CSPI01G17990 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G17990
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	DNA-directed RNA polymerase subunit beta
Location	Chr1: 13464774 .. 13465835 (+)
RNA-Seq Expression	CSPI01G17990
Synteny	CSPI01G17990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGTTAGCAAGAGAGTTTGTTAAGAGTGAAAAATCTAAAATGATAAAACTGCTTAAGAGAAGAGAAGTTAGAGAAAGAAAGCTTTTCGTGCTGTTTAAATTTTAGTGCATATTTTGTAAATATTTTCTTCTTTCTGTTTACCATAATTCCTCAATCTGTAATTCCCTTAAAAATTTAATGGTAGAATTCGTACCCCCCCCACTTGTTCTCTGTTTCTAATGGAAAAAGGATGCGGCCGTAATTCATTTCCACAGGCATTCATTTCAGATTTTCCATAACTGTCTTAACTTCAATTGGCAATGGCGATGGTAGATTCAAGTTTTATCTTCATCGGTTTCTCCCCCGTACCTCATCCCCTTTTGAACAACAATAATGGCAGAAAATTCGGAATCACGTCGGCAAAACGTACACAAGACTCCGATTCCGATTCCGCTTCTTCAACCCAACCAAATCAATTCAAATTTCCATCTCTGAGAGTATCAAATCCACTTCTAGCCCGATCTGTCGTTTCGGTCCTCGGATTAGGGTTCGTCGATGCAGGGTACGAAAATTTCACCTCTAATTAATTTCGATTTTGAGAATTGCTCTGTTTTATTTTTGTTTTCACTGAAACTGAAGTCGAATTAGGGTTTGGAATGATTAGGTATAGCGGAGATTGGTCTAGAATTGGAGCGATTACGAAGGAAACTGAAGATTTGCTTAAGATTGGAGCTTTGTTGGTTGTTCCGTTTTGTGTATTTCTCGTATTTTCTTTCTCAAAATATGATTCGGATTCTTCGTAAGTGTATGGATGATGGATTCCCCAAAATGGTTAGAAATAATTTCAATTTGAAAATGGTAGCTAAGCCAAAGTTGCTTTTTTTGGTTAGAAGGGAGATTTTGAAGAACATGTACAAAATTCATTTACTGATATGCACATGGTTCTTGTTGGTTTCCACTCTCATGTTTTAGAATTTAGTAAGCGTCACACTGTTTAGAAATAGTCATATTCGAACAAGAAATGATATCTAGGTGCATACATTCACTTTAGTAGCTTATTGGCTAAAGTCACTATGCGAATA

mRNA sequence

GAAGTTAGCAAGAGAGTTTGTTAAGAGTGAAAAATCTAAAATGATAAAACTGCTTAAGAGAAGAGAAGTTAGAGAAAGAAAGCTTTTCGTGCTGTTTAAATTTTAGTGCATATTTTGTAAATATTTTCTTCTTTCTGTTTACCATAATTCCTCAATCTGTAATTCCCTTAAAAATTTAATGGTAGAATTCGTACCCCCCCCACTTGTTCTCTGTTTCTAATGGAAAAAGGATGCGGCCGTAATTCATTTCCACAGGCATTCATTTCAGATTTTCCATAACTGTCTTAACTTCAATTGGCAATGGCGATGGTAGATTCAAGTTTTATCTTCATCGGTTTCTCCCCCGTACCTCATCCCCTTTTGAACAACAATAATGGCAGAAAATTCGGAATCACGTCGGCAAAACGTACACAAGACTCCGATTCCGATTCCGCTTCTTCAACCCAACCAAATCAATTCAAATTTCCATCTCTGAGAGTATCAAATCCACTTCTAGCCCGATCTGTCGTTTCGGTCCTCGGATTAGGGTTCGTCGATGCAGGGTATAGCGGAGATTGGTCTAGAATTGGAGCGATTACGAAGGAAACTGAAGATTTGCTTAAGATTGGAGCTTTGTTGGTTGTTCCGTTTTGTGTATTTCTCGTATTTTCTTTCTCAAAATATGATTCGGATTCTTCGTAAGTGTATGGATGATGGATTCCCCAAAATGGTTAGAAATAATTTCAATTTGAAAATGGTAGCTAAGCCAAAGTTGCTTTTTTTGGTTAGAAGGGAGATTTTGAAGAACATGTACAAAATTCATTTACTGATATGCACATGGTTCTTGTTGGTTTCCACTCTCATGTTTTAGAATTTAGTAAGCGTCACACTGTTTAGAAATAGTCATATTCGAACAAGAAATGATATCTAGGTGCATACATTCACTTTAGTAGCTTATTGGCTAAAGTCACTATGCGAATA

Coding sequence (CDS)

ATGGCGATGGTAGATTCAAGTTTTATCTTCATCGGTTTCTCCCCCGTACCTCATCCCCTTTTGAACAACAATAATGGCAGAAAATTCGGAATCACGTCGGCAAAACGTACACAAGACTCCGATTCCGATTCCGCTTCTTCAACCCAACCAAATCAATTCAAATTTCCATCTCTGAGAGTATCAAATCCACTTCTAGCCCGATCTGTCGTTTCGGTCCTCGGATTAGGGTTCGTCGATGCAGGGTATAGCGGAGATTGGTCTAGAATTGGAGCGATTACGAAGGAAACTGAAGATTTGCTTAAGATTGGAGCTTTGTTGGTTGTTCCGTTTTGTGTATTTCTCGTATTTTCTTTCTCAAAATATGATTCGGATTCTTCGTAA

Protein sequence

MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSKYDSDSS*

Homology

[Close]

BLAST of CSPI01G17990 vs. ExPASy TrEMBL
Match: A0A0A0LWH4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G257930 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.1e-63
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60
           MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV
Sbjct: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60

Query: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120
           SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK
Sbjct: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120

Query: 121 YDSDSS 127
           YDSDSS
Sbjct: 121 YDSDSS 126

[Close]

BLAST of CSPI01G17990 vs. ExPASy TrEMBL
Match: A0A1S3B903 (uncharacterized protein LOC103487329 OS=Cucumis melo OX=3656 GN=LOC103487329 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.4e-55
Identity = 118/129 (91.47%), Postives = 120/129 (93.02%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHPLLN---NNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPS 60
           MAMVDSSFIFIG S VPHPLLN   NNNGRKFGITSAKRTQ  DSDS SSTQPNQFKFPS
Sbjct: 1   MAMVDSSFIFIGISAVPHPLLNNNKNNNGRKFGITSAKRTQ--DSDSTSSTQPNQFKFPS 60

Query: 61  LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFS 120
           LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETE+LLKIGA LVVPFCVFLVFS
Sbjct: 61  LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEELLKIGAFLVVPFCVFLVFS 120

Query: 121 FSKYDSDSS 127
           FSK+DSDSS
Sbjct: 121 FSKFDSDSS 127

[Close]

BLAST of CSPI01G17990 vs. ExPASy TrEMBL
Match: A0A6J1EZT4 (uncharacterized protein LOC111440861 OS=Cucurbita moschata OX=3662 GN=LOC111440861 PE=4 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 8.0e-43
Identity = 100/127 (78.74%), Postives = 108/127 (85.04%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHP-LLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLR 60
           M + DSSFIF G S +PHP LL+NNN RKFG TSAKRT   DSDS SST   Q KFPSLR
Sbjct: 1   MPIADSSFIFTGLSALPHPLLLSNNNARKFGTTSAKRT--GDSDSISST---QLKFPSLR 60

Query: 61  VSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFS 120
           V+NPLLARSVV+VLGLGFVDAGYSGDWSRIGAITKETEDLLKIGA LVVPFCVFL+FSFS
Sbjct: 61  VANPLLARSVVAVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGAFLVVPFCVFLIFSFS 120

Query: 121 KYDSDSS 127
           +  +DSS
Sbjct: 121 E-QTDSS 121

[Close]

BLAST of CSPI01G17990 vs. ExPASy TrEMBL
Match: A0A6J1KT82 (uncharacterized protein LOC111498016 OS=Cucurbita maxima OX=3661 GN=LOC111498016 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.1e-42
Identity = 100/127 (78.74%), Postives = 107/127 (84.25%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHPL-LNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLR 60
           M + DSSFIF G S +PHPL L+NNN RKFG TSAKRT   DSDS SST   Q KFPSLR
Sbjct: 1   MPIADSSFIFTGLSALPHPLILSNNNARKFGTTSAKRT--GDSDSISST---QLKFPSLR 60

Query: 61  VSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFS 120
           VSNPLLARSVV+VLGLGFVDAGYSGDWSRIGAITKETED LKIGA LVVPFCVFL+FSFS
Sbjct: 61  VSNPLLARSVVAVLGLGFVDAGYSGDWSRIGAITKETEDFLKIGAFLVVPFCVFLIFSFS 120

Query: 121 KYDSDSS 127
           +  +DSS
Sbjct: 121 E-QTDSS 121

[Close]

BLAST of CSPI01G17990 vs. ExPASy TrEMBL
Match: A0A6J1C0R6 (uncharacterized protein LOC111007301 OS=Momordica charantia OX=3673 GN=LOC111007301 PE=4 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.6e-35
Identity = 87/124 (70.16%), Postives = 95/124 (76.61%), Query Frame = 0


Query: 3   MVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRVSN 62
           M DS+FIF G S V   L N NN R F    AKRT   DS +  S Q NQF+FPSLRV N
Sbjct: 1   MADSTFIFSGPSAVSRSLFNVNNRRNFRTALAKRT--DDSTATKSNQENQFEFPSLRVPN 60

Query: 63  PLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSKYD 122
           PL+AR VVSVLGLGFVDAGYSGDWSRIGAITKE+EDLLKIGA LVVPFCVFL+ SF+K  
Sbjct: 61  PLVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAK-Q 120

Query: 123 SDSS 127
           +DSS
Sbjct: 121 TDSS 121

[Close]

BLAST of CSPI01G17990 vs. NCBI nr
Match: XP_004146667.1 (uncharacterized protein LOC101206545 [Cucumis sativus] >KGN65187.1 hypothetical protein Csa_020133 [Cucumis sativus])

HSP 1 Score: 250.8 bits (639), Expect = 6.5e-63
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60
           MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV
Sbjct: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60

Query: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120
           SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK
Sbjct: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120

Query: 121 YDSDSS 127
           YDSDSS
Sbjct: 121 YDSDSS 126

[Close]

BLAST of CSPI01G17990 vs. NCBI nr
Match: XP_008443827.1 (PREDICTED: uncharacterized protein LOC103487329 [Cucumis melo])

HSP 1 Score: 225.3 bits (573), Expect = 2.9e-55
Identity = 118/129 (91.47%), Postives = 120/129 (93.02%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHPLLN---NNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPS 60
           MAMVDSSFIFIG S VPHPLLN   NNNGRKFGITSAKRTQ  DSDS SSTQPNQFKFPS
Sbjct: 1   MAMVDSSFIFIGISAVPHPLLNNNKNNNGRKFGITSAKRTQ--DSDSTSSTQPNQFKFPS 60

Query: 61  LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFS 120
           LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETE+LLKIGA LVVPFCVFLVFS
Sbjct: 61  LRVSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEELLKIGAFLVVPFCVFLVFS 120

Query: 121 FSKYDSDSS 127
           FSK+DSDSS
Sbjct: 121 FSKFDSDSS 127

[Close]

BLAST of CSPI01G17990 vs. NCBI nr
Match: XP_038879768.1 (uncharacterized protein LOC120071517 [Benincasa hispida])

HSP 1 Score: 198.4 bits (503), Expect = 3.8e-47
Identity = 104/126 (82.54%), Postives = 111/126 (88.10%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60
           MAMV+SSFIFIG S VP+PL NNN+ RKF ITSAK T+DSDSDS SS    Q KFPSLRV
Sbjct: 27  MAMVNSSFIFIGVSTVPYPLSNNNHSRKFRITSAKLTEDSDSDSKSS----QLKFPSLRV 86

Query: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120
           SNPLLARSVVSVLGLGFVDAGYSGDWSRIG ITKE+E+LLKIGA LVVPFCVFLVFSFS+
Sbjct: 87  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGVITKESEELLKIGAFLVVPFCVFLVFSFSR 146

Query: 121 YDSDSS 127
            DSDSS
Sbjct: 147 LDSDSS 148

[Close]

BLAST of CSPI01G17990 vs. NCBI nr
Match: XP_023531156.1 (uncharacterized protein LOC111793484 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 189.5 bits (480), Expect = 1.8e-44
Identity = 101/127 (79.53%), Postives = 110/127 (86.61%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHP-LLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLR 60
           M + DS+FIF G S +PHP LL+NNN RKFG TSAKRT DSDSDS SST   Q KFPSLR
Sbjct: 1   MPIADSTFIFTGLSALPHPLLLSNNNARKFGTTSAKRTGDSDSDSISST---QLKFPSLR 60

Query: 61  VSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFS 120
           V+NPLLARSVV+VLGLGFVDAGYSGDWSRIGAITKETEDLLKIGA LVVPFCVFL+FSFS
Sbjct: 61  VANPLLARSVVAVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGAFLVVPFCVFLIFSFS 120

Query: 121 KYDSDSS 127
           +  +DSS
Sbjct: 121 E-QTDSS 123

[Close]

BLAST of CSPI01G17990 vs. NCBI nr
Match: XP_022933449.1 (uncharacterized protein LOC111440861 [Cucurbita moschata] >KAG6587801.1 hypothetical protein SDJN03_16366, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035696.1 hypothetical protein SDJN02_02494, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 183.0 bits (463), Expect = 1.7e-42
Identity = 100/127 (78.74%), Postives = 108/127 (85.04%), Query Frame = 0


Query: 1   MAMVDSSFIFIGFSPVPHP-LLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLR 60
           M + DSSFIF G S +PHP LL+NNN RKFG TSAKRT   DSDS SST   Q KFPSLR
Sbjct: 1   MPIADSSFIFTGLSALPHPLLLSNNNARKFGTTSAKRT--GDSDSISST---QLKFPSLR 60

Query: 61  VSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFS 120
           V+NPLLARSVV+VLGLGFVDAGYSGDWSRIGAITKETEDLLKIGA LVVPFCVFL+FSFS
Sbjct: 61  VANPLLARSVVAVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGAFLVVPFCVFLIFSFS 120

Query: 121 KYDSDSS 127
           +  +DSS
Sbjct: 121 E-QTDSS 121

[Close]

BLAST of CSPI01G17990 vs. TAIR 10
Match: AT4G30845.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; Has 16 Blast hits to 16 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 16; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 98.6 bits (244), Expect = 3.8e-21
Identity = 55/117 (47.01%), Postives = 75/117 (64.10%), Query Frame = 0


Query: 10  FIGFSPVPHPLLNNNNGRKF--GITSAKRTQDSDSDSASSTQPNQFKFPSLRVSNPLLAR 69
           F+ F P      N+N  R F  G+T   + +D  S+ ++  +P       ++V N ++AR
Sbjct: 9   FVSFCP------NDNKLRSFANGVTIMSKRRDF-SEKSNEERP----ILRIKVPNTIVAR 68

Query: 70  SVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSKYDSD 125
           S ++VL LGF+DAGYSGDWSRIG I+KETE+LLKI A LVVP C+FL  SFS   +D
Sbjct: 69  SAIAVLSLGFIDAGYSGDWSRIGVISKETEELLKIAAFLVVPLCIFLALSFSNDSTD 114

The following BLAST results are available for this feature:

BLAST of CSPI01G17990 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI01G17990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWH4	3.1e-63	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G257930 PE=4 SV=1	[more]
A0A1S3B903	1.4e-55	91.47	uncharacterized protein LOC103487329 OS=Cucumis melo OX=3656 GN=LOC103487329 PE=...	[more]
A0A6J1EZT4	8.0e-43	78.74	uncharacterized protein LOC111440861 OS=Cucurbita moschata OX=3662 GN=LOC1114408...	[more]
A0A6J1KT82	1.1e-42	78.74	uncharacterized protein LOC111498016 OS=Cucurbita maxima OX=3661 GN=LOC111498016...	[more]
A0A6J1C0R6	1.6e-35	70.16	uncharacterized protein LOC111007301 OS=Momordica charantia OX=3673 GN=LOC111007...	[more]

BLAST of CSPI01G17990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146667.1	6.5e-63	100.00	uncharacterized protein LOC101206545 [Cucumis sativus] >KGN65187.1 hypothetical ...	[more]
XP_008443827.1	2.9e-55	91.47	PREDICTED: uncharacterized protein LOC103487329 [Cucumis melo]	[more]
XP_038879768.1	3.8e-47	82.54	uncharacterized protein LOC120071517 [Benincasa hispida]	[more]
XP_023531156.1	1.8e-44	79.53	uncharacterized protein LOC111793484 [Cucurbita pepo subsp. pepo]	[more]
XP_022933449.1	1.7e-42	78.74	uncharacterized protein LOC111440861 [Cucurbita moschata] >KAG6587801.1 hypothet...	[more]

BLAST of CSPI01G17990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30845.1	3.8e-21	47.01	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..54
None	No IPR available	PANTHER	PTHR38389	DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA	coord: 1..124

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G17990.1	CSPI01G17990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane