CSPI01G18500 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G18500
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytochrome P450
LocationChr1: 13941582 .. 13944142 (+)
RNA-Seq ExpressionCSPI01G18500
SyntenyCSPI01G18500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACATTTTAAGTAAAATTATAGATGTATTACCCATTTCTCAAGAGAGAAAAAATAATAATAATGAAGGAGGTGAAGATTGAATCTCCGGCATGGTCCGCCACGGTGGTGACGATTGTGGTGTTGTTGATCTTCATTATAACTGCGAAAGTTGGTTTGGTTTTATGGTGGAAACCAAAAAAAATAGAAAAACACTTTGATAGACAAGGAATTAAAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTCAAAGAACTCGTTTCTTTGATGCTCGCTGCTTCTTCCCATCCAATGCCCAATTTCTCTCATAATATTCTCCCTCGAGTTCTCTCTTTTTATCATCATTGGAAGAAAATTTACGGTACGCTTTTATTATTATTATTATTATTTTTTTTTTTTTTTATTAATTGTACCATATTATTCCACTCCCCACCGGAGGATTTTCTCTGATTCAACGAAAATGTCTGTTTGGATTGAGTTTCTAGGTTCCACTTTTCTAATTTGGTTTGGACCTATTGTTCGAGTCGCTGTCTCTGACCCAGACATGATCCGAGAAATCTTCGCCACAAATTCCCACTTCTACGAGAAAAACGAAGCTCATCCTCTTGTTAAACAGCTTGAAGGTGATGGTCTCCTCACTCTCACTGGCCAAAAATGGGCTCTCCATCGCAAAATCATATCTCCCACTTTCCATGTGGATAATCTGAAAGTAAGGTTTTAGTTATATTATTATTGGTTTAATAATGTATATATGTTTTAAGCAGTTGATGGAGTTATATATATGTGTGAGTTTTGGAATTGTGTGTGTGTGTGTGGGTATAGTTGTTGATTCCGTTGGTGGAAGAGAGTGTGGTGGAAATGGTGGAGAAATGGTGGTCGATGGCGGAGAGTATGGACTCGGGTGAAGTGGAGATCGATGTTTACGATTGGTTCCAAACCCTAACGGAAGATGTTATTACTAAAACAGCATTTGGAAGTAGCTACGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCACAAGCTTTTCAAAAGGTCTTCATTCCTGGCTATAGGTAATTAATAATTTATTTATTTTATTTTATTATTTGAAAGAAATTCCTTTTTGGGATTTAATTAATTTAATTTTCACAGATTTCTACCGACAAGAACGAATATAAATTCATGGAAATTAGACAGGGAAATAAAGAAATCATTGATGAAATTAATCGAGGGACGAAAAAGGAATTTAGAGAAAAAGAATTGGGGGATGAATGAAAAAAGACCAAAAGATTTGTTAGGGTTGATGATTCAAGCTTCTTTGTTAAACAACAACAACAATAATAATTCCTCCATTACTGTCCACGACATCGTTGAAGAGTGCAAAAGCTTCTTCTTTGCTGGCAAACAAACTACTTCCAATTTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCACAGTGGCAAATCCAGGCACGTGACGAGGTCTTTAACGTCTGCGGTCCACGTGCCCTCCCTTCTAAACATGACGTCTCTAACTTCAAAATCGTAAGCCCTTTCTCTTTATCCCTCTCTTTTCCTTTTTCCATTTCATCTTCTTCCTTTTTTATATAAAAAAAAAAATAAATAAATATATATATAAGATTAAAATACCATTTTATCCCTCATACTTTGACTTAGATAACAATAATTATATGTATCCATTAAAATAATAGCTCTACCATAATAATAATAACTGATTTGTTTAAGCAATACATTATTATTGAGTATATAGGGTAAAAAAAGAAGTTAAAAAAATTATTAGTTTTGTCATTTTACTAATAAAAACTAATAAACTCCCAACTTTTGGTTTAGGTTGCTTGGTTTATTTTGATGGATTTCTTTTCTTTATTTTTCTGATTGGGTGGGGTAAATGATTGGGTGTGGCAGCTAAGCATGATACTAAATGAATCCTTACGATTATACCCACCCACGGTGGCAACTATCCGACGTGCAAAGACTGACGTGGAGCTAGCGGGACGAATGATCCCACGCGGGACCGAGTTTTTGATCCCAATCATAGCCGTTCATCACGATCAAACCATATGGGGGAACGACGCAAGCGAGTTCAACCCAGGGCGGTTCTCTGAAGGAGTGAGCAAAGCCGCAAATCACCCCGCCGGGTTCATTCCATTTGGACTCGGTGTCCGTACATGCATTGGTCAAAATCTAGCCCTTTTGGAAGCAAAATTAACCCTCGCAATTATACTCCAACGCTTCACATTTCGTTTGGCTCCCACTTATCAACATGCACCCACGGTCCTGATGCTTCTTTATCCACAGTATGGGGCCCCCATTATTTTTCAGAAGATACCCATAAATTAAGATACCTATATATATATATATATGGCTAATTTAACCCCATAATTATTATTAATCTCGATTAGTATAGTTAGATCATTACCAAATGGCTAACACTATAGGGTAGTGTCTTCAAATATATATCATTCTCTCTAAGGGGGAGATTTCTGTATTCAATCGGTGTTTAAGTTTTAATCTGTTTCTATACTTTACTTTTAGAG

mRNA sequence

CAACATTTTAAGTAAAATTATAGATGTATTACCCATTTCTCAAGAGAGAAAAAATAATAATAATGAAGGAGGTGAAGATTGAATCTCCGGCATGGTCCGCCACGGTGGTGACGATTGTGGTGTTGTTGATCTTCATTATAACTGCGAAAGTTGGTTTGGTTTTATGGTGGAAACCAAAAAAAATAGAAAAACACTTTGATAGACAAGGAATTAAAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTCAAAGAACTCGTTTCTTTGATGCTCGCTGCTTCTTCCCATCCAATGCCCAATTTCTCTCATAATATTCTCCCTCGAGTTCTCTCTTTTTATCATCATTGGAAGAAAATTTACGGTTCCACTTTTCTAATTTGGTTTGGACCTATTGTTCGAGTCGCTGTCTCTGACCCAGACATGATCCGAGAAATCTTCGCCACAAATTCCCACTTCTACGAGAAAAACGAAGCTCATCCTCTTGTTAAACAGCTTGAAGGTGATGGTCTCCTCACTCTCACTGGCCAAAAATGGGCTCTCCATCGCAAAATCATATCTCCCACTTTCCATGTGGATAATCTGAAATTGTTGATTCCGTTGGTGGAAGAGAGTGTGGTGGAAATGGTGGAGAAATGGTGGTCGATGGCGGAGAGTATGGACTCGGGTGAAGTGGAGATCGATGTTTACGATTGGTTCCAAACCCTAACGGAAGATGTTATTACTAAAACAGCATTTGGAAGTAGCTACGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCACAAGCTTTTCAAAAGGTCTTCATTCCTGGCTATAGATTTCTACCGACAAGAACGAATATAAATTCATGGAAATTAGACAGGGAAATAAAGAAATCATTGATGAAATTAATCGAGGGACGAAAAAGGAATTTAGAGAAAAAGAATTGGGGGATGAATGAAAAAAGACCAAAAGATTTGTTAGGGTTGATGATTCAAGCTTCTTTGTTAAACAACAACAACAATAATAATTCCTCCATTACTGTCCACGACATCGTTGAAGAGTGCAAAAGCTTCTTCTTTGCTGGCAAACAAACTACTTCCAATTTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCACAGTGGCAAATCCAGGCACGTGACGAGGTCTTTAACGTCTGCGGTCCACGTGCCCTCCCTTCTAAACATGACGTCTCTAACTTCAAAATCCTAAGCATGATACTAAATGAATCCTTACGATTATACCCACCCACGGTGGCAACTATCCGACGTGCAAAGACTGACGTGGAGCTAGCGGGACGAATGATCCCACGCGGGACCGAGTTTTTGATCCCAATCATAGCCGTTCATCACGATCAAACCATATGGGGGAACGACGCAAGCGAGTTCAACCCAGGGCGGTTCTCTGAAGGAGTGAGCAAAGCCGCAAATCACCCCGCCGGGTTCATTCCATTTGGACTCGGTGTCCGTACATGCATTGGTCAAAATCTAGCCCTTTTGGAAGCAAAATTAACCCTCGCAATTATACTCCAACGCTTCACATTTCGTTTGGCTCCCACTTATCAACATGCACCCACGGTCCTGATGCTTCTTTATCCACAGTATGGGGCCCCCATTATTTTTCAGAAGATACCCATAAATTAAGATACCTATATATATATATATATGGCTAATTTAACCCCATAATTATTATTAATCTCGATTAGTATAGTTAGATCATTACCAAATGGCTAACACTATAGGGTAGTGTCTTCAAATATATATCATTCTCTCTAAGGGGGAGATTTCTGTATTCAATCGGTGTTTAAGTTTTAATCTGTTTCTATACTTTACTTTTAGAG

Coding sequence (CDS)

ATGTATTACCCATTTCTCAAGAGAGAAAAAATAATAATAATGAAGGAGGTGAAGATTGAATCTCCGGCATGGTCCGCCACGGTGGTGACGATTGTGGTGTTGTTGATCTTCATTATAACTGCGAAAGTTGGTTTGGTTTTATGGTGGAAACCAAAAAAAATAGAAAAACACTTTGATAGACAAGGAATTAAAGGCCCTCCTTATCGTTTCTTCATTGGTAATTTCAAAGAACTCGTTTCTTTGATGCTCGCTGCTTCTTCCCATCCAATGCCCAATTTCTCTCATAATATTCTCCCTCGAGTTCTCTCTTTTTATCATCATTGGAAGAAAATTTACGGTTCCACTTTTCTAATTTGGTTTGGACCTATTGTTCGAGTCGCTGTCTCTGACCCAGACATGATCCGAGAAATCTTCGCCACAAATTCCCACTTCTACGAGAAAAACGAAGCTCATCCTCTTGTTAAACAGCTTGAAGGTGATGGTCTCCTCACTCTCACTGGCCAAAAATGGGCTCTCCATCGCAAAATCATATCTCCCACTTTCCATGTGGATAATCTGAAATTGTTGATTCCGTTGGTGGAAGAGAGTGTGGTGGAAATGGTGGAGAAATGGTGGTCGATGGCGGAGAGTATGGACTCGGGTGAAGTGGAGATCGATGTTTACGATTGGTTCCAAACCCTAACGGAAGATGTTATTACTAAAACAGCATTTGGAAGTAGCTACGAAGATGGTAAAGCTATATTTAAGCTTCAAACTCAACAAATGATATTAGCTTCACAAGCTTTTCAAAAGGTCTTCATTCCTGGCTATAGATTTCTACCGACAAGAACGAATATAAATTCATGGAAATTAGACAGGGAAATAAAGAAATCATTGATGAAATTAATCGAGGGACGAAAAAGGAATTTAGAGAAAAAGAATTGGGGGATGAATGAAAAAAGACCAAAAGATTTGTTAGGGTTGATGATTCAAGCTTCTTTGTTAAACAACAACAACAATAATAATTCCTCCATTACTGTCCACGACATCGTTGAAGAGTGCAAAAGCTTCTTCTTTGCTGGCAAACAAACTACTTCCAATTTGCTGACGTGGACTACTGTCTTGCTGGCAATGCATCCACAGTGGCAAATCCAGGCACGTGACGAGGTCTTTAACGTCTGCGGTCCACGTGCCCTCCCTTCTAAACATGACGTCTCTAACTTCAAAATCCTAAGCATGATACTAAATGAATCCTTACGATTATACCCACCCACGGTGGCAACTATCCGACGTGCAAAGACTGACGTGGAGCTAGCGGGACGAATGATCCCACGCGGGACCGAGTTTTTGATCCCAATCATAGCCGTTCATCACGATCAAACCATATGGGGGAACGACGCAAGCGAGTTCAACCCAGGGCGGTTCTCTGAAGGAGTGAGCAAAGCCGCAAATCACCCCGCCGGGTTCATTCCATTTGGACTCGGTGTCCGTACATGCATTGGTCAAAATCTAGCCCTTTTGGAAGCAAAATTAACCCTCGCAATTATACTCCAACGCTTCACATTTCGTTTGGCTCCCACTTATCAACATGCACCCACGGTCCTGATGCTTCTTTATCCACAGTATGGGGCCCCCATTATTTTTCAGAAGATACCCATAAATTAA

Protein sequence

MYYPFLKREKIIIMKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN*
Homology
BLAST of CSPI01G18500 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.1e-215
Identity = 361/526 (68.63%), Postives = 437/526 (83.08%), Query Frame = 0

Query: 20  ESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELV 79
           ES +W    V ++ +++ ++  K   +LWW+P+KIE+HF +QGI+GPPY FFIGN KELV
Sbjct: 4   ESSSWFIPKVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELV 63

Query: 80  SLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFA 139
            +ML ASSHPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+IREIF 
Sbjct: 64  GMMLKASSHPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF- 123

Query: 140 TNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVE 199
           + S FYEKNEAHPLVKQLEGDGLL+L G+KWA HRKIISPTFH++NLKLL+P+V +SV +
Sbjct: 124 SKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTD 183

Query: 200 MVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILAS 259
           MV+KW    +  ++GEVE+DVY+WFQ LTEDVI++TAFGSSYEDG+A+F+LQ QQM+L +
Sbjct: 184 MVDKW--SDKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCA 243

Query: 260 QAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRN-LEKKNWGMNEKRPKDL 319
           +AFQKVFIPGYRF PTR N+ SWKLD+EI+KSL+KLIE R++N ++ +     E   KDL
Sbjct: 244 EAFQKVFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDL 303

Query: 320 LGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQ 379
           LGLMIQA           ++TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ +
Sbjct: 304 LGLMIQA----------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAK 363

Query: 380 ARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRMIPR 439
           ARDEV  VCG R +P+K  V   K LSMILNESLRLYPP VATIRRAK+DV+L G  IP 
Sbjct: 364 ARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPC 423

Query: 440 GTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQNLA 499
           GTE LIPIIAVHHDQ IWGND +EFNP RF++GV +AA HP GFIPFGLGVRTCIGQNLA
Sbjct: 424 GTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLA 483

Query: 500 LLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 545
           +L+AKLTLA+++QRFTF LAPTYQHAPTVLMLLYPQ+GAPI F+++
Sbjct: 484 ILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

BLAST of CSPI01G18500 vs. ExPASy Swiss-Prot
Match: B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 2.6e-188
Identity = 319/514 (62.06%), Postives = 406/514 (78.99%), Query Frame = 0

Query: 39  ITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELVSLMLAASSHPM--PNFSHN 98
           +  KV  VLWW+P+++E+HF RQGI GP YRF +G  +E+V+LM+AAS+ PM  P  SHN
Sbjct: 17  VAVKVLEVLWWRPRRVEEHFARQGITGPRYRFLVGCVREMVALMVAASAKPMPPPYRSHN 76

Query: 99  ILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFATNSHFYEKNEAHPLVKQ 158
           +LPRVL+FYHHWKKIYGSTFLIWFGP  R+A++DP++IRE+    +  +++ E+HP+V+Q
Sbjct: 77  VLPRVLAFYHHWKKIYGSTFLIWFGPTPRLAIADPELIREVLLARADRFDRYESHPMVRQ 136

Query: 159 LEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVEMVEKWWSMAESMDSGEV 218
           LEG+GL++L G KWA HR++++P FH+DNL+LL+P V  +V++M +KW +MAE+  SGEV
Sbjct: 137 LEGEGLVSLRGDKWAHHRRVLTPAFHMDNLRLLLPCVGMTVLDMADKWRAMAEADKSGEV 196

Query: 219 EIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFIPGYRFLPTR 278
           EIDV DWFQ +TED IT+TAFG SYEDGK +FKLQ Q M  AS+AF+KVFIPGYRFLPT+
Sbjct: 197 EIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRKVFIPGYRFLPTK 256

Query: 279 TNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKR---PKDLLGLMIQASLLNNNNN 338
            N +SWKLD+EI+K+L+ LI GR++       G +EK     KDLLGLMI A+  +N   
Sbjct: 257 KNTSSWKLDKEIRKNLVTLI-GRRQEA-----GDDEKLDGCAKDLLGLMINAAASSNGGK 316

Query: 339 NN----SSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGP 398
            +    S ITV+DIVEECK+FFFAGKQTTSNLLTW  V+LAMHP+WQ +AR EV +VCG 
Sbjct: 317 RSALPVSPITVNDIVEECKTFFFAGKQTTSNLLTWAIVVLAMHPEWQERARQEVLDVCGA 376

Query: 399 RALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRM-IPRGTEFLIPIIA 458
             +PS+  ++  K L MILNE+LRLYPP VAT+RRAK DVEL G + IPR TE LIPI+A
Sbjct: 377 DGVPSREQLAKLKTLGMILNETLRLYPPAVATVRRAKADVELGGYLRIPRDTELLIPIMA 436

Query: 459 VHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQNLALLEAKLTLAI 518
           VHHD  +WG DA++FNP RF+ GV++AA HPA FIPFGLG R CIGQNLA+LEAKLT+A+
Sbjct: 437 VHHDARLWGPDAAQFNPARFAGGVARAARHPAAFIPFGLGARMCIGQNLAILEAKLTVAV 496

Query: 519 ILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQ 543
           IL RF FRL+  Y HAPTVLMLL+PQYGAPI+F+
Sbjct: 497 ILHRFEFRLSARYVHAPTVLMLLHPQYGAPIVFR 524

BLAST of CSPI01G18500 vs. ExPASy Swiss-Prot
Match: Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 7.9e-177
Identity = 301/530 (56.79%), Postives = 392/530 (73.96%), Query Frame = 0

Query: 25  SATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELVSLMLA 84
           +A      V +   + A++   LWW+P+++E HF  QG++GPPYRF +G+ +E+V+LM  
Sbjct: 19  AAVAAAAAVWVTMHVAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAE 78

Query: 85  ASSHPM-PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFATNSH 144
           ASS PM P  SHN LPRVL+FYH+W+KIYG  FLIWFGP  R+ V++P++IREIF T + 
Sbjct: 79  ASSKPMSPPTSHNALPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRAD 138

Query: 145 FYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVEMVEK 204
            +++ EAHP+V+QLEGDGL++L G KWALHR++++  F+ DNL  LIP V +SV  +  K
Sbjct: 139 AFDRYEAHPVVRQLEGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAK 198

Query: 205 WWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQ 264
           W +MAE+  SGEVE+DV +WFQ +TE+ IT+  FG SY+DG+ +F +Q Q M  AS+AF+
Sbjct: 199 WGAMAEAGGSGEVEVDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFR 258

Query: 265 KVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRPKDLLGLMI 324
           KV +PGYRFLPT+ N  SW+LDREI++SLM+LI GR+ +  ++    ++   +DLLGLMI
Sbjct: 259 KVLVPGYRFLPTKKNRLSWRLDREIRRSLMRLI-GRRSDEAEQGEKADDGSFRDLLGLMI 318

Query: 325 QASLL--------NNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW 384
            A              N+  ++I V D++EECK+FFFAGKQTT+NLLTW TVLLAMHP W
Sbjct: 319 NAGAAAATRGNAGGEKNSPAAAIPVEDMLEECKTFFFAGKQTTTNLLTWATVLLAMHPDW 378

Query: 385 QIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELA-GR 444
           Q +AR EVF+VCG   LPSK  +   K L MI+NE+LRLYPP VATIRRAK DV+L+ G 
Sbjct: 379 QERARREVFDVCGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQLSDGC 438

Query: 445 MIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIG 504
           MIPR  E L+PI+A+HHD   WG DAS+FNP RF+ G SKAA HP  FIPFGLG R C+G
Sbjct: 439 MIPRDMELLVPIMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGSRMCVG 498

Query: 505 QNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 545
           QNLA LEAKLT+AI+LQRF  R +P Y HAPTVLMLLYPQYGAP+IF+ +
Sbjct: 499 QNLARLEAKLTMAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPL 547

BLAST of CSPI01G18500 vs. ExPASy Swiss-Prot
Match: Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.5e-167
Identity = 292/540 (54.07%), Postives = 384/540 (71.11%), Query Frame = 0

Query: 26  ATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELVSLMLAA 85
           A  V   VLL+  + A+V   +WW+P+++E HF  QG++GPPYRF +G  +E+V+LM  A
Sbjct: 4   AVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGPPYRFLVGCVREMVALMAEA 63

Query: 86  SSHPMPNFS-HNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFATNSHF 145
           ++ PMP  + HN LPRVL+FYH+W+KIYG TFLIWFGP  R+ V++P+M+REIF T +  
Sbjct: 64  TAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPTPRLTVAEPEMVREIFLTRAEA 123

Query: 146 YEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVEMVEKW 205
           +++ EAHP+V+QLEGDGL++L G KWA HR++++P F+ DNL  L+P V  SV  + E+W
Sbjct: 124 FDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGFYPDNLNRLVPHVGRSVAALAERW 183

Query: 206 WSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQK 265
            +MA     GEVE+DV +WFQ + E+ IT+  FG SY+ G+ +F+LQ + M  AS+AF+K
Sbjct: 184 RAMA-CAGGGEVEVDVAEWFQAVAEEAITRATFGRSYDSGRVVFRLQARLMAFASEAFRK 243

Query: 266 VFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRN------------LEKKNWGMNE 325
           V +PGYRFLPT+ N  SW LDREI++ L++LI  R                +K++ G N 
Sbjct: 244 VLVPGYRFLPTKKNRMSWGLDREIRRGLVRLIGRRSGGDGGEEDETTTELKDKQDSGFN- 303

Query: 326 KRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH 385
               DLLGLMI A +       + ++ V D+VEECK+FFFAGKQTT+NLLTW TVLLAMH
Sbjct: 304 ----DLLGLMINAGV-------DRTMPVEDMVEECKTFFFAGKQTTTNLLTWATVLLAMH 363

Query: 386 PQWQIQARDEVFNVCGPRA--LPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVE 445
           P WQ +AR EV  VCG  A  LP+K  +   K L MILNE+LRLYPP VATIRRAK DV 
Sbjct: 364 PDWQDRARREVLAVCGDAAGELPTKDHLPKLKTLGMILNETLRLYPPAVATIRRAKFDVT 423

Query: 446 LAGR--------MIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGF 505
           L G          IPR TE L+PI+A+HHD  +WG DA++FNP RF+ G ++AA HP  F
Sbjct: 424 LGGGGDGDAGGIHIPRDTELLVPIMAIHHDARLWGPDAAQFNPARFASGAARAAKHPLAF 483

Query: 506 IPFGLGVRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQ 543
           IPFGLG R CIGQ+LA+LEAKLT+A++LQRF   L+PTY HAPTVLMLL+PQYGAP+IF+
Sbjct: 484 IPFGLGSRMCIGQSLAILEAKLTMAVLLQRFDLALSPTYVHAPTVLMLLHPQYGAPLIFR 530

BLAST of CSPI01G18500 vs. ExPASy Swiss-Prot
Match: Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-146
Identity = 256/535 (47.85%), Postives = 354/535 (66.17%), Query Frame = 0

Query: 23  AWSATVVTIVV-----LLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKE 82
           +WS      V      + ++   A+    LWW+P+++E+HF  QG++GP YRFF+G+  E
Sbjct: 10  SWSGAAAVAVAAAAAWVAVYAAAARAAEALWWRPRRVERHFAAQGVRGPGYRFFVGSSIE 69

Query: 83  LVSLMLAASSHPM-PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRE 142
           LV LM+ A+S PM P  SH+ILPRVL FYHHW+K+YG   LIWFG   R+ VS+P++IRE
Sbjct: 70  LVRLMVDAASRPMEPPTSHDILPRVLPFYHHWRKLYGPMHLIWFGRTPRLVVSEPELIRE 129

Query: 143 IFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEES 202
           +  T +  +++ EAHP++ Q EG GL  L G++WA  R++++P FH +NL+++ P V  +
Sbjct: 130 VLLTRADHFDRYEAHPMICQFEGYGLSNLHGERWARRRRVLTPAFHTENLRMIAPFVAGT 189

Query: 203 VVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFG-SSYEDGKAIFKLQTQQM 262
           V  M+++    A +  +GE E+DV +WFQ + ++ IT  AFG  +Y+DG A+F+LQ +  
Sbjct: 190 VTRMLDELAERARAGGAGEAEVDVAEWFQRVPQEAITFAAFGRRNYDDGAAVFRLQDELA 249

Query: 263 ILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEG-----RKRNLEKKNWGM 322
             A++A  KV+IPGYRFLPTR N   W+LDREI+  L K + G          +  + G 
Sbjct: 250 GYATEAHSKVYIPGYRFLPTRKNRRVWQLDREIRSHLAKFVTGLQSCSSSHGDDADDGGD 309

Query: 323 NEKRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLA 382
                ++ +  M  A            +T  +I+EE K+FFFAGK+T SNLLTWTTV LA
Sbjct: 310 GGGGMREFMSFMAPA------------MTAGEIIEESKNFFFAGKETLSNLLTWTTVALA 369

Query: 383 MHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVE 442
           MHP+WQ +AR EV  VCG   LP+K  +   K L MILNE+LRLYPP VA IR AK DVE
Sbjct: 370 MHPEWQERARREVVAVCGRGDLPTKDHLPKLKTLGMILNETLRLYPPAVAMIRTAKEDVE 429

Query: 443 LAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRF-SEGVSKAANHPAGFIPFGLGV 502
           L G ++P GTE +IPI+AVHHD   WG+DA+EFNP RF ++       HP  F+PFG G 
Sbjct: 430 LGGCVVPAGTEVMIPIMAVHHDAAAWGDDAAEFNPARFAADDDGGRRRHPMAFMPFGGGA 489

Query: 503 RTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 545
           R CIGQN+AL+EAK+ LA++L+RF FRL+P Y HAP VLM+L PQ+GAP+IF+ +
Sbjct: 490 RVCIGQNMALMEAKVALAVVLRRFEFRLSPAYVHAPRVLMILSPQFGAPVIFRPL 532

BLAST of CSPI01G18500 vs. ExPASy TrEMBL
Match: A0A0A0LU20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0

Query: 14  MKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG 73
           MKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG
Sbjct: 1   MKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG 60

Query: 74  NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 133
           NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM
Sbjct: 61  NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120

Query: 134 IREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV 193
           IREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV
Sbjct: 121 IREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV 180

Query: 194 EESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 253
           EESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ
Sbjct: 181 EESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 240

Query: 254 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEK 313
           QMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEK
Sbjct: 241 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEK 300

Query: 314 RPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP 373
           RPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP
Sbjct: 301 RPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP 360

Query: 374 QWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAG 433
           QWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAG
Sbjct: 361 QWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAG 420

Query: 434 RMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCI 493
           RMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCI
Sbjct: 421 RMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCI 480

Query: 494 GQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 548
           GQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN
Sbjct: 481 GQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 534

BLAST of CSPI01G18500 vs. ExPASy TrEMBL
Match: A0A1S3CMW0 (cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 1.0e-299
Identity = 513/537 (95.53%), Postives = 521/537 (97.02%), Query Frame = 0

Query: 14  MKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG 73
           MKEVKIESPAWSA  VT  VLLIFII+AKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG
Sbjct: 1   MKEVKIESPAWSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG 60

Query: 74  NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 133
           NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM
Sbjct: 61  NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120

Query: 134 IREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV 193
           IREIFATNS +YEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV
Sbjct: 121 IREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV 180

Query: 194 EESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 253
           EESVVEMVEKWWSMAES++S EVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQ Q
Sbjct: 181 EESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQNQ 240

Query: 254 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEK 313
           QMILASQAFQKVFIPGYRFLPTRTNINSWKLD+EIKKSLMKLIEGRK NLEKKNWGMNEK
Sbjct: 241 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKGNLEKKNWGMNEK 300

Query: 314 RPKDLLGLMIQASLL-----NNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVL 373
           RPKDLLGLMIQASLL     NNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVL
Sbjct: 301 RPKDLLGLMIQASLLNNNNNNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVL 360

Query: 374 LAMHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTD 433
           LAMHPQWQIQARDEV NVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAK D
Sbjct: 361 LAMHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKAD 420

Query: 434 VELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLG 493
           VELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVS+A NHPAGFIPFGLG
Sbjct: 421 VELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGLG 480

Query: 494 VRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 546
           VRTCIGQNLA+LEAKLT+AIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP
Sbjct: 481 VRTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 537

BLAST of CSPI01G18500 vs. ExPASy TrEMBL
Match: A0A6J1H9G1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.2e-252
Identity = 434/532 (81.58%), Postives = 469/532 (88.16%), Query Frame = 0

Query: 16  EVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNF 75
           E ++ES       V    +++ I+ AK+GLVLWWKP++IE HF RQGI GPPYRFFIGNF
Sbjct: 2   EEELESAVEVPFGVAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNF 61

Query: 76  KELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR 135
           KELVS+M AASSHP+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIR
Sbjct: 62  KELVSMMAAASSHPIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIR 121

Query: 136 EIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEE 195
           EIF TNSHFYEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH DNLKLLIPLVEE
Sbjct: 122 EIFITNSHFYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEE 181

Query: 196 SVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 255
           SV++MV+KW SM E   SGEVEIDV+D FQTLTEDVITKTAFG SYEDGKAIFK+QTQQM
Sbjct: 182 SVMDMVQKWSSMTEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQM 241

Query: 256 ILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRP 315
           ILAS+AFQKVFIPGYRFLPTRTN+ SWKL++EIKKSL+KLIEGRKRN E KNW +NEK P
Sbjct: 242 ILASEAFQKVFIPGYRFLPTRTNMKSWKLEKEIKKSLLKLIEGRKRNFE-KNWAINEKGP 301

Query: 316 KDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW 375
           KDLLGLMIQA           SI+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+W
Sbjct: 302 KDLLGLMIQA-----------SISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRW 361

Query: 376 QIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRM 435
           QI AR EVF +CGPRALPS  DVS FKILSMILNESLRLYPPTVATIRRA  DVEL GRM
Sbjct: 362 QILARHEVFTICGPRALPSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVELGGRM 421

Query: 436 IPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQ 495
           IPRGTEFLIPI+AVHHDQTIWGNDASEFNP RFS+GVS+A NHP GFIPFGLGVR CIGQ
Sbjct: 422 IPRGTEFLIPILAVHHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQ 481

Query: 496 NLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 548
           NLA+LEAKL + IILQR +FRLAPTYQHAPTVLMLLYPQYGAPI+F+KI +N
Sbjct: 482 NLAVLEAKLAMVIILQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIALN 521

BLAST of CSPI01G18500 vs. ExPASy TrEMBL
Match: A0A6J1JHK6 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111485166 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.3e-250
Identity = 431/532 (81.02%), Postives = 464/532 (87.22%), Query Frame = 0

Query: 16  EVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNF 75
           E  +E P +      +V   + I+ AK+GLVLWWKP+++E HF RQGI GP YRFFIGNF
Sbjct: 6   ESAVEVP-FGVAAAAVVAAAVVILGAKMGLVLWWKPRRMENHFLRQGIGGPRYRFFIGNF 65

Query: 76  KELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR 135
           KELVS+M AASSHPMP FSHNILPRVLSFYHHWKKIYGSTFLIWFGPI RVAVSDPDMIR
Sbjct: 66  KELVSMMAAASSHPMPKFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIARVAVSDPDMIR 125

Query: 136 EIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEE 195
           EIF TNSH YEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH DN+KLLIPL+EE
Sbjct: 126 EIFITNSHLYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHSDNIKLLIPLIEE 185

Query: 196 SVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 255
            +VEMV+KW SMAE    G VEIDV+D FQTLTEDVITKTAFG SYEDGKAIFKLQTQQM
Sbjct: 186 IIVEMVQKWSSMAEETTLGGVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKLQTQQM 245

Query: 256 ILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRP 315
           ILAS+AFQKVFIPGYRFLPTRTN+ SWKL++EIKKSL+KLIEGRKRN E KNW +NEK P
Sbjct: 246 ILASEAFQKVFIPGYRFLPTRTNMKSWKLEKEIKKSLIKLIEGRKRNFE-KNWAINEKGP 305

Query: 316 KDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW 375
           KDLLGLMIQA           SI+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW
Sbjct: 306 KDLLGLMIQA-----------SISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW 365

Query: 376 QIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRM 435
           QI AR EVF +CGPRALPS  DVS FKILSMILNESLRLYPPTVATIRRA  D+EL GRM
Sbjct: 366 QILARHEVFTICGPRALPSPDDVSKFKILSMILNESLRLYPPTVATIRRANADMELGGRM 425

Query: 436 IPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQ 495
           IPRGTEFLIPI+AVHHDQTIWGNDASEFNP RFS+GVS+A NHP GFIPFGLGVR CIGQ
Sbjct: 426 IPRGTEFLIPILAVHHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQ 485

Query: 496 NLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 548
           NLA+LEAKL + IILQR +FRLAPTYQHAPTVLMLLYPQYGAPI+F+KIP+N
Sbjct: 486 NLAILEAKLAMVIILQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIPLN 524

BLAST of CSPI01G18500 vs. ExPASy TrEMBL
Match: A0A6J1EZI1 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440975 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.7e-246
Identity = 420/524 (80.15%), Postives = 471/524 (89.89%), Query Frame = 0

Query: 22  PAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELVSL 81
           P   +  +  +  L+FI+  K+G  LWWKP++IE HF RQGI+GPPYRFFIGNFKEL S+
Sbjct: 7   PPLDSIKIPFLCALLFILAVKMGAALWWKPRRIEAHFLRQGIRGPPYRFFIGNFKELDSM 66

Query: 82  MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFATN 141
           MLAA+S PMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR+I  TN
Sbjct: 67  MLAATSRPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIRDILTTN 126

Query: 142 SHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVEMV 201
           S FYEKNEAHPL+K+LEGDGLLTL  QKWALHRKIISP F+++NLKLLIP+VEESVV+MV
Sbjct: 127 SEFYEKNEAHPLIKKLEGDGLLTLKDQKWALHRKIISPVFNMENLKLLIPVVEESVVDMV 186

Query: 202 EKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQA 261
           EKW +MA    S EVEIDV+DWFQTL EDV+TKT FGSSYEDGKAIFKLQTQQMILAS+A
Sbjct: 187 EKWSAMAGGGASDEVEIDVFDWFQTLAEDVVTKTVFGSSYEDGKAIFKLQTQQMILASEA 246

Query: 262 FQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRPKDLLGL 321
           FQKVFIPGYRFLPTRTNINSWKLD+EI+KSLMKLIEGRK N  +K W M+EK PKDLLGL
Sbjct: 247 FQKVFIPGYRFLPTRTNINSWKLDKEIRKSLMKLIEGRKGN-SQKIWAMDEKAPKDLLGL 306

Query: 322 MIQASLLNNNNNN-NSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 381
           MIQAS  ++++++ +++I+VHDIVEECK+FFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR
Sbjct: 307 MIQASNSSSSSSSLHTNISVHDIVEECKTFFFAGKQTTSNLLTWTTVLLAMHPQWQIQAR 366

Query: 382 DEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRMIPRGT 441
           DEV +VCGPRALPS  D+S FKILSMILNESLRLYPPTVATIRRAKT VEL GRMIPRGT
Sbjct: 367 DEVLSVCGPRALPSTDDLSKFKILSMILNESLRLYPPTVATIRRAKTVVELRGRMIPRGT 426

Query: 442 EFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQNLALL 501
           EFLIPI+AVHHDQ IWGNDASEFNPGRFSEGV  A NHPAGFIPFG G+R CIG+NLALL
Sbjct: 427 EFLIPILAVHHDQNIWGNDASEFNPGRFSEGVGHAGNHPAGFIPFGHGLRACIGKNLALL 486

Query: 502 EAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 545
           +AKL +AII+QRF+FRLAP+YQHAPTVLMLLYPQYGAP++F+KI
Sbjct: 487 QAKLAIAIIVQRFSFRLAPSYQHAPTVLMLLYPQYGAPVVFRKI 529

BLAST of CSPI01G18500 vs. NCBI nr
Match: XP_011656192.2 (cytochrome P450 734A1 [Cucumis sativus] >KAE8653075.1 hypothetical protein Csa_019999 [Cucumis sativus])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MYYPFLKREKIIIMKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDR 60
           MYYPFLKREKIIIMKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDR
Sbjct: 1   MYYPFLKREKIIIMKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDR 60

Query: 61  QGIKGPPYRFFIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWF 120
           QGIKGPPYRFFIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWF
Sbjct: 61  QGIKGPPYRFFIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWF 120

Query: 121 GPIVRVAVSDPDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPT 180
           GPIVRVAVSDPDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPT
Sbjct: 121 GPIVRVAVSDPDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPT 180

Query: 181 FHVDNLKLLIPLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSS 240
           FHVDNLKLLIPLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSS
Sbjct: 181 FHVDNLKLLIPLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSS 240

Query: 241 YEDGKAIFKLQTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRK 300
           YEDGKAIFKLQTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRK
Sbjct: 241 YEDGKAIFKLQTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRK 300

Query: 301 RNLEKKNWGMNEKRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSN 360
           RNLEKKNWGMNEKRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSN
Sbjct: 301 RNLEKKNWGMNEKRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSN 360

Query: 361 LLTWTTVLLAMHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVA 420
           LLTWTTVLLAMHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVA
Sbjct: 361 LLTWTTVLLAMHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVA 420

Query: 421 TIRRAKTDVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPA 480
           TIRRAKTDVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPA
Sbjct: 421 TIRRAKTDVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPA 480

Query: 481 GFIPFGLGVRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPII 540
           GFIPFGLGVRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPII
Sbjct: 481 GFIPFGLGVRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPII 540

Query: 541 FQKIPIN 548
           FQKIPIN
Sbjct: 541 FQKIPIN 547

BLAST of CSPI01G18500 vs. NCBI nr
Match: XP_008465003.1 (PREDICTED: cytochrome P450 734A1-like [Cucumis melo])

HSP 1 Score: 1038.5 bits (2684), Expect = 2.1e-299
Identity = 513/537 (95.53%), Postives = 521/537 (97.02%), Query Frame = 0

Query: 14  MKEVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG 73
           MKEVKIESPAWSA  VT  VLLIFII+AKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG
Sbjct: 1   MKEVKIESPAWSAAAVTTAVLLIFIISAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIG 60

Query: 74  NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 133
           NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM
Sbjct: 61  NFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDM 120

Query: 134 IREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV 193
           IREIFATNS +YEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV
Sbjct: 121 IREIFATNSQYYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLV 180

Query: 194 EESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQ 253
           EESVVEMVEKWWSMAES++S EVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQ Q
Sbjct: 181 EESVVEMVEKWWSMAESVESSEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQNQ 240

Query: 254 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEK 313
           QMILASQAFQKVFIPGYRFLPTRTNINSWKLD+EIKKSLMKLIEGRK NLEKKNWGMNEK
Sbjct: 241 QMILASQAFQKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKGNLEKKNWGMNEK 300

Query: 314 RPKDLLGLMIQASLL-----NNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVL 373
           RPKDLLGLMIQASLL     NNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVL
Sbjct: 301 RPKDLLGLMIQASLLNNNNNNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVL 360

Query: 374 LAMHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTD 433
           LAMHPQWQIQARDEV NVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAK D
Sbjct: 361 LAMHPQWQIQARDEVINVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKAD 420

Query: 434 VELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLG 493
           VELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVS+A NHPAGFIPFGLG
Sbjct: 421 VELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSRAGNHPAGFIPFGLG 480

Query: 494 VRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 546
           VRTCIGQNLA+LEAKLT+AIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP
Sbjct: 481 VRTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIP 537

BLAST of CSPI01G18500 vs. NCBI nr
Match: XP_038880221.1 (cytochrome P450 734A1-like [Benincasa hispida])

HSP 1 Score: 963.8 bits (2490), Expect = 6.5e-277
Identity = 479/539 (88.87%), Postives = 504/539 (93.51%), Query Frame = 0

Query: 17  VKIES------PAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRF 76
           +KIES      P W+A V   VV+   I++AK+ LVLWWKP++IEKHFDRQGI+GPPYRF
Sbjct: 1   MKIESVLSKVPPVWAAAVTAAVVM---ILSAKMVLVLWWKPRRIEKHFDRQGIRGPPYRF 60

Query: 77  FIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 136
           FIGNFKE+VS+MLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD
Sbjct: 61  FIGNFKEVVSMMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSD 120

Query: 137 PDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLI 196
           PDMIREIF TNSHFYEKNEAHPLVKQLEGDGLLTL GQKWALHRKIISPTFH+DNLKLLI
Sbjct: 121 PDMIREIFTTNSHFYEKNEAHPLVKQLEGDGLLTLKGQKWALHRKIISPTFHMDNLKLLI 180

Query: 197 PLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKL 256
           PLVEESVVEMVEKW SMAES DS EVEIDV++ FQTLTEDVITKTAFGSSYEDGKAIFKL
Sbjct: 181 PLVEESVVEMVEKWSSMAESTDSDEVEIDVFECFQTLTEDVITKTAFGSSYEDGKAIFKL 240

Query: 257 QTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGM 316
           QTQQMILASQAF KVFIPGYRFLPTRTNINSWKLD+EIKKSLMKLIEGRK N E KNWGM
Sbjct: 241 QTQQMILASQAFHKVFIPGYRFLPTRTNINSWKLDKEIKKSLMKLIEGRKWNSE-KNWGM 300

Query: 317 NEKRPKDLLGLMIQASLL--NNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVL 376
           +EKRPKDLLGLMIQAS L  NNNNNNN+SITVHDIVEECKSFFFAGKQTTSNLLTWTTVL
Sbjct: 301 DEKRPKDLLGLMIQASSLKYNNNNNNNNSITVHDIVEECKSFFFAGKQTTSNLLTWTTVL 360

Query: 377 LAMHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTD 436
           LAMHPQWQIQARDEVFNVCG RALPSKHDVS FKILSMILNESLRLYPPTVATIRRAK D
Sbjct: 361 LAMHPQWQIQARDEVFNVCGSRALPSKHDVSKFKILSMILNESLRLYPPTVATIRRAKAD 420

Query: 437 VELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLG 496
           VEL GRMIPRGTEFLIPI+AVHHDQ+IWGNDASEFNPGRFSEGV +A NHPAGFIPFGLG
Sbjct: 421 VELGGRMIPRGTEFLIPILAVHHDQSIWGNDASEFNPGRFSEGVGRAGNHPAGFIPFGLG 480

Query: 497 VRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 548
            RTCIGQNLA+LEAKLT+AIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPI+FQKIP++
Sbjct: 481 ARTCIGQNLAILEAKLTMAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPILFQKIPLD 535

BLAST of CSPI01G18500 vs. NCBI nr
Match: XP_023516914.1 (cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 889.4 bits (2297), Expect = 1.6e-254
Identity = 439/532 (82.52%), Postives = 469/532 (88.16%), Query Frame = 0

Query: 16  EVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNF 75
           E  +E P  +A  V  V  ++ I+ AKVGLVLWWKP++IE HF RQGI GPPYRFFIGNF
Sbjct: 9   ESAVEVPFGAAAAVAAVAAVVVILGAKVGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNF 68

Query: 76  KELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR 135
           KELVS+M AASSHPMPNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIR
Sbjct: 69  KELVSMMAAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIPRVAVSDPDMIR 128

Query: 136 EIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEE 195
           EIF TNSH YEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH DNLKLLIPL+EE
Sbjct: 129 EIFITNSHLYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLIEE 188

Query: 196 SVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 255
           S+VEMV+KW SMAE   SGEVEIDV+D FQTLTEDVITKTAFG SY DGKAIFKLQTQQM
Sbjct: 189 SIVEMVQKWSSMAEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYGDGKAIFKLQTQQM 248

Query: 256 ILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRP 315
           ILAS+AFQKVFIPGYRFLPTRTN+ SWKL++EIKKSLMKLIEGRKRN E KNW +NEK P
Sbjct: 249 ILASEAFQKVFIPGYRFLPTRTNMKSWKLEKEIKKSLMKLIEGRKRNFE-KNWAINEKGP 308

Query: 316 KDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW 375
           KDLLGLMIQA           SI+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+W
Sbjct: 309 KDLLGLMIQA-----------SISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRW 368

Query: 376 QIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRM 435
           QI ARDEVF +CGPRALPS  DVS FKILSMILNESLRLYPPTVATIRRA  DV+L GRM
Sbjct: 369 QILARDEVFTICGPRALPSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVDLGGRM 428

Query: 436 IPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQ 495
           IPRGTEFLI I+AVHHDQTIWGNDASEFNP RFS+GVS+A NHP GFIPFGLGVR CIGQ
Sbjct: 429 IPRGTEFLISILAVHHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQ 488

Query: 496 NLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 548
           NLA+LEAKL + IILQR +FRLAPTYQHAPTVLMLLYPQYGAPI F+KIP+N
Sbjct: 489 NLAVLEAKLAMVIILQRLSFRLAPTYQHAPTVLMLLYPQYGAPIRFEKIPLN 528

BLAST of CSPI01G18500 vs. NCBI nr
Match: XP_022961116.1 (cytochrome P450 734A1-like [Cucurbita moschata])

HSP 1 Score: 882.1 bits (2278), Expect = 2.5e-252
Identity = 434/532 (81.58%), Postives = 469/532 (88.16%), Query Frame = 0

Query: 16  EVKIESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNF 75
           E ++ES       V    +++ I+ AK+GLVLWWKP++IE HF RQGI GPPYRFFIGNF
Sbjct: 2   EEELESAVEVPFGVAAAAVVVVILGAKMGLVLWWKPRRIENHFLRQGIGGPPYRFFIGNF 61

Query: 76  KELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIR 135
           KELVS+M AASSHP+PNFSHNILPRVLSFYHHWKKIYGSTF+IWFGPI RVAVSDPDMIR
Sbjct: 62  KELVSMMAAASSHPIPNFSHNILPRVLSFYHHWKKIYGSTFVIWFGPIARVAVSDPDMIR 121

Query: 136 EIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEE 195
           EIF TNSHFYEKNEAHPLVKQLEGDGLLTL G KWALHRKIISPTFH DNLKLLIPLVEE
Sbjct: 122 EIFITNSHFYEKNEAHPLVKQLEGDGLLTLNGPKWALHRKIISPTFHTDNLKLLIPLVEE 181

Query: 196 SVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQM 255
           SV++MV+KW SM E   SGEVEIDV+D FQTLTEDVITKTAFG SYEDGKAIFK+QTQQM
Sbjct: 182 SVMDMVQKWSSMTEETTSGEVEIDVFDSFQTLTEDVITKTAFGCSYEDGKAIFKIQTQQM 241

Query: 256 ILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRP 315
           ILAS+AFQKVFIPGYRFLPTRTN+ SWKL++EIKKSL+KLIEGRKRN E KNW +NEK P
Sbjct: 242 ILASEAFQKVFIPGYRFLPTRTNMKSWKLEKEIKKSLLKLIEGRKRNFE-KNWAINEKGP 301

Query: 316 KDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQW 375
           KDLLGLMIQA           SI+VHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHP+W
Sbjct: 302 KDLLGLMIQA-----------SISVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPRW 361

Query: 376 QIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRM 435
           QI AR EVF +CGPRALPS  DVS FKILSMILNESLRLYPPTVATIRRA  DVEL GRM
Sbjct: 362 QILARHEVFTICGPRALPSSDDVSKFKILSMILNESLRLYPPTVATIRRANADVELGGRM 421

Query: 436 IPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQ 495
           IPRGTEFLIPI+AVHHDQTIWGNDASEFNP RFS+GVS+A NHP GFIPFGLGVR CIGQ
Sbjct: 422 IPRGTEFLIPILAVHHDQTIWGNDASEFNPERFSDGVSRAGNHPVGFIPFGLGVRNCIGQ 481

Query: 496 NLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKIPIN 548
           NLA+LEAKL + IILQR +FRLAPTYQHAPTVLMLLYPQYGAPI+F+KI +N
Sbjct: 482 NLAVLEAKLAMVIILQRLSFRLAPTYQHAPTVLMLLYPQYGAPILFEKIALN 521

BLAST of CSPI01G18500 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 750.7 bits (1937), Expect = 8.0e-217
Identity = 361/526 (68.63%), Postives = 437/526 (83.08%), Query Frame = 0

Query: 20  ESPAWSATVVTIVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELV 79
           ES +W    V ++ +++ ++  K   +LWW+P+KIE+HF +QGI+GPPY FFIGN KELV
Sbjct: 4   ESSSWFIPKVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELV 63

Query: 80  SLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFA 139
            +ML ASSHPMP FSHNILPRVLSFYHHW+KIYG+TFL+WFGP  R+ V+DPD+IREIF 
Sbjct: 64  GMMLKASSHPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF- 123

Query: 140 TNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVE 199
           + S FYEKNEAHPLVKQLEGDGLL+L G+KWA HRKIISPTFH++NLKLL+P+V +SV +
Sbjct: 124 SKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTD 183

Query: 200 MVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILAS 259
           MV+KW    +  ++GEVE+DVY+WFQ LTEDVI++TAFGSSYEDG+A+F+LQ QQM+L +
Sbjct: 184 MVDKW--SDKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCA 243

Query: 260 QAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRN-LEKKNWGMNEKRPKDL 319
           +AFQKVFIPGYRF PTR N+ SWKLD+EI+KSL+KLIE R++N ++ +     E   KDL
Sbjct: 244 EAFQKVFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDL 303

Query: 320 LGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQ 379
           LGLMIQA           ++TV DIVEECKSFFFAGKQTTSNLLTWTT+LL+MHP+WQ +
Sbjct: 304 LGLMIQA----------KNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAK 363

Query: 380 ARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRMIPR 439
           ARDEV  VCG R +P+K  V   K LSMILNESLRLYPP VATIRRAK+DV+L G  IP 
Sbjct: 364 ARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPC 423

Query: 440 GTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQNLA 499
           GTE LIPIIAVHHDQ IWGND +EFNP RF++GV +AA HP GFIPFGLGVRTCIGQNLA
Sbjct: 424 GTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLA 483

Query: 500 LLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 545
           +L+AKLTLA+++QRFTF LAPTYQHAPTVLMLLYPQ+GAPI F+++
Sbjct: 484 ILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

BLAST of CSPI01G18500 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-115
Identity = 215/533 (40.34%), Postives = 328/533 (61.54%), Query Frame = 0

Query: 14  MKEVKIESPAWSATVVTIVVLLIFIITAKVGL-VLWWKPKKIEKHFDRQGIKGPPYRFFI 73
           M + KI + A +A VV +  + ++I     GL V W +PKK E +  RQG+ G P+ F +
Sbjct: 1   MSDTKISAVAVAAAVVVVTTVTVWIWK---GLNVAWLRPKKNEAYLKRQGLSGTPFTFLV 60

Query: 74  GNFKELVSLMLAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPD 133
           G+ K   S++    S P+ N + +   RV+       K +G T  +W GPI  V V+ P+
Sbjct: 61  GDIKREASMVEQEKSRPI-NLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPE 120

Query: 134 MIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPL 193
            I+++     + + K   HP+V +L   G+    G+KW+ HRKII+P+FH++ LK++IP 
Sbjct: 121 HIKDVL-NRVYDFPKPPVHPIV-ELFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPA 180

Query: 194 VEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQT 253
             ES  EM+ KW  +     S   EIDV+ +   LT DVI++TAFGSSYE+GK IF+LQ 
Sbjct: 181 FYESCSEMISKWEKLVTEQGSSN-EIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQE 240

Query: 254 QQMILASQAFQKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNE 313
           +Q     +A +  FIPG RFLPT+ N+   ++++E+K  L ++I  R+R ++       E
Sbjct: 241 EQGRRVLKALELAFIPGMRFLPTKNNLRMRQINKEVKSRLREIIMKRQRGMD-----TGE 300

Query: 314 KRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMH 373
               DLLG++++      +N+ +  +++ D+VEEC+ F FAG++TT+ LL WT ++L+ H
Sbjct: 301 APKNDLLGILLE------SNSGDHGMSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHH 360

Query: 374 PQWQIQARDEVFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELA 433
            +WQ QAR+E+  V G    P+   +S  K +SMILNE LRLYPP +   R  + + +L 
Sbjct: 361 QKWQDQAREEILKVIGKNNKPNFDALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLG 420

Query: 434 GRM-IPRGTEFLIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRT 493
             M +P G + +IP++ VH D  +WG D  EFNP RF++G+SKA  +   F+PFG G R 
Sbjct: 421 EDMTLPGGAQVVIPVLMVHRDPELWGEDVHEFNPERFADGISKATKNQVSFLPFGWGPRF 480

Query: 494 CIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 545
           C GQN AL+EAK+ L +ILQRF+F L+P+Y HAP  ++ L+PQ+GAP+IF  +
Sbjct: 481 CPGQNFALMEAKMALVLILQRFSFELSPSYTHAPHTVLTLHPQFGAPLIFHML 515

BLAST of CSPI01G18500 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.8e-112
Identity = 197/522 (37.74%), Postives = 316/522 (60.54%), Query Frame = 0

Query: 26  ATVVTIVVLLIFIITAKV---GLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELVSLM 85
           +T+  + + L+ ++  K+     +L W+P  + + F +QGI GP YR   GN +E+  + 
Sbjct: 60  STINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMK 119

Query: 86  LAASSHPMPNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFATNS 145
             A    +   S++I+PRVL     WK  YG TFL W G   R+ +SD ++ ++I +   
Sbjct: 120 NEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKF 179

Query: 146 HFYEKNEAHPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVEMVE 205
            F+ K++  P + +L G+GL+ + G  W  HR+I++P F +D LKL+  L+ +    M  
Sbjct: 180 VFFSKSKTKPEILKLSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFL 239

Query: 206 KWWSMAESMDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAF 265
           +W      +++ E  + +   F+ LT D+I   AFGSSY +G  +FK Q +     + A 
Sbjct: 240 EWKKQRNGVET-EQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAAL 299

Query: 266 QKVFIPGYRFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRPKDLLGLM 325
             ++ PG ++LPT +N+  WKLD ++  S+ ++I+ R  + E K++G       DLLG+M
Sbjct: 300 TDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARLTS-ESKDYG------NDLLGIM 359

Query: 326 IQASLLNNNNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDE 385
           + A+   ++N +   +++ +I+EECK+FFFAG +TT+NLLTW+T+LL++H  WQ + R+E
Sbjct: 360 LTAA---SSNESEKKMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREE 419

Query: 386 VFNVCGPRALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRMIPRGTEF 445
           VFN CG   +P     S  K+++ +  ESLRLY P +  +R A  D++L    IP+GT  
Sbjct: 420 VFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTI 479

Query: 446 LIPIIAVHHDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQNLALLEA 505
           ++PI  +H D+ +WG+DA +FNP RF+ G+S+AANHP   + F +G R CIGQN A++EA
Sbjct: 480 ILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEA 539

Query: 506 KLTLAIILQRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 545
           K  LA+ILQRF   L+  Y+HAP   + L PQY  P+I + I
Sbjct: 540 KTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPI 570

BLAST of CSPI01G18500 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 402.1 bits (1032), Expect = 7.0e-112
Identity = 204/514 (39.69%), Postives = 315/514 (61.28%), Query Frame = 0

Query: 31  IVVLLIFIITAKVGLVLWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELVSLMLAASSHPM 90
           ++V ++ + T ++   +W KPK +E    RQG+ G PY   +G+ K  V +M+ A S P+
Sbjct: 11  VLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEARSKPI 70

Query: 91  PNFSHNILPRVLSFYHHWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFATNSHFYEKNEA 150
            N + +I PR+L         +G TF IW GP+  + +++P+ I+E+F   + F EK   
Sbjct: 71  -NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDF-EKAST 130

Query: 151 HPLVKQLEGDGLLTLTGQKWALHRKIISPTFHVDNLKLLIPLVEESVVEMVEKWWSMAES 210
            PL++ L G GL +  G KWA HR+II+P FH++ +K +IP       E+V +W  +   
Sbjct: 131 FPLIRLLAG-GLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLFTD 190

Query: 211 MDSGEVEIDVYDWFQTLTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFIPGY 270
            +S  +E+DV+ W   +T DVI+ TAFGSSY++G+ IF+LQ +   L +QAF+K +IPG 
Sbjct: 191 KES-PLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKKSYIPGS 250

Query: 271 RFLPTRTNINSWKLDREIKKSLMKLIEGRKRNLEKKNWGMNEKRPKDLLGLMIQASLLNN 330
           RF PT++N     +DRE+   L  ++  R++  E       E    DLLG+++++   N+
Sbjct: 251 RFYPTKSNRRMKAIDREVDVILRGIVSKREKARE-----AGEPANDDLLGILLES---NS 310

Query: 331 NNNNNSSITVHDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGPR 390
             +  + ++V D+++ECK F+FAG++TTS LL WT VLL+ H  WQ +AR+EV  V G  
Sbjct: 311 EESQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGEN 370

Query: 391 ALPSKHDVSNFKILSMILNESLRLYPPTVATIRRAKTDVELAGRMIPRGTEFLIPIIAVH 450
             P    ++N K+++MI NE LRLYPP     R    +++L    +P G +  +P I V 
Sbjct: 371 NKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILVQ 430

Query: 451 HDQTIWGNDASEFNPGRFSEGVSKAANHPAGFIPFGLGVRTCIGQNLALLEAKLTLAIIL 510
            D  +WG+DA++F P RF +G+SKA  +   F PFG G R CIGQN A+LEAK+ +A+IL
Sbjct: 431 RDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMALIL 490

Query: 511 QRFTFRLAPTYQHAPTVLMLLYPQYGAPIIFQKI 545
           Q+F+F L+P+Y HAP  +M   PQ+GA +I  K+
Sbjct: 491 QKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of CSPI01G18500 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 400.6 bits (1028), Expect = 2.0e-111
Identity = 207/498 (41.57%), Postives = 306/498 (61.45%), Query Frame = 0

Query: 47  LWWKPKKIEKHFDRQGIKGPPYRFFIGNFKELVSLMLAASSHPMPNFSHNILPRVLSFYH 106
           +W+ PK +E+   RQG+ G  Y   IG+FK+++S+ + A+S P+   + +I PRV+    
Sbjct: 28  VWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKPIKP-TDDITPRVMPHPL 87

Query: 107 HWKKIYGSTFLIWFGPIVRVAVSDPDMIREIFATNSHFYEKNEAHPLVKQLEGDGLLTLT 166
              K +G T L WFGPI  + + DP+ I+E+F    + ++K    PL K L G GL++  
Sbjct: 88  QMLKTHGRTNLTWFGPIPTITIMDPEQIKEVF-NKVYDFQKAHTFPLSKIL-GTGLVSYD 147

Query: 167 GQKWALHRKIISPTFHVDNLKLLIPLVEESVVEMVEKWWSMAESMDSGEVEIDVYDWFQT 226
           G KWA HR+II+P FH++ +K ++ +  ES  E+V +W  +     S   E+DV+    +
Sbjct: 148 GDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKGS-SCEVDVWPGLTS 207

Query: 227 LTEDVITKTAFGSSYEDGKAIFKLQTQQMILASQAFQKVFIPGYRFLPTRTNINSWKLDR 286
           +T DVI++TAFGSSY +G  IF+LQ +   L  QAFQK FIPGY +LPT+ N       R
Sbjct: 208 MTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYLPTKGNRRMKTAAR 267

Query: 287 EIKKSLMKLIEGRKRNLEKKNWGMNEKRPKDLLGLMIQASLLNNNNNNNSSITVHDIVEE 346
           EI+  L  +I  R+R  E       E   +DLLG++++++L     N  S+    D++EE
Sbjct: 268 EIQDILRGIINKRERARES-----GEAPSEDLLGILLESNLGQTEGNGMST---EDMMEE 327

Query: 347 CKSFFFAGKQTTSNLLTWTTVLLAMHPQWQIQARDEVFNVCGPRALPSKHDVSNFKILSM 406
           CK F+ AG++TTS LL WT VLL+ H  WQ +AR+EV  V G +  P    ++  K+++M
Sbjct: 328 CKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQ-PDTEGLNQLKVMTM 387

Query: 407 ILNESLRLYPPTVATIRRAKTDVELAGRMIPRGTEFLIPIIAVHHDQTIWGNDASEFNPG 466
           IL E LRLYPP V   R    +++L    +P G +  +P++ VH D  +WGNDA EF P 
Sbjct: 388 ILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELWGNDAGEFKPE 447

Query: 467 RFSEGVSKAANHPAGFIPFGLGVRTCIGQNLALLEAKLTLAIILQRFTFRLAPTYQHAPT 526
           RF +G+SKA  +   F PF  G R CIGQN  LLEAK+ +++ILQRF+F L+P+Y HAP 
Sbjct: 448 RFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFELSPSYVHAPY 507

Query: 527 VLMLLYPQYGAPIIFQKI 545
            ++ LYPQ+GA ++  K+
Sbjct: 508 TIITLYPQFGAHLMLHKL 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O487861.1e-21568.63Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
B9X2872.6e-18862.06Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ... [more]
Q6Z6D67.9e-17756.79Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... [more]
Q69XM61.5e-16754.07Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 ... [more]
Q8LIF21.1e-14647.85Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LU200.0e+00100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G305710 PE=3 SV=1[more]
A0A1S3CMW01.0e-29995.53cytochrome P450 734A1-like OS=Cucumis melo OX=3656 GN=LOC103502730 PE=3 SV=1[more]
A0A6J1H9G11.2e-25281.58cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111461725 PE=3 SV... [more]
A0A6J1JHK63.3e-25081.02cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111485166 PE=3 SV=1[more]
A0A6J1EZI11.7e-24680.15cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440975 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_011656192.20.0e+00100.00cytochrome P450 734A1 [Cucumis sativus] >KAE8653075.1 hypothetical protein Csa_0... [more]
XP_008465003.12.1e-29995.53PREDICTED: cytochrome P450 734A1-like [Cucumis melo][more]
XP_038880221.16.5e-27788.87cytochrome P450 734A1-like [Benincasa hispida][more]
XP_023516914.11.6e-25482.52cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo][more]
XP_022961116.12.5e-25281.58cytochrome P450 734A1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G26710.18.0e-21768.63Cytochrome P450 superfamily protein [more]
AT3G14620.11.8e-11540.34cytochrome P450, family 72, subfamily A, polypeptide 8 [more]
AT2G46950.11.8e-11237.74cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT3G14610.17.0e-11239.69cytochrome P450, family 72, subfamily A, polypeptide 7 [more]
AT3G14680.12.0e-11141.57cytochrome P450, family 72, subfamily A, polypeptide 14 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 342..359
score: 30.53
coord: 109..128
score: 29.15
coord: 227..245
score: 21.49
coord: 492..515
score: 41.18
coord: 362..388
score: 28.07
coord: 482..492
score: 54.13
IPR001128Cytochrome P450PRINTSPR00385P450coord: 492..503
score: 44.98
coord: 483..492
score: 56.5
coord: 353..370
score: 38.91
coord: 406..417
score: 39.28
IPR001128Cytochrome P450PFAMPF00067p450coord: 102..525
e-value: 1.9E-87
score: 294.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 47..543
e-value: 3.3E-117
score: 393.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 65..542
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 29..539
NoneNo IPR availablePANTHERPTHR24282:SF63CYTOCHROMEA2coord: 29..539
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 485..494

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G18500.1CSPI01G18500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016131 brassinosteroid metabolic process
biological_process GO:0009741 response to brassinosteroid
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0008395 steroid hydroxylase activity
molecular_function GO:0004497 monooxygenase activity