNELGHGI 
Sbjct: 789  AIKGSAIADHLAAQPVADYEPMRIDFPDENIFRVEKNARDHETWTMLFDGASNELGHGIG 848

Query: 301  VALIFPEGKVFPLIAKLCFECTHNIVEYEACIMGLQAVIDMSLKKLKVLGDSMLVIHRVK 360
            V LI PEGKVFPL AKLCFECTHNI EY+ACIMGLQ   DMS+KKLKVLGDSMLVIH+VK
Sbjct: 849  VVLISPEGKVFPLTAKLCFECTHNIAEYKACIMGLQVACDMSIKKLKVLGDSMLVIHQVK 908

Query: 361  EEWETRDAKLVPYSQYVAKLSQNFEKISFDHVHKEDNWMTDALATLAVMFNLNLECELYP 420
            EEWETRDAKLVPYSQYV KLSQNFEKISFDHV +EDN + DALATLAVMF+LNLE EL  
Sbjct: 909  EEWETRDAKLVPYSQYVTKLSQNFEKISFDHVPREDNRIADALATLAVMFDLNLEFELLQ 968

Query: 421  IQITKQD----------------------------------NNKRTIRRLAMNFFLSGKV 480
            IQITK+D                                  N+KRTIRRLA+NFFLSG+V
Sbjct: 969  IQITKRDVPAYCMNVGNDNKPWYFDIKQYIKCREYPYEALENDKRTIRRLAINFFLSGEV 1028

Query: 481  LYKRNHDMVLLRC----------------------------------------------- 513
            LYKRNHDMVLLRC                                               
Sbjct: 1029 LYKRNHDMVLLRCVDKEKAKQIMTDIHEGICGTHANGHMMARQILRSGYYWTTMESDCIK 1088

BLAST of CSPI01G19410 vs. ExPASy TrEMBL
Match: A0A5A7UKG2 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G001050 PE=4 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 1.5e-172
Identity = 364/654 (55.66%), Postives = 400/654 (61.16%), Query Frame = 0

Query: 1    PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVPGKLLGFIVSEEG 60
            PEC PVRQKLRKMK +VLIKIKE+VQKQIEA F+TVSKYPEWV NIV             
Sbjct: 549  PECNPVRQKLRKMKLNVLIKIKEEVQKQIEAGFLTVSKYPEWVTNIV------------- 608

Query: 61   IKVDLDKVKAIMEIPSPETEKEDCEEAFN-KIKHGVLGQHDLS--GKKEHAIYYLSKKFT 120
                          P P+ +++D + + +  I   ++    L    KK H++      F 
Sbjct: 609  --------------PVPKKDRKDTQLSPSWMIFQDIIRSKWLKKIEKKRHSL-----PFG 668

Query: 121  DYESKYSMLERTCCSYFKKFTYDIVYVTKRAIKGSAIADHLAAQLVVDYEPISVDFPDEN 180
             + +K                YDIVYVTK+AIKGSAIADHLAAQ V DYEP+ +DFPDEN
Sbjct: 669  KHSAK---------------KYDIVYVTKKAIKGSAIADHLAAQPVADYEPMRIDFPDEN 728

Query: 181  IFLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEA 240
            IF VEK+A DHETW MLFDGASNELGHGI V LI PEGKVFPL AKLCFECTHNI EY+A
Sbjct: 729  IFRVEKNARDHETWTMLFDGASNELGHGIGVVLISPEGKVFPLTAKLCFECTHNIAEYKA 788

Query: 241  CIMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFD 300
            CIMGLQ   DMS+KKLKVLGDSMLVIH+VKEEWETRDAKLVPYSQYV KLSQNFEKISFD
Sbjct: 789  CIMGLQVACDMSIKKLKVLGDSMLVIHQVKEEWETRDAKLVPYSQYVTKLSQNFEKISFD 848

Query: 301  HVHKEDNWMTDALATLAVMFNLNLECELYPIQITKQD----------------------- 360
            HV +EDN + DALATLAVMF+LNLE EL  IQITK+D                       
Sbjct: 849  HVPREDNRIADALATLAVMFDLNLEFELLQIQITKRDVPAYCMNVGNDNKPWYFDIKQYI 908

Query: 361  -----------NNKRTIRRLAMNFFLSGKVLYKRNHDMVLLRC----------------- 420
                       N+KRTIRRLA+NFFLSG+VLYKRNHDMVLLRC                 
Sbjct: 909  KCREYPYEALENDKRTIRRLAINFFLSGEVLYKRNHDMVLLRCVDKEKAKQIMTDIHEGI 968

Query: 421  -------------------------------------CQIYMDKIRAATSSLH------- 480
                                                 CQIYMDKI  A SSLH       
Sbjct: 969  CGTHANGHMMARQILRSGYYWTTMESDCIKYARECKKCQIYMDKIHVAASSLHILSAPWS 1028

Query: 481  ----GMVAIGPIDPKASNDHRFILVAIDHFTKWIEAASYYDVTRGV-------------- 513
                GM  IG IDPKASN HRFILVAID+FTKWIEAASY +VTRGV              
Sbjct: 1029 FSLWGMDVIGAIDPKASNGHRFILVAIDYFTKWIEAASYCNVTRGVMLKFIKKELICRYG 1088

BLAST of CSPI01G19410 vs. NCBI nr
Match: XP_031739260.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116402917 [Cucumis sativus])

HSP 1 Score: 726.9 bits (1875), Expect = 1.3e-205
Identity = 455/915 (49.73%), Postives = 479/915 (52.35%), Query Frame = 0

Query: 1    PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVP------------ 60
            PEC PVRQKLRKMK D+LIKIKE+VQKQIEA F+TVSKYPEWVANIVP            
Sbjct: 1339 PECNPVRQKLRKMKPDILIKIKEEVQKQIEAGFLTVSKYPEWVANIVPVPKKDGKVRMCV 1398

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 1399 DYRDLNRASPKDNFPLPHIDMLVDNTAGYSTFSFMDGFSGYNQIKMAEEDREKTTFITLW 1458

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 1459 GTFCYKVMPFGLKNAGATYQRAMVTLFHDMMHKEIEVYVDDMIAKSKANEDHTTILQKLF 1518

Query: 181  ---------------------GKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEK------ 240
                                 GKLLGFIVSEEGIKVD DKVKAIM++PSPETEK      
Sbjct: 1519 DRLRKYQLKLNPSKCTFGATSGKLLGFIVSEEGIKVDPDKVKAIMDMPSPETEKEVRAFL 1578

Query: 241  ---------------------------------EDCEEAFNKIKH--------------- 300
                                             EDCEEAFNKIK                
Sbjct: 1579 GRLNYISRFISHLTPTCEPIFKLLRKNNPGKWNEDCEEAFNKIKQYLQSPPVLIPPAPGR 1638

Query: 301  --------------GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCS------- 360
                          GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCC+       
Sbjct: 1639 XLILYLTVLEGSMGGVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCALVWTAHR 1698

Query: 361  ---------------------YFKK--------------FTYDIVYVTKRAIKGSAIADH 420
                                  F+K                YDIVYVTK+AIKGSA+ADH
Sbjct: 1699 LRQYMLYHTTWLISKMDPIKYIFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSALADH 1758

Query: 421  LAAQLVVDYEPISVDFPDENIFLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKV 480
            LAAQ V DYEP+SVDFPDENIFLVE DATDHETWIMLFDGASNELGHGI V LI P+GKV
Sbjct: 1759 LAAQPVADYEPMSVDFPDENIFLVENDATDHETWIMLFDGASNELGHGIGVVLISPKGKV 1818

Query: 481  FPLIAKLCFECTHNIVEYEACIMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKL 514
            FPL AKLCFECTHNI EYEACIMGLQA IDMS+KKLKVLGDSMLVIH+VKEEWETRDAKL
Sbjct: 1819 FPLTAKLCFECTHNIAEYEACIMGLQAAIDMSVKKLKVLGDSMLVIHQVKEEWETRDAKL 1878

BLAST of CSPI01G19410 vs. NCBI nr
Match: XP_031741698.1 (uncharacterized protein LOC116403895 [Cucumis sativus])

HSP 1 Score: 725.3 bits (1871), Expect = 3.7e-205
Identity = 454/918 (49.46%), Postives = 480/918 (52.29%), Query Frame = 0

Query: 1    PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVP------------ 60
            PEC PVRQKLRKMK D+LIK+KE+VQKQIEA F+ VSKYPEWVANIVP            
Sbjct: 1277 PECNPVRQKLRKMKPDILIKMKEEVQKQIEAGFLIVSKYPEWVANIVPVPKKDGKMRMCV 1336

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 1337 DYRDLNRASPKDNFPLPHIDMLVDNTAGYSTFSFMDGFSGYNQIKMAEEDREKTTFITLW 1396

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 1397 GTFCYKVMPFGLKNAGATYQRAMVTLFHDMMHKEIEVYVDDMIAKSKANEDHTTILQKLF 1456

Query: 181  ---------------------GKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEK------ 240
                                 GKLLGFIVSEEGIKVD DKVKAIM++PSPETEK      
Sbjct: 1457 DRLRKYQLKLNPSKCTFGATSGKLLGFIVSEEGIKVDPDKVKAIMDMPSPETEKEVRAFL 1516

Query: 241  ---------------------------------EDCEEAFNKIKH--------------- 300
                                             EDCEEAFNKIK                
Sbjct: 1517 GRLNYISRFISHLTPTCEPIFKLLRKNNPGKWNEDCEEAFNKIKQYLQSPPVLIPPAPGR 1576

Query: 301  --------------GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCS------- 360
                          GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCC+       
Sbjct: 1577 PLILYLTVLEGSMGGVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCALVWTAHR 1636

Query: 361  ---------------------YFKK--------------FTYDIVYVTKRAIKGSAIADH 420
                                  F+K                YDIVYVTK+AIKGSA+ADH
Sbjct: 1637 LRQYMLYHTTWLISKMDPIKYIFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSALADH 1696

Query: 421  LAAQLVVDYEPISVDFPDENIFLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKV 480
            LAAQ V DYEP+SVDFPDENIFLVE DATDHETWIMLFDGASNELGHGI V LI P+GKV
Sbjct: 1697 LAAQPVADYEPMSVDFPDENIFLVENDATDHETWIMLFDGASNELGHGIGVVLISPKGKV 1756

Query: 481  FPLIAKLCFECTHNIVEYEACIMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKL 517
            FPL AKLCFECTHNI EYEACIMGLQA IDMS+KKLKVLGDSMLVIH+VKEEWETRDAKL
Sbjct: 1757 FPLTAKLCFECTHNIAEYEACIMGLQAAIDMSVKKLKVLGDSMLVIHQVKEEWETRDAKL 1816

BLAST of CSPI01G19410 vs. NCBI nr
Match: XP_031737561.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116402452 [Cucumis sativus])

HSP 1 Score: 724.9 bits (1870), Expect = 4.9e-205
Identity = 454/917 (49.51%), Postives = 479/917 (52.24%), Query Frame = 0

Query: 1    PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVP------------ 60
            PEC PVRQKLRKMK D+LIK KE+VQKQIEA F+ VSKYPEWVANIVP            
Sbjct: 1266 PECNPVRQKLRKMKPDILIKXKEEVQKQIEAGFLIVSKYPEWVANIVPVPKKDGKVRMCV 1325

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 1326 DYRDLNRASPKDNFPLPHIDMLVDNTAGYSTFSFMDGFSGYNQIKMAEEDREKTTFITLW 1385

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 1386 GTFCYKVMPFGLKNAGATYQRAMVTLFHDMMHKEIEVYVDDMIAKSKANEDHTTILQKLF 1445

Query: 181  ---------------------GKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEK------ 240
                                 GKLLGFIVSEEGIKVD DKVKAIM++PSPETEK      
Sbjct: 1446 DRLRKYQLKLNPSKCTFGATSGKLLGFIVSEEGIKVDPDKVKAIMDMPSPETEKEVRAFL 1505

Query: 241  ---------------------------------EDCEEAFNKIKH--------------- 300
                                             EDCEEAFNKIK                
Sbjct: 1506 GRLNYISRFISHLTPTCEPIFKLLRKNNPGKWNEDCEEAFNKIKQYLQSPPVLIPPAPGR 1565

Query: 301  --------------GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCS------- 360
                          GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCC+       
Sbjct: 1566 PLILYLTVLEGSMGGVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCALVWTAHR 1625

Query: 361  ---------------------YFKK--------------FTYDIVYVTKRAIKGSAIADH 420
                                  F+K                YDIVYVTK+AIKGSA+ADH
Sbjct: 1626 LRQYMLYHTTWLISKMDPIKYIFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSALADH 1685

Query: 421  LAAQLVVDYEPISVDFPDENIFLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKV 480
            LAAQ V DYEP+SVDFPDENIFLVE DATDHETWIMLFDGASNELGHGI V LI P+GKV
Sbjct: 1686 LAAQPVADYEPMSVDFPDENIFLVENDATDHETWIMLFDGASNELGHGIGVVLISPKGKV 1745

Query: 481  FPLIAKLCFECTHNIVEYEACIMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKL 517
            FPL AKLCFECTHNI EYEACIMGLQA IDMS+KKLKVLGDSMLVIH+VKEEWETRDAKL
Sbjct: 1746 FPLTAKLCFECTHNIAEYEACIMGLQAAIDMSVKKLKVLGDSMLVIHQVKEEWETRDAKL 1805

BLAST of CSPI01G19410 vs. NCBI nr
Match: XP_031740016.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116403346 [Cucumis sativus])

HSP 1 Score: 723.8 bits (1867), Expect = 1.1e-204
Identity = 452/913 (49.51%), Postives = 479/913 (52.46%), Query Frame = 0

Query: 1    PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVP------------ 60
            PEC PVRQKLRKMK D+LIK+KE+VQKQIEA F+ VSKYPEWVANIVP            
Sbjct: 1200 PECNPVRQKLRKMKPDILIKMKEEVQKQIEAGFLIVSKYPEWVANIVPVPKKDGKVRMCV 1259

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 1260 DYRDLNRASPKDNFPLPHIDMLVDNTAGYSTFSFMDGFSGYNQIKMAEEDREKTTFITLW 1319

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 1320 GTFCYKVMPFGLKNAGATYQRAMVTLFHDMMHKEIEVYVDDMIAKSKANEDHTTILQKLF 1379

Query: 181  ---------------------GKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEK------ 240
                                 GKLLGFIVSEEGIKVD DKVKAIM++PSPETEK      
Sbjct: 1380 DRLRKYQLKLNPSKCTFGATSGKLLGFIVSEEGIKVDPDKVKAIMDMPSPETEKEVRAFL 1439

Query: 241  ---------------------------------EDCEEAFNKIKH--------------- 300
                                             EDCEEAFNKIK                
Sbjct: 1440 GRLNYISRFISHLTPTCEPIFKLLRKNNPGKWNEDCEEAFNKIKQYLQSPPVLTGPLILY 1499

Query: 301  ---------GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCS------------ 360
                     GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCC+            
Sbjct: 1500 LTVLEGSMGGVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCALVWTAHRLRQYM 1559

Query: 361  ----------------YFKK--------------FTYDIVYVTKRAIKGSAIADHLAAQL 420
                             F+K                YDIVYVTK+AIKGSA+ADHLAAQ 
Sbjct: 1560 LYHTTWLISKMDPIKYIFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSALADHLAAQP 1619

Query: 421  VVDYEPISVDFPDENIFLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKVFPLIA 480
            V DYEP+SVDFPDENIFLVE DATDHETWIMLFDGASNELGHGI V LI P+GKVFPL A
Sbjct: 1620 VADYEPMSVDFPDENIFLVENDATDHETWIMLFDGASNELGHGIGVVLISPKGKVFPLTA 1679

Query: 481  KLCFECTHNIVEYEACIMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQ 517
            KLCFECTHNI EYEACIMGLQA IDMS+KKLKVLGDSMLVIH+VKEEWETRDA +VPYSQ
Sbjct: 1680 KLCFECTHNIAEYEACIMGLQAAIDMSVKKLKVLGDSMLVIHQVKEEWETRDAXIVPYSQ 1739

BLAST of CSPI01G19410 vs. NCBI nr
Match: XP_031742240.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC116404130 [Cucumis sativus])

HSP 1 Score: 723.8 bits (1867), Expect = 1.1e-204
Identity = 453/918 (49.35%), Postives = 479/918 (52.18%), Query Frame = 0

Query: 1    PECKPVRQKLRKMKSDVLIKIKEKVQKQIEAEFITVSKYPEWVANIVP------------ 60
            PEC PVRQKLRKMK D+LIKIKE+VQKQIE  F+ VSKYPEWVANIVP            
Sbjct: 1186 PECNPVRQKLRKMKPDILIKIKEEVQKQIEVGFLIVSKYPEWVANIVPVPKKDGKVRMCV 1245

Query: 61   ------------------------------------------------------------ 120
                                                                        
Sbjct: 1246 DYRDLNRASPKDNFPLPHIDMLVDNTAGYSTFSFMDDFSGYNQIKMAEEDREKTTFITLW 1305

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 1306 GTFCYKVMPFGLKNAGATYQRAIVTLFHDMMHKEIEVYVDDMIAKSKANEDHTTILQKLF 1365

Query: 181  ---------------------GKLLGFIVSEEGIKVDLDKVKAIMEIPSPETEK------ 240
                                 GKLLGFIVSEEGIKVD DKVKAIM++PSPETEK      
Sbjct: 1366 DRLRKYQLKLNPSKCTFGATSGKLLGFIVSEEGIKVDPDKVKAIMDMPSPETEKEVRAFL 1425

Query: 241  ---------------------------------EDCEEAFNKIKH--------------- 300
                                             EDCEEAFNKIK                
Sbjct: 1426 GRLNYISRFISHLTPTCEPIFKLLRKNNPGKWNEDCEEAFNKIKQYLQSPPVLIPPAPGR 1485

Query: 301  --------------GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCS------- 360
                          GVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCC+       
Sbjct: 1486 PLILYLTVLEGSMGGVLGQHDLSGKKEHAIYYLSKKFTDYESKYSMLERTCCALVWTAHR 1545

Query: 361  ---------------------YFKK--------------FTYDIVYVTKRAIKGSAIADH 420
                                  F+K                YDIVYVTK+AIKGSA+ADH
Sbjct: 1546 LRQYMLYHTTWLISKMDPIKYIFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSALADH 1605

Query: 421  LAAQLVVDYEPISVDFPDENIFLVEKDATDHETWIMLFDGASNELGHGIRVALIFPEGKV 480
            LAAQ V DYEP+SVDFPDENIFLVE DATDHETWIMLFDGASNELGHGI V LI P+GKV
Sbjct: 1606 LAAQPVADYEPMSVDFPDENIFLVENDATDHETWIMLFDGASNELGHGIGVVLISPKGKV 1665

Query: 481  FPLIAKLCFECTHNIVEYEACIMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKL 517
            FPL AKLCFECTHNI EYEACIMGLQA IDMS+KKLKVLGDSMLVIH+VKEEWETRDAKL
Sbjct: 1666 FPLTAKLCFECTHNIAEYEACIMGLQAAIDMSVKKLKVLGDSMLVIHQVKEEWETRDAKL 1725

BLAST of CSPI01G19410 vs. TAIR 10
Match: AT3G01410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 6.7e-11
Identity = 38/123 (30.89%), Postives = 60/123 (48.78%), Query Frame = 0

Query: 195 FDGAS--NELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEACIMGLQAVIDMSLKK 254
           FDGAS  N    G    L   +  V   + +     T+N+ EY A ++GL++ +D   K 
Sbjct: 159 FDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDKGFKN 218

Query: 255 LKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFDHVHKEDNWMTDALAT 314
           + VLGDSMLV  +V+  W+T   K+    +   +L  +F+     H+ +E N   D  A 
Sbjct: 219 VHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFDIKHIAREKNSEADKQAN 278

Query: 315 LAV 316
            A+
Sbjct: 279 SAI 281

BLAST of CSPI01G19410 vs. TAIR 10
Match: AT3G01410.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 6.7e-11
Identity = 38/123 (30.89%), Postives = 60/123 (48.78%), Query Frame = 0

Query: 195 FDGAS--NELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEACIMGLQAVIDMSLKK 254
           FDGAS  N    G    L   +  V   + +     T+N+ EY A ++GL++ +D   K 
Sbjct: 159 FDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDKGFKN 218

Query: 255 LKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFDHVHKEDNWMTDALAT 314
           + VLGDSMLV  +V+  W+T   K+    +   +L  +F+     H+ +E N   D  A 
Sbjct: 219 VHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFDIKHIAREKNSEADKQAN 278

Query: 315 LAV 316
            A+
Sbjct: 279 SAI 281

BLAST of CSPI01G19410 vs. TAIR 10
Match: AT1G24090.1 (RNase H family protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-08
Identity = 48/150 (32.00%), Postives = 65/150 (43.33%), Query Frame = 0

Query: 169 ISVDFPDENIFLVEKDA-TDHETWIMLFDGAS--NELGHGIRVALIFPEGKVFPLIAKLC 228
           IS D P E +  VE  A    ET  + FDGAS  N    G    L   +G +   + +  
Sbjct: 195 ISYD-PLEKLSKVEPSAYISDETCFIEFDGASKGNPGLSGAAAVLKTEDGSLICRVRQGL 254

Query: 229 FECTHNIVEYEACIMGLQAVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVA 288
              T+N  EY A I+GL+  I+   K +KV GDS LV  ++K +W+     L    +   
Sbjct: 255 GIATNNAAEYHALILGLKYAIEKGYKNIKVKGDSKLVCMQIKGQWKVNHEVLAKLHKEAK 314

Query: 289 KLSQNFEKISFDHVHKEDNWMTDALATLAV 316
            L          HV +  N   D  A LAV
Sbjct: 315 LLCNKCVSFEISHVLRNLNADADEQANLAV 343

BLAST of CSPI01G19410 vs. TAIR 10
Match: AT5G51080.1 (RNase H family protein )

HSP 1 Score: 53.9 bits (128), Expect = 4.5e-07
Identity = 39/131 (29.77%), Postives = 58/131 (44.27%), Query Frame = 0

Query: 186 TDHETWIMLFDGAS--NELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEACIMGLQ 245
           T +ET I+ FDGAS  N    G    L   +G +   + +     T+N  EY   I+GL+
Sbjct: 181 TSYETCIIEFDGASKGNPGLSGAAAVLKTEDGSLIFKMRQGLGIATNNAAEYHGLILGLK 240

Query: 246 AVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFDHVHKED 305
             I+    K+KV  DS LV  ++K +W+     L    +   +LS         HV +  
Sbjct: 241 HAIEKGYTKIKVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQLSDKCLSFEISHVLRSL 300

Query: 306 NWMTDALATLA 315
           N   D  A +A
Sbjct: 301 NSDADEQANMA 311

BLAST of CSPI01G19410 vs. TAIR 10
Match: AT5G51080.2 (RNase H family protein )

HSP 1 Score: 53.9 bits (128), Expect = 4.5e-07
Identity = 39/131 (29.77%), Postives = 58/131 (44.27%), Query Frame = 0

Query: 186 TDHETWIMLFDGAS--NELGHGIRVALIFPEGKVFPLIAKLCFECTHNIVEYEACIMGLQ 245
           T +ET I+ FDGAS  N    G    L   +G +   + +     T+N  EY   I+GL+
Sbjct: 181 TSYETCIIEFDGASKGNPGLSGAAAVLKTEDGSLIFKMRQGLGIATNNAAEYHGLILGLK 240

Query: 246 AVIDMSLKKLKVLGDSMLVIHRVKEEWETRDAKLVPYSQYVAKLSQNFEKISFDHVHKED 305
             I+    K+KV  DS LV  ++K +W+     L    +   +LS         HV +  
Sbjct: 241 HAIEKGYTKIKVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQLSDKCLSFEISHVLRSL 300

Query: 306 NWMTDALATLA 315
           N   D  A +A
Sbjct: 301 NSDADEQANMA 311

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P274013.5e-0431.88Pro-Pol polyprotein OS=Simian foamy virus type 3 (strain LK3) OX=11644 GN=pol PE... [more]
P230744.5e-0431.88Pro-Pol polyprotein OS=Simian foamy virus type 1 OX=338478 GN=pol PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A5A7T7R43.1e-19748.93Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G00282... [more]
A0A5A7T0A14.3e-19155.79Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00070 ... [more]
A0A5D3DMD26.9e-18955.17Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold717G00160... [more]
A0A5D3BAL89.3e-17851.88Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10G00460 ... [more]
A0A5A7UKG21.5e-17255.66Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G00105... [more]
Match NameE-valueIdentityDescription
XP_031739260.11.3e-20549.73LOW QUALITY PROTEIN: uncharacterized protein LOC116402917 [Cucumis sativus][more]
XP_031741698.13.7e-20549.46uncharacterized protein LOC116403895 [Cucumis sativus][more]
XP_031737561.14.9e-20549.51LOW QUALITY PROTEIN: uncharacterized protein LOC116402452 [Cucumis sativus][more]
XP_031740016.11.1e-20449.51LOW QUALITY PROTEIN: uncharacterized protein LOC116403346 [Cucumis sativus][more]
XP_031742240.11.1e-20449.35LOW QUALITY PROTEIN: uncharacterized protein LOC116404130 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G01410.16.7e-1130.89Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G01410.26.7e-1130.89Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G24090.11.4e-0832.00RNase H family protein [more]
AT5G51080.14.5e-0729.77RNase H family protein [more]
AT5G51080.24.5e-0729.77RNase H family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 420..516
e-value: 2.7E-19
score: 71.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 192..322
e-value: 1.5E-25
score: 91.7
NoneNo IPR availableGENE3D3.10.10.10HIV Type 1 Reverse Transcriptase, subunit A, domain 1coord: 1..69
e-value: 2.3E-5
score: 26.4
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 1..48
NoneNo IPR availablePANTHERPTHR24559:SF322RNA-DIRECTED DNA POLYMERASE (REVERSE TRANSCRIPTASE), RIBONUCLEASE H-LIKE PROTEINcoord: 139..329
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 139..329
NoneNo IPR availablePANTHERPTHR24559:SF322RNA-DIRECTED DNA POLYMERASE (REVERSE TRANSCRIPTASE), RIBONUCLEASE H-LIKE PROTEINcoord: 1..48
NoneNo IPR availableCDDcd09279RNase_HI_likecoord: 191..314
e-value: 1.06888E-33
score: 122.196
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 195..314
e-value: 1.8E-15
score: 56.9
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 187..316
score: 10.418979
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 397..499
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 189..311
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 1..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G19410.1CSPI01G19410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity