CSPI01G11700 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI01G11700
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Aspergillus nuclease S(1)
Location	Chr1: 7348816 .. 7351493 (+)
RNA-Seq Expression	CSPI01G11700
Synteny	CSPI01G11700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATATATATATATATATAGATACATATAGGCTTGTGCAAACATTTCTTTTCAACTCAACAAAATGGGAAAATTGGTATTTTTGGTGGTGTTAATTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGTAACTTTATTTACTTTTTCTTGTCTGATGATCTTTTAATTAAATTGGACGTTTTGGATCAAATTCTGCCTTGACCATTTGAACTCAACACATAGGGTCATTTTTACTCTTAAATTTGACAAACTGTCTCCATTTTTATCTGCAATTTTCTATTTTCTTTAATCAGTTTTTATATAAGCTTAAAAAAAATTGTTATATGGGTGGGATCTGAGGAGTTTGTTATGAAATCAACAAATAAAACAAGCATAAAAACGTAAATGAGGAGAGAATCGACATAGAAGCATATTAGCTACATTTATGCTCATTAAAAAGAATATTTCAGATTACAGAACAAAGACGCTTCTATAAATGGGGAGTTCATACAACCAATTTGATCTTCAAATCTTAAGATTAAAATGGTAAACAACGTAAATACAGTGAGATCTTAAACATTTTTGTTTAATTAGTTGTTGAGAGGTGAGATATCAGATCTAAATGTATTCACCTCAGTTATAAAATATATATCAGGCAAGAAACTGATGTGTTTTGAAAGAGAAATTAACAACAAAACAGATTTTGGGCTGAATTTTGGAAGATTTATCAGACAATTGACATTGTAAATTGATGTAGCTCAATTCGATCATGTCAAACTTTATTTTTGTTGAAAGCAGCGCGAGTCTTAAAAAGGAGGATTTTACATGTTAAACAAATTTAGAAACAAAAATTTTAAAATTTGAGACGCACAATTGAATATTGAAGAGGACAACTAATGGGTAAGTTTAGATTATAATCCAACCACATTCAGGAGCTACTGATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATCTATCGGCGATGTGCGTATGGCCGGACCAAATCCGACTCCAGTCTAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGTAAAGTAACACCCTTTTATTCTCCCATACCTAAGGTTAAATAAATTCATTTCGAATTATTAAACTAAAAGGTGCGTTTTTGTTTAGGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACCGAACACAAGGCTTCGACAGTCCTCGTAACTGACCTGACCCGACCCACTCGAATTATTCCTGTGATAATATGAAAATTATTTAATTTCATTTTTATTATTCTAGATAACTTGACCGAGGCCTTGCTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGGTAATACTAAAGTTATTCAAAAACGTTTATTTGTTGAATTGCTTTAATTTAATTATGAGATATGTAATTATAACTTATTTTGCATATACTTATATACCTATAATTTCAGCCATTGCATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACTATAGAAGTACGGTGGTTCCGTCGTAAATCAAACCTCCATCATGTAAGATTTTAATCTAAAAACATACCAAAACAATATGAGTCTATATGTTTCAAGTTTTATGAATTCTATTACATTTAGATTATAAATTACTAATTATATGGAATAATGCAGGTGTGGGATAGAGACATTATTCTTGAAGCTCTAGGAGATTATTACGACAAAGACGGTGGCCTTCTCTTAGATGAACTTAACCGGAATTTAACTCAGGTAATTAATTAATACTTTTTATTTTTTCTTTATTACATTAGTACATTAGTTTATAATATTAGGATATTAGAAGGTTTACTATCTTCTTATCAAAATTCTGTTTTTGTAGGGAATTTGGAGCAATGACGTTTCGGAATGGGAGCGTTGTTCTACTGTTAATTCATGTGTTAATAGGTATTTATAGAAAATGAAATTAGGTTATATTTTGTAAATATTTTTTTGTTTTTTTTTCTTAACCATAGTAACAACTTGTGGATTAGGTGGGCTGATGAGAGTACAGGGTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGGTAAACCCAATCCATCAATCAATCAAACATTGTTTAAATTAATATCATAGTCATTAATTCCTAAAATTATTTGAAATATAACATATGATGAGAGGATAATGAAATCCGTAACTGATGTGATTTGTGTACAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACCAGGATTTGCCTATTCATCTTGATTAATTCCACCATCAACTTTAGGATTTCTTTTTCTTCTTTCTTCTAACTTAATTATGTCAGGAAAATATCTTAGATTTCAAAGGTTACTAGAAATGAAAGCAAATTGTAGTGTTGAATAAATTGGCATTTTAGTTCATTTTACAAAATTTCATCAACAAATTCACATGCTCTTTACATAGATGTAAATGAATTAATCAAAGTAATAATGCAGTACAT

mRNA sequence

TATATATATATATATATAGATACATATAGGCTTGTGCAAACATTTCTTTTCAACTCAACAAAATGGGAAAATTGGTATTTTTGGTGGTGTTAATTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGAGCTACTGATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATCTATCGGCGATGTGCGTATGGCCGGACCAAATCCGACTCCAGTCTAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACCGAACACAAGGCTTCGACAGTCCTCATAACTTGACCGAGGCCTTGCTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGCCATTGCATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACTATAGAAGTACGGTGGTTCCGTCGTAAATCAAACCTCCATCATGTGTGGGATAGAGACATTATTCTTGAAGCTCTAGGAGATTATTACGACAAAGACGGTGGCCTTCTCTTAGATGAACTTAACCGGAATTTAACTCAGGGAATTTGGAGCAATGACGTTTCGGAATGGGAGCGTTGTTCTACTGTTAATTCATGTGTTAATAGGTGGGCTGATGAGAGTACAGGGTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACCAGGATTTGCCTATTCATCTTGATTAATTCCACCATCAACTTTAGGATTTCTTTTTCTTCTTTCTTCTAACTTAATTATGTCAGGAAAATATCTTAGATTTCAAAGGTTACTAGAAATGAAAGCAAATTGTAGTGTTGAATAAATTGGCATTTTAGTTCATTTTACAAAATTTCATCAACAAATTCACATGCTCTTTACATAGATGTAAATGAATTAATCAAAGTAATAATGCAGTACAT

Coding sequence (CDS)

ATGGGAAAATTGGTATTTTTGGTGGTGTTAATTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGAGCTACTGATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATCTATCGGCGATGTGCGTATGGCCGGACCAAATCCGACTCCAGTCTAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACCGAACACAAGGCTTCGACAGTCCTCATAACTTGACCGAGGCCTTGCTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGCCATTGCATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACTATAGAAGTACGGTGGTTCCGTCGTAAATCAAACCTCCATCATGTGTGGGATAGAGACATTATTCTTGAAGCTCTAGGAGATTATTACGACAAAGACGGTGGCCTTCTCTTAGATGAACTTAACCGGAATTTAACTCAGGGAATTTGGAGCAATGACGTTTCGGAATGGGAGCGTTGTTCTACTGTTAATTCATGTGTTAATAGGTGGGCTGATGAGAGTACAGGGTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACCAGGATTTGCCTATTCATCTTGA

Protein sequence

MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAYSS*

Homology

[Close]

BLAST of CSPI01G11700 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.3e-99
Identity = 167/286 (58.39%), Postives = 219/286 (76.57%), Query Frame = 0


Query: 7   LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCV 66
           L++++ I  L  +   + WSKEGHILTC IAQ LL    A  V++LLP+   G+LSA+CV
Sbjct: 11  LILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCV 70

Query: 67  WPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTY 126
           WPDQIR   KYRW S LHY +TPD +CS+ Y RDCH+  G  DMCV GAI+NFT+QL  Y
Sbjct: 71  WPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHY 130

Query: 127 RTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 186
                D  +N+TEALLFLSHF+GDIHQP+HVGF SD GGNTI++RW++ KSNLHHVWDR+
Sbjct: 131 GEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDRE 190

Query: 187 IILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACK 246
           IIL AL + YDK+  LL ++L +N+T G+W +D+S W  C+ + +C +++A ES  LACK
Sbjct: 191 IILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKLACK 250

Query: 247 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAED 292
           W Y+GV++G TLSEEY+++RLPIVM+R+ QGGVRLAM+LNRVF++D
Sbjct: 251 WGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDD 296

[Close]

BLAST of CSPI01G11700 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.3e-85
Identity = 149/283 (52.65%), Postives = 201/283 (71.02%), Query Frame = 0


Query: 7   LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCV 66
           ++++I +  L   P   GW KEGH + C+IAQ  L   AA+AV++LLPESA G+LS++C+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 67  WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYR 126
           W D+++   +Y W+SPLHY NTPD+CS+ Y RDC +++G+   CVAGAI N+TTQL +Y+
Sbjct: 70  WADRVKF--RYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYK 129

Query: 127 T-QGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 186
           T     S +NLTEALLF+SHF+GDIHQPLHV + SD GGNTIEV W+ RK+NLHH+WD +
Sbjct: 130 TAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSN 189

Query: 187 IILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACK 246
           II  A  D Y+     ++D L +N+T   W++ V  WE C+   +C + +A E    AC 
Sbjct: 190 IIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQAACD 249

Query: 247 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVF 289
           WAY+GV  G TL +EY+ SRLPIV +RLAQGGVRLA  LNR+F
Sbjct: 250 WAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]

BLAST of CSPI01G11700 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.8e-80
Identity = 145/268 (54.10%), Postives = 181/268 (67.54%), Query Frame = 0


Query: 25  WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPDQIRLQSKYRWASPLH 84
           W KEGH   C+IA+     E   AV+ LLP+SA G+L+++C WPD+I+   ++RW SPLH
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 85  YANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPH-NLTEALLF 144
           Y +TPD  C++ Y RDCH+     D CV GAI N+T QL +         H NLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 145 LSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLL 204
           LSHF+GDIHQPLHVGF  D GGNTI VRW+RRK+NLHHVWD  II  AL  YY+K   L+
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 205 LDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGLACKWAYEGVEAGITLSEEY 264
           ++ L  NLT   WSNDV  WE C     +C N +A ES  LACK+AY     G TL ++Y
Sbjct: 205 IEALQANLTND-WSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDY 264

Query: 265 YDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           + SRLPIV +RLAQGG+RLA  LNR+F+
Sbjct: 265 FLSRLPIVEKRLAQGGIRLAATLNRIFS 291

[Close]

BLAST of CSPI01G11700 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 5.0e-72
Identity = 138/287 (48.08%), Postives = 178/287 (62.02%), Query Frame = 0


Query: 10  LIFISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESA-GGNLSAMC 69
           L  +S L++     G   W K+GH   C++A+     +   AV+ LLPES  GG L+  C
Sbjct: 3   LWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFC 62

Query: 70  VWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 129
            WPD+I+  S+++W S LHY NTP+  C++ Y RDCH+     D CV GAI N+T QL +
Sbjct: 63  SWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMS 122

Query: 130 YRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 189
                 +  H NLTEALLFLSH++GD+HQPLH GF  D GGNTI V W+  KSNLHHVWD
Sbjct: 123 ASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWD 182

Query: 190 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGL 249
             II  AL  YY+     ++  L   L  G WSNDV  W+ C     +C N +A ES  L
Sbjct: 183 NMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESIDL 242

Query: 250 ACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           ACK+AY     G TL +EY+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 243 ACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFS 288

[Close]

BLAST of CSPI01G11700 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.6e-70
Identity = 136/283 (48.06%), Postives = 175/283 (61.84%), Query Frame = 0


Query: 13  ISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPD 72
           +S L++     G   W   GH   C+IAQ     +   AV+ LLPESA G L+A+C WPD
Sbjct: 10  VSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPD 69

Query: 73  QIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQ 132
           +I+   ++RW S LH+A+TPD  C++ Y RDC       D CV GAI N+T QL +    
Sbjct: 70  EIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQLMSTSEN 129

Query: 133 GFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDII 192
                H NLTEAL+FLSH++GDIHQPLH GF  D GGN I+V W+ +++NLH VWD  II
Sbjct: 130 SQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMII 189

Query: 193 LEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGLACKW 252
             AL  YY+     ++ EL   L  G WSNDV  WE C     +C N +A ES  LACK+
Sbjct: 190 ESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASESIDLACKY 249

Query: 253 AYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           AY    AG TL + Y+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 250 AYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFS 286

[Close]

BLAST of CSPI01G11700 vs. ExPASy TrEMBL
Match: C3VEY2 (Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 PE=2 SV=2)

HSP 1 Score: 626.7 bits (1615), Expect = 5.0e-176
Identity = 299/300 (99.67%), Postives = 299/300 (99.67%), Query Frame = 0


Query: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60
           MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN
Sbjct: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60

Query: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT 120
           LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT
Sbjct: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT 120

Query: 121 QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH 180
           QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH
Sbjct: 121 QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH 180

Query: 181 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 240
           VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST
Sbjct: 181 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 240

Query: 241 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAYSS 300
           GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDAT GFAYSS
Sbjct: 241 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 300

[Close]

BLAST of CSPI01G11700 vs. ExPASy TrEMBL
Match: A0A5D3DSP7 (Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00370 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.5e-161
Identity = 277/302 (91.72%), Postives = 284/302 (94.04%), Query Frame = 0


Query: 1   MGKLV--FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKL   FLVVL FISFLLVLPCAQGWSKEGHILTCEIAQELL PEAA+AVQDLLPESAG
Sbjct: 1   MGKLAFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLNPEAADAVQDLLPESAG 60

Query: 61  GNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMCVW DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADE 240
           HHVWDRDIIL AL DYYDKDGGLLL+EL RNLTQGIWSNDV  WERC  VNSCVN+WA+E
Sbjct: 181 HHVWDRDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEE 240

Query: 241 STGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAY 300
           ST LACKWAYEGVEAGITLSE+Y+DSRLPIVMERLAQGGVRLAMLLNRVF+EDAT GFAY
Sbjct: 241 STDLACKWAYEGVEAGITLSEDYFDSRLPIVMERLAQGGVRLAMLLNRVFSEDATQGFAY 300

[Close]

BLAST of CSPI01G11700 vs. ExPASy TrEMBL
Match: A0A1S3B4B0 (Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103486022 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.5e-161
Identity = 277/302 (91.72%), Postives = 284/302 (94.04%), Query Frame = 0


Query: 1   MGKLV--FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKL   FLVVL FISFLLVLPCAQGWSKEGHILTCEIAQELL PEAA+AVQDLLPESAG
Sbjct: 1   MGKLAFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLNPEAADAVQDLLPESAG 60

Query: 61  GNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMCVW DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADE 240
           HHVWDRDIIL AL DYYDKDGGLLL+EL RNLTQGIWSNDV  WERC  VNSCVN+WA+E
Sbjct: 181 HHVWDRDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEE 240

Query: 241 STGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAY 300
           ST LACKWAYEGVEAGITLSE+Y+DSRLPIVMERLAQGGVRLAMLLNRVF+EDAT GFAY
Sbjct: 241 STDLACKWAYEGVEAGITLSEDYFDSRLPIVMERLAQGGVRLAMLLNRVFSEDATQGFAY 300

[Close]

BLAST of CSPI01G11700 vs. ExPASy TrEMBL
Match: A0A0A0LUG7 (Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_1G072450 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.4e-140
Identity = 236/237 (99.58%), Postives = 236/237 (99.58%), Query Frame = 0


Query: 64  MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 123
           MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60

Query: 124 TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 183
           TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD
Sbjct: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120

Query: 184 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 243
           RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA
Sbjct: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180

Query: 244 CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAYSS 301
           CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDAT GFAYSS
Sbjct: 181 CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 237

[Close]

BLAST of CSPI01G11700 vs. ExPASy TrEMBL
Match: A0A6J1KL07 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111495151 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.1e-129
Identity = 226/295 (76.61%), Postives = 251/295 (85.08%), Query Frame = 0


Query: 6   FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMC 65
           FLV+L F    L+LP AQGWSKEGH+LTC+IAQELL PEA EAVQ LLPESAGGNLSAMC
Sbjct: 6   FLVLLCFT--FLLLPSAQGWSKEGHVLTCQIAQELLNPEATEAVQALLPESAGGNLSAMC 65

Query: 66  VWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 125
           VW DQIR  SKYRW SPLHY NTPD +CSF+YKRDCHN A Q +MCVAGAIRNFTTQLT 
Sbjct: 66  VWADQIRRWSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTTQLTA 125

Query: 126 YRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDR 185
           +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHVWDR
Sbjct: 126 FPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHVWDR 185

Query: 186 DIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLAC 245
           +IIL AL DYYDKD GLLL++L RNLT GIWS++V  WERC  VNSC+N WA+ES  LAC
Sbjct: 186 EIILTALADYYDKDTGLLLEDLQRNLTHGIWSDNVPTWERCVNVNSCINNWAEESIKLAC 245

Query: 246 KWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAYS 300
            WAYEGVEAG+TLSE+Y+DSRLPIVMERLA+GGVRLAMLLNRVF+E+   GF  S
Sbjct: 246 TWAYEGVEAGMTLSEDYFDSRLPIVMERLAKGGVRLAMLLNRVFSENPKGGFGSS 298

[Close]

BLAST of CSPI01G11700 vs. NCBI nr
Match: NP_001292654.1 (endonuclease 1 precursor [Cucumis sativus] >ACO72982.2 bifunctional nuclease precursor [Cucumis sativus] >KAE8652702.1 hypothetical protein Csa_013303 [Cucumis sativus])

HSP 1 Score: 626.7 bits (1615), Expect = 1.0e-175
Identity = 299/300 (99.67%), Postives = 299/300 (99.67%), Query Frame = 0


Query: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60
           MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN
Sbjct: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60

Query: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT 120
           LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT
Sbjct: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT 120

Query: 121 QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH 180
           QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH
Sbjct: 121 QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH 180

Query: 181 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 240
           VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST
Sbjct: 181 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 240

Query: 241 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAYSS 300
           GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDAT GFAYSS
Sbjct: 241 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 300

[Close]

BLAST of CSPI01G11700 vs. NCBI nr
Match: XP_008442043.1 (PREDICTED: endonuclease 1 [Cucumis melo] >KAA0056948.1 endonuclease 1 [Cucumis melo var. makuwa] >TYK26375.1 endonuclease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 577.4 bits (1487), Expect = 7.2e-161
Identity = 277/302 (91.72%), Postives = 284/302 (94.04%), Query Frame = 0


Query: 1   MGKLV--FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKL   FLVVL FISFLLVLPCAQGWSKEGHILTCEIAQELL PEAA+AVQDLLPESAG
Sbjct: 1   MGKLAFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLNPEAADAVQDLLPESAG 60

Query: 61  GNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMCVW DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADE 240
           HHVWDRDIIL AL DYYDKDGGLLL+EL RNLTQGIWSNDV  WERC  VNSCVN+WA+E
Sbjct: 181 HHVWDRDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEE 240

Query: 241 STGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAY 300
           ST LACKWAYEGVEAGITLSE+Y+DSRLPIVMERLAQGGVRLAMLLNRVF+EDAT GFAY
Sbjct: 241 STDLACKWAYEGVEAGITLSEDYFDSRLPIVMERLAQGGVRLAMLLNRVFSEDATQGFAY 300

[Close]

BLAST of CSPI01G11700 vs. NCBI nr
Match: XP_038895329.1 (LOW QUALITY PROTEIN: endonuclease 1-like [Benincasa hispida])

HSP 1 Score: 501.9 bits (1291), Expect = 3.8e-138
Identity = 243/300 (81.00%), Postives = 260/300 (86.67%), Query Frame = 0


Query: 6   FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMC 65
           FLV+L  ISF LVLPCA GWSKEGH+LTC+IAQELL  EA EAVQDLLPESAGGNLSA+C
Sbjct: 10  FLVLLSSISF-LVLPCAHGWSKEGHVLTCQIAQELLNGEATEAVQDLLPESAGGNLSALC 69

Query: 66  VWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTY 125
           VW DQIRLQSKYRWASPLHYANTPD+CSF+YKRDCHN AGQPDMCVAGAIRNFTTQLTTY
Sbjct: 70  VWADQIRLQSKYRWASPLHYANTPDTCSFLYKRDCHNTAGQPDMCVAGAIRNFTTQLTTY 129

Query: 126 RTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWF-----RRKSNLHH 185
           RTQG DSPHNLTEALLFLSHFVGDIHQPLHVGFESD GGNTIE   F     R++     
Sbjct: 130 RTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFESDEGGNTIESESFEIFNKRKRKXTTQ 189

Query: 186 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 245
           VWDRDIIL A+ DYYDKD GLLL+EL RNLT GIWSNDV  WE C  VNSCVN+WA+ES 
Sbjct: 190 VWDRDIILTAVADYYDKDTGLLLEELQRNLTNGIWSNDVPAWESCVKVNSCVNKWAEESI 249

Query: 246 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAYSS 301
            LACKWAYEGVEAG+TLS++Y+DSRLPIV ERLAQGGVRLAMLLNRVF+ED T GFA SS
Sbjct: 250 DLACKWAYEGVEAGMTLSDDYFDSRLPIVTERLAQGGVRLAMLLNRVFSEDTTRGFASSS 308

[Close]

BLAST of CSPI01G11700 vs. NCBI nr
Match: XP_023000814.1 (endonuclease 1 [Cucurbita maxima])

HSP 1 Score: 471.9 bits (1213), Expect = 4.2e-129
Identity = 226/295 (76.61%), Postives = 251/295 (85.08%), Query Frame = 0


Query: 6   FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMC 65
           FLV+L F    L+LP AQGWSKEGH+LTC+IAQELL PEA EAVQ LLPESAGGNLSAMC
Sbjct: 6   FLVLLCFT--FLLLPSAQGWSKEGHVLTCQIAQELLNPEATEAVQALLPESAGGNLSAMC 65

Query: 66  VWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 125
           VW DQIR  SKYRW SPLHY NTPD +CSF+YKRDCHN A Q +MCVAGAIRNFTTQLT 
Sbjct: 66  VWADQIRRWSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTTQLTA 125

Query: 126 YRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDR 185
           +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHVWDR
Sbjct: 126 FPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHVWDR 185

Query: 186 DIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLAC 245
           +IIL AL DYYDKD GLLL++L RNLT GIWS++V  WERC  VNSC+N WA+ES  LAC
Sbjct: 186 EIILTALADYYDKDTGLLLEDLQRNLTHGIWSDNVPTWERCVNVNSCINNWAEESIKLAC 245

Query: 246 KWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAYS 300
            WAYEGVEAG+TLSE+Y+DSRLPIVMERLA+GGVRLAMLLNRVF+E+   GF  S
Sbjct: 246 TWAYEGVEAGMTLSEDYFDSRLPIVMERLAKGGVRLAMLLNRVFSENPKGGFGSS 298

[Close]

BLAST of CSPI01G11700 vs. NCBI nr
Match: KAG6584021.1 (Endonuclease 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019639.1 Endonuclease 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 471.5 bits (1212), Expect = 5.5e-129
Identity = 226/295 (76.61%), Postives = 250/295 (84.75%), Query Frame = 0


Query: 6   FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMC 65
           FLV+L F    L+LP AQGWSKEGH+LTC+IAQELL PEA EAVQ LLPESAGGNLSAMC
Sbjct: 6   FLVLLCFT--FLLLPSAQGWSKEGHVLTCQIAQELLNPEATEAVQALLPESAGGNLSAMC 65

Query: 66  VWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 125
           VW DQIR  SKYRW SPLHY NTPD +CSF+YKRDCHN A Q +MCVAGAIRNFTTQLT 
Sbjct: 66  VWADQIRRSSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTTQLTA 125

Query: 126 YRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDR 185
           +  QG D+ +NLTEALLFLSHFVGDIHQPLHVGF SD GGNTIE+RW+R KSNLHHVWDR
Sbjct: 126 FPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHHVWDR 185

Query: 186 DIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLAC 245
           +IIL AL DYYDKD  LLL++L RNLT GIWS+DV  WERC  VNSC+N WA+ES  LAC
Sbjct: 186 EIILTALADYYDKDTDLLLEDLQRNLTHGIWSDDVPTWERCVNVNSCINNWAEESIKLAC 245

Query: 246 KWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATPGFAYS 300
            WAYEGVEAG+TLSE+Y+DSRLPIVMERLA+GGVRLAMLLNRVF+E+   GF  S
Sbjct: 246 TWAYEGVEAGMTLSEDYFDSRLPIVMERLAKGGVRLAMLLNRVFSENPKGGFRSS 298

[Close]

BLAST of CSPI01G11700 vs. TAIR 10
Match: AT1G11190.1 (bifunctional nuclease i )

HSP 1 Score: 364.4 bits (934), Expect = 8.9e-101
Identity = 167/286 (58.39%), Postives = 219/286 (76.57%), Query Frame = 0


Query: 7   LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCV 66
           L++++ I  L  +   + WSKEGHILTC IAQ LL    A  V++LLP+   G+LSA+CV
Sbjct: 11  LILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCV 70

Query: 67  WPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTY 126
           WPDQIR   KYRW S LHY +TPD +CS+ Y RDCH+  G  DMCV GAI+NFT+QL  Y
Sbjct: 71  WPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHY 130

Query: 127 RTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 186
                D  +N+TEALLFLSHF+GDIHQP+HVGF SD GGNTI++RW++ KSNLHHVWDR+
Sbjct: 131 GEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDRE 190

Query: 187 IILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACK 246
           IIL AL + YDK+  LL ++L +N+T G+W +D+S W  C+ + +C +++A ES  LACK
Sbjct: 191 IILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKLACK 250

Query: 247 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAED 292
           W Y+GV++G TLSEEY+++RLPIVM+R+ QGGVRLAM+LNRVF++D
Sbjct: 251 WGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDD 296

[Close]

BLAST of CSPI01G11700 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 317.8 bits (813), Expect = 9.5e-87
Identity = 149/283 (52.65%), Postives = 201/283 (71.02%), Query Frame = 0


Query: 7   LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCV 66
           ++++I +  L   P   GW KEGH + C+IAQ  L   AA+AV++LLPESA G+LS++C+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 67  WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYR 126
           W D+++   +Y W+SPLHY NTPD+CS+ Y RDC +++G+   CVAGAI N+TTQL +Y+
Sbjct: 70  WADRVKF--RYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYK 129

Query: 127 T-QGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 186
           T     S +NLTEALLF+SHF+GDIHQPLHV + SD GGNTIEV W+ RK+NLHH+WD +
Sbjct: 130 TAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSN 189

Query: 187 IILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACK 246
           II  A  D Y+     ++D L +N+T   W++ V  WE C+   +C + +A E    AC 
Sbjct: 190 IIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQAACD 249

Query: 247 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVF 289
           WAY+GV  G TL +EY+ SRLPIV +RLAQGGVRLA  LNR+F
Sbjct: 250 WAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]

BLAST of CSPI01G11700 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 299.7 bits (766), Expect = 2.7e-81
Identity = 145/268 (54.10%), Postives = 181/268 (67.54%), Query Frame = 0


Query: 25  WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPDQIRLQSKYRWASPLH 84
           W KEGH   C+IA+     E   AV+ LLP+SA G+L+++C WPD+I+   ++RW SPLH
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 85  YANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPH-NLTEALLF 144
           Y +TPD  C++ Y RDCH+     D CV GAI N+T QL +         H NLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 145 LSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLL 204
           LSHF+GDIHQPLHVGF  D GGNTI VRW+RRK+NLHHVWD  II  AL  YY+K   L+
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 205 LDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGLACKWAYEGVEAGITLSEEY 264
           ++ L  NLT   WSNDV  WE C     +C N +A ES  LACK+AY     G TL ++Y
Sbjct: 205 IEALQANLTND-WSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDY 264

Query: 265 YDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           + SRLPIV +RLAQGG+RLA  LNR+F+
Sbjct: 265 FLSRLPIVEKRLAQGGIRLAATLNRIFS 291

[Close]

BLAST of CSPI01G11700 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 272.7 bits (696), Expect = 3.5e-73
Identity = 138/287 (48.08%), Postives = 178/287 (62.02%), Query Frame = 0


Query: 10  LIFISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESA-GGNLSAMC 69
           L  +S L++     G   W K+GH   C++A+     +   AV+ LLPES  GG L+  C
Sbjct: 3   LWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFC 62

Query: 70  VWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 129
            WPD+I+  S+++W S LHY NTP+  C++ Y RDCH+     D CV GAI N+T QL +
Sbjct: 63  SWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMS 122

Query: 130 YRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 189
                 +  H NLTEALLFLSH++GD+HQPLH GF  D GGNTI V W+  KSNLHHVWD
Sbjct: 123 ASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWD 182

Query: 190 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGL 249
             II  AL  YY+     ++  L   L  G WSNDV  W+ C     +C N +A ES  L
Sbjct: 183 NMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESIDL 242

Query: 250 ACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           ACK+AY     G TL +EY+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 243 ACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFS 288

[Close]

BLAST of CSPI01G11700 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 267.7 bits (683), Expect = 1.1e-71
Identity = 136/283 (48.06%), Postives = 175/283 (61.84%), Query Frame = 0


Query: 13  ISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPD 72
           +S L++     G   W   GH   C+IAQ     +   AV+ LLPESA G L+A+C WPD
Sbjct: 10  VSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPD 69

Query: 73  QIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQ 132
           +I+   ++RW S LH+A+TPD  C++ Y RDC       D CV GAI N+T QL +    
Sbjct: 70  EIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQLMSTSEN 129

Query: 133 GFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDII 192
                H NLTEAL+FLSH++GDIHQPLH GF  D GGN I+V W+ +++NLH VWD  II
Sbjct: 130 SQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMII 189

Query: 193 LEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGLACKW 252
             AL  YY+     ++ EL   L  G WSNDV  WE C     +C N +A ES  LACK+
Sbjct: 190 ESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASESIDLACKY 249

Query: 253 AYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           AY    AG TL + Y+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 250 AYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFS 286

The following BLAST results are available for this feature:

BLAST of CSPI01G11700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXA6	1.3e-99	58.39	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]
Q9C9G4	1.3e-85	52.65	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
F4JJL0	3.8e-80	54.10	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
F4JJL3	5.0e-72	48.08	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q8LDW6	1.6e-70	48.06	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]

BLAST of CSPI01G11700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
C3VEY2	5.0e-176	99.67	Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 PE=2 SV=2	[more]
A0A5D3DSP7	3.5e-161	91.72	Aspergillus nuclease S(1) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3B4B0	3.5e-161	91.72	Aspergillus nuclease S(1) OS=Cucumis melo OX=3656 GN=LOC103486022 PE=3 SV=1	[more]
A0A0A0LUG7	3.4e-140	99.58	Aspergillus nuclease S(1) OS=Cucumis sativus OX=3659 GN=Csa_1G072450 PE=3 SV=1	[more]
A0A6J1KL07	2.1e-129	76.61	Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111495151 PE=3 SV=1	[more]

BLAST of CSPI01G11700 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001292654.1	1.0e-175	99.67	endonuclease 1 precursor [Cucumis sativus] >ACO72982.2 bifunctional nuclease pre...	[more]
XP_008442043.1	7.2e-161	91.72	PREDICTED: endonuclease 1 [Cucumis melo] >KAA0056948.1 endonuclease 1 [Cucumis m...	[more]
XP_038895329.1	3.8e-138	81.00	LOW QUALITY PROTEIN: endonuclease 1-like [Benincasa hispida]	[more]
XP_023000814.1	4.2e-129	76.61	endonuclease 1 [Cucurbita maxima]	[more]
KAG6584021.1	5.5e-129	76.61	Endonuclease 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019639.1 En...	[more]

BLAST of CSPI01G11700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11190.1	8.9e-101	58.39	bifunctional nuclease i	[more]
AT1G68290.1	9.5e-87	52.65	endonuclease 2	[more]
AT4G21585.1	2.7e-81	54.10	endonuclease 4	[more]
AT4G21600.1	3.5e-73	48.08	endonuclease 5	[more]
AT4G21590.1	1.1e-71	48.06	endonuclease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 25..227 e-value: 1.6E-59 score: 201.8
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 5..235
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 25..222 e-value: 8.73515E-66 score: 202.23
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 25..236 e-value: 1.5E-72 score: 246.6
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 25..229
None	No IPR available	PANTHER	PTHR33146:SF14	ENDONUCLEASE 1	coord: 5..235

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G11700.1	CSPI01G11700.1	mRNA
CSPI01G11700.4	CSPI01G11700.4	mRNA
CSPI01G11700.3	CSPI01G11700.3	mRNA
CSPI01G11700.2	CSPI01G11700.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004521	endoribonuclease activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds