CSPI01G11520 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI01G11520
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpurine permease 1
LocationChr1: 7235995 .. 7237373 (+)
RNA-Seq ExpressionCSPI01G11520
SyntenyCSPI01G11520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCAACAACAACAACAAACCACCCCCGCCATGAGAAGGACTCTTCTTGTTTTCAATTGCTTTTTGTTAGCCGTCGGTACCTGCGGTGGTCCTTTGCTTATGCGTTTGTATTTCCTTCACGGCGGAAACCGCGTCTGGCTCTCCACCTTTCTAGAAACCGCCGGATGTCCAATTATTTTCATCCCCTTGCTAATTAGTTACATTCATCGCCGCCGGCGTCGTGCCCACTCCCTTAACCCATCGGAATCTACCAACTCCACCGAGATGATCTTCATGAAGTCACGCCTATTTTTAGCCTCCGGTGTCATCGGCATCATCACCGGCTTCGTGGATTTCTTATACGCTTACGGCGTCGCTCGAATACCCGTTTCCACGTCAGCTTTAATTCGTGCGTGTCAGCTGGCGTTCACAGCAGGCTTCGCGTTCTGGCTAGTGAAACAGAAATTCACAGCCTATTCAATAAACTCCGTCGTTTTGGTTACCGCCGGAGGGGCAATTTTGGCATTACATACAAGTGGAGATCGCCGTGCCGGAGAATCCAATAGAGAATACATCGAAGGGTTCTTAACGACCGTAGCAGCGTCGGTTGTGTACGGATTCATTCTGCCCCTTGTGGAATTGACCTACAAAAAAGCTCGTCAGCAAATCACCTACACTTTAGTCCTTGAAGTTCAGTTGATTATGTCATTGTTCGCCACCCTCGTATGCTCCATTGCCATGTTAATCAACAACGATTTCCAGGTAATAATAATTAAAACATTAATCAAATACTATTTGTTACGTTATAATAACAATCATAACGATCATAAATCACTTATGGGAAGACAAATTCAGTATTATATCACTTTATGGATGAAGAAAATCCCAATAAGCTAGTAGCTTAAACACAAGACTTGTCTTACTTTAATACCAATTTAAATCATCCAATTGATTGATGGAAAGATAAATTTAATATTATATCACTTAGTTTGTTCATTTAACATATTGATTTTTTTTCTTTTTTTTTTGCATGTATGTAGATGATTGCAATGGAAGCTGAAGCATTTGGATTAGGAAAGGCCAAATACTATGTGATATTAGTATTGAGTGCCATAATTTGGCAAGGTTTCTTTTTAGGAGTGATAGGGGTTATTTTCAGCTCCTCATCTTTTTTTTCCGGGATAATAATCGCCGTCCTATTGCCGGTCACTGAGATTCTTGCCGTTGTCACTTTCAATGAGAAGTTTCAAGCGGAAAAAACTATTTCTTTGATACTCAATCTTTGGGGTTTTGTATCTTATTTTTATGGTGAAATTAAACACAACAAAAAAAAGATGAAGAACTTAGAGCTCCAAAGACGTGCAGAAACAACGACTACTCAAATTACAAACATCTAA

mRNA sequence

ATGGACCAACAACAACAACAAACCACCCCCGCCATGAGAAGGACTCTTCTTGTTTTCAATTGCTTTTTGTTAGCCGTCGGTACCTGCGGTGGTCCTTTGCTTATGCGTTTGTATTTCCTTCACGGCGGAAACCGCGTCTGGCTCTCCACCTTTCTAGAAACCGCCGGATGTCCAATTATTTTCATCCCCTTGCTAATTAGTTACATTCATCGCCGCCGGCGTCGTGCCCACTCCCTTAACCCATCGGAATCTACCAACTCCACCGAGATGATCTTCATGAAGTCACGCCTATTTTTAGCCTCCGGTGTCATCGGCATCATCACCGGCTTCGTGGATTTCTTATACGCTTACGGCGTCGCTCGAATACCCGTTTCCACGTCAGCTTTAATTCGTGCGTGTCAGCTGGCGTTCACAGCAGGCTTCGCGTTCTGGCTAGTGAAACAGAAATTCACAGCCTATTCAATAAACTCCGTCGTTTTGGTTACCGCCGGAGGGGCAATTTTGGCATTACATACAAGTGGAGATCGCCGTGCCGGAGAATCCAATAGAGAATACATCGAAGGGTTCTTAACGACCGTAGCAGCGTCGGTTGTGTACGGATTCATTCTGCCCCTTGTGGAATTGACCTACAAAAAAGCTCGTCAGCAAATCACCTACACTTTAGTCCTTGAAGTTCAGTTGATTATGTCATTGTTCGCCACCCTCGTATGCTCCATTGCCATGTTAATCAACAACGATTTCCAGATGATTGCAATGGAAGCTGAAGCATTTGGATTAGGAAAGGCCAAATACTATGTGATATTAGTATTGAGTGCCATAATTTGGCAAGGTTTCTTTTTAGGAGTGATAGGGGTTATTTTCAGCTCCTCATCTTTTTTTTCCGGGATAATAATCGCCGTCCTATTGCCGGTCACTGAGATTCTTGCCGTTGTCACTTTCAATGAGAAGTTTCAAGCGGAAAAAACTATTTCTTTGATACTCAATCTTTGGGGTTTTGTATCTTATTTTTATGGTGAAATTAAACACAACAAAAAAAAGATGAAGAACTTAGAGCTCCAAAGACGTGCAGAAACAACGACTACTCAAATTACAAACATCTAA

Coding sequence (CDS)

ATGGACCAACAACAACAACAAACCACCCCCGCCATGAGAAGGACTCTTCTTGTTTTCAATTGCTTTTTGTTAGCCGTCGGTACCTGCGGTGGTCCTTTGCTTATGCGTTTGTATTTCCTTCACGGCGGAAACCGCGTCTGGCTCTCCACCTTTCTAGAAACCGCCGGATGTCCAATTATTTTCATCCCCTTGCTAATTAGTTACATTCATCGCCGCCGGCGTCGTGCCCACTCCCTTAACCCATCGGAATCTACCAACTCCACCGAGATGATCTTCATGAAGTCACGCCTATTTTTAGCCTCCGGTGTCATCGGCATCATCACCGGCTTCGTGGATTTCTTATACGCTTACGGCGTCGCTCGAATACCCGTTTCCACGTCAGCTTTAATTCGTGCGTGTCAGCTGGCGTTCACAGCAGGCTTCGCGTTCTGGCTAGTGAAACAGAAATTCACAGCCTATTCAATAAACTCCGTCGTTTTGGTTACCGCCGGAGGGGCAATTTTGGCATTACATACAAGTGGAGATCGCCGTGCCGGAGAATCCAATAGAGAATACATCGAAGGGTTCTTAACGACCGTAGCAGCGTCGGTTGTGTACGGATTCATTCTGCCCCTTGTGGAATTGACCTACAAAAAAGCTCGTCAGCAAATCACCTACACTTTAGTCCTTGAAGTTCAGTTGATTATGTCATTGTTCGCCACCCTCGTATGCTCCATTGCCATGTTAATCAACAACGATTTCCAGATGATTGCAATGGAAGCTGAAGCATTTGGATTAGGAAAGGCCAAATACTATGTGATATTAGTATTGAGTGCCATAATTTGGCAAGGTTTCTTTTTAGGAGTGATAGGGGTTATTTTCAGCTCCTCATCTTTTTTTTCCGGGATAATAATCGCCGTCCTATTGCCGGTCACTGAGATTCTTGCCGTTGTCACTTTCAATGAGAAGTTTCAAGCGGAAAAAACTATTTCTTTGATACTCAATCTTTGGGGTTTTGTATCTTATTTTTATGGTGAAATTAAACACAACAAAAAAAAGATGAAGAACTTAGAGCTCCAAAGACGTGCAGAAACAACGACTACTCAAATTACAAACATCTAA

Protein sequence

MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQITNI*
Homology
BLAST of CSPI01G11520 vs. ExPASy Swiss-Prot
Match: Q9FZ96 (Purine permease 1 OS=Arabidopsis thaliana OX=3702 GN=PUP1 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 7.1e-105
Identity = 197/335 (58.81%), Postives = 256/335 (76.42%), Query Frame = 0

Query: 12  MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHR 71
           M+  L++ NC +L +GTCGGPLL RLYF +GG R+W  +FL TAG PII IPLL+S++ R
Sbjct: 1   MKNGLIIINCIILTIGTCGGPLLTRLYFTNGGKRIWFMSFLSTAGFPIILIPLLVSFLSR 60

Query: 72  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 131
           RR   +  N +E+   T++  M++ LF+AS VIG++TG  ++LY+YG+A +PVSTS+LI 
Sbjct: 61  RRSNRNP-NNAENKRKTKLFLMETPLFIASIVIGLLTGLDNYLYSYGLAYLPVSTSSLII 120

Query: 132 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 191
             QLAF A FAF LVKQKFT +SIN+VVL+T G  ILALH+ GD+ A ES +EY+ GFL 
Sbjct: 121 GTQLAFNALFAFLLVKQKFTPFSINAVVLLTVGIGILALHSDGDKPAKESKKEYVVGFLM 180

Query: 192 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIA 251
           TV A+++Y FILPLVELTYKKARQ+IT+ LVLE+Q++M L AT  C I M I  DF++IA
Sbjct: 181 TVVAALLYAFILPLVELTYKKARQEITFPLVLEIQMVMCLAATFFCVIGMFIVGDFKVIA 240

Query: 252 MEAEAFGL-GKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAV 311
            EA  F + G   YY ++V++ IIWQGFFLG IG++F +SS  SG++I+VLLPVTE+ AV
Sbjct: 241 REAREFKIGGSVFYYALIVITGIIWQGFFLGAIGIVFCASSLASGVLISVLLPVTEVFAV 300

Query: 312 VTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKK 346
           V F EKFQAEK +SL+L+LWGFVSYFYGE K  KK
Sbjct: 301 VCFREKFQAEKGVSLLLSLWGFVSYFYGEFKSGKK 334

BLAST of CSPI01G11520 vs. ExPASy Swiss-Prot
Match: Q9FZ95 (Purine permease 3 OS=Arabidopsis thaliana OX=3702 GN=PUP3 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 6.0e-104
Identity = 197/353 (55.81%), Postives = 267/353 (75.64%), Query Frame = 0

Query: 12  MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHR 71
           M + L++ NC +LA+G CGGPL+MRLYF +GG R+W STFLETAG P+IFIPLL SYI R
Sbjct: 1   MVKALVIINCIILAIGNCGGPLIMRLYFNNGGKRIWFSTFLETAGFPVIFIPLLFSYITR 60

Query: 72  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 131
           RR        +   +ST    +K RL +A+ ++GI++GF ++LYAYG+A +PVST+ALI 
Sbjct: 61  RR-------SNNVGDSTSFFLIKPRLLIAAVIVGILSGFDNYLYAYGIAYLPVSTAALII 120

Query: 132 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 191
           A QLAF A F+F++VK KFT ++IN+VVL+T G A+L +HT  D+   E++++YI GFL 
Sbjct: 121 ASQLAFIAIFSFFMVKHKFTPFTINAVVLLTVGAAVLGMHTETDKPVHETHKQYITGFLI 180

Query: 192 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIA 251
           TVAA+V+Y FILPLVEL Y+KA+Q ++YTLVLE QLI+ L A++V  I M I  DF+ + 
Sbjct: 181 TVAAAVMYAFILPLVELAYQKAKQTMSYTLVLEFQLILCLLASIVSVIGMFIAGDFKALP 240

Query: 252 MEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVV 311
            EA  F LG+A +YV+ V SAIIWQGFFLG IG+IFS+SS  SGI+I+VLLP+TE+LAV+
Sbjct: 241 KEAREFKLGEALFYVVAVFSAIIWQGFFLGAIGLIFSTSSLVSGIMISVLLPITEVLAVI 300

Query: 312 TFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQIT 365
            ++EKFQAEK +SL L+LWGFVSYFYGEIK  + K + ++ +   ET  + ++
Sbjct: 301 FYHEKFQAEKGLSLALSLWGFVSYFYGEIKSGEDK-RRIQQEESQETEQSSLS 345

BLAST of CSPI01G11520 vs. ExPASy Swiss-Prot
Match: Q94GB1 (Purine permease 2 OS=Arabidopsis thaliana OX=3702 GN=PUP2 PE=1 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.3e-95
Identity = 178/345 (51.59%), Postives = 251/345 (72.75%), Query Frame = 0

Query: 12  MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHR 71
           M+  L++ NC  LA+G CGGPL+MRLYF +GG R+W  +FL+T GCP+IF PLL+S++ R
Sbjct: 3   MKTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRR 62

Query: 72  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 131
           RR         E   +T    MK  LF+A+ V+G++ GF ++LY+YG+A IPVST++LI 
Sbjct: 63  RR-------CLEEQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLII 122

Query: 132 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 191
           + QL FTA FAF++VKQKFT ++IN++VL+T G  +LAL++  D+ A E+++EY+ GF+ 
Sbjct: 123 SAQLGFTALFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIM 182

Query: 192 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDF---- 251
           T+ A+++YGFILPLVEL+YKK+ Q+ITYTL LE Q+++   AT VC + ML   DF    
Sbjct: 183 TLGAALLYGFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVKH 242

Query: 252 -------QMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAV 311
                  ++IA EA  F LG++ YYV++V +AIIWQ FF+G IG+IF +SS  SGI+++ 
Sbjct: 243 ALFIFKNRVIAGEARDFKLGESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSA 302

Query: 312 LLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKK 346
           LLPVT ILAV+ F EKFQA K ++L L+LWG VSYFYG++K  +K
Sbjct: 303 LLPVTVILAVICFQEKFQAGKGVALALSLWGSVSYFYGQVKSEEK 340

BLAST of CSPI01G11520 vs. ExPASy Swiss-Prot
Match: Q9LPF6 (Probable purine permease 11 OS=Arabidopsis thaliana OX=3702 GN=PUP11 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.1e-48
Identity = 116/322 (36.02%), Postives = 189/322 (58.70%), Query Frame = 0

Query: 16  LLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHRRRRR 75
           L+  N F L  G     LL R Y+  GGN  W++T ++TA  PI++IPLL+         
Sbjct: 45  LVSVNIFFLIGGQAASVLLGRFYYDEGGNSKWMATLVQTAAFPILYIPLLL--------- 104

Query: 76  AHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIRACQL 135
              L  S S  S+E       + L   ++G+I    + LY+ G+  +  ST +LI A QL
Sbjct: 105 ---LPSSASVESSESSCSLKYIVLIYVLLGVIIAGDNMLYSVGLLYLSASTYSLICATQL 164

Query: 136 AFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLTTVAA 195
           AF A F++++  QKFTA  +NSVVL++   A++AL+   D  +G S  +YI GF+ T+AA
Sbjct: 165 AFNAVFSYFINAQKFTALILNSVVLLSFSAALIALNDDADTPSGVSRSKYIVGFVCTLAA 224

Query: 196 SVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIAMEAE 255
           S +Y  +L L++ +++K  ++ T+++VLE+Q+  SL AT V  I +  + +++ +  E E
Sbjct: 225 SALYSLLLSLMQFSFEKILKRETFSVVLEMQIYTSLVATCVSVIGLFASGEWRTLHGEME 284

Query: 256 AFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVVTFNE 315
            +  G+A Y + LV +A+ WQ   +GV+G+IF  +S FS +I  + L VT + A+V F +
Sbjct: 285 GYHKGQASYVLTLVWTAVTWQVCSVGVVGLIFLVTSLFSNVISTLSLAVTPLAALVVFRD 344

Query: 316 KFQAEKTISLILNLWGFVSYFY 338
           K    K +++++ +WGF SY Y
Sbjct: 345 KMSGVKIMAMLIAIWGFASYVY 354

BLAST of CSPI01G11520 vs. ExPASy Swiss-Prot
Match: O49726 (Purine permease 21 OS=Arabidopsis thaliana OX=3702 GN=PUP21 PE=2 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 1.1e-44
Identity = 116/332 (34.94%), Postives = 187/332 (56.33%), Query Frame = 0

Query: 22  FLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISY-IHRRRRRAHSLN 81
           F +  G     +L RLY+ +GGN  WL+T ++  G PI+    L+S   H   +R   L 
Sbjct: 52  FFVISGQSVATILGRLYYENGGNSKWLATVVQLVGFPILLPYHLLSVKTHTTTQRDGKLT 111

Query: 82  PSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIRACQLAFTAG 141
              S  +  ++++         V+G++ G   +LY+ G+  +PVST +LI A QLAFTA 
Sbjct: 112 ---SLRNRALVYI---------VLGLLVGAACYLYSIGLLYLPVSTLSLICASQLAFTAF 171

Query: 142 FAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLTTVAASVVYG 201
           F++ L  QK T   +NS+ L+T    +LA +         +  EY++GF+ TV AS  +G
Sbjct: 172 FSYLLNSQKLTPIILNSLFLLTISSTLLAFNNEESDSKKVTKGEYVKGFVCTVGASAGFG 231

Query: 202 FILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIAMEAEAFGLG 261
            +L L +L ++K  ++ T++ V+ + + MSL A+ V  + +  +++++ ++ E E + LG
Sbjct: 232 LLLSLQQLAFRKVLKKQTFSEVINMIIYMSLVASCVSVVGLFASSEWKTLSSEMENYKLG 291

Query: 262 KAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVVTFNEKFQAE 321
           K  Y + LV +A+ WQ F +G  G+IF  SS FS  I A+ LPV  ILAV+ F++K    
Sbjct: 292 KVSYVMNLVWTAVTWQVFSIGCTGLIFELSSLFSNAISALGLPVVPILAVIIFHDKMNGL 351

Query: 322 KTISLILNLWGFVSYFYGEIKHNKKKMKNLEL 353
           K IS+IL +WGFVSY Y +        K+ E+
Sbjct: 352 KVISMILAIWGFVSYVYQQYLDETNLKKSNEI 371

BLAST of CSPI01G11520 vs. ExPASy TrEMBL
Match: A0A0A0LRS6 (Probable purine permease OS=Cucumis sativus OX=3659 GN=Csa_1G071290 PE=3 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 5.3e-196
Identity = 365/366 (99.73%), Postives = 365/366 (99.73%), Query Frame = 0

Query: 1   MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPII 60
           MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLET GCPII
Sbjct: 1   MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETGGCPII 60

Query: 61  FIPLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVA 120
           FIPLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVA
Sbjct: 61  FIPLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVA 120

Query: 121 RIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGE 180
           RIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGE
Sbjct: 121 RIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGE 180

Query: 181 SNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIA 240
           SNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIA
Sbjct: 181 SNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIA 240

Query: 241 MLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAV 300
           MLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAV
Sbjct: 241 MLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAV 300

Query: 301 LLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTT 360
           LLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTT
Sbjct: 301 LLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTT 360

Query: 361 TQITNI 367
           TQITNI
Sbjct: 361 TQITNI 366

BLAST of CSPI01G11520 vs. ExPASy TrEMBL
Match: A0A1S3BFE5 (Probable purine permease OS=Cucumis melo OX=3656 GN=LOC103489275 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 8.2e-173
Identity = 327/367 (89.10%), Postives = 345/367 (94.01%), Query Frame = 0

Query: 1   MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPII 60
           MDQQQQ TT AMRR LLVFNCFLLAVGTCGGPL+MRLYFLHGGNRVWLS+FL+T GCPII
Sbjct: 1   MDQQQQNTT-AMRRPLLVFNCFLLAVGTCGGPLIMRLYFLHGGNRVWLSSFLQTGGCPII 60

Query: 61  FIPLLISYIHRRR--RRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYG 120
           FIPLLISYIHRRR  R  +SLNPSE TNSTEMIFMK RLFLAS VIGIITGFVDFLYAYG
Sbjct: 61  FIPLLISYIHRRRRPRAQYSLNPSEPTNSTEMIFMKPRLFLASSVIGIITGFVDFLYAYG 120

Query: 121 VARIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRA 180
           VA++PVSTSALIRACQLAFTAGFAFWLVKQKFT YSIN+VVLVTAGGAILALHTSGDRRA
Sbjct: 121 VAQLPVSTSALIRACQLAFTAGFAFWLVKQKFTPYSINAVVLVTAGGAILALHTSGDRRA 180

Query: 181 GESNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCS 240
           GE+NR+YI GFLTTVAA+VVYGFILPLVEL+YK+ARQQITYTLVLEVQ  MSLFATLVC+
Sbjct: 181 GEANRDYIAGFLTTVAAAVVYGFILPLVELSYKRARQQITYTLVLEVQFFMSLFATLVCT 240

Query: 241 IAMLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIII 300
           IAM+INNDFQ+I  EAEAFGLGKAKYYVILVLSAIIWQ FFLGV+GVIF SSSFFS I+I
Sbjct: 241 IAMVINNDFQVIPTEAEAFGLGKAKYYVILVLSAIIWQSFFLGVVGVIFCSSSFFSEIVI 300

Query: 301 AVLLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAET 360
           AVLLPVTEILAV+ FNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMK LELQRRAET
Sbjct: 301 AVLLPVTEILAVIIFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMK-LELQRRAET 360

Query: 361 TTTQITN 366
           TTTQITN
Sbjct: 361 TTTQITN 365

BLAST of CSPI01G11520 vs. ExPASy TrEMBL
Match: A0A5A7V9X1 (Probable purine permease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004570 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.5e-153
Identity = 291/364 (79.95%), Postives = 325/364 (89.29%), Query Frame = 0

Query: 3   QQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFI 62
           QQ QQ T A++RTLL+FNCFLLAVG CGGPL+MRLYF+HGGNRVWLS+FLET G PIIFI
Sbjct: 6   QQHQQNTSAVKRTLLIFNCFLLAVGNCGGPLVMRLYFVHGGNRVWLSSFLETGGWPIIFI 65

Query: 63  PLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARI 122
           PLLISYI  RRRR  +L+PS S +S+++IFMK RLFLAS VIGI+TGF D+LYAYGVAR+
Sbjct: 66  PLLISYI--RRRRLAALDPSGSADSSQIIFMKPRLFLASAVIGILTGFDDYLYAYGVARL 125

Query: 123 PVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESN 182
           PVSTSALI ACQLAFTAGFAF LVKQKFT+YSIN+VVL+T GGA+LALHTSGDR AGESN
Sbjct: 126 PVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESN 185

Query: 183 REYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAML 242
           ++YI GFL TVAA+VVYGFILPLVELTYKKARQQITYTLVLE QLIMSLFAT+VC I ML
Sbjct: 186 KDYIAGFLMTVAAAVVYGFILPLVELTYKKARQQITYTLVLEYQLIMSLFATIVCVIGML 245

Query: 243 INNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLL 302
           INNDFQ+I  EAE FGLGK +YY++LVLSAI+WQGFFLG IGVIFSSSS FSGI+IAVLL
Sbjct: 246 INNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLL 305

Query: 303 PVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQ 362
           PVTEILAV+ FNEKFQAEK +SL LNLWGFVSYFYGEIK+NKK  K LELQR  ET +TQ
Sbjct: 306 PVTEILAVIIFNEKFQAEKAVSLALNLWGFVSYFYGEIKNNKK--KKLELQRYEETASTQ 365

Query: 363 ITNI 367
           ITN+
Sbjct: 366 ITNV 365

BLAST of CSPI01G11520 vs. ExPASy TrEMBL
Match: A0A1S3BC92 (Probable purine permease OS=Cucumis melo OX=3656 GN=LOC103488091 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.5e-153
Identity = 291/364 (79.95%), Postives = 325/364 (89.29%), Query Frame = 0

Query: 3   QQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFI 62
           QQ QQ T A++RTLL+FNCFLLAVG CGGPL+MRLYF+HGGNRVWLS+FLET G PIIFI
Sbjct: 6   QQHQQNTSAVKRTLLIFNCFLLAVGNCGGPLVMRLYFVHGGNRVWLSSFLETGGWPIIFI 65

Query: 63  PLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARI 122
           PLLISYI  RRRR  +L+PS S +S+++IFMK RLFLAS VIGI+TGF D+LYAYGVAR+
Sbjct: 66  PLLISYI--RRRRLAALDPSGSADSSQIIFMKPRLFLASAVIGILTGFDDYLYAYGVARL 125

Query: 123 PVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESN 182
           PVSTSALI ACQLAFTAGFAF LVKQKFT+YSIN+VVL+T GGA+LALHTSGDR AGESN
Sbjct: 126 PVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESN 185

Query: 183 REYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAML 242
           ++YI GFL TVAA+VVYGFILPLVELTYKKARQQITYTLVLE QLIMSLFAT+VC I ML
Sbjct: 186 KDYIAGFLMTVAAAVVYGFILPLVELTYKKARQQITYTLVLEYQLIMSLFATIVCVIGML 245

Query: 243 INNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLL 302
           INNDFQ+I  EAE FGLGK +YY++LVLSAI+WQGFFLG IGVIFSSSS FSGI+IAVLL
Sbjct: 246 INNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLL 305

Query: 303 PVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQ 362
           PVTEILAV+ FNEKFQAEK +SL LNLWGFVSYFYGEIK+NKK  K LELQR  ET +TQ
Sbjct: 306 PVTEILAVIIFNEKFQAEKAVSLALNLWGFVSYFYGEIKNNKK--KKLELQRYEETASTQ 365

Query: 363 ITNI 367
           ITN+
Sbjct: 366 ITNV 365

BLAST of CSPI01G11520 vs. ExPASy TrEMBL
Match: A0A0A0LPJ9 (Probable purine permease OS=Cucumis sativus OX=3659 GN=Csa_2G369730 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.7e-152
Identity = 286/364 (78.57%), Postives = 323/364 (88.74%), Query Frame = 0

Query: 3   QQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFI 62
           +QQQQ TP+++RTLL+FNC LLAVG CGGPL+MRLYF+HGG RVWLS+ LET G PIIFI
Sbjct: 4   EQQQQNTPSVKRTLLIFNCCLLAVGNCGGPLIMRLYFVHGGKRVWLSSCLETGGWPIIFI 63

Query: 63  PLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARI 122
           PLLISYIHRRR  A  L+PS S NS + IFMK RLFLAS  IGI+TGF D+LYAYGVAR+
Sbjct: 64  PLLISYIHRRRLAA--LDPSGSPNSAQFIFMKPRLFLASAFIGILTGFDDYLYAYGVARL 123

Query: 123 PVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESN 182
           PVSTSALI ACQLAFTAGFAF LVKQKFT+YSIN+VVL+T GGA+LALHTSGDR AGESN
Sbjct: 124 PVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESN 183

Query: 183 REYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAML 242
           ++YI GFL TVAA+VVYGF+LPLVELTYKKARQQITYTLVLE QLIMSLFAT+VC+I ML
Sbjct: 184 KDYIAGFLMTVAAAVVYGFVLPLVELTYKKARQQITYTLVLEFQLIMSLFATIVCAIGML 243

Query: 243 INNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLL 302
           INNDFQ+I  EAEAFGLGK +YY++LVLSAI+WQGFFLG IGVIFSSSS FSGI+IAVLL
Sbjct: 244 INNDFQVIPREAEAFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLL 303

Query: 303 PVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQ 362
           PVTEILAV+ FNE+FQAEK +SL LNLWGF+SYFYGEIKHNK+  K LELQR  ETT+TQ
Sbjct: 304 PVTEILAVIIFNERFQAEKGVSLALNLWGFLSYFYGEIKHNKR--KKLELQRYEETTSTQ 363

Query: 363 ITNI 367
           + N+
Sbjct: 364 VANV 363

BLAST of CSPI01G11520 vs. NCBI nr
Match: XP_004146629.2 (purine permease 3 [Cucumis sativus])

HSP 1 Score: 693.3 bits (1788), Expect = 1.1e-195
Identity = 365/366 (99.73%), Postives = 365/366 (99.73%), Query Frame = 0

Query: 1   MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPII 60
           MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLET GCPII
Sbjct: 1   MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETGGCPII 60

Query: 61  FIPLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVA 120
           FIPLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVA
Sbjct: 61  FIPLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVA 120

Query: 121 RIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGE 180
           RIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGE
Sbjct: 121 RIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGE 180

Query: 181 SNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIA 240
           SNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIA
Sbjct: 181 SNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIA 240

Query: 241 MLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAV 300
           MLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAV
Sbjct: 241 MLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAV 300

Query: 301 LLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTT 360
           LLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTT
Sbjct: 301 LLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTT 360

Query: 361 TQITNI 367
           TQITNI
Sbjct: 361 TQITNI 366

BLAST of CSPI01G11520 vs. NCBI nr
Match: KAE8652695.1 (hypothetical protein Csa_014148 [Cucumis sativus])

HSP 1 Score: 638.3 bits (1645), Expect = 4.2e-179
Identity = 341/355 (96.06%), Postives = 341/355 (96.06%), Query Frame = 0

Query: 12  MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHR 71
           MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLET GCPIIFIPLLISYIHR
Sbjct: 1   MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETGGCPIIFIPLLISYIHR 60

Query: 72  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 131
           RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR
Sbjct: 61  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 120

Query: 132 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 191
           ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT
Sbjct: 121 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 180

Query: 192 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIA 251
           TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATL             MIA
Sbjct: 181 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATL-------------MIA 240

Query: 252 MEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVV 311
           MEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVV
Sbjct: 241 MEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVV 300

Query: 312 TFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQITNI 367
           TFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQITNI
Sbjct: 301 TFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQITNI 342

BLAST of CSPI01G11520 vs. NCBI nr
Match: XP_008446590.1 (PREDICTED: purine permease 3-like [Cucumis melo])

HSP 1 Score: 616.3 bits (1588), Expect = 1.7e-172
Identity = 327/367 (89.10%), Postives = 345/367 (94.01%), Query Frame = 0

Query: 1   MDQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPII 60
           MDQQQQ TT AMRR LLVFNCFLLAVGTCGGPL+MRLYFLHGGNRVWLS+FL+T GCPII
Sbjct: 1   MDQQQQNTT-AMRRPLLVFNCFLLAVGTCGGPLIMRLYFLHGGNRVWLSSFLQTGGCPII 60

Query: 61  FIPLLISYIHRRR--RRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYG 120
           FIPLLISYIHRRR  R  +SLNPSE TNSTEMIFMK RLFLAS VIGIITGFVDFLYAYG
Sbjct: 61  FIPLLISYIHRRRRPRAQYSLNPSEPTNSTEMIFMKPRLFLASSVIGIITGFVDFLYAYG 120

Query: 121 VARIPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRA 180
           VA++PVSTSALIRACQLAFTAGFAFWLVKQKFT YSIN+VVLVTAGGAILALHTSGDRRA
Sbjct: 121 VAQLPVSTSALIRACQLAFTAGFAFWLVKQKFTPYSINAVVLVTAGGAILALHTSGDRRA 180

Query: 181 GESNREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCS 240
           GE+NR+YI GFLTTVAA+VVYGFILPLVEL+YK+ARQQITYTLVLEVQ  MSLFATLVC+
Sbjct: 181 GEANRDYIAGFLTTVAAAVVYGFILPLVELSYKRARQQITYTLVLEVQFFMSLFATLVCT 240

Query: 241 IAMLINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIII 300
           IAM+INNDFQ+I  EAEAFGLGKAKYYVILVLSAIIWQ FFLGV+GVIF SSSFFS I+I
Sbjct: 241 IAMVINNDFQVIPTEAEAFGLGKAKYYVILVLSAIIWQSFFLGVVGVIFCSSSFFSEIVI 300

Query: 301 AVLLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAET 360
           AVLLPVTEILAV+ FNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMK LELQRRAET
Sbjct: 301 AVLLPVTEILAVIIFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMK-LELQRRAET 360

Query: 361 TTTQITN 366
           TTTQITN
Sbjct: 361 TTTQITN 365

BLAST of CSPI01G11520 vs. NCBI nr
Match: XP_008444875.1 (PREDICTED: purine permease 3-like [Cucumis melo] >KAA0065122.1 purine permease 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 552.4 bits (1422), Expect = 3.0e-153
Identity = 291/364 (79.95%), Postives = 325/364 (89.29%), Query Frame = 0

Query: 3   QQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFI 62
           QQ QQ T A++RTLL+FNCFLLAVG CGGPL+MRLYF+HGGNRVWLS+FLET G PIIFI
Sbjct: 6   QQHQQNTSAVKRTLLIFNCFLLAVGNCGGPLVMRLYFVHGGNRVWLSSFLETGGWPIIFI 65

Query: 63  PLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARI 122
           PLLISYI  RRRR  +L+PS S +S+++IFMK RLFLAS VIGI+TGF D+LYAYGVAR+
Sbjct: 66  PLLISYI--RRRRLAALDPSGSADSSQIIFMKPRLFLASAVIGILTGFDDYLYAYGVARL 125

Query: 123 PVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESN 182
           PVSTSALI ACQLAFTAGFAF LVKQKFT+YSIN+VVL+T GGA+LALHTSGDR AGESN
Sbjct: 126 PVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTIGGAVLALHTSGDRPAGESN 185

Query: 183 REYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAML 242
           ++YI GFL TVAA+VVYGFILPLVELTYKKARQQITYTLVLE QLIMSLFAT+VC I ML
Sbjct: 186 KDYIAGFLMTVAAAVVYGFILPLVELTYKKARQQITYTLVLEYQLIMSLFATIVCVIGML 245

Query: 243 INNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLL 302
           INNDFQ+I  EAE FGLGK +YY++LVLSAI+WQGFFLG IGVIFSSSS FSGI+IAVLL
Sbjct: 246 INNDFQVIPREAETFGLGKFRYYLVLVLSAILWQGFFLGAIGVIFSSSSLFSGIVIAVLL 305

Query: 303 PVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQ 362
           PVTEILAV+ FNEKFQAEK +SL LNLWGFVSYFYGEIK+NKK  K LELQR  ET +TQ
Sbjct: 306 PVTEILAVIIFNEKFQAEKAVSLALNLWGFVSYFYGEIKNNKK--KKLELQRYEETASTQ 365

Query: 363 ITNI 367
           ITN+
Sbjct: 366 ITNV 365

BLAST of CSPI01G11520 vs. NCBI nr
Match: XP_038885544.1 (purine permease 3-like [Benincasa hispida])

HSP 1 Score: 550.4 bits (1417), Expect = 1.1e-152
Identity = 289/365 (79.18%), Postives = 324/365 (88.77%), Query Frame = 0

Query: 2   DQQQQQTTPAMRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIF 61
           +QQ QQ T AM+RTLLVFNCFLLAVG CGGPL+MRLYF+HGG RVWLS+FLET G PIIF
Sbjct: 4   EQQPQQNTTAMKRTLLVFNCFLLAVGNCGGPLVMRLYFVHGGKRVWLSSFLETGGWPIIF 63

Query: 62  IPLLISYIHRRRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVAR 121
           IPL+ISYI+RRR  A  LNPS ST+S +MIFMK RLFLA+ VIGI+TGF D+LYAYGVAR
Sbjct: 64  IPLIISYINRRRVAA--LNPSVSTDSAKMIFMKPRLFLATAVIGILTGFDDYLYAYGVAR 123

Query: 122 IPVSTSALIRACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGES 181
           +PVSTSALI ACQLAFTAGFAF LVKQKFT+YSIN+VVL+T GGA+LALHTSGDR AGES
Sbjct: 124 LPVSTSALIIACQLAFTAGFAFLLVKQKFTSYSINAVVLLTMGGAVLALHTSGDRPAGES 183

Query: 182 NREYIEGFLTTVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAM 241
           NR+YI GFL TVAA+++YGF+LPLVELTY+KARQQITYTLVLE QLIMSLFAT+VC+I M
Sbjct: 184 NRDYIAGFLMTVAAAIMYGFVLPLVELTYRKARQQITYTLVLEFQLIMSLFATIVCTIGM 243

Query: 242 LINNDFQMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVL 301
           LINNDFQ+I  EAE FGLGK +YY++LVLSAIIWQ FFLG IGVIFSSSS FSGI+IAVL
Sbjct: 244 LINNDFQVIPREAEEFGLGKFRYYLVLVLSAIIWQAFFLGAIGVIFSSSSLFSGIVIAVL 303

Query: 302 LPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTT 361
           LPVTEILAV+ FNEKFQAEK +SL LNLWGFVSYFYGEIKHNKK  K LELQ+  ET  T
Sbjct: 304 LPVTEILAVIIFNEKFQAEKAVSLALNLWGFVSYFYGEIKHNKK--KKLELQKYEETRPT 363

Query: 362 QITNI 367
           QI+N+
Sbjct: 364 QISNV 364

BLAST of CSPI01G11520 vs. TAIR 10
Match: AT1G28230.1 (purine permease 1 )

HSP 1 Score: 382.1 bits (980), Expect = 5.0e-106
Identity = 197/335 (58.81%), Postives = 256/335 (76.42%), Query Frame = 0

Query: 12  MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHR 71
           M+  L++ NC +L +GTCGGPLL RLYF +GG R+W  +FL TAG PII IPLL+S++ R
Sbjct: 1   MKNGLIIINCIILTIGTCGGPLLTRLYFTNGGKRIWFMSFLSTAGFPIILIPLLVSFLSR 60

Query: 72  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 131
           RR   +  N +E+   T++  M++ LF+AS VIG++TG  ++LY+YG+A +PVSTS+LI 
Sbjct: 61  RRSNRNP-NNAENKRKTKLFLMETPLFIASIVIGLLTGLDNYLYSYGLAYLPVSTSSLII 120

Query: 132 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 191
             QLAF A FAF LVKQKFT +SIN+VVL+T G  ILALH+ GD+ A ES +EY+ GFL 
Sbjct: 121 GTQLAFNALFAFLLVKQKFTPFSINAVVLLTVGIGILALHSDGDKPAKESKKEYVVGFLM 180

Query: 192 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIA 251
           TV A+++Y FILPLVELTYKKARQ+IT+ LVLE+Q++M L AT  C I M I  DF++IA
Sbjct: 181 TVVAALLYAFILPLVELTYKKARQEITFPLVLEIQMVMCLAATFFCVIGMFIVGDFKVIA 240

Query: 252 MEAEAFGL-GKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAV 311
            EA  F + G   YY ++V++ IIWQGFFLG IG++F +SS  SG++I+VLLPVTE+ AV
Sbjct: 241 REAREFKIGGSVFYYALIVITGIIWQGFFLGAIGIVFCASSLASGVLISVLLPVTEVFAV 300

Query: 312 VTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKK 346
           V F EKFQAEK +SL+L+LWGFVSYFYGE K  KK
Sbjct: 301 VCFREKFQAEKGVSLLLSLWGFVSYFYGEFKSGKK 334

BLAST of CSPI01G11520 vs. TAIR 10
Match: AT1G28220.1 (purine permease 3 )

HSP 1 Score: 379.0 bits (972), Expect = 4.3e-105
Identity = 197/353 (55.81%), Postives = 267/353 (75.64%), Query Frame = 0

Query: 12  MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHR 71
           M + L++ NC +LA+G CGGPL+MRLYF +GG R+W STFLETAG P+IFIPLL SYI R
Sbjct: 1   MVKALVIINCIILAIGNCGGPLIMRLYFNNGGKRIWFSTFLETAGFPVIFIPLLFSYITR 60

Query: 72  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 131
           RR        +   +ST    +K RL +A+ ++GI++GF ++LYAYG+A +PVST+ALI 
Sbjct: 61  RR-------SNNVGDSTSFFLIKPRLLIAAVIVGILSGFDNYLYAYGIAYLPVSTAALII 120

Query: 132 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 191
           A QLAF A F+F++VK KFT ++IN+VVL+T G A+L +HT  D+   E++++YI GFL 
Sbjct: 121 ASQLAFIAIFSFFMVKHKFTPFTINAVVLLTVGAAVLGMHTETDKPVHETHKQYITGFLI 180

Query: 192 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIA 251
           TVAA+V+Y FILPLVEL Y+KA+Q ++YTLVLE QLI+ L A++V  I M I  DF+ + 
Sbjct: 181 TVAAAVMYAFILPLVELAYQKAKQTMSYTLVLEFQLILCLLASIVSVIGMFIAGDFKALP 240

Query: 252 MEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVV 311
            EA  F LG+A +YV+ V SAIIWQGFFLG IG+IFS+SS  SGI+I+VLLP+TE+LAV+
Sbjct: 241 KEAREFKLGEALFYVVAVFSAIIWQGFFLGAIGLIFSTSSLVSGIMISVLLPITEVLAVI 300

Query: 312 TFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKKKMKNLELQRRAETTTTQIT 365
            ++EKFQAEK +SL L+LWGFVSYFYGEIK  + K + ++ +   ET  + ++
Sbjct: 301 FYHEKFQAEKGLSLALSLWGFVSYFYGEIKSGEDK-RRIQQEESQETEQSSLS 345

BLAST of CSPI01G11520 vs. TAIR 10
Match: AT2G33750.2 (purine permease 2 )

HSP 1 Score: 359.8 bits (922), Expect = 2.7e-99
Identity = 178/334 (53.29%), Postives = 251/334 (75.15%), Query Frame = 0

Query: 12  MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHR 71
           M+  L++ NC  LA+G CGGPL+MRLYF +GG R+W  +FL+T GCP+IF PLL+S++ R
Sbjct: 3   MKTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRR 62

Query: 72  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 131
           RR         E   +T    MK  LF+A+ V+G++ GF ++LY+YG+A IPVST++LI 
Sbjct: 63  RR-------CLEEQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLII 122

Query: 132 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 191
           + QL FTA FAF++VKQKFT ++IN++VL+T G  +LAL++  D+ A E+++EY+ GF+ 
Sbjct: 123 SAQLGFTALFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIM 182

Query: 192 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIA 251
           T+ A+++YGFILPLVEL+YKK+ Q+ITYTL LE Q+++   AT VC + ML   DF++IA
Sbjct: 183 TLGAALLYGFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVIA 242

Query: 252 MEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVV 311
            EA  F LG++ YYV++V +AIIWQ FF+G IG+IF +SS  SGI+++ LLPVT ILAV+
Sbjct: 243 GEARDFKLGESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSALLPVTVILAVI 302

Query: 312 TFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKK 346
            F EKFQA K ++L L+LWG VSYFYG++K  +K
Sbjct: 303 CFQEKFQAGKGVALALSLWGSVSYFYGQVKSEEK 329

BLAST of CSPI01G11520 vs. TAIR 10
Match: AT2G33750.1 (purine permease 2 )

HSP 1 Score: 351.3 bits (900), Expect = 9.5e-97
Identity = 178/345 (51.59%), Postives = 251/345 (72.75%), Query Frame = 0

Query: 12  MRRTLLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHR 71
           M+  L++ NC  LA+G CGGPL+MRLYF +GG R+W  +FL+T GCP+IF PLL+S++ R
Sbjct: 3   MKTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRR 62

Query: 72  RRRRAHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIR 131
           RR         E   +T    MK  LF+A+ V+G++ GF ++LY+YG+A IPVST++LI 
Sbjct: 63  RR-------CLEEQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLII 122

Query: 132 ACQLAFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLT 191
           + QL FTA FAF++VKQKFT ++IN++VL+T G  +LAL++  D+ A E+++EY+ GF+ 
Sbjct: 123 SAQLGFTALFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIM 182

Query: 192 TVAASVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDF---- 251
           T+ A+++YGFILPLVEL+YKK+ Q+ITYTL LE Q+++   AT VC + ML   DF    
Sbjct: 183 TLGAALLYGFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVKH 242

Query: 252 -------QMIAMEAEAFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAV 311
                  ++IA EA  F LG++ YYV++V +AIIWQ FF+G IG+IF +SS  SGI+++ 
Sbjct: 243 ALFIFKNRVIAGEARDFKLGESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSA 302

Query: 312 LLPVTEILAVVTFNEKFQAEKTISLILNLWGFVSYFYGEIKHNKK 346
           LLPVT ILAV+ F EKFQA K ++L L+LWG VSYFYG++K  +K
Sbjct: 303 LLPVTVILAVICFQEKFQAGKGVALALSLWGSVSYFYGQVKSEEK 340

BLAST of CSPI01G11520 vs. TAIR 10
Match: AT1G44750.2 (purine permease 11 )

HSP 1 Score: 194.5 bits (493), Expect = 1.5e-49
Identity = 116/322 (36.02%), Postives = 189/322 (58.70%), Query Frame = 0

Query: 16  LLVFNCFLLAVGTCGGPLLMRLYFLHGGNRVWLSTFLETAGCPIIFIPLLISYIHRRRRR 75
           L+  N F L  G     LL R Y+  GGN  W++T ++TA  PI++IPLL+         
Sbjct: 33  LVSVNIFFLIGGQAASVLLGRFYYDEGGNSKWMATLVQTAAFPILYIPLLL--------- 92

Query: 76  AHSLNPSESTNSTEMIFMKSRLFLASGVIGIITGFVDFLYAYGVARIPVSTSALIRACQL 135
              L  S S  S+E       + L   ++G+I    + LY+ G+  +  ST +LI A QL
Sbjct: 93  ---LPSSASVESSESSCSLKYIVLIYVLLGVIIAGDNMLYSVGLLYLSASTYSLICATQL 152

Query: 136 AFTAGFAFWLVKQKFTAYSINSVVLVTAGGAILALHTSGDRRAGESNREYIEGFLTTVAA 195
           AF A F++++  QKFTA  +NSVVL++   A++AL+   D  +G S  +YI GF+ T+AA
Sbjct: 153 AFNAVFSYFINAQKFTALILNSVVLLSFSAALIALNDDADTPSGVSRSKYIVGFVCTLAA 212

Query: 196 SVVYGFILPLVELTYKKARQQITYTLVLEVQLIMSLFATLVCSIAMLINNDFQMIAMEAE 255
           S +Y  +L L++ +++K  ++ T+++VLE+Q+  SL AT V  I +  + +++ +  E E
Sbjct: 213 SALYSLLLSLMQFSFEKILKRETFSVVLEMQIYTSLVATCVSVIGLFASGEWRTLHGEME 272

Query: 256 AFGLGKAKYYVILVLSAIIWQGFFLGVIGVIFSSSSFFSGIIIAVLLPVTEILAVVTFNE 315
            +  G+A Y + LV +A+ WQ   +GV+G+IF  +S FS +I  + L VT + A+V F +
Sbjct: 273 GYHKGQASYVLTLVWTAVTWQVCSVGVVGLIFLVTSLFSNVISTLSLAVTPLAALVVFRD 332

Query: 316 KFQAEKTISLILNLWGFVSYFY 338
           K    K +++++ +WGF SY Y
Sbjct: 333 KMSGVKIMAMLIAIWGFASYVY 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZ967.1e-10558.81Purine permease 1 OS=Arabidopsis thaliana OX=3702 GN=PUP1 PE=1 SV=1[more]
Q9FZ956.0e-10455.81Purine permease 3 OS=Arabidopsis thaliana OX=3702 GN=PUP3 PE=2 SV=1[more]
Q94GB11.3e-9551.59Purine permease 2 OS=Arabidopsis thaliana OX=3702 GN=PUP2 PE=1 SV=1[more]
Q9LPF62.1e-4836.02Probable purine permease 11 OS=Arabidopsis thaliana OX=3702 GN=PUP11 PE=1 SV=1[more]
O497261.1e-4434.94Purine permease 21 OS=Arabidopsis thaliana OX=3702 GN=PUP21 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LRS65.3e-19699.73Probable purine permease OS=Cucumis sativus OX=3659 GN=Csa_1G071290 PE=3 SV=1[more]
A0A1S3BFE58.2e-17389.10Probable purine permease OS=Cucumis melo OX=3656 GN=LOC103489275 PE=3 SV=1[more]
A0A5A7V9X11.5e-15379.95Probable purine permease OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3BC921.5e-15379.95Probable purine permease OS=Cucumis melo OX=3656 GN=LOC103488091 PE=3 SV=1[more]
A0A0A0LPJ92.7e-15278.57Probable purine permease OS=Cucumis sativus OX=3659 GN=Csa_2G369730 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004146629.21.1e-19599.73purine permease 3 [Cucumis sativus][more]
KAE8652695.14.2e-17996.06hypothetical protein Csa_014148 [Cucumis sativus][more]
XP_008446590.11.7e-17289.10PREDICTED: purine permease 3-like [Cucumis melo][more]
XP_008444875.13.0e-15379.95PREDICTED: purine permease 3-like [Cucumis melo] >KAA0065122.1 purine permease 3... [more]
XP_038885544.11.1e-15279.18purine permease 3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G28230.15.0e-10658.81purine permease 1 [more]
AT1G28220.14.3e-10555.81purine permease 3 [more]
AT2G33750.22.7e-9953.29purine permease 2 [more]
AT2G33750.19.5e-9751.59purine permease 2 [more]
AT1G44750.21.5e-4936.02purine permease 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF16913PUNUTcoord: 16..345
e-value: 1.5E-113
score: 379.3
NoneNo IPR availablePANTHERPTHR31376:SF1PURINE PERMEASE 2coord: 3..352
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 95..172
IPR030182Purine permease, plantPANTHERPTHR31376OS09G0467300 PROTEIN-RELATEDcoord: 3..352

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G11520.1CSPI01G11520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:1904823 purine nucleobase transmembrane transport
biological_process GO:0015860 purine nucleoside transmembrane transport
biological_process GO:0048544 recognition of pollen
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0005345 purine nucleobase transmembrane transporter activity
molecular_function GO:0015211 purine nucleoside transmembrane transporter activity