CSPI01G15040 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G15040
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	Chr1: 10662730 .. 10664764 (+)
RNA-Seq Expression	CSPI01G15040
Synteny	CSPI01G15040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGACATCACTATTCCAGATATATATTTAAAGAGTTTATATAGTAATTGTATTTGAGAACAAGTAATATTACTTTTGGACAAAGAAGCAGCAAGCCATAGCCATGGGCGTCCCCTTGCTTCCAGCTGCTGCAAGAAGGTATGTCTACTCTCTTCCAAAAACTTTGCAATCCTTTTATTATCAAAAAGAAAAAAGGTTTTGATTTTTGGTTTTATGATTATCTAATTAGCTTCCTTTGAAATATTCTTTCTAGACAACAGGTAAAGTTGAGTTGAACTTTTAATCTTTGACGTTTAAGTTGATGATACACGTTAAACCCTTCCTTTGAATTTGTGGTTTACGGTTTCTAAATTAAAGTCTATGAACTATTGGAATTCTCAACAAGGAAGGAGCAATGGTAGCCTTAACCAAGTTTACCATAGGAAGGAAAAAATAGTTTTACACCGATTTATTTTAATTTTAGTTTTTTTCTTTAAGAAAAAAAAGACAAGGCAAACAAATTGTATGCTTTGCAATTTGCACCATTTTTATCTTTTCTCAAAGCACATTTGATTTTTTAAAATTAATTTTCCAAAGTTTATGAACAATTTCAAAAACGAACCCTACAAATCTTAACAACTTTTTAATTAATAATTATGGGTTTTGTTGTAGAAGTGTCATTCGTCCCTCCATTTTTTTTAGTCCAAATATAATAAAAGCCCTAACAACTTCCATAGAAGAATACACAAAATCCAATTACATCTAACATTTACTTTGTCATCAATCTCCTCCAAGAATCCTACCTTTTCAATTTTCAGATTTATGGGGTCTTGGGATTAAGAGCTAAAAGAAGACAAAAAATAAACGCAAGCAGTACATATAGTAACCTAAACTGAATTTTTTTTTTTGCATATTTCATGGTGGGAGCGATGAGGGTGTAGCTTCAAAGTGGGAGAAAAAAAATAAAACATTAGATAAAATGAAATGAAAAATGAAAGGTTGATATTTGCAAAAGAAGAGAAGTTGCAAAGAAAGAAATAAAAGAAAGATGAAACAATTTTTCTATAAAAAGCAATTAAAATTAAAACACATACGTGACATCATAAGAATCTCCCACGAGGCAGAGATGTTAAGGCTGGAGCACTGTAAGAGCAAGATTGTTCAAAACATGTCACAATTCGTATTGCAGAATGCTTTAAAATTCATTTTTCACATTCATATAATTAATAATATCTAACAATTCAAACATTTGGTGCACTGTATGTTAGACTAGAAGGCAAAGTGGCAATAATCACAGGTGGAGCAAGAGGAATAGGAGAGTCCATTGCAAAACACTTCTTTAAGCATGGAGCAAAAGTTGTAATTGCAGACATCCTAGACGATTTAGGTAACTCTCTTTCCAACCATCTCTCCTCTTCATCTACTTCCTTCGTCCACTGCAACGTAACGAAAGAAACCGACGTCGAAAATGTCGTTAACACTGCCGTTTCCAAATACGGAAAGCTAGACATCATGTTCAACAACGCAGGCATTCCAGGAGCTCTCAAGTTCAACATACTCGAAAACGAATATTCAGACTTCCAAAACGTACTCAATGTCAACCTAGTTGGAGCCTTTTTAGGCACAAAACATGCAGCAAAAGCAATGATTCCAGCAGGTCAAGGCAGCATAATTATAACTGCAAGTGTTTGTTCAAGCATTGGTGGGATTGGGCCATATGCTTACACAAGTTCAAAGCATGGGTTGTTGGGATTAATGAGGAATGCAAGTATTGATTTGGGAAGATATGGAATAAAGGTGAATTGTGTGTCACCTCATGTAGTCCCAACTCAAATGACAAGAGAACATTTTAAGTTGAGAGATGGTGATGAGTTTCCTGATGTTTATTCTCATAATTTCAAATGTGGAGATATTTTAAGGAAAGAAGATGTAGCTGAAGTTGGTTTGTATTTAGCAAGTGATGCATCTAGATTTGTTAGTGGACATAACTTTGTTCTTGATGGAGGCTTCACTGCAGGAAACCAAGGTCTTTGCTCCTATCAACAATTTGGTAACTGA

mRNA sequence

AGACATCACTATTCCAGATATATATTTAAAGAGTTTATATAGTAATTGTATTTGAGAACAAGTAATATTACTTTTGGACAAAGAAGCAGCAAGCCATAGCCATGGGCGTCCCCTTGCTTCCAGCTGCTGCAAGAAGACTAGAAGGCAAAGTGGCAATAATCACAGGTGGAGCAAGAGGAATAGGAGAGTCCATTGCAAAACACTTCTTTAAGCATGGAGCAAAAGTTGTAATTGCAGACATCCTAGACGATTTAGGTAACTCTCTTTCCAACCATCTCTCCTCTTCATCTACTTCCTTCGTCCACTGCAACGTAACGAAAGAAACCGACGTCGAAAATGTCGTTAACACTGCCGTTTCCAAATACGGAAAGCTAGACATCATGTTCAACAACGCAGGCATTCCAGGAGCTCTCAAGTTCAACATACTCGAAAACGAATATTCAGACTTCCAAAACGTACTCAATGTCAACCTAGTTGGAGCCTTTTTAGGCACAAAACATGCAGCAAAAGCAATGATTCCAGCAGGTCAAGGCAGCATAATTATAACTGCAAGTGTTTGTTCAAGCATTGGTGGGATTGGGCCATATGCTTACACAAGTTCAAAGCATGGGTTGTTGGGATTAATGAGGAATGCAAGTATTGATTTGGGAAGATATGGAATAAAGGTGAATTGTGTGTCACCTCATGTAGTCCCAACTCAAATGACAAGAGAACATTTTAAGTTGAGAGATGGTGATGAGTTTCCTGATGTTTATTCTCATAATTTCAAATGTGGAGATATTTTAAGGAAAGAAGATGTAGCTGAAGTTGGTTTGTATTTAGCAAGTGATGCATCTAGATTTGTTAGTGGACATAACTTTGTTCTTGATGGAGGCTTCACTGCAGGAAACCAAGGTCTTTGCTCCTATCAACAATTTGGTAACTGA

Coding sequence (CDS)

ATGGGCGTCCCCTTGCTTCCAGCTGCTGCAAGAAGACTAGAAGGCAAAGTGGCAATAATCACAGGTGGAGCAAGAGGAATAGGAGAGTCCATTGCAAAACACTTCTTTAAGCATGGAGCAAAAGTTGTAATTGCAGACATCCTAGACGATTTAGGTAACTCTCTTTCCAACCATCTCTCCTCTTCATCTACTTCCTTCGTCCACTGCAACGTAACGAAAGAAACCGACGTCGAAAATGTCGTTAACACTGCCGTTTCCAAATACGGAAAGCTAGACATCATGTTCAACAACGCAGGCATTCCAGGAGCTCTCAAGTTCAACATACTCGAAAACGAATATTCAGACTTCCAAAACGTACTCAATGTCAACCTAGTTGGAGCCTTTTTAGGCACAAAACATGCAGCAAAAGCAATGATTCCAGCAGGTCAAGGCAGCATAATTATAACTGCAAGTGTTTGTTCAAGCATTGGTGGGATTGGGCCATATGCTTACACAAGTTCAAAGCATGGGTTGTTGGGATTAATGAGGAATGCAAGTATTGATTTGGGAAGATATGGAATAAAGGTGAATTGTGTGTCACCTCATGTAGTCCCAACTCAAATGACAAGAGAACATTTTAAGTTGAGAGATGGTGATGAGTTTCCTGATGTTTATTCTCATAATTTCAAATGTGGAGATATTTTAAGGAAAGAAGATGTAGCTGAAGTTGGTTTGTATTTAGCAAGTGATGCATCTAGATTTGTTAGTGGACATAACTTTGTTCTTGATGGAGGCTTCACTGCAGGAAACCAAGGTCTTTGCTCCTATCAACAATTTGGTAACTGA

Protein sequence

MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN*

Homology

[Close]

BLAST of CSPI01G15040 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 9.4e-70
Identity = 134/268 (50.00%), Postives = 192/268 (71.64%), Query Frame = 0


Query: 5   LLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSST 64
           +L A ARRLEGKVA+ITGGA GIGE+ AK F +HGAKV IAD+ D+LG+S+   + +S++
Sbjct: 7   VLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNS 66

Query: 65  SFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNL 124
           +++HC+VT E  V+N V+  VS YGKLDIMF+NAGI    +  I++NE +DF+ V +VN+
Sbjct: 67  TYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNV 126

Query: 125 VGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLGR 184
            G FL  KHAA+ MIPA  G+II TAS+ S++GG   +AY  SKH +LGL RN +++LG+
Sbjct: 127 TGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQ 186

Query: 185 YGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDV--YSHNFKCGDILRKEDVAEVGLYLAS 244
           +GI+VNC+SP  +PT + ++   +++ +EF +V  ++ N K G     EDVA   LYLAS
Sbjct: 187 FGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLK-GPKFNVEDVANAALYLAS 246

Query: 245 DASRFVSGHNFVLDGGFTAGNQGLCSYQ 271
           D +++VSGHN  +DGGF+  N  +  +Q
Sbjct: 247 DEAKYVSGHNLFIDGGFSVCNSVIKVFQ 273

[Close]

BLAST of CSPI01G15040 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 9.1e-65
Identity = 134/257 (52.14%), Postives = 172/257 (66.93%), Query Frame = 0


Query: 8   AAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSSTSFV 67
           A AR+L GKVA+ITGGA GIG   A+ F KHGA+VV+ADI D+LG SL   L   ++S+V
Sbjct: 10  ADARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYV 69

Query: 68  HCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGA 127
           HC+VT E DV   V+ AV+++GKLD+MFNNAG+ G   F + E    DF+ VL VNLVG 
Sbjct: 70  HCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGP 129

Query: 128 FLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLGRYGI 187
           FLGTKHAA+ M PA +GSII TAS+ SS+ G   +AYT+SKH L+G   NA+ +LGR+GI
Sbjct: 130 FLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGI 189

Query: 188 KVNCVSPHVVPTQMTREHFKLRD-GDEFPDVYSHNFKCGDILRKEDVAEVGLYLASDASR 247
           +VNCVSP  V T + R    + D   E     S N K    L+ +D+A   L+LASD  R
Sbjct: 190 RVNCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGR 249

Query: 248 FVSGHNFVLDGGFTAGN 264
           +VSG N  +DGG +  N
Sbjct: 250 YVSGQNLRVDGGLSVVN 266

[Close]

BLAST of CSPI01G15040 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.2e-62
Identity = 132/266 (49.62%), Postives = 179/266 (67.29%), Query Frame = 0


Query: 11  RRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSSTS----F 70
           +RLEGKVAIITGGA GIG++    F +HGA VVIAD+ +  G+SL+  LSS  TS    F
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 71  VHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALK--FNILENEYSDFQNVLNVNL 130
           + C+V+ E DVEN+VN  V++YG+LDI+FNNAG+ G  K   +IL+ +  +F +V+ VN+
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 131 VGAFLGTKHAAKAMIPAG-QGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLG 190
            G  LG KH A+AMI  G +G II TASV   +GG+GP+AYT+SKH ++GL +NA+ +LG
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 191 RYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPD---------VYSHNFKCGDILRKEDVA 250
           +YGI+VNC+SP  V T M    ++   G +  D         V S     G+ LR  D+A
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIA 269

Query: 251 EVGLYLASDASRFVSGHNFVLDGGFT 261
           E  LYLASD S++V+GHN V+DGG T
Sbjct: 270 EAALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of CSPI01G15040 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.2e-62
Identity = 128/260 (49.23%), Postives = 178/260 (68.46%), Query Frame = 0


Query: 8   AAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSST-SF 67
           ++  RL+ KVAIITGGA GIGE+ AK F ++GAKVVIADI DD G  + N++ S    SF
Sbjct: 9   SSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISF 68

Query: 68  VHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVG 127
           VHC+VTK+ DV N+V+T ++K+GKLDIMF N G+     ++ILE    DF+ V+++N+ G
Sbjct: 69  VHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYG 128

Query: 128 AFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIG-PYAYTSSKHGLLGLMRNASIDLGRY 187
           AFL  KHAA+ MIPA +GSI+ TAS+ S   G G  + YT++KH +LGL  +   +LG Y
Sbjct: 129 AFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEY 188

Query: 188 GIKVNCVSPHVVPTQMTREHFKLRDGDEFPDV--YSHNFKCGDILRKEDVAEVGLYLASD 247
           GI+VNCVSP++V + +  + F + D     ++   + N K G +LR EDVA+   YLA D
Sbjct: 189 GIRVNCVSPYIVASPLLTDVFGV-DSSRVEELAHQAANLK-GTLLRAEDVADAVAYLAGD 248

Query: 248 ASRFVSGHNFVLDGGFTAGN 264
            S++VSG N V+DGG+T  N
Sbjct: 249 ESKYVSGLNLVIDGGYTRTN 266

[Close]

BLAST of CSPI01G15040 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 9.5e-62
Identity = 132/268 (49.25%), Postives = 183/268 (68.28%), Query Frame = 0


Query: 1   MGVPLLPAAA-RRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHL 60
           MG    PA++  RL+ KVAIITGGA GIGE+ AK F ++GAKVVIADI DD G  + N++
Sbjct: 1   MGSTSTPASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNI 60

Query: 61  SSSST-SFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQN 120
            S    SFVHC+VTK+ DV N+V+T ++K+GKLDIMF N G+     ++ILE    DF+ 
Sbjct: 61  GSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKR 120

Query: 121 VLNVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIG-PYAYTSSKHGLLGLMRN 180
           V+++N+ GAFL  KHAA+ MIPA +GSI+ TAS+ S   G G  + YT++KH +LGL  +
Sbjct: 121 VMDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTS 180

Query: 181 ASIDLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDV--YSHNFKCGDILRKEDVAE 240
              +LG++GI+VNCVSP+VV + +  + F + D     ++   + N K G +LR EDVA+
Sbjct: 181 LCTELGQHGIRVNCVSPYVVASPLLTDVFGV-DSSRVEELAHQAANLK-GILLRAEDVAD 240

Query: 241 VGLYLASDASRFVSGHNFVLDGGFTAGN 264
              YLA D S++VSG N V+DGG+T  N
Sbjct: 241 AVAYLAGDESKYVSGLNLVIDGGYTRTN 266

[Close]

BLAST of CSPI01G15040 vs. ExPASy TrEMBL
Match: A0A0A0LT69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168890 PE=4 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.5e-150
Identity = 268/274 (97.81%), Postives = 271/274 (98.91%), Query Frame = 0


Query: 1   MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLS 60
           MGVPLLPAAARRLEGK AIITGGARGIGESIAKHFF  GAKVVIADILDDLGNSLSNHLS
Sbjct: 1   MGVPLLPAAARRLEGKAAIITGGARGIGESIAKHFFNPGAKVVIADILDDLGNSLSNHLS 60

Query: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120
           SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL
Sbjct: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120

Query: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASI 180
           NVNLVGAFLGTKHAAKAMIPAGQGSI+ITASVCSSIGGIGPYAYTSSKHGLLGLMRNA+I
Sbjct: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIVITASVCSSIGGIGPYAYTSSKHGLLGLMRNATI 180

Query: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240
           DLGRYGIKVNCVSPHVVPTQMTREHFKL+DGDEFPDVYSHNFKCGDILRKEDVAEVGLYL
Sbjct: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLKDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240

Query: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN 275
           ASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN
Sbjct: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN 274

[Close]

BLAST of CSPI01G15040 vs. ExPASy TrEMBL
Match: A0A6J1EI14 (secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 6.9e-124
Identity = 223/270 (82.59%), Postives = 245/270 (90.74%), Query Frame = 0


Query: 1   MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLS 60
           MG+ LLPAAARRLEGKVA+ITG ARGIGES AK FFKHGAKVV+ADILDD+G+SLS HL 
Sbjct: 1   MGISLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120
           SSS+SFVHC+VTKE D+EN VNTA+SKYGKLDIM NNAGI G LKFNILENE SDFQ VL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASI 180
           NVNL+GAFLGTKHAAKAMIPA QGSIII AS CS IGGIGPYAYTSSKHGLLGLM+NA++
Sbjct: 121 NVNLLGAFLGTKHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATV 180

Query: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240
           DLGRYGI+VNCVSPHVVP+QMTR+ FKL DGDEFP+VYSH FKCGDILR ED+AE  LYL
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSH-FKCGDILRPEDIAEAALYL 240

Query: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQ 271
           ASDASRFVSGHN ++DGGFT+ NQGLCSY+
Sbjct: 241 ASDASRFVSGHNLIVDGGFTSANQGLCSYE 269

[Close]

BLAST of CSPI01G15040 vs. ExPASy TrEMBL
Match: A0A6J1I6S5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471670 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.2e-123
Identity = 221/270 (81.85%), Postives = 245/270 (90.74%), Query Frame = 0


Query: 1   MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLS 60
           MGV LLPAAARRLEGKVA+ITG ARGIGES AK FFKHGAKVV+ADILDD+G+SLS HL 
Sbjct: 1   MGVSLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120
           SSS+SFVHC+VTKE D+EN VNTA++KYGKLDIM NNAGI G LKFN LENE SDFQNVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVL 120

Query: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASI 180
           NVNL+G FLGTKHAA+AMIPA QGSII+TASVCS IGGIGPY YTSSKHG+LGLM+NA++
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240
           DLGRYGI+VNCVSPHVVP+QMTR+ FKL DGDEFP+VYSH FKCGDILR ED+AE  LYL
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSH-FKCGDILRAEDIAEAALYL 240

Query: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQ 271
           ASDASRFVSGHN ++DGGFT+ NQGLCSYQ
Sbjct: 241 ASDASRFVSGHNLIVDGGFTSANQGLCSYQ 269

[Close]

BLAST of CSPI01G15040 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.0e-123
Identity = 222/270 (82.22%), Postives = 244/270 (90.37%), Query Frame = 0


Query: 1   MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLS 60
           MG+ LLP AARRLEGKVA+ITG ARGIGES AK FFKHGAKVV+ADILDD+G+SLS HL 
Sbjct: 1   MGISLLPVAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120
           SSS+SFVHC+VTKE D+EN VNTA+SKYGKLDIM NNAGI G LKFNILENE SDFQ VL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASI 180
           NVNL+GAFLGTKHAAKAMIPA QGSIII AS CS IGGIGPYAYTSSKHGLLGLM+NA++
Sbjct: 121 NVNLLGAFLGTKHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATV 180

Query: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240
           DLGRYGI+VNCVSPHVVP+QMTR+ FKL DGDEFP+VYSH FKCGDILR ED+AE  LYL
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSH-FKCGDILRPEDIAEAALYL 240

Query: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQ 271
           ASDASRFVSGHN ++DGGFT+ NQGLCSY+
Sbjct: 241 ASDASRFVSGHNLIVDGGFTSANQGLCSYE 269

[Close]

BLAST of CSPI01G15040 vs. ExPASy TrEMBL
Match: A0A6J1EPB9 (secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.5e-123
Identity = 223/270 (82.59%), Postives = 244/270 (90.37%), Query Frame = 0


Query: 1   MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLS 60
           MGV LLPAAAR LEGKVA+ITG ARGIGES AK FFKHGAKVV+ADILDD+G+SLS HL 
Sbjct: 1   MGVSLLPAAARWLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120
           SSS+SFVHC+VTKE D+EN VNTA+SKYGKLDIM NNAGI G LKFNILENE SDFQ VL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASI 180
           NVNL+GAFLGTKHAAKAMIPA QGSIII AS CS IGGIGPYAYTSSKHGLLGLM+NA++
Sbjct: 121 NVNLLGAFLGTKHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATV 180

Query: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240
           DLGRYGI+VNCVSPHVVP+QMTR+ FKL DGDEFP+VYSH FKCGDILR ED+AE  LYL
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSH-FKCGDILRPEDIAEAALYL 240

Query: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQ 271
           ASDASRFVSGHN ++DGGFT+ NQGLCSY+
Sbjct: 241 ASDASRFVSGHNLIVDGGFTSANQGLCSYE 269

[Close]

BLAST of CSPI01G15040 vs. NCBI nr
Match: KAE8637559.1 (hypothetical protein CSA_004626 [Cucumis sativus])

HSP 1 Score: 536.6 bits (1381), Expect = 1.3e-148
Identity = 266/273 (97.44%), Postives = 269/273 (98.53%), Query Frame = 0


Query: 2   GVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSS 61
           G PLLPAAARRLEGK AIITGGARGIGESIAKHFF  GAKVVIADILDDLGNSLSNHLSS
Sbjct: 38  GRPLLPAAARRLEGKAAIITGGARGIGESIAKHFFNPGAKVVIADILDDLGNSLSNHLSS 97

Query: 62  SSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLN 121
           SSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLN
Sbjct: 98  SSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLN 157

Query: 122 VNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASID 181
           VNLVGAFLGTKHAAKAMIPAGQGSI+ITASVCSSIGGIGPYAYTSSKHGLLGLMRNA+ID
Sbjct: 158 VNLVGAFLGTKHAAKAMIPAGQGSIVITASVCSSIGGIGPYAYTSSKHGLLGLMRNATID 217

Query: 182 LGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLA 241
           LGRYGIKVNCVSPHVVPTQMTREHFKL+DGDEFPDVYSHNFKCGDILRKEDVAEVGLYLA
Sbjct: 218 LGRYGIKVNCVSPHVVPTQMTREHFKLKDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLA 277

Query: 242 SDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN 275
           SDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN
Sbjct: 278 SDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN 310

[Close]

BLAST of CSPI01G15040 vs. NCBI nr
Match: XP_031739140.1 (secoisolariciresinol dehydrogenase [Cucumis sativus] >XP_031745375.1 secoisolariciresinol dehydrogenase-like [Cucumis sativus])

HSP 1 Score: 522.3 bits (1344), Expect = 2.5e-144
Identity = 262/276 (94.93%), Postives = 265/276 (96.01%), Query Frame = 0


Query: 1   MGVPL--LPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNH 60
           MGVP   L     RLEGK AIITGGARGIGESIAKHFF  GAKVVIADILDDLGNSLSNH
Sbjct: 1   MGVPCFQLLQEDNRLEGKAAIITGGARGIGESIAKHFFNPGAKVVIADILDDLGNSLSNH 60

Query: 61  LSSSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQN 120
           LSSSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQN
Sbjct: 61  LSSSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQN 120

Query: 121 VLNVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNA 180
           VLNVNLVGAFLGTKHAAKAMIPAGQGSI+ITASVCSSIGGIGPYAYTSSKHGLLGLMRNA
Sbjct: 121 VLNVNLVGAFLGTKHAAKAMIPAGQGSIVITASVCSSIGGIGPYAYTSSKHGLLGLMRNA 180

Query: 181 SIDLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGL 240
           +IDLGRYGIKVNCVSPHVVPTQMTREHFKL+DGDEFPDVYSHNFKCGDILRKEDVAEVGL
Sbjct: 181 TIDLGRYGIKVNCVSPHVVPTQMTREHFKLKDGDEFPDVYSHNFKCGDILRKEDVAEVGL 240

Query: 241 YLASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN 275
           YLASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN
Sbjct: 241 YLASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFGN 276

[Close]

BLAST of CSPI01G15040 vs. NCBI nr
Match: KAE8652818.1 (hypothetical protein Csa_022860 [Cucumis sativus])

HSP 1 Score: 516.2 bits (1328), Expect = 1.8e-142
Identity = 258/273 (94.51%), Postives = 261/273 (95.60%), Query Frame = 0


Query: 1   MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLS 60
           MGVP         EGK AIITGGARGIGESIAKHFF  GAKVVIADILDDLGNSLSNHLS
Sbjct: 1   MGVPCFQLLQE--EGKAAIITGGARGIGESIAKHFFNPGAKVVIADILDDLGNSLSNHLS 60

Query: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120
           SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL
Sbjct: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120

Query: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASI 180
           NVNLVGAFLGTKHAAKAMIPAGQGSI+ITASVCSSIGGIGPYAYTSSKHGLLGLMRNA+I
Sbjct: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIVITASVCSSIGGIGPYAYTSSKHGLLGLMRNATI 180

Query: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240
           DLGRYGIKVNCVSPHVVPTQMTREHFKL+DGDEFPDVYSHNFKCGDILRKEDVAEVGLYL
Sbjct: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLKDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240

Query: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFG 274
           ASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFG
Sbjct: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQQFG 271

[Close]

BLAST of CSPI01G15040 vs. NCBI nr
Match: XP_022927566.1 (secoisolariciresinol dehydrogenase-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 453.4 bits (1165), Expect = 1.4e-123
Identity = 223/270 (82.59%), Postives = 245/270 (90.74%), Query Frame = 0


Query: 1   MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLS 60
           MG+ LLPAAARRLEGKVA+ITG ARGIGES AK FFKHGAKVV+ADILDD+G+SLS HL 
Sbjct: 1   MGISLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120
           SSS+SFVHC+VTKE D+EN VNTA+SKYGKLDIM NNAGI G LKFNILENE SDFQ VL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASI 180
           NVNL+GAFLGTKHAAKAMIPA QGSIII AS CS IGGIGPYAYTSSKHGLLGLM+NA++
Sbjct: 121 NVNLLGAFLGTKHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATV 180

Query: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240
           DLGRYGI+VNCVSPHVVP+QMTR+ FKL DGDEFP+VYSH FKCGDILR ED+AE  LYL
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSH-FKCGDILRPEDIAEAALYL 240

Query: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQ 271
           ASDASRFVSGHN ++DGGFT+ NQGLCSY+
Sbjct: 241 ASDASRFVSGHNLIVDGGFTSANQGLCSYE 269

[Close]

BLAST of CSPI01G15040 vs. NCBI nr
Match: XP_022973162.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 452.6 bits (1163), Expect = 2.4e-123
Identity = 221/270 (81.85%), Postives = 245/270 (90.74%), Query Frame = 0


Query: 1   MGVPLLPAAARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLS 60
           MGV LLPAAARRLEGKVA+ITG ARGIGES AK FFKHGAKVV+ADILDD+G+SLS HL 
Sbjct: 1   MGVSLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  SSSTSFVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVL 120
           SSS+SFVHC+VTKE D+EN VNTA++KYGKLDIM NNAGI G LKFN LENE SDFQNVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVL 120

Query: 121 NVNLVGAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASI 180
           NVNL+G FLGTKHAA+AMIPA QGSII+TASVCS IGGIGPY YTSSKHG+LGLM+NA++
Sbjct: 121 NVNLLGTFLGTKHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATV 180

Query: 181 DLGRYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYL 240
           DLGRYGI+VNCVSPHVVP+QMTR+ FKL DGDEFP+VYSH FKCGDILR ED+AE  LYL
Sbjct: 181 DLGRYGIRVNCVSPHVVPSQMTRDLFKLEDGDEFPNVYSH-FKCGDILRAEDIAEAALYL 240

Query: 241 ASDASRFVSGHNFVLDGGFTAGNQGLCSYQ 271
           ASDASRFVSGHN ++DGGFT+ NQGLCSYQ
Sbjct: 241 ASDASRFVSGHNLIVDGGFTSANQGLCSYQ 269

[Close]

BLAST of CSPI01G15040 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 239.6 bits (610), Expect = 3.0e-63
Identity = 132/266 (49.62%), Postives = 179/266 (67.29%), Query Frame = 0


Query: 11  RRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSSTS----F 70
           +RLEGKVAIITGGA GIG++    F +HGA VVIAD+ +  G+SL+  LSS  TS    F
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 71  VHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALK--FNILENEYSDFQNVLNVNL 130
           + C+V+ E DVEN+VN  V++YG+LDI+FNNAG+ G  K   +IL+ +  +F +V+ VN+
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 131 VGAFLGTKHAAKAMIPAG-QGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLG 190
            G  LG KH A+AMI  G +G II TASV   +GG+GP+AYT+SKH ++GL +NA+ +LG
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 191 RYGIKVNCVSPHVVPTQMTREHFKLRDGDEFPD---------VYSHNFKCGDILRKEDVA 250
           +YGI+VNC+SP  V T M    ++   G +  D         V S     G+ LR  D+A
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIA 269

Query: 251 EVGLYLASDASRFVSGHNFVLDGGFT 261
           E  LYLASD S++V+GHN V+DGG T
Sbjct: 270 EAALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of CSPI01G15040 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 7.7e-59
Identity = 122/264 (46.21%), Postives = 165/264 (62.50%), Query Frame = 0


Query: 10  ARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHL----SSSSTS 69
           ++RL GKVA+ITGGA GIGESI + F KHGAKV I D+ DDLG  +   L    S  +  
Sbjct: 15  SQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAF 74

Query: 70  FVHCNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLV 129
           F+H +V  E D+ N V+ AV  +G LDI+ NNAG+ GA   +I     S+F+   +VN+ 
Sbjct: 75  FIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVK 134

Query: 130 GAFLGTKHAAKAMIPAGQGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLGRY 189
           GAFL  KHAA+ MIP  +GSI+   SV   +GG+GP++Y  SKH +LGL R+ + +LG++
Sbjct: 135 GAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQH 194

Query: 190 GIKVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKC------GDILRKEDVAEVGLY 249
           GI+VNCVSP+ V T++   H    +  E   V   NF        G  L  +DVA   L+
Sbjct: 195 GIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVANAVLF 254

Query: 250 LASDASRFVSGHNFVLDGGFTAGN 264
           LASD SR++SG N ++DGGFT  N
Sbjct: 255 LASDDSRYISGDNLMIDGGFTCTN 278

[Close]

BLAST of CSPI01G15040 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 216.1 bits (549), Expect = 3.6e-56
Identity = 118/252 (46.83%), Postives = 169/252 (67.06%), Query Frame = 0


Query: 12  RLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSSTSFVHCNV 71
           RL+GK+AIITGGA GIG    + F  HGAKVVI DI ++LG +L+  +     SF  CNV
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 72  TKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGAFLGT 131
           T ETDVEN V   V K+GKLD++F+NAG+  A   ++L+ +   F   + VN+ GA    
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAAFI 124

Query: 132 KHAAKAMIPAG-QGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLGRYGIKVN 191
           KHAA++M+ +G +GSI+ T S+ + IGG GP++YT+SKH LLGL+R+A   LG+YGI+VN
Sbjct: 125 KHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVN 184

Query: 192 CVSPHVVPTQMTREHFK--LRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLASDASRFV 251
            V+P+ V T MT  + +  ++  +E+ +    N K G +L+   +AE  L+LASD S ++
Sbjct: 185 GVAPYGVATGMTSAYNEEAVKMLEEYGEALG-NLK-GVVLKARHIAEAALFLASDDSVYI 244

Query: 252 SGHNFVLDGGFT 261
           SG N V+DGGF+
Sbjct: 245 SGQNLVVDGGFS 253

[Close]

BLAST of CSPI01G15040 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 213.8 bits (543), Expect = 1.8e-55
Identity = 117/251 (46.61%), Postives = 168/251 (66.93%), Query Frame = 0


Query: 13  LEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSSTSFVHCNVT 72
           L+GK+AIITGGA GIG    + F  HGAKVVI DI ++LG +L+  +     SF  CNVT
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNVT 103

Query: 73  KETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGAFLGTK 132
            ETDVEN V   V K+GKLD++F+NAG+  A   ++L+ +   F   + VN+ GA    K
Sbjct: 104 DETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAAFIK 163

Query: 133 HAAKAMIPAG-QGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLGRYGIKVNC 192
           HAA++M+ +G +GSI+ T S+ + IGG GP++YT+SKH LLGL+R+A   LG+YGI+VN 
Sbjct: 164 HAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVNG 223

Query: 193 VSPHVVPTQMTREHFK--LRDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLASDASRFVS 252
           V+P+ V T MT  + +  ++  +E+ +    N K G +L+   +AE  L+LASD S ++S
Sbjct: 224 VAPYGVATGMTSAYNEEAVKMLEEYGEALG-NLK-GVVLKARHIAEAALFLASDDSVYIS 283

Query: 253 GHNFVLDGGFT 261
           G N V+DGGF+
Sbjct: 284 GQNLVVDGGFS 291

[Close]

BLAST of CSPI01G15040 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 3.9e-55
Identity = 113/258 (43.80%), Postives = 162/258 (62.79%), Query Frame = 0


Query: 9   AARRLEGKVAIITGGARGIGESIAKHFFKHGAKVVIADILDDLGNSLSNHLSSSSTSFVH 68
           + +RL+GK+ IITGGA GIG    + F +HGA+VVI D+ D+LG +++  +     S+ H
Sbjct: 2   SGKRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYH 61

Query: 69  CNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGAF 128
           C+VT ET+VEN V   V KYGKLD++F+NAG+      +IL+   ++    + +NL G  
Sbjct: 62  CDVTNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNELDRTIAINLRGTA 121

Query: 129 LGTKHAAKAMIPAG-QGSIIITASVCSSIGGIGPYAYTSSKHGLLGLMRNASIDLGRYGI 188
              KHAA+AM+  G +GSI+ T SV + I G  P+ YT+SKHGLLGL+++AS  LG+YGI
Sbjct: 122 AFIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGI 181

Query: 189 KVNCVSPHVVPTQMTREHFKLRDGDEFPDVYSHNFKC-----GDILRKEDVAEVGLYLAS 248
           +VN V+P  V T +    FK+      P+V   N        G +L+   VAE  L+LAS
Sbjct: 182 RVNGVAPFGVATPLVCNGFKME-----PNVVEQNTSASANLKGIVLKARHVAEAALFLAS 241

Query: 249 DASRFVSGHNFVLDGGFT 261
           D S +VSG N  +DGG++
Sbjct: 242 DESAYVSGQNLAVDGGYS 253

The following BLAST results are available for this feature:

BLAST of CSPI01G15040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	9.4e-70	50.00	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	9.1e-65	52.14	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
Q9SCU0	4.2e-62	49.62	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]
Q94KL8	4.2e-62	49.23	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	9.5e-62	49.25	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]

BLAST of CSPI01G15040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LT69	2.5e-150	97.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168890 PE=4 SV=1	[more]
A0A6J1EI14	6.9e-124	82.59	secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1I6S5	1.2e-123	81.85	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]
A0A6J1EID0	2.0e-123	82.22	secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EPB9	4.5e-123	82.59	secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]

BLAST of CSPI01G15040 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8637559.1	1.3e-148	97.44	hypothetical protein CSA_004626 [Cucumis sativus]	[more]
XP_031739140.1	2.5e-144	94.93	secoisolariciresinol dehydrogenase [Cucumis sativus] >XP_031745375.1 secoisolari...	[more]
KAE8652818.1	1.8e-142	94.51	hypothetical protein Csa_022860 [Cucumis sativus]	[more]
XP_022927566.1	1.4e-123	82.59	secoisolariciresinol dehydrogenase-like isoform X3 [Cucurbita moschata]	[more]
XP_022973162.1	2.4e-123	81.85	secoisolariciresinol dehydrogenase-like [Cucurbita maxima]	[more]

BLAST of CSPI01G15040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	3.0e-63	49.62	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	7.7e-59	46.21	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.2	3.6e-56	46.83	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.1	1.8e-55	46.61	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	3.9e-55	43.80	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 89..100 score: 51.1 coord: 144..152 score: 45.92 coord: 164..183 score: 26.97
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 164..183 score: 31.78 coord: 223..243 score: 29.61 coord: 185..202 score: 38.72 coord: 89..100 score: 58.12 coord: 138..154 score: 32.13 coord: 17..34 score: 48.87
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 22..260 e-value: 8.4E-62 score: 208.8
None	No IPR available	GENE3D	3.40.50.720		coord: 7..271 e-value: 1.0E-73 score: 249.7
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 13..260 e-value: 1.7E-17 score: 61.3
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 19..224 e-value: 1.6E-14 score: 51.6
None	No IPR available	PANTHER	PTHR43180:SF30	MOMILACTONE A SYNTHASE	coord: 8..265
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 8..265
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 14..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G15040.1	CSPI01G15040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity